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Papers by Ferdousy Begum

Scholars Journal of Applied Medical Sciences

Introduction: Lymphoma, a type of cancer affecting the lymphatic system, requires accurate diagno... more Introduction: Lymphoma, a type of cancer affecting the lymphatic system, requires accurate diagnostic methods for effective treatment. This study aimed to evaluate the validity of Cell Block Histopathology and Fine Needle Aspiration Cytology (FNAC) in the diagnosis of lymphoma. Methods: A total of 30 patients with clinically suspected lymphoproliferative disorder were included in the study. Both Cell Block Histopathology and FNAC were used to diagnose lymphoma in these patients. The results were then compared with the final diagnosis based on histopathology, immunochemistry, and clinical follow-up, which was considered the gold standard. Result: The study included 30 patients with clinically suspected lymphoproliferative disorder. The Cell Block Histopathology method diagnosed 12 patients (40%) as lymphoma positive, 11 patients (36.67%) as lymphoma negative, and 7 cases (23.33%) were inconclusive. The Fine Needle Aspiration Cytology (FNAC) method diagnosed 13 patients (43.33%) as ly...

Bangladesh Journal of Medical Science

Sclerosing stromal tumour (SST) is a rare benign sex cord stromal tumour occurring in women in th... more Sclerosing stromal tumour (SST) is a rare benign sex cord stromal tumour occurring in women in their second and third decades. Patients usually present with menstrual irregularity and pelvic pain. Microscopically, this tumour is characterized by epithelioid and spindle cells arranged in pseudolobules separated by areas with fibrous deposition of various amount. Presence of ‘staghorn like’ proliferating vasculature is the hallmark feature of this tumour. The main differential diagnoses are thecoma and fibroma. Immunohistochemistry can be used to differentiate these tumours. This relatively new entity should be kept in mind while reporting ovarian tumour in a young female. We have described SST in an 18 year old female in this case report. Bangladesh Journal of Medical Science Vol. 22 No. 03 July’23 Page : 712-717

Bangabandhu Sheikh Mujib Medical University Journal

Epstein Barr virus plays an important role in the pathogenesis of Hodgkin lymphoma. However, the ... more Epstein Barr virus plays an important role in the pathogenesis of Hodgkin lymphoma. However, the frequency of its association in Bangladeshi people has not been widely studied. The aim of this study was to determine the association of Epstein Barr virus association in Hodgkin Lymphoma through detection of Latent Membrane Protein 1 by immunohistochemistry and serum Epstein Barr virus viral capsid antigen IgG antibody titer by serology. This was a cross-sectional study in purposively selected 45 histologically diagnosed cases of Hodgkin lymphoma at Bangabandhu Sheikh Mujib Medical University, Dhaka, during the period of March 2017 to December 2018. Latent membrane protein 1 was positive in 71.1% of cases of Hodgkin lymphoma. Among these positive cases, 96% of cases had significantly raised titer of serum Epstein Barr virus viral capsid antigen IgG antibody (P<0.0001 obtained by Fisher’s Exact test), which had a male predominance. Mixed cellularity classical Hodgkin lymphoma showed ...

Neurology India, 2011

Rosai Dorfman disease (RDD), also known as sinus histiocytosis with massive lymphadenopathy, is a... more Rosai Dorfman disease (RDD), also known as sinus histiocytosis with massive lymphadenopathy, is a benign histiocytic proliferative disorder mainly affecting the lymph nodes. Although several cases of extra-nodal involvement have been reported previously, central nervous system involvement, particularly in the absence of nodal disease is extremely rare. We report a case of isolated intracranial RDD occurring in a relatively elder patient, which was shown by histological examination to have a dura-based involvement.

SAGE Open Medicine, 2021

Objectives: Epstein–Barr virus is a tumorigenic virus and has been extensively studied as a causa... more Objectives: Epstein–Barr virus is a tumorigenic virus and has been extensively studied as a causative agent for Hodgkin lymphoma. Although immunostaining of the tumor biopsy is the standard method for diagnosis of Epstein–Barr virus-driven Hodgkin lymphoma, the invasiveness of the procedure renders it difficult and less desirable for the patients. Therefore, we designed this study to evaluate the efficiency of plasma Epstein–Barr virus DNA detection as an alternative diagnostic and prognostic method for Epstein–Barr virus–associated Hodgkin lymphoma. Methods: This analytical cross-sectional study was conducted during March 2017 to December 2018 including 43 Hodgkin lymphoma patients diagnosed histopathologically followed by the latent membrane protein-1 immunohistochemistry to determine their Epstein–Barr virus association. Plasma Epstein–Barr virus DNA in these samples was measured using quantitative polymerase chain reaction (qPCR). Results: Of total, 29 (67.44%) patients tested p...

SAS Journal of Medicine, 2021

Introduction: Breast cancer is the most commonly happening female cancer and the leading cause of... more Introduction: Breast cancer is the most commonly happening female cancer and the leading cause of cancer deaths worldwide. Breast cancer is a heterogeneous disease and it encompasses a variety of entities with distinct morphological appearances and clinical behaviors. Triple-negative breast cancer (TNBC) represents 12.5– 15% of all breast cancers. Its molecular dissimilarity also brings a significantly different clinical course. It has been observed that basal-like breast cancers exhibit a gene expression profile very similar to that of myoepithelial cells. Aim of the study: To identify the basal like TNBC cases and correlate them with histomorphological grade. Material & Methods: This cross sectional study was conducted in the department of BSMMU from January 2016 to December 2017. A total of 41 cases previously diagnosed as TNBC by histological and immunohistochemical examinations were selected for the study. During collecting specimen, all relevant information were recorded syste...

Bangladesh Journal of Obstetrics & Gynaecology

Objective of the study: The increasing inter- and intra-observer variability in the diagnosis of ... more Objective of the study: The increasing inter- and intra-observer variability in the diagnosis of CIN by histopathology has led to the advent of different biomarkers. The aim of this study is to evaluate Ki-67 and p16 INK4a marker in differentiating CIN lesions and the clinical utility of it in the management of high grade squamous intraepithelial lesions (HSIL). Materials and methods: This cross-sectional study has been carried out among the biopsy proved cases of CIN2 and CIN3 in which P16 and Ki-67 immunostain was performed in the Colposcopy clinic, Bangabandhu Sheikh Mujib Medical University (BSMMU) and Department of Pathology, BSMMU respectively from 14-3-17 to 30-05-18. Thirty-two cases were incorporated in the study by purposive sampling. Data was analyzed using SPSS version 17. Results: Among 32 cases of HSIL, 25 were diagnosed as CIN 2 (78.13 %) and 7 cases as CIN 3 (21.87 %) by histopathology. All the cases were subjected to immunohistochemistry with P16 and Ki 67. Regardin...

Journal of Current and Advance Medical Research, 2016

Background: Colorectal carcinoma is one of the most common human malignancies worldwide. Objectiv... more Background: Colorectal carcinoma is one of the most common human malignancies worldwide. Objectives: The purpose of the present study was to see the clinico-demographic Characteristics of colorectal carcinoma. Methodology: This cross sectional study was carried out in the Department of Pathology at Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh during the period of November 2009 to July 2010. Patients with colorectal carcinoma from all ages and both sexes were included in the study. Detailed clinical information was obtained by taking history from patient's attendant's statement. Fresh unfixed specimens were obtained after surgical resection. Result: A total of 50 cases of colorectal adenocarcinoma from all ages and both sexes were included in the study. The mean age of the 50 cases was 47±14.8 years. Per rectal bleeding was the most common clinical presentation of colorectal carcinoma which was 20(40.0%) cases followed by abdominal pain, altered bowel habit, generalized weakness, anorexia and pallor and p/r bleeding and abdominal pain which were 8(16.0%) cases, 7(14.0%) cases, 6(12.0%) cases and 6(12.0%) cases respectively. Conclusion: In conclusion middle age male patients are the most vulnerable for colorectal carcinoma with common presenting features of per rectal bleeding, abdominal pain and altered bowel habit.

Introduction: Thyroglossal cyst is the most common congenital midline neck swelling. Most of the ... more Introduction: Thyroglossal cyst is the most common congenital midline neck swelling. Most of the cases are benign with rare malignant transformation is approximately in 1-2% cases. Only about 200 cases have been reported from different centers since 1911. Clinical presentation is similar to that of benign cyst and diagnosis is usually made incidentally during histopathological examination of excised cyst. Case report: We report a case of 46-year-old woman who underwent Sistrunk’s operation for thyroglossal cyst. Histopathological examination revealed papillary carcinoma. Thyroxine was added in suppressive dose and patient was on regular follow-up for 2 years. No thyroid abnormality was found preoperative cytology, scintigraphy as well as per-operative procedure and post-operative follow-up period. Conclusion: Sistrunk’s operation followed by suppressive dose of thyroxin and regular follow up is recommended for thyroglossal duct cyst carcinoma in asymptomatic incidental case without ...

Ovarian cancer is a devastating disease preoperative evaluation of the patients with an ovarian t... more Ovarian cancer is a devastating disease preoperative evaluation of the patients with an ovarian tumour is difficult. So frozen section biopsy of ovarian tumour is important to determine the extent of surgery. This retrospective cross-sectional study was carried out in the Department of Gynaecological Oncology, Bangabandhu Sheikh Mujib Medical University from August 2016 to July 2017 to determine the validity of frozen section biopsy in the diagnosis of ovarian tumour. Fifty cases of ovarian tumour underwent frozen section biopsy were included by purposive sampling. Histopathological finding was taken as the gold standard. Data was analyzed by SPSS version 16. The sensitivity of frozen section in the diagnosis of benign, borderline and malignant were 100%, 100% and 97.67% respectively as well as the specificities were 100%, 97.96% and 100% respectively. Similarly the accuracy, PPV, NPV for the benign, borderline and malignant ovarian tumour were also high except the borderline tumour...

Tuberculosis is a major public health problem in Bangladesh. Caseous necrosis is the key histomor... more Tuberculosis is a major public health problem in Bangladesh. Caseous necrosis is the key histomorphological feature for the diagnosis of tuberculosis. This cheesy material is described microscopically as amorphous granular debris. But it has a number of variations in color, appearance, inflammatory cellular content, cellular debris and apoptotic bodies. Does it contain Mycobacteria? Many authors believe that it contains numerous tubercle bacilli, but microscopically it is difficult to demonstrate. Why? Chemical composition of caseous necrosis is another unsolved problem. It is cheesy as because it contains excess cholesterol. But does it also contain protein or carbohydrate? This descriptive study was performed to explore the morphological findings, bacteriological features and biochemical composition of caseous material obtained from tubercular lymph nodes. The findings are interesting and may be valuable to medical professionals engaged in pathology, microbiology, biochemistry, ph...

Bangladesh Journal of Infectious Diseases

Background: Tuberculous lymphadenitis is common manifestation of extra-pulmonary tuberculosis in ... more Background: Tuberculous lymphadenitis is common manifestation of extra-pulmonary tuberculosis in Bangladesh. Objective: The purpose of the present study was to see the histopathological features of tuberculous lymphadenitis patients. Methodology: This cross sectional study was done at the Department of Pathology at Banghabandhu Sheikh Mujib Medical University (BSMMU), Dhaka from January 2009 to March 2011 for a period of nearly two years. All the patients irrespective of age and sex with the clinical features suggestive of tuberculous lymphadenitis and later on proved to be the same histologically were selected for the study purposively. Relevant information was recorded in a prescribed data sheet and histomorphological findings were recorded accordingly. In cases where fresh specimen was available, caseous portion of lymph node was sent for culture. FiteFaraco staining was also done on lymph node sections in all cases. Result: A total number of 50 tuberculous lymphadenitis patients were recruited for this study of which well formed granuloma was detected in 34(68.0%) cases followed by ill defined and combination of well and ill defined which were 8(16.0%) cases in each. Among 34 well defined cases coalescing and discrete were found in 6(17.6%) cases in each. However, both types were found in 22(64.7%) cases.Conclusion: In conclusion well formed granuloma is most commonly found in tuberculous lymphadenitis with combined variety of coalescing and discrete histopathologic features.

Journal of Tuberculosis Research, 2016

Background: Clinical and demographic features are important for the tuberculous lymphadenitis pat... more Background: Clinical and demographic features are important for the tuberculous lymphadenitis patients for treatment. Objective: The purpose of the present study was to see the clinical and demographic features of tuberculous lymphadenitis patients. Methodology: This cross-sectional study was done at the Department of Pathology at Banghabandhu Sheikh Mujib Medical University (BSMMU), Dhaka from January 2009 to March 2011 for a period of nearly two years. All the patients, irrespective of age and sex with the clinical features, suggestive of tuberculous lymphadenitis and later on, proved to be the same histologically, were selected for the study purposively. Relevant information was recorded in a prescribed data sheet and histomorphological findings were recorded accordingly. In cases where fresh specimen was available, caseous portion of lymph node was sent for culture. Fite Faraco staining was also done on lymph node sections in all cases. Result: A total number of 50 tuberculous lymphadenitis patients were recruited for this study. Among this study population, the age of cases ranged from 12 years to 65 years with a mean age of 26.22 ± 2.52 years. Male and female ratio was 1:1.38. Besides lymphadenopathy, 45 patients had low grade fever; 32 patients had weight loss and 18 patients had cough. Among 50 cases of lymph node tuberculosis cervical lymphadenopathy was 41 (82.0%) cases; 6 (12.0%) cases had supraclavicular lymphadenopathy; 2 (4.0%) cases had inguinal lymphadenopathy and 1 (2.0%) had axillary lymphadenopathy. The mean duration of presentation of lymphadenopathy with standard deviation was 7.06 ± 1.6 months.

Background: Now a day's determination of estrogen receptor (ER), progesterone receptor (PR) and H... more Background: Now a day's determination of estrogen receptor (ER), progesterone receptor (PR) and HER-2/neu expression pattern by immunohistochemistry in invasive breast cancer have become the standard procedure for breast cancer management. Objective: To see the expression pattern of estrogen receptor, progesterone receptor and HER-2/neu in Bangladeshi women with invasive breast carcinoma. Method: This cross sectional study was performed in 87 cases of invasive breast cancer. Estrogen receptor (ER), Progesterone receptor (PR) and HER-2/neu expression pattern were assessed by immunohistochemistry using monoclonal antibodies for detecting estrogen and progesterone receptors, and polyclonal antibody for detecting HER-2/neu. Results: All the cases were graded according to Bloom-Richardson grading system. Of those, Grade I tumour was 18 (20.69%), Grade II tumour was 58 (66.67%) and Grade III tumour was 11(12.64%). Both ER and PR positive reactivity were same and it was found 65 (74.71%) and HER-2/neu reactivity pattern were found negative in 59 (67.82%) cases and positive in 28 (32.18%) cases. A statistically significant correlation was found between the expression of ER and low grade tumour (p=0.011) and combined estrogen and progesterone receptor positive reactivity with low grade tumour (p=0.002). Conclusion: ER, PR and HER-2/neu expression do not correlated with each other, so it is recommended that each test should be independently determined by immunohistochemistry in all cases of invasive breast cancer. All equivocal cases of HER-2/neu (score 2+) should be analyzed by FISH technique to find out the percentage of real score.

Leukemia & …, 2000

The effect of molecular factors in the outcome of Hodgkin's Disease (HD) is being currently ... more The effect of molecular factors in the outcome of Hodgkin's Disease (HD) is being currently studied. In a previous series of HD. including patients treated only with radiotherapy and patients treated with chemotherapy (with or without radiotherapy). we found that a high ...

Bangladesh Journal of Otorhinolaryngology, 2012

Bangladesh Journal of Otorhinolaryngology, 2011

Introduction: Thyroglossal cyst is the most common congenital midline neck swelling. Most of the ... more Introduction: Thyroglossal cyst is the most common congenital midline neck swelling. Most of the cases are benign with rare malignant transformation is approximately in 1-2% cases. Only about 200 cases have been reported from different centers since 1911. Clinical presentation is similar to that of benign cyst and diagnosis is usually made incidentally during histopathological examination of excised cyst. Case report: We report a case of 46-year-old woman who underwent Sistrunk's operation for thyroglossal cyst. Histopathological examination revealed papillary carcinoma. Thyroxine was added in suppressive dose and patient was on regular follow-up for 2 years. No thyroid abnormality was found preoperative cytology, scintigraphy as well as per-operative procedure and post-operative follow-up period.

Bangladesh Journal of Pathology, 2009

Maxillofacial surgeons often deal with many cases of delayed wound healing, some are related to k... more Maxillofacial surgeons often deal with many cases of delayed wound healing, some are related to known risk factors and some are unknown. This study was aimed to assess the histological features of wound healing on day 0 and day 7 in postoperative cases of maxillofacial region in patients with or without risk factors. Microscopic examination of tissue specimen is a reliable and reasonably safe method to evaluate the histological differences. Six known risk factors for delayed wound healing were studied, which are commonly associated with maxillofacial pathology. Both clinical and histological examinations were performed for the evaluation of 32 postoperative cases. Out of 32 patients, 17 patients with risk factor were in study group and 15 apparently healthy persons without risk factor were in control group. Postoperative clinical evaluation of wound was done at 3 rd , 5 th , 7 th , 9 th , and 11 th day and histological examination of tissue specimen was done on day 0 and day 7. Based on six histological parameters, microscopic examination of tissue specimen was done by both routine Haematoxylin and Eosin stain and Masson's Trichrome stain. In day 7, healing wound of study group having risk factors showed profound amount of granulation tissue, early collagen fibres, plenty inflammatory infiltrate, vertical orientation of reticular pattern of collagen and minimum amount of mature collagen in majority of patients which indicates delayed healing. On the other hand, in control group majority showed horizontally oriented mature collagen fibres in fascicle. Statistically, significant association was found between study and control subjects in terms of pattern of collagen tissue, amount of early and mature collagen tissue at the 7 th day of follow up. Clinical evaluation also had strong association with histological state of healing.

Bangabandhu Sheikh Mujib Medical University Journal, 2012

Case Reports in Neurological Medicine, 2016

Background. Rosai-Dorfman disease (RDD) is a benign histiocytic proliferative disorder of unknown... more Background. Rosai-Dorfman disease (RDD) is a benign histiocytic proliferative disorder of unknown etiology. This rare condition commonly causes massive cervical lymphadenopathy. Intracranial RDD without any nodal involvement is extremely rare. Case Report. A young Bangladeshi male complained of bilateral complete blindness with left sided deafness for about three years. There was no lymphadenopathy. MRI and CT scan of brain suggested an inflammatory/neoplastic (?meningioma) lesion located at left parasellar region which extended frontally to encircle both optic nerves and also to left prepontine area. Histopathologically the lesion was diagnosed as RDD. The patient was treated with steroid and significant clinical improvement observed. Conclusion. The prognosis of intracranial RDD is not poor. It can be treated with surgery with or without corticosteroids, chemotherapy, and so forth. But as the condition is extremely rare and often misdiagnosed, the clinician, radiologist, and histopathologist should have a suspicion in their mind about the possibility of RDD.

Scholars Journal of Applied Medical Sciences

Introduction: Lymphoma, a type of cancer affecting the lymphatic system, requires accurate diagno... more Introduction: Lymphoma, a type of cancer affecting the lymphatic system, requires accurate diagnostic methods for effective treatment. This study aimed to evaluate the validity of Cell Block Histopathology and Fine Needle Aspiration Cytology (FNAC) in the diagnosis of lymphoma. Methods: A total of 30 patients with clinically suspected lymphoproliferative disorder were included in the study. Both Cell Block Histopathology and FNAC were used to diagnose lymphoma in these patients. The results were then compared with the final diagnosis based on histopathology, immunochemistry, and clinical follow-up, which was considered the gold standard. Result: The study included 30 patients with clinically suspected lymphoproliferative disorder. The Cell Block Histopathology method diagnosed 12 patients (40%) as lymphoma positive, 11 patients (36.67%) as lymphoma negative, and 7 cases (23.33%) were inconclusive. The Fine Needle Aspiration Cytology (FNAC) method diagnosed 13 patients (43.33%) as ly...

Bangladesh Journal of Medical Science

Sclerosing stromal tumour (SST) is a rare benign sex cord stromal tumour occurring in women in th... more Sclerosing stromal tumour (SST) is a rare benign sex cord stromal tumour occurring in women in their second and third decades. Patients usually present with menstrual irregularity and pelvic pain. Microscopically, this tumour is characterized by epithelioid and spindle cells arranged in pseudolobules separated by areas with fibrous deposition of various amount. Presence of ‘staghorn like’ proliferating vasculature is the hallmark feature of this tumour. The main differential diagnoses are thecoma and fibroma. Immunohistochemistry can be used to differentiate these tumours. This relatively new entity should be kept in mind while reporting ovarian tumour in a young female. We have described SST in an 18 year old female in this case report. Bangladesh Journal of Medical Science Vol. 22 No. 03 July’23 Page : 712-717

Bangabandhu Sheikh Mujib Medical University Journal

Epstein Barr virus plays an important role in the pathogenesis of Hodgkin lymphoma. However, the ... more Epstein Barr virus plays an important role in the pathogenesis of Hodgkin lymphoma. However, the frequency of its association in Bangladeshi people has not been widely studied. The aim of this study was to determine the association of Epstein Barr virus association in Hodgkin Lymphoma through detection of Latent Membrane Protein 1 by immunohistochemistry and serum Epstein Barr virus viral capsid antigen IgG antibody titer by serology. This was a cross-sectional study in purposively selected 45 histologically diagnosed cases of Hodgkin lymphoma at Bangabandhu Sheikh Mujib Medical University, Dhaka, during the period of March 2017 to December 2018. Latent membrane protein 1 was positive in 71.1% of cases of Hodgkin lymphoma. Among these positive cases, 96% of cases had significantly raised titer of serum Epstein Barr virus viral capsid antigen IgG antibody (P<0.0001 obtained by Fisher’s Exact test), which had a male predominance. Mixed cellularity classical Hodgkin lymphoma showed ...

Neurology India, 2011

Rosai Dorfman disease (RDD), also known as sinus histiocytosis with massive lymphadenopathy, is a... more Rosai Dorfman disease (RDD), also known as sinus histiocytosis with massive lymphadenopathy, is a benign histiocytic proliferative disorder mainly affecting the lymph nodes. Although several cases of extra-nodal involvement have been reported previously, central nervous system involvement, particularly in the absence of nodal disease is extremely rare. We report a case of isolated intracranial RDD occurring in a relatively elder patient, which was shown by histological examination to have a dura-based involvement.

SAGE Open Medicine, 2021

Objectives: Epstein–Barr virus is a tumorigenic virus and has been extensively studied as a causa... more Objectives: Epstein–Barr virus is a tumorigenic virus and has been extensively studied as a causative agent for Hodgkin lymphoma. Although immunostaining of the tumor biopsy is the standard method for diagnosis of Epstein–Barr virus-driven Hodgkin lymphoma, the invasiveness of the procedure renders it difficult and less desirable for the patients. Therefore, we designed this study to evaluate the efficiency of plasma Epstein–Barr virus DNA detection as an alternative diagnostic and prognostic method for Epstein–Barr virus–associated Hodgkin lymphoma. Methods: This analytical cross-sectional study was conducted during March 2017 to December 2018 including 43 Hodgkin lymphoma patients diagnosed histopathologically followed by the latent membrane protein-1 immunohistochemistry to determine their Epstein–Barr virus association. Plasma Epstein–Barr virus DNA in these samples was measured using quantitative polymerase chain reaction (qPCR). Results: Of total, 29 (67.44%) patients tested p...

SAS Journal of Medicine, 2021

Introduction: Breast cancer is the most commonly happening female cancer and the leading cause of... more Introduction: Breast cancer is the most commonly happening female cancer and the leading cause of cancer deaths worldwide. Breast cancer is a heterogeneous disease and it encompasses a variety of entities with distinct morphological appearances and clinical behaviors. Triple-negative breast cancer (TNBC) represents 12.5– 15% of all breast cancers. Its molecular dissimilarity also brings a significantly different clinical course. It has been observed that basal-like breast cancers exhibit a gene expression profile very similar to that of myoepithelial cells. Aim of the study: To identify the basal like TNBC cases and correlate them with histomorphological grade. Material & Methods: This cross sectional study was conducted in the department of BSMMU from January 2016 to December 2017. A total of 41 cases previously diagnosed as TNBC by histological and immunohistochemical examinations were selected for the study. During collecting specimen, all relevant information were recorded syste...

Bangladesh Journal of Obstetrics & Gynaecology

Objective of the study: The increasing inter- and intra-observer variability in the diagnosis of ... more Objective of the study: The increasing inter- and intra-observer variability in the diagnosis of CIN by histopathology has led to the advent of different biomarkers. The aim of this study is to evaluate Ki-67 and p16 INK4a marker in differentiating CIN lesions and the clinical utility of it in the management of high grade squamous intraepithelial lesions (HSIL). Materials and methods: This cross-sectional study has been carried out among the biopsy proved cases of CIN2 and CIN3 in which P16 and Ki-67 immunostain was performed in the Colposcopy clinic, Bangabandhu Sheikh Mujib Medical University (BSMMU) and Department of Pathology, BSMMU respectively from 14-3-17 to 30-05-18. Thirty-two cases were incorporated in the study by purposive sampling. Data was analyzed using SPSS version 17. Results: Among 32 cases of HSIL, 25 were diagnosed as CIN 2 (78.13 %) and 7 cases as CIN 3 (21.87 %) by histopathology. All the cases were subjected to immunohistochemistry with P16 and Ki 67. Regardin...

Journal of Current and Advance Medical Research, 2016

Background: Colorectal carcinoma is one of the most common human malignancies worldwide. Objectiv... more Background: Colorectal carcinoma is one of the most common human malignancies worldwide. Objectives: The purpose of the present study was to see the clinico-demographic Characteristics of colorectal carcinoma. Methodology: This cross sectional study was carried out in the Department of Pathology at Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh during the period of November 2009 to July 2010. Patients with colorectal carcinoma from all ages and both sexes were included in the study. Detailed clinical information was obtained by taking history from patient's attendant's statement. Fresh unfixed specimens were obtained after surgical resection. Result: A total of 50 cases of colorectal adenocarcinoma from all ages and both sexes were included in the study. The mean age of the 50 cases was 47±14.8 years. Per rectal bleeding was the most common clinical presentation of colorectal carcinoma which was 20(40.0%) cases followed by abdominal pain, altered bowel habit, generalized weakness, anorexia and pallor and p/r bleeding and abdominal pain which were 8(16.0%) cases, 7(14.0%) cases, 6(12.0%) cases and 6(12.0%) cases respectively. Conclusion: In conclusion middle age male patients are the most vulnerable for colorectal carcinoma with common presenting features of per rectal bleeding, abdominal pain and altered bowel habit.

Introduction: Thyroglossal cyst is the most common congenital midline neck swelling. Most of the ... more Introduction: Thyroglossal cyst is the most common congenital midline neck swelling. Most of the cases are benign with rare malignant transformation is approximately in 1-2% cases. Only about 200 cases have been reported from different centers since 1911. Clinical presentation is similar to that of benign cyst and diagnosis is usually made incidentally during histopathological examination of excised cyst. Case report: We report a case of 46-year-old woman who underwent Sistrunk’s operation for thyroglossal cyst. Histopathological examination revealed papillary carcinoma. Thyroxine was added in suppressive dose and patient was on regular follow-up for 2 years. No thyroid abnormality was found preoperative cytology, scintigraphy as well as per-operative procedure and post-operative follow-up period. Conclusion: Sistrunk’s operation followed by suppressive dose of thyroxin and regular follow up is recommended for thyroglossal duct cyst carcinoma in asymptomatic incidental case without ...

Ovarian cancer is a devastating disease preoperative evaluation of the patients with an ovarian t... more Ovarian cancer is a devastating disease preoperative evaluation of the patients with an ovarian tumour is difficult. So frozen section biopsy of ovarian tumour is important to determine the extent of surgery. This retrospective cross-sectional study was carried out in the Department of Gynaecological Oncology, Bangabandhu Sheikh Mujib Medical University from August 2016 to July 2017 to determine the validity of frozen section biopsy in the diagnosis of ovarian tumour. Fifty cases of ovarian tumour underwent frozen section biopsy were included by purposive sampling. Histopathological finding was taken as the gold standard. Data was analyzed by SPSS version 16. The sensitivity of frozen section in the diagnosis of benign, borderline and malignant were 100%, 100% and 97.67% respectively as well as the specificities were 100%, 97.96% and 100% respectively. Similarly the accuracy, PPV, NPV for the benign, borderline and malignant ovarian tumour were also high except the borderline tumour...

Tuberculosis is a major public health problem in Bangladesh. Caseous necrosis is the key histomor... more Tuberculosis is a major public health problem in Bangladesh. Caseous necrosis is the key histomorphological feature for the diagnosis of tuberculosis. This cheesy material is described microscopically as amorphous granular debris. But it has a number of variations in color, appearance, inflammatory cellular content, cellular debris and apoptotic bodies. Does it contain Mycobacteria? Many authors believe that it contains numerous tubercle bacilli, but microscopically it is difficult to demonstrate. Why? Chemical composition of caseous necrosis is another unsolved problem. It is cheesy as because it contains excess cholesterol. But does it also contain protein or carbohydrate? This descriptive study was performed to explore the morphological findings, bacteriological features and biochemical composition of caseous material obtained from tubercular lymph nodes. The findings are interesting and may be valuable to medical professionals engaged in pathology, microbiology, biochemistry, ph...

Bangladesh Journal of Infectious Diseases

Background: Tuberculous lymphadenitis is common manifestation of extra-pulmonary tuberculosis in ... more Background: Tuberculous lymphadenitis is common manifestation of extra-pulmonary tuberculosis in Bangladesh. Objective: The purpose of the present study was to see the histopathological features of tuberculous lymphadenitis patients. Methodology: This cross sectional study was done at the Department of Pathology at Banghabandhu Sheikh Mujib Medical University (BSMMU), Dhaka from January 2009 to March 2011 for a period of nearly two years. All the patients irrespective of age and sex with the clinical features suggestive of tuberculous lymphadenitis and later on proved to be the same histologically were selected for the study purposively. Relevant information was recorded in a prescribed data sheet and histomorphological findings were recorded accordingly. In cases where fresh specimen was available, caseous portion of lymph node was sent for culture. FiteFaraco staining was also done on lymph node sections in all cases. Result: A total number of 50 tuberculous lymphadenitis patients were recruited for this study of which well formed granuloma was detected in 34(68.0%) cases followed by ill defined and combination of well and ill defined which were 8(16.0%) cases in each. Among 34 well defined cases coalescing and discrete were found in 6(17.6%) cases in each. However, both types were found in 22(64.7%) cases.Conclusion: In conclusion well formed granuloma is most commonly found in tuberculous lymphadenitis with combined variety of coalescing and discrete histopathologic features.

Journal of Tuberculosis Research, 2016

Background: Clinical and demographic features are important for the tuberculous lymphadenitis pat... more Background: Clinical and demographic features are important for the tuberculous lymphadenitis patients for treatment. Objective: The purpose of the present study was to see the clinical and demographic features of tuberculous lymphadenitis patients. Methodology: This cross-sectional study was done at the Department of Pathology at Banghabandhu Sheikh Mujib Medical University (BSMMU), Dhaka from January 2009 to March 2011 for a period of nearly two years. All the patients, irrespective of age and sex with the clinical features, suggestive of tuberculous lymphadenitis and later on, proved to be the same histologically, were selected for the study purposively. Relevant information was recorded in a prescribed data sheet and histomorphological findings were recorded accordingly. In cases where fresh specimen was available, caseous portion of lymph node was sent for culture. Fite Faraco staining was also done on lymph node sections in all cases. Result: A total number of 50 tuberculous lymphadenitis patients were recruited for this study. Among this study population, the age of cases ranged from 12 years to 65 years with a mean age of 26.22 ± 2.52 years. Male and female ratio was 1:1.38. Besides lymphadenopathy, 45 patients had low grade fever; 32 patients had weight loss and 18 patients had cough. Among 50 cases of lymph node tuberculosis cervical lymphadenopathy was 41 (82.0%) cases; 6 (12.0%) cases had supraclavicular lymphadenopathy; 2 (4.0%) cases had inguinal lymphadenopathy and 1 (2.0%) had axillary lymphadenopathy. The mean duration of presentation of lymphadenopathy with standard deviation was 7.06 ± 1.6 months.

Background: Now a day's determination of estrogen receptor (ER), progesterone receptor (PR) and H... more Background: Now a day's determination of estrogen receptor (ER), progesterone receptor (PR) and HER-2/neu expression pattern by immunohistochemistry in invasive breast cancer have become the standard procedure for breast cancer management. Objective: To see the expression pattern of estrogen receptor, progesterone receptor and HER-2/neu in Bangladeshi women with invasive breast carcinoma. Method: This cross sectional study was performed in 87 cases of invasive breast cancer. Estrogen receptor (ER), Progesterone receptor (PR) and HER-2/neu expression pattern were assessed by immunohistochemistry using monoclonal antibodies for detecting estrogen and progesterone receptors, and polyclonal antibody for detecting HER-2/neu. Results: All the cases were graded according to Bloom-Richardson grading system. Of those, Grade I tumour was 18 (20.69%), Grade II tumour was 58 (66.67%) and Grade III tumour was 11(12.64%). Both ER and PR positive reactivity were same and it was found 65 (74.71%) and HER-2/neu reactivity pattern were found negative in 59 (67.82%) cases and positive in 28 (32.18%) cases. A statistically significant correlation was found between the expression of ER and low grade tumour (p=0.011) and combined estrogen and progesterone receptor positive reactivity with low grade tumour (p=0.002). Conclusion: ER, PR and HER-2/neu expression do not correlated with each other, so it is recommended that each test should be independently determined by immunohistochemistry in all cases of invasive breast cancer. All equivocal cases of HER-2/neu (score 2+) should be analyzed by FISH technique to find out the percentage of real score.

Leukemia & …, 2000

The effect of molecular factors in the outcome of Hodgkin's Disease (HD) is being currently ... more The effect of molecular factors in the outcome of Hodgkin's Disease (HD) is being currently studied. In a previous series of HD. including patients treated only with radiotherapy and patients treated with chemotherapy (with or without radiotherapy). we found that a high ...

Bangladesh Journal of Otorhinolaryngology, 2012

Bangladesh Journal of Otorhinolaryngology, 2011

Introduction: Thyroglossal cyst is the most common congenital midline neck swelling. Most of the ... more Introduction: Thyroglossal cyst is the most common congenital midline neck swelling. Most of the cases are benign with rare malignant transformation is approximately in 1-2% cases. Only about 200 cases have been reported from different centers since 1911. Clinical presentation is similar to that of benign cyst and diagnosis is usually made incidentally during histopathological examination of excised cyst. Case report: We report a case of 46-year-old woman who underwent Sistrunk's operation for thyroglossal cyst. Histopathological examination revealed papillary carcinoma. Thyroxine was added in suppressive dose and patient was on regular follow-up for 2 years. No thyroid abnormality was found preoperative cytology, scintigraphy as well as per-operative procedure and post-operative follow-up period.

Bangladesh Journal of Pathology, 2009

Maxillofacial surgeons often deal with many cases of delayed wound healing, some are related to k... more Maxillofacial surgeons often deal with many cases of delayed wound healing, some are related to known risk factors and some are unknown. This study was aimed to assess the histological features of wound healing on day 0 and day 7 in postoperative cases of maxillofacial region in patients with or without risk factors. Microscopic examination of tissue specimen is a reliable and reasonably safe method to evaluate the histological differences. Six known risk factors for delayed wound healing were studied, which are commonly associated with maxillofacial pathology. Both clinical and histological examinations were performed for the evaluation of 32 postoperative cases. Out of 32 patients, 17 patients with risk factor were in study group and 15 apparently healthy persons without risk factor were in control group. Postoperative clinical evaluation of wound was done at 3 rd , 5 th , 7 th , 9 th , and 11 th day and histological examination of tissue specimen was done on day 0 and day 7. Based on six histological parameters, microscopic examination of tissue specimen was done by both routine Haematoxylin and Eosin stain and Masson's Trichrome stain. In day 7, healing wound of study group having risk factors showed profound amount of granulation tissue, early collagen fibres, plenty inflammatory infiltrate, vertical orientation of reticular pattern of collagen and minimum amount of mature collagen in majority of patients which indicates delayed healing. On the other hand, in control group majority showed horizontally oriented mature collagen fibres in fascicle. Statistically, significant association was found between study and control subjects in terms of pattern of collagen tissue, amount of early and mature collagen tissue at the 7 th day of follow up. Clinical evaluation also had strong association with histological state of healing.

Bangabandhu Sheikh Mujib Medical University Journal, 2012

Case Reports in Neurological Medicine, 2016

Background. Rosai-Dorfman disease (RDD) is a benign histiocytic proliferative disorder of unknown... more Background. Rosai-Dorfman disease (RDD) is a benign histiocytic proliferative disorder of unknown etiology. This rare condition commonly causes massive cervical lymphadenopathy. Intracranial RDD without any nodal involvement is extremely rare. Case Report. A young Bangladeshi male complained of bilateral complete blindness with left sided deafness for about three years. There was no lymphadenopathy. MRI and CT scan of brain suggested an inflammatory/neoplastic (?meningioma) lesion located at left parasellar region which extended frontally to encircle both optic nerves and also to left prepontine area. Histopathologically the lesion was diagnosed as RDD. The patient was treated with steroid and significant clinical improvement observed. Conclusion. The prognosis of intracranial RDD is not poor. It can be treated with surgery with or without corticosteroids, chemotherapy, and so forth. But as the condition is extremely rare and often misdiagnosed, the clinician, radiologist, and histopathologist should have a suspicion in their mind about the possibility of RDD.