Gino Naclerio - Academia.edu (original) (raw)

Papers by Gino Naclerio

Hydrogeology Journal, Mar 25, 2017

Waterborne pathogens represent a significant health risk in both developed and developing countri... more Waterborne pathogens represent a significant health risk in both developed and developing countries with sensitive sub-populations including children, the elderly, neonates, and immune-compromised people, who are particularly susceptible to enteric infections. Annually, approximately 1.8 billion people utilize a faecally contaminated water source, and waterborne diseases are resulting in up to 2.1 million human mortalities globally. Although groundwater has traditionally been considered less susceptible to contamination by enteric pathogens than surface water due to natural attenuation by overlying strata, the degree of microbial removal attributable to soils and aquifers can vary significantly depending on several factors. Thus, accurate assessment of the variable presence and concentration of microbial contaminants, and the relative importance of potentially causative factors affecting contaminant ingress, is critical in order to develop effective source (well) and resource (aquifer) protection strategies. BTraditional^and molecular microbiological study designs, when coupled with hydrogeological, hydrochemical, isotopic, and geophysical methods, have proven useful for analysis of numerous aspects of subsurface microbial dynamics. Accordingly, this overview paper presents the principal microbial techniques currently being employed (1) to predict and identify sources of faecal contamination in groundwater, (2) to elucidate the dynamics of contaminant migration, and (3) to refine knowledge about the hydrogeological characteristics and behaviours of aquifer systems affected by microbial contamination with an emphasis on carbonate aquifers, which represent an important global water supply. Previous investigations carried out in carbonate aquifers in southern Italy are discussed.

Water, Sep 24, 2019

Carbonate aquifers are the primary source of freshwater in Cuba. Unfortunately, coastal groundwat... more Carbonate aquifers are the primary source of freshwater in Cuba. Unfortunately, coastal groundwater is often contaminated by seawater intrusion. The main aim of the present study was to test the efficacy of an experimental modelling approach, ranging from hydrogeology/geomorphology to microbiology, to better characterise both the hydraulic features and behaviour of a coastal carbonate aquifer and acquire useful information to prevent groundwater salinization. The interdisciplinary approach was an effective tool in order to understand (i) the hydraulic role played by some fault zones; (ii) the influence of discontinuous heterogeneities on groundwater flow and saltwater wedge shape; (iii) mixing processes between different water bodies (groundwater, surface water, seawater); (iv) the role of karst conduits in influencing the step-like halocline within the mixing zone between fresh groundwater and seawater.

Environmental Earth Sciences, Jan 20, 2015

ABSTRACT Limestone aquifers provide the main drinking water resources of southern Italy. Due to c... more ABSTRACT Limestone aquifers provide the main drinking water resources of southern Italy. Due to cattle grazing and/or manure spreading, these aquifers are often characterized by microbial contamination of groundwater. The aim of this paper is to summarize the results obtained during a 10-year research carried out in experimental field sites in southern Italy, analyzing (1) the influence of the topsoil of pyroclastic origin on the migration of microbial cells from the ground towards the groundwater, and then on the groundwater vulnerability, (2) the influence of the rainfall regime on the breakthrough at the springs, (3) the reliability of thermotolerant coliforms and fecal enterococci as bacterial indicators of microbial contamination, and (4) the effectiveness of microorganisms as natural tracers for some hydrogeological purposes. The results obtained showed that fecal enterococci are a more reliable indicator than thermotolerant coliforms for detecting contamination and that the entity and distribution over time of microbial contamination of fecal origin are influenced by several factors, such as precipitation regime, thermal regime and existence and thickness of the topsoil of pyroclastic origin. Moreover, the migration of a significant amount of bacterial cells through the topsoil and the underlying carbonate rocks allows the utilization of microorganisms as effective natural tracers, to be coupled with other classic tracers to study the recharge and the flow processes. In a broader perspective, these results can be used to optimize the investigations in other hydrogeological scenarios, with emphasis on those where different water types coexist and interact in same aquifer systems.

Annals of Microbiology, 2020

Purpose Snow and ice ecosystems present unexpectedly high microbial abundance and diversity. Alth... more Purpose Snow and ice ecosystems present unexpectedly high microbial abundance and diversity. Although arctic and alpine snow environments have been intensively investigated from a microbiological point of view, few studies have been conducted in the Apennines. Accordingly, the main purpose of this research was to analyze the microbial communities of the snow collected in two different locations of Capracotta municipality (Southern Italy) after a snowfall record occurred on March 2015 (256 cm of snow in less than 24 h). Methods Bacterial communities were analyzed by the Next-Generation Sequencing techniques. Furthermore, a specific statistical approach for taxonomic hierarchy data was introduced, both for the assessment of diversity within microbial communities and the comparison between different microbiotas. In general, diversity and similarity indices are more informative when computed at the lowest level of the taxonomic hierarchy, the species level. This is not the case with mic...

The main aim of this study was to analyze the influence of rainwater pH on retention of Escherich... more The main aim of this study was to analyze the influence of rainwater pH on retention of Escherichia coli cells and Bacillus subtilis spores in a pyroclastic soil. Nine column experiments were performed in intact soil blocks. The experiments were carried out using antibiotic resistant strains, to be distinguished from the natural background of soil. Column tests were performed at three different solution pH (1.5, 5.6, 8.0). The pH range was chosen taking also into consideration pH values measured in rainwater samples collected at the site were soil blocks were carved. Some physical and hydraulic properties of soil were analyzed by means of standard laboratory tests. Despite the various experimental conditions, comparable re- sults were obtained in all tests. It was observed that retention of both E. coli cells and B. subtilis spores is independent from rainwater pH. This phenomenon is due to a high buffering capacity of the studied soil that inhibits pH-dependent processes that influ...

Annals of Microbiology, 2020

Purpose Truffles are colonized by a complex microbial community of bacteria, yeasts, and filament... more Purpose Truffles are colonized by a complex microbial community of bacteria, yeasts, and filamentous fungi, whose role has not yet been fully understood. The main purpose of the research was to characterize the bacterial communities associated with Tuber aestivum Vittad. fruiting bodies collected from natural truffle grounds in the Molise region (Southern Italy). Despite it is one of the Italian richest areas of truffles, little is known about truffles in Molise. Methods Six ripe fruiting bodies of Tuber aestivum Vittad. and six soil samples were collected in July 2018 at Villa San Michele in the municipality of Vastogirardi, Molise region. Then, soil and truffle microbial communities were analyzed through 16S rRNA gene sequencing on the Illumina MiSeq platform and bioinformatics analyses. Results Consistently with previous studies, the main phyla retrieved in the investigated ascocarps were Proteobacteria and Actinobacteria, with the genus Bradyrhizobium particularly represented. N...

Molecules, 2021

The chemical composition of essential oils (EOs) from dried and fresh flowers of Lavandula angust... more The chemical composition of essential oils (EOs) from dried and fresh flowers of Lavandula angustifolia L. (lavender), named LA 2019 and LA 2020, respectively, grown in central Italy was analyzed and compared by GC and GC-MS. For both samples, 61 compounds were identified, corresponding to 97.9% and 98.1% of the total essential oils. Explorative data analysis, performed to compare the statistical composition of the samples, resulted in a high level of global similarity (around 93%). The compositions of both samples were characterized by 10 major compounds, with a predominance of Linalool (35.3–36.0%), Borneol (15.6–19.4%) and 1,8-Cineole (11.0–9.0%). The in vitro antibacterial activity assay by disk diffusion tests against Bacillus subtilis PY79 and Escherichia coli DH5α showed inhibition of growth in both indicator strains. In addition, plate counts revealed a bactericidal effect on E. coli, which was particularly noticeable when using oil from the fresh lavender flowers at the hig...

Environmental Science and Pollution Research, 2020

Phytomanagement manipulates the soil-plant system to lower the risk posed by contaminated soils. ... more Phytomanagement manipulates the soil-plant system to lower the risk posed by contaminated soils. In this process, the addition of amendments, such as biochar, and bacteria can improve the fertility of poor contaminated soils and consequently ameliorate plant growth. A number of studies based on the inoculation of soil with microorganisms of the genus Bacillus, previously isolated from contaminated sites, revealed positive effects on soil properties and plant growth. Furthermore, when the Bacillus isolates were used in association with biochar, better results were obtained, as biochar can ameliorate soil properties and serve as habitat for microorganisms. Accordingly, a mesocosm study was setup using a mining technosol amended with biochar and inoculated with an endogenous Bacillus isolate, to evaluate the effect of inoculation on soil properties, metal(loid) immobilization, and Salix viminalis growth. Two inoculation methods were compared: (1) direct inoculation of bacteria (Bacillus sp.) and (2) inoculation using biochar as a carrier. Results showed that the Bacillus isolate modified soil properties and ameliorated plant growth, while having a reduced effect on metal(loid) accumulation. The microbial activity was also stimulated, and the community composition was shifted, more importantly when biochar was used as a carrier. In conclusion, this research revealed an improvement of the plant growth and microbial activity after the addition of the endogenous bacterium to the analyzed former mining soil, with better results recorded when a carrier was used.

Geofluids, 2018

Messinian evaporates are widely distributed in the Mediterranean Sea as outcropping sediments in ... more Messinian evaporates are widely distributed in the Mediterranean Sea as outcropping sediments in small marginal basins and in marine cores. Progressive filling of subbasins led to the formation of complex aquifer systems in different regions where hypersaline and fresh water coexist and interact in different manner. It also generates a significant diversification of groundwater hydrochemical signature and different microbial communities. In the case study, the hydrogeology and hydrochemistry of the whole system are influenced by good hydraulic connection between the shallower pyroclastic horizon and the underlying evaporate-bearing fine-grained Messinian succession. This is demonstrated by the merge of hydrogeological, chemical, isotopic, and microbiological data. No mixing with deep ascending waters has been observed. As shown by geophysical, hydraulic, and microbiological investigations, the hydraulic heterogeneity of the Messinian bedrock, mainly due to karstified evaporitic inte...

Beneficial Microbes, 2017

A well-established rat model of diet-induced metabolic syndrome was used to evaluate the effects ... more A well-established rat model of diet-induced metabolic syndrome was used to evaluate the effects of the oral administration of spores or cells of HU16, a carotenoid-producing strain of Bacillus indicus. Symptoms of metabolic syndrome were induced in 90-days old, male Sprague-Dawley rats maintained for eight weeks on a high-fat diet, as previously reported. Parallel groups of animals under the same diet regimen also received a daily dose of 1×1010cells or spores of B. indicus HU16. Cells of strain HU16 were able to reduce symptoms of metabolic syndrome, plasma markers of inflammation and oxidative markers in plasma and liver to levels similar to those observed in rats under a standard diet. HU16 cells did not affect obesity markers or the accumulation of triglycerides in the liver of treated animals. Denaturing gradient gel electrophoresis analysis showed that the oral administration of HU16 cells did not significantly affect the gut microbiota of high fat-fed rats, suggesting that t...

Hydrogeology Journal, 2017

Waterborne pathogens represent a significant health risk in both developed and developing countri... more Waterborne pathogens represent a significant health risk in both developed and developing countries with sensitive sub-populations including children, the elderly, neonates, and immune-compromised people, who are particularly susceptible to enteric infections. Annually, approximately 1.8 billion people utilize a faecally contaminated water source, and waterborne diseases are resulting in up to 2.1 million human mortalities globally. Although groundwater has traditionally been considered less susceptible to contamination by enteric pathogens than surface water due to natural attenuation by overlying strata, the degree of microbial removal attributable to soils and aquifers can vary significantly depending on several factors. Thus, accurate assessment of the variable presence and concentration of microbial contaminants, and the relative importance of potentially causative factors affecting contaminant ingress, is critical in order to develop effective source (well) and resource (aquifer) protection strategies. BTraditional^and molecular microbiological study designs, when coupled with hydrogeological, hydrochemical, isotopic, and geophysical methods, have proven useful for analysis of numerous aspects of subsurface microbial dynamics. Accordingly, this overview paper presents the principal microbial techniques currently being employed (1) to predict and identify sources of faecal contamination in groundwater, (2) to elucidate the dynamics of contaminant migration, and (3) to refine knowledge about the hydrogeological characteristics and behaviours of aquifer systems affected by microbial contamination with an emphasis on carbonate aquifers, which represent an important global water supply. Previous investigations carried out in carbonate aquifers in southern Italy are discussed.

International Journal of Speleology, 2014

Hydrogeology Journal, Mar 25, 2017

Waterborne pathogens represent a significant health risk in both developed and developing countri... more Waterborne pathogens represent a significant health risk in both developed and developing countries with sensitive sub-populations including children, the elderly, neonates, and immune-compromised people, who are particularly susceptible to enteric infections. Annually, approximately 1.8 billion people utilize a faecally contaminated water source, and waterborne diseases are resulting in up to 2.1 million human mortalities globally. Although groundwater has traditionally been considered less susceptible to contamination by enteric pathogens than surface water due to natural attenuation by overlying strata, the degree of microbial removal attributable to soils and aquifers can vary significantly depending on several factors. Thus, accurate assessment of the variable presence and concentration of microbial contaminants, and the relative importance of potentially causative factors affecting contaminant ingress, is critical in order to develop effective source (well) and resource (aquifer) protection strategies. BTraditional^and molecular microbiological study designs, when coupled with hydrogeological, hydrochemical, isotopic, and geophysical methods, have proven useful for analysis of numerous aspects of subsurface microbial dynamics. Accordingly, this overview paper presents the principal microbial techniques currently being employed (1) to predict and identify sources of faecal contamination in groundwater, (2) to elucidate the dynamics of contaminant migration, and (3) to refine knowledge about the hydrogeological characteristics and behaviours of aquifer systems affected by microbial contamination with an emphasis on carbonate aquifers, which represent an important global water supply. Previous investigations carried out in carbonate aquifers in southern Italy are discussed.

Water, Sep 24, 2019

Carbonate aquifers are the primary source of freshwater in Cuba. Unfortunately, coastal groundwat... more Carbonate aquifers are the primary source of freshwater in Cuba. Unfortunately, coastal groundwater is often contaminated by seawater intrusion. The main aim of the present study was to test the efficacy of an experimental modelling approach, ranging from hydrogeology/geomorphology to microbiology, to better characterise both the hydraulic features and behaviour of a coastal carbonate aquifer and acquire useful information to prevent groundwater salinization. The interdisciplinary approach was an effective tool in order to understand (i) the hydraulic role played by some fault zones; (ii) the influence of discontinuous heterogeneities on groundwater flow and saltwater wedge shape; (iii) mixing processes between different water bodies (groundwater, surface water, seawater); (iv) the role of karst conduits in influencing the step-like halocline within the mixing zone between fresh groundwater and seawater.

Environmental Earth Sciences, Jan 20, 2015

ABSTRACT Limestone aquifers provide the main drinking water resources of southern Italy. Due to c... more ABSTRACT Limestone aquifers provide the main drinking water resources of southern Italy. Due to cattle grazing and/or manure spreading, these aquifers are often characterized by microbial contamination of groundwater. The aim of this paper is to summarize the results obtained during a 10-year research carried out in experimental field sites in southern Italy, analyzing (1) the influence of the topsoil of pyroclastic origin on the migration of microbial cells from the ground towards the groundwater, and then on the groundwater vulnerability, (2) the influence of the rainfall regime on the breakthrough at the springs, (3) the reliability of thermotolerant coliforms and fecal enterococci as bacterial indicators of microbial contamination, and (4) the effectiveness of microorganisms as natural tracers for some hydrogeological purposes. The results obtained showed that fecal enterococci are a more reliable indicator than thermotolerant coliforms for detecting contamination and that the entity and distribution over time of microbial contamination of fecal origin are influenced by several factors, such as precipitation regime, thermal regime and existence and thickness of the topsoil of pyroclastic origin. Moreover, the migration of a significant amount of bacterial cells through the topsoil and the underlying carbonate rocks allows the utilization of microorganisms as effective natural tracers, to be coupled with other classic tracers to study the recharge and the flow processes. In a broader perspective, these results can be used to optimize the investigations in other hydrogeological scenarios, with emphasis on those where different water types coexist and interact in same aquifer systems.

Annals of Microbiology, 2020

Purpose Snow and ice ecosystems present unexpectedly high microbial abundance and diversity. Alth... more Purpose Snow and ice ecosystems present unexpectedly high microbial abundance and diversity. Although arctic and alpine snow environments have been intensively investigated from a microbiological point of view, few studies have been conducted in the Apennines. Accordingly, the main purpose of this research was to analyze the microbial communities of the snow collected in two different locations of Capracotta municipality (Southern Italy) after a snowfall record occurred on March 2015 (256 cm of snow in less than 24 h). Methods Bacterial communities were analyzed by the Next-Generation Sequencing techniques. Furthermore, a specific statistical approach for taxonomic hierarchy data was introduced, both for the assessment of diversity within microbial communities and the comparison between different microbiotas. In general, diversity and similarity indices are more informative when computed at the lowest level of the taxonomic hierarchy, the species level. This is not the case with mic...

The main aim of this study was to analyze the influence of rainwater pH on retention of Escherich... more The main aim of this study was to analyze the influence of rainwater pH on retention of Escherichia coli cells and Bacillus subtilis spores in a pyroclastic soil. Nine column experiments were performed in intact soil blocks. The experiments were carried out using antibiotic resistant strains, to be distinguished from the natural background of soil. Column tests were performed at three different solution pH (1.5, 5.6, 8.0). The pH range was chosen taking also into consideration pH values measured in rainwater samples collected at the site were soil blocks were carved. Some physical and hydraulic properties of soil were analyzed by means of standard laboratory tests. Despite the various experimental conditions, comparable re- sults were obtained in all tests. It was observed that retention of both E. coli cells and B. subtilis spores is independent from rainwater pH. This phenomenon is due to a high buffering capacity of the studied soil that inhibits pH-dependent processes that influ...

Annals of Microbiology, 2020

Purpose Truffles are colonized by a complex microbial community of bacteria, yeasts, and filament... more Purpose Truffles are colonized by a complex microbial community of bacteria, yeasts, and filamentous fungi, whose role has not yet been fully understood. The main purpose of the research was to characterize the bacterial communities associated with Tuber aestivum Vittad. fruiting bodies collected from natural truffle grounds in the Molise region (Southern Italy). Despite it is one of the Italian richest areas of truffles, little is known about truffles in Molise. Methods Six ripe fruiting bodies of Tuber aestivum Vittad. and six soil samples were collected in July 2018 at Villa San Michele in the municipality of Vastogirardi, Molise region. Then, soil and truffle microbial communities were analyzed through 16S rRNA gene sequencing on the Illumina MiSeq platform and bioinformatics analyses. Results Consistently with previous studies, the main phyla retrieved in the investigated ascocarps were Proteobacteria and Actinobacteria, with the genus Bradyrhizobium particularly represented. N...

Molecules, 2021

The chemical composition of essential oils (EOs) from dried and fresh flowers of Lavandula angust... more The chemical composition of essential oils (EOs) from dried and fresh flowers of Lavandula angustifolia L. (lavender), named LA 2019 and LA 2020, respectively, grown in central Italy was analyzed and compared by GC and GC-MS. For both samples, 61 compounds were identified, corresponding to 97.9% and 98.1% of the total essential oils. Explorative data analysis, performed to compare the statistical composition of the samples, resulted in a high level of global similarity (around 93%). The compositions of both samples were characterized by 10 major compounds, with a predominance of Linalool (35.3–36.0%), Borneol (15.6–19.4%) and 1,8-Cineole (11.0–9.0%). The in vitro antibacterial activity assay by disk diffusion tests against Bacillus subtilis PY79 and Escherichia coli DH5α showed inhibition of growth in both indicator strains. In addition, plate counts revealed a bactericidal effect on E. coli, which was particularly noticeable when using oil from the fresh lavender flowers at the hig...

Environmental Science and Pollution Research, 2020

Phytomanagement manipulates the soil-plant system to lower the risk posed by contaminated soils. ... more Phytomanagement manipulates the soil-plant system to lower the risk posed by contaminated soils. In this process, the addition of amendments, such as biochar, and bacteria can improve the fertility of poor contaminated soils and consequently ameliorate plant growth. A number of studies based on the inoculation of soil with microorganisms of the genus Bacillus, previously isolated from contaminated sites, revealed positive effects on soil properties and plant growth. Furthermore, when the Bacillus isolates were used in association with biochar, better results were obtained, as biochar can ameliorate soil properties and serve as habitat for microorganisms. Accordingly, a mesocosm study was setup using a mining technosol amended with biochar and inoculated with an endogenous Bacillus isolate, to evaluate the effect of inoculation on soil properties, metal(loid) immobilization, and Salix viminalis growth. Two inoculation methods were compared: (1) direct inoculation of bacteria (Bacillus sp.) and (2) inoculation using biochar as a carrier. Results showed that the Bacillus isolate modified soil properties and ameliorated plant growth, while having a reduced effect on metal(loid) accumulation. The microbial activity was also stimulated, and the community composition was shifted, more importantly when biochar was used as a carrier. In conclusion, this research revealed an improvement of the plant growth and microbial activity after the addition of the endogenous bacterium to the analyzed former mining soil, with better results recorded when a carrier was used.

Geofluids, 2018

Messinian evaporates are widely distributed in the Mediterranean Sea as outcropping sediments in ... more Messinian evaporates are widely distributed in the Mediterranean Sea as outcropping sediments in small marginal basins and in marine cores. Progressive filling of subbasins led to the formation of complex aquifer systems in different regions where hypersaline and fresh water coexist and interact in different manner. It also generates a significant diversification of groundwater hydrochemical signature and different microbial communities. In the case study, the hydrogeology and hydrochemistry of the whole system are influenced by good hydraulic connection between the shallower pyroclastic horizon and the underlying evaporate-bearing fine-grained Messinian succession. This is demonstrated by the merge of hydrogeological, chemical, isotopic, and microbiological data. No mixing with deep ascending waters has been observed. As shown by geophysical, hydraulic, and microbiological investigations, the hydraulic heterogeneity of the Messinian bedrock, mainly due to karstified evaporitic inte...

Beneficial Microbes, 2017

A well-established rat model of diet-induced metabolic syndrome was used to evaluate the effects ... more A well-established rat model of diet-induced metabolic syndrome was used to evaluate the effects of the oral administration of spores or cells of HU16, a carotenoid-producing strain of Bacillus indicus. Symptoms of metabolic syndrome were induced in 90-days old, male Sprague-Dawley rats maintained for eight weeks on a high-fat diet, as previously reported. Parallel groups of animals under the same diet regimen also received a daily dose of 1×1010cells or spores of B. indicus HU16. Cells of strain HU16 were able to reduce symptoms of metabolic syndrome, plasma markers of inflammation and oxidative markers in plasma and liver to levels similar to those observed in rats under a standard diet. HU16 cells did not affect obesity markers or the accumulation of triglycerides in the liver of treated animals. Denaturing gradient gel electrophoresis analysis showed that the oral administration of HU16 cells did not significantly affect the gut microbiota of high fat-fed rats, suggesting that t...

Hydrogeology Journal, 2017

Waterborne pathogens represent a significant health risk in both developed and developing countri... more Waterborne pathogens represent a significant health risk in both developed and developing countries with sensitive sub-populations including children, the elderly, neonates, and immune-compromised people, who are particularly susceptible to enteric infections. Annually, approximately 1.8 billion people utilize a faecally contaminated water source, and waterborne diseases are resulting in up to 2.1 million human mortalities globally. Although groundwater has traditionally been considered less susceptible to contamination by enteric pathogens than surface water due to natural attenuation by overlying strata, the degree of microbial removal attributable to soils and aquifers can vary significantly depending on several factors. Thus, accurate assessment of the variable presence and concentration of microbial contaminants, and the relative importance of potentially causative factors affecting contaminant ingress, is critical in order to develop effective source (well) and resource (aquifer) protection strategies. BTraditional^and molecular microbiological study designs, when coupled with hydrogeological, hydrochemical, isotopic, and geophysical methods, have proven useful for analysis of numerous aspects of subsurface microbial dynamics. Accordingly, this overview paper presents the principal microbial techniques currently being employed (1) to predict and identify sources of faecal contamination in groundwater, (2) to elucidate the dynamics of contaminant migration, and (3) to refine knowledge about the hydrogeological characteristics and behaviours of aquifer systems affected by microbial contamination with an emphasis on carbonate aquifers, which represent an important global water supply. Previous investigations carried out in carbonate aquifers in southern Italy are discussed.

International Journal of Speleology, 2014