GM Ella - Academia.edu (original) (raw)

Papers by GM Ella

Journal of Translational Medicine, 2007

Background: Melanoma tumors are known to express antigens that usually induce weak immune respons... more Background: Melanoma tumors are known to express antigens that usually induce weak immune responses of short duration. Expression of both tumor-associated antigens p53 and TRP2 by melanoma cells raises the possibility of simultaneously targeting more than one antigen in a therapeutic vaccine. In this report, we show that VacciMax ® (VM), a novel liposome-based vaccine delivery platform, can increase the immunogenicity of melanoma associated antigens, resulting in tumor elimination.

El proceso de diferenciación representa un complejo mecanismo mediante el cual las células, varia... more El proceso de diferenciación representa un complejo mecanismo mediante el cual las células, variando notablemente su morfología, adquieren una forma determinada, tamaño específico, cierta polaridad y constancia de constituyentes celulares, producto de la activación génica que permiten que dicha célula diferenciada cumpla con una función en forma óptima. Estas modificaciones pueden ser precisadas utilizando técnicas morfométricas que dan cuenta de las variaciones que caracterizan el mecanismo. Células normales de epitelio mamario de rata en cultivo, estimuladas a proliferar con el factor de crecimiento epidérmico (EGF) origina la célula HC11 GM. Ellas son inducidas a diferenciarse con inducciones hormonales de dexametasona, insulina y prolactina, generándose la célula HC11 IM. Se estudió con microscopía electrónica de transmisión estos tipos celulares con énfasis en las mitocondrias, determinando variaciones del organelo generador de energía, en lo relacionado a variables tanto cuantitativas como morfológicas, precisando así su rol en la actividad metabólica de cada tipo celular.

Cell Death and Differentiation, 2008

Two new cell surface antigens specific for the erythroid lineage were defined with cytotoxic IgM ... more Two new cell surface antigens specific for the erythroid lineage were defined with cytotoxic IgM monoclonal antibodies (McAb) (EP-1; EP-2) that were produced using BFU-E-derived colonies as immunogens. These two antigens are expressed on in vivo and in vitro derived adult and fetal erythroblasts, but not on erythrocytes. They are not detectable on resting lymphocytes, concanavalin-A (Con-A) activated lymphoblasts, granulocytes, and monocytes or granulocytic cells or macrophages present in peripheral blood or harvested from CFU-GM cultures. Cell line and tissue distributions distinguish McAb EP-1 and EP-2 from all previously described monoclonal antibodies. McAb EP-1 (for erythropoietic antigen-1) inhibits the formation of BFU-E and CFU-E, but not CFU-GM, colonies in complement-dependent cytotoxicity assays. By cell sorting analysis, about 90% of erythroid progenitors (CFU-E, BFU-E) were recovered in the antigen-positive fraction. Seven percent of the cells in this fraction were progenitors (versus 0.1% in the negative fraction). The expression of EP-1 antigen is greatly enhanced in K562 cells, using inducers of hemoglobin synthesis. McAb EP-2 fails to inhibit BFU-E and CFU-E colony formation in complement-dependent cytotoxicity assays. EP-2 antigen is predominantly expressed on in vitro derived immature erythroblasts, and it is weakly expressed on mature erythroblasts. The findings with McAb EP-1 provide evidence that erythroid progenitors (BFU-E and CFU-E) express determinants that fail to be expressed on other progenitor cells and hence appear to be unique to the erythroid lineage. McAb EP-1 and EP-2 are potentially useful for studies of erythroid differentiation and progenitor cell isolation.

Journal of Pathology, 2007

Hyperplastic Polyposis (HPPS) is a poorly characterized syndrome that increases colorectal cancer... more Hyperplastic Polyposis (HPPS) is a poorly characterized syndrome that increases colorectal cancer (CRC) risk. We aimed to provide a molecular classification of HPPS. We obtained 282 tumours from 32 putative HPPS patients with ≥ 10 hyperplastic polyps (HPs); some patients also had adenomas and CRCs. We found no good evidence of microsatellite instability (MSI) in our samples. The epithelium of HPs was monoclonal. Somatic BRAF mutations occurred in two-thirds of our patients' HPs, and KRAS2 mutations in 10%; both mutations were more common in younger cases. The respective mutation frequencies in a set of ‘sporadic’ HPs were 18% and 10%. Importantly, the putative HPPS patients generally fell into two readily defined groups, one set whose polyps had BRAF mutations, and another set whose polyps had KRAS2 mutations. The most plausible explanation for this observation is that there exist different forms of inherited predisposition to HPPS, and that these determine whether polyps follow a BRAF or KRAS2 pathway. Most adenomas and CRCs from our putative HPPS patients had ‘classical’ morphology and few of these lesions had BRAF or KRAS2 mutations. These findings suggest that tumourigenesis in HPPS does not necessarily follow the ‘serrated’ pathway. Although current definitions of HPPS are sub-optimal, we suggest that diagnosis could benefit from molecular analysis. Specifically, testing BRAF and KRAS2 mutations, and perhaps MSI, in multiple polyps could help to distinguish HPPS from sporadic HPs. We propose a specific model which would have diagnosed five more of our cases as HPPS compared with the WHO clinical criteria. Copyright © 2007 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.

Australasian Radiology, 2006

Solid and cystic papillary epithelial neoplasms (SCPEN) of the pancreas are rare tumours seen in ... more Solid and cystic papillary epithelial neoplasms (SCPEN) of the pancreas are rare tumours seen in young women. Pancreatic masses in children are extremely rare and SCPEN should be considered in the radiological differential diagnosis. Although there have been many series of SCPEN reported in the published literature in adults, only two series have focused on paediatric patients. The radiological and histopathological features of SCPEN in a paediatric patient are presented.

International Journal of Poultry Science, 2007

Two experiments were designed to estimate the effect of methionine levels (0.33 and 0.45%) with b... more Two experiments were designed to estimate the effect of methionine levels (0.33 and 0.45%) with betaine and folic acid on broiler performance. A total of 648 unsexed one week old Arbor Acres broiler chicks was randomly divided into two experiments according to dietary methionine level. Each experiment divided into nine treatment groups of 12 birds each with three replicates. The experimental diets were formulated to cover the nutrients requirements for broilers and were supplemented with betaine at 0.5, 0.75 or 1.0 gm kgG . Folic acid was added at 0.5, 0.75 or 1.0 mg kgG for each betaine level. Results can be summarized as 1 1 follows: Live body weight gain and feed conversion efficiency were significantly increased with increasing folic acid addition and increased with increasing betaine levels up to 0.75 gm kgG diet. Productive 1 performance was significantly improved by increasing different levels of betaine and folic acid. The OM, CP, EE, CF and NFE digestion coefficients were significantly (p>0.5) increased with increasing betaine or folic acid levels in the diets. The improvement of chick performance due to added betaine was depressed when chicks received diets containing recommended methionine, whereas, chicks performance improved by increasing folic acid level. Folic acid had significant effect on dressing %, the highest level received the highest dressing % recorded, while no significant effects were noticed in digestion coefficients of nutrients. Blood plasma AST and ALT decreased with increasing dietary methionine level. The highest economic efficiency was listed when diet contained the highest levels of betaine and folic acid.

Surgery, 1998

Background: The effects of α-trinositol (1D -myo-inositol-1,2,6-triphosphate, IP3) on burn-induce... more Background: The effects of α-trinositol (1D -myo-inositol-1,2,6-triphosphate, IP3) on burn-induced edema formation were investigated. Methods: Lymph flow (QL; μl/min) and lymph-to-plasma protein ratio (CL/CP) were monitored in groups of five to six dogs before and 4 hours after (1) a 5-second 100° C or 90° C foot paw scald; (2) IP3 (45 mg/kg intravenous bolus, then a 20 mg/kg/hr infusion) 30 minutes before or after 100° C scald, or 30 minutes after 90° C scald. Hind paw venous pressure was elevated and maintained by outflow restriction until reaching steady state QL and (CL/CP)min. Macromolecular reflection coefficient (1 – CL/CP) was measured. Fluid filtration coefficient (Kf; ml/min/mm Hg/100 gm) was calculated. Relative paw weight gain (%) was measured. Results: Compared with preburn values, scald uniformly produced significant increases in QL, CL/CP, and Kf. IP3 significantly (p < 0.02, ANOVA) reduced paw weight gain when given before, but not after, 100° C burn (41% ± 5% versus 18% ± 7% preburn IP3 and 31% ± 3% postburn IP3). Compared with 90° C burn animals, postburn treatment significantly (p < 0.017) attenuated 4-hour increases in QL (550 ± 87 versus 252 ± 29 μl/min), Kf (0.016 ± 00 versus 0.007 ± 00 μl/min/mm/Hg/100gm), and relative paw weight gain (28% ± 3% versus 12% ± 5%). Conclusions: α-Trinositol given after a 90° C scald blunted edema formation at the site of scald, likely through reduced transmembrane fluid flux. (Surgery 1998;123:36-45.)

Journal of Translational Medicine, 2007

Cell Death and Differentiation, 2008

Journal of Pathology, 2007

Australasian Radiology, 2006

International Journal of Poultry Science, 2007

Surgery, 1998