Gefu Wang-Pruski - Academia.edu (original) (raw)

Papers by Gefu Wang-Pruski

Research paper thumbnail of Hybrid potato breeding for improved varieties

Research paper thumbnail of Use of quantitative proteomics to study induced resistance of plants to pathogens

Journal of Proteomics & Bioinformatics, Aug 20, 2013

Research paper thumbnail of Visualization of molecular and cellular events with green fluorescent proteins in developing embryos: a review

Luminescence, 2003

An Erratum has been published for this article in Luminescence (2003) 18(4) 243 During the past 5... more An Erratum has been published for this article in Luminescence (2003) 18(4) 243 During the past 5 years, green fluorescent protein (GFP) has become one of the most widely used in vivo protein markers for studying a number of different molecular processes during development, such as promoter activation, gene expression, protein trafficking and cell lineage determination. GFP fluorescence allows observation of dynamic developmental processes in real time, in both transiently and stably transformed cells, as well as in live embryos. In this review, we include the most up‐to‐date use of GFP during embryonic development and point out the unique contribution of GFP visualization, which resulted in novel discoveries. Copyright © 2002 John Wiley & Sons, Ltd.

Research paper thumbnail of Digital-imaging analysis - A new method for evaluation of potato after-cooking darkening ; ACTA HORTICULTURAE

After-cooking darkening (ACD) phenomenon occurs when boiled or steamed potato tubers become grayi... more After-cooking darkening (ACD) phenomenon occurs when boiled or steamed potato tubers become grayish dark on exposure to air. The degree of darkening is controlled genetically and is strongly influenced by environmental factors. The pigment responsible for ACD is a complex of chlorogenic acid and iron, which is formed during cooking and oxidizes during cooling to a colored ferric di-chlorogenic acid complex. The evaluation of ACD is thus based either on the amount of chlorogenic acid in tubers (destructive method) or on the determination of the degree of color intensity on cut tuber surfaces (non-destructive method). The destructive method is HPLC based, complicated, and time consuming, particularly when a large number of samples is being evaluated. Thus, despite its deficiency, non-destructive visual examination is routinely utilized in many breeding programs. The objective of this study was to determine whether digital imaging analysis can be adapted to the evaluation of ACD. The method is based on the direct capture of the cooked and cut tuber surface image using a cooled CCD camera attached to a digital imaging system. The degree of the dark color is then measured by pixels using an imaging acquisition software. The system is calibrated at 0-255 pixel levels (0 as black, 255 as white) as standard, therefore the read values directly reflect the degree of darkening. The measurement procedure is fast, reliable, simple, and particularly applicable for handling a large number of samples. This is the first report on evaluation of ACD using a digital imaging system.

Research paper thumbnail of Evaluation of Reference Genes Suitable for Gene Expression during Root Enlargement in Cherry Radish Based on Transcriptomic Data

Horticulturae, Dec 22, 2022

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Research paper thumbnail of Estimation of soil nitrogen supply in potato fields using a plant bioassay approach

Canadian Journal of Soil Science, Aug 1, 2005

Soil N supply is an important contributor of N to crop production; however, there is a lack of pr... more Soil N supply is an important contributor of N to crop production; however, there is a lack of practical methods for routine estimation of soil N supply under field conditions. This study evaluated sampling just prior to topkill of whole potato plants that received no fertilizer N as a field bioassay of soil N supply. Three experiments were performed. In exp. 1, field trials were conducted to test if P and K fertilization, with no N fertilization, influenced plant biomass and N accumulation at topkill. In exp. 2, plant N accumulation at topkill in unfertilized plots was compared with mineral N accumulation in vegetation-free plots. In exp. 3, estimates of soil N supply were obtained from 56 sites from 1999 to 2003 using a survey approach where plant N accumulation at topkill, and soil mineral N content to 30-cm depth at planting and at tuber harvest were measured. Application of P and K fertilizer had no significant effect on plant N accumulation in two trials, and resulted in a small increase in plant N accumulation in a third trial. Zero fertilizer plots, which can be more readily established in commercial potato fields, can therefore be used instead of zero fertilizer N plots to estimate soil N supply. In exp. 2, estimates of soil N supply were generally comparable between plant N accumulation at topkill and maximum soil NO 3-N accumulation in vegetation-free plots; therefore, the plant bioassay approach is a valid means of estimation of plant available soil N supply. Plant N accumulation at topkill in exp. 3 averaged 86 kg N ha-1 , and ranged from 26 to 162 kg N ha-1. Plant N accumulation was higher for sites with a preceding forage crop compared with a preceding cereal or potato crop. Plant N accumulation was generally higher in years with warmer growing season temperatures. Soil NO 3-N content at harvest in exp. 3 was less than 20 kg N ha-1 , indicating that residual soil mineral N content was low at the time of plant N accumulation measurement. Soil NO 3-N content at planting was generally small relative to plant N accumulation, indicating that soil N supply in this region is controlled primarily by growing season soil N mineralization. Use of a plant bioassay approach provides a practical means to quantify climate, soil and management effects on plant available soil N supply in potato production.

Research paper thumbnail of Physiological analysis and transcriptome profiling reveals the impact of microplastic on melon (Cucumis melo L.) seed germination and seedling growth

Journal of Plant Physiology, Aug 1, 2023

Research paper thumbnail of The function of BoTCP25 in the regulation of leaf development of Chinese kale

Frontiers in Plant Science, Apr 18, 2023

Research paper thumbnail of Physiological and transcriptomic responses of water spinach (Ipomoea aquatica) to prolonged heat stress

BMC Genomics, Aug 3, 2020

Research paper thumbnail of Genome-wide identification, characterization, and expression analysis related to autotoxicity of the GST gene family in Cucumis melo L

Plant Physiology and Biochemistry, Oct 1, 2020

Glutathione S-transferase (GST) plays an important role in plant resistance to biotic and abiotic... more Glutathione S-transferase (GST) plays an important role in plant resistance to biotic and abiotic stresses. In this paper, the characteristics of melon GST gene family members were analyzed from a genome-wide perspective. Forty-nine GSTs were identified in melon genome, belonging to eight classes. Through the phylogenetic analysis of GST proteins in melon and other plants, it was found that members from the same subfamily in different species clustered together, indicating that the subfamilies of GST have diversified before the divergence within these species. The results of chromosome mapping showed that GSTs were present in all chromosomes except for chromosome 5. Gene replication events played an important role in the expansion and evolution of melon GST gene family. Ten GSTs with significant differential expression were screened in the transcriptome database related to melon autotoxicity stress. The differential expression of these 10 GSTs was detected in roots and leaves of melon seedlings treated with cinnamic acid. The relative expression level of CmGSTU7, CmGSTU10, CmGSTU18, CmGSTF2 and CmGSTL1 in roots of melon seedlings was significantly higher than that in control group. It suggested that the five GSTs might play an important role in cinnamic acid mediated autotoxicity stress in melon. The results of this paper were helpful to reveal the evolution and functional succession of GST family and further understand the response of GST to autotoxicity stress in melon.

Research paper thumbnail of Comparative transcriptome analyses revealed different heat stress responses in high- and low-GS Brassica alboglabra sprouts

BMC Genomics, Apr 4, 2019

Background: Chinese kale (Brassica alboglabra) contains high nutritional elements and functional ... more Background: Chinese kale (Brassica alboglabra) contains high nutritional elements and functional molecules, especially anticarcinogenic and antioxidant glucosinolates (GS), which was highly affected by environment temperature. To investigate the link of GS biosynthesis with heat stress response in Chinese kale, global transcription profiles of high-GS line (HG), low-GS line (LG), high-GS line under heat stress (HGT) and low-GS line under heat stress (LGT) were analyzed. Results: Based on three biological replicates of each RNA sequencing data, 3901, 4062 and 2396 differentially expressed genes in HG vs HGT, LG vs LGT and HGT vs LGT were obtained, respectively. GO annotation, KEGG pathway analysis and a comprehensive analysis of DEGs showed a strong correlation between the GS biosynthesis and heat stress response. It was noticed that 11 differentially expressed genes tied to the GS biosynthesis were down-regulated, 23 heat shock transcription factors and 61 heat shock proteins were up-regulated upon the heat treatment. Another two Chinese kale varieties Cuibao and Shunbao with high-and low-GS content respectively, were used to validate the relationship of GS content and heat-response, and the results showed that high-GS content variety were more thermotolerant than the low-GS content one although GS significantly decreased in both varieties under heat stress. In addition, HSP100/ClpB, HSP90, HSP70 and sHSPs were differentially expressed in high-and low-GS varieties. Notably, HSP90 and sHSPs showed an obviously early response to heat stress than other related genes. Conclusion: The higher heat resistance of high-GS Chinese kale and the sharp decrease of glucosinolate content under heat stress indicated a strong relationship of GS accumulation and heat stress response. Combined with the previous report on the low expression of HSP90 at elevated temperatures in GS-deficient mutant TU8 of Arabidopsis, the differential expression pattern of HSP90 in high-and low-GS varieties and its early heat response implied it might be a key regulator in GS metabolism and heat-resistance in Chinese kale.

Research paper thumbnail of Digital imaging for the evaluation of potato after-cooking darkening: correcting the effect of flesh colour

International Journal of Food Science and Technology, Dec 1, 2009

Research paper thumbnail of Transcriptome profiling of periwinkle infected with Huanglongbing (‘Candidatus Liberibacter asiaticus’)

European Journal of Plant Pathology, Oct 26, 2018

Research paper thumbnail of Chemical triggered defense mechanisms against Phytophthora infestans

Advances in Crop Science and Technology, Jul 21, 2016

L ate blight is the most devastating disease in potato production around the world. It is caused ... more L ate blight is the most devastating disease in potato production around the world. It is caused by Phytophthora infestans which mutates rapidly. Renewed control strategies aimed at higher efficiency and lower environmental impact are urgently required. Research carried out for a decade in our laboratories demonstrated that we can prime potato plants to boost diverse defense functions against Phytophthora infestans. Priming could reduce the use of fungicides and pesticides and ensure the protection of human health and the environment. In this talk, I will present our knowledge about the molecular mechanisms of priming against P. infestans based on our studies using proteomics, metabolomics and gene expression profiling tools. Biography Gefu Wang-Pruski has received her PhD from University of Alberta in Canada and completed her Post doctorate from Loma Linda University in Southern California. She has over 30 year's research experience. She is currently a full Professor at Dalhousie University. She has been leading over 20 national and international research programs in the past 15 years and generated over 50 peer-reviewed publications. She has been serving as an Editorial Board Member for many journals.

Research paper thumbnail of Digital Imaging Analysis - a New Method for Evaluation of Potato After-Cooking Darkening

Research paper thumbnail of Visualization of Intracellular Bacterial Infection in Tumor Cell Lines

Bioluminescence and Chemiluminescence, 2002

Research paper thumbnail of Phosphite uptake and distribution in potato tubers following foliar and postharvest applications of phosphite-based fungicides for late blight control

Annals of Applied Biology, 2016

Research paper thumbnail of Integration of Small RNA and Transcriptome Sequencing Reveal the Roles of miR395 and ATP Sulfurylase in Developing Seeds of Chinese Kale

Frontiers in Plant Science

Seed development is closely related to plant production and reproduction, and MicroRNAs (miRNA) i... more Seed development is closely related to plant production and reproduction, and MicroRNAs (miRNA) is widely involved in plant development including seed development. Chinese kale, as a Brassicaceae vegetable, mainly depends on seed for proper reproduction. In the present study, Chinese kale seed and silique at different stages were selected to establish small RNA (sRNA) libraries including silique wall sRNA libraries at torpedo-embryo stage (PC), silique wall sRNA libraries at cotyledonary-embryo stage (PD), seed sRNA libraries at torpedo-embryo stage (SC), and seed sRNA libraries at cotyledonary-embryo stage (SD). The results showed that miRNA expressed differentially in the seeds and corresponding siliques at different stages. To further clarify the functional mode of miRNA in the process of seed development, Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis was performed on target genes of the differentially expressed miRNAs, and these target genes were mainly enri...

Research paper thumbnail of Irrigation Effect on Yield, Skin Blemishes, Phellem Formation, and Total Phenolics of Red Potatoes

Plants

Dark Red Norland is an important potato cultivar in the fresh market due to its attractive bright... more Dark Red Norland is an important potato cultivar in the fresh market due to its attractive bright, red colour, and good yield. However, skin blemishes such as silver patch, surface cracking, and russeting can negatively influence the tuber skin quality and marketability. It is well known that potato is a drought-sensitive plant. This study was conducted to determine whether irrigation would affect Dark Red Norland’s yield and skin quality. A three-year field trial was conducted by Peak of the Market in Manitoba, Canada. Plants were treated under both irrigation and rainfed conditions. The results show that irrigation increased the total yield by 20.6% and reduced the severity of surface cracking by 48.5%. Microscopy imaging analysis demonstrated that tubers from the rainfed trials formed higher numbers of suberized cell layers than those of the irrigated potatoes, with a difference of 0.360 to 0.652 layers in normal skins. Surface cracking and silver patch skins had more suberized c...

Research paper thumbnail of Aluminum in plant: Benefits, toxicity and tolerance mechanisms

Frontiers in Plant Science

Aluminum (Al) is the third most ubiquitous metal in the earth’s crust. A decrease in soil pH belo... more Aluminum (Al) is the third most ubiquitous metal in the earth’s crust. A decrease in soil pH below 5 increases its solubility and availability. However, its impact on plants depends largely on concentration, exposure time, plant species, developmental age, and growing conditions. Although Al can be beneficial to plants by stimulating growth and mitigating biotic and abiotic stresses, it remains unknown how Al mediates these effects since its biological significance in cellular systems is still unidentified. Al is considered a major limiting factor restricting plant growth and productivity in acidic soils. It instigates a series of phytotoxic symptoms in several Al-sensitive crops with inhibition of root growth and restriction of water and nutrient uptake as the obvious symptoms. This review explores advances in Al benefits, toxicity and tolerance mechanisms employed by plants on acidic soils. These insights will provide directions and future prospects for potential crop improvement.

Research paper thumbnail of Hybrid potato breeding for improved varieties

Research paper thumbnail of Use of quantitative proteomics to study induced resistance of plants to pathogens

Journal of Proteomics & Bioinformatics, Aug 20, 2013

Research paper thumbnail of Visualization of molecular and cellular events with green fluorescent proteins in developing embryos: a review

Luminescence, 2003

An Erratum has been published for this article in Luminescence (2003) 18(4) 243 During the past 5... more An Erratum has been published for this article in Luminescence (2003) 18(4) 243 During the past 5 years, green fluorescent protein (GFP) has become one of the most widely used in vivo protein markers for studying a number of different molecular processes during development, such as promoter activation, gene expression, protein trafficking and cell lineage determination. GFP fluorescence allows observation of dynamic developmental processes in real time, in both transiently and stably transformed cells, as well as in live embryos. In this review, we include the most up‐to‐date use of GFP during embryonic development and point out the unique contribution of GFP visualization, which resulted in novel discoveries. Copyright © 2002 John Wiley & Sons, Ltd.

Research paper thumbnail of Digital-imaging analysis - A new method for evaluation of potato after-cooking darkening ; ACTA HORTICULTURAE

After-cooking darkening (ACD) phenomenon occurs when boiled or steamed potato tubers become grayi... more After-cooking darkening (ACD) phenomenon occurs when boiled or steamed potato tubers become grayish dark on exposure to air. The degree of darkening is controlled genetically and is strongly influenced by environmental factors. The pigment responsible for ACD is a complex of chlorogenic acid and iron, which is formed during cooking and oxidizes during cooling to a colored ferric di-chlorogenic acid complex. The evaluation of ACD is thus based either on the amount of chlorogenic acid in tubers (destructive method) or on the determination of the degree of color intensity on cut tuber surfaces (non-destructive method). The destructive method is HPLC based, complicated, and time consuming, particularly when a large number of samples is being evaluated. Thus, despite its deficiency, non-destructive visual examination is routinely utilized in many breeding programs. The objective of this study was to determine whether digital imaging analysis can be adapted to the evaluation of ACD. The method is based on the direct capture of the cooked and cut tuber surface image using a cooled CCD camera attached to a digital imaging system. The degree of the dark color is then measured by pixels using an imaging acquisition software. The system is calibrated at 0-255 pixel levels (0 as black, 255 as white) as standard, therefore the read values directly reflect the degree of darkening. The measurement procedure is fast, reliable, simple, and particularly applicable for handling a large number of samples. This is the first report on evaluation of ACD using a digital imaging system.

Research paper thumbnail of Evaluation of Reference Genes Suitable for Gene Expression during Root Enlargement in Cherry Radish Based on Transcriptomic Data

Horticulturae, Dec 22, 2022

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Research paper thumbnail of Estimation of soil nitrogen supply in potato fields using a plant bioassay approach

Canadian Journal of Soil Science, Aug 1, 2005

Soil N supply is an important contributor of N to crop production; however, there is a lack of pr... more Soil N supply is an important contributor of N to crop production; however, there is a lack of practical methods for routine estimation of soil N supply under field conditions. This study evaluated sampling just prior to topkill of whole potato plants that received no fertilizer N as a field bioassay of soil N supply. Three experiments were performed. In exp. 1, field trials were conducted to test if P and K fertilization, with no N fertilization, influenced plant biomass and N accumulation at topkill. In exp. 2, plant N accumulation at topkill in unfertilized plots was compared with mineral N accumulation in vegetation-free plots. In exp. 3, estimates of soil N supply were obtained from 56 sites from 1999 to 2003 using a survey approach where plant N accumulation at topkill, and soil mineral N content to 30-cm depth at planting and at tuber harvest were measured. Application of P and K fertilizer had no significant effect on plant N accumulation in two trials, and resulted in a small increase in plant N accumulation in a third trial. Zero fertilizer plots, which can be more readily established in commercial potato fields, can therefore be used instead of zero fertilizer N plots to estimate soil N supply. In exp. 2, estimates of soil N supply were generally comparable between plant N accumulation at topkill and maximum soil NO 3-N accumulation in vegetation-free plots; therefore, the plant bioassay approach is a valid means of estimation of plant available soil N supply. Plant N accumulation at topkill in exp. 3 averaged 86 kg N ha-1 , and ranged from 26 to 162 kg N ha-1. Plant N accumulation was higher for sites with a preceding forage crop compared with a preceding cereal or potato crop. Plant N accumulation was generally higher in years with warmer growing season temperatures. Soil NO 3-N content at harvest in exp. 3 was less than 20 kg N ha-1 , indicating that residual soil mineral N content was low at the time of plant N accumulation measurement. Soil NO 3-N content at planting was generally small relative to plant N accumulation, indicating that soil N supply in this region is controlled primarily by growing season soil N mineralization. Use of a plant bioassay approach provides a practical means to quantify climate, soil and management effects on plant available soil N supply in potato production.

Research paper thumbnail of Physiological analysis and transcriptome profiling reveals the impact of microplastic on melon (Cucumis melo L.) seed germination and seedling growth

Journal of Plant Physiology, Aug 1, 2023

Research paper thumbnail of The function of BoTCP25 in the regulation of leaf development of Chinese kale

Frontiers in Plant Science, Apr 18, 2023

Research paper thumbnail of Physiological and transcriptomic responses of water spinach (Ipomoea aquatica) to prolonged heat stress

BMC Genomics, Aug 3, 2020

Research paper thumbnail of Genome-wide identification, characterization, and expression analysis related to autotoxicity of the GST gene family in Cucumis melo L

Plant Physiology and Biochemistry, Oct 1, 2020

Glutathione S-transferase (GST) plays an important role in plant resistance to biotic and abiotic... more Glutathione S-transferase (GST) plays an important role in plant resistance to biotic and abiotic stresses. In this paper, the characteristics of melon GST gene family members were analyzed from a genome-wide perspective. Forty-nine GSTs were identified in melon genome, belonging to eight classes. Through the phylogenetic analysis of GST proteins in melon and other plants, it was found that members from the same subfamily in different species clustered together, indicating that the subfamilies of GST have diversified before the divergence within these species. The results of chromosome mapping showed that GSTs were present in all chromosomes except for chromosome 5. Gene replication events played an important role in the expansion and evolution of melon GST gene family. Ten GSTs with significant differential expression were screened in the transcriptome database related to melon autotoxicity stress. The differential expression of these 10 GSTs was detected in roots and leaves of melon seedlings treated with cinnamic acid. The relative expression level of CmGSTU7, CmGSTU10, CmGSTU18, CmGSTF2 and CmGSTL1 in roots of melon seedlings was significantly higher than that in control group. It suggested that the five GSTs might play an important role in cinnamic acid mediated autotoxicity stress in melon. The results of this paper were helpful to reveal the evolution and functional succession of GST family and further understand the response of GST to autotoxicity stress in melon.

Research paper thumbnail of Comparative transcriptome analyses revealed different heat stress responses in high- and low-GS Brassica alboglabra sprouts

BMC Genomics, Apr 4, 2019

Background: Chinese kale (Brassica alboglabra) contains high nutritional elements and functional ... more Background: Chinese kale (Brassica alboglabra) contains high nutritional elements and functional molecules, especially anticarcinogenic and antioxidant glucosinolates (GS), which was highly affected by environment temperature. To investigate the link of GS biosynthesis with heat stress response in Chinese kale, global transcription profiles of high-GS line (HG), low-GS line (LG), high-GS line under heat stress (HGT) and low-GS line under heat stress (LGT) were analyzed. Results: Based on three biological replicates of each RNA sequencing data, 3901, 4062 and 2396 differentially expressed genes in HG vs HGT, LG vs LGT and HGT vs LGT were obtained, respectively. GO annotation, KEGG pathway analysis and a comprehensive analysis of DEGs showed a strong correlation between the GS biosynthesis and heat stress response. It was noticed that 11 differentially expressed genes tied to the GS biosynthesis were down-regulated, 23 heat shock transcription factors and 61 heat shock proteins were up-regulated upon the heat treatment. Another two Chinese kale varieties Cuibao and Shunbao with high-and low-GS content respectively, were used to validate the relationship of GS content and heat-response, and the results showed that high-GS content variety were more thermotolerant than the low-GS content one although GS significantly decreased in both varieties under heat stress. In addition, HSP100/ClpB, HSP90, HSP70 and sHSPs were differentially expressed in high-and low-GS varieties. Notably, HSP90 and sHSPs showed an obviously early response to heat stress than other related genes. Conclusion: The higher heat resistance of high-GS Chinese kale and the sharp decrease of glucosinolate content under heat stress indicated a strong relationship of GS accumulation and heat stress response. Combined with the previous report on the low expression of HSP90 at elevated temperatures in GS-deficient mutant TU8 of Arabidopsis, the differential expression pattern of HSP90 in high-and low-GS varieties and its early heat response implied it might be a key regulator in GS metabolism and heat-resistance in Chinese kale.

Research paper thumbnail of Digital imaging for the evaluation of potato after-cooking darkening: correcting the effect of flesh colour

International Journal of Food Science and Technology, Dec 1, 2009

Research paper thumbnail of Transcriptome profiling of periwinkle infected with Huanglongbing (‘Candidatus Liberibacter asiaticus’)

European Journal of Plant Pathology, Oct 26, 2018

Research paper thumbnail of Chemical triggered defense mechanisms against Phytophthora infestans

Advances in Crop Science and Technology, Jul 21, 2016

L ate blight is the most devastating disease in potato production around the world. It is caused ... more L ate blight is the most devastating disease in potato production around the world. It is caused by Phytophthora infestans which mutates rapidly. Renewed control strategies aimed at higher efficiency and lower environmental impact are urgently required. Research carried out for a decade in our laboratories demonstrated that we can prime potato plants to boost diverse defense functions against Phytophthora infestans. Priming could reduce the use of fungicides and pesticides and ensure the protection of human health and the environment. In this talk, I will present our knowledge about the molecular mechanisms of priming against P. infestans based on our studies using proteomics, metabolomics and gene expression profiling tools. Biography Gefu Wang-Pruski has received her PhD from University of Alberta in Canada and completed her Post doctorate from Loma Linda University in Southern California. She has over 30 year's research experience. She is currently a full Professor at Dalhousie University. She has been leading over 20 national and international research programs in the past 15 years and generated over 50 peer-reviewed publications. She has been serving as an Editorial Board Member for many journals.

Research paper thumbnail of Digital Imaging Analysis - a New Method for Evaluation of Potato After-Cooking Darkening

Research paper thumbnail of Visualization of Intracellular Bacterial Infection in Tumor Cell Lines

Bioluminescence and Chemiluminescence, 2002

Research paper thumbnail of Phosphite uptake and distribution in potato tubers following foliar and postharvest applications of phosphite-based fungicides for late blight control

Annals of Applied Biology, 2016

Research paper thumbnail of Integration of Small RNA and Transcriptome Sequencing Reveal the Roles of miR395 and ATP Sulfurylase in Developing Seeds of Chinese Kale

Frontiers in Plant Science

Seed development is closely related to plant production and reproduction, and MicroRNAs (miRNA) i... more Seed development is closely related to plant production and reproduction, and MicroRNAs (miRNA) is widely involved in plant development including seed development. Chinese kale, as a Brassicaceae vegetable, mainly depends on seed for proper reproduction. In the present study, Chinese kale seed and silique at different stages were selected to establish small RNA (sRNA) libraries including silique wall sRNA libraries at torpedo-embryo stage (PC), silique wall sRNA libraries at cotyledonary-embryo stage (PD), seed sRNA libraries at torpedo-embryo stage (SC), and seed sRNA libraries at cotyledonary-embryo stage (SD). The results showed that miRNA expressed differentially in the seeds and corresponding siliques at different stages. To further clarify the functional mode of miRNA in the process of seed development, Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis was performed on target genes of the differentially expressed miRNAs, and these target genes were mainly enri...

Research paper thumbnail of Irrigation Effect on Yield, Skin Blemishes, Phellem Formation, and Total Phenolics of Red Potatoes

Plants

Dark Red Norland is an important potato cultivar in the fresh market due to its attractive bright... more Dark Red Norland is an important potato cultivar in the fresh market due to its attractive bright, red colour, and good yield. However, skin blemishes such as silver patch, surface cracking, and russeting can negatively influence the tuber skin quality and marketability. It is well known that potato is a drought-sensitive plant. This study was conducted to determine whether irrigation would affect Dark Red Norland’s yield and skin quality. A three-year field trial was conducted by Peak of the Market in Manitoba, Canada. Plants were treated under both irrigation and rainfed conditions. The results show that irrigation increased the total yield by 20.6% and reduced the severity of surface cracking by 48.5%. Microscopy imaging analysis demonstrated that tubers from the rainfed trials formed higher numbers of suberized cell layers than those of the irrigated potatoes, with a difference of 0.360 to 0.652 layers in normal skins. Surface cracking and silver patch skins had more suberized c...

Research paper thumbnail of Aluminum in plant: Benefits, toxicity and tolerance mechanisms

Frontiers in Plant Science

Aluminum (Al) is the third most ubiquitous metal in the earth’s crust. A decrease in soil pH belo... more Aluminum (Al) is the third most ubiquitous metal in the earth’s crust. A decrease in soil pH below 5 increases its solubility and availability. However, its impact on plants depends largely on concentration, exposure time, plant species, developmental age, and growing conditions. Although Al can be beneficial to plants by stimulating growth and mitigating biotic and abiotic stresses, it remains unknown how Al mediates these effects since its biological significance in cellular systems is still unidentified. Al is considered a major limiting factor restricting plant growth and productivity in acidic soils. It instigates a series of phytotoxic symptoms in several Al-sensitive crops with inhibition of root growth and restriction of water and nutrient uptake as the obvious symptoms. This review explores advances in Al benefits, toxicity and tolerance mechanisms employed by plants on acidic soils. These insights will provide directions and future prospects for potential crop improvement.