Francisco Javier Corpas - Academia.edu (original) (raw)

Papers by Francisco Javier Corpas

Nitrogen

In higher plants, hydrogen sulfide (H2S) is a recognized signaling molecule that performs multipl... more In higher plants, hydrogen sulfide (H2S) is a recognized signaling molecule that performs multiple regulatory functions. The enzyme L-cysteine desulfhydrase (LCD) catalyzes the conversion of L-cysteine (L-Cys) to pyruvate and ammonium with the concomitant generation of H₂S, and it is considered one of the main sources of H2S in plants. Using non-denaturing polyacrylamide gel electrophoresis (PAGE) in combination with a specific assay for LCD activity, this study aims to identify the potential LCD isozymes in wild-type Arabidopsis thaliana seedlings of 16 days old grown under in vitro conditions, and to evaluate the potential impact of nitric oxide (NO) and H2S on these LCD isozymes. For this purpose, an Atnoa1 mutant characterized to have a low endogenous NO content as well as the exogenous application of H2S were used. Five LCD isozymes were detected, with LCD IV being the isozyme that has the highest activity. However, the LCD V activity was the only one that was positively modula...

Progress in Botany, 2016

Nitric oxide ( · NO) and derived molecules, referred to as reactive nitrogen species (RNS), have ... more Nitric oxide ( · NO) and derived molecules, referred to as reactive nitrogen species (RNS), have become a new area of plant research. These molecules are involved in almost all physiological plant processes, ranging from seed germination, development, senescence, stomatal movement, fruit ripening, and reproduction to mechanisms of response to adverse environmental conditions possibly associated with nitro-oxidative stress. · NO can perform a dual function depending on its rate of production; at low concentrations, it acts as a signal molecule and, at high concentrations, like a stress molecule. Although in some cases the simultaneous high · NO production with other reactive oxygen species (ROS) can be useful to the cells as mechanism of defense, for example, against pathogens. All these processes are usually mediated by the chemical interactions of · NO whose functions are affected by other molecules. It is worth pointing out that the post-translational modifications of target proteins caused by nitration and S-nitrosylation have been best described in plants. However, · NO can also regulate gene expression through direct interaction with DNA or through interaction with transcription factors. This review provides a comprehensive overview of the role played by RNS in the physiology of plants and their involvement in the mechanism of response to a diverse range of adverse environmental conditions.

NADPH is an indispensable cofactor in a wide range of physiological processes which is generated ... more NADPH is an indispensable cofactor in a wide range of physiological processes which is generated by a family of NADPH-dehydrogenases, where the NADP-dependent malic enzyme (NADP-ME) is one member of these enzymes. Pepper (Capsicum annuum L.) fruit is a horticultural product worldwide consumed which has great nutritional and economic relevance. Besides the phenotypical changes that undergo pepper fruit during ripening, there are many associated modifications at transcriptomic, proteome ic, biochemical, and metabolic levels. Nitric oxide (NO) is a recognized signal molecule that can exert regulatory functions in diverse plant processes. To our knowledge, there is very scarce information about the number of genes encoding for NADP-ME in pepper plants and their expression during the ripening of sweet pepper fruit. Based on a data-mining approach on the pepper plant genome and fruit transcriptome (RNA-seq), five NADP-ME genes were identified, and four of them, namely CaNADP-ME2 to CaNADP...

Antioxidants, 2021

Excess selenium (Se) causes toxicity, and nitric oxide (NO)’s function in spermine (Spm)-induced ... more Excess selenium (Se) causes toxicity, and nitric oxide (NO)’s function in spermine (Spm)-induced tolerance to Se stress is unknown. Using wheat plants exposed to 1 mM sodium selenate—alone or in combination with either 1 mM Spm, 0.1 mM NO donor sodium nitroprusside (SNP) or 0.1 mM NO scavenger cPTIO—the potential beneficial effects of these compounds to palliate Se-induced stress were evaluated at physiological, biochemical and molecular levels. Se-treated plants accumulated Se in their roots (92%) and leaves (95%) more than control plants. Furthermore, Se diminished plant growth, photosynthetic traits and the relative water content and increased the levels of malondialdehyde, H2O2, osmolyte and endogenous NO. Exogenous Spm significantly decreased the levels of malondialdehyde by 28%, H2O2 by 37% and electrolyte leakage by 42%. Combined Spm/NO treatment reduced the Se content and triggered plant growth, photosynthetic traits, antioxidant enzymes and glyoxalase systems. Spm/NO also u...

Plant and Cell Physiology, 2021

Melatonin, a tryptophan-derived molecule, is endogenously generated in animal, plant, fungal and ... more Melatonin, a tryptophan-derived molecule, is endogenously generated in animal, plant, fungal and prokaryotic cells. Given its antioxidant properties, it is involved in a myriad of signaling functions associated with various aspects of plant growth and development. In higher plants, melatonin (Mel) interacts with plant regulators such as phytohormones, as well as reactive oxygen and nitrogen species including hydrogen peroxide (H2O2), nitric oxide (NO) and hydrogen sulfide (H2S). It shows great potential as a biotechnological tool to alleviate biotic and abiotic stress, to delay senescence and to conserve the sensory and nutritional quality of postharvest horticultural products which are of considerable economic importance worldwide. This review provides a comprehensive overview of the biochemistry of Mel, whose endogenous induction and exogenous application can play an important biotechnological role in enhancing the marketability and hence earnings from postharvest horticultural cr...

Plant Physiology and Biochemistry, 2021

Plant Signaling & Behavior, 2018

Peroxisome organelles have a versatile metabolism whose enzymatic content can be modulated by phy... more Peroxisome organelles have a versatile metabolism whose enzymatic content can be modulated by physiological and environment-dependent cellular conditions. They are characterized by a highly active nitro-oxidative metabolism and basic elements (H 2 O 2 and nitric oxide (NO)) with signaling properties. However, new elements have increased our understanding of the connections between peroxisomes and other cellular compartments. Furthermore, the presence of calcium (Ca 2C) intensifies communication between different signaling molecules and the relationship of Ca 2C itself with NO and H 2 O 2 .

Antioxidants, 2019

Low temperature (LT) negatively affects plant growth and development via the alteration of the me... more Low temperature (LT) negatively affects plant growth and development via the alteration of the metabolism of reactive oxygen and nitrogen species (ROS and RNS). Among RNS, tyrosine nitration, the addition of an NO2 group to a tyrosine residue, can modulate reduced nicotinamide-dinucleotide phosphate (NADPH)-generating systems and, therefore, can alter the levels of NADPH, a key cofactor in cellular redox homeostasis. NADPH also acts as an indispensable electron donor within a wide range of enzymatic reactions, biosynthetic pathways, and detoxification processes, which could affect plant viability. To extend our knowledge about the regulation of this key cofactor by this nitric oxide (NO)-related post-translational modification, we analyzed the effect of tyrosine nitration on another NADPH-generating enzyme, the NADP-malic enzyme (NADP-ME), under LT stress. In Arabidopsis thaliana seedlings exposed to short-term LT (4 °C for 48 h), a 50% growth reduction accompanied by an increase in...

Free Radical Biology and Medicine, 2018

Growth and maintenance of cells is partly controlled by the production and perception of a range ... more Growth and maintenance of cells is partly controlled by the production and perception of a range of small relatively reactive molecules, including reactive oxygen species, nitric oxide and hydrogen sulfide: what may be dubbed reactive signaling molecules (RSM). These compounds are generated by enzymes or arrive from the cell's environment, often produced in response to cellular stress, including temperature, salt or drought. Regularly studied on an individual basis, such compounds are often in cells at the same time and so interactions between them need to be considered. RSM alter antioxidants in cells, possibly leading to oxidative stress and cell death, perhaps through apoptotic pathways. Gene expression can alter, with the suite of genes regulated being similar for the different RSM. In reality the total cellular response will be the sum of interactions and perception of all RSM. RSM will influence the internal cellular redox poise and compete for thiols, the final modifications of which may determine the response. Lastly, other small molecules with potentially signaling roles need to be considered, e.g. hydrogen gas, which too can influence antioxidant levels in cells. Therefore, a holistic approach needs to be taken to understand the overall cellular response to RSM.

$Research paper thumbnail of {"__content__"=>"A Shoot Fe Signaling Pathway Requiring the OPT3 Transporter Controls GSNO Reductase and Ethylene in Roots.", "i"=>{"__content__"=>"Arabidopsis thaliana"}}$

Frontiers in plant science, 2018

Ethylene, nitric oxide (NO) and glutathione (GSH) increase in Fe-deficient roots of Strategy I sp... more Ethylene, nitric oxide (NO) and glutathione (GSH) increase in Fe-deficient roots of Strategy I species where they participate in the up-regulation of Fe acquisition genes. However, -nitrosoglutathione (GSNO), derived from NO and GSH, decreases in Fe-deficient roots. GSNO content is regulated by the GSNO-degrading enzyme -nitrosoglutathione reductase (GSNOR). On the other hand, there are several results showing that the regulation of Fe acquisition genes does not solely depend on hormones and signaling molecules (such as ethylene or NO), which would act as activators, but also on the internal Fe content of plants, which would act as a repressor. Moreover, different results suggest that total Fe in roots is not the repressor of Fe acquisition genes, but rather the repressor is a Fe signal that moves from shoots to roots through the phloem [hereafter named LOng Distance Iron Signal (LODIS)]. To look further in the possible interactions between LODIS, ethylene and GSNOR, we compared WT ...

Journal of plant physiology, Jan 15, 2016

NADPH is an important cofactor in cell growth, proliferation and detoxification. Arabidopsis thal... more NADPH is an important cofactor in cell growth, proliferation and detoxification. Arabidopsis thaliana Nudix hydrolase 19 (AtNUDX19) belongs to a family of proteins defined by the conserved amino-acid sequence GX5-EX7REUXEEXGU which has the capacity to hydrolyze NADPH as a physiological substrate in vivo. Given the importance of NADPH in the cellular redox homeostasis of plants, the present study compares the responses of the main NADPH-recycling systems including NADP-isocitrate dehydrogenase (ICDH), glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphogluconate dehydrogenase (6PGDH) and NADP-malic enzyme (ME) in the leaves and roots of Arabidopsis wild-type (Wt) and knock-out (KO) AtNUDX19 mutant (Atnudx19) plants under physiological and arsenic-induced stress conditions. Two major features were observed in the behavior of the main NADPH-recycling systems: (i) under optimal conditions in both organs, the levels of these activities were higher in nudx19 mutants than in Wt plants; and...

Scientifica, 2016

NADPH, whose regeneration is critical for reductive biosynthesis and detoxification pathways, is ... more NADPH, whose regeneration is critical for reductive biosynthesis and detoxification pathways, is an essential component in cell redox homeostasis. Peroxisomes are subcellular organelles with a complex biochemical machinery involved in signaling and stress processes by molecules such as hydrogen peroxide (H2O2) and nitric oxide (NO). NADPH is required by several peroxisomal enzymes involved inβ-oxidation, NO, and glutathione (GSH) generation. Plants have various NADPH-generating dehydrogenases, one of which is 6-phosphogluconate dehydrogenase (6PGDH). Arabidopsis contains three6PGDHgenes that probably are encoded for cytosolic, chloroplastic/mitochondrial, and peroxisomal isozymes, although their specific functions remain largely unknown. This study focuses on thein silicoanalysis of the biochemical characteristics and gene expression of peroxisomal 6PGDH (p6PGDH) with the aim of understanding its potential function in the peroxisomal NADPH-recycling system. The data show that a grou...

Plant Biology, 2015

Plant peroxisomes are unusual subcellular compartments with an apparent simple morphology but wit... more Plant peroxisomes are unusual subcellular compartments with an apparent simple morphology but with complex metabolic activity. The presence of signal molecules, such as hydrogen peroxide (H2 O2 ) and nitric oxide inside plant peroxisomes have added new functions in the cross-talk events among organelles and cells under physiological and stress conditions. Moreover, recent advances in proteomic analyses of plant peroxisomes have identified new protein candidates involved in several novel metabolic pathways. With all these new data, the present concise manuscript will focus on the relevance of the peroxisomal H2 O2 and its two main antioxidant enzymes, catalase and membrane-bound ascorbate peroxidase, which regulate its level and consequently its potential functions.

Ascorbate-Glutathione Pathway and Stress Tolerance in Plants, 2010

... DHAR and GR activities Palma et al. 2006 Barley (Hordeum vulgare) Heat, salt, and abscisic ac... more ... DHAR and GR activities Palma et al. 2006 Barley (Hordeum vulgare) Heat, salt, and abscisic acid treatments Increase in the transcript level of APX Shi et al. 2001 Grey mangrove (Avicennia marina) Light stress (500 mE m−2 s−1), Increase in the transcript level of APX Kavitha ...

Nature Plants, 2015

Breakthrough technologies to study living cells at the subcellular scale reveal that light modula... more Breakthrough technologies to study living cells at the subcellular scale reveal that light modulates the dynamic and reversible morphological adaptation of peroxisomes to optimize metabolic exchanges with chloroplasts during photorespiration.

Revista de Metalurgia, 2014

Se ha estudiado el acero inoxidable pulvimetalúrgico AISI 430L, comparando la sinterización en do... more Se ha estudiado el acero inoxidable pulvimetalúrgico AISI 430L, comparando la sinterización en dos atmósferas diferentes; en vacío, y en una atmósfera que contiene nitrógeno. Se ha desarrollado un tratamiento térmico con objeto de incrementar las propiedades mecánicas, mediante la modificación microestructural de los nitruros complejos de hierro y cromo precipitados durante la etapa de sinterización. Se han evaluado las propiedades físicas y a la vez se ha realizado un análisis microestructural con el fin de relacionar la microestructura con el incremento en las propiedades mecánicas.