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Papers by Frank Geeter
EJNMMI Research, 2014
Background: To combine the sensitivity of bioluminescent imaging (BLI) with the 3D and quantitati... more Background: To combine the sensitivity of bioluminescent imaging (BLI) with the 3D and quantitative properties of pinhole single-photon emission computed tomography (SPECT)/micro-computed tomography (CT) (phSPECT/micro-CT), we generated stable cell lines that express a yellow-fluorescent protein (YFP) and Gaussia luciferase (GLuc) fusion protein (YFP/GLuc). For in vivo phSPECT detection of this YFP/GLuc protein, a nanobody, targeted against yellow and green fluorescent proteins (anti-YFP-Nb), was site specifically labelled with 99m Tc.
European Journal of Nuclear Medicine and Molecular Imaging, 2011
Contrast Media & Molecular Imaging, 2009
In several disease models it is known that heterogeneous dysinnervation occurs in the sympathetic... more In several disease models it is known that heterogeneous dysinnervation occurs in the sympathetic nervous system. We therefore adapted the (123)I-MIBG imaging procedure in small animals to allow quantification of both global and regional uptake and wash-out rate using high specific activity (123)I-MIBG in the myocardium of rats. After evaluation of the image procedure in normal animals, we then applied our imaging protocol to visualize the regional dysinnervation in cardiac autonomic neuropathy occurring in streptozotocin-induced diabetes. Seven normal Lewis rats underwent (123)I-MIBG pinhole SPECT with low specific activity (123)I-MIBG (lsa MIBG) and high specific activity (123)I-MIBG (hsa MIBG) with a 2 week interval. Twelve normal rats and 12 rats 8 weeks after streptozotocin injection underwent the same hsa MIBG imaging protocol. The imaging protocol consisted of two SPECT acquisitions for every animal. The imaging sequence started at 20 min after tracer injection. The percentage of injected activity (%IA) and the wash-out rate in the global myocardium were measured. Left ventricular regional MIBG kinetics were analyzed in the six midventricular segments of the 17 segment model. Compared with lsa MIBG, the wash-out rate of hsa MIBG was significantly slower, in association with a higher cardiac uptake. Regional analysis showed a maximal uptake in the anterolateral segment, without significant differences between segments. We noted a significantly higher global wash-out rate in the streptozotocin group compared to controls (p < 0.05). Regional analysis confirmed the increased wash-out rate, reaching statistical significance in the inferior and the inferoseptal walls. High-quality (123)I-MIBG images and accurate measurements can be obtained using hsa (123)I-MIBG with image acquisitions performed at relatively early time points. Small animal MIBG SPECT imaging allows for regional analysis of the myocardium. In the streptozotocin group, wash-out of MIBG is globally increased, compatible with a higher sympathetic tonus or decreased reuptake of MIBG. The highest increase is located in the inferior, inferoseptal and anteroseptal walls. These findings further suggest the occurrence of diabetic cardiomyopthy after streptozotocin injection.
Clinical Nuclear Medicine, 1995
ABSTRACT
Accreditation and Quality Assurance, 2004
... B. Wuyts (✉) · D. Bernard · N. Van Den Noortgate · J. Van De Walle · B. Van Vlem · R. De Smet... more ... B. Wuyts (✉) · D. Bernard · N. Van Den Noortgate · J. Van De Walle · B. Van Vlem · R. De Smet · R. Vanholder · J. Delanghe ... Nuclear Medicine, AZ St Jan, Ruddershove 10, B8000 Brugge, Belgium J.-C. Libeer Institute of Public Health Louis Pasteur, Juliette Wytmanstraat 14 ...
EJNMMI Research, 2014
Background: To combine the sensitivity of bioluminescent imaging (BLI) with the 3D and quantitati... more Background: To combine the sensitivity of bioluminescent imaging (BLI) with the 3D and quantitative properties of pinhole single-photon emission computed tomography (SPECT)/micro-computed tomography (CT) (phSPECT/micro-CT), we generated stable cell lines that express a yellow-fluorescent protein (YFP) and Gaussia luciferase (GLuc) fusion protein (YFP/GLuc). For in vivo phSPECT detection of this YFP/GLuc protein, a nanobody, targeted against yellow and green fluorescent proteins (anti-YFP-Nb), was site specifically labelled with 99m Tc.
European Journal of Nuclear Medicine and Molecular Imaging, 2011
Contrast Media & Molecular Imaging, 2009
In several disease models it is known that heterogeneous dysinnervation occurs in the sympathetic... more In several disease models it is known that heterogeneous dysinnervation occurs in the sympathetic nervous system. We therefore adapted the (123)I-MIBG imaging procedure in small animals to allow quantification of both global and regional uptake and wash-out rate using high specific activity (123)I-MIBG in the myocardium of rats. After evaluation of the image procedure in normal animals, we then applied our imaging protocol to visualize the regional dysinnervation in cardiac autonomic neuropathy occurring in streptozotocin-induced diabetes. Seven normal Lewis rats underwent (123)I-MIBG pinhole SPECT with low specific activity (123)I-MIBG (lsa MIBG) and high specific activity (123)I-MIBG (hsa MIBG) with a 2 week interval. Twelve normal rats and 12 rats 8 weeks after streptozotocin injection underwent the same hsa MIBG imaging protocol. The imaging protocol consisted of two SPECT acquisitions for every animal. The imaging sequence started at 20 min after tracer injection. The percentage of injected activity (%IA) and the wash-out rate in the global myocardium were measured. Left ventricular regional MIBG kinetics were analyzed in the six midventricular segments of the 17 segment model. Compared with lsa MIBG, the wash-out rate of hsa MIBG was significantly slower, in association with a higher cardiac uptake. Regional analysis showed a maximal uptake in the anterolateral segment, without significant differences between segments. We noted a significantly higher global wash-out rate in the streptozotocin group compared to controls (p < 0.05). Regional analysis confirmed the increased wash-out rate, reaching statistical significance in the inferior and the inferoseptal walls. High-quality (123)I-MIBG images and accurate measurements can be obtained using hsa (123)I-MIBG with image acquisitions performed at relatively early time points. Small animal MIBG SPECT imaging allows for regional analysis of the myocardium. In the streptozotocin group, wash-out of MIBG is globally increased, compatible with a higher sympathetic tonus or decreased reuptake of MIBG. The highest increase is located in the inferior, inferoseptal and anteroseptal walls. These findings further suggest the occurrence of diabetic cardiomyopthy after streptozotocin injection.
Clinical Nuclear Medicine, 1995
ABSTRACT
Accreditation and Quality Assurance, 2004
... B. Wuyts (✉) · D. Bernard · N. Van Den Noortgate · J. Van De Walle · B. Van Vlem · R. De Smet... more ... B. Wuyts (✉) · D. Bernard · N. Van Den Noortgate · J. Van De Walle · B. Van Vlem · R. De Smet · R. Vanholder · J. Delanghe ... Nuclear Medicine, AZ St Jan, Ruddershove 10, B8000 Brugge, Belgium J.-C. Libeer Institute of Public Health Louis Pasteur, Juliette Wytmanstraat 14 ...