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Papers by Asha Gangadharan
Materials Today: Proceedings, 2020
Bioprospecting is the searching of novel bioactive natural products from unexplored natural envir... more Bioprospecting is the searching of novel bioactive natural products from unexplored natural environment. The aim of the study was to isolate novel therapeutic agents from marine bacterium. The marine bacterium was identified as Serratia marcescens using molecular identification methods. The secondary metabolites were extracted and showed single spot on TLC at Rf of 0.74. The metabolite showed good antioxidant and antibacterial activity against Staphylococcus aureus, pseudomonas sp, E. coli, Klebsiella sp, Salmonella paratyphi A. The antielastase activity was appreciable when compared to standard elastase inhibitor. The structural characterization of purified metabolite by GC-MS revealed 2,4-ditert butylphenol as major component.
Materials Today: Proceedings, 2020
Hemigraphis colorata is an indigenous wound healing plant used by various tribes in India since t... more Hemigraphis colorata is an indigenous wound healing plant used by various tribes in India since time immortal. This study was aimed to evaluate antibacterial, antioxidant and antielastase effect of hexane extract of Hemigraphis colorata and to identify its bioactive principles. The antioxidant and antibacterial activity of hexane extract were analyzed using in-vitro DPPH scavenging assay and Agar well diffusion method respectively. The antielastase assay was conducted using porcine pancreatic elastase. Biological activity studies revealed that hexane extract of Hemigraphis colorata possess significant antibacterial, antioxidant and antielastase activity. The major phytochemical compound was found to be squalene by GC-MS analysis.
Indian Journal of Natural Products and Resources
Leucas aspera (Willd.) Link has been used for curing various ailments including skin disease in t... more Leucas aspera (Willd.) Link has been used for curing various ailments including skin disease in traditional medicine for decades. The aim of the study was to evaluate the in vitro antioxidant potential and anti-elastase activity of L. aspera (Willd.) Link. The hexane, ethyl acetate, acetone and ethanol extracts were prepared using the Soxhlet extraction method. The phytochemical analysis, in vitro free radical scavenging, and anti-elastase activities, were conducted. The total phenol and flavonoids compounds were found to be significantly high for the hexane extract and the ethyl acetate extract respectively. In vitro antioxidant assays like percentage reducing power, DPPH radical scavenging activity, the total antioxidant capacity by FRAP assay, revealed that the acetone extract of the plant L. aspera possesses significantly the highest activity. The anti elastase assay revealed that all the extracts possess elastase inhibitory activity and the hexane extract possessed significantly highest activity with IC 50 of 247.42 µg/mL, at a significant level (α) <0.05. The present result supports the traditional use of L. aspera.
The systematic evaluation of the anti-inflammatory activity of indigenous plants used in wound he... more The systematic evaluation of the anti-inflammatory activity of indigenous plants used in wound healing will facilitate the development of efficient wound healing drugs. The present study was undertaken to evaluate the efficiency of anti-inflammatory activity of three plants used by traditional healers, viz:Hemigraphis colorata (Blume) H.G. Hallier, Leucas aspera (Willd) Link and Biophytum sensitivum (L.) DC. Hexane, ethyl acetate, acetone and ethanol extracts of the plants, were subjected to evaluate Human RBC membrane stabilization potential, which influence the process of inflammation. Among the extracts screened the hexane and acetone extract of Leucas aspera, acetone extract of Biophytum sensitivum and ethanol extract of Hemigraphis colorata were exhibited significantly better activity than the other extracts and standard antiinflammatory drug Diclofenac sodium. These observations will stimulate further research in the clinical application of the plants under study. The study pr...
Plants have always played a major role in the treatment of human diseases. Worldwide interest in ... more Plants have always played a major role in the treatment of human diseases. Worldwide interest in the use of medicinal plants is increasing nowadays. Hemigraphis coloratabelonging to the family Acanthaceae, was used for the treatment of various diseases in traditional system of medicine. In this study phytochemical analysis of Hemigraphis colorata, its anti oxidant effect and anti elastase activity were evaluated. The antioxidant effec t of alcoholic and aqueous extracts of Hemigraphis colorata was studied by DPPH radical scavenging assay and by reducing power. The primary phytochemical screening showed the presence of carbohydrates, proteins, alkaloids, phenolic compounds, flavonoids, oils, steroids and terpe noids in alcoholic extract and the presence of alkaloids, flavonoids, and phenolic compounds in aqueous extracts. The quantitative phytochemical analysis revealed that the total phenol, flavanoids and flavanols contents were 315, 13.68 and 6.27 mg and 440,7.43 and 6.27 mg respe...
The proper awareness and right attitude towards the protection of natural resources is of great c... more The proper awareness and right attitude towards the protection of natural resources is of great concern in the modern world. The major concern among environmental pollution is plastic pollution. Plastic serves plethora of uses in our society due to its durability, but the toxicity caused by plastics in the environment is inevitable as it is non-biodegradable. The purpose of environmental education is to acquaint and sensitize college students to the environmental problems and concern, in order to instil upon them, the apt social attitude and healthy approach towards environmental protection. Therefore, it is required to measure their level of awareness and attitude towards environmental concerns. This study was aimed to investigate the environmental awareness among graduate students and to evaluate the attitude and behaviours related to environmental concerns. The survey had 22 questions that tested their knowledge, awareness, behaviour and attitude of college students on environmen...
INTERNATIONAL CONFERENCE ON SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS: STAM 20, 2020
Squalene synthase is a key enzyme of isoprenoid biosynthetic pathway, which is capable of diverti... more Squalene synthase is a key enzyme of isoprenoid biosynthetic pathway, which is capable of diverting carbon flow specifically to the biosynthesis of sterol and hence, it is considered as a potential regulatory point for sterol metabolism. It is also a key regulatory enzyme in cholesterol biosynthesis pathway. Squalene synthase, one of the primary enzymes produced in plants which helps in the biosynthetic pathway of terpenoid and other steroid compounds. In addition to this, variants of squalene synthase gene appear to regulate plasma cholesterol levels in human population and therefore it can be linked to cardiovascular diseases. Squalene synthase inhibitors are effective in lowering plasma levels of total cholesterol. In Artemisia annua L. plants, the carbon needed for both sterol and artemisinin biosynthetic pathway is provided by mevalonate pathway in the form of farnesyl pyrophosphate (FPP). Hence, squalene synthase is a rate limiting enzyme of sterol biosynthetic pathway which compete with amospha-4,11-dienesynthase, which is the rate limiting enzyme of artemisinin biosynthetic pathway for the FPP. This competition will deplete the FPP pool towards artemisinin biosynthesis. So, squalene synthase, is an important target for the down regulation of sterols.
Materials Today: Proceedings, 2020
Bioprospecting is the searching of novel bioactive natural products from unexplored natural envir... more Bioprospecting is the searching of novel bioactive natural products from unexplored natural environment. The aim of the study was to isolate novel therapeutic agents from marine bacterium. The marine bacterium was identified as Serratia marcescens using molecular identification methods. The secondary metabolites were extracted and showed single spot on TLC at Rf of 0.74. The metabolite showed good antioxidant and antibacterial activity against Staphylococcus aureus, pseudomonas sp, E. coli, Klebsiella sp, Salmonella paratyphi A. The antielastase activity was appreciable when compared to standard elastase inhibitor. The structural characterization of purified metabolite by GC-MS revealed 2,4-ditert butylphenol as major component.
Materials Today: Proceedings, 2020
Bioprospecting is the searching of novel bioactive natural products from unexplored natural envir... more Bioprospecting is the searching of novel bioactive natural products from unexplored natural environment. The aim of the study was to isolate novel therapeutic agents from marine bacterium. The marine bacterium was identified as Serratia marcescens using molecular identification methods. The secondary metabolites were extracted and showed single spot on TLC at Rf of 0.74. The metabolite showed good antioxidant and antibacterial activity against Staphylococcus aureus, pseudomonas sp, E. coli, Klebsiella sp, Salmonella paratyphi A. The antielastase activity was appreciable when compared to standard elastase inhibitor. The structural characterization of purified metabolite by GC-MS revealed 2,4-ditert butylphenol as major component.
Materials Today: Proceedings, 2020
Hemigraphis colorata is an indigenous wound healing plant used by various tribes in India since t... more Hemigraphis colorata is an indigenous wound healing plant used by various tribes in India since time immortal. This study was aimed to evaluate antibacterial, antioxidant and antielastase effect of hexane extract of Hemigraphis colorata and to identify its bioactive principles. The antioxidant and antibacterial activity of hexane extract were analyzed using in-vitro DPPH scavenging assay and Agar well diffusion method respectively. The antielastase assay was conducted using porcine pancreatic elastase. Biological activity studies revealed that hexane extract of Hemigraphis colorata possess significant antibacterial, antioxidant and antielastase activity. The major phytochemical compound was found to be squalene by GC-MS analysis.
Indian Journal of Natural Products and Resources
Leucas aspera (Willd.) Link has been used for curing various ailments including skin disease in t... more Leucas aspera (Willd.) Link has been used for curing various ailments including skin disease in traditional medicine for decades. The aim of the study was to evaluate the in vitro antioxidant potential and anti-elastase activity of L. aspera (Willd.) Link. The hexane, ethyl acetate, acetone and ethanol extracts were prepared using the Soxhlet extraction method. The phytochemical analysis, in vitro free radical scavenging, and anti-elastase activities, were conducted. The total phenol and flavonoids compounds were found to be significantly high for the hexane extract and the ethyl acetate extract respectively. In vitro antioxidant assays like percentage reducing power, DPPH radical scavenging activity, the total antioxidant capacity by FRAP assay, revealed that the acetone extract of the plant L. aspera possesses significantly the highest activity. The anti elastase assay revealed that all the extracts possess elastase inhibitory activity and the hexane extract possessed significantly highest activity with IC 50 of 247.42 µg/mL, at a significant level (α) <0.05. The present result supports the traditional use of L. aspera.
The systematic evaluation of the anti-inflammatory activity of indigenous plants used in wound he... more The systematic evaluation of the anti-inflammatory activity of indigenous plants used in wound healing will facilitate the development of efficient wound healing drugs. The present study was undertaken to evaluate the efficiency of anti-inflammatory activity of three plants used by traditional healers, viz:Hemigraphis colorata (Blume) H.G. Hallier, Leucas aspera (Willd) Link and Biophytum sensitivum (L.) DC. Hexane, ethyl acetate, acetone and ethanol extracts of the plants, were subjected to evaluate Human RBC membrane stabilization potential, which influence the process of inflammation. Among the extracts screened the hexane and acetone extract of Leucas aspera, acetone extract of Biophytum sensitivum and ethanol extract of Hemigraphis colorata were exhibited significantly better activity than the other extracts and standard antiinflammatory drug Diclofenac sodium. These observations will stimulate further research in the clinical application of the plants under study. The study pr...
Plants have always played a major role in the treatment of human diseases. Worldwide interest in ... more Plants have always played a major role in the treatment of human diseases. Worldwide interest in the use of medicinal plants is increasing nowadays. Hemigraphis coloratabelonging to the family Acanthaceae, was used for the treatment of various diseases in traditional system of medicine. In this study phytochemical analysis of Hemigraphis colorata, its anti oxidant effect and anti elastase activity were evaluated. The antioxidant effec t of alcoholic and aqueous extracts of Hemigraphis colorata was studied by DPPH radical scavenging assay and by reducing power. The primary phytochemical screening showed the presence of carbohydrates, proteins, alkaloids, phenolic compounds, flavonoids, oils, steroids and terpe noids in alcoholic extract and the presence of alkaloids, flavonoids, and phenolic compounds in aqueous extracts. The quantitative phytochemical analysis revealed that the total phenol, flavanoids and flavanols contents were 315, 13.68 and 6.27 mg and 440,7.43 and 6.27 mg respe...
The proper awareness and right attitude towards the protection of natural resources is of great c... more The proper awareness and right attitude towards the protection of natural resources is of great concern in the modern world. The major concern among environmental pollution is plastic pollution. Plastic serves plethora of uses in our society due to its durability, but the toxicity caused by plastics in the environment is inevitable as it is non-biodegradable. The purpose of environmental education is to acquaint and sensitize college students to the environmental problems and concern, in order to instil upon them, the apt social attitude and healthy approach towards environmental protection. Therefore, it is required to measure their level of awareness and attitude towards environmental concerns. This study was aimed to investigate the environmental awareness among graduate students and to evaluate the attitude and behaviours related to environmental concerns. The survey had 22 questions that tested their knowledge, awareness, behaviour and attitude of college students on environmen...
INTERNATIONAL CONFERENCE ON SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS: STAM 20, 2020
Squalene synthase is a key enzyme of isoprenoid biosynthetic pathway, which is capable of diverti... more Squalene synthase is a key enzyme of isoprenoid biosynthetic pathway, which is capable of diverting carbon flow specifically to the biosynthesis of sterol and hence, it is considered as a potential regulatory point for sterol metabolism. It is also a key regulatory enzyme in cholesterol biosynthesis pathway. Squalene synthase, one of the primary enzymes produced in plants which helps in the biosynthetic pathway of terpenoid and other steroid compounds. In addition to this, variants of squalene synthase gene appear to regulate plasma cholesterol levels in human population and therefore it can be linked to cardiovascular diseases. Squalene synthase inhibitors are effective in lowering plasma levels of total cholesterol. In Artemisia annua L. plants, the carbon needed for both sterol and artemisinin biosynthetic pathway is provided by mevalonate pathway in the form of farnesyl pyrophosphate (FPP). Hence, squalene synthase is a rate limiting enzyme of sterol biosynthetic pathway which compete with amospha-4,11-dienesynthase, which is the rate limiting enzyme of artemisinin biosynthetic pathway for the FPP. This competition will deplete the FPP pool towards artemisinin biosynthesis. So, squalene synthase, is an important target for the down regulation of sterols.
Materials Today: Proceedings, 2020
Bioprospecting is the searching of novel bioactive natural products from unexplored natural envir... more Bioprospecting is the searching of novel bioactive natural products from unexplored natural environment. The aim of the study was to isolate novel therapeutic agents from marine bacterium. The marine bacterium was identified as Serratia marcescens using molecular identification methods. The secondary metabolites were extracted and showed single spot on TLC at Rf of 0.74. The metabolite showed good antioxidant and antibacterial activity against Staphylococcus aureus, pseudomonas sp, E. coli, Klebsiella sp, Salmonella paratyphi A. The antielastase activity was appreciable when compared to standard elastase inhibitor. The structural characterization of purified metabolite by GC-MS revealed 2,4-ditert butylphenol as major component.