Giti Emtiazi - Academia.edu (original) (raw)

Papers by Giti Emtiazi

International journal of systematic and evolutionary microbiology, Jan 25, 2017

A novel halophilic archaeon, designated strain WIIAL99T, was isolated from Lake Meyghan, a hypers... more A novel halophilic archaeon, designated strain WIIAL99T, was isolated from Lake Meyghan, a hypersaline lake in Iran. Cells of strain WIIAL99T were non-motile, catalase-positive and oxidase-negative. Strain WIIAL99T required at least 2.5 M NaCl and 0.05 M MgCl2 for growth. Optimal growth was achieved at 3.5 M NaCl and 0.1 M MgCl2. The optimum pH and temperature for growth were pH 7.0 and 37-40 °C; it was able to grow at pH 6.0-8.5 and 20-55 °C. Cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 8 % (w/v). The major polar lipids of strain WIIAL99T were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, disulfated diglycosyl diether and one unidentified glycolipid. The DNA G+C content of strain WIIAL99T was 66.7 mol%. The closest relative was Natronoarchaeum rubrum JCM 17119T with 98.2 % similarity in the orthologous 16S rRNA gene sequence. Analysis of 16S rRNA and rpoB' gene sequences indicated that strain WIIAL99T is a memb...

International journal of systematic and evolutionary microbiology, Jan 25, 2017

A novel halophilic archaeon, designated strain WIIAL99T, was isolated from Lake Meyghan, a hypers... more A novel halophilic archaeon, designated strain WIIAL99T, was isolated from Lake Meyghan, a hypersaline lake in Iran. Cells of strain WIIAL99T were non-motile, catalase-positive and oxidase-negative. Strain WIIAL99T required at least 2.5 M NaCl and 0.05 M MgCl2 for growth. Optimal growth was achieved at 3.5 M NaCl and 0.1 M MgCl2. The optimum pH and temperature for growth were pH 7.0 and 37-40 °C; it was able to grow at pH 6.0-8.5 and 20-55 °C. Cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 8 % (w/v). The major polar lipids of strain WIIAL99T were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, disulfated diglycosyl diether and one unidentified glycolipid. The DNA G+C content of strain WIIAL99T was 66.7 mol%. The closest relative was Natronoarchaeum rubrum JCM 17119T with 98.2 % similarity in the orthologous 16S rRNA gene sequence. Analysis of 16S rRNA and rpoB' gene sequences indicated that strain WIIAL99T is a memb...

Journal of Petroleum & Environmental Biotechnology, 2011

Journals »». A Accounting & Marketing Addiction Research & Therapy Advances in Automobile... more Journals »». A Accounting & Marketing Addiction Research & Therapy Advances in Automobile Engineering Advances in Pharmacoepidemiology & Drug Safety Advances in Robotics & Automation Aeronautics & Aerospace ...

Current microbiology, Jan 16, 2016

Coal is the most abundant fossil fuel containing sulfur and other elements which promote environm... more Coal is the most abundant fossil fuel containing sulfur and other elements which promote environmental pollution after burning. Also the silicon impurities make the transportation of coal expensive. In this research, two isolated fungi from oil contaminated soil with accessory number KF554100 (Fusarium oxysporum FE) and KC925672 (Exophiala spinifera FM) were used for heterotrophic biological leaching of coal. The leaching were detected by FTIR, CHNS, XRF analyzer and compared with iron and sulfate released in the supernatant. The results showed that E. spinifera FM produced more acidic metabolites in growing cells, promoting the iron and sulfate ions removal while resting cells of F. oxysporum FE enhanced the removal of aromatic sulfur. XRF analysis showed that the resting cells of E. spinifera FM proceeded maximum leaching for iron and silicon (48.8, 43.2 %, respectively). CHNS analysis demonstrated that 34.21 % of sulfur leaching was due to the activities of resting cells of F. ox...

Biotechnology Letters, 2016

To improve the production and activity of an alkaline zinc metalloprotease from Salinivibrio prot... more To improve the production and activity of an alkaline zinc metalloprotease from Salinivibrio proteolyticus in response to ZnSO4 (ionic and nanoparticle forms) and low intensity direct electric current (LIDC). A DC of 50 µA for 10 min increased enzyme production from 35 to 53 U ml(-1) when applied to the stationary phase bacterial cells. Zn(2+) improved enzyme production better than zinc nanoparticles (52 vs. 43.5 U ml(-1)). Zinc nanoparticles (0.5 mM) added to an enzyme reaction mixture containing casein (0.65 %) and 20 mM Tris/HCl buffer (pH 8) improved enzyme activity more than Zn(2+) (42 vs. 36 U ml(-1)). LIDC exposure (50 µA, 10 min) to the stationary phase bacterial cells increases metalloprotease production in Salinivibrio. A low concentration of zinc nanoparticles (0.5 mM) increases maximum enzyme activity.

Process Biochemistry, Jan 1, 2006

Bacteriocin-producing lactic acid bacteria were isolated from 34 samples of dairy products. Nine ... more Bacteriocin-producing lactic acid bacteria were isolated from 34 samples of dairy products. Nine
bacteriocin producers were phenotypically and genotypically identified asEnterococcus faecium.By
means of PCR-techniques, enterocin A was characterised in all of the nine bacteriocin-producing
Enterococcusisolates. Enterocin-producing lactic acid bacteria were the most abundant in dairy
products collected from different areas in Iran. Maximum bacteriocin production by Enterococcus
faecium strains was detected in the stationary phase of growth. Bacteriocins produced by all isolates
were found to have anti-listerial activity in sterile milk. The purified bacteriocins were identified as
<6.5 kDa peptide by sodium dodecyl sulphate-polyacrylamide gel (SDS-PAGE). The molecular
weights of bacteriocins were found to be the same in all strains. This bacteriocin might be useful as
a natural preservative.

International journal of systematic and evolutionary microbiology, Jan 25, 2017

A novel halophilic archaeon, designated strain WIIAL99T, was isolated from Lake Meyghan, a hypers... more A novel halophilic archaeon, designated strain WIIAL99T, was isolated from Lake Meyghan, a hypersaline lake in Iran. Cells of strain WIIAL99T were non-motile, catalase-positive and oxidase-negative. Strain WIIAL99T required at least 2.5 M NaCl and 0.05 M MgCl2 for growth. Optimal growth was achieved at 3.5 M NaCl and 0.1 M MgCl2. The optimum pH and temperature for growth were pH 7.0 and 37-40 °C; it was able to grow at pH 6.0-8.5 and 20-55 °C. Cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 8 % (w/v). The major polar lipids of strain WIIAL99T were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, disulfated diglycosyl diether and one unidentified glycolipid. The DNA G+C content of strain WIIAL99T was 66.7 mol%. The closest relative was Natronoarchaeum rubrum JCM 17119T with 98.2 % similarity in the orthologous 16S rRNA gene sequence. Analysis of 16S rRNA and rpoB' gene sequences indicated that strain WIIAL99T is a memb...

International journal of systematic and evolutionary microbiology, Jan 25, 2017

A novel halophilic archaeon, designated strain WIIAL99T, was isolated from Lake Meyghan, a hypers... more A novel halophilic archaeon, designated strain WIIAL99T, was isolated from Lake Meyghan, a hypersaline lake in Iran. Cells of strain WIIAL99T were non-motile, catalase-positive and oxidase-negative. Strain WIIAL99T required at least 2.5 M NaCl and 0.05 M MgCl2 for growth. Optimal growth was achieved at 3.5 M NaCl and 0.1 M MgCl2. The optimum pH and temperature for growth were pH 7.0 and 37-40 °C; it was able to grow at pH 6.0-8.5 and 20-55 °C. Cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 8 % (w/v). The major polar lipids of strain WIIAL99T were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, disulfated diglycosyl diether and one unidentified glycolipid. The DNA G+C content of strain WIIAL99T was 66.7 mol%. The closest relative was Natronoarchaeum rubrum JCM 17119T with 98.2 % similarity in the orthologous 16S rRNA gene sequence. Analysis of 16S rRNA and rpoB' gene sequences indicated that strain WIIAL99T is a memb...

Journal of Petroleum & Environmental Biotechnology, 2011

Journals »». A Accounting & Marketing Addiction Research & Therapy Advances in Automobile... more Journals »». A Accounting & Marketing Addiction Research & Therapy Advances in Automobile Engineering Advances in Pharmacoepidemiology & Drug Safety Advances in Robotics & Automation Aeronautics & Aerospace ...

Current microbiology, Jan 16, 2016

Coal is the most abundant fossil fuel containing sulfur and other elements which promote environm... more Coal is the most abundant fossil fuel containing sulfur and other elements which promote environmental pollution after burning. Also the silicon impurities make the transportation of coal expensive. In this research, two isolated fungi from oil contaminated soil with accessory number KF554100 (Fusarium oxysporum FE) and KC925672 (Exophiala spinifera FM) were used for heterotrophic biological leaching of coal. The leaching were detected by FTIR, CHNS, XRF analyzer and compared with iron and sulfate released in the supernatant. The results showed that E. spinifera FM produced more acidic metabolites in growing cells, promoting the iron and sulfate ions removal while resting cells of F. oxysporum FE enhanced the removal of aromatic sulfur. XRF analysis showed that the resting cells of E. spinifera FM proceeded maximum leaching for iron and silicon (48.8, 43.2 %, respectively). CHNS analysis demonstrated that 34.21 % of sulfur leaching was due to the activities of resting cells of F. ox...

Biotechnology Letters, 2016

To improve the production and activity of an alkaline zinc metalloprotease from Salinivibrio prot... more To improve the production and activity of an alkaline zinc metalloprotease from Salinivibrio proteolyticus in response to ZnSO4 (ionic and nanoparticle forms) and low intensity direct electric current (LIDC). A DC of 50 µA for 10 min increased enzyme production from 35 to 53 U ml(-1) when applied to the stationary phase bacterial cells. Zn(2+) improved enzyme production better than zinc nanoparticles (52 vs. 43.5 U ml(-1)). Zinc nanoparticles (0.5 mM) added to an enzyme reaction mixture containing casein (0.65 %) and 20 mM Tris/HCl buffer (pH 8) improved enzyme activity more than Zn(2+) (42 vs. 36 U ml(-1)). LIDC exposure (50 µA, 10 min) to the stationary phase bacterial cells increases metalloprotease production in Salinivibrio. A low concentration of zinc nanoparticles (0.5 mM) increases maximum enzyme activity.

Process Biochemistry, Jan 1, 2006

Bacteriocin-producing lactic acid bacteria were isolated from 34 samples of dairy products. Nine ... more Bacteriocin-producing lactic acid bacteria were isolated from 34 samples of dairy products. Nine
bacteriocin producers were phenotypically and genotypically identified asEnterococcus faecium.By
means of PCR-techniques, enterocin A was characterised in all of the nine bacteriocin-producing
Enterococcusisolates. Enterocin-producing lactic acid bacteria were the most abundant in dairy
products collected from different areas in Iran. Maximum bacteriocin production by Enterococcus
faecium strains was detected in the stationary phase of growth. Bacteriocins produced by all isolates
were found to have anti-listerial activity in sterile milk. The purified bacteriocins were identified as
<6.5 kDa peptide by sodium dodecyl sulphate-polyacrylamide gel (SDS-PAGE). The molecular
weights of bacteriocins were found to be the same in all strains. This bacteriocin might be useful as
a natural preservative.