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Papers by Flavia Garcia

Ce document est protégé par la loi sur le droit d'auteur. L'utilisation des services d'Érudit (y ... more Ce document est protégé par la loi sur le droit d'auteur. L'utilisation des services d'Érudit (y compris la reproduction) est assujettie à sa politique d'utilisation que vous pouvez consulter en ligne.

Molecular and Cellular Biochemistry, 2021

Intravascular hemolysis, a major manifestation of sickle cell disease (SCD) and other diseases, i... more Intravascular hemolysis, a major manifestation of sickle cell disease (SCD) and other diseases, incurs the release of hemoglobin and heme from red blood cells, in turn triggering inflammatory processes. This study investigated the in vitro effects of heme, a major inflammatory DAMP, on the adhesive properties of isolated human neutrophils. Heme (20 and 50 µM) significantly increased the adhesion of neutrophils to fibronectin and to recombinant ICAM-1, under static conditions, even more efficiently than the potent pro-inflammatory cytokine, tumor necrosis factor-α (TNF); a microfluidic assay confirmed that heme stimulated neutrophil adhesion under conditions of shear stress. Heme-induced neutrophil adhesion was associated with the increased activities, but not expressions, of the Mac-1 and LFA-1 integrin subunits, CD11b and CD11a, on the cell surface. Notably, heme (50 µM) significantly induced NFκB translocation in neutrophils, and inhibition of NFκB activity with the BAY11-7082 molecule abolished heme-induced cell adhesion to fibronectin and significantly decreased CD11a activity. Flow cytometric analysis demonstrated major reactive oxygen species (ROS) generation in neutrophils following heme stimulation that could be inhibited by the antioxidant, α-tocopherol, and by BAY11-7082. Furthermore, co-incubation with α-tocopherol abrogated both heme-stimulated neutrophil adhesion and CD11a/CD11b activation. Thus, our data indicate that heme, at clinically relevant concentrations, is a potent activator of neutrophil adhesion, increasing the ligand affinity of the β2 integrins via a mechanism that may be partially mediated by an NFkB-dependent pathway and the generation of ROS. Given the fundamental role that the adhesion of neutrophils to the vascular wall plays in SCD vaso-occlusion and other vascular inflammatory processes, our findings provide further evidence that cell-free heme is a major therapeutic target in the hemolytic diseases.

Orientador: Leonilda Maria Barbosa dos SantosDissertação (mestrado) - Universidade Estadual de Ca... more Orientador: Leonilda Maria Barbosa dos SantosDissertação (mestrado) - Universidade Estadual de Campinas, Instituto de BiologiaMestrad

Scientific Reports, 2020

Leukocyte recruitment and heterocellular aggregate formation drive the inflammatory vaso-occlusiv... more Leukocyte recruitment and heterocellular aggregate formation drive the inflammatory vaso-occlusive processes associated with sickle cell anemia (SCA). We characterized neutrophils in a population of patients with SCA and investigated whether platelet-derived molecules can induce phenotypic alterations in this cell type. Imaging flow cytometry analysis demonstrated that the frequency of circulating CXCR4hi neutrophils was significantly higher in steady-state SCA individuals than in healthy control individuals and that these cells presented increased CD11b activation and toll-like receptor-4 expression. SCA neutrophils display increased neutrophil-platelet aggregation, and CXCR4hi neutrophils demonstrated augmented neutrophil-platelet aggregate frequency with a higher mean number of platelets adhered per neutrophil. Importantly, incubation of neutrophils with platelets significantly elevated their CXCR4 expression, while SCA plasma was found to induce CXCR4hi neutrophil polarization s...

Journal of Pharmacology and Experimental Therapeutics, 2020

Soluble guanylyl cyclase stimulation in sickle cell disease.

Blood, 2018

Background: Vaso-occlusive processes in sickle cell anemia (SCA) are triggered by inflammatory mo... more Background: Vaso-occlusive processes in sickle cell anemia (SCA) are triggered by inflammatory molecules, along with pancellular activation and the recruitment and adhesion of leukocytes to the endothelium. Interactions between platelets, neutrophils, and erythrocytes are observed in the SCA circulation, but the exact role of platelets in the initiation and propagation of vaso-occlusion is not well understood. Aim: We, herein, evaluated the effects of the inhibition of platelet activation on TNF-α (TNF)-triggered vaso-occlusive processes in the microcirculation of mice with SCA, using prasugrel, a platelet ADP P2Y12 receptor inhibitor. Methods: Chimeric male control (CON) and SCA (SCA) mice received prasugrel (1 mg/kg, by gavage) or vehicle (saline) in a single administration, 1h prior to TNF (0.5 μg/g, i.p). In some experiments, 1 mg/kg prasugrel was administered chronically for 5x per week for 4 weeks. Following i.v. injection of anti-CD41 PE, anti-CD45 PeCy-5 and anti-Ly6G Alexa ...

Xyz La Revue De La Nouvelle, 2014

MOJ Immunology, 2016

The effective therapy for illnesses associated to the excess, or lack, of immunological response ... more The effective therapy for illnesses associated to the excess, or lack, of immunological response depends on the advances in knowledge about the effects of neurotransmitters on the leukocytes activity. The HPA axis and the autonomous nervous system control inflammation, while certain substances secreted locally by peripheral nervous system tend to enhance the inflammatory response. In this last case, possibly as a support mechanism to the elimination of an infectious agent. Data available on the literature are still scarce, but they already emphasize the Neuroimmunology as a promising field of investigation and, above all, necessary.

Ao meu orientador Prof. Dr. Luiz Henrique Catalani, pela amizade, oportunidade de realizar este t... more Ao meu orientador Prof. Dr. Luiz Henrique Catalani, pela amizade, oportunidade de realizar este trabalho, permitindo que eu voasse sozinha, mas me socorrendo quando necessário. Também pela severidade na orientação, que muito me enriqueceu intelectualmente. Minha admiração especial. A Profa. Ora. Ana Campa, meu porto seguro na pós-graduação, pela colaboração científica, confiança, amizade e por me fazer batalhar, aprender, crescer. Minha eterna gratidão. Ao Prof. Dr. Paolo Di Mascio pelo apoio científico, valiosa colaboração para a realização de parte importante deste trabalho, paciência e amizade. A Gláucia minha amiga, pelos ensinamentos científicos, paciência, incentivo e carinho. Minha gratidão especial. A especialista Fernanda, pelos ensinamentos científicos do espectro de massa, paciência e amizade. A Profa. Ora. Célia Regina Garcia pela disponibilizarão do Microscópio eletrônico confocal e a seu aluno Flávio, pela ajuda técnica científica das imagens de microscopia. Ao Prof. Dr. Momtchilo Russo do ICB e a Eliana (Lili) sua aluna, pelo apoio científico. A Profa. Maria Teresa, minha mãe, pela ajuda nas correções gramaticais deste trabalho. A todos os amigos do laboratório: Vânia, Guilhermino, Luiz Carlos, Silvia, Reginaldo, Patrícia, Romeu, Aline e Claudete, pelo sangue tão precioso, pelo companheirismo, paciência e incentivo e em especial, a Janaína pela amizade, dedicação e carinho em todos os momentos e ao Antônio, pelo apoio e amizade. Considero a todos como uma dádiva divina. Ao Valdecir grande amigo, pelas sugestões e pelo valioso carinho. Ao grupo do laboratório da Ana: Elaine, Cristiane, Silvana, Sabrina, Flávia Mammy, Silene, Rose e Alziana pela convivência e apoio, em especial a Maria Rita, pelo incentivo e amizade, e a Sueli, pela contribuição científica, carinho e apoio. A todos os colegas de convívio e amizade no

Revista Brasileira de Botânica, 2005

Twenty species of 13 genera of Papilionoideae were identified. The genera that had more represent... more Twenty species of 13 genera of Papilionoideae were identified. The genera that had more representative number of species were Desmodium (4), Crotalaria (3), Machaerium (2) and Stylosanthes (2). The others had only one specie each. In the phenological study of the species, correlations between environmental parameters (temperature and rainfall) and the leaf fall, leaf flushing, flowering and the fruiting were verified. Key words-"campos ferruginosos", floristic, Itacolomi State Park, Leguminosae-Papilionoideae, phenology RESUMO-(Papilionoideae (Leguminosae) dos campos ferruginosos do Parque Estadual do Itacolomi, MG). Foi realizado um levantamento florístico das espécies de Papilionoideae (Leguminosae) ocorrentes nos campos ferruginosos do Parque Estadual do Itacolomi (PEI), situado nos municípios de Ouro Preto e Mariana, estado de Minas Gerais. As coletas foram feitas no período de março de 2001 a maio de 2002. Foram identificadas 20 espécies de Papilionoideae, pertencentes a 13 gêneros. Os gêneros mais representativos em número de espécies foram Desmodium (4), Crotalaria (3), Machaerium e Stylosanthes, com duas espécies cada. Os demais gêneros foram representados por apenas uma espécie cada. No estudo fenológico das espécies, foram constatadas correlações entre fatores climáticos (temperatura e precipitação) e a perda de folhas, brotação, floração e frutificação.

Neurobiology of Aging, 2004

The Role of Caregiver, Formal and~or Informal patients' behaviour, this may suggest that carets w... more The Role of Caregiver, Formal and~or Informal patients' behaviour, this may suggest that carets who internalise may benefit from a behaviour management training intervention. Family conflict is also shown to correlate with depression which highlights other target areas for future intervention programmes.

Neotropical Entomology, 2007

Biology of Acarophenax lacunatus (Cross & Krantz) (Prostigmata: Acarophenacidae) on Tribolium cas... more Biology of Acarophenax lacunatus (Cross & Krantz) (Prostigmata: Acarophenacidae) on Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) and Cryptolestes ferrugineus (Stephens) (Coleoptera: Cucujidae) ABSTRACT-The aim of the present study was to assess the effect of six different temperatures on the development of Acarophenax lacunatus (Cross & Krantz) using eggs of Tribolium castaneum (Herbst) and Cryptolestes ferrugineus (Stephens) as hosts. The temperature affected the development of A. lacunatus. The largest values for the progeny (19 mites in T. castaneum and 15 mites in C. ferrugineus) were obtained at about 30 o C, as also observed for the net reproductive rate (R o), which revealed that the A. lacunatus population increased 18 times in T. castaneum and 14 times in C. ferrugineus in a generation. The intrinsic rate of increase (r m) gradually increased with temperature, reaching the maximum value at 35 o C in T. castaneum (1,608) and C. ferrugineus (1,289). The generation time was negatively correlated with temperature, ranging from 1,60 to 4,85 days in T. castaneum and from 1,96 to 5,34 days in C. ferrugineus. These results suggest that the mite A. lacunatus may be used in programs of biological control of T. castaneum and C. ferrugineus in the tropics.

Neotropical Entomology, 2006

Interaction between Acarophenax lacunatus (Cross & Krantz) (Prostigmata: Acarophenacidae) and Ani... more Interaction between Acarophenax lacunatus (Cross & Krantz) (Prostigmata: Acarophenacidae) and Anisopteromalus calandrae (Howard) (Hymenoptera: Pteromalidae) on Rhyzopertha dominica (Fabricius) (Coleoptera: Bostrichidae) ABSTRACT-The interaction between Acarophenax lacunatus (Cross & Krantz) and Anisopteromalus calandrae (Howard) may be a promising tool for the integrated pest management of stored grain insect pests. The objective of this study was to evaluate the compatibility of these two natural enemies on Rhyzopertha dominica (Fabricius). The experimental units were petri dishes (140 x 10 mm) containing 30 g of whole wheat grains (13% water content) infested with 20 adults of R. dominica. The treatments consisted of inoculation of A. lacunatus and A. calandrae, separately and associated, in eight replicates. Three inoculations of fi ve adult females of the natural enemies were carried out in each petri dish at fi ve, ten and fi fteen days after the infestation of R. dominica. All treatments were stored during 60 days in environmental chamber at 30 ± 1°C, 60 ± 5% relative humidity and 24h scotophase. The smallest numbers of physogastric females of A. lacunatus and of adults of A. calandrae were obtained when the natural enemies were in association. The use of A. calandrae alone demonstrated a low instantaneous rate of increase (r i) of R. dominica and a high protection of the wheat grains. The association of A. calandrae with A. lacunatus led to the lowest number of immatures of R. dominica. These results demonstrate the importance of this interaction as a tool of for the integrated management of R. dominica in stored wheat grains.

Journal of Leukocyte Biology, 2011

Intrathymic T cell differentiation takes place within the thymic lobules and depends on interacti... more Intrathymic T cell differentiation takes place within the thymic lobules and depends on interactions between developing thymocytes and cells of the thymic microenvironment. Along with differentiation, thymocytes migrate in an oriented progression, which is tightly regulated by a number of interactions, including one mediated by the chemokine CXCL12. It has been shown recently that SEMA-3A, a soluble member of the semaphorin family, is also involved in this human thymocyte migration and can have a chemorepulsive and de-adhesive role. Herein, we study the role of SEMA-3A on the CXCL12-driven migration of human thymocytes. We have shown that SEMA-3A is able to inhibit the chemotaxis triggered by CXCL12. Such an inhibition was seen in respect to immature and mature CD4/CD8-defined thymocyte subsets and can be reverted specifically by neutralizing anti-SEMA-3A mAb. We have also shown that SEMA-3A consistently down-regulates CXCR4 membrane expression in all CD4/CD8-defined thymocyte subse...

Alzheimer's & Dementia, 2006

Acta Botanica Brasilica, 2006

Este trabalho consiste no levantamento florístico e estudo taxonômico da família Rubiaceae, da Re... more Este trabalho consiste no levantamento florístico e estudo taxonômico da família Rubiaceae, da Reserva Florestal Mata do Paraíso, Viçosa, MG. Foram registrados para a Reserva 14 gêneros compreendendo 30 espécies. Destas, 19 apresentaram hábito arbustivo e 11 são herbáceos. Os gêneros com maior número de espécies foram Psychotria (10 espécies), Borreria (quatro), Coccocypselum (três) e Coussarea e Palicourea (duas). Bathysa, Chiococca, Coutarea, Diodia, Faramea, Galium, Mitracarpus, Richardia e Rudgea apresentaram uma única espécie cada. São fornecidas neste trabalho chaves analíticas, descrições, ilustrações, comentários taxonômicos e distribuição geográfica para cada espécie.

Acta Botanica Brasilica, 2006

O presente trabalho consta do estudo taxonômico das espécies de Araceae que ocorrem no Parque Est... more O presente trabalho consta do estudo taxonômico das espécies de Araceae que ocorrem no Parque Estadual do Rio Doce, com chaves dicotômicas, descrições e ilustrações botânicas. Foram realizadas excursões mensais de maio/1999 a abril/2000 para coleta do material botânico, ao longo de 11 trilhas preestabelecidas nas regiões central e sul do parque. São reconhecidas 13 espécies, pertencentes a oito gêneros, sendo Philodendron o mais representativo com três espécies. Quatro das espécies encontradas no Parque são novos registros para Minas Gerais: Asterostigma concinnum Schott, Heteropsis flexuosa (Kunth) G.S. Bunting, Philodendron speciosum Schott e Philodendron vargealtense Sakuragui.

Ce document est protégé par la loi sur le droit d'auteur. L'utilisation des services d'Érudit (y ... more Ce document est protégé par la loi sur le droit d'auteur. L'utilisation des services d'Érudit (y compris la reproduction) est assujettie à sa politique d'utilisation que vous pouvez consulter en ligne.

Molecular and Cellular Biochemistry, 2021

Intravascular hemolysis, a major manifestation of sickle cell disease (SCD) and other diseases, i... more Intravascular hemolysis, a major manifestation of sickle cell disease (SCD) and other diseases, incurs the release of hemoglobin and heme from red blood cells, in turn triggering inflammatory processes. This study investigated the in vitro effects of heme, a major inflammatory DAMP, on the adhesive properties of isolated human neutrophils. Heme (20 and 50 µM) significantly increased the adhesion of neutrophils to fibronectin and to recombinant ICAM-1, under static conditions, even more efficiently than the potent pro-inflammatory cytokine, tumor necrosis factor-α (TNF); a microfluidic assay confirmed that heme stimulated neutrophil adhesion under conditions of shear stress. Heme-induced neutrophil adhesion was associated with the increased activities, but not expressions, of the Mac-1 and LFA-1 integrin subunits, CD11b and CD11a, on the cell surface. Notably, heme (50 µM) significantly induced NFκB translocation in neutrophils, and inhibition of NFκB activity with the BAY11-7082 molecule abolished heme-induced cell adhesion to fibronectin and significantly decreased CD11a activity. Flow cytometric analysis demonstrated major reactive oxygen species (ROS) generation in neutrophils following heme stimulation that could be inhibited by the antioxidant, α-tocopherol, and by BAY11-7082. Furthermore, co-incubation with α-tocopherol abrogated both heme-stimulated neutrophil adhesion and CD11a/CD11b activation. Thus, our data indicate that heme, at clinically relevant concentrations, is a potent activator of neutrophil adhesion, increasing the ligand affinity of the β2 integrins via a mechanism that may be partially mediated by an NFkB-dependent pathway and the generation of ROS. Given the fundamental role that the adhesion of neutrophils to the vascular wall plays in SCD vaso-occlusion and other vascular inflammatory processes, our findings provide further evidence that cell-free heme is a major therapeutic target in the hemolytic diseases.

Orientador: Leonilda Maria Barbosa dos SantosDissertação (mestrado) - Universidade Estadual de Ca... more Orientador: Leonilda Maria Barbosa dos SantosDissertação (mestrado) - Universidade Estadual de Campinas, Instituto de BiologiaMestrad

Scientific Reports, 2020

Leukocyte recruitment and heterocellular aggregate formation drive the inflammatory vaso-occlusiv... more Leukocyte recruitment and heterocellular aggregate formation drive the inflammatory vaso-occlusive processes associated with sickle cell anemia (SCA). We characterized neutrophils in a population of patients with SCA and investigated whether platelet-derived molecules can induce phenotypic alterations in this cell type. Imaging flow cytometry analysis demonstrated that the frequency of circulating CXCR4hi neutrophils was significantly higher in steady-state SCA individuals than in healthy control individuals and that these cells presented increased CD11b activation and toll-like receptor-4 expression. SCA neutrophils display increased neutrophil-platelet aggregation, and CXCR4hi neutrophils demonstrated augmented neutrophil-platelet aggregate frequency with a higher mean number of platelets adhered per neutrophil. Importantly, incubation of neutrophils with platelets significantly elevated their CXCR4 expression, while SCA plasma was found to induce CXCR4hi neutrophil polarization s...

Journal of Pharmacology and Experimental Therapeutics, 2020

Soluble guanylyl cyclase stimulation in sickle cell disease.

Blood, 2018

Background: Vaso-occlusive processes in sickle cell anemia (SCA) are triggered by inflammatory mo... more Background: Vaso-occlusive processes in sickle cell anemia (SCA) are triggered by inflammatory molecules, along with pancellular activation and the recruitment and adhesion of leukocytes to the endothelium. Interactions between platelets, neutrophils, and erythrocytes are observed in the SCA circulation, but the exact role of platelets in the initiation and propagation of vaso-occlusion is not well understood. Aim: We, herein, evaluated the effects of the inhibition of platelet activation on TNF-α (TNF)-triggered vaso-occlusive processes in the microcirculation of mice with SCA, using prasugrel, a platelet ADP P2Y12 receptor inhibitor. Methods: Chimeric male control (CON) and SCA (SCA) mice received prasugrel (1 mg/kg, by gavage) or vehicle (saline) in a single administration, 1h prior to TNF (0.5 μg/g, i.p). In some experiments, 1 mg/kg prasugrel was administered chronically for 5x per week for 4 weeks. Following i.v. injection of anti-CD41 PE, anti-CD45 PeCy-5 and anti-Ly6G Alexa ...

Xyz La Revue De La Nouvelle, 2014

MOJ Immunology, 2016

The effective therapy for illnesses associated to the excess, or lack, of immunological response ... more The effective therapy for illnesses associated to the excess, or lack, of immunological response depends on the advances in knowledge about the effects of neurotransmitters on the leukocytes activity. The HPA axis and the autonomous nervous system control inflammation, while certain substances secreted locally by peripheral nervous system tend to enhance the inflammatory response. In this last case, possibly as a support mechanism to the elimination of an infectious agent. Data available on the literature are still scarce, but they already emphasize the Neuroimmunology as a promising field of investigation and, above all, necessary.

Ao meu orientador Prof. Dr. Luiz Henrique Catalani, pela amizade, oportunidade de realizar este t... more Ao meu orientador Prof. Dr. Luiz Henrique Catalani, pela amizade, oportunidade de realizar este trabalho, permitindo que eu voasse sozinha, mas me socorrendo quando necessário. Também pela severidade na orientação, que muito me enriqueceu intelectualmente. Minha admiração especial. A Profa. Ora. Ana Campa, meu porto seguro na pós-graduação, pela colaboração científica, confiança, amizade e por me fazer batalhar, aprender, crescer. Minha eterna gratidão. Ao Prof. Dr. Paolo Di Mascio pelo apoio científico, valiosa colaboração para a realização de parte importante deste trabalho, paciência e amizade. A Gláucia minha amiga, pelos ensinamentos científicos, paciência, incentivo e carinho. Minha gratidão especial. A especialista Fernanda, pelos ensinamentos científicos do espectro de massa, paciência e amizade. A Profa. Ora. Célia Regina Garcia pela disponibilizarão do Microscópio eletrônico confocal e a seu aluno Flávio, pela ajuda técnica científica das imagens de microscopia. Ao Prof. Dr. Momtchilo Russo do ICB e a Eliana (Lili) sua aluna, pelo apoio científico. A Profa. Maria Teresa, minha mãe, pela ajuda nas correções gramaticais deste trabalho. A todos os amigos do laboratório: Vânia, Guilhermino, Luiz Carlos, Silvia, Reginaldo, Patrícia, Romeu, Aline e Claudete, pelo sangue tão precioso, pelo companheirismo, paciência e incentivo e em especial, a Janaína pela amizade, dedicação e carinho em todos os momentos e ao Antônio, pelo apoio e amizade. Considero a todos como uma dádiva divina. Ao Valdecir grande amigo, pelas sugestões e pelo valioso carinho. Ao grupo do laboratório da Ana: Elaine, Cristiane, Silvana, Sabrina, Flávia Mammy, Silene, Rose e Alziana pela convivência e apoio, em especial a Maria Rita, pelo incentivo e amizade, e a Sueli, pela contribuição científica, carinho e apoio. A todos os colegas de convívio e amizade no

Revista Brasileira de Botânica, 2005

Twenty species of 13 genera of Papilionoideae were identified. The genera that had more represent... more Twenty species of 13 genera of Papilionoideae were identified. The genera that had more representative number of species were Desmodium (4), Crotalaria (3), Machaerium (2) and Stylosanthes (2). The others had only one specie each. In the phenological study of the species, correlations between environmental parameters (temperature and rainfall) and the leaf fall, leaf flushing, flowering and the fruiting were verified. Key words-"campos ferruginosos", floristic, Itacolomi State Park, Leguminosae-Papilionoideae, phenology RESUMO-(Papilionoideae (Leguminosae) dos campos ferruginosos do Parque Estadual do Itacolomi, MG). Foi realizado um levantamento florístico das espécies de Papilionoideae (Leguminosae) ocorrentes nos campos ferruginosos do Parque Estadual do Itacolomi (PEI), situado nos municípios de Ouro Preto e Mariana, estado de Minas Gerais. As coletas foram feitas no período de março de 2001 a maio de 2002. Foram identificadas 20 espécies de Papilionoideae, pertencentes a 13 gêneros. Os gêneros mais representativos em número de espécies foram Desmodium (4), Crotalaria (3), Machaerium e Stylosanthes, com duas espécies cada. Os demais gêneros foram representados por apenas uma espécie cada. No estudo fenológico das espécies, foram constatadas correlações entre fatores climáticos (temperatura e precipitação) e a perda de folhas, brotação, floração e frutificação.

Neurobiology of Aging, 2004

The Role of Caregiver, Formal and~or Informal patients' behaviour, this may suggest that carets w... more The Role of Caregiver, Formal and~or Informal patients' behaviour, this may suggest that carets who internalise may benefit from a behaviour management training intervention. Family conflict is also shown to correlate with depression which highlights other target areas for future intervention programmes.

Neotropical Entomology, 2007

Biology of Acarophenax lacunatus (Cross & Krantz) (Prostigmata: Acarophenacidae) on Tribolium cas... more Biology of Acarophenax lacunatus (Cross & Krantz) (Prostigmata: Acarophenacidae) on Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) and Cryptolestes ferrugineus (Stephens) (Coleoptera: Cucujidae) ABSTRACT-The aim of the present study was to assess the effect of six different temperatures on the development of Acarophenax lacunatus (Cross & Krantz) using eggs of Tribolium castaneum (Herbst) and Cryptolestes ferrugineus (Stephens) as hosts. The temperature affected the development of A. lacunatus. The largest values for the progeny (19 mites in T. castaneum and 15 mites in C. ferrugineus) were obtained at about 30 o C, as also observed for the net reproductive rate (R o), which revealed that the A. lacunatus population increased 18 times in T. castaneum and 14 times in C. ferrugineus in a generation. The intrinsic rate of increase (r m) gradually increased with temperature, reaching the maximum value at 35 o C in T. castaneum (1,608) and C. ferrugineus (1,289). The generation time was negatively correlated with temperature, ranging from 1,60 to 4,85 days in T. castaneum and from 1,96 to 5,34 days in C. ferrugineus. These results suggest that the mite A. lacunatus may be used in programs of biological control of T. castaneum and C. ferrugineus in the tropics.

Neotropical Entomology, 2006

Interaction between Acarophenax lacunatus (Cross & Krantz) (Prostigmata: Acarophenacidae) and Ani... more Interaction between Acarophenax lacunatus (Cross & Krantz) (Prostigmata: Acarophenacidae) and Anisopteromalus calandrae (Howard) (Hymenoptera: Pteromalidae) on Rhyzopertha dominica (Fabricius) (Coleoptera: Bostrichidae) ABSTRACT-The interaction between Acarophenax lacunatus (Cross & Krantz) and Anisopteromalus calandrae (Howard) may be a promising tool for the integrated pest management of stored grain insect pests. The objective of this study was to evaluate the compatibility of these two natural enemies on Rhyzopertha dominica (Fabricius). The experimental units were petri dishes (140 x 10 mm) containing 30 g of whole wheat grains (13% water content) infested with 20 adults of R. dominica. The treatments consisted of inoculation of A. lacunatus and A. calandrae, separately and associated, in eight replicates. Three inoculations of fi ve adult females of the natural enemies were carried out in each petri dish at fi ve, ten and fi fteen days after the infestation of R. dominica. All treatments were stored during 60 days in environmental chamber at 30 ± 1°C, 60 ± 5% relative humidity and 24h scotophase. The smallest numbers of physogastric females of A. lacunatus and of adults of A. calandrae were obtained when the natural enemies were in association. The use of A. calandrae alone demonstrated a low instantaneous rate of increase (r i) of R. dominica and a high protection of the wheat grains. The association of A. calandrae with A. lacunatus led to the lowest number of immatures of R. dominica. These results demonstrate the importance of this interaction as a tool of for the integrated management of R. dominica in stored wheat grains.

Journal of Leukocyte Biology, 2011

Intrathymic T cell differentiation takes place within the thymic lobules and depends on interacti... more Intrathymic T cell differentiation takes place within the thymic lobules and depends on interactions between developing thymocytes and cells of the thymic microenvironment. Along with differentiation, thymocytes migrate in an oriented progression, which is tightly regulated by a number of interactions, including one mediated by the chemokine CXCL12. It has been shown recently that SEMA-3A, a soluble member of the semaphorin family, is also involved in this human thymocyte migration and can have a chemorepulsive and de-adhesive role. Herein, we study the role of SEMA-3A on the CXCL12-driven migration of human thymocytes. We have shown that SEMA-3A is able to inhibit the chemotaxis triggered by CXCL12. Such an inhibition was seen in respect to immature and mature CD4/CD8-defined thymocyte subsets and can be reverted specifically by neutralizing anti-SEMA-3A mAb. We have also shown that SEMA-3A consistently down-regulates CXCR4 membrane expression in all CD4/CD8-defined thymocyte subse...

Alzheimer's & Dementia, 2006

Acta Botanica Brasilica, 2006

Este trabalho consiste no levantamento florístico e estudo taxonômico da família Rubiaceae, da Re... more Este trabalho consiste no levantamento florístico e estudo taxonômico da família Rubiaceae, da Reserva Florestal Mata do Paraíso, Viçosa, MG. Foram registrados para a Reserva 14 gêneros compreendendo 30 espécies. Destas, 19 apresentaram hábito arbustivo e 11 são herbáceos. Os gêneros com maior número de espécies foram Psychotria (10 espécies), Borreria (quatro), Coccocypselum (três) e Coussarea e Palicourea (duas). Bathysa, Chiococca, Coutarea, Diodia, Faramea, Galium, Mitracarpus, Richardia e Rudgea apresentaram uma única espécie cada. São fornecidas neste trabalho chaves analíticas, descrições, ilustrações, comentários taxonômicos e distribuição geográfica para cada espécie.

Acta Botanica Brasilica, 2006

O presente trabalho consta do estudo taxonômico das espécies de Araceae que ocorrem no Parque Est... more O presente trabalho consta do estudo taxonômico das espécies de Araceae que ocorrem no Parque Estadual do Rio Doce, com chaves dicotômicas, descrições e ilustrações botânicas. Foram realizadas excursões mensais de maio/1999 a abril/2000 para coleta do material botânico, ao longo de 11 trilhas preestabelecidas nas regiões central e sul do parque. São reconhecidas 13 espécies, pertencentes a oito gêneros, sendo Philodendron o mais representativo com três espécies. Quatro das espécies encontradas no Parque são novos registros para Minas Gerais: Asterostigma concinnum Schott, Heteropsis flexuosa (Kunth) G.S. Bunting, Philodendron speciosum Schott e Philodendron vargealtense Sakuragui.