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Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology, 2017
This study aimed to analyze the effect of low-level laser on the osteoclastogenic potential of a ... more This study aimed to analyze the effect of low-level laser on the osteoclastogenic potential of a squamous cell carcinoma cell line (SCC9). Irradiated SCC9 cells (660 /780 nm; 40 /70 mW; 2, 4 and 6 J/cm 2) were cultured with human osteoclast precursors (PBMC). Co-cultures with non-irradiated SCC9 cells served as control. After 7, 14 and 21 days, the co-cultures (treated or not with specific inhibitors of MEK, NFkB, p38 and JNK pathways) were evaluated for the tartrate-resistant acid phosphatase (TRAP) activity (commercial kit). Monocultures of SCC9 cells (irradiated or not) were analyzed for the expression of IL-11 and PTHrP (by PCR). Irradiated SCC9 cells+PBMC co-cultures presented a significant reduction in TRAP activity (especially with 780 nm, 70 mW irradiation). Regardless the intracellular signaling pathway inhibitor, the decrease in TRAP activity observed in the co-cultures involving irradiated SCC9 cells was more notorious with 780 nm-4 J/cm 2-70 mW. SCC9 cells irradiated with a low output power (780 nm-4 J/cm 2-40 mW and 660 nm-4 J/cm 2-40 mW) and cultured for 2 days presented increased expression of IL-11 and PTHrP. In 9 days cultures, expression of these molecules was lower than in non-irradiated cells. Laser irradiation (780 nm/70 mw/4 J/cm2) of SCC9 cells is able to decrease the pro-osteoclastogenic potential of those cells.
Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology, 2017
region, regarding the oral lesions. Being a typical rural region with high levels of solar radiat... more region, regarding the oral lesions. Being a typical rural region with high levels of solar radiation, large population of smokers and drinkers, the oral lesions more prevalent are associated with exposure cited as cheilitis, inflammatory hyperplasia, leukoplakia and various carcinomas. The survey will serve for planning actions aimed at preventing the most prevalent injuries, promotion activities and early detection by the professionals of oral health teams.
Revista Mundi Saúde e Biológicas (ISSN: 2525-4766), 2016
A notificação de casos de violência pelos profissionais de saúde contribui para o dimensionamento... more A notificação de casos de violência pelos profissionais de saúde contribui para o dimensionamento epidemiológico do problema, permitindo o desenvolvimento de programas e ações específicas. O setor de saúde não pode assumir a responsabilidade no combate a violência, entretanto, cabe a ele o envolvimento institucional, de modo a capacitar seus profissionais para o enfrentamento do problema ressaltado na compreensão nas relações sociais conflituosas. O objetivo do trabalho foi desenvolver um material pedagógico para o ensino do preenchimento dos itens 52, 57 e 58 da ficha de notificação do Sistema de Informações de Agravos e Notificação sobre violências por meio de uma linguagem clara e objetiva, utilizando-se de ilustrações. Assim foi realizado uma revisão de literatura para embasar o desenvolvimento do material. O manual foi composto por 50 páginas ilustradas, coloridas, com ampla visualização, encadernado em espiral para um melhor manuseio. O layout foi elaborado para que o leitor t...
Revista Brasileira de Ciência do Solo, 2013
Phosphorus fertilization and irrigation increase coffee production, but little is known about the... more Phosphorus fertilization and irrigation increase coffee production, but little is known about the effect of these practices on soil organic matter and soil microbiota in the Cerrado. The objective of this study was to evaluate the microbiological and oxidizable organic carbon fractions of a dystrophic Red Latossol under coffee and split phosphorus (P) applications and different irrigation regimes. The experiment was arranged in a randomized block design in a 3 x 2 factorial design with three split P applications (P1: 300 kg ha-1 P2O5, recommended for the crop year, of which two thirds were applied in September and the third part in December; P2: 600 kg ha-1 P2O5, applied at planting and then every two years, and P3: 1,800 kg ha-1 P2O5, the requirement for six years, applied at once at planting), two irrigation regimes (rainfed and year-round irrigation), with three replications. The layers 0-5 and 5-10 cm were sampled to determine microbial biomass carbon (MBC), basal respiration (B...
Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology, 2017
This study aimed to analyze the effect of low-level laser on the osteoclastogenic potential of a ... more This study aimed to analyze the effect of low-level laser on the osteoclastogenic potential of a squamous cell carcinoma cell line (SCC9). Irradiated SCC9 cells (660 /780 nm; 40 /70 mW; 2, 4 and 6 J/cm 2) were cultured with human osteoclast precursors (PBMC). Co-cultures with non-irradiated SCC9 cells served as control. After 7, 14 and 21 days, the co-cultures (treated or not with specific inhibitors of MEK, NFkB, p38 and JNK pathways) were evaluated for the tartrate-resistant acid phosphatase (TRAP) activity (commercial kit). Monocultures of SCC9 cells (irradiated or not) were analyzed for the expression of IL-11 and PTHrP (by PCR). Irradiated SCC9 cells+PBMC co-cultures presented a significant reduction in TRAP activity (especially with 780 nm, 70 mW irradiation). Regardless the intracellular signaling pathway inhibitor, the decrease in TRAP activity observed in the co-cultures involving irradiated SCC9 cells was more notorious with 780 nm-4 J/cm 2-70 mW. SCC9 cells irradiated with a low output power (780 nm-4 J/cm 2-40 mW and 660 nm-4 J/cm 2-40 mW) and cultured for 2 days presented increased expression of IL-11 and PTHrP. In 9 days cultures, expression of these molecules was lower than in non-irradiated cells. Laser irradiation (780 nm/70 mw/4 J/cm2) of SCC9 cells is able to decrease the pro-osteoclastogenic potential of those cells.
Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology, 2017
region, regarding the oral lesions. Being a typical rural region with high levels of solar radiat... more region, regarding the oral lesions. Being a typical rural region with high levels of solar radiation, large population of smokers and drinkers, the oral lesions more prevalent are associated with exposure cited as cheilitis, inflammatory hyperplasia, leukoplakia and various carcinomas. The survey will serve for planning actions aimed at preventing the most prevalent injuries, promotion activities and early detection by the professionals of oral health teams.
Revista Mundi Saúde e Biológicas (ISSN: 2525-4766), 2016
A notificação de casos de violência pelos profissionais de saúde contribui para o dimensionamento... more A notificação de casos de violência pelos profissionais de saúde contribui para o dimensionamento epidemiológico do problema, permitindo o desenvolvimento de programas e ações específicas. O setor de saúde não pode assumir a responsabilidade no combate a violência, entretanto, cabe a ele o envolvimento institucional, de modo a capacitar seus profissionais para o enfrentamento do problema ressaltado na compreensão nas relações sociais conflituosas. O objetivo do trabalho foi desenvolver um material pedagógico para o ensino do preenchimento dos itens 52, 57 e 58 da ficha de notificação do Sistema de Informações de Agravos e Notificação sobre violências por meio de uma linguagem clara e objetiva, utilizando-se de ilustrações. Assim foi realizado uma revisão de literatura para embasar o desenvolvimento do material. O manual foi composto por 50 páginas ilustradas, coloridas, com ampla visualização, encadernado em espiral para um melhor manuseio. O layout foi elaborado para que o leitor t...
Revista Brasileira de Ciência do Solo, 2013
Phosphorus fertilization and irrigation increase coffee production, but little is known about the... more Phosphorus fertilization and irrigation increase coffee production, but little is known about the effect of these practices on soil organic matter and soil microbiota in the Cerrado. The objective of this study was to evaluate the microbiological and oxidizable organic carbon fractions of a dystrophic Red Latossol under coffee and split phosphorus (P) applications and different irrigation regimes. The experiment was arranged in a randomized block design in a 3 x 2 factorial design with three split P applications (P1: 300 kg ha-1 P2O5, recommended for the crop year, of which two thirds were applied in September and the third part in December; P2: 600 kg ha-1 P2O5, applied at planting and then every two years, and P3: 1,800 kg ha-1 P2O5, the requirement for six years, applied at once at planting), two irrigation regimes (rainfed and year-round irrigation), with three replications. The layers 0-5 and 5-10 cm were sampled to determine microbial biomass carbon (MBC), basal respiration (B...