Gerema Amente - Academia.edu (original) (raw)
Papers by Gerema Amente
Acta Botanica Plantae : 01–09 (2022), 2022
Conventional vegetative propagation of bananas generally has low production, the transmission of ... more Conventional vegetative propagation of bananas generally has low production, the transmission of diseases, and poor preservationof original plant genetic material. Micropropagation is currently the only practical means of achieving rapid, large-scale production of disease-free quality planting material. The present study was conducted to optimize quick and reproducible in vitro micropropagation protocol for three banana varieties (Grandnaine, Poyo, and Butuza) grown in Oromia Experiments on shoot tip culture initiation, shoot multiplication and in vitro rooting were laid out in completely randomized design with 3x2x2, 5x4x3, and 4x3 factorial treatment arrangements respectively. Data were subjected to analysis of variance (ANOVA) and significant means were separated using Duncan's multiple range tests. About shoot multiplication, Grandnaine showed a maximum of 12.67shoots per explant with a 2.17cm shoot length on a medium fortified with 3.5mg/l BAP alone, while Poyo produced a maximum of 13.00shoots per explant with 2.15cm mean shoot length on a medium supplied with 3.0 mg/lBAP and 0.2mg/l IBA. Likewise, Butuza produced a maximum of 11.33 shootsper explant with a mean shoot length 2.9 cm on medium fortified with 4.0 mg/l BAP + 0.3 mg/l IBA. Half MS-semi-solid medium containing 1.0 mg/l IBA induced the highest rooting with 8 mean root numbers per shoot for Grandnaine. For Poyo, half MS medium supplemented with 1.5mg/l IBA induced the highest rooting response with mean root number per shoot of 7.6. Similarly, ½ MS medium supplemented with 2.0 mg/l IBA induced the highest rooting response with 8 mean root numbers per shoot. In conclusion, this study can be used for rapid and massin Vitro propagation of these three elite Banana varieties.
Digital Economy: Complexity and Variety vs. Rationality, 2019
Purpose: The purpose of the research is to substantiate the perspectives of provision of new qual... more Purpose: The purpose of the research is to substantiate the perspectives of provision of new quality of growth of economic systems in the digital economy and to develop the scientific recommendations for their practical implementation.
Journal of Scientific Agriculture
Browning is one of the severe problems in plant tissue culture that hampers successful in vitro p... more Browning is one of the severe problems in plant tissue culture that hampers successful in vitro propagation of plants especially woody and perennial plants. In order to control the browning problem, different efforts has been made in vitro such as presoaking of explants in antioxidant solution, incorporation of antioxidants into medium, culturing in the dark period and frequent subculturing of explants. Presoaking of explants in antioxidant solutions like polyvinylpyrolidone (PvP) and ascorbic acid (AC) is one of the most frequently used. Incorporation of antioxidants such as 0.2-0.5g/l PvP and 15-250mg/l ascorbic acid into MS medium is commonly used to control browning in different plants and explants followed by activated charcoal, citric acid, MES and AIP. Moreover, frequent sub culturing and incubation of explants in the dark period is the other alternative. This review article includes the study of previous and current research achievements in a comprehensive way on the differe...
Browning is one of the severe problems in plant tissue culture that hampers successful in vitro p... more Browning is one of the severe problems in plant tissue culture that hampers successful in vitro propagation of plants especially woody and perennial plants. In order to control the browning problem, different efforts has been made in vitro such as presoaking of explants in antioxidant solution, incorporation of antioxidants in to medium, culturing in the dark period and frequent subculturng of explants. Presoaking of explants in antioxidant solution like polyvinylpyrolidone (PvP) and ascorbic acid (AC) is one of the most frequently used. Incorporation of antioxidants such as 0.2-0.5g/l PvP and 15-250mg/l ascorbic acid in to MS medium are commonly used to control browning in different plants and explants followed by activated charcoal,citrc acid,MES,andAIP. Moreover, frequent sub culturing and incubation of explants in the dark period is the other alternative. This review article includes study of previous and current research achievements in a comprehensive way on the different methods to control browning problem in plant tissue culture and suggests further optimization for successful control of browning when using the same or different crops as well as explants.
Conventional propagation method where nodal sections with two or three nodes or sets are used as ... more Conventional propagation method where nodal sections with two or three nodes or sets are used as planting material, has a wide-range of limitations. Micro propagation is the only practical means of achieving rapid, large-scale production of disease-free quality planting material as seed canes of newly developed varieties in order to speed up the breeding and commercialization process in sugarcane. Thus, the experiment was carried out with the objective of studying the effect of growth regulators in vitro rooting of shoot cultures of three elite sugarcane genotypes. An experiment on in vitro rooting was laid out in completely randomized design with 3x5x3 factorial treatment arrangements. Data was subjected to Analysis of Variance (ANOVA) and significant means were separated using Duncan's multiple range tests. Half MS semi solid medium containing 3.0 mg/l NAA induced the highest rooting with mean root number per shoot of 14.93 for Q200. For co-0238, half MS medium supplemented wi...
Conventional vegetative propagation of sugarcane generally has low multiplication rate and allows... more Conventional vegetative propagation of sugarcane generally has low multiplication rate and allows distribution of diseases. Micropropagation is the only practical means of achieving rapid, large-scale production of disease-free quality planting material. Experiments on shoot tip culture initiation and shoot multiplication were laid out in completely randomized design with 2x3x3 and 4x5x3 factorial treatment arrangements respectively. Data was subjected to analysis of variance (ANOVA) and significant means were separated using Duncan's multiple range tests. With regard to shoot multiplication, genotype Q200 showed a maximum of 13.59 shoots per explant with 5.83cm shoot length on a medium fortified with 2 mg/l BAP alone, while genotype Q217 produced a maximum of 15.28 shoots per explant with 5.37cm mean shoot length on a medium supplied with 2.0 mg/l BAP and 0.25 mg/l kinetin. Likewise, Co-0238 produced maximum of 13.56 shoots per explant with mean shoot length 6.50 cm on medium fortified with 1.5 mg/l BAP + 0.5mg/l kinetin
Degraded forests in …, 2010
... conducted in forest areas managed by the user groups has shown an improvement in the forest c... more ... conducted in forest areas managed by the user groups has shown an improvement in the forest condition and the livelihoods of the people (Bekele et al ... The distribution of potential crop trees is in agreement with studies conducted in Dindin forest (Shibru and Balcha, 2004). ...
Conventional vegetative propagation of sugarcane generally has low multiplication rate and allows... more Conventional vegetative propagation of sugarcane generally has low multiplication rate and allows distribution of diseases. Micropropagation is the only practical means of achieving rapid, large-scale production of disease-free quality planting material. Experiments on shoot tip culture initiation and shoot multiplication were laid out in completely randomized design with 2x3x3 and 4x5x3 factorial treatment arrangements respectively. Data was subjected to analysis of variance (ANOVA) and significant means were separated using Duncan's multiple range tests. With regard to shoot multiplication, genotype Q200 showed a maximum of 13.59 shoots per explant with 5.83cm shoot length on a medium fortified with 2 mg/l BAP alone, while genotype Q217 produced a maximum of 15.28 shoots per explant with 5.37cm mean shoot length on a medium supplied with 2.0 mg/l BAP and 0.25 mg/l kinetin. Likewise, Co-0238 produced maximum of 13.56 shoots per explant with mean shoot length 6.50 cm on medium f...
Soil Science Society of America Journal, 2000
... to measure. Solution samplers are often employed, but ... The contribution of the solid fract... more ... to measure. Solution samplers are often employed, but ... The contribution of the solid fraction in situ measurement of ECb offers the promise of im-... The predominant path is through the soil solu-tion, ECw, also known as pore water electrical conductivity. Se h hcr [6] ...
Acta Botanica Plantae : 01–09 (2022), 2022
Conventional vegetative propagation of bananas generally has low production, the transmission of ... more Conventional vegetative propagation of bananas generally has low production, the transmission of diseases, and poor preservationof original plant genetic material. Micropropagation is currently the only practical means of achieving rapid, large-scale production of disease-free quality planting material. The present study was conducted to optimize quick and reproducible in vitro micropropagation protocol for three banana varieties (Grandnaine, Poyo, and Butuza) grown in Oromia Experiments on shoot tip culture initiation, shoot multiplication and in vitro rooting were laid out in completely randomized design with 3x2x2, 5x4x3, and 4x3 factorial treatment arrangements respectively. Data were subjected to analysis of variance (ANOVA) and significant means were separated using Duncan's multiple range tests. About shoot multiplication, Grandnaine showed a maximum of 12.67shoots per explant with a 2.17cm shoot length on a medium fortified with 3.5mg/l BAP alone, while Poyo produced a maximum of 13.00shoots per explant with 2.15cm mean shoot length on a medium supplied with 3.0 mg/lBAP and 0.2mg/l IBA. Likewise, Butuza produced a maximum of 11.33 shootsper explant with a mean shoot length 2.9 cm on medium fortified with 4.0 mg/l BAP + 0.3 mg/l IBA. Half MS-semi-solid medium containing 1.0 mg/l IBA induced the highest rooting with 8 mean root numbers per shoot for Grandnaine. For Poyo, half MS medium supplemented with 1.5mg/l IBA induced the highest rooting response with mean root number per shoot of 7.6. Similarly, ½ MS medium supplemented with 2.0 mg/l IBA induced the highest rooting response with 8 mean root numbers per shoot. In conclusion, this study can be used for rapid and massin Vitro propagation of these three elite Banana varieties.
Digital Economy: Complexity and Variety vs. Rationality, 2019
Purpose: The purpose of the research is to substantiate the perspectives of provision of new qual... more Purpose: The purpose of the research is to substantiate the perspectives of provision of new quality of growth of economic systems in the digital economy and to develop the scientific recommendations for their practical implementation.
Journal of Scientific Agriculture
Browning is one of the severe problems in plant tissue culture that hampers successful in vitro p... more Browning is one of the severe problems in plant tissue culture that hampers successful in vitro propagation of plants especially woody and perennial plants. In order to control the browning problem, different efforts has been made in vitro such as presoaking of explants in antioxidant solution, incorporation of antioxidants into medium, culturing in the dark period and frequent subculturing of explants. Presoaking of explants in antioxidant solutions like polyvinylpyrolidone (PvP) and ascorbic acid (AC) is one of the most frequently used. Incorporation of antioxidants such as 0.2-0.5g/l PvP and 15-250mg/l ascorbic acid into MS medium is commonly used to control browning in different plants and explants followed by activated charcoal, citric acid, MES and AIP. Moreover, frequent sub culturing and incubation of explants in the dark period is the other alternative. This review article includes the study of previous and current research achievements in a comprehensive way on the differe...
Browning is one of the severe problems in plant tissue culture that hampers successful in vitro p... more Browning is one of the severe problems in plant tissue culture that hampers successful in vitro propagation of plants especially woody and perennial plants. In order to control the browning problem, different efforts has been made in vitro such as presoaking of explants in antioxidant solution, incorporation of antioxidants in to medium, culturing in the dark period and frequent subculturng of explants. Presoaking of explants in antioxidant solution like polyvinylpyrolidone (PvP) and ascorbic acid (AC) is one of the most frequently used. Incorporation of antioxidants such as 0.2-0.5g/l PvP and 15-250mg/l ascorbic acid in to MS medium are commonly used to control browning in different plants and explants followed by activated charcoal,citrc acid,MES,andAIP. Moreover, frequent sub culturing and incubation of explants in the dark period is the other alternative. This review article includes study of previous and current research achievements in a comprehensive way on the different methods to control browning problem in plant tissue culture and suggests further optimization for successful control of browning when using the same or different crops as well as explants.
Conventional propagation method where nodal sections with two or three nodes or sets are used as ... more Conventional propagation method where nodal sections with two or three nodes or sets are used as planting material, has a wide-range of limitations. Micro propagation is the only practical means of achieving rapid, large-scale production of disease-free quality planting material as seed canes of newly developed varieties in order to speed up the breeding and commercialization process in sugarcane. Thus, the experiment was carried out with the objective of studying the effect of growth regulators in vitro rooting of shoot cultures of three elite sugarcane genotypes. An experiment on in vitro rooting was laid out in completely randomized design with 3x5x3 factorial treatment arrangements. Data was subjected to Analysis of Variance (ANOVA) and significant means were separated using Duncan's multiple range tests. Half MS semi solid medium containing 3.0 mg/l NAA induced the highest rooting with mean root number per shoot of 14.93 for Q200. For co-0238, half MS medium supplemented wi...
Conventional vegetative propagation of sugarcane generally has low multiplication rate and allows... more Conventional vegetative propagation of sugarcane generally has low multiplication rate and allows distribution of diseases. Micropropagation is the only practical means of achieving rapid, large-scale production of disease-free quality planting material. Experiments on shoot tip culture initiation and shoot multiplication were laid out in completely randomized design with 2x3x3 and 4x5x3 factorial treatment arrangements respectively. Data was subjected to analysis of variance (ANOVA) and significant means were separated using Duncan's multiple range tests. With regard to shoot multiplication, genotype Q200 showed a maximum of 13.59 shoots per explant with 5.83cm shoot length on a medium fortified with 2 mg/l BAP alone, while genotype Q217 produced a maximum of 15.28 shoots per explant with 5.37cm mean shoot length on a medium supplied with 2.0 mg/l BAP and 0.25 mg/l kinetin. Likewise, Co-0238 produced maximum of 13.56 shoots per explant with mean shoot length 6.50 cm on medium fortified with 1.5 mg/l BAP + 0.5mg/l kinetin
Degraded forests in …, 2010
... conducted in forest areas managed by the user groups has shown an improvement in the forest c... more ... conducted in forest areas managed by the user groups has shown an improvement in the forest condition and the livelihoods of the people (Bekele et al ... The distribution of potential crop trees is in agreement with studies conducted in Dindin forest (Shibru and Balcha, 2004). ...
Conventional vegetative propagation of sugarcane generally has low multiplication rate and allows... more Conventional vegetative propagation of sugarcane generally has low multiplication rate and allows distribution of diseases. Micropropagation is the only practical means of achieving rapid, large-scale production of disease-free quality planting material. Experiments on shoot tip culture initiation and shoot multiplication were laid out in completely randomized design with 2x3x3 and 4x5x3 factorial treatment arrangements respectively. Data was subjected to analysis of variance (ANOVA) and significant means were separated using Duncan's multiple range tests. With regard to shoot multiplication, genotype Q200 showed a maximum of 13.59 shoots per explant with 5.83cm shoot length on a medium fortified with 2 mg/l BAP alone, while genotype Q217 produced a maximum of 15.28 shoots per explant with 5.37cm mean shoot length on a medium supplied with 2.0 mg/l BAP and 0.25 mg/l kinetin. Likewise, Co-0238 produced maximum of 13.56 shoots per explant with mean shoot length 6.50 cm on medium f...
Soil Science Society of America Journal, 2000
... to measure. Solution samplers are often employed, but ... The contribution of the solid fract... more ... to measure. Solution samplers are often employed, but ... The contribution of the solid fraction in situ measurement of ECb offers the promise of im-... The predominant path is through the soil solu-tion, ECw, also known as pore water electrical conductivity. Se h hcr [6] ...