Giampiero Gualandi - Academia.edu (original) (raw)

Papers by Giampiero Gualandi

Toxicology and Applied Pharmacology, 2009

We have shown that melatonin immediately and transiently stimulates intracellular free radical pr... more We have shown that melatonin immediately and transiently stimulates intracellular free radical production on a set of leukocytes, possibly as a consequence of calmodulin binding. We show here that melatonininduced ROS are produced by lipoxygenase (LOX), since they are prevented by a set of LOX inhibitors, and are accompanied by increase of the 5-LOX product 5-HETE. LOX activation is accompanied by strong liberation of AA; inhibition of Ca 2+-independent, but not Ca 2+-dependent, phospholipase A2 (PLA2), prevents both melatonin-induced arachidonic acid and ROS production, whereas LOX inhibition only prevents ROS, indicating that PLA2 is upstream with respect to LOX, as occurs in many signaling pathways. Chlorpromazine, an inhibitor of melatonin-calmodulin interaction, inhibits both ROS and arachidonic acid production, thus possibly placing calmodulin at the origin of a melatonin-induced pro-radical pathway. Interestingly, it is known that Ca 2+-independent PLA2 binds to calmodulin: our results are compatible with PLA2 being liberated by melatonin from a steady-state calmodulin sequestration, thus initiating an arachidonate signal transduction. These results delineate a novel molecular pathway through which melatonin may participate to the inflammatory response.

FEBS Letters, 1995

The possible correlation between DNA digestion and changes in nuclear morphology in apoptosis was... more The possible correlation between DNA digestion and changes in nuclear morphology in apoptosis was studied by blocking the apoptotic process at intermediate stages. The apoptogenic action of three drugs: etoposide, puromycin, tributyltin, was contrasted with protease inhibitors with different specificity on U937 cells. The inhibitors interfered with the development of the apoptotic features without shifting cell death to necrosis: treated cells showed abnormal morphologies, which could be recognized as intermediate stages of apoptosis; accordingly, DNA analysis showed an inhibitor-dependent block of the apoptotic DNA digestion. The comparison between size of DNA fragments and nuclear morphology suggested the following correlations: loss of normal nuclear shape with the appearence of a-2 Mb DNA band; ongoing chromatin condensation with the progressive DNA digestion up to 50 kb; nuclear fragmentation with DNA laddering. Protease inhibitors in etoposide-treated cells did not allow the formation of 700-300 kb fragments, suggesting that they possibly derive from a cell-mediated effect.

Annals of the New York Academy of Sciences, 2003

Mutation Research/Environmental Mutagenesis and Related Subjects, 1985

A nodulação e o crescimento das leguminosas têm influência dos nutrientes contidos no solo, princ... more A nodulação e o crescimento das leguminosas têm influência dos nutrientes contidos no solo, principalmente P, K e Mo. O presente trabalho objetivou avaliar em campo o efeito da inoculação com Bradyrhizobium elkanii e da adubação com P, K e Mo sobre a nodulação, crescimento e produtividade de feijão-caupi (cultivar BRS Guariba). O estudo foi conduzido em uma área experimental, utilizando-se um Neossolo Flúvico, em Teresina, PI. Os tratamentos foram dispostos em um delineamento em blocos casualizados com seis tratamentos e quatro repetições. Os tratamentos consistiram de aplicação ou não de P, K e Mo no solo e de inoculante rizobiano nas sementes. No tratamento com inoculação foi utilizado um inoculante a base de turfa contendo Bradyrhizobium elkanii, estirpe BR 3262. A semeadura foi realizada em parcela experimental de 3,2 m x 5,0 m e procederam-se as coletas dos dados aos 35 e 50 dias após a emergência (DAE) para a determinação do número e massa seca dos nódulos e das massas secas da parte aérea e raiz e acúmulo de N na parte aérea. A produtividade de grãos foi determinada aos 58 DAE. Não se verificou diferença entre os tratamentos para massa seca das raízes, parte aérea e acúmulo de N aos 35 DAE, para massa seca das raízes aos 50 DAE e para a produtividade de grãos. Em solo com adequado conteúdo de P e K a inoculação com Bradyrhizobium elkanii associada à adubação fosfatada, potássica e molíbidica não contribuiu para o aumento do número e massa dos nódulos e nem tampouco da produtividade de grãos de feijão-caupi.

Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 1979

Methyl methanesulphonate (MMS) was used to test the induction of gene mutation, somatic crossing~... more Methyl methanesulphonate (MMS) was used to test the induction of gene mutation, somatic crossing~)ver and mitotic non<lisjunction in A. nidulans. Gene mutation was tested by inducing mutants resistant to 8-azaguanine and revertants of methG1 in a haploid strain. Somatic crossing-over was tested in heterozygous diploids, both with a selective method, i.e. inducing homozygosis to FPA resistance in a heterozygous fpa A1/+ strain, and with a non-selective method, i.e. identifying the frequencies of colour sectors. This latter method was also used to estimate the induction of non<lisjunction because additional markers were present which permitted us to distinguish the two types of colour segregant. Generally, 3 different experimental procedures were used, namely the "plate test", i.e. plating of conidia in agar media containing MMS, and two types of "liquid test", i.e. brief treatment of quiescent or pre-germinated conidia in MMS solution before they were plated on agar media. Point mutations were induced with about equal efficiency with each method, whereas crossing~ver was induced preferentially when germinating conidia were exposed to MMS. On the other hand, non~lisjunction was induced in germinating and quiescent spores with equal efficiency, but such segregants were not recovered with the selective (fpa) method. The results are discussed for both their practical use in the mutagenic testing procedure and their theoretical implication. A. nidulans is, among the lower eukaryotes, the organism in which the greatest variety of genetic damage can be analysed. Work carried out in part under contract No. 177-77-1 ENV I of the E.C. "Environmental Research Programme" and in part under p.f. "Promozione della Qualit~i dell'Ambiente" of the Consiglio Nazionale deHe Ricerche (Italy).

European Journal of Cancer and Clinical Oncology, 1983

Mutation Research/Genetic Toxicology, 1980

Mutation Research/Environmental Mutagenesis and Related Subjects, 1980

Toxicology Letters, 1981

Toxicants of different classes were analysed for capacity to induce gene mutation and mitotic non... more Toxicants of different classes were analysed for capacity to induce gene mutation and mitotic nondisjunction in Aspergillus nidulans, using selective and permissive tests, respectively. Ethanol, Amphotericin B and Micanozole, all affecting membrane integrity, induced only nondisjunction, emphasizing the role of the membrane in mitosis. Benomyl and isopropyl-3-chlorophenyl carbamate (CIPC), two pesticides which interfere with spindle system, induced only non-disjunction. Conversely, Mitomycin C markedly increased mutation rate but not non-disjunction and scarcely affected the viability. The comparative analysis of these two different genetic damages should prove useful in evaluating hazards of drugs.

The Burkitt lymphoma derived Namalwa ceUline is the best studied case of EBV relationship with th... more The Burkitt lymphoma derived Namalwa ceUline is the best studied case of EBV relationship with the human genome. The segments corresponding to tbose adjacent to the inserted viral genome have been cloned from non-infected human cell DNA (1). We bave subc10ned various parts of one of these segments One subclone was used to probe a genomic library from an in vitro EBV immortalized cell line. tbe question being whether hybrid viral­ human clones would be picked up by this probe. In control experiments we tested the same clone on Namalwa DNA where. according to published data and to our confirming sequence and restriction map. it was expected to be linked to tbe rigbt part of the virus. The result did not show linkage as the Namalwa cell line tested in our laboratory and tbe one used by Kieff bad diversified since they separated from the common ancestor. The common origin of the two sub-lines is indisputable on several grounds including our cytogenetic studies. Thus it appears that we a...

Journal of Virology, 1992

We show here that in a lymphoblastoid cell line Epstein-Barr virus DNA recombines with the human ... more We show here that in a lymphoblastoid cell line Epstein-Barr virus DNA recombines with the human genome. The genetic exchange involves the oriP region of the virus. A junction between viral and human DNA from this line has been cloned and sequenced. The results indicate that the integration of Epstein-Barr virus DNA involves a region of the human genome which contains internal short repetition. An 800-bp probe has been isolated from the human part of the junction. This probe has been used to show that the human region exists as a duplication in normal cells.

Epstein-Barr Virus and Human Disease • 1988, 1989

The Burkitt lymphoma derived Namalwa cell line is the best studied case of EBV relationship with ... more The Burkitt lymphoma derived Namalwa cell line is the best studied case of EBV relationship with the human genome. The segments corresponding to those adjacent to the inserted viral genome have been cloned from non-infected human cell DNA (1).

The FASEB Journal, 1999

Static magnetic fields with intensities starting from 6 gauss (6110 04 tesla, T) were found to de... more Static magnetic fields with intensities starting from 6 gauss (6110 04 tesla, T) were found to decrease in an intensity-dependent fashion, reaching a plateau at 6 1 10 03 T, the extent of cell death by apoptosis induced by several agents in different human cell systems. This is not due to a change in the mode of cell death (i.e., to necrosis) or to a delay of the process itself; rather, the presence of magnetic fields allows the indefinite survival and replication of the cells hit by apoptogenic agents. The protective effect was found to be mediated by the ability of the fields to enhance Ca 2/ influx from the extracellular medium; accordingly, it was limited to those cell systems where Ca 2/ influx was shown to have an antiapoptotic effect. Magnetic fields thus might interfere with human health by altering/restoring the equilibrium between cell death and proliferation; indeed, the rescue of damaged cells may be the mechanism explaining why magnetic fields that are not mutagenic per se are often able to increase mutation and tumor frequencies.

Bioorganic & Medicinal Chemistry Letters, 2018

To create your abstract, type over the instructions in the template box below. Fonts or abstract ... more To create your abstract, type over the instructions in the template box below. Fonts or abstract dimensions should not be changed or altered. Oxidative nucleophilic substitution selectively produces cambinol derivatives with antiproliferative activity on bladder cancer cell lines

Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 1987

ABSTRACT The effect of two different mutations, one involving an alpha-tubulin (tubA) and the oth... more ABSTRACT The effect of two different mutations, one involving an alpha-tubulin (tubA) and the other a beta-tubulin (benA33) gene, on somatic segregation has been investigated in diploid strains of A. nidulans. Both mutations, particularly benA33, increase the level of spontaneous chromosomal mis-distribution (CMD) phenomena, without affecting the frequency of crossing-over. The employment of homozygous strains for each of the two mutations in sensitivity tests toward various chemicals, allowed the clear identification of those interfering with microtubule assembly-disassembly processes (i.e. chloral hydrate, diamide, aminocarb, N-ethyl-maleimide, p-chlormercuribenzoate). Such compounds turned out to be very efficient and specific inducers of CMD in a somatic segregation assay performed using the wild-type strain P1. The same assay, when carried out with some of these compounds but employing a tubA/tubA strain, revealed a marked proneness toward CMD to be associated with such mutation, which is known to confer microtubule hypostability.

Mutation Research/Environmental Mutagenesis and Related Subjects, 1978

A simple method capable of detecting recessive lethal damage in a diploid strain of Aspergillus n... more A simple method capable of detecting recessive lethal damage in a diploid strain of Aspergillus nidulans is described. The method scores the recessive lethals on the 1st, the 3rd and the 5th chromosomes, which represent about 40% of the total map of A. nidulans. Two examples of induced lethals, with ultraviolet irradiation and methyl methanesulfonate are shown. The frequency of lethals may reach 36% of the total population with UV irradiation.

Mutation Research/Environmental Mutagenesis and Related Subjects, 1980

Canadian Journal of Genetics and Cytology, 1984

Lines of common wheat cv. 'Chinese Spring' carrying different doses of the Ph1 gene, loca... more Lines of common wheat cv. 'Chinese Spring' carrying different doses of the Ph1 gene, located on the long arm of chromosome 5B were treated with the antimitotic agents griseofulvin and isopropyl-N-phenyl-carbamate (IPC). Treatments with low griseofulvin concentrations and IPC resulted mainly in the production of a high percentage of cells exhibiting spindle disorganization at metaphase and multipolar cells at anaphase–telophase. These treatments did not differentially affect the tested genotypes. On the other hand, higher griseofulvin concentrations induced the appearance of frequent C-metaphases and C-anaphases because of complete disruption of the spindle microtubules; in such cases, more pronounced sensitivity was observed in a line lacking the Ph1 gene than in lines disomic and tetrasomic for chromosome 5B. From this evidence it can be concluded that subcellular structures regulating microtubule organization and orientation, which are apparently the target of IPC and low ...

Mutation Research/Environmental Mutagenesis and Related Subjects, 1979

Quantitative measurement of mitotic non-disjunction with Aspergillus nidulans Methods have been d... more Quantitative measurement of mitotic non-disjunction with Aspergillus nidulans Methods have been developed to test easily non-disjunction with diploid strains of A. nidulans. The methods so far developed are essentially "plate

Handbook of Mutagenicity Test Procedures, 1984

Toxicology and Applied Pharmacology, 2009

We have shown that melatonin immediately and transiently stimulates intracellular free radical pr... more We have shown that melatonin immediately and transiently stimulates intracellular free radical production on a set of leukocytes, possibly as a consequence of calmodulin binding. We show here that melatonininduced ROS are produced by lipoxygenase (LOX), since they are prevented by a set of LOX inhibitors, and are accompanied by increase of the 5-LOX product 5-HETE. LOX activation is accompanied by strong liberation of AA; inhibition of Ca 2+-independent, but not Ca 2+-dependent, phospholipase A2 (PLA2), prevents both melatonin-induced arachidonic acid and ROS production, whereas LOX inhibition only prevents ROS, indicating that PLA2 is upstream with respect to LOX, as occurs in many signaling pathways. Chlorpromazine, an inhibitor of melatonin-calmodulin interaction, inhibits both ROS and arachidonic acid production, thus possibly placing calmodulin at the origin of a melatonin-induced pro-radical pathway. Interestingly, it is known that Ca 2+-independent PLA2 binds to calmodulin: our results are compatible with PLA2 being liberated by melatonin from a steady-state calmodulin sequestration, thus initiating an arachidonate signal transduction. These results delineate a novel molecular pathway through which melatonin may participate to the inflammatory response.

FEBS Letters, 1995

The possible correlation between DNA digestion and changes in nuclear morphology in apoptosis was... more The possible correlation between DNA digestion and changes in nuclear morphology in apoptosis was studied by blocking the apoptotic process at intermediate stages. The apoptogenic action of three drugs: etoposide, puromycin, tributyltin, was contrasted with protease inhibitors with different specificity on U937 cells. The inhibitors interfered with the development of the apoptotic features without shifting cell death to necrosis: treated cells showed abnormal morphologies, which could be recognized as intermediate stages of apoptosis; accordingly, DNA analysis showed an inhibitor-dependent block of the apoptotic DNA digestion. The comparison between size of DNA fragments and nuclear morphology suggested the following correlations: loss of normal nuclear shape with the appearence of a-2 Mb DNA band; ongoing chromatin condensation with the progressive DNA digestion up to 50 kb; nuclear fragmentation with DNA laddering. Protease inhibitors in etoposide-treated cells did not allow the formation of 700-300 kb fragments, suggesting that they possibly derive from a cell-mediated effect.

Annals of the New York Academy of Sciences, 2003

Mutation Research/Environmental Mutagenesis and Related Subjects, 1985

A nodulação e o crescimento das leguminosas têm influência dos nutrientes contidos no solo, princ... more A nodulação e o crescimento das leguminosas têm influência dos nutrientes contidos no solo, principalmente P, K e Mo. O presente trabalho objetivou avaliar em campo o efeito da inoculação com Bradyrhizobium elkanii e da adubação com P, K e Mo sobre a nodulação, crescimento e produtividade de feijão-caupi (cultivar BRS Guariba). O estudo foi conduzido em uma área experimental, utilizando-se um Neossolo Flúvico, em Teresina, PI. Os tratamentos foram dispostos em um delineamento em blocos casualizados com seis tratamentos e quatro repetições. Os tratamentos consistiram de aplicação ou não de P, K e Mo no solo e de inoculante rizobiano nas sementes. No tratamento com inoculação foi utilizado um inoculante a base de turfa contendo Bradyrhizobium elkanii, estirpe BR 3262. A semeadura foi realizada em parcela experimental de 3,2 m x 5,0 m e procederam-se as coletas dos dados aos 35 e 50 dias após a emergência (DAE) para a determinação do número e massa seca dos nódulos e das massas secas da parte aérea e raiz e acúmulo de N na parte aérea. A produtividade de grãos foi determinada aos 58 DAE. Não se verificou diferença entre os tratamentos para massa seca das raízes, parte aérea e acúmulo de N aos 35 DAE, para massa seca das raízes aos 50 DAE e para a produtividade de grãos. Em solo com adequado conteúdo de P e K a inoculação com Bradyrhizobium elkanii associada à adubação fosfatada, potássica e molíbidica não contribuiu para o aumento do número e massa dos nódulos e nem tampouco da produtividade de grãos de feijão-caupi.

Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 1979

Methyl methanesulphonate (MMS) was used to test the induction of gene mutation, somatic crossing~... more Methyl methanesulphonate (MMS) was used to test the induction of gene mutation, somatic crossing~)ver and mitotic non<lisjunction in A. nidulans. Gene mutation was tested by inducing mutants resistant to 8-azaguanine and revertants of methG1 in a haploid strain. Somatic crossing-over was tested in heterozygous diploids, both with a selective method, i.e. inducing homozygosis to FPA resistance in a heterozygous fpa A1/+ strain, and with a non-selective method, i.e. identifying the frequencies of colour sectors. This latter method was also used to estimate the induction of non<lisjunction because additional markers were present which permitted us to distinguish the two types of colour segregant. Generally, 3 different experimental procedures were used, namely the "plate test", i.e. plating of conidia in agar media containing MMS, and two types of "liquid test", i.e. brief treatment of quiescent or pre-germinated conidia in MMS solution before they were plated on agar media. Point mutations were induced with about equal efficiency with each method, whereas crossing~ver was induced preferentially when germinating conidia were exposed to MMS. On the other hand, non~lisjunction was induced in germinating and quiescent spores with equal efficiency, but such segregants were not recovered with the selective (fpa) method. The results are discussed for both their practical use in the mutagenic testing procedure and their theoretical implication. A. nidulans is, among the lower eukaryotes, the organism in which the greatest variety of genetic damage can be analysed. Work carried out in part under contract No. 177-77-1 ENV I of the E.C. "Environmental Research Programme" and in part under p.f. "Promozione della Qualit~i dell'Ambiente" of the Consiglio Nazionale deHe Ricerche (Italy).

European Journal of Cancer and Clinical Oncology, 1983

Mutation Research/Genetic Toxicology, 1980

Mutation Research/Environmental Mutagenesis and Related Subjects, 1980

Toxicology Letters, 1981

Toxicants of different classes were analysed for capacity to induce gene mutation and mitotic non... more Toxicants of different classes were analysed for capacity to induce gene mutation and mitotic nondisjunction in Aspergillus nidulans, using selective and permissive tests, respectively. Ethanol, Amphotericin B and Micanozole, all affecting membrane integrity, induced only nondisjunction, emphasizing the role of the membrane in mitosis. Benomyl and isopropyl-3-chlorophenyl carbamate (CIPC), two pesticides which interfere with spindle system, induced only non-disjunction. Conversely, Mitomycin C markedly increased mutation rate but not non-disjunction and scarcely affected the viability. The comparative analysis of these two different genetic damages should prove useful in evaluating hazards of drugs.

The Burkitt lymphoma derived Namalwa ceUline is the best studied case of EBV relationship with th... more The Burkitt lymphoma derived Namalwa ceUline is the best studied case of EBV relationship with the human genome. The segments corresponding to tbose adjacent to the inserted viral genome have been cloned from non-infected human cell DNA (1). We bave subc10ned various parts of one of these segments One subclone was used to probe a genomic library from an in vitro EBV immortalized cell line. tbe question being whether hybrid viral­ human clones would be picked up by this probe. In control experiments we tested the same clone on Namalwa DNA where. according to published data and to our confirming sequence and restriction map. it was expected to be linked to tbe rigbt part of the virus. The result did not show linkage as the Namalwa cell line tested in our laboratory and tbe one used by Kieff bad diversified since they separated from the common ancestor. The common origin of the two sub-lines is indisputable on several grounds including our cytogenetic studies. Thus it appears that we a...

Journal of Virology, 1992

We show here that in a lymphoblastoid cell line Epstein-Barr virus DNA recombines with the human ... more We show here that in a lymphoblastoid cell line Epstein-Barr virus DNA recombines with the human genome. The genetic exchange involves the oriP region of the virus. A junction between viral and human DNA from this line has been cloned and sequenced. The results indicate that the integration of Epstein-Barr virus DNA involves a region of the human genome which contains internal short repetition. An 800-bp probe has been isolated from the human part of the junction. This probe has been used to show that the human region exists as a duplication in normal cells.

Epstein-Barr Virus and Human Disease • 1988, 1989

The Burkitt lymphoma derived Namalwa cell line is the best studied case of EBV relationship with ... more The Burkitt lymphoma derived Namalwa cell line is the best studied case of EBV relationship with the human genome. The segments corresponding to those adjacent to the inserted viral genome have been cloned from non-infected human cell DNA (1).

The FASEB Journal, 1999

Static magnetic fields with intensities starting from 6 gauss (6110 04 tesla, T) were found to de... more Static magnetic fields with intensities starting from 6 gauss (6110 04 tesla, T) were found to decrease in an intensity-dependent fashion, reaching a plateau at 6 1 10 03 T, the extent of cell death by apoptosis induced by several agents in different human cell systems. This is not due to a change in the mode of cell death (i.e., to necrosis) or to a delay of the process itself; rather, the presence of magnetic fields allows the indefinite survival and replication of the cells hit by apoptogenic agents. The protective effect was found to be mediated by the ability of the fields to enhance Ca 2/ influx from the extracellular medium; accordingly, it was limited to those cell systems where Ca 2/ influx was shown to have an antiapoptotic effect. Magnetic fields thus might interfere with human health by altering/restoring the equilibrium between cell death and proliferation; indeed, the rescue of damaged cells may be the mechanism explaining why magnetic fields that are not mutagenic per se are often able to increase mutation and tumor frequencies.

Bioorganic & Medicinal Chemistry Letters, 2018

To create your abstract, type over the instructions in the template box below. Fonts or abstract ... more To create your abstract, type over the instructions in the template box below. Fonts or abstract dimensions should not be changed or altered. Oxidative nucleophilic substitution selectively produces cambinol derivatives with antiproliferative activity on bladder cancer cell lines

Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 1987

ABSTRACT The effect of two different mutations, one involving an alpha-tubulin (tubA) and the oth... more ABSTRACT The effect of two different mutations, one involving an alpha-tubulin (tubA) and the other a beta-tubulin (benA33) gene, on somatic segregation has been investigated in diploid strains of A. nidulans. Both mutations, particularly benA33, increase the level of spontaneous chromosomal mis-distribution (CMD) phenomena, without affecting the frequency of crossing-over. The employment of homozygous strains for each of the two mutations in sensitivity tests toward various chemicals, allowed the clear identification of those interfering with microtubule assembly-disassembly processes (i.e. chloral hydrate, diamide, aminocarb, N-ethyl-maleimide, p-chlormercuribenzoate). Such compounds turned out to be very efficient and specific inducers of CMD in a somatic segregation assay performed using the wild-type strain P1. The same assay, when carried out with some of these compounds but employing a tubA/tubA strain, revealed a marked proneness toward CMD to be associated with such mutation, which is known to confer microtubule hypostability.

Mutation Research/Environmental Mutagenesis and Related Subjects, 1978

A simple method capable of detecting recessive lethal damage in a diploid strain of Aspergillus n... more A simple method capable of detecting recessive lethal damage in a diploid strain of Aspergillus nidulans is described. The method scores the recessive lethals on the 1st, the 3rd and the 5th chromosomes, which represent about 40% of the total map of A. nidulans. Two examples of induced lethals, with ultraviolet irradiation and methyl methanesulfonate are shown. The frequency of lethals may reach 36% of the total population with UV irradiation.

Mutation Research/Environmental Mutagenesis and Related Subjects, 1980

Canadian Journal of Genetics and Cytology, 1984

Lines of common wheat cv. 'Chinese Spring' carrying different doses of the Ph1 gene, loca... more Lines of common wheat cv. 'Chinese Spring' carrying different doses of the Ph1 gene, located on the long arm of chromosome 5B were treated with the antimitotic agents griseofulvin and isopropyl-N-phenyl-carbamate (IPC). Treatments with low griseofulvin concentrations and IPC resulted mainly in the production of a high percentage of cells exhibiting spindle disorganization at metaphase and multipolar cells at anaphase–telophase. These treatments did not differentially affect the tested genotypes. On the other hand, higher griseofulvin concentrations induced the appearance of frequent C-metaphases and C-anaphases because of complete disruption of the spindle microtubules; in such cases, more pronounced sensitivity was observed in a line lacking the Ph1 gene than in lines disomic and tetrasomic for chromosome 5B. From this evidence it can be concluded that subcellular structures regulating microtubule organization and orientation, which are apparently the target of IPC and low ...

Mutation Research/Environmental Mutagenesis and Related Subjects, 1979

Quantitative measurement of mitotic non-disjunction with Aspergillus nidulans Methods have been d... more Quantitative measurement of mitotic non-disjunction with Aspergillus nidulans Methods have been developed to test easily non-disjunction with diploid strains of A. nidulans. The methods so far developed are essentially "plate

Handbook of Mutagenicity Test Procedures, 1984