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Papers by George Skryabin

Research paper thumbnail of 6-Chloro- and 6-Bromo-Substituted Steroids in the Suzuki-Miyaura Cross-Coupling Reaction. A Convenient Route to Potential Aromatase Inhibitors

Synthesis, 2006

Chlorine at an sp 2-carbon in steroids has been shown to be reactive in Suzuki-Miyaura cross-coup... more Chlorine at an sp 2-carbon in steroids has been shown to be reactive in Suzuki-Miyaura cross-coupling reactions with either Ni or Pd catalysts. The coupling of analogous 6-bromo derivatives has also been demonstrated to be applicable to a wider scope of substrates. The Suzuki-Miyaura arylation of 6-bromo-D 3,5-steroid enol ethers with subsequent hydrolysis is a useful route to 6-arylated steroids bearing aryl at a saturated carbon.

Research paper thumbnail of A Facile Synthesis of 4- and 6-Aryl-Substituted Steroids by the Suzuki-Miyaura­ Cross-Coupling Reaction

Synthesis, 2005

Potential aromatase inhibitors -4- and 6-aryl-substituted derivatives of androst-4-ene-3,17-dione... more Potential aromatase inhibitors -4- and 6-aryl-substituted derivatives of androst-4-ene-3,17-dione and 17-hydroxyprogesterone - have been prepared by the Suzuki-Miyaura cross-coupling from their corresponding 4- and 6-bromosteroids.

Research paper thumbnail of An ELISA Platform for the Quantitative Analysis of SARS-CoV-2 RBD-neutralizing Antibodies As an Alternative to Monitoring of the Virus-Neutralizing Activity

Acta Naturae

Monitoring of the level of the virus-neutralizing activity of serum immunoglobulins ensures that ... more Monitoring of the level of the virus-neutralizing activity of serum immunoglobulins ensures that one can reliably assess the effectiveness of any protection against the SARS-CoV-2 infection. For SARS-CoV-2, the RBD-ACE2 neutralizing activity of sera is almost equivalent to the virus-neutralizing activity of their antibodies and can be used to assess the level of SARS-CoV-2 neutralizing antibodies. We are proposing an ELISA platform for performing a quantitative analysis of SARS-CoV-2 RBD-neutralizing antibodies, as an alternative to the monitoring of the virus-neutralizing activity using pseudovirus or live virus assays. The advantage of the developed platform is that it can be adapted to newly emerging virus variants in a very short time (12 weeks) and, thereby, provide quantitative data on the activity of SARS-CoV-2 RBD-neutralizing antibodies. The developed platform can be used to (1) study herd immunity to SARS-CoV-2, (2) monitor the effectiveness of the vaccination drive (revac...

Research paper thumbnail of Epitope-Specific Response of Human Milk Immunoglobulins in COVID-19 Recovered Women

Pathogens

The breastfeeding of infants by mothers who are infected with SARS-CoV-2 has become a dramatic he... more The breastfeeding of infants by mothers who are infected with SARS-CoV-2 has become a dramatic healthcare problem. The WHO recommends that infected women should not abandon breastfeeding; however, there is still the risk of contact transmission. Convalescent donor milk may provide a defense against the aforementioned issue and can eliminate the consequences of artificial feeding. Therefore, it is vital to characterize the epitope-specific immunological landscape of human milk from women who recovered from COVID-19. We carried out a comprehensive ELISA-based analysis of blood serum and human milk from maternity patients who had recovered from COVID-19 at different trimesters of pregnancy. It was found that patients predominantly contained SARS-CoV-2 N-protein-specific immunoglobulins and had manifested the antibodies for all the antigens tested in a protein-specific and time-dependent manner. Women who recovered from COVID-19 at trimester I–II showed a noticeable decrease in the numb...

Research paper thumbnail of 6-Chloro- and 6-Bromo-Substituted Steroids in the Suzuki-Miyaura Cross-Coupling Reaction. A Convenient Route to Potential Aromatase Inhibitors

Synthesis, 2006

Chlorine at an sp 2-carbon in steroids has been shown to be reactive in Suzuki-Miyaura cross-coup... more Chlorine at an sp 2-carbon in steroids has been shown to be reactive in Suzuki-Miyaura cross-coupling reactions with either Ni or Pd catalysts. The coupling of analogous 6-bromo derivatives has also been demonstrated to be applicable to a wider scope of substrates. The Suzuki-Miyaura arylation of 6-bromo-D 3,5-steroid enol ethers with subsequent hydrolysis is a useful route to 6-arylated steroids bearing aryl at a saturated carbon.

Research paper thumbnail of A Facile Synthesis of 4- and 6-Aryl-Substituted Steroids by the Suzuki-Miyaura­ Cross-Coupling Reaction

Synthesis, 2005

Potential aromatase inhibitors -4- and 6-aryl-substituted derivatives of androst-4-ene-3,17-dione... more Potential aromatase inhibitors -4- and 6-aryl-substituted derivatives of androst-4-ene-3,17-dione and 17-hydroxyprogesterone - have been prepared by the Suzuki-Miyaura cross-coupling from their corresponding 4- and 6-bromosteroids.

Research paper thumbnail of An ELISA Platform for the Quantitative Analysis of SARS-CoV-2 RBD-neutralizing Antibodies As an Alternative to Monitoring of the Virus-Neutralizing Activity

Acta Naturae

Monitoring of the level of the virus-neutralizing activity of serum immunoglobulins ensures that ... more Monitoring of the level of the virus-neutralizing activity of serum immunoglobulins ensures that one can reliably assess the effectiveness of any protection against the SARS-CoV-2 infection. For SARS-CoV-2, the RBD-ACE2 neutralizing activity of sera is almost equivalent to the virus-neutralizing activity of their antibodies and can be used to assess the level of SARS-CoV-2 neutralizing antibodies. We are proposing an ELISA platform for performing a quantitative analysis of SARS-CoV-2 RBD-neutralizing antibodies, as an alternative to the monitoring of the virus-neutralizing activity using pseudovirus or live virus assays. The advantage of the developed platform is that it can be adapted to newly emerging virus variants in a very short time (12 weeks) and, thereby, provide quantitative data on the activity of SARS-CoV-2 RBD-neutralizing antibodies. The developed platform can be used to (1) study herd immunity to SARS-CoV-2, (2) monitor the effectiveness of the vaccination drive (revac...

Research paper thumbnail of Epitope-Specific Response of Human Milk Immunoglobulins in COVID-19 Recovered Women

Pathogens

The breastfeeding of infants by mothers who are infected with SARS-CoV-2 has become a dramatic he... more The breastfeeding of infants by mothers who are infected with SARS-CoV-2 has become a dramatic healthcare problem. The WHO recommends that infected women should not abandon breastfeeding; however, there is still the risk of contact transmission. Convalescent donor milk may provide a defense against the aforementioned issue and can eliminate the consequences of artificial feeding. Therefore, it is vital to characterize the epitope-specific immunological landscape of human milk from women who recovered from COVID-19. We carried out a comprehensive ELISA-based analysis of blood serum and human milk from maternity patients who had recovered from COVID-19 at different trimesters of pregnancy. It was found that patients predominantly contained SARS-CoV-2 N-protein-specific immunoglobulins and had manifested the antibodies for all the antigens tested in a protein-specific and time-dependent manner. Women who recovered from COVID-19 at trimester I–II showed a noticeable decrease in the numb...

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