Fitnat Topbas - Academia.edu (original) (raw)

Papers by Fitnat Topbas

Research paper thumbnail of Single-layer versus double-layer closure of the vaginal cuff with barbed sutures in laparoscopic hysterectomy

Ginekologia Polska, 2018

Objectives: The utilization of barbed sutures in laparoscopic hysterectomy has become popular amo... more Objectives: The utilization of barbed sutures in laparoscopic hysterectomy has become popular among gynecologic surgeons. Our aim was to compare the outcomes of two different techniques for closing the vaginal cuff with barbed sutures in laparoscopic hysterectomies. Material and methods: A retrospective study was completed on 202 patients who underwent laparoscopic hysterectomy for benign diseases at Istanbul Kanuni Sultan Suleyman Training and Research Hospital from April 2014 through June 2016. In group 1 (n = 139), a single-layer continuous suturing method was used; each bite contained the pubocervical fascia and vaginal mucosa anteriorly, and vaginal mucosa and rectovaginal fascia posteriorly. In group 2 (n = 63), a double-layer continuous suturing method was used; only vaginal mucosa was included in the first layer, and a second layer incorporated the pubocervical and rectovaginal fascias. Results: Patient characteristics (age, body mass index, parity, previous abdominal surgery, smoking, comorbidity) were similar between the two groups. There were also no differences in total operation time, length of hospitalization, intraoperative complications, and perioperative change in hemoglobin levels. There was no difference between the two groups in terms of vaginal cuff dehiscence, which was the primary outcome measure of the study. Secondary outcome measures (presence of granulation tissue, spotting, cuff cellulitis) were also similar between the two groups. Conclusions: We observed no differences in outcomes between single-or double-layer vaginal closure techniques with barbed sutures.

Research paper thumbnail of PAPP-A concentrations change in patients with gestational diabetes

Journal of Obstetrics and Gynaecology, 2019

Our aim was to assess the relationship between gestational diabetes and glucose intolerance regar... more Our aim was to assess the relationship between gestational diabetes and glucose intolerance regarding maternal serum PAPP-A and free b-hCG concentrations in first trimester pregnancies. This study was conducted on 278 women between 18-45 years old with singleton pregnancies. The subjects were divided into four groups, according to their 50 and 100 g OGTT results. Group 1 was the Control Group, Group 2 with positive 50 g OGTT results, but negative 100 g, Group 3 had gestational diabetes after testing with 50 g OGTT (180 mg/dl) or with 100 g OGTT. Finally Group 4 was made of women with a one single high glucose level after testing with 100 g OGTT. These groups were analysed in terms of OGTT results. In the GDM group, serum PAPP-A concentrations were significantly lower when compared with the Control Group's (p ¼ 0.015). There was either no significant differences regarding free b-hCG concentrations among the groups. GDM rate is 21.1%, the patients with GDM had significantly low concentrations of serum PAPP-A but their f b-hCG concentrations did not change. Our results are supported by several studies. However, we need greater numbered studies for exact results. IMPACT STATEMENT What is already known on this subject? Pregnancy associated plasma protein A (PAPP-A) is produced by the placenta in pregnancy. PAPP-A cleaves insulin-like growth factor (IGF) binding proteins. It would appear to have a role in regulating IGF bioavailability in pregnancy. This is important as the IGF axis plays a critical role in fetal growth, and placental growth and function during pregnancy. Some studies have reported that PAPP-A levels were impaired among women who subsequently developed GDM. What do the results of this study add? The patients with GDM had significantly low concentrations of serum PAPP-A but their free b-hCG levels did not change. What are the implications of these findings for clinical practice and/or further research? By looking at PAPP-A concentrations, we can predict patients that will be gestational diabetic and take precautions to protect the babies health, such as their diet or exercise.

Research paper thumbnail of Increased pancreatic-derived factor (PANDER) levels in gestational diabetes mellitus

Gynecological Endocrinology, 2019

The aim of the study was to investigate the pancreatic-derived factor (PANDER) levels in healthy ... more The aim of the study was to investigate the pancreatic-derived factor (PANDER) levels in healthy pregnant women and in pregnant women with gestational diabetes mellitus (GDM). A total of 50 women consecutively diagnosed with GDM and 30 randomly selected age-matched and gestational-age-matched healthy pregnant women were included in this cross-sectional study. Serum PANDER levels and other variables were analyzed. The age, the gestational age at the time, the blood sample was obtained and the hemoglobin A1c (HbA1c) levels of the GDM and control groups were similar. The body mass index (BMI), fasting blood glucose, insulin, homeostasis model assessment of insulin resistance (HOMA-IR), and serum PANDER levels were significantly higher in the GDM group than the control group. The optimal PANDER cutoff value was 227.2 ng/ml, and the ratios above this value were 100 and 86.6% for sensitivity and specificity, respectively (p=.0001). Serum PANDER levels were higher in women with GDM compared to the control group and were positively correlated with insulin, HOMA-IR, and HbA1c levels. These results suggest that PANDER might be considered a new biomarker for GDM.

Research paper thumbnail of Wnt signaling pathway in early- and late-onset preeclampsia: evaluation with Dickkopf-1 and R-Spondin-3 glycoproteins

Archives of Gynecology and Obstetrics, 2019

Objective Wnt signaling has been identified as an essential pathway that can direct cell prolifer... more Objective Wnt signaling has been identified as an essential pathway that can direct cell proliferation, migration, and tissue homeostasis. This study aimed to evaluate the role of Wnt signaling pathway in early-onset and late-onset preeclampsia (PE) using serum Dickkopf-1 and R-Spondin-3 glycoproteins. Study design A total of 80 pregnant women were included in this study. The patients were divided into three groups: (1) control (2) early-onset PE, and (3) late-onset PE. The serum levels of Dickkopf-1 and R-Spondin-3 were measured using an enzyme-linked immunosorbent assay. Results Of the 80 pregnant women enrolled in the study, 27 were control, 27 had early-onset PE, and 26 had late-onset PE. No differences were found in the maternal age, gravida, parity, and body mass index among the groups (P = 0.536, 0.230, 0.202, and 0.642, respectively). The serum level of Dickkopf-1 was significantly higher in the early-onset PE group compared with the control group (P = 0.006). The serum level of Dickkopf-1 was statistically similar in control group compared to lateonset PE group (P = 0.064). However, no significant difference was found in the serum levels of Dickkopf-1 and R-Spondin-3 between the early-and late-onset PE groups (P > 0.05). Additionally, the Spearman's correlation analysis revealed a significant negative correlation between maternal serum level of Dickkopf-1 and maternal age (r = − 0.522, P = 0.005). Conclusion The increased serum level of Dickkopf-1 might be associated with the process of pathogenesis of early-onset PE. Further studies would elucidate their exact roles in the pathogenesis of PE.

Research paper thumbnail of dumpyinteracts with a large number of genes in the developing wing ofDrosophila melanogaster

Fly, 2010

Drosophila, dumpy, wing epithelia, apical extracellular matrix, wing blister mutants insert of a ... more Drosophila, dumpy, wing epithelia, apical extracellular matrix, wing blister mutants insert of a repetitive, proline-rich sequence (PR) and by over 30 tandem, nearly identical copies of a novel 102 amino acid repeat that we term the "PIGSFEAST" (PF) repeat since the single letter amino acid code of the region contains these two "words". 13 Near Dumpy's N-terminus are found copies of a sub-class of Ca 2+ binding EGF modules, and a Zona pellucida (ZP) domain is near its C-terminus. The ZP domain is comprised of around 260 amino acids, containing 8 or 10 highly conserved cysteine residues. ZP domains drive polymerization with other ZP domains in either homotypic or heterotypic interactions to form filaments. 14

Research paper thumbnail of PKD is recruited to sites of actin remodelling at the leading edge and negatively regulates cell migration

FEBS Letters, 2007

Protein kinase D (PKD) has been implicated in the regulation of cell shape, adhesion, and migrati... more Protein kinase D (PKD) has been implicated in the regulation of cell shape, adhesion, and migration. At the leading edge of migrating cells active PKD co-localizes with F-actin, Arp3 and cortactin. Platelet derived growth factor (PDGF) activates PKD and recruits the kinase to the leading edge, suggesting a role for PKD in actin remodelling. In support of this, PKD directly interacts with F-actin and phosphorylates cortactin in vitro. Interference with PKD function by overexpression of a dominant negative PKD or by PKD-specific siRNA enhanced cell migration, whereas cells overexpressing PKD wild type displayed reduced migratory potential. Taken together, these data reveal a negative regulatory function of PKD in cell migration.

Research paper thumbnail of Single-layer versus double-layer closure of the vaginal cuff with barbed sutures in laparoscopic hysterectomy

Ginekologia Polska, 2018

Objectives: The utilization of barbed sutures in laparoscopic hysterectomy has become popular amo... more Objectives: The utilization of barbed sutures in laparoscopic hysterectomy has become popular among gynecologic surgeons. Our aim was to compare the outcomes of two different techniques for closing the vaginal cuff with barbed sutures in laparoscopic hysterectomies. Material and methods: A retrospective study was completed on 202 patients who underwent laparoscopic hysterectomy for benign diseases at Istanbul Kanuni Sultan Suleyman Training and Research Hospital from April 2014 through June 2016. In group 1 (n = 139), a single-layer continuous suturing method was used; each bite contained the pubocervical fascia and vaginal mucosa anteriorly, and vaginal mucosa and rectovaginal fascia posteriorly. In group 2 (n = 63), a double-layer continuous suturing method was used; only vaginal mucosa was included in the first layer, and a second layer incorporated the pubocervical and rectovaginal fascias. Results: Patient characteristics (age, body mass index, parity, previous abdominal surgery, smoking, comorbidity) were similar between the two groups. There were also no differences in total operation time, length of hospitalization, intraoperative complications, and perioperative change in hemoglobin levels. There was no difference between the two groups in terms of vaginal cuff dehiscence, which was the primary outcome measure of the study. Secondary outcome measures (presence of granulation tissue, spotting, cuff cellulitis) were also similar between the two groups. Conclusions: We observed no differences in outcomes between single-or double-layer vaginal closure techniques with barbed sutures.

Research paper thumbnail of PAPP-A concentrations change in patients with gestational diabetes

Journal of Obstetrics and Gynaecology, 2019

Our aim was to assess the relationship between gestational diabetes and glucose intolerance regar... more Our aim was to assess the relationship between gestational diabetes and glucose intolerance regarding maternal serum PAPP-A and free b-hCG concentrations in first trimester pregnancies. This study was conducted on 278 women between 18-45 years old with singleton pregnancies. The subjects were divided into four groups, according to their 50 and 100 g OGTT results. Group 1 was the Control Group, Group 2 with positive 50 g OGTT results, but negative 100 g, Group 3 had gestational diabetes after testing with 50 g OGTT (180 mg/dl) or with 100 g OGTT. Finally Group 4 was made of women with a one single high glucose level after testing with 100 g OGTT. These groups were analysed in terms of OGTT results. In the GDM group, serum PAPP-A concentrations were significantly lower when compared with the Control Group's (p ¼ 0.015). There was either no significant differences regarding free b-hCG concentrations among the groups. GDM rate is 21.1%, the patients with GDM had significantly low concentrations of serum PAPP-A but their f b-hCG concentrations did not change. Our results are supported by several studies. However, we need greater numbered studies for exact results. IMPACT STATEMENT What is already known on this subject? Pregnancy associated plasma protein A (PAPP-A) is produced by the placenta in pregnancy. PAPP-A cleaves insulin-like growth factor (IGF) binding proteins. It would appear to have a role in regulating IGF bioavailability in pregnancy. This is important as the IGF axis plays a critical role in fetal growth, and placental growth and function during pregnancy. Some studies have reported that PAPP-A levels were impaired among women who subsequently developed GDM. What do the results of this study add? The patients with GDM had significantly low concentrations of serum PAPP-A but their free b-hCG levels did not change. What are the implications of these findings for clinical practice and/or further research? By looking at PAPP-A concentrations, we can predict patients that will be gestational diabetic and take precautions to protect the babies health, such as their diet or exercise.

Research paper thumbnail of Increased pancreatic-derived factor (PANDER) levels in gestational diabetes mellitus

Gynecological Endocrinology, 2019

The aim of the study was to investigate the pancreatic-derived factor (PANDER) levels in healthy ... more The aim of the study was to investigate the pancreatic-derived factor (PANDER) levels in healthy pregnant women and in pregnant women with gestational diabetes mellitus (GDM). A total of 50 women consecutively diagnosed with GDM and 30 randomly selected age-matched and gestational-age-matched healthy pregnant women were included in this cross-sectional study. Serum PANDER levels and other variables were analyzed. The age, the gestational age at the time, the blood sample was obtained and the hemoglobin A1c (HbA1c) levels of the GDM and control groups were similar. The body mass index (BMI), fasting blood glucose, insulin, homeostasis model assessment of insulin resistance (HOMA-IR), and serum PANDER levels were significantly higher in the GDM group than the control group. The optimal PANDER cutoff value was 227.2 ng/ml, and the ratios above this value were 100 and 86.6% for sensitivity and specificity, respectively (p=.0001). Serum PANDER levels were higher in women with GDM compared to the control group and were positively correlated with insulin, HOMA-IR, and HbA1c levels. These results suggest that PANDER might be considered a new biomarker for GDM.

Research paper thumbnail of Wnt signaling pathway in early- and late-onset preeclampsia: evaluation with Dickkopf-1 and R-Spondin-3 glycoproteins

Archives of Gynecology and Obstetrics, 2019

Objective Wnt signaling has been identified as an essential pathway that can direct cell prolifer... more Objective Wnt signaling has been identified as an essential pathway that can direct cell proliferation, migration, and tissue homeostasis. This study aimed to evaluate the role of Wnt signaling pathway in early-onset and late-onset preeclampsia (PE) using serum Dickkopf-1 and R-Spondin-3 glycoproteins. Study design A total of 80 pregnant women were included in this study. The patients were divided into three groups: (1) control (2) early-onset PE, and (3) late-onset PE. The serum levels of Dickkopf-1 and R-Spondin-3 were measured using an enzyme-linked immunosorbent assay. Results Of the 80 pregnant women enrolled in the study, 27 were control, 27 had early-onset PE, and 26 had late-onset PE. No differences were found in the maternal age, gravida, parity, and body mass index among the groups (P = 0.536, 0.230, 0.202, and 0.642, respectively). The serum level of Dickkopf-1 was significantly higher in the early-onset PE group compared with the control group (P = 0.006). The serum level of Dickkopf-1 was statistically similar in control group compared to lateonset PE group (P = 0.064). However, no significant difference was found in the serum levels of Dickkopf-1 and R-Spondin-3 between the early-and late-onset PE groups (P > 0.05). Additionally, the Spearman's correlation analysis revealed a significant negative correlation between maternal serum level of Dickkopf-1 and maternal age (r = − 0.522, P = 0.005). Conclusion The increased serum level of Dickkopf-1 might be associated with the process of pathogenesis of early-onset PE. Further studies would elucidate their exact roles in the pathogenesis of PE.

Research paper thumbnail of dumpyinteracts with a large number of genes in the developing wing ofDrosophila melanogaster

Fly, 2010

Drosophila, dumpy, wing epithelia, apical extracellular matrix, wing blister mutants insert of a ... more Drosophila, dumpy, wing epithelia, apical extracellular matrix, wing blister mutants insert of a repetitive, proline-rich sequence (PR) and by over 30 tandem, nearly identical copies of a novel 102 amino acid repeat that we term the "PIGSFEAST" (PF) repeat since the single letter amino acid code of the region contains these two "words". 13 Near Dumpy's N-terminus are found copies of a sub-class of Ca 2+ binding EGF modules, and a Zona pellucida (ZP) domain is near its C-terminus. The ZP domain is comprised of around 260 amino acids, containing 8 or 10 highly conserved cysteine residues. ZP domains drive polymerization with other ZP domains in either homotypic or heterotypic interactions to form filaments. 14

Research paper thumbnail of PKD is recruited to sites of actin remodelling at the leading edge and negatively regulates cell migration

FEBS Letters, 2007

Protein kinase D (PKD) has been implicated in the regulation of cell shape, adhesion, and migrati... more Protein kinase D (PKD) has been implicated in the regulation of cell shape, adhesion, and migration. At the leading edge of migrating cells active PKD co-localizes with F-actin, Arp3 and cortactin. Platelet derived growth factor (PDGF) activates PKD and recruits the kinase to the leading edge, suggesting a role for PKD in actin remodelling. In support of this, PKD directly interacts with F-actin and phosphorylates cortactin in vitro. Interference with PKD function by overexpression of a dominant negative PKD or by PKD-specific siRNA enhanced cell migration, whereas cells overexpressing PKD wild type displayed reduced migratory potential. Taken together, these data reveal a negative regulatory function of PKD in cell migration.