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Papers by GUSTAVO RAUL AGUILAR
Rev Mex Ortop Traumatol, Jun 1, 1999
Rev Mex Ortop Traumatol, Apr 1, 1999
![Research paper thumbnail of [Treatment in unstable thorax. Case report]](https://attachments.academia-assets.com/88077701/thumbnails/1.jpg)
](Acta ortopédica mexicana
We present 45 years-old male patient with bilateral luxation of all costochondral joints due to a... more We present 45 years-old male patient with bilateral luxation of all costochondral joints due to a compression mechanism while he was working beneath a car and the support was lost. He presented with floating sternum and ventilatory compromise among other injuries. Initially he was intubated and the costochondral joints were temporary fixated with dexon suture while making counter balance with 2 kg. As he was on positive pressure ventilation, lungs reexpanded we decided to place three anchors in each side of the sternum, fixated to an external aluminum support. After 10 days out of the intensive care unit, he was discharged and seen every 2 weeks. The anchors were retired in week number four and after 3 months he reintegrated to normal labor and daily activities, with limitation on flexo-extension of the left elbow by arthrogryposis.
Methods in Molecular Biology, 2008
Conventional methods to detect Salmonella spp. in foodstuffs may take up to 1 wk. Methods for pat... more Conventional methods to detect Salmonella spp. in foodstuffs may take up to 1 wk. Methods for pathogen detection are required. Real-time detection of Salmonella spp. will broaden our ability to screen large number of samples in a short time. This chapter describes a step-by-step procedure using an oligonucleotide probe that becomes fluorescent upon hybridization to the target DNA (Molecular Beacon; MB) in a real-time polymerase chain reaction (PCR) assay. The capability of the assay to detect Salmonella species from artificially inoculated fresh-and fresh-cut produce as well as ready-to-eat meats is demonstrated. The method uses internal positive and negative controls which enable researchers to detect false-negative PCR results. The procedure uses the buffered peptone water for the enrichment of Salmonella spp. and successfully detects the pathogen at low level of contamination (2-4 cells/25 g) in <24 h.
Neuroscience, 2009
Neuronal discharge and local field potential (LFP) oscillations in the olfactory bulb (OB) are mo... more Neuronal discharge and local field potential (LFP) oscillations in the olfactory bulb (OB) are modulated by odorant stimulation. The LFP oscillations have been proposed as the mechanism that facilitates synchronization of OB output neurons and the representation of similar odorants. Gamma LFP oscillations depend on the OB inhibitory network and early sensory deprivation modifies this inhibitory network. However, little is known about the LFP oscillations and neuronal discharge in the deprived OB. We examined the mitral/ tufted (MT) cells' oscillatory activity and LFP oscillations in both sensory-deprived and normal OBs in urethane anesthetized rats. We found that MT cells in deprived and normal OBs have similar basal mean firing rate; 44% of the recorded cells in deprived OB and only 8% of the cells in normal OB showed firing rate modulation by odorants, both exhibiting a similar ratio of excitatory to inhibitory responses. A fraction of MT cells exhibited oscillatory discharge centered on gamma (60-70 Hz) and beta (20 Hz) frequencies, although this feature was not consistently dependent on odorant stimulation. Odorants decreased the LFP oscillatory power in the gamma band (35-90 Hz) and increased the power in the beta band (12-30 Hz). The modulation of LFP oscillations by odorants was also predominant in the deprived (53%) compared to the normal OB (17%). In contrast, a higher fraction of MT cells' discharge was locked to the gamma LFP cycle in the normal OB. These results suggest that early unilateral olfactory deprivation increases the OB sensitivity to odorants and reduce the temporal synchrony between unitary activity and gamma LFP oscillations without altering the basal neuronal discharge.
Journal of Ethnopharmacology, 1988
An ethnobotanical survey detected 71 plants used for the treatment of vaginitis by traditional he... more An ethnobotanical survey detected 71 plants used for the treatment of vaginitis by traditional healers, health promoters and midwives. Extracts prepared by maceration of plants in 50% methanol were impregnated on absorbent paper disks and placed over plates with Candida albicans. Eight (11.3%) of these plants showed some degree of inhibition. Solanum nigrescens was chosen for further studies. A cream containing a 50% ethanolic maceration was applied daily to the vagina of female guinea pigs for 15 days and observed for another 15 days. Since no inflammatory changes were observed, this preparation was used for clinical trials. Two groups of 50 non-pregnant women with confirmed C. albicans vaginitis were treated for 15 days, one group with intra-vaginal suppositories containing S. nigrescens maceration and the other with nystatin suppositories. By statistical analysis it was demonstrated that both groups behaved in a similar beneficial way suggesting that this plant may be effective for the treatment of candidal vaginitis.
Human and Ecological Risk Assessment: An International Journal, 2012
ABSTRACT Crystalline silica and its presence in dust generated during various construction activi... more ABSTRACT Crystalline silica and its presence in dust generated during various construction activities may pose a health hazard to construction workers. In this article, the occupational exposure to crystalline silica and the related long-term health (cancer and non-cancer) risks among construction workers from five different trades (activities) were evaluated using a probabilistic approach. The predicted 95th percentile of the excess lifetime cancer risk and hazard quotients for exposure to crystalline silica ranged from 4.5× 10− 5 to 1.2× 10− 4, ...
Automation in Construction, 2013
Dermatologia Cosmetica Medica Y Quirurgica, 2012
Histoplasmosis is a worldwide systemic fungal infection, caused by a dimorphic fungus Histoplasma... more Histoplasmosis is a worldwide systemic fungal infection, caused by a dimorphic fungus Histoplasma capsulatum var. capsulatum, and in Africa, by H. duboisii. The most frequent clinical forms are pulmonary and disseminated, the latter is common in patients with AIDS, affecting cutaneous or mucous membranes with pustules, papules, molluscoid, psoriasiform or vegetating plaques, and erythema nodosum or multiform-like plaques. We report a 49 year old male patient with AIDS with a 30 day history of diarrhea, fever and disseminated papules, nodules and erythematous lesions. Diagnosis of disseminated histoplasmosis was done by serology and culture, and he was treated with amphotericin B, 1 mg/kg for 1 month, with a good response. Later on he was treated with itraconazole for several months, and after 18 months he is in complete remission.
Dermatologia Cosmetica Medica Y Quirurgica, 2010
Cyta Journal of Food, 2010
Rev Mex Ortop Traumatol, Jun 1, 1999
Rev Mex Ortop Traumatol, Apr 1, 1999
Acta ortopédica mexicana
We present 45 years-old male patient with bilateral luxation of all costochondral joints due to a... more We present 45 years-old male patient with bilateral luxation of all costochondral joints due to a compression mechanism while he was working beneath a car and the support was lost. He presented with floating sternum and ventilatory compromise among other injuries. Initially he was intubated and the costochondral joints were temporary fixated with dexon suture while making counter balance with 2 kg. As he was on positive pressure ventilation, lungs reexpanded we decided to place three anchors in each side of the sternum, fixated to an external aluminum support. After 10 days out of the intensive care unit, he was discharged and seen every 2 weeks. The anchors were retired in week number four and after 3 months he reintegrated to normal labor and daily activities, with limitation on flexo-extension of the left elbow by arthrogryposis.
Methods in Molecular Biology, 2008
Conventional methods to detect Salmonella spp. in foodstuffs may take up to 1 wk. Methods for pat... more Conventional methods to detect Salmonella spp. in foodstuffs may take up to 1 wk. Methods for pathogen detection are required. Real-time detection of Salmonella spp. will broaden our ability to screen large number of samples in a short time. This chapter describes a step-by-step procedure using an oligonucleotide probe that becomes fluorescent upon hybridization to the target DNA (Molecular Beacon; MB) in a real-time polymerase chain reaction (PCR) assay. The capability of the assay to detect Salmonella species from artificially inoculated fresh-and fresh-cut produce as well as ready-to-eat meats is demonstrated. The method uses internal positive and negative controls which enable researchers to detect false-negative PCR results. The procedure uses the buffered peptone water for the enrichment of Salmonella spp. and successfully detects the pathogen at low level of contamination (2-4 cells/25 g) in <24 h.
Neuroscience, 2009
Neuronal discharge and local field potential (LFP) oscillations in the olfactory bulb (OB) are mo... more Neuronal discharge and local field potential (LFP) oscillations in the olfactory bulb (OB) are modulated by odorant stimulation. The LFP oscillations have been proposed as the mechanism that facilitates synchronization of OB output neurons and the representation of similar odorants. Gamma LFP oscillations depend on the OB inhibitory network and early sensory deprivation modifies this inhibitory network. However, little is known about the LFP oscillations and neuronal discharge in the deprived OB. We examined the mitral/ tufted (MT) cells' oscillatory activity and LFP oscillations in both sensory-deprived and normal OBs in urethane anesthetized rats. We found that MT cells in deprived and normal OBs have similar basal mean firing rate; 44% of the recorded cells in deprived OB and only 8% of the cells in normal OB showed firing rate modulation by odorants, both exhibiting a similar ratio of excitatory to inhibitory responses. A fraction of MT cells exhibited oscillatory discharge centered on gamma (60-70 Hz) and beta (20 Hz) frequencies, although this feature was not consistently dependent on odorant stimulation. Odorants decreased the LFP oscillatory power in the gamma band (35-90 Hz) and increased the power in the beta band (12-30 Hz). The modulation of LFP oscillations by odorants was also predominant in the deprived (53%) compared to the normal OB (17%). In contrast, a higher fraction of MT cells' discharge was locked to the gamma LFP cycle in the normal OB. These results suggest that early unilateral olfactory deprivation increases the OB sensitivity to odorants and reduce the temporal synchrony between unitary activity and gamma LFP oscillations without altering the basal neuronal discharge.
Journal of Ethnopharmacology, 1988
An ethnobotanical survey detected 71 plants used for the treatment of vaginitis by traditional he... more An ethnobotanical survey detected 71 plants used for the treatment of vaginitis by traditional healers, health promoters and midwives. Extracts prepared by maceration of plants in 50% methanol were impregnated on absorbent paper disks and placed over plates with Candida albicans. Eight (11.3%) of these plants showed some degree of inhibition. Solanum nigrescens was chosen for further studies. A cream containing a 50% ethanolic maceration was applied daily to the vagina of female guinea pigs for 15 days and observed for another 15 days. Since no inflammatory changes were observed, this preparation was used for clinical trials. Two groups of 50 non-pregnant women with confirmed C. albicans vaginitis were treated for 15 days, one group with intra-vaginal suppositories containing S. nigrescens maceration and the other with nystatin suppositories. By statistical analysis it was demonstrated that both groups behaved in a similar beneficial way suggesting that this plant may be effective for the treatment of candidal vaginitis.
Human and Ecological Risk Assessment: An International Journal, 2012
ABSTRACT Crystalline silica and its presence in dust generated during various construction activi... more ABSTRACT Crystalline silica and its presence in dust generated during various construction activities may pose a health hazard to construction workers. In this article, the occupational exposure to crystalline silica and the related long-term health (cancer and non-cancer) risks among construction workers from five different trades (activities) were evaluated using a probabilistic approach. The predicted 95th percentile of the excess lifetime cancer risk and hazard quotients for exposure to crystalline silica ranged from 4.5× 10− 5 to 1.2× 10− 4, ...
Automation in Construction, 2013
Dermatologia Cosmetica Medica Y Quirurgica, 2012
Histoplasmosis is a worldwide systemic fungal infection, caused by a dimorphic fungus Histoplasma... more Histoplasmosis is a worldwide systemic fungal infection, caused by a dimorphic fungus Histoplasma capsulatum var. capsulatum, and in Africa, by H. duboisii. The most frequent clinical forms are pulmonary and disseminated, the latter is common in patients with AIDS, affecting cutaneous or mucous membranes with pustules, papules, molluscoid, psoriasiform or vegetating plaques, and erythema nodosum or multiform-like plaques. We report a 49 year old male patient with AIDS with a 30 day history of diarrhea, fever and disseminated papules, nodules and erythematous lesions. Diagnosis of disseminated histoplasmosis was done by serology and culture, and he was treated with amphotericin B, 1 mg/kg for 1 month, with a good response. Later on he was treated with itraconazole for several months, and after 18 months he is in complete remission.
Dermatologia Cosmetica Medica Y Quirurgica, 2010
Cyta Journal of Food, 2010