Glenn Foulds - Academia.edu (original) (raw)

Papers by Glenn Foulds

Research paper thumbnail of Photoaptamer arrays applied to multiplexed proteomic analysis

Proteomics, Mar 1, 2004

Multiplexed photoaptamer‐based arrays that allow for the simultaneous measurement of multiple pro... more Multiplexed photoaptamer‐based arrays that allow for the simultaneous measurement of multiple proteins of interest in serum samples are described. Since photoaptamers covalently bind to their target analytes before fluorescent signal detection, the arrays can be vigorously washed to remove background proteins, providing the potential for superior signal‐to‐noise ratios and lower limits of quantification in biological matrices. Data are presented here for a 17‐plex photoaptamer array exhibiting limits of detection below 10 fM for several analytes including interleukin‐16, vascular endothelial growth factor, and endostatin and able to measure proteins in 10% serum samples. The assays are simple, scalable, and reproducible. Affinity of the capture reagent is shown to be directly correlated to the limit of detection for the analyte on the array.

Research paper thumbnail of Protein-DNA Binding Affinities by Capillary Electrophoresis

Humana Press eBooks, Nov 15, 2003

ABSTRACT Described in this chapter is a capillary electrophoresis (CE) method for the accurate de... more ABSTRACT Described in this chapter is a capillary electrophoresis (CE) method for the accurate determination of protein-DNA binding constants (1). The advantages of the method include the fast separation and quantitation of protein-DNA complex and free DNA, in uncoated capillaries without sieving matrices, coupled with LIF detection of fluorescently-labeled DNA at nM to πM concentrations.

Research paper thumbnail of Chemistry of the mycalamides: antiviral and antitumour compounds from a New Zealand marine sponge. Part 6.1–3 The synthesis and testing of analogues of the C(7)–C(10) fragment

Journal of the Chemical Society, 1997

Research paper thumbnail of Adduct Formation in the Photochemical Reaction of 1,2,3,4-Tetramethylbenzene and Tetranitromethane

Acta Chemica Scandinavica, 1995

Research paper thumbnail of (4-Dimethylaminopyridine)5(benzoic acid)3(H2O)10: a two-dimensional clathrate hydrate

Journal of the Chemical Society, 1995

ABSTRACT

Research paper thumbnail of Synthesis of a cis-conformationally restricted peptide bond isostere and its application to the inhibition of the HIV-1 protease

Journal of the Chemical Society, 1997

Research paper thumbnail of Biologically active peptide analogues

Biologically active peptides and their receptors are involved in many of life's essential process... more Biologically active peptides and their receptors are involved in many of life's essential processes. They are of enormous medical interest as many diseases and illnesses can be treated by agents which block or imitate the function of specific peptides and their receptors. ' 11

Research paper thumbnail of Synthesis of a cis-conformationally restricted peptide bond isostere and its application to the inhibition of the HIV-1 protease

Journal of the Chemical Society, Perkin Transactions 1, 1997

Research paper thumbnail of Chemistry of the mycalamides: antiviral and antitumour compounds from a New Zealand marine sponge. Part 6.1–3 The synthesis and testing of analogues of the C(7)–C(10) fragment

Journal of the Chemical Society, Perkin Transactions 1, 1997

Research paper thumbnail of Protein-DNA Binding Affinities by Capillary Electrophoresis

Capillary Electrophoresis of Nucleic Acids

ABSTRACT Described in this chapter is a capillary electrophoresis (CE) method for the accurate de... more ABSTRACT Described in this chapter is a capillary electrophoresis (CE) method for the accurate determination of protein-DNA binding constants (1). The advantages of the method include the fast separation and quantitation of protein-DNA complex and free DNA, in uncoated capillaries without sieving matrices, coupled with LIF detection of fluorescently-labeled DNA at nM to πM concentrations.

Research paper thumbnail of Adduct Formation in the Photochemical Reaction of 1,2,3,4-Tetramethylbenzene and Tetranitromethane

Acta Chemica Scandinavica, 1995

Research paper thumbnail of (4-Dimethylaminopyridine)5(benzoic acid)3(H 2O)10: A two-dimensional clathrate hydrate

Research paper thumbnail of Photoaptamer arrays applied to multiplexed proteomic analysis

PROTEOMICS, 2004

Multiplexed photoaptamer‐based arrays that allow for the simultaneous measurement of multiple pro... more Multiplexed photoaptamer‐based arrays that allow for the simultaneous measurement of multiple proteins of interest in serum samples are described. Since photoaptamers covalently bind to their target analytes before fluorescent signal detection, the arrays can be vigorously washed to remove background proteins, providing the potential for superior signal‐to‐noise ratios and lower limits of quantification in biological matrices. Data are presented here for a 17‐plex photoaptamer array exhibiting limits of detection below 10 fM for several analytes including interleukin‐16, vascular endothelial growth factor, and endostatin and able to measure proteins in 10% serum samples. The assays are simple, scalable, and reproducible. Affinity of the capture reagent is shown to be directly correlated to the limit of detection for the analyte on the array.

Research paper thumbnail of DNA-binding affinities of MyoD and E47 homo- and hetero-dimers by capillary electrophoresis mobility shift assay

Journal of Chromatography A, 1999

A simple capillary electrophoresis mobility shift assay (CEMSA), with no gel and uncoated capilla... more A simple capillary electrophoresis mobility shift assay (CEMSA), with no gel and uncoated capillaries, for the accurate determination of protein-DNA affinities free in solution was applied to constructs of the MyoD/ E47 DNA-binding proteins. The determined affinities are compared to those obtained by EMSA. MyoD-E47 covalent heterodimer binds DNA more tightly (K 51.8 nM) than MyoD (K 514.2 nM) or E47 (K 511.5 nM) covalent homodimers. The effect of non-specific d d d DNA on binding affinities was more important than salt concentration in the MyoD/ E47 series. Application of this method to the MyoD/ E47 system demonstrates the generality of our CEMSA.

Research paper thumbnail of A capillary electrophoresis mobility shift assay for protein-DNA binding affinities free in solution

Nucleic Acids Research, 1998

Quantitative determination of dissociation constants for DNA-protein complexes will help clarify ... more Quantitative determination of dissociation constants for DNA-protein complexes will help clarify the molecular mechanisms of transcription, replication and DNA repair. A practical capillary electrophoresis mobility shift assay (CEMSA) for protein-DNA affinities free in solution is presented. The method is fast and simple, precise and general. The speed (<2 min separations) and simplicity derive from the use of an uncoated capillary with no gel matrix. The dissociation constant for GCNK58, a DNA-binding-region construct of the yeast transcription factor GCN4, binding to the AP1 DNA site was measured (K d = 35 ± 4 nM) to demonstrate the utility of the method.

Research paper thumbnail of Photoaptamer arrays applied to multiplexed proteomic analysis

Proteomics, Mar 1, 2004

Multiplexed photoaptamer‐based arrays that allow for the simultaneous measurement of multiple pro... more Multiplexed photoaptamer‐based arrays that allow for the simultaneous measurement of multiple proteins of interest in serum samples are described. Since photoaptamers covalently bind to their target analytes before fluorescent signal detection, the arrays can be vigorously washed to remove background proteins, providing the potential for superior signal‐to‐noise ratios and lower limits of quantification in biological matrices. Data are presented here for a 17‐plex photoaptamer array exhibiting limits of detection below 10 fM for several analytes including interleukin‐16, vascular endothelial growth factor, and endostatin and able to measure proteins in 10% serum samples. The assays are simple, scalable, and reproducible. Affinity of the capture reagent is shown to be directly correlated to the limit of detection for the analyte on the array.

Research paper thumbnail of Protein-DNA Binding Affinities by Capillary Electrophoresis

Humana Press eBooks, Nov 15, 2003

ABSTRACT Described in this chapter is a capillary electrophoresis (CE) method for the accurate de... more ABSTRACT Described in this chapter is a capillary electrophoresis (CE) method for the accurate determination of protein-DNA binding constants (1). The advantages of the method include the fast separation and quantitation of protein-DNA complex and free DNA, in uncoated capillaries without sieving matrices, coupled with LIF detection of fluorescently-labeled DNA at nM to πM concentrations.

Research paper thumbnail of Chemistry of the mycalamides: antiviral and antitumour compounds from a New Zealand marine sponge. Part 6.1–3 The synthesis and testing of analogues of the C(7)–C(10) fragment

Journal of the Chemical Society, 1997

Research paper thumbnail of Adduct Formation in the Photochemical Reaction of 1,2,3,4-Tetramethylbenzene and Tetranitromethane

Acta Chemica Scandinavica, 1995

Research paper thumbnail of (4-Dimethylaminopyridine)5(benzoic acid)3(H2O)10: a two-dimensional clathrate hydrate

Journal of the Chemical Society, 1995

ABSTRACT

Research paper thumbnail of Synthesis of a cis-conformationally restricted peptide bond isostere and its application to the inhibition of the HIV-1 protease

Journal of the Chemical Society, 1997

Research paper thumbnail of Biologically active peptide analogues

Biologically active peptides and their receptors are involved in many of life's essential process... more Biologically active peptides and their receptors are involved in many of life's essential processes. They are of enormous medical interest as many diseases and illnesses can be treated by agents which block or imitate the function of specific peptides and their receptors. ' 11

Research paper thumbnail of Synthesis of a cis-conformationally restricted peptide bond isostere and its application to the inhibition of the HIV-1 protease

Journal of the Chemical Society, Perkin Transactions 1, 1997

Research paper thumbnail of Chemistry of the mycalamides: antiviral and antitumour compounds from a New Zealand marine sponge. Part 6.1–3 The synthesis and testing of analogues of the C(7)–C(10) fragment

Journal of the Chemical Society, Perkin Transactions 1, 1997

Research paper thumbnail of Protein-DNA Binding Affinities by Capillary Electrophoresis

Capillary Electrophoresis of Nucleic Acids

ABSTRACT Described in this chapter is a capillary electrophoresis (CE) method for the accurate de... more ABSTRACT Described in this chapter is a capillary electrophoresis (CE) method for the accurate determination of protein-DNA binding constants (1). The advantages of the method include the fast separation and quantitation of protein-DNA complex and free DNA, in uncoated capillaries without sieving matrices, coupled with LIF detection of fluorescently-labeled DNA at nM to πM concentrations.

Research paper thumbnail of Adduct Formation in the Photochemical Reaction of 1,2,3,4-Tetramethylbenzene and Tetranitromethane

Acta Chemica Scandinavica, 1995

Research paper thumbnail of (4-Dimethylaminopyridine)5(benzoic acid)3(H 2O)10: A two-dimensional clathrate hydrate

Research paper thumbnail of Photoaptamer arrays applied to multiplexed proteomic analysis

PROTEOMICS, 2004

Multiplexed photoaptamer‐based arrays that allow for the simultaneous measurement of multiple pro... more Multiplexed photoaptamer‐based arrays that allow for the simultaneous measurement of multiple proteins of interest in serum samples are described. Since photoaptamers covalently bind to their target analytes before fluorescent signal detection, the arrays can be vigorously washed to remove background proteins, providing the potential for superior signal‐to‐noise ratios and lower limits of quantification in biological matrices. Data are presented here for a 17‐plex photoaptamer array exhibiting limits of detection below 10 fM for several analytes including interleukin‐16, vascular endothelial growth factor, and endostatin and able to measure proteins in 10% serum samples. The assays are simple, scalable, and reproducible. Affinity of the capture reagent is shown to be directly correlated to the limit of detection for the analyte on the array.

Research paper thumbnail of DNA-binding affinities of MyoD and E47 homo- and hetero-dimers by capillary electrophoresis mobility shift assay

Journal of Chromatography A, 1999

A simple capillary electrophoresis mobility shift assay (CEMSA), with no gel and uncoated capilla... more A simple capillary electrophoresis mobility shift assay (CEMSA), with no gel and uncoated capillaries, for the accurate determination of protein-DNA affinities free in solution was applied to constructs of the MyoD/ E47 DNA-binding proteins. The determined affinities are compared to those obtained by EMSA. MyoD-E47 covalent heterodimer binds DNA more tightly (K 51.8 nM) than MyoD (K 514.2 nM) or E47 (K 511.5 nM) covalent homodimers. The effect of non-specific d d d DNA on binding affinities was more important than salt concentration in the MyoD/ E47 series. Application of this method to the MyoD/ E47 system demonstrates the generality of our CEMSA.

Research paper thumbnail of A capillary electrophoresis mobility shift assay for protein-DNA binding affinities free in solution

Nucleic Acids Research, 1998

Quantitative determination of dissociation constants for DNA-protein complexes will help clarify ... more Quantitative determination of dissociation constants for DNA-protein complexes will help clarify the molecular mechanisms of transcription, replication and DNA repair. A practical capillary electrophoresis mobility shift assay (CEMSA) for protein-DNA affinities free in solution is presented. The method is fast and simple, precise and general. The speed (<2 min separations) and simplicity derive from the use of an uncoated capillary with no gel matrix. The dissociation constant for GCNK58, a DNA-binding-region construct of the yeast transcription factor GCN4, binding to the AP1 DNA site was measured (K d = 35 ± 4 nM) to demonstrate the utility of the method.