Figen Kaymaz - Academia.edu (original) (raw)

Papers by Figen Kaymaz

Okajimas Folia Anatomica Japonica, 1999

Türkiye klinikleri tıp bilimleri dergisi, 2013

Purkinje cell network of sheep ventricles as the terminal pathway of conduction system.

12th European Congress of Endocrinology, Apr 1, 2010

Archives of Orthopaedic and Trauma Surgery, Sep 18, 2020

Introduction A recent histopathological and immunohistochemical study has proved that the additio... more Introduction A recent histopathological and immunohistochemical study has proved that the addition of concentrated growth factors (CGF) to the Masquelet’s technique contributes to the quality of the membrane formed, in respect of inducing inflammation and proliferation, maintaining vascularization on large diaphyseal bone defects, and increasing the number of stem cells. The aim of the study is comparison of radiological results of this combination treatment by micro-CT. Materials and methods The study was planned on a critical bone defect model in rabbit radius. Group I and Group III were the control groups to which only the Masquelet’s technique is applied. Group II and Group IV were CGF groups in addition to the Masquelet’s technique. CGF was prepared by centrifugation of rabbit’s own blood. For early phase, Groups I and II were evaluated in the 8th week, while for late phase, Group III and Group IV were evaluated in the 12th week. Groups were compared in terms of bony union radiologically by micro-CT(μCT) (New Bone Volume (NBV), Total Bone Volume (TBV) and NBV/TBV) and histopathologically. Results The structural parameters, including NBV, TBV, NBV/TBV were higher in the early- (8th week) and late-phase (12th week) CGF group. There was no statistically significant difference between CGF and control groups in early phase, ( p = 0.153), while in late phase, CGF group was significantly higher of new bone volume than the control group, 246.3 mm 3 (196.1–258) and 169.6 mm 3 (154.3–235.9), respectively ( p = 0.028). For early phase, control group was significantly lower than late-phase control group, 121.8 mm 3 (88.8–144.4) and 169.6 mm3 (154.3–235.9), respectively ( p = 0.006). The ratio of New Bone Volume to Total Bone Volume ( NBV/TBV ratio) in CGF groups was significantly higher compared to the control groups 27.3% (24.7–29.6), 35.3% (32.1–38.6) ( p = 0.032) and 39.7% (36.7–41.6), 55.3% (52–57.5) ( p = 0.002), respectively. Histopathologically, Microscopic New Bone Formation had no statistically significant difference between control and CGF groups in early phase (8th week) ( p = 0.153), while in late phase (12th week), CGF group had significantly higher amount of new bone formation than the control group, 0.29 µm 2 (0.27–0.36), 0.51 µm 2 (0.42–0.59), respectively ( p = 0.008). Conclusion The addition of CGF to the Masquelet’s technique is an important method for supporting new bone formation in large diaphyseal bone defects. Level evidence Level III, therapeutic/care management.

Histochemistry and Cell Biology, Aug 31, 2011

Dental pulp stem cells (hDP-SCs) were primarily derived from pulp tissues of primary incisors, ex... more Dental pulp stem cells (hDP-SCs) were primarily derived from pulp tissues of primary incisors, exfoliated deciduous and permanent third molar teeth. To understand the characteristics of hDP-SCs from impacted third molar, proliferation capacities, gene expression profiles, phenotypic, ultrastructural, and differentiation characteristics were analyzed in comparison with human bone marrow-derived mesenchymal stem cells (hBM-MSCs), extensively. hDP-SCs showed more developed and metabolically active cells. Contrary to hBM-MSCs, hDP-SCs strongly expressed both cytokeratin (CK)-18 and -19, which could involve in odontoblast differentiation and dentine repair. The intrinsic neuro-glia characteristics of hDP-MSCs were demonstrated by the expression of several specific transcripts and proteins of neural stem cell and neurons. These cells not only differentiate into adipogenic, osteogenic, and chondrogenic lineage, but also share some special characteristics of expressing some neural stem cell and epithelial markers. Under defined conditions, hDP-SCs are able to differentiate into both neural and vascular endothelial cells in vitro. Dental pulp might provide an alternative source for human MSCs. hDP-SCs with a promising differentiation capacity could be easily isolated, and possible clinical use could be developed for neurodegenerative and oral diseases in the future.

Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology, Feb 1, 2000

Talon cusp is a tooth anomaly that affects both the primary and the permanent dentitions. However... more Talon cusp is a tooth anomaly that affects both the primary and the permanent dentitions. However, the occurrence of this anomalous cusp is rather infrequent in the primary dentition. Only 7 cases of bilateral talon cusps affecting the primary teeth have been reported in the dental literature. This is a case report of bilateral talon cusps on primary maxillary central incisors whose histologic evaluation revealed the existence of pulpal tissue in the anomalous cusps.

Archives of basic and clinical research, Jun 22, 2020

Turkiye Klinikleri Tip Bilimleri Dergisi, 2008

Yasal uyarı: Bu sitede yayınlanan resim, yazı ve diğer uygulamaların her hakkı Ortadoğu Reklam Ta... more Yasal uyarı: Bu sitede yayınlanan resim, yazı ve diğer uygulamaların her hakkı Ortadoğu Reklam Tanıtım Yayıncılık Turizm Eğitim İnşaat Sanayi ve Ticaret A.Ş. 'ye aittir. Kaynak gösterilmeden kullanılamaz. Bu site hekimleri sağlık alanında bilgilendirmeye yönelik hazırlanmıştır. ...

Journal of the Turkish-German Gynecological Association, Mar 1, 2018

Objective: To compare the degree of apoptosis in ovaries and tubal epithelium observed secondary ... more Objective: To compare the degree of apoptosis in ovaries and tubal epithelium observed secondary to tubal ligation either by Pomeroy's method or bipolar electrocauterization in a rat model. Material and Methods: A total of 24 female Sprague-Dawley rats were randomly assigned into 3 study groups: control (n=8), Pomeroy (n=8), and the electrocauterization group (n=8). Apoptotic cells were detected on the primary, secondary, tertiary follicles of the ovaries, and on the tubal epithelium using terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end-labeling. The apoptotic index was calculated for each group by the percentage of the stained cells. Results: The apoptotic index of tubal epithelium was significantly higher in the bipolar electrocauterization group compared with the control and Pomeroy groups (3.1±0.8 vs. 1.4±1.0, p=0.018 and 2.0±1.2, p=0.03, respectively) whereas there was no significant difference between Pomeroy's method and the control group. The apoptotic index of primary follicles was higher in the bipolar electrocauterization group compared with the control and Pomeroy's method groups (3.4±0.5 vs. 1.2±0.4, p<0.001 and 1.8±0.8, p=0.005, respectively), but there was no significant difference between Pomeroy's method and the control group. The apoptotic index of secondary and tertiary follicles was similar for each group. Conclusion: Pomeroy's technique, as a permanent contraception method, is associated with lower apoptotic index on ovary and fallopian tube when compared with bipolar electrocauterization.

European Archives of Oto-rhino-laryngology, Oct 25, 2006

Anticancer agents may cause side effects and some of which may be dose dependant. It is important... more Anticancer agents may cause side effects and some of which may be dose dependant. It is important for clinicians to see the effects on tissues histopathologically. The aim of this study was to investigate the effects of topotecan (hycamtin), a topoisomerase I inhibiting anticancer agent, on nasal, buccal, and lingual mucosa of rabbits. The study was carried out in two groups each consisting of 20 rabbits. Rabbits in group I received i.v. topotecan (0.5 mg/kg once daily) for 3 days. Rabbits in group II received i.v. topotecan (0.25 mg/kg once daily) for 3 days. In group I and II, biopsies from the nasal, buccal, and lingual mucosa were taken on the fourth (1 day after the 3-day topotecan treatment) and 15th day (12 days after the 3-day topotecan treatment). Light and transmission electron microscopic (TEM) observations have shown that nasal mucosa was not affected by topotecan administration. Topotecan treatment resulted in the formation of some ulcerative lesions in the lingual mucosa especially on the lower surface of the tongue. On the dorsal surface, the epithelium showed highly edematous and degenerating cells and separations in the stratum granulosum. In the buccal mucosa, effects were similar. In lingual and buccal mucosa, healing was observed on the 15th day. The oral (lingual and buccal) mucosal side effects of topotecan were observed as reversible and not dose dependent. It was concluded that these side effects are not severe, and topotecan may be used safely in cancer treatment.

Knee Surgery, Sports Traumatology, Arthroscopy, Feb 8, 2006

The objective of this study was to evaluate the effect of subcutaneously injected nicotine on tra... more The objective of this study was to evaluate the effect of subcutaneously injected nicotine on transversely transected and sutured achilles tendon healing in an experimental rabbit model. Adult New Zealand rabbits (n=22) weighting 3,000-3,500 g were used in this experimental study. Rabbits were randomly divided into two groups. Achilles tendon was transversely incised and repaired in all animals. In the experiment group subcutaneous injection of Nicotine tartrate 3 mg/kg/day was done. In the control group Serum physiologic injection was done at the same dosage. The injections were made three times a day in equal dosages. Nicotine and SF injections were made until the end of the 8-week, and then all animals were euthanized. Both light microscopic and electron microscopic evaluations were made on 14 animals. In N group light microscopic evaluation showed a visible gap in repair site. The total tendon score represented in N group was less than in SF group. The statistical analysis of the groups was significantly different for total tendon scores (P=0.002). Beside this electron microscopic examination showed inactive and degenerated fibroblasts and irregular collagen fibrils around them as well as collagen synthesis interruption in N group. Biomechanical evaluation was made on eight animals. The average tensile strength values in Group N (139.47+/-44.55 N) were significantly lower than those in Group SF (265.9+/-39.01 N) (z=2.309, P=0.029). Nicotine is the major chemical component common to all cigarettes and previously has been shown to affect wound and fracture healing adversely. The results of this study show that nicotine impairs achilles tendon healing after a surgical repair.

Uludağ Üniversitesi Tıp Fakültesi Dergisi

Tavuk koryoallantoik membran (CAM) modeli, anjiyogenez çalışmalarında gelişimsel süreçleri takip ... more Tavuk koryoallantoik membran (CAM) modeli, anjiyogenez çalışmalarında gelişimsel süreçleri takip etmek için yaygın olarak kullanılan bir modeldir. Bu çalışmada, CAM kullanılarak, embriyonik gelişimin 7. ve 20. günleri (E7 ve E20) arasında görülen anjiyogenez süreci ışık ve transmisyon elektron mikroskop analizleri ile takip edilmiştir. Ayrıca, aynı günlerde alınan CAM örnekleri kullanılarak, anjiyogenez meka-nizmalarında görevli [Vasküler Endotelyal Büyüme Faktörü (VEGF)-A ve Fibroblast Büyüme Faktörü (FGF)-2] ve reseptörlerinin [Vasküler Endotelyal Büyüme Faktörü Reseptörü (VEGFR)-2 ve Fibroblast Büyüme Faktörü (FGFR)-2] yanı sıra, insan C-tipi natriüretik peptid (CNP) homoloğu olan tavuk CNP-3 ve reseptörü natriüretik peptid reseptörü (NPR)-B'nin mRNA ifade düzeylerinin zamana bağımlı değişimi analiz edilmiştir. Işık mikroskobu ve elektron mikroskobu analizleri, endotel tüp benzeri vasküler yapıları yoğun olarak E7-E8 günlerinde ve koryonik mezenşim içinde bulunduğunu göstermi...

Hacettepe Üniversitesi Yayınları eBooks, 2016

Histoloji; Tıp eğitiminde insana ait birçok farklı hücre, doku ve organların yapılanmalarını araş... more Histoloji; Tıp eğitiminde insana ait birçok farklı hücre, doku ve organların yapılanmalarını araştıran, yorumlayan, fonksiyonel aktiviteleri ile ilişkilendiren ve mikroskobik görüntülenmelerini içeren önemli bir sağlık bilimidir. Bu kitap çeşitli hücre, doku ve organların histolojilerini ve bunlara ait mikrografları içermektedir. Kitabın amacı öğrencilerin laboratuvarda preparatları mikroskop altında incelerken geçirdikleri zamanı verimli kılmak ve bu sırada hücrenin biyolojik aktivitesine yönelik fonksiyonel yapılanmasını histokimyasal boyanma özellikleriyle ilişkilendirmelerini sağlamaktır. Kitaptaki her bölüm; mikrograflar ve kısa açıklamaları ile organ, doku ve hücrelerin temel özellikleri hakkında bilgi vermektedir. Preparatlardan alınan mikrograflar; Hacettepe Üniversitesi Tıp Fakültesi Histoloji ve Embriyoloji Anabilim Dalı; laboratuvar teknisyenleri, araştırma görevlileri, uzmanları ve akademik personelinin yıllar içinde katkıda bulunduğu emeğidir

Biotechnic & Histochemistry, 2017

We investigated the microscopic structure of transverse sections of the oculomotor, trochlear and... more We investigated the microscopic structure of transverse sections of the oculomotor, trochlear and abducens nerves of Arabian foals using stereological methods. Bilateral nerve pairs from 2month-old female Arabian foals were analyzed. The tissues were embedded in plastic blocks, then 1 µm thick sections were cut and stained with osmium tetroxide and methylene blue-azure II. Stereology was performed using light microscopy. Morphometry showed that the right and left pairs of nerves were similar. The transverse sectional areas of the oculomotor, trochlear and abducens nerves were 1.93 ± 0.19 mm 2 , 0.32 ± 0.06 mm 2 and 0.70 ± 0.08 mm 2 , respectively. The oculomotor nerve exhibited a significantly greater number of myelinated axons (16755 ± 1279) and trochlear (2656 ± 494) and the abducens nerves (4468 ± 447). The ratio of the axon diameter to myelinated nerve fiber diameter was 0.58, 0.55 and 0.55 for the oculomotor, trochlear and abducens nerves, respectively. Of the three nerves studied, the abducens nerve exhibited the greatest nerve fiber area, myelin area, nerve and axon diameters, and myelin thickness. The ratio of small myelinated nerve fibers was greatest in the oculomotor nerve.

Acta Histochemica, 2007

Some studies have documented taste changes in patients with diabetes mellitus (DM). In order to u... more Some studies have documented taste changes in patients with diabetes mellitus (DM). In order to understand the relationships between taste disorders caused by DM and the innervation and morphologic changes in the taste buds, we studied the vallate papillae and their taste buds in rats with DM. DM was induced in these rats with streptozotocin (STZ), which causes the death of b cells of the pancreas. The rats were sacrificed and the vallate papillae were dissected for morphometric and quantitative immunohistochemical analyses. The innervations of the vallate papillae and taste buds in diabetic and control rats were detected using immunohistochemistry employing antibodies directed against protein gene product 9.5 (PGP 9.5) and calcitonin gene-related peptide (CGRP). The results showed that PGP 9.5-and CGRPimmunoreactive nerve fibers in the trench wall of diabetic vallate papillae, as well as taste cells in the taste buds, gradually decreased both intragemmally and intergemmally. The morphometry revealed no significant difference in papilla size between the control and diabetic groups, but there were fewer taste buds per papilla (per animal). The quantification of innervation in taste buds of the diabetic rats supported the visual assessment of immunohistochemical labeling, that the innervation of taste cells was significantly reduced in diabetic animals. These findings suggest that taste impairment in diabetic subjects may be caused by neuropathy defects and/or morphological changes in the taste buds.

Acta Orthopaedica et Traumatologica Turcica, 2012

The aim of this study was to evaluate the effects of bone marrow-derived mesenchymal stem cell an... more The aim of this study was to evaluate the effects of bone marrow-derived mesenchymal stem cell and bone marrow elements on the healing of meniscal tears. Methods: This study was performed on twelve, 2-year-old male Tahirova sheep. In each subject, one knee was used for experiment purposes and the other knee was used as a control. After creating a longitudinal full-thickness tear in the red-white zone of the medial meniscus, aspirated autologous bone marrow material was injected into the tear site in the experiment group. The control group received no intervention for secondary healing. Results: In the macroscopic evaluation of meniscus, a bridging reparation tissue and adhesion were observed between the rims of the tear in the experiment group. There was no statistical difference in collagen fibril formation between the groups (p=0.16). There was significantly more neovascularization in the experiment group than the control group (p=0.003). The cell count was also a significantly higher in the experiment group (p=0.004) and formation of cartilage plaques was more frequent in the experiment group (p=0.016). There was no evidence suggesting intrinsic repair in the meniscus of control group by light and electron microscopy. Conclusion: An injection of bone marrow into the meniscus tear site improves healing in a meniscal tear model as demonstrated by both light and electron microscopic findings.

Sinir Sistemi Cerrahisi Dergisi, 2022

Amaç: Bu çalışma sıçanlarda iyonize radyasyon sonrası gelişen beyin ve spinal kord hasarı modelin... more Amaç: Bu çalışma sıçanlarda iyonize radyasyon sonrası gelişen beyin ve spinal kord hasarı modelinde deksametazon ve melatonin etkinliğini karşılaştırmak için yapılmıştır. Yöntem: Radyasyon verilen sıçanlarda tek doz 6.75 Gy (LD50) tüm vücut ışınlaması yapıldı. Toplamda 8 adet erkek Wistar albino sıçan rastgele olarak 4 gruba bölündü ve her bir grup 24 saatlik ve 72 saatlik takip edilecek şekilde 2 alt gruba bölündü: 1) Sham grupları (S24 ve S72), 2) Işınlama yapılan kontrol grupları (K24 ve K72), 3) Işınlama sonrası deksametazon (0,25 mg/kg) uygulanan gruplar (D24 ve D72) ve 4) Işınlama sonrası melatonin (10 mg/kg) uygulanan grupla (M24 ve M 72). Beyin ödemi, oksidatif stres (GSH ve MDA düzeyleri ölçülerek değerlendirildi) ve histopatolojik doku hasar skoru değerlendirildi. Bulgular: Sham grubu ile karşılaştırıldığında beyin ve sıvı içeriği kontrol gruplarında artarken deksametazon ve melatonin uygulanan gruplarda benzerlik gösteriyordu. Oksidatif stres, melatonin uygulaması ile aza...

Nephrology Dialysis Transplantation, 2017

renal ischemia reperfusion injury (IRI) related in vitro and in vivo models, which was reversed b... more renal ischemia reperfusion injury (IRI) related in vitro and in vivo models, which was reversed by a novel cyclic helix B peptide (CHBP), erythropoietin derivative. Synthetic small interfering RNA (siRNA) transiently silenced detrimental genes posttranscriptionally in renal IRI. Here, we further investigated the effects of modifying TRPM7 by siRNA or CHBP on its down stream function in TCMK-1 cells. METHODS: After TCMK-1 cells were transfected with synthetic TRPM7 siRNA using a cationic lipid-based transfection regent lipofectamine 2000, the expression of TRPM7 mRNA expression was detected to select the most effective sequence of TRPM7 siRNA. Using whole-cell patch-clamping, TRPM7-like currents were first recorded in mouse TCMK-1 cells, and then the regulation of TRPM7-like currents by TRPM7 siRNA or CHBP was also investigated. RESULTS: In the TCMK-1 cells transfected with TRPM7 siRNA with sequence number 3239, 1793 and 5326, the expression of TRPM7 mRNA was respectively reduced by 33%, 42% and 28% compared to the cells treated by the negative control siRNA for 24 h. In addition, inward and outward TRPM7 currents were time-dependently increased using pipettes filled with internal Mg 2+-free solution, and the TRPM7-like current was successfully identified in TCMK-1 cells. Furthermore, TRPM7 siRNA 1793 sequence at 40 nM for 48 h caused a noticeable reduction of outward TRPM7 current in comparison to cells treated with the negative control siRNA. In addition, CHBP treatment at 100 nmol/L also inhibited the TRPM7-like current in TCMK-1 cells. CONCLUSIONS: Synthetic siRNA targeting TRPM7 and CHBP both decreased TRPM7-like currents, suggesting a potential renoprotection that might associate with calcium overload inhabitation. However, the function and mechanism of TRPM7 in renal IRI, as well as the renoprotection of CHBP, are worthy to be further investigated.

Clinical and Experimental Obstetrics & Gynecology, 2015

Objective: To compare the percentage and ultrastructure of normal and abnormal granulosa cells an... more Objective: To compare the percentage and ultrastructure of normal and abnormal granulosa cells and their effect on fertilization and pregnancy rate between gonadotropin releasing hormone (GnRH) agonist and antagonist treatment. Materials and Methods: In this study, granulosa cells obtained from 22 women undergoing in vitro fertilization (IVF) treatment due to unexplained infertility with either with GnRH agonist (n=11) or GnRH antagonist (n=11) were evaluated by light and electron microscopy. Results: GnRH agonist and antagonist therapy was found to have no effect in terms of abnormal granulosa cell percentage (0.0679 ± 0.08977 vs 0.0481 ± 0.05164; p > 0.05), fertilization [85 (45-90) vs 75 (64-93)] and pregnancy rate (37% vs 46%). Light microscopic observations showed similar features of normal cells of agonist and antagonist-treated cells. Ultrastructural evaluation also revealed that there was no difference between cells of two treatment groups. Conclusion: Both GnRH agonist and antagonist treatment for ovarian stimulation may have similar effects on granulosa cells at the morphological and ultrastructural level, as well as on fertilization and pregnancy rates.