Giuseppe Palladino - Academia.edu (original) (raw)
Papers by Giuseppe Palladino
Nephrology Dialysis Transplantation, 2012
Introduction and Aims: Advanced Chronic Kidney Disease (CKD) is gaining increasing medical and ec... more Introduction and Aims: Advanced Chronic Kidney Disease (CKD) is gaining increasing medical and economical importance. However there is not enough reliable information about disease course, treatmentvariation, and the impact of treatments under routine clinical conditions on patient survival, quality of life, and costs. This paper demonstrates the first results of a nationwide electronicregistry, based on nephrology clinic data, for the prospective observation of CKD in Germany Methods: A large network of nephrologist practices across Germany (FNDN, Forschungsnetzwerk Verband Deutsche Nierenzentren) is participating in the project. In each participating center, routinely obtained clinical data for non-dialysis dependent CKD patients are documented electronically including age, gender, cause of CKD, comorbidities, laboratory values, hospitalisations, blood pressure, and medications. Data are collected quarterly by a software called QuaNT (Qualitäsicherung Nephrologie und Transplantation). Results: Cross-sectional data are provided from 59 participating nephrology clinics and 6,187 patients with CKD Stage 3-5 in the second quarter of 2011. The mean age ± standard deviation (SD) was 72 ± 12 years. The distribution of CKD Stages 3, 4, and 5 (Non-dialysis) was 60, 33, and 8%, respectively. Diabetic nephropathy was diagnosed in 26% of patients; the major underlying renal disease was hypertension/ vascular nephropathy with 47%. Renin-angiotensin-system (RAS) inhibitors were prescribed in 78% of all patients. Vitamin D prescription (any) was observed in 43%, phosphate binders in 5%, iron (oral or IV) in 19%, erythropoietin-stimulating agents (ESA) in 15%, and aldosterone antagonists (spironolactone) in 8% of patients. Conclusions: This nationwide registry is a new initiative that provides a nationwide electronic data base to prospectively follow clinical care and outcomes among CKD patients. The number of participating patients and practices is expected to increase considerably. Variations in patient care, as typically practiced, will be evaluated to identify best treatment practices in analyses integrated into the international Chronic Kidney Disease Outcomes and Practice Pattern Study (CKDopps).
Bulletin of the Chemical Society of Japan
Advance Publication is a service for online publication of manuscripts prior to releasing fully e... more Advance Publication is a service for online publication of manuscripts prior to releasing fully edited, printed versions. Entire manuscripts and a portion of the graphical abstract can be released on the web as soon as the submission is accepted. Note that the Chemical Society of Japan bears no responsibility for issues resulting from the use of information taken from unedited, Advance Publication manuscripts.
Artificial Organs, 2012
Phenols are uremic toxins of intestinal origin formed by bacteria during protein metabolism. Of t... more Phenols are uremic toxins of intestinal origin formed by bacteria during protein metabolism. Of these molecules, p-cresol is the most studied and has been associated with renal function impairment and vascular damage. Bisphenol A (BPA) is a molecule with structural similarity with phenols found in plastic food and beverage containers as well as in some dialyzers. BPA is considered an environmental toxicant based on animal and cell culture studies. Japanese authorities recently banned BPA use in baby bottles based on observational association studies in newborns. BPA is excreted in urine and uremic patients present higher serum levels, but there is insufficient evidence to set cutoff levels or to link BPA to any harmful effect in CKD. However, the renal elimination and potential exposure during dialysis warrant the monitoring of BPA exposure and the design of observational studies in which the potential health risks of BPA for end-stage renal disease patients are evaluated.
Giornale italiano di nefrologia : organo ufficiale della Societa italiana di nefrologia, Jan 5, 2017
Patients affected by hilar cholangiocarcinoma are eligible for surgery only in the 20-30% of the ... more Patients affected by hilar cholangiocarcinoma are eligible for surgery only in the 20-30% of the cases and postoperative mortality is 40-50%. Many specialists are involved in the treatment of this disease, like surgeons, gastroenterologists, oncologists and radiotherapists. Recent studies have shown that preoperative bilirubinaemia is a predictor of morbidity and mortality after surgery. Coupled Plasma Filtration and Adsorption (CPFA) is a blood purification extracorporeal therapy recommended for sepsis and able to reduce bilirubinaemia. We treated 10 patients referred to our centre affected by hilar cholangiocarcinoma complicated by obstructive jaundice with 34 CPFA sessions to test its ability to reduce preoperative bilirubin levels and we checked for mortality at 90 days. CPFA reduced preoperative bilirubin of 30% for session; it also improved others inflammation and coagulation tests. Mortality at 90 days was 40%. CPFA is an effective therapy for hyperbilirubinaemia. Lowering pr...
BMC Nephrology, 2016
Background: Hemodiafiltration with on-line endogenous reinfusion (HFR) is an extracorporeal dialy... more Background: Hemodiafiltration with on-line endogenous reinfusion (HFR) is an extracorporeal dialytic method that combines diffusion, convection and adsorption. HFR-Supra (HFR-S) is a second-generation system with increased convective permeability and adsorption capability. Previous studies suggested that HFR reduces oxidative stress compared to standard haemodialysis. The principal aim of the present study was to compare antioxidant vitamins behavior and oxidative status of hemodialysis patients treated with HFR and HFR-S. Methods: The study was designed as a multicenter, randomized, crossover trial. Forty-one patients were recruited from 19 dialysis centers and after a 4-month washout stabilization period in on-line hemodiafiltration (ol-HDF), each patient was randomized to a sequence of treatments (HFR-S followed by HFR or viceversa) with each treatment applied over 6 months. Plasma levels of Advanced Oxidation Protein Products, Total Antioxidant Status, vitamins C, A and E and their ligands (Retinol Binding Protein and total lipids) were measured at baseline and at the end of each treatment period. Results: Results show that the higher convective permeability of HFR-S with respect to HFR did not produce additional beneficial effects on the patients' oxidative status, a slight decrease of both Vitamin A and Retinol Binding Protein being the only difference registered in the long-term. However, as compared to ol-HDF, both the re-infusive techniques allowed to reduce the intradialytic loss of Vitamin C and, in the long-term, improve the patients' oxidative status and increase Retinol Binding Protein plasma values. No significant differences were found between the Vitamin C concentration of pre-and post cartridge UF neither in HFR-S nor in HFR showing that the sorbent resin does not adsorb Vitamin C. Conclusion: HFR-S and HFR are almost equivalent in term of impact on antioxidant vitamins and oxidative status of hemodialysis patients. Nonetheless, as compared to ol-HDF, both treatments produced a sensible sparing of Vitamin C and may represent a new approach for reducing oxidative stress and related complications in dialysis patients. Longterm effects of re-infusive treatments on patients' cardiovascular morbidity and mortality need to be evaluated. Trial registration: ClinicalTrials.gov Identifier NCT01492491, retrospectively registered in 10 December 2011.
F1000posters, Jul 18, 2011
Chronic renal failure patients accumulate in the blood molecules that are normally excreted into ... more Chronic renal failure patients accumulate in the blood molecules that are normally excreted into the urine. p-Cresol Sulphate (pCS), the most representative retained toxin, shows a high level of toxicity. Therefore, its quantification could represent a prediction factor to determine the risk of endothelial dysfunction and cardiovascular complication and response to the haemodialysis treatment. The aim of this study was to evaluate the suitability of the multiple reaction monitoring (MRM) technique in order to improve the sensibility, the selectivity and the timing of pCS detection in a small amount of plasma. Deproteinized plasma of uremic patients was concentrated and dissolved in liquid chromatography (LC) mobile phase solution. pCS was quantified by LC coupled to tandem mass spectrometry (LC-MS/MS) on a triple-quadrupole mass spectrometer. Selective and sensitive detection of pCS was achieved by selecting the specific parent ion and monitoring two specific fragment ions. The MRM assay was carried out using the following transitions: m/z 187 → 80.00 and m/z 187 → 107.00. A good linearity was observed for each calibration curve. The intra-day and inter-day results showed a good precision and repeatability. The percentage recoveries indicate an optimal selectivity of the analytical method. The MRM assay to quantify pCS in a small amount of human plasma is rapid, highly sensitive, selective and with a good repeatability.
Acta Crystallogr E Struct Rep, 2005
Biotechnology and Applied Biochemistry, 2016
Bacterial-derived DNA fragments (BDNAs) have been shown to be present in dialysis fluid, to pass ... more Bacterial-derived DNA fragments (BDNAs) have been shown to be present in dialysis fluid, to pass through dialyzer membranes, and to induce IL-6 (IL-6) in mononuclear cells. DNA fragments are thought to be derived from microorganisms inhabiting hemodialysis water and fluid. The primary aim of the present study was to develop two degenerated TAQMAN real time Q-PCR for detection of a broad-range of bacterial DNA which specifically detects 16S rDNA (862 bp, and 241 bp) and evaluate the efficiency of the Bellco Selecta resin to capture the BDNAs in the dialysis fluid. For this purpose, we decided to compare measurements of un-fragmented samples (9.8×10(5) E.coli genome) with artificially fragmented DNA samples.We assessed two broad-range real-time PCR targeting bacterial 16S rRNA genes for detection of fragmented and un-fragmented bacterial DNA in dialytic fluid and demonstrated that Bellco Selecta resin is capable to retain these type of bacterial DNA. This article is protected by copyright. All rights reserved.
Journal of Nephrology, 2014
In myeloma cast nephropathy, fast reduction of serum free light chain (FLC) levels correlates wit... more In myeloma cast nephropathy, fast reduction of serum free light chain (FLC) levels correlates with renal recovery. Recently, extracorporeal treatments using filters with a high-molecular weight cut-off have been successfully used for FLC removal. However, using these new filters, high cost and elevated albumin leakage are common drawbacks. We studied a new and cheaper therapeutic approach with adsorbent resins to evaluate its efficacy. We treated four patients, affected by dialysis-dependent acute kidney injury (AKI) due to biopsy proven de novo FLC myeloma cast nephropathy. Each patient underwent bortezomib chemotherapy and extracorporeal treatment with the supra-hemodiafiltration with endogenous reinfusion (HFR) technique (Supra-HFR, Bellco Mirandola, Modena, Italy). Supra-HFR is a kind of hemodiafiltration that utilizes separated convection, diffusion and adsorption. The sorbent cartridge has a high affinity for FLC (both κ and λ) but is able to re-infuse albumin, avoiding the need for albumin perfusions. Supra HFR treatments (4 h each) were carried out for eight consecutive days and then every other day. All patients showed a significant reduction of serum FLC, whereas serum albumin concentration remained unchanged. Renal function recovered in three out of four patients. FLC removal with adsorbent resins represents an effective therapeutic strategy that does not require replacement with albumin .
Nephrology Dialysis Transplantation, 2014
Introduction and Aims: Online hemodiafiltration (HDF) with high-volume substitution fluid is an o... more Introduction and Aims: Online hemodiafiltration (HDF) with high-volume substitution fluid is an optimal way to remove uremic substances ranging widely in molecular size from small to low molecular weight (MW). Post-dilution HDF is the most efficient infusion mode to obtain maximum clearance.Mid-dilution infusion is an interesting alternative that represents simultaneous pre-and post-dilution infusion modes and could be a highly effective technique to remove uremic toxins, avoiding the disadvantages of pre-and post-dilutional modes.The aim of this multicentric study was to compare mid-dilution (MID) with post-dilution HDF (POST) by evaluating their efficiency in removing different middle MW (MMW) and protein bound uremic toxins. Methods: We performed a cross-over study including 158 Patients from 21 centres. All patients were randomized in two different groups for three months: group A (1 month MID-1 month POST-1 month MID) and group B (1 month POST-1 month MID-1 month POST). 64 patients were excluded from the final analysis because of incomplete data. The reduction rate (RR) of middle and protein bound molecules were centrally determined from serum samples as well as the second generation Daugirdas Kt/Vd. Albumin loss was carried out for both groups.Unpaired t student test was performed using GraphPad prism version 4.00 for Windows. SP411 Baseline patients characteristics and RRF survival by group Age years Weight kg baseline GFR mL/min baseline Kt/V per session deltaGFR ml/min/y mean RRF survival months 2-y cum surv % G1 N=48
Abstract. Making crystals by design is the paradigm of crystal engineering. The main goal is that... more Abstract. Making crystals by design is the paradigm of crystal engineering. The main goal is that of obtaining and controlling the collective properties of a crystalline material from the convolution of the physical and chemical properties of the individual building blocks (whether ...
Journal of Materials Science: Materials in Medicine, 2014
Your article is protected by copyright and all rights are held exclusively by Springer Science +B... more Your article is protected by copyright and all rights are held exclusively by Springer Science +Business Media New York. This e-offprint is for personal use only and shall not be selfarchived in electronic repositories. If you wish to self-archive your article, please use the accepted manuscript version for posting on your own website. You may further deposit the accepted manuscript version in any repository, provided it is only made publicly available 12 months after official publication or later and provided acknowledgement is given to the original source of publication and a link is inserted to the published article on Springer's website. The link must be accompanied by the following text: "The final publication is available at link.springer.com".
Annali di Chimica, 2005
The complex formation between the dioxouranium (VI) and the oxalate ions has been investigated by... more The complex formation between the dioxouranium (VI) and the oxalate ions has been investigated by measuring the potential of a glass electrode, at 25.00 degrees C, in 1 and 3 M NaClO4, at lower acidities than 10(-4.5) M, in order to favour the formation of (mixed) ternary species. The upper limits of concentration of all the analytical parameters have been imposed by the modest solubility of Na2C2O4 in the ionic media. The experimental measurements at different ionic strengths have been treated by means of the computerised least square programme LETAGROP - ETITR. Ternary complexes of general composition (p, q, r) are formed according to reaction (1), in addition to the already reported binary complexes. pUO2(2+) + qH2O + rC2O4(2-) <==> (UO2)p(OH)q(C2O4)r(2p-q-2r)(+) + qH+ (1). The stoichiometric compositions of the ternary species are (1, 1, 1), (2, 4, 2), (2, 2, 4). Their formation constants, expressed in molality, obtained in the two ionic media, are listed below. [table: see text]. For reasons discussed in the present work in the last column only the value of the constant in 1 M ionic medium is reported for the species (2, 4, 2). Additional evidence on the stoichiometric composition of the species formed is afforded by the mass-spectrometric measurements, collected in solutions of known composition.
Nephrology Dialysis Transplantation, 2014
Introduction and Aims: A high level of fibroblast growth factor 23 (FGF23) is a risk factor for m... more Introduction and Aims: A high level of fibroblast growth factor 23 (FGF23) is a risk factor for mortality, and recent studies have linked FGF23 to parameters of volume homeostasis and an increased risk of heart failure. In hemodialysis (HD), a large ultrafiltration volume (UFV) is also associated with an increased mortality risk. We aimed to investigate whether circulating FGF23 levels and ultrafiltration volume are related in a cohort of stable HD patients. Methods: Post-hoc analysis on a prospective cohort study of 104 HD patients, median age 66 (interquartile range 51-75) years, dialysis vintage 25.0 (8.5-51.2) months, who underwent a standard four-hour HD session at the first session of the week. Blood samples were drawn at onset of HD. Plasma C-terminal FGF23 was determined by ELISA. Residual renal function (RRF) and Kt/V were extracted from patient records. We used uni-and multivariate linear regression to assess the association between UFV and FGF23. Natural log (Ln)-transformation was applied when appropriate. Results: At start of the HD session the median FGF23 level was 7535 [interquartile range 3276-13433] RU/mL. Mean UFV throughout the HD session was 2561 (standard deviation ±771) mL. In univariate analysis, natural log-transformed (Ln) FGF23 levels correlated with UFV (Figure 1), and also with serum phosphate (R=0.587, P<0.001), age (R=-0.384 P<0.001), and Ln Kt/V (R=-0.252 P=0.01), but not significantly with calcium (R=0.167 P=0.09). Multivariate linear regression analysis revealed a consistent strong association between Ln FGF23 AND UFV (St. β 0.385, P<0.001), in a model adjusted for serum phosphate (Standardized β 0.451, P<0.001) and serum calcium (St. β 0.222, P=0.002; model R2 52%). Age, gender, dialysis vintage, Kt/V, systolic and diastolic blood pressure did not contribute to this model. The association between FGF23 and UFV was independent of serum phosphate (Figures 1, 2). Patients with relevant residual renal function had a trend for lower FGF23 levels (Mann-Whitney P=0.04, Spearman's Rho-0.230 P=0.04), however RRF was no correlate of FGF23 in multivariate analysis (St. β-0.111, P=0.16).
Thermochimica Acta, 2002
The coordination chemistry of dioxouranium(VI) with organic ligands may represent an useful model... more The coordination chemistry of dioxouranium(VI) with organic ligands may represent an useful model to describe the migration of radionuclides in natural fluids. A new dioxouranium(VI) ternary complex of formula Na4(UO2)2(OH)4(C2O4)2 was precipitated at pH≅8. It was characterized by elemental analysis, ICP and IR spectroscopy, thermogravimetry (TG) and coupled TG–IR evolved gas analysis. Potentiometric measurements in the solution suggest that the
Nephrology Dialysis Transplantation, 2012
Introduction and Aims: Advanced Chronic Kidney Disease (CKD) is gaining increasing medical and ec... more Introduction and Aims: Advanced Chronic Kidney Disease (CKD) is gaining increasing medical and economical importance. However there is not enough reliable information about disease course, treatmentvariation, and the impact of treatments under routine clinical conditions on patient survival, quality of life, and costs. This paper demonstrates the first results of a nationwide electronicregistry, based on nephrology clinic data, for the prospective observation of CKD in Germany Methods: A large network of nephrologist practices across Germany (FNDN, Forschungsnetzwerk Verband Deutsche Nierenzentren) is participating in the project. In each participating center, routinely obtained clinical data for non-dialysis dependent CKD patients are documented electronically including age, gender, cause of CKD, comorbidities, laboratory values, hospitalisations, blood pressure, and medications. Data are collected quarterly by a software called QuaNT (Qualitäsicherung Nephrologie und Transplantation). Results: Cross-sectional data are provided from 59 participating nephrology clinics and 6,187 patients with CKD Stage 3-5 in the second quarter of 2011. The mean age ± standard deviation (SD) was 72 ± 12 years. The distribution of CKD Stages 3, 4, and 5 (Non-dialysis) was 60, 33, and 8%, respectively. Diabetic nephropathy was diagnosed in 26% of patients; the major underlying renal disease was hypertension/ vascular nephropathy with 47%. Renin-angiotensin-system (RAS) inhibitors were prescribed in 78% of all patients. Vitamin D prescription (any) was observed in 43%, phosphate binders in 5%, iron (oral or IV) in 19%, erythropoietin-stimulating agents (ESA) in 15%, and aldosterone antagonists (spironolactone) in 8% of patients. Conclusions: This nationwide registry is a new initiative that provides a nationwide electronic data base to prospectively follow clinical care and outcomes among CKD patients. The number of participating patients and practices is expected to increase considerably. Variations in patient care, as typically practiced, will be evaluated to identify best treatment practices in analyses integrated into the international Chronic Kidney Disease Outcomes and Practice Pattern Study (CKDopps).
Bulletin of the Chemical Society of Japan
Advance Publication is a service for online publication of manuscripts prior to releasing fully e... more Advance Publication is a service for online publication of manuscripts prior to releasing fully edited, printed versions. Entire manuscripts and a portion of the graphical abstract can be released on the web as soon as the submission is accepted. Note that the Chemical Society of Japan bears no responsibility for issues resulting from the use of information taken from unedited, Advance Publication manuscripts.
Artificial Organs, 2012
Phenols are uremic toxins of intestinal origin formed by bacteria during protein metabolism. Of t... more Phenols are uremic toxins of intestinal origin formed by bacteria during protein metabolism. Of these molecules, p-cresol is the most studied and has been associated with renal function impairment and vascular damage. Bisphenol A (BPA) is a molecule with structural similarity with phenols found in plastic food and beverage containers as well as in some dialyzers. BPA is considered an environmental toxicant based on animal and cell culture studies. Japanese authorities recently banned BPA use in baby bottles based on observational association studies in newborns. BPA is excreted in urine and uremic patients present higher serum levels, but there is insufficient evidence to set cutoff levels or to link BPA to any harmful effect in CKD. However, the renal elimination and potential exposure during dialysis warrant the monitoring of BPA exposure and the design of observational studies in which the potential health risks of BPA for end-stage renal disease patients are evaluated.
Giornale italiano di nefrologia : organo ufficiale della Societa italiana di nefrologia, Jan 5, 2017
Patients affected by hilar cholangiocarcinoma are eligible for surgery only in the 20-30% of the ... more Patients affected by hilar cholangiocarcinoma are eligible for surgery only in the 20-30% of the cases and postoperative mortality is 40-50%. Many specialists are involved in the treatment of this disease, like surgeons, gastroenterologists, oncologists and radiotherapists. Recent studies have shown that preoperative bilirubinaemia is a predictor of morbidity and mortality after surgery. Coupled Plasma Filtration and Adsorption (CPFA) is a blood purification extracorporeal therapy recommended for sepsis and able to reduce bilirubinaemia. We treated 10 patients referred to our centre affected by hilar cholangiocarcinoma complicated by obstructive jaundice with 34 CPFA sessions to test its ability to reduce preoperative bilirubin levels and we checked for mortality at 90 days. CPFA reduced preoperative bilirubin of 30% for session; it also improved others inflammation and coagulation tests. Mortality at 90 days was 40%. CPFA is an effective therapy for hyperbilirubinaemia. Lowering pr...
BMC Nephrology, 2016
Background: Hemodiafiltration with on-line endogenous reinfusion (HFR) is an extracorporeal dialy... more Background: Hemodiafiltration with on-line endogenous reinfusion (HFR) is an extracorporeal dialytic method that combines diffusion, convection and adsorption. HFR-Supra (HFR-S) is a second-generation system with increased convective permeability and adsorption capability. Previous studies suggested that HFR reduces oxidative stress compared to standard haemodialysis. The principal aim of the present study was to compare antioxidant vitamins behavior and oxidative status of hemodialysis patients treated with HFR and HFR-S. Methods: The study was designed as a multicenter, randomized, crossover trial. Forty-one patients were recruited from 19 dialysis centers and after a 4-month washout stabilization period in on-line hemodiafiltration (ol-HDF), each patient was randomized to a sequence of treatments (HFR-S followed by HFR or viceversa) with each treatment applied over 6 months. Plasma levels of Advanced Oxidation Protein Products, Total Antioxidant Status, vitamins C, A and E and their ligands (Retinol Binding Protein and total lipids) were measured at baseline and at the end of each treatment period. Results: Results show that the higher convective permeability of HFR-S with respect to HFR did not produce additional beneficial effects on the patients' oxidative status, a slight decrease of both Vitamin A and Retinol Binding Protein being the only difference registered in the long-term. However, as compared to ol-HDF, both the re-infusive techniques allowed to reduce the intradialytic loss of Vitamin C and, in the long-term, improve the patients' oxidative status and increase Retinol Binding Protein plasma values. No significant differences were found between the Vitamin C concentration of pre-and post cartridge UF neither in HFR-S nor in HFR showing that the sorbent resin does not adsorb Vitamin C. Conclusion: HFR-S and HFR are almost equivalent in term of impact on antioxidant vitamins and oxidative status of hemodialysis patients. Nonetheless, as compared to ol-HDF, both treatments produced a sensible sparing of Vitamin C and may represent a new approach for reducing oxidative stress and related complications in dialysis patients. Longterm effects of re-infusive treatments on patients' cardiovascular morbidity and mortality need to be evaluated. Trial registration: ClinicalTrials.gov Identifier NCT01492491, retrospectively registered in 10 December 2011.
F1000posters, Jul 18, 2011
Chronic renal failure patients accumulate in the blood molecules that are normally excreted into ... more Chronic renal failure patients accumulate in the blood molecules that are normally excreted into the urine. p-Cresol Sulphate (pCS), the most representative retained toxin, shows a high level of toxicity. Therefore, its quantification could represent a prediction factor to determine the risk of endothelial dysfunction and cardiovascular complication and response to the haemodialysis treatment. The aim of this study was to evaluate the suitability of the multiple reaction monitoring (MRM) technique in order to improve the sensibility, the selectivity and the timing of pCS detection in a small amount of plasma. Deproteinized plasma of uremic patients was concentrated and dissolved in liquid chromatography (LC) mobile phase solution. pCS was quantified by LC coupled to tandem mass spectrometry (LC-MS/MS) on a triple-quadrupole mass spectrometer. Selective and sensitive detection of pCS was achieved by selecting the specific parent ion and monitoring two specific fragment ions. The MRM assay was carried out using the following transitions: m/z 187 → 80.00 and m/z 187 → 107.00. A good linearity was observed for each calibration curve. The intra-day and inter-day results showed a good precision and repeatability. The percentage recoveries indicate an optimal selectivity of the analytical method. The MRM assay to quantify pCS in a small amount of human plasma is rapid, highly sensitive, selective and with a good repeatability.
Acta Crystallogr E Struct Rep, 2005
Biotechnology and Applied Biochemistry, 2016
Bacterial-derived DNA fragments (BDNAs) have been shown to be present in dialysis fluid, to pass ... more Bacterial-derived DNA fragments (BDNAs) have been shown to be present in dialysis fluid, to pass through dialyzer membranes, and to induce IL-6 (IL-6) in mononuclear cells. DNA fragments are thought to be derived from microorganisms inhabiting hemodialysis water and fluid. The primary aim of the present study was to develop two degenerated TAQMAN real time Q-PCR for detection of a broad-range of bacterial DNA which specifically detects 16S rDNA (862 bp, and 241 bp) and evaluate the efficiency of the Bellco Selecta resin to capture the BDNAs in the dialysis fluid. For this purpose, we decided to compare measurements of un-fragmented samples (9.8×10(5) E.coli genome) with artificially fragmented DNA samples.We assessed two broad-range real-time PCR targeting bacterial 16S rRNA genes for detection of fragmented and un-fragmented bacterial DNA in dialytic fluid and demonstrated that Bellco Selecta resin is capable to retain these type of bacterial DNA. This article is protected by copyright. All rights reserved.
Journal of Nephrology, 2014
In myeloma cast nephropathy, fast reduction of serum free light chain (FLC) levels correlates wit... more In myeloma cast nephropathy, fast reduction of serum free light chain (FLC) levels correlates with renal recovery. Recently, extracorporeal treatments using filters with a high-molecular weight cut-off have been successfully used for FLC removal. However, using these new filters, high cost and elevated albumin leakage are common drawbacks. We studied a new and cheaper therapeutic approach with adsorbent resins to evaluate its efficacy. We treated four patients, affected by dialysis-dependent acute kidney injury (AKI) due to biopsy proven de novo FLC myeloma cast nephropathy. Each patient underwent bortezomib chemotherapy and extracorporeal treatment with the supra-hemodiafiltration with endogenous reinfusion (HFR) technique (Supra-HFR, Bellco Mirandola, Modena, Italy). Supra-HFR is a kind of hemodiafiltration that utilizes separated convection, diffusion and adsorption. The sorbent cartridge has a high affinity for FLC (both κ and λ) but is able to re-infuse albumin, avoiding the need for albumin perfusions. Supra HFR treatments (4 h each) were carried out for eight consecutive days and then every other day. All patients showed a significant reduction of serum FLC, whereas serum albumin concentration remained unchanged. Renal function recovered in three out of four patients. FLC removal with adsorbent resins represents an effective therapeutic strategy that does not require replacement with albumin .
Nephrology Dialysis Transplantation, 2014
Introduction and Aims: Online hemodiafiltration (HDF) with high-volume substitution fluid is an o... more Introduction and Aims: Online hemodiafiltration (HDF) with high-volume substitution fluid is an optimal way to remove uremic substances ranging widely in molecular size from small to low molecular weight (MW). Post-dilution HDF is the most efficient infusion mode to obtain maximum clearance.Mid-dilution infusion is an interesting alternative that represents simultaneous pre-and post-dilution infusion modes and could be a highly effective technique to remove uremic toxins, avoiding the disadvantages of pre-and post-dilutional modes.The aim of this multicentric study was to compare mid-dilution (MID) with post-dilution HDF (POST) by evaluating their efficiency in removing different middle MW (MMW) and protein bound uremic toxins. Methods: We performed a cross-over study including 158 Patients from 21 centres. All patients were randomized in two different groups for three months: group A (1 month MID-1 month POST-1 month MID) and group B (1 month POST-1 month MID-1 month POST). 64 patients were excluded from the final analysis because of incomplete data. The reduction rate (RR) of middle and protein bound molecules were centrally determined from serum samples as well as the second generation Daugirdas Kt/Vd. Albumin loss was carried out for both groups.Unpaired t student test was performed using GraphPad prism version 4.00 for Windows. SP411 Baseline patients characteristics and RRF survival by group Age years Weight kg baseline GFR mL/min baseline Kt/V per session deltaGFR ml/min/y mean RRF survival months 2-y cum surv % G1 N=48
Abstract. Making crystals by design is the paradigm of crystal engineering. The main goal is that... more Abstract. Making crystals by design is the paradigm of crystal engineering. The main goal is that of obtaining and controlling the collective properties of a crystalline material from the convolution of the physical and chemical properties of the individual building blocks (whether ...
Journal of Materials Science: Materials in Medicine, 2014
Your article is protected by copyright and all rights are held exclusively by Springer Science +B... more Your article is protected by copyright and all rights are held exclusively by Springer Science +Business Media New York. This e-offprint is for personal use only and shall not be selfarchived in electronic repositories. If you wish to self-archive your article, please use the accepted manuscript version for posting on your own website. You may further deposit the accepted manuscript version in any repository, provided it is only made publicly available 12 months after official publication or later and provided acknowledgement is given to the original source of publication and a link is inserted to the published article on Springer's website. The link must be accompanied by the following text: "The final publication is available at link.springer.com".
Annali di Chimica, 2005
The complex formation between the dioxouranium (VI) and the oxalate ions has been investigated by... more The complex formation between the dioxouranium (VI) and the oxalate ions has been investigated by measuring the potential of a glass electrode, at 25.00 degrees C, in 1 and 3 M NaClO4, at lower acidities than 10(-4.5) M, in order to favour the formation of (mixed) ternary species. The upper limits of concentration of all the analytical parameters have been imposed by the modest solubility of Na2C2O4 in the ionic media. The experimental measurements at different ionic strengths have been treated by means of the computerised least square programme LETAGROP - ETITR. Ternary complexes of general composition (p, q, r) are formed according to reaction (1), in addition to the already reported binary complexes. pUO2(2+) + qH2O + rC2O4(2-) &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;==&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt; (UO2)p(OH)q(C2O4)r(2p-q-2r)(+) + qH+ (1). The stoichiometric compositions of the ternary species are (1, 1, 1), (2, 4, 2), (2, 2, 4). Their formation constants, expressed in molality, obtained in the two ionic media, are listed below. [table: see text]. For reasons discussed in the present work in the last column only the value of the constant in 1 M ionic medium is reported for the species (2, 4, 2). Additional evidence on the stoichiometric composition of the species formed is afforded by the mass-spectrometric measurements, collected in solutions of known composition.
Nephrology Dialysis Transplantation, 2014
Introduction and Aims: A high level of fibroblast growth factor 23 (FGF23) is a risk factor for m... more Introduction and Aims: A high level of fibroblast growth factor 23 (FGF23) is a risk factor for mortality, and recent studies have linked FGF23 to parameters of volume homeostasis and an increased risk of heart failure. In hemodialysis (HD), a large ultrafiltration volume (UFV) is also associated with an increased mortality risk. We aimed to investigate whether circulating FGF23 levels and ultrafiltration volume are related in a cohort of stable HD patients. Methods: Post-hoc analysis on a prospective cohort study of 104 HD patients, median age 66 (interquartile range 51-75) years, dialysis vintage 25.0 (8.5-51.2) months, who underwent a standard four-hour HD session at the first session of the week. Blood samples were drawn at onset of HD. Plasma C-terminal FGF23 was determined by ELISA. Residual renal function (RRF) and Kt/V were extracted from patient records. We used uni-and multivariate linear regression to assess the association between UFV and FGF23. Natural log (Ln)-transformation was applied when appropriate. Results: At start of the HD session the median FGF23 level was 7535 [interquartile range 3276-13433] RU/mL. Mean UFV throughout the HD session was 2561 (standard deviation ±771) mL. In univariate analysis, natural log-transformed (Ln) FGF23 levels correlated with UFV (Figure 1), and also with serum phosphate (R=0.587, P<0.001), age (R=-0.384 P<0.001), and Ln Kt/V (R=-0.252 P=0.01), but not significantly with calcium (R=0.167 P=0.09). Multivariate linear regression analysis revealed a consistent strong association between Ln FGF23 AND UFV (St. β 0.385, P<0.001), in a model adjusted for serum phosphate (Standardized β 0.451, P<0.001) and serum calcium (St. β 0.222, P=0.002; model R2 52%). Age, gender, dialysis vintage, Kt/V, systolic and diastolic blood pressure did not contribute to this model. The association between FGF23 and UFV was independent of serum phosphate (Figures 1, 2). Patients with relevant residual renal function had a trend for lower FGF23 levels (Mann-Whitney P=0.04, Spearman's Rho-0.230 P=0.04), however RRF was no correlate of FGF23 in multivariate analysis (St. β-0.111, P=0.16).
Thermochimica Acta, 2002
The coordination chemistry of dioxouranium(VI) with organic ligands may represent an useful model... more The coordination chemistry of dioxouranium(VI) with organic ligands may represent an useful model to describe the migration of radionuclides in natural fluids. A new dioxouranium(VI) ternary complex of formula Na4(UO2)2(OH)4(C2O4)2 was precipitated at pH≅8. It was characterized by elemental analysis, ICP and IR spectroscopy, thermogravimetry (TG) and coupled TG–IR evolved gas analysis. Potentiometric measurements in the solution suggest that the