Ghorban Nematzadeh - Academia.edu (original) (raw)

Papers by Ghorban Nematzadeh

Trachyspermum copticum (Apiaceae) is an annual plant which grows in Iran. The fruits of T. coptic... more Trachyspermum copticum (Apiaceae) is an annual plant which grows in Iran. The fruits of T. copticum (Ajwain) traditionally were used as diuretic, carminative, and antihelmentic. Some biological effects of Ajwain such as antiviral, antifungal and antioxidant activities have been confirmed. The objective of the present investigation was toevaluate the antibacterial activity of extracts of callus and seed and essential oil of Ajwain against some bacterial strains (Pseudomonas viridiflava, Pseudomonas syringae pv. syringaeand Escherichia coli).The extracts and essential oil were prepared and the antibacterial activity was evaluated via growth inhibitory zone assay using disc diffusion agar technique. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) was measured by micro broth dilution assay. The results revealed no significant effect for callus extract, however, the effect of seed extract and essential oil on tested bacterial strains was statistically ...

Scientific Reports

The study of salt tolerance mechanisms in halophyte plants can provide valuable information for c... more The study of salt tolerance mechanisms in halophyte plants can provide valuable information for crop breeding and plant engineering programs. The aim of the present study was to investigate whole transcriptome analysis of Aeluropus littoralis in response to salinity stress (200 and 400 mM NaCl) by de novo RNA-sequencing. To assemble the transcriptome, Trinity v2.4.0 and Bridger tools, were comparatively used with two k-mer sizes (25 and 32 bp). The de novo assembled transcriptome by Bridger (k-mer 32) was chosen as final assembly for subsequent analysis. In general, 103290 transcripts were obtained. The differential expression analysis (log2FC > 1 and FDR

Background The plant essential oils and extracts are possible sources of natural bioactive molecu... more Background The plant essential oils and extracts are possible sources of natural bioactive molecules and have been selected globally for new antimicrobial compounds, food preservatives, and alternatives to treat infectious disease. Methods In this research, antimicrobial activities of chloroformic and methanolic extracts of Sophora flavescens , Rhaponticum repens , Alhagi maurorum , Melia azedarach , Peganum harmala , and Juncus conglomeratus were evaluated against 8 bacteria ( S. aureus , B. subtilis , R. toxicus , P. aeruginosa , E. coli , P. syringae , X. campestris , P. viridiflava ) and 3 fungi ( Pyricularia oryzae , Fusarium oxysporum and Botrytis cinerea ) through disc diffusion method. Furthermore, the essential oil of plants with the highest antibacterial activity was analyzed utilizing GC/MS. Moreover, tested plants were exposed to screening for possible antioxidant effect utilizing DPPH test, guaiacol peroxidas, and catalase enzymes. Besides, the amount of total phenol an...

International Journal of Systematic and Evolutionary Microbiology

BMC Chemistry

Background: As possible sources of natural bioactive molecules, the plant essential oils and extr... more Background: As possible sources of natural bioactive molecules, the plant essential oils and extracts have been used globally in new antimicrobial compounds, food preservatives, and alternatives to treat infectious disease. Methods: In this research, the antimicrobial activities of chloroformic and methanolic extracts of Sophora flavescens, Rhaponticum repens, Alhagi maurorum, Melia azedarach, Peganum harmala, and Juncus conglomeratus were evaluated against 8 bacteria (S. aureus, B. subtilis, R. toxicus, P. aeruginosa, E. coli, P. syringae, X. campestris, P. viridiflava) and 3 fungi (Pyricularia oryzae, Fusarium oxysporum and Botrytis cinerea), through disc diffusion method. Furthermore, the essential oils of plants with the highest antibacterial activity were analyzed utilizing GC/MS. Moreover, the tested plants were exposed to screening for possible antioxidant effect utilizing DPPH test, guaiacol peroxidas, and catalase enzymes. Besides, the amount of total phenol and flavonoid of these plants was measured. Results: Among the tested plants, methanolic and chloroformic extracts of P. harmala fruits showed the highest antibacterial activity against the tested bacteria. Besides, the investigation of free radical scavenging effects of the tested plants indicated the highest DPPH, protein, guaiacol peroxidase, and catalase in P. harmala, M. azedarach, J. conglomeratus fruits, and J. conglomeratus fruits, respectively. In addition, the phytochemical analysis demonstrated the greatest amounts of total phenolic and flavonoid compositions in J. conglomeratus and P. harmala, respectively. Conclusion: The results indicated that these plants could act as a promising antimicrobial agent, due to their short killing time.

Journal of Essential Oil Bearing Plants

Rice

Background: Salinity expansion in arable land is a threat to crop plants. Rice is the staple food... more Background: Salinity expansion in arable land is a threat to crop plants. Rice is the staple food crop across several countries worldwide; however, its salt sensitive nature severely affects its growth under excessive salinity. FL478 is a salt tolerant indica recombinant inbred line, which can be a good source of salt tolerance at the seedling stage in rice. To learn about the genetic basis of its tolerance to salinity, we compared transcriptome profiles of FL478 and its sensitive parent (IR29) using RNA-seq technique. Results: A total of 1714 and 2670 genes were found differentially expressed (DEGs) under salt stress compared to normal conditions in FL478 and IR29, respectively. Gene ontology analysis revealed the enrichment of transcripts involved in salinity response, regulation of gene expression, and transport in both genotypes. Comparative transcriptome analysis revealed that 1063 DEGs were co-expressed, while 338/252 and 572/908 DEGs were exclusively up/down-regulated in FL478 and IR29, respectively. Further, some biological processes (e.g. iron ion transport, response to abiotic stimulus, and oxidative stress) and molecular function terms (e.g. zinc ion binding and cation transmembrane transporter activity) were specifically enriched in FL478 up-regulated transcripts. Based on the metabolic pathways analysis, genes encoding transport and major intrinsic proteins transporter superfamily comprising aquaporin subfamilies and genes involved in MAPK signaling and signaling receptor kinases were specifically enriched in FL478. A total of 1135 and 1894 alternative splicing events were identified in transcripts of FL478 and IR29, respectively. Transcripts encoding two potassium transporters and two major facilitator family transporters were specifically up-regulated in FL478 under salt stress but not in the salt sensitive genotype. Remarkably, 11 DEGs were conversely regulated in the studied genotypes; for example, OsZIFL, OsNAAT, OsGDSL, and OsELIP genes were up-regulated in FL478, while they were down-regulated in IR29. Conclusions: The achieved results suggest that FL478 employs more efficient mechanisms (especially in signal transduction of salt stress, influx and transport of k + , ionic and osmotic homeostasis, as well as ROS inhibition) to respond to the salt stress compared to its susceptible parent.

Horticulture International Journal

In this study, the ability Inter Simple Sequence Repeat markers (ISSR) were used to identify mole... more In this study, the ability Inter Simple Sequence Repeat markers (ISSR) were used to identify molecular relationships among wild, local and commercial genotypes of strawberry. Twelve out of the 25 tested primers produced clear and reproducible fragments with high value of polymorphism (96.5%). The number of bands per primer ranged from 6 to 18 with an average of 13.16 bands. Clustering based on Jaccard's similarity coefficient divided the genotypes into five groups and separated wild accessions from others. The highest genetic distance was between Kaleybar and Pahnekola sari accessions with 0.31 similarity index that is promising to obtain hybrids with greater heterotic effect and segregation. The results indicated that there are high value of divergence between the genotypes and clearly demonstrate that ISSR markers can be used in a genetic diversity studying as well as in genotypic identification of strawberries. The results of the study can be use for further analysis, including fingerprinting, preservation of genetic resources, parental selection and hybrid/cross-breeding programs.

Plant Systematics and Evolution

Journal of Chromatography B

Journal of Pure and Applied Microbiology, Apr 1, 2014

Maintenance of internal status of K + ion is one of the important mechanisms for regulation of po... more Maintenance of internal status of K + ion is one of the important mechanisms for regulation of potassium balance under salt stress. In the present study, we use Aeluropus littoralis as a halophytic monocot model to investigate transcriptional changes in HAK1 gene that code a H +-K + transporter for acquire potassium under condition of limited availability of K + and also measurement of some physiological parameters such as Dry weight, Ash and K + content, Photosynthetic pigments and Carbohydrate content under salt stress. The Comparison of HAK1 gene expression in pattern in shoot and root revealed that transcript levels of HAK1 in shoot induced by salt androot contained less levels of HAK1 mRNA than shoot at high concentrations of NaCl. Our results suggest that HAK1type transporters can play an important role in potassium uptake and cell internal diffusion of K + ion to tissue that can be effective in salt tolerance in this grass.

African Journal of Agricultural Research, Sep 18, 2010

... Full Length Research Paper Radio sensitivity studies of morpho-physiological characteristics ... more ... Full Length Research Paper Radio sensitivity studies of morpho-physiological characteristics in some Iranian rice varieties (Oryza sativa L.) in M1 generation Alireza Babaei1*, Ghorban Ali Nematzadeh2, Viacheslav Avagyan3, Seyyed Hamidreza Hashemi-Petrodi2 ...

Grain size is one of the most effective characters in rice (Oryza sativa L.) yield; meanwhile it ... more Grain size is one of the most effective characters in rice (Oryza sativa L.) yield; meanwhile it is a highly important quality trait as well. It is problematic for breeders to improve grain size in early stage of growth. Marker assisted selection (MAS) has an essential role in improvement of quantitative and qualitative traits in crops. Functional markers based on polymorphic loci within genes that directly affecting phenotypic variation improves the quality of MAS process. GS3 is a quantitative trait loci (QTL) in pericentromeric region of chromosome 3 is reported to be responsible for 80-90% of the variation in grain length and weight and the minor QTL for grain width and thickness in rice. In this investigation, the usefulness of a PCR-based SNP marker as a functional SNP in grain size was evaluated. A collection of 100 diverse rice genotypes were planted in 3 replicated lattice design and grain size characters (grain length, width and thickness) were measured. By Analysis of this SNP marker two alleles were detected. ANOVA shows a significant difference in grain size characters between these two groups of alleles. Results indicate that PCR-based SNP marker could discriminate the long and short grain; hence it could be an appropriate, simple and commodious approach for improvement of rice grain size via MAS of GS3 QTL.

Mutation induction has become a proven way for creating variation within a crop variety. The mole... more Mutation induction has become a proven way for creating variation within a crop variety. The molecular behavior of 22 morphological selected M 2 generations of Sange-tarom and Taromhashemi rice cultivars (Oryza sativa L.) evaluated using 26 RAPD molecular markers. Sixty one (57%) and 53 (52.5%) polymorphic bands out of 107 and 101 observed band belonged to Sangetarom and Tarom-hashemi respectively. The maximum produced band (15 bonds) related to the OPD11 and the minimum (3 bonds) belonged to the OPH14 RAPD primer, so the average of bands for each primer is almost 6.5. The size of fragments ranged from 300 bp to 1.8 kbp. The result of this experiment is shown that OPA13 and OPH05 primers are most suitable ones due to their high resolution. The highest and least similarity coefficient between Sange-tarom mutant genotypes and control were S78 (87%), and S58 (39%) and also in Tarom-hashemi genotypes were T58 (63%) and T33 (40%) respectively. On the basis of RAPD analysis and dendrogram resulted, genotypes were classified in 3 groups regarding their similarities and distinctness. So the conclusion of this study revealed sufficient enough variation for rice improvement through gamma ray mutagen.

Mutation breeding has been used to develop numerous plant varieties in recent decades. This paper... more Mutation breeding has been used to develop numerous plant varieties in recent decades. This paper describes an investigation into the scope for using gamma irradiation to create genetic variation in three M 2 generation Iranian rice cultivars (Sange-tarom, Tarom-hashemi and Nemat), using intron-exon splice junctions (ISJ) and analysis of morphological traits to identify mutants. The M 2 genotypes were screened using 21 semi-random primers and 10 morphological traits. Of the 165 bands amplified by the semi-random primers, 67% were polymorphic. The average number of bands per primer and genotype were 12.3 and 11.0, respectively. The size of the amplified fragments ranged from 250 bp to 1.8 kbp. The most informative ISJ primers were IT 31-15 and IT 35-15 . The genetic similarity between control and mutant plants ranged from 6 to 93%, 36.4 to 96.8% and 17.4 to 95% in the Nemat, Sange-tarom and Tarom-hashemi M 2 generations, respectively. Cluster analysis based on the Dice similarity coe...