Gehad Abdel Jaleel - Academia.edu (original) (raw)
Papers by Gehad Abdel Jaleel
Drug development and industrial pharmacy, Apr 13, 2024
Journal of Neurology and Epidemiology, 2017
We investigated the effect of Cannabis sativa extract on brain damage, oxidative stress and infla... more We investigated the effect of Cannabis sativa extract on brain damage, oxidative stress and inflammation in rats with transient global cerebral ischaemia. Rats were subjected to bilateral common carotid artery (CCA) occlusion for 45 minutes followed by 4 h of reperfusion. Rats were treated with cannabis at a dose of 20 mg/kg (expressed as Δ9-THC) intraperitoneally (i.p) either before CCA, at time of reperfusion or after reperfusion. Alternatively, cannabis was given i.p. daily for 2 days before surgery. Markers of oxidative stress (malondialdehyde, reduced glutathione, nitric oxide) and the proinflammatory cytokine tumour necrosis factor-alpha (TNF-α) were determined in brain tissue. Histopathological evaluation was also done. Compared with the sham-treatment group, CCA occlusion resulted in increased brain malondialdehyde (54.5 ± 2.0 vs. 26.0 ± 1.45 nmol/g. tissue; p<0.05) and nitric oxide (75.2 ± 3.2 vs. 31.3 ± 3.0 mmol/g. tissue; p<0.05) contents along with decreased brain ...
Current Organic Synthesis, 2019
Aim and Objective:A series of new 2-pyrazoline analogues were synthesized. The structures of the ... more Aim and Objective:A series of new 2-pyrazoline analogues were synthesized. The structures of the synthesized compounds were elucidated by the analytical and spectroscopic data. Some selected compounds were screened for the anti-inflammatory activity by using animal model of carrageenan-induced paw edema in mice. Additionally, the analgesic and acute toxicity of these compounds were evaluated and exhibited reasonable results. The anti-oxidant and anti-inflammatory effects of these compounds were established by measuring the contents of malondialdehyde (MDA), reduced glutathione (GSH), nitric oxide (NO), and tumor necrosis factor alpha (TNF-α) in the edema paw tissue.Materials and Methods:All chemicals and reagents used in current study were of analytical grade. Melting points were determined using APP. Digital ST 15 melting point apparatus and are uncorrected. FT-IR spectra were recorded on a Pye-Unicam SP3-100 spectrophotometer in KBr pellet. All 1H and 13C NMR spectra were recorded...
Journal of Pharmacology and Toxicology, 2013
Journal of Ethnopharmacology, 2019
Ethnopharmacological relevance: Combretum aculeatum Vent was traditionally used in Sudan, Eretria... more Ethnopharmacological relevance: Combretum aculeatum Vent was traditionally used in Sudan, Eretria and Ethiopia as anti-inflammatory in case of skin inflammation, catarrh, wounds, scorpion stings and snake bites. Nevertheless, there is no scientific information regarding this activity. Aim of study: The present study aimed to evaluate the phytochemical constituents and the scientific basis for the traditional use of Combretum aculeatum Vent through studying its anti-inflammatory properties for the first time to illustrate the putative mechanisms behind this bioactivity. Materials and methods: the ethanolic extract was partitioned by petroleum ether, methylene chloride, ethyl acetate, and n-butanol saturated with water. The petroleum ether fraction was saponified and the saponifiable and unsaponifiable fractions were analyzed on GC/MS. The different fractions were subjected to phytochemical investigation to isolate pure compounds. In-vivo anti-inflammatory activity of the ethanolic extract was evaluated using carrageenan induced rat paws edema method at doses of 200, 400 and 600 mg/kg and proved based on histopathological and biochemical parameters. Results: Five known compounds were isolated for the first time from the aerial parts of Combretum aculeatum Vent: quercetin, vitexin, isorhamnetin 3-O-β-glucoside, isovitexin and rutin, in addition to two previously isolated ones: β-sitosterol and its glucoside. The ethanolic extract evidenced in-vivo anti-inflammatory activity by oral intake of 400 mg/kg of the ethanolic extract significantly (P ≥ 0.05) decreased the paw edema (only 32 ± 1.9% increase in paw weight after 4 h) compared to indomethacin (28.6 ± 2.5%). Moreover, it significantly suppressed the serum malondialdehyde (MDA) and nitric oxide (NO) and increased the GSH to be 11.76 ± 0.85, 5.13 ± 0.62 μmol/mL and 5.66 ± 0.28 μM/mL, respectively. It diminished the serum cytokines TNF-α, IL-6 and IL-1β levels to be 39.1 ± 1.2, 32.6 ± 1.1 and 37.5 ± 1.2 pg/mL, respectively. Results are accompanied by histopathological examination. Conclusion: Overall, the results herein presented significant anti-inflammatory properties traditionally ascribed to Combretum aculeatum Vent. Moreover, the biochemical mechanisms associated to this action were highlighted, introducing new prospects for the development of effective anti-inflammatory herbal medicinal products.
World Academy of Science, Engineering and Technology, International Journal of Pharmacological and Pharmaceutical Sciences, 2017
The present study aimed to investigate the possible effects of kava kava (KK) (Piper methysticum)... more The present study aimed to investigate the possible effects of kava kava (KK) (Piper methysticum) on the hyperglycaemia induced in rats by streptozotocin (STZ; 55 mg/kg; i.p.). Blood samples, were used to determine serum levels of glucose, insulin, total cholesterol (TC) and triglycerides (TG). Some relevant markers for oxidative stress viz., serum lipid peroxides level (measured as malondialdehyde; MDA) and total antioxidant capacity as well as serum nitric oxide (NO) level measured as nitrate/nitrite were determined. Hyperglycaemic animals received orally KK (200 mg/kg) or (500 mg/kg) on daily basis for 28 consecutive days and their effects were determined 24 h after the administration of the last dose. In addition to another group received gliclazide (5 mg/kg; p.o.) as a reference drug. Results of the present study revealed that STZ-induced hyperglycaemia is associated with decreased serum insulin level with increased level of TC and TG. Hyperglycaemia was also associated increas...
Zeitschrift für Naturforschung C, 2016
The fruits of Gleditsia species (Fabaceae) have been known in traditional medicine as a saponin-r... more The fruits of Gleditsia species (Fabaceae) have been known in traditional medicine as a saponin-rich herbal medicine. The present study aimed to investigate the effects of the total methanolic extract of Gleditsia caspica (MEGC) and its saponin-containing fractions (SFGC) on hyperglycemia in streptozotocin (STZ)-induced diabetic rats. A single intraperitoneal injection of STZ (55 mg/kg body weight) was used to induce hyperglycemia in male albino rats. MEGC (15, 30 and 60 mg/kg, p.o.) and SFGC (15, 30 and 60 mg/kg, p.o.) were administered to the diabetic rats daily for 14 days. The anti-diabetic drug gliclazide (10 mg/kg, p.o.) was used as a positive control. Blood samples were collected from overnight fasted rats for the evaluation of the antihyperglycemic, antihyperlipidemic and antioxidant activities. The levels of glucose, triglycerides (TG), cholesterol (TC) and malondialdehyde (MDA) were increased significantly, whereas the levels of α-amylase, insulin and reduced glutathione (...
Canadian Journal of Physiology and Pharmacology, 2014
This study aimed to investigate the possible protective effects of genistein (GEN), a phytoestrog... more This study aimed to investigate the possible protective effects of genistein (GEN), a phytoestrogen, on the liver injury induced in rats by thioacetamide (TTA; 200.0 mg·(kg body mass)–1; administered 3 times a week by intraperitoneal injection). GEN (0.5, 1.0, or 2.0 mg·(kg body mass)–1; by subcutaneous injection) was concurrently administered on a daily basis for 8 weeks, and its effects were evaluated 24 h after the administration of the last dose. The results from this study revealed that TTA-induced liver injury was associated with massive changes in the serum levels of liver biomarkers, oxidative stress markers, and liver inflammatory cytokines. Treatment of TAA-induced liver injury in rats with GEN decreased the elevated serum levels of aspartate aminotransferase, alanine aminotransferase, and total and direct bilirubin, and increased the serum level of albumin. GEN also restored the liver levels of malondialdehyde and reduced glutathione, as well as tumor necrosis factor-alph...
Phytochemistry Letters, 2015
Abstract Phytochemical study on the methanolic extract of Sansevieria cylindrica aerial parts lea... more Abstract Phytochemical study on the methanolic extract of Sansevieria cylindrica aerial parts lead to the isolation, characterization and structure elucidation of a new steroidal saponin, 1β-hydroxy-kryptogenin-1- O -α- l -rhamnopyranosyl-(1 → 2)-α- l -arabinopyranoside ( 1 ), a new homoisoflavanone, (3S)-3,7-dihydroxy-8-methoxy-3-(3′,4′-methylenedioxybenzyl) chroman-4-one ( 2 ) and the known saponin alliospiroside A ( 3 ). To the best of our knowledge, the genin 1β-hydroxy-kryptogenin is reported here for the first time. The structures of the new compounds were determined by UV, IR, EIMS, HRESIMS together with 1D ( 1 H and 13 C) and 2D (HSQC and HMBC) NMR spectral analysis. The isolated compounds 1–3 were tested for their radical scavenging activity (DPPH). Compound 2 exhibited activity compared to that of ascorbic acid as a standard. The cytotoxicity of the isolated compounds and the standard doxorubicin was tested against the three human tumor cell lines HT116, MCF-7 and PC-3. The results showed that the isolated compounds were inactive.
Journal of Food Biochemistry, 2022
Diabetes mellitus has been implicated in the exacerbation of cerebral ischemic injuries. Among th... more Diabetes mellitus has been implicated in the exacerbation of cerebral ischemic injuries. Among the most promising therapeutic approaches is the combination of nutraceuticals and nanotechnology. Curcumin has been termed "the magic molecule", and it was proven to exert several therapeutic actions. Therefore, the aim of the presented work was to investigate the therapeutic effects of curcumin nanoemulsion (NC) administered orally on the middle cerebral artery occlusion and reperfusion (MCAO/Re)-induced cerebral damage in rats with streptozotocin-induced diabetes. The cerebral injury was induced in rats by MCAO/Re 6 weeks after single intraperitoneal STZ injection (50 mg/kg; i.p.). MCAO/Re diabetic rats were then treated with NC (50 and 100 mg/kg; bw; p.o.) for two consecutive weeks. The results of the present study showed that oral treatment of MCAO/Re diabetic rats with NC was associated with a marked attenuation of the neurological deficit score as well as the brain imbalance of the redox homeostasis. NC treatment was also associated with decline in the brain expression of tumor necrosis factor, interleukin-1β, COX-2, cleaved caspase-3, and nuclear factor kappa B. In addition, the expression of glucose transporter 1 proteins upon treatment was restored. PRACTICAL APPLICATIONS: From all these results, it can be concluded that oral supplementation of curcumin nanoemulsion (NC) in diabetic rats reduced the brain injury via augmentation of the expression of glucose transporter 1, as well as its antioxidant and anti-inflammatory properties. Therefore, NC could be delineated as a promising treatment option for cerebral ischemia in diabetic patients.
Biomedicine & Pharmacotherapy, 2021
The study was designed to investigate the potential anti-arthritic effects of methyl palmitate in... more The study was designed to investigate the potential anti-arthritic effects of methyl palmitate in an adjuvant arthritis model in rats that shares many histopathological similarities with human RA. The underlying mechanism and its effect on CD68 macrophages were investigated, as a further argument to its possible efficacy in RA treatment. A normal control group was injected only with saline, arthritic group, and three treatment groups with CFA induced arthritis received methyl palmitate (MP) at three different doses (75, 150, 300 mg/kg/week for 3 weeks, intraperitoneal). The degree of ipsilateral paw swelling, ankle diameter, spleen index, thymus index and the expression levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β were measured. In addition, the underlying molecular mechanism was investigated using CD68 expression. Methyl palpitate significantly and dose dependently decreased the arthritic symptoms as measured by ipsilateral paw volume and ankle diameter. It showed no effect on body weight but significantly decreased splenic, thymus index, serum TNF-α and IL-1β. CD68 macrophages expression and the overall synovial inflammatory cellularity were halted. Methyl palmitate exhibits significant anti-inflammatory and exerts a potential anti-arthritic effect in a rat model of adjuvant induced arthritis. Furthermore, it inhibits expression of synovial CD68 macrophage that validate its therapeutic potential adjuvant arthritis.
Toxicology Reports, 2021
Graphical abstract Dunaliella salina and its isolated Zeaxanthin mitigate counteract age related ... more Graphical abstract Dunaliella salina and its isolated Zeaxanthin mitigate counteract age related dementia via attenuating levels of inflammatory mediators in the brain, i.e. interlekin-1β and inducible nitric oxide synthase, as well as brain Aβ protein and myelin base protein. D. salina overcomes brain aging and thereby repairs age-related dementia, both for its modulating function in attenuating the Aβ protein and neurotransmitters (serotonin, norepinephrine & dopamine).
The aim of this study was to investigate the effect of water extract of dried powdered leaves of ... more The aim of this study was to investigate the effect of water extract of dried powdered leaves of Origanum majorana (O. majorana) on gastric acid secretion and experimental gastric ulcers in rats. Methods: O. majorana was given orally to rats by gastric tube in single doses of 50 mg or 100 mg/kg daily for seven days prior to pylorus ligation or ulcer induction. Gastric acid secretion was determined 4 h after pylorus ligation. Gastric mucosal lesions were produced by oral administration of indomethacin (20 mg/kg) or ethanol (96%, 1 ml). Lipid peroxidation (malondialdehyde), reduced glutathione and the pro-inflammatory cytokines tumour necrosis factor-alpha (TNF-α) and inteuleukin-1beta (IL-1β) were determined in gastric mucosal homogenates. The effects of O. majorana extract were compared with that of the histamine H2-receptor antagonist ranitidine given orally at 50 mg/kg. Results: O. majorana given at 100 mg/kg to pylorus-ligated rats caused a significant decrease in gastric acid o...
Additional file 1: Table S1. Reduction of rats paw edema induced by carrageenan after administrat... more Additional file 1: Table S1. Reduction of rats paw edema induced by carrageenan after administration of tested compounds. Figure S1. Radiological characteristics of hind paws of arthritic rats. Figure S2. Software GOLD 5.2 generated binding mode of indomethacin (left) and naproxen (right) compared to their original co-crystalized conformations. Left: generated binding mode of indomethacin (blue, balls and sticks) in the PDB: 4COX compared to its experimental conformation (black sticks). Right: created binding mode of naproxen (pink, balls and sticks) in the PDB: 3NT1 compared to its co-crystal conformation (black sticks).
Characterization of phytoconstituents in the 70% methanolic extract of Cissus rotundifolia (Forss... more Characterization of phytoconstituents in the 70% methanolic extract of Cissus rotundifolia (Forssk.) Vahl. was performed using high-performance liquid chromatography coupled with electrospray ionization mass spectrometry. Furthermore, tandem mass spectrometry (MS/MS) was performed to assist in the structural elucidation of compounds. Twenty-seven compounds were identified. The identified compounds were four acids, sixteen phenolics, two steroidal saponins, two coumarins, two stilbenoids and one triterpene. Astragalin 1, β-amyrin 2 and 1β-hydroxy-kryptogenin-1-O-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranoside 3 were isolated by column chromatography and their structures were identified by spectral analysis including 1D and 2D NMR. The plant extract and the isolated compounds 1-3 were evaluated for their radical scavenging activity. Compounds 1 and 2 showed significant activity relative to that of ascorbic acid as a standard. The cytotoxicity of the plant extract, the isolated compoun...
International Journal of Pharmacy and Pharmaceutical Sciences, 2016
Objective: Diabetes induces many complications such as cardiovascular problems, cataracts, kidney... more Objective: Diabetes induces many complications such as cardiovascular problems, cataracts, kidney damage and polyneuropathy. Streptozotocin (STZ) induced diabetes is considered one of the most common animal models in rats. The present study investigated the effects of Monascus purpureus (MP) alone or in combination with pioglitazone on glucose level and on liver in streptozotocin (STZ) diabetic rats. Methods: In this study were divided into five experimental groups (normal, untreated STZ-diabetic (60 mg/kg B.W., IP), treated STZ-diabetic with Monascus purpureus (500 mg/kg B. W, oral), treated STZ-diabetic with pioglitazone (10 mg/kg B.W., oral) and treated STZ-diabetic with MP (250 mg/kg B. W, oral)+pioglitazone (10 mg/kg B.W., oral)). Treatment continued for 14 d then blood sampling was done to assess blood glucose. At the end of the study, the animals were fasted overnight, anesthetized with sodium pentobarbital (60 mg/kg i.p.), and sacrificed to collect tissues samples (liver, pa...
International Journal of Pharmacy and Pharmaceutical Sciences, 2016
Objective: This study investigated the possible neuroprotective effects of Ginkgo biloba on cereb... more Objective: This study investigated the possible neuroprotective effects of Ginkgo biloba on cerebral ischemia-reperfusion (I/R) injury in ovariectomized rats. Methods: Rats were randomly allocated into 5 groups as follows: Sham-operated group, Ovariectomized group (OVX), Cerebral I/R injury ovariectomized group (OVX+I/R) and two treated groups given Ginkgo biloba leaves extract in two doses [(OVX+I/R)+Gin50 and (OVX+I/R)+Gin100] for one month starting one month after ovariectomization. Two months after ovariectomization, cerebral I/R injury was induced. 24 h later, blood and brain samples were collected. Results: Ginkgo biloba (50 and100 mg/kg) significantly decreased concentrations of TNF-α, MDA and NO, increased GSH concentration in serum and brain tissue and improved the histopathological pictures of treated rats when compared to ovariectomized cerebral I/R injury group. The effect of Ginkgo biloba high dose was better than the low dose. Conclusion: Thus the study suggests Ginkgo...
Background: Bisphenol A (BPA) is an environmental estrogenic pollutant It is used in the manufact... more Background: Bisphenol A (BPA) is an environmental estrogenic pollutant It is used in the manufacture of plastic products including beverages, dental materials and baby bottles. Exposure to BPA is unavoidable and recognized as major public health risk particularly in developing countries. Critical effects of BPA toxicity mostly occur during fetal development and postnatal development. Zinc (Zn) is an essential element for the endogenous enzymatic antioxidant processes. It is required for cell proliferation, differentiation, normal growth, immune functions and wound healing. Selenium (Se) is also nutritionally essential element with antioxidant potential. It protects brain from oxidative damage in various models of neurodegeneration. Objective: To investigate the influence of postnatal BPA exposure during lactation on the neonates of exposed rats as well as to investigate and compare the possible protective role of Zn and/ or Se against postnatal BPA- induced developmental and neurobe...
Drug development and industrial pharmacy, Apr 13, 2024
Journal of Neurology and Epidemiology, 2017
We investigated the effect of Cannabis sativa extract on brain damage, oxidative stress and infla... more We investigated the effect of Cannabis sativa extract on brain damage, oxidative stress and inflammation in rats with transient global cerebral ischaemia. Rats were subjected to bilateral common carotid artery (CCA) occlusion for 45 minutes followed by 4 h of reperfusion. Rats were treated with cannabis at a dose of 20 mg/kg (expressed as Δ9-THC) intraperitoneally (i.p) either before CCA, at time of reperfusion or after reperfusion. Alternatively, cannabis was given i.p. daily for 2 days before surgery. Markers of oxidative stress (malondialdehyde, reduced glutathione, nitric oxide) and the proinflammatory cytokine tumour necrosis factor-alpha (TNF-α) were determined in brain tissue. Histopathological evaluation was also done. Compared with the sham-treatment group, CCA occlusion resulted in increased brain malondialdehyde (54.5 ± 2.0 vs. 26.0 ± 1.45 nmol/g. tissue; p<0.05) and nitric oxide (75.2 ± 3.2 vs. 31.3 ± 3.0 mmol/g. tissue; p<0.05) contents along with decreased brain ...
Current Organic Synthesis, 2019
Aim and Objective:A series of new 2-pyrazoline analogues were synthesized. The structures of the ... more Aim and Objective:A series of new 2-pyrazoline analogues were synthesized. The structures of the synthesized compounds were elucidated by the analytical and spectroscopic data. Some selected compounds were screened for the anti-inflammatory activity by using animal model of carrageenan-induced paw edema in mice. Additionally, the analgesic and acute toxicity of these compounds were evaluated and exhibited reasonable results. The anti-oxidant and anti-inflammatory effects of these compounds were established by measuring the contents of malondialdehyde (MDA), reduced glutathione (GSH), nitric oxide (NO), and tumor necrosis factor alpha (TNF-α) in the edema paw tissue.Materials and Methods:All chemicals and reagents used in current study were of analytical grade. Melting points were determined using APP. Digital ST 15 melting point apparatus and are uncorrected. FT-IR spectra were recorded on a Pye-Unicam SP3-100 spectrophotometer in KBr pellet. All 1H and 13C NMR spectra were recorded...
Journal of Pharmacology and Toxicology, 2013
Journal of Ethnopharmacology, 2019
Ethnopharmacological relevance: Combretum aculeatum Vent was traditionally used in Sudan, Eretria... more Ethnopharmacological relevance: Combretum aculeatum Vent was traditionally used in Sudan, Eretria and Ethiopia as anti-inflammatory in case of skin inflammation, catarrh, wounds, scorpion stings and snake bites. Nevertheless, there is no scientific information regarding this activity. Aim of study: The present study aimed to evaluate the phytochemical constituents and the scientific basis for the traditional use of Combretum aculeatum Vent through studying its anti-inflammatory properties for the first time to illustrate the putative mechanisms behind this bioactivity. Materials and methods: the ethanolic extract was partitioned by petroleum ether, methylene chloride, ethyl acetate, and n-butanol saturated with water. The petroleum ether fraction was saponified and the saponifiable and unsaponifiable fractions were analyzed on GC/MS. The different fractions were subjected to phytochemical investigation to isolate pure compounds. In-vivo anti-inflammatory activity of the ethanolic extract was evaluated using carrageenan induced rat paws edema method at doses of 200, 400 and 600 mg/kg and proved based on histopathological and biochemical parameters. Results: Five known compounds were isolated for the first time from the aerial parts of Combretum aculeatum Vent: quercetin, vitexin, isorhamnetin 3-O-β-glucoside, isovitexin and rutin, in addition to two previously isolated ones: β-sitosterol and its glucoside. The ethanolic extract evidenced in-vivo anti-inflammatory activity by oral intake of 400 mg/kg of the ethanolic extract significantly (P ≥ 0.05) decreased the paw edema (only 32 ± 1.9% increase in paw weight after 4 h) compared to indomethacin (28.6 ± 2.5%). Moreover, it significantly suppressed the serum malondialdehyde (MDA) and nitric oxide (NO) and increased the GSH to be 11.76 ± 0.85, 5.13 ± 0.62 μmol/mL and 5.66 ± 0.28 μM/mL, respectively. It diminished the serum cytokines TNF-α, IL-6 and IL-1β levels to be 39.1 ± 1.2, 32.6 ± 1.1 and 37.5 ± 1.2 pg/mL, respectively. Results are accompanied by histopathological examination. Conclusion: Overall, the results herein presented significant anti-inflammatory properties traditionally ascribed to Combretum aculeatum Vent. Moreover, the biochemical mechanisms associated to this action were highlighted, introducing new prospects for the development of effective anti-inflammatory herbal medicinal products.
World Academy of Science, Engineering and Technology, International Journal of Pharmacological and Pharmaceutical Sciences, 2017
The present study aimed to investigate the possible effects of kava kava (KK) (Piper methysticum)... more The present study aimed to investigate the possible effects of kava kava (KK) (Piper methysticum) on the hyperglycaemia induced in rats by streptozotocin (STZ; 55 mg/kg; i.p.). Blood samples, were used to determine serum levels of glucose, insulin, total cholesterol (TC) and triglycerides (TG). Some relevant markers for oxidative stress viz., serum lipid peroxides level (measured as malondialdehyde; MDA) and total antioxidant capacity as well as serum nitric oxide (NO) level measured as nitrate/nitrite were determined. Hyperglycaemic animals received orally KK (200 mg/kg) or (500 mg/kg) on daily basis for 28 consecutive days and their effects were determined 24 h after the administration of the last dose. In addition to another group received gliclazide (5 mg/kg; p.o.) as a reference drug. Results of the present study revealed that STZ-induced hyperglycaemia is associated with decreased serum insulin level with increased level of TC and TG. Hyperglycaemia was also associated increas...
Zeitschrift für Naturforschung C, 2016
The fruits of Gleditsia species (Fabaceae) have been known in traditional medicine as a saponin-r... more The fruits of Gleditsia species (Fabaceae) have been known in traditional medicine as a saponin-rich herbal medicine. The present study aimed to investigate the effects of the total methanolic extract of Gleditsia caspica (MEGC) and its saponin-containing fractions (SFGC) on hyperglycemia in streptozotocin (STZ)-induced diabetic rats. A single intraperitoneal injection of STZ (55 mg/kg body weight) was used to induce hyperglycemia in male albino rats. MEGC (15, 30 and 60 mg/kg, p.o.) and SFGC (15, 30 and 60 mg/kg, p.o.) were administered to the diabetic rats daily for 14 days. The anti-diabetic drug gliclazide (10 mg/kg, p.o.) was used as a positive control. Blood samples were collected from overnight fasted rats for the evaluation of the antihyperglycemic, antihyperlipidemic and antioxidant activities. The levels of glucose, triglycerides (TG), cholesterol (TC) and malondialdehyde (MDA) were increased significantly, whereas the levels of α-amylase, insulin and reduced glutathione (...
Canadian Journal of Physiology and Pharmacology, 2014
This study aimed to investigate the possible protective effects of genistein (GEN), a phytoestrog... more This study aimed to investigate the possible protective effects of genistein (GEN), a phytoestrogen, on the liver injury induced in rats by thioacetamide (TTA; 200.0 mg·(kg body mass)–1; administered 3 times a week by intraperitoneal injection). GEN (0.5, 1.0, or 2.0 mg·(kg body mass)–1; by subcutaneous injection) was concurrently administered on a daily basis for 8 weeks, and its effects were evaluated 24 h after the administration of the last dose. The results from this study revealed that TTA-induced liver injury was associated with massive changes in the serum levels of liver biomarkers, oxidative stress markers, and liver inflammatory cytokines. Treatment of TAA-induced liver injury in rats with GEN decreased the elevated serum levels of aspartate aminotransferase, alanine aminotransferase, and total and direct bilirubin, and increased the serum level of albumin. GEN also restored the liver levels of malondialdehyde and reduced glutathione, as well as tumor necrosis factor-alph...
Phytochemistry Letters, 2015
Abstract Phytochemical study on the methanolic extract of Sansevieria cylindrica aerial parts lea... more Abstract Phytochemical study on the methanolic extract of Sansevieria cylindrica aerial parts lead to the isolation, characterization and structure elucidation of a new steroidal saponin, 1β-hydroxy-kryptogenin-1- O -α- l -rhamnopyranosyl-(1 → 2)-α- l -arabinopyranoside ( 1 ), a new homoisoflavanone, (3S)-3,7-dihydroxy-8-methoxy-3-(3′,4′-methylenedioxybenzyl) chroman-4-one ( 2 ) and the known saponin alliospiroside A ( 3 ). To the best of our knowledge, the genin 1β-hydroxy-kryptogenin is reported here for the first time. The structures of the new compounds were determined by UV, IR, EIMS, HRESIMS together with 1D ( 1 H and 13 C) and 2D (HSQC and HMBC) NMR spectral analysis. The isolated compounds 1–3 were tested for their radical scavenging activity (DPPH). Compound 2 exhibited activity compared to that of ascorbic acid as a standard. The cytotoxicity of the isolated compounds and the standard doxorubicin was tested against the three human tumor cell lines HT116, MCF-7 and PC-3. The results showed that the isolated compounds were inactive.
Journal of Food Biochemistry, 2022
Diabetes mellitus has been implicated in the exacerbation of cerebral ischemic injuries. Among th... more Diabetes mellitus has been implicated in the exacerbation of cerebral ischemic injuries. Among the most promising therapeutic approaches is the combination of nutraceuticals and nanotechnology. Curcumin has been termed "the magic molecule", and it was proven to exert several therapeutic actions. Therefore, the aim of the presented work was to investigate the therapeutic effects of curcumin nanoemulsion (NC) administered orally on the middle cerebral artery occlusion and reperfusion (MCAO/Re)-induced cerebral damage in rats with streptozotocin-induced diabetes. The cerebral injury was induced in rats by MCAO/Re 6 weeks after single intraperitoneal STZ injection (50 mg/kg; i.p.). MCAO/Re diabetic rats were then treated with NC (50 and 100 mg/kg; bw; p.o.) for two consecutive weeks. The results of the present study showed that oral treatment of MCAO/Re diabetic rats with NC was associated with a marked attenuation of the neurological deficit score as well as the brain imbalance of the redox homeostasis. NC treatment was also associated with decline in the brain expression of tumor necrosis factor, interleukin-1β, COX-2, cleaved caspase-3, and nuclear factor kappa B. In addition, the expression of glucose transporter 1 proteins upon treatment was restored. PRACTICAL APPLICATIONS: From all these results, it can be concluded that oral supplementation of curcumin nanoemulsion (NC) in diabetic rats reduced the brain injury via augmentation of the expression of glucose transporter 1, as well as its antioxidant and anti-inflammatory properties. Therefore, NC could be delineated as a promising treatment option for cerebral ischemia in diabetic patients.
Biomedicine & Pharmacotherapy, 2021
The study was designed to investigate the potential anti-arthritic effects of methyl palmitate in... more The study was designed to investigate the potential anti-arthritic effects of methyl palmitate in an adjuvant arthritis model in rats that shares many histopathological similarities with human RA. The underlying mechanism and its effect on CD68 macrophages were investigated, as a further argument to its possible efficacy in RA treatment. A normal control group was injected only with saline, arthritic group, and three treatment groups with CFA induced arthritis received methyl palmitate (MP) at three different doses (75, 150, 300 mg/kg/week for 3 weeks, intraperitoneal). The degree of ipsilateral paw swelling, ankle diameter, spleen index, thymus index and the expression levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β were measured. In addition, the underlying molecular mechanism was investigated using CD68 expression. Methyl palpitate significantly and dose dependently decreased the arthritic symptoms as measured by ipsilateral paw volume and ankle diameter. It showed no effect on body weight but significantly decreased splenic, thymus index, serum TNF-α and IL-1β. CD68 macrophages expression and the overall synovial inflammatory cellularity were halted. Methyl palmitate exhibits significant anti-inflammatory and exerts a potential anti-arthritic effect in a rat model of adjuvant induced arthritis. Furthermore, it inhibits expression of synovial CD68 macrophage that validate its therapeutic potential adjuvant arthritis.
Toxicology Reports, 2021
Graphical abstract Dunaliella salina and its isolated Zeaxanthin mitigate counteract age related ... more Graphical abstract Dunaliella salina and its isolated Zeaxanthin mitigate counteract age related dementia via attenuating levels of inflammatory mediators in the brain, i.e. interlekin-1β and inducible nitric oxide synthase, as well as brain Aβ protein and myelin base protein. D. salina overcomes brain aging and thereby repairs age-related dementia, both for its modulating function in attenuating the Aβ protein and neurotransmitters (serotonin, norepinephrine & dopamine).
The aim of this study was to investigate the effect of water extract of dried powdered leaves of ... more The aim of this study was to investigate the effect of water extract of dried powdered leaves of Origanum majorana (O. majorana) on gastric acid secretion and experimental gastric ulcers in rats. Methods: O. majorana was given orally to rats by gastric tube in single doses of 50 mg or 100 mg/kg daily for seven days prior to pylorus ligation or ulcer induction. Gastric acid secretion was determined 4 h after pylorus ligation. Gastric mucosal lesions were produced by oral administration of indomethacin (20 mg/kg) or ethanol (96%, 1 ml). Lipid peroxidation (malondialdehyde), reduced glutathione and the pro-inflammatory cytokines tumour necrosis factor-alpha (TNF-α) and inteuleukin-1beta (IL-1β) were determined in gastric mucosal homogenates. The effects of O. majorana extract were compared with that of the histamine H2-receptor antagonist ranitidine given orally at 50 mg/kg. Results: O. majorana given at 100 mg/kg to pylorus-ligated rats caused a significant decrease in gastric acid o...
Additional file 1: Table S1. Reduction of rats paw edema induced by carrageenan after administrat... more Additional file 1: Table S1. Reduction of rats paw edema induced by carrageenan after administration of tested compounds. Figure S1. Radiological characteristics of hind paws of arthritic rats. Figure S2. Software GOLD 5.2 generated binding mode of indomethacin (left) and naproxen (right) compared to their original co-crystalized conformations. Left: generated binding mode of indomethacin (blue, balls and sticks) in the PDB: 4COX compared to its experimental conformation (black sticks). Right: created binding mode of naproxen (pink, balls and sticks) in the PDB: 3NT1 compared to its co-crystal conformation (black sticks).
Characterization of phytoconstituents in the 70% methanolic extract of Cissus rotundifolia (Forss... more Characterization of phytoconstituents in the 70% methanolic extract of Cissus rotundifolia (Forssk.) Vahl. was performed using high-performance liquid chromatography coupled with electrospray ionization mass spectrometry. Furthermore, tandem mass spectrometry (MS/MS) was performed to assist in the structural elucidation of compounds. Twenty-seven compounds were identified. The identified compounds were four acids, sixteen phenolics, two steroidal saponins, two coumarins, two stilbenoids and one triterpene. Astragalin 1, β-amyrin 2 and 1β-hydroxy-kryptogenin-1-O-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranoside 3 were isolated by column chromatography and their structures were identified by spectral analysis including 1D and 2D NMR. The plant extract and the isolated compounds 1-3 were evaluated for their radical scavenging activity. Compounds 1 and 2 showed significant activity relative to that of ascorbic acid as a standard. The cytotoxicity of the plant extract, the isolated compoun...
International Journal of Pharmacy and Pharmaceutical Sciences, 2016
Objective: Diabetes induces many complications such as cardiovascular problems, cataracts, kidney... more Objective: Diabetes induces many complications such as cardiovascular problems, cataracts, kidney damage and polyneuropathy. Streptozotocin (STZ) induced diabetes is considered one of the most common animal models in rats. The present study investigated the effects of Monascus purpureus (MP) alone or in combination with pioglitazone on glucose level and on liver in streptozotocin (STZ) diabetic rats. Methods: In this study were divided into five experimental groups (normal, untreated STZ-diabetic (60 mg/kg B.W., IP), treated STZ-diabetic with Monascus purpureus (500 mg/kg B. W, oral), treated STZ-diabetic with pioglitazone (10 mg/kg B.W., oral) and treated STZ-diabetic with MP (250 mg/kg B. W, oral)+pioglitazone (10 mg/kg B.W., oral)). Treatment continued for 14 d then blood sampling was done to assess blood glucose. At the end of the study, the animals were fasted overnight, anesthetized with sodium pentobarbital (60 mg/kg i.p.), and sacrificed to collect tissues samples (liver, pa...
International Journal of Pharmacy and Pharmaceutical Sciences, 2016
Objective: This study investigated the possible neuroprotective effects of Ginkgo biloba on cereb... more Objective: This study investigated the possible neuroprotective effects of Ginkgo biloba on cerebral ischemia-reperfusion (I/R) injury in ovariectomized rats. Methods: Rats were randomly allocated into 5 groups as follows: Sham-operated group, Ovariectomized group (OVX), Cerebral I/R injury ovariectomized group (OVX+I/R) and two treated groups given Ginkgo biloba leaves extract in two doses [(OVX+I/R)+Gin50 and (OVX+I/R)+Gin100] for one month starting one month after ovariectomization. Two months after ovariectomization, cerebral I/R injury was induced. 24 h later, blood and brain samples were collected. Results: Ginkgo biloba (50 and100 mg/kg) significantly decreased concentrations of TNF-α, MDA and NO, increased GSH concentration in serum and brain tissue and improved the histopathological pictures of treated rats when compared to ovariectomized cerebral I/R injury group. The effect of Ginkgo biloba high dose was better than the low dose. Conclusion: Thus the study suggests Ginkgo...
Background: Bisphenol A (BPA) is an environmental estrogenic pollutant It is used in the manufact... more Background: Bisphenol A (BPA) is an environmental estrogenic pollutant It is used in the manufacture of plastic products including beverages, dental materials and baby bottles. Exposure to BPA is unavoidable and recognized as major public health risk particularly in developing countries. Critical effects of BPA toxicity mostly occur during fetal development and postnatal development. Zinc (Zn) is an essential element for the endogenous enzymatic antioxidant processes. It is required for cell proliferation, differentiation, normal growth, immune functions and wound healing. Selenium (Se) is also nutritionally essential element with antioxidant potential. It protects brain from oxidative damage in various models of neurodegeneration. Objective: To investigate the influence of postnatal BPA exposure during lactation on the neonates of exposed rats as well as to investigate and compare the possible protective role of Zn and/ or Se against postnatal BPA- induced developmental and neurobe...