G. Kardar - Academia.edu (original) (raw)

Papers by G. Kardar

Research paper thumbnail of P4.05 * The study of CD8+ T cell response to MHC class I-restricted universal tumoral peptide in accompany with viral peptide

Annals of Oncology

Breast cancers likes as other human cancers are immunogenic and express tumor-associated proteins... more Breast cancers likes as other human cancers are immunogenic and express tumor-associated proteins that can stimulate and generate immune responses in cancer. One of the important antigen that is express in tumors especially in Brest cancers is human Telomerase reverse transcriptase (hTERT). There are some databases that store the information about T cell epitopes, and we used them to identify immunogenic epitopes in proteins that bind in Class I MHC. in this study we used a peptide derived of hTERT molecule accompany with viral peptide derived of influenza that restricted to HLA-A2 (common MHC class I in Iran) for induction of T CD8+ responses in breast cancer patients. PBMCs were obtained from blood sample of HLA-A2 positive breast cancer patient incubated with tumor cell line that were transloaded with synthetic peptides from hTERT and viral peptides. Proliferation of T cells analyzed with MTT reaction. After this step, supernatants from previous step evaluated for IFN-γ productio...

Research paper thumbnail of Cytokine production by activated T cells in common variable immunodeficiency

Journal of investigational allergology & clinical immunology, 2010

Common variable immunodeficiency (CVID) is the most common symptomatic antibody deficiency. It is... more Common variable immunodeficiency (CVID) is the most common symptomatic antibody deficiency. It is characterized by hypogammaglobulinemia, increased susceptibility to recurrent infections, autoimmunity, and malignancies. To determine whether patients with CVID have cytokine production defects after T-cell activation and to assess whether or not these are correlated with markers of severe disease. Twenty-seven patients with CVID and 17 healthy volunteers were investigated. Peripheral blood mononuclear cells were cultured under standard conditions in the presence and absence of phytohemagglutinin. Subsequent cell proliferation and cytokine release were measured and compared between stimulated and unstimulated cells. A general enhancement in cytokine production was observed in both CVID patients and controls after stimulation. However, we detected a lower production of interferon-gamma in CVID patients than in controls (P = .026). A production defect for at least 1 cytokine was observed...

Research paper thumbnail of T- helper 1 and 2 cytokine assay in patients with common variable immunodeficiency

Journal of investigational allergology & clinical immunology, 2008

Common variable immunodeficiency (CVID) is a heterogeneous group of disorders, characterized by d... more Common variable immunodeficiency (CVID) is a heterogeneous group of disorders, characterized by decreased immunoglobulin levels in serum and increased susceptibility to recurrent infections, autoimmunity, and malignancy. The pathogenesis of CVID is still unknown. This study was performed to investigate T-helper (T(H)) 1 and 2 cytokine levels in patients with CVID. Twenty-four cases of CVID were studied. Cytokine levels of interleukin (IL)-2, IL-4, IL-10, and interferon gamma were measured in the serum of the patients and compared with those of healthy controls. T(H)2 cytokine levels (IL-4 and IL-10) were significantly higher in the patient group than in the controls (median: 64.5 vs 0.0 pg/mL, P=.016 for IL-4 and 321.1 vs. 0.0 pg/mL, P=.001 for IL-10). However, there were no significant differences in T(H)1 cytokines between the 2 groups (median, 116.5 vs. 104.5 pg/mL, P=.22 for IL-2 and 50.5 vs. 42.3 pg/mL, P=.32 for IFN-gamma). Increased levels of IL-4 and IL-10 could indicate hig...

Research paper thumbnail of Phylogenetic analyses of human alpha subunit of FCepsilonRI with other species

Research paper thumbnail of Influence of selenium on mast cells mediator release

Research paper thumbnail of Diminished Th1 and Th2 Cytokine Production in Healthy Adult Nonresponders to Recombinant Hepatitis B Vaccine

Scandinavian Journal of Immunology, 2002

Vaccination of healthy adults with recombinant hepatitis B (rHB) vaccine fails to induce a protec... more Vaccination of healthy adults with recombinant hepatitis B (rHB) vaccine fails to induce a protective antibody response in a proportion of individuals. Imbalanced T-helper (Th)1/Th2 response has been attributed to the lack of specific antibody response to rHB vaccine. In this study, in vitro production of interleukin (IL)-2, interferon (IFN)-g and IL-10 was investigated in Iranian healthy adults vaccinated with rHB vaccine. Peripheral blood mononuclear cells (PBMC) were isolated from 18 high responders and eight nonresponders and stimulated with rHB antigen or phytohaemaglutinin (PHA) mitogen. The cytokines were quantitated in culture supernatants by sandwich enzyme-linked immunosorbent assay (ELISA). Our results demonstrated a significant decrease in the production of IL-2, IFN-g and IL-10 (P < 0.005) in response to rHB antigen. The levels of all cytokines induced by PHA were similarly represented in both groups of vaccinees. These findings suggest that unresponsiveness to rHB vaccine may be owing to inadequate Th1-and Th2-like cytokine production.

Research paper thumbnail of Serum concentration of selenium in healthy individuals living in Tehran

Nutrition journal, 2005

To investigate whether daily diet provides adequate selenium intake in healthy men and women livi... more To investigate whether daily diet provides adequate selenium intake in healthy men and women living in Tehran, Iran. Serum level of selenium was determined in 184 healthy individuals of both genders. The samples were divided into two age groups, adults and children, for analysis. The serum level of selenium was determined using hydride generation and flame atomic absorption spectroscopy. The mean and standard deviation of serum selenium levels in children (1-16 years) was 84.3 +/- 11 microg/l and there was no significant difference between genders in this group. In adults (older than 16 years) the mean serum selenium level was 100.6 +/- 13 SD microg/l; among women the mean was 93.9 +/- 14 SD microg/l and among men it was 102.2 +/- 12 SD microg/l. The mean selenium level in men was higher than in women and data analysis showed a significant difference between them (p < 0.005). There was also a positive correlation between higher selenium serum concentration and age in men (P < ...

Research paper thumbnail of Total IgE level in different allergic diseases and healthy children in Iran

Research paper thumbnail of Phylogenetic analyses of human alpha subunit of FCepsilonRI with other species

Research paper thumbnail of Comparative study of Avicennia marina pollens allergenicity in two regions of Bushehre province in Iran

Research paper thumbnail of Normal Value of Immunoglobulins IgA, IgG, and IgM in Iranian Healthy Adults, Measured by Nephelometry

Journal of Immunoassay and Immunochemistry, 2003

Local reference ranges of immunoglobulins are required for studies and clinical interpretation. I... more Local reference ranges of immunoglobulins are required for studies and clinical interpretation. In this study, serum levels of IgA, IgG, and IgM in 914 Iranian healthy adult blood donor volunteers, aged 18-55 years, were measured by nephelometry. Our data showed that serum reference intervals of IgA, IgG, and IgM in subjects were 72-375, 636-1518, and 39-283 mg/dL, respectively. Data analysis showed a significant difference between the male and female subjects only for IgM; thus, the means of IgM in females were higher than for males (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05). Correlation coefficient r for paired samples showed no statistically significant relationship between age and each Ig (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt; 0.05). Comparison of this study with others demonstrated that results are similar; some differences are probably related to ethnic differences. Therefore, our results can be considered as a source of reliable local reference for use in laboratories.

Research paper thumbnail of A Laboratory Method for Purification of Major Cow's Milk Allergens

Journal of Immunoassay and Immunochemistry, 2004

Food allergy is responsible for the most frequent allergic reactions in children under 1 year of ... more Food allergy is responsible for the most frequent allergic reactions in children under 1 year of age. Diagnostic tests such as skin test or specific IgE assay usually need highly purified preparations of allergenic proteins. The aim of the present study was to purify three main cow&amp;amp;#39;s milk proteins: casein, alpha-lactalbumin, and beta-lactoglobulin as allergenic extracts for first time in Iran.

Research paper thumbnail of Correlation between serum zinc levels and successful immunotherapy in recurrent spontaneous abortion patients

Journal of Human Reproductive Sciences, 2013

Research paper thumbnail of Comparative study of Avicennia marina (Forsk.) Vierh. Pollens allergenicity in two regions of Bushehr province in Iran

Research paper thumbnail of Total IgE levels in healthy children in Tehran, Iran

Determining the normal range of Immunoglobulin E (IgE) in any population is important for diagnos... more Determining the normal range of Immunoglobulin E (IgE) in any population is important for diagnosis of allergic diseases. The aim of this study was to determine normal values of IgE in children living in Tehran. Two-handed and fifty six healthy children aged one month to ...

Research paper thumbnail of Prevalence of type I allergy to natural rubber latex and type IV allergy to latex and rubber additives in operating room staff with glove-related symptoms

Allergy and Asthma Proceedings, 2007

There is lack of data on the prevalence of latex allergy in the health care setting in Iran. This... more There is lack of data on the prevalence of latex allergy in the health care setting in Iran. This study was performed to determine the prevalence of type I latex allergy and type IV allergy to latex and rubber additives among the operating room staff with glove-related symptoms in 13 general hospitals in Tehran. Skin-prick tests with commercial latex extract, patch tests with latex and 25 rubber additive series, and total and latex-specific IgE detection were performed on the operating room staff who reported latex glove-related symptoms. Five hundred twelve self-administered questionnaires (100%) were completed by all operating room staff and latex glove-related symptoms were reported by 59 (11.5%) employees. Among all symptomatic operating room staff tested, the prevalence of type I latex allergy was 30.5% and the prevalence rates of type IV allergy to latex and rubber additives were 16.7 and 14.6%, respectively. The most positive patch test result with rubber additives was related to tetramethylthiuram monosulfide (38.5%). The risk factors for type I latex allergy were female sex (p = 0.009) and positive patch test with rubber additives (p = 0.012). Subjects who had positive patch test with latex were significantly more likely to have positive patch test with rubber additives (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.0001). Our results showed a high prevalence of type I latex allergy and type IV allergy to latex and rubber additives. Based on this study, we recommend eliminating powdered latex gloves from the operating rooms of the 13 studied general hospitals and support the substitution of powder-free latex gloves.

Research paper thumbnail of Selective immunoglobulin a deficiency in Iranian blood donors: prevalence, laboratory and clinical findings

Copyright© 2008, IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY. All rights reserved. ... ORIG... more Copyright© 2008, IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY. All rights reserved. ... ORIGINAL ARTICLE Iran J Allergy Asthma Immunol September 2008; 7(3): 157-162 ... Selective Immunoglobulin A Deficiency in Iranian Blood Donors: Prevalence, ...

Research paper thumbnail of Effect of environmental pollution on the proteins, allergenic bands, ontogeny and structure of Avicennia marina (Forsk.) Vierh (Avicenniaceae) pollen grains

Aerobiologia, 2014

ABSTRACT In Bushehr province of Iran, Avicennia marina trees have grown in Bordekhoon (Mond Prote... more ABSTRACT In Bushehr province of Iran, Avicennia marina trees have grown in Bordekhoon (Mond Protected Area) and Assaluyeh (Marine National Park of Nayband). Contrary to Bordekhoon, Assaluyeh is a petrochemical region with environmental pollution. This study was aimed to studying protein profiles, allergenic bands, ontogeny, structure and elemental composition of tectum of A. marina pollens in Assaluyeh and Bordekhoon. Pollens were collected from two regions and extracted in PBS, and protein profiles of pollens were determined by sulfate–polyacrylamide gel electrophoresis (SDS-PAGE). As an experimental model, 20 female 6–8-week-old Balb/C mice were divided into two groups. The mice of first and second groups were sensitized by Bordekhoon and Assaluyeh pollen extracts, respectively, and mice serum samples were used for immunoblotting. Pollen characteristics were studied using light and scanning electron microscopes. SDS-PAGE showed some differences between pollen protein profiles of two regions. Immunoblotting assay detected that pollens have two allergenic bands and the protein band at 100 KD is the common allergenic protein in two regions. Light microscopy revealed that the development of anther wall was basic type and some abnormalities were observed in microspores and pollens of Assaluyeh. Scanning electron microscopy studies showed that the apertures in considerable numbers of Assaluyeh pollens were closed. The comparison of elemental composition of pollen tectum between two regions showed that pollens of Assaluyeh have accumulated Cu on their tectum. Results obtained indicated that environmental pollution can affect protein profile, allergenic bands, structure and elemental composition of tectum of A. marina pollens.

Research paper thumbnail of Primary immunodeficiency (PP-051) PP-051-09 The immunological alteration and gene mutations in Combined ImmunodeficiencyPP-051-10 Swedish Guidelines for the assessment, diagnosis and management of 6 primary immunodeficiency states: CVID, IgG subclass deficiency, IgA deficiency, XLA, SCID and CGDP...

International Immunology Meeting Abstracts, Aug 1, 2010

In order to investigate the immunological features and characteristic of gene mutation in Chinese... more In order to investigate the immunological features and characteristic of gene mutation in Chinese patients with combined immunodeficiency, case histories were analyzed repeatedly to grasp important characteristics of the diseases. Distribution of lymphocyte subsets from peripheral blood and the level of CD40L expressing on activated CD3+ CD8− T lymphocyte surface were examined by flow cytometry. Amplify and identify exons from genes encoding CD40L and IL-2Rγc by PCR and agarose gel electrophoresis ...

Research paper thumbnail of Molecular analysis of the heavy chain variable region genes of human hybridoma clones specific for coagulation factor VIII

Thrombosis and Haemostasis, 2005

HemophiliaA is a X-linked hematologic disorder characterized by undetectable or low amounts of fu... more HemophiliaA is a X-linked hematologic disorder characterized by undetectable or low amounts of functional coagulation factor VIII (FVIII). Replacement therapy induces FVIII neutralizing antibody (Ab) (inhibitor) in a proportion of patients which makes further treatment of these patients ineffective and costly. To envisage mechanisms underlying inhibitor development, seven hybridoma clones specific for FVIII were generated from two hemophilia A patients with high titer of inhibitor. Specificity and isotype of the monoclonal antibodies (mAbs) were determined by ELISA. Immunoglobulin (Ig) variable region heavy (VH) chain gene family usage was identified by RT-PCR using VH1-6 specific primers. Nucleotide sequences of the VH gene of FVIII specific clones were determined and aligned to the most homologous germ line genes in the GenBank. Analysis of the expressed VH genes by RT-PCR revealed that the hybridomas utilized either the VH1 (71%) or the VH3 (29%) gene family. Three VH domains were encoded by V1-69 (DP-10),V1-2 (DP-8), and V1-8 (DP-15) genes and two by V1-18 (DP-14) gene, all from the VH1 gene family. Of theVH3-gene family expressing clones, one belonged to V3-66 (DP-86) and the other one to V3-21 (DP-77) germline genes. The CDR3 length was found to be highly different amongst these clones ranging from 11 to 22 amino acid residues. These data suggest that FVIII-specific Abs preferentially use VH gene segments derived from VH1 gene family. Diversity of the expressed VH genes and their CDR3 length implies that different epitopes are recognized by these mAbs.

Research paper thumbnail of P4.05 * The study of CD8+ T cell response to MHC class I-restricted universal tumoral peptide in accompany with viral peptide

Annals of Oncology

Breast cancers likes as other human cancers are immunogenic and express tumor-associated proteins... more Breast cancers likes as other human cancers are immunogenic and express tumor-associated proteins that can stimulate and generate immune responses in cancer. One of the important antigen that is express in tumors especially in Brest cancers is human Telomerase reverse transcriptase (hTERT). There are some databases that store the information about T cell epitopes, and we used them to identify immunogenic epitopes in proteins that bind in Class I MHC. in this study we used a peptide derived of hTERT molecule accompany with viral peptide derived of influenza that restricted to HLA-A2 (common MHC class I in Iran) for induction of T CD8+ responses in breast cancer patients. PBMCs were obtained from blood sample of HLA-A2 positive breast cancer patient incubated with tumor cell line that were transloaded with synthetic peptides from hTERT and viral peptides. Proliferation of T cells analyzed with MTT reaction. After this step, supernatants from previous step evaluated for IFN-γ productio...

Research paper thumbnail of Cytokine production by activated T cells in common variable immunodeficiency

Journal of investigational allergology & clinical immunology, 2010

Common variable immunodeficiency (CVID) is the most common symptomatic antibody deficiency. It is... more Common variable immunodeficiency (CVID) is the most common symptomatic antibody deficiency. It is characterized by hypogammaglobulinemia, increased susceptibility to recurrent infections, autoimmunity, and malignancies. To determine whether patients with CVID have cytokine production defects after T-cell activation and to assess whether or not these are correlated with markers of severe disease. Twenty-seven patients with CVID and 17 healthy volunteers were investigated. Peripheral blood mononuclear cells were cultured under standard conditions in the presence and absence of phytohemagglutinin. Subsequent cell proliferation and cytokine release were measured and compared between stimulated and unstimulated cells. A general enhancement in cytokine production was observed in both CVID patients and controls after stimulation. However, we detected a lower production of interferon-gamma in CVID patients than in controls (P = .026). A production defect for at least 1 cytokine was observed...

Research paper thumbnail of T- helper 1 and 2 cytokine assay in patients with common variable immunodeficiency

Journal of investigational allergology & clinical immunology, 2008

Common variable immunodeficiency (CVID) is a heterogeneous group of disorders, characterized by d... more Common variable immunodeficiency (CVID) is a heterogeneous group of disorders, characterized by decreased immunoglobulin levels in serum and increased susceptibility to recurrent infections, autoimmunity, and malignancy. The pathogenesis of CVID is still unknown. This study was performed to investigate T-helper (T(H)) 1 and 2 cytokine levels in patients with CVID. Twenty-four cases of CVID were studied. Cytokine levels of interleukin (IL)-2, IL-4, IL-10, and interferon gamma were measured in the serum of the patients and compared with those of healthy controls. T(H)2 cytokine levels (IL-4 and IL-10) were significantly higher in the patient group than in the controls (median: 64.5 vs 0.0 pg/mL, P=.016 for IL-4 and 321.1 vs. 0.0 pg/mL, P=.001 for IL-10). However, there were no significant differences in T(H)1 cytokines between the 2 groups (median, 116.5 vs. 104.5 pg/mL, P=.22 for IL-2 and 50.5 vs. 42.3 pg/mL, P=.32 for IFN-gamma). Increased levels of IL-4 and IL-10 could indicate hig...

Research paper thumbnail of Phylogenetic analyses of human alpha subunit of FCepsilonRI with other species

Research paper thumbnail of Influence of selenium on mast cells mediator release

Research paper thumbnail of Diminished Th1 and Th2 Cytokine Production in Healthy Adult Nonresponders to Recombinant Hepatitis B Vaccine

Scandinavian Journal of Immunology, 2002

Vaccination of healthy adults with recombinant hepatitis B (rHB) vaccine fails to induce a protec... more Vaccination of healthy adults with recombinant hepatitis B (rHB) vaccine fails to induce a protective antibody response in a proportion of individuals. Imbalanced T-helper (Th)1/Th2 response has been attributed to the lack of specific antibody response to rHB vaccine. In this study, in vitro production of interleukin (IL)-2, interferon (IFN)-g and IL-10 was investigated in Iranian healthy adults vaccinated with rHB vaccine. Peripheral blood mononuclear cells (PBMC) were isolated from 18 high responders and eight nonresponders and stimulated with rHB antigen or phytohaemaglutinin (PHA) mitogen. The cytokines were quantitated in culture supernatants by sandwich enzyme-linked immunosorbent assay (ELISA). Our results demonstrated a significant decrease in the production of IL-2, IFN-g and IL-10 (P < 0.005) in response to rHB antigen. The levels of all cytokines induced by PHA were similarly represented in both groups of vaccinees. These findings suggest that unresponsiveness to rHB vaccine may be owing to inadequate Th1-and Th2-like cytokine production.

Research paper thumbnail of Serum concentration of selenium in healthy individuals living in Tehran

Nutrition journal, 2005

To investigate whether daily diet provides adequate selenium intake in healthy men and women livi... more To investigate whether daily diet provides adequate selenium intake in healthy men and women living in Tehran, Iran. Serum level of selenium was determined in 184 healthy individuals of both genders. The samples were divided into two age groups, adults and children, for analysis. The serum level of selenium was determined using hydride generation and flame atomic absorption spectroscopy. The mean and standard deviation of serum selenium levels in children (1-16 years) was 84.3 +/- 11 microg/l and there was no significant difference between genders in this group. In adults (older than 16 years) the mean serum selenium level was 100.6 +/- 13 SD microg/l; among women the mean was 93.9 +/- 14 SD microg/l and among men it was 102.2 +/- 12 SD microg/l. The mean selenium level in men was higher than in women and data analysis showed a significant difference between them (p < 0.005). There was also a positive correlation between higher selenium serum concentration and age in men (P < ...

Research paper thumbnail of Total IgE level in different allergic diseases and healthy children in Iran

Research paper thumbnail of Phylogenetic analyses of human alpha subunit of FCepsilonRI with other species

Research paper thumbnail of Comparative study of Avicennia marina pollens allergenicity in two regions of Bushehre province in Iran

Research paper thumbnail of Normal Value of Immunoglobulins IgA, IgG, and IgM in Iranian Healthy Adults, Measured by Nephelometry

Journal of Immunoassay and Immunochemistry, 2003

Local reference ranges of immunoglobulins are required for studies and clinical interpretation. I... more Local reference ranges of immunoglobulins are required for studies and clinical interpretation. In this study, serum levels of IgA, IgG, and IgM in 914 Iranian healthy adult blood donor volunteers, aged 18-55 years, were measured by nephelometry. Our data showed that serum reference intervals of IgA, IgG, and IgM in subjects were 72-375, 636-1518, and 39-283 mg/dL, respectively. Data analysis showed a significant difference between the male and female subjects only for IgM; thus, the means of IgM in females were higher than for males (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05). Correlation coefficient r for paired samples showed no statistically significant relationship between age and each Ig (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt; 0.05). Comparison of this study with others demonstrated that results are similar; some differences are probably related to ethnic differences. Therefore, our results can be considered as a source of reliable local reference for use in laboratories.

Research paper thumbnail of A Laboratory Method for Purification of Major Cow's Milk Allergens

Journal of Immunoassay and Immunochemistry, 2004

Food allergy is responsible for the most frequent allergic reactions in children under 1 year of ... more Food allergy is responsible for the most frequent allergic reactions in children under 1 year of age. Diagnostic tests such as skin test or specific IgE assay usually need highly purified preparations of allergenic proteins. The aim of the present study was to purify three main cow&amp;amp;#39;s milk proteins: casein, alpha-lactalbumin, and beta-lactoglobulin as allergenic extracts for first time in Iran.

Research paper thumbnail of Correlation between serum zinc levels and successful immunotherapy in recurrent spontaneous abortion patients

Journal of Human Reproductive Sciences, 2013

Research paper thumbnail of Comparative study of Avicennia marina (Forsk.) Vierh. Pollens allergenicity in two regions of Bushehr province in Iran

Research paper thumbnail of Total IgE levels in healthy children in Tehran, Iran

Determining the normal range of Immunoglobulin E (IgE) in any population is important for diagnos... more Determining the normal range of Immunoglobulin E (IgE) in any population is important for diagnosis of allergic diseases. The aim of this study was to determine normal values of IgE in children living in Tehran. Two-handed and fifty six healthy children aged one month to ...

Research paper thumbnail of Prevalence of type I allergy to natural rubber latex and type IV allergy to latex and rubber additives in operating room staff with glove-related symptoms

Allergy and Asthma Proceedings, 2007

There is lack of data on the prevalence of latex allergy in the health care setting in Iran. This... more There is lack of data on the prevalence of latex allergy in the health care setting in Iran. This study was performed to determine the prevalence of type I latex allergy and type IV allergy to latex and rubber additives among the operating room staff with glove-related symptoms in 13 general hospitals in Tehran. Skin-prick tests with commercial latex extract, patch tests with latex and 25 rubber additive series, and total and latex-specific IgE detection were performed on the operating room staff who reported latex glove-related symptoms. Five hundred twelve self-administered questionnaires (100%) were completed by all operating room staff and latex glove-related symptoms were reported by 59 (11.5%) employees. Among all symptomatic operating room staff tested, the prevalence of type I latex allergy was 30.5% and the prevalence rates of type IV allergy to latex and rubber additives were 16.7 and 14.6%, respectively. The most positive patch test result with rubber additives was related to tetramethylthiuram monosulfide (38.5%). The risk factors for type I latex allergy were female sex (p = 0.009) and positive patch test with rubber additives (p = 0.012). Subjects who had positive patch test with latex were significantly more likely to have positive patch test with rubber additives (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.0001). Our results showed a high prevalence of type I latex allergy and type IV allergy to latex and rubber additives. Based on this study, we recommend eliminating powdered latex gloves from the operating rooms of the 13 studied general hospitals and support the substitution of powder-free latex gloves.

Research paper thumbnail of Selective immunoglobulin a deficiency in Iranian blood donors: prevalence, laboratory and clinical findings

Copyright© 2008, IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY. All rights reserved. ... ORIG... more Copyright© 2008, IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY. All rights reserved. ... ORIGINAL ARTICLE Iran J Allergy Asthma Immunol September 2008; 7(3): 157-162 ... Selective Immunoglobulin A Deficiency in Iranian Blood Donors: Prevalence, ...

Research paper thumbnail of Effect of environmental pollution on the proteins, allergenic bands, ontogeny and structure of Avicennia marina (Forsk.) Vierh (Avicenniaceae) pollen grains

Aerobiologia, 2014

ABSTRACT In Bushehr province of Iran, Avicennia marina trees have grown in Bordekhoon (Mond Prote... more ABSTRACT In Bushehr province of Iran, Avicennia marina trees have grown in Bordekhoon (Mond Protected Area) and Assaluyeh (Marine National Park of Nayband). Contrary to Bordekhoon, Assaluyeh is a petrochemical region with environmental pollution. This study was aimed to studying protein profiles, allergenic bands, ontogeny, structure and elemental composition of tectum of A. marina pollens in Assaluyeh and Bordekhoon. Pollens were collected from two regions and extracted in PBS, and protein profiles of pollens were determined by sulfate–polyacrylamide gel electrophoresis (SDS-PAGE). As an experimental model, 20 female 6–8-week-old Balb/C mice were divided into two groups. The mice of first and second groups were sensitized by Bordekhoon and Assaluyeh pollen extracts, respectively, and mice serum samples were used for immunoblotting. Pollen characteristics were studied using light and scanning electron microscopes. SDS-PAGE showed some differences between pollen protein profiles of two regions. Immunoblotting assay detected that pollens have two allergenic bands and the protein band at 100 KD is the common allergenic protein in two regions. Light microscopy revealed that the development of anther wall was basic type and some abnormalities were observed in microspores and pollens of Assaluyeh. Scanning electron microscopy studies showed that the apertures in considerable numbers of Assaluyeh pollens were closed. The comparison of elemental composition of pollen tectum between two regions showed that pollens of Assaluyeh have accumulated Cu on their tectum. Results obtained indicated that environmental pollution can affect protein profile, allergenic bands, structure and elemental composition of tectum of A. marina pollens.

Research paper thumbnail of Primary immunodeficiency (PP-051) PP-051-09 The immunological alteration and gene mutations in Combined ImmunodeficiencyPP-051-10 Swedish Guidelines for the assessment, diagnosis and management of 6 primary immunodeficiency states: CVID, IgG subclass deficiency, IgA deficiency, XLA, SCID and CGDP...

International Immunology Meeting Abstracts, Aug 1, 2010

In order to investigate the immunological features and characteristic of gene mutation in Chinese... more In order to investigate the immunological features and characteristic of gene mutation in Chinese patients with combined immunodeficiency, case histories were analyzed repeatedly to grasp important characteristics of the diseases. Distribution of lymphocyte subsets from peripheral blood and the level of CD40L expressing on activated CD3+ CD8− T lymphocyte surface were examined by flow cytometry. Amplify and identify exons from genes encoding CD40L and IL-2Rγc by PCR and agarose gel electrophoresis ...

Research paper thumbnail of Molecular analysis of the heavy chain variable region genes of human hybridoma clones specific for coagulation factor VIII

Thrombosis and Haemostasis, 2005

HemophiliaA is a X-linked hematologic disorder characterized by undetectable or low amounts of fu... more HemophiliaA is a X-linked hematologic disorder characterized by undetectable or low amounts of functional coagulation factor VIII (FVIII). Replacement therapy induces FVIII neutralizing antibody (Ab) (inhibitor) in a proportion of patients which makes further treatment of these patients ineffective and costly. To envisage mechanisms underlying inhibitor development, seven hybridoma clones specific for FVIII were generated from two hemophilia A patients with high titer of inhibitor. Specificity and isotype of the monoclonal antibodies (mAbs) were determined by ELISA. Immunoglobulin (Ig) variable region heavy (VH) chain gene family usage was identified by RT-PCR using VH1-6 specific primers. Nucleotide sequences of the VH gene of FVIII specific clones were determined and aligned to the most homologous germ line genes in the GenBank. Analysis of the expressed VH genes by RT-PCR revealed that the hybridomas utilized either the VH1 (71%) or the VH3 (29%) gene family. Three VH domains were encoded by V1-69 (DP-10),V1-2 (DP-8), and V1-8 (DP-15) genes and two by V1-18 (DP-14) gene, all from the VH1 gene family. Of theVH3-gene family expressing clones, one belonged to V3-66 (DP-86) and the other one to V3-21 (DP-77) germline genes. The CDR3 length was found to be highly different amongst these clones ranging from 11 to 22 amino acid residues. These data suggest that FVIII-specific Abs preferentially use VH gene segments derived from VH1 gene family. Diversity of the expressed VH genes and their CDR3 length implies that different epitopes are recognized by these mAbs.