Glaucia Santelli - Academia.edu (original) (raw)

Papers by Glaucia Santelli

Brazilian Journal of Radiation Sciences, Jul 4, 2019

Food irradiation has been approved in more than 60 countries for many applications in a wide vari... more Food irradiation has been approved in more than 60 countries for many applications in a wide variety of foods. 2-Alkylcyclobutanones (2-ACBs) are the only known radiolytic products formed when foods containing fatty acids are irradiated. Despite the importance of food irradiation, the toxicological potential of 2-ACBs in irradiated food is still no fully understood. In this study we investigated the cytotoxic effects of irradiated palmitic and stearic fatty acids byproducts, 2-dDCB and 2-tDCB, in hepatic cell lines (HepG2, BRL3A and HTC). The cytotoxic effects of 2-dDCB and 2-tDCB were evaluated at 100, 300 and 500 μM for 24 and 48 hours and the cell viability was measured using the MTS assay. While no toxicity was observed for 2-tDCB in all cells for all tested conditions, 2-dDCB was found to be toxic to BRL3A cells (at 100 µM after 48 hours) and HTC cells (at 24 hours in all tested concentrations). HepG2 cells on the other hand, were found to be resistant to 2-dDCB-induced toxicity. Overall our data shows that the byproduct 2-tDCB is not toxic for hepatic cells while 2-dDCB can be used has a potential marker for food irradiation-induced toxicity.

Revista brasileira de genetica, 1987

... Fotocópia. Mori, Lyria. experimental, Documentos relacionados. Id: 40441. Autor: Oliveira, Al... more ... Fotocópia. Mori, Lyria. experimental, Documentos relacionados. Id: 40441. Autor: Oliveira, AliceReis de; Mori, Lyria; Machado Santelli, Gláucia M. Título: Diphenylhydantoin effects in Balb C mouse bone marrow cells: cytogenetic aspects. Fonte: Rev. bras. genét = Braz. ...

Comparative Immunology Microbiology and Infectious Diseases, 2011

The interaction between avian mycoplasmas and their hosts is far more complex than might be antic... more The interaction between avian mycoplasmas and their hosts is far more complex than might be anticipated from the apparent structural and functional simplicity of the wall-less prokaryotes involved. Mycoplasmas are usually found intimately adhered to epithelial host cells from which their nutritional requirements are readily obtained [1]. However, the internalisation of mycoplasmas of human origin by non-phagocytic cells is well known, and host cell invasion by several non-human Mollicutes, including the avian pathogen M. gallisepticum (Mg; Mycoplasmatales, Mollicutes) [2], has been reported.

Molecular Immunology, Oct 1, 2008

Research Square (Research Square), Apr 19, 2023

Several drug screening campaigns identi ed Calpeptin as a drug candidate against SARS-CoV-2. Init... more Several drug screening campaigns identi ed Calpeptin as a drug candidate against SARS-CoV-2. Initially reported to target the viral main protease (Mpro), its moderate activity in Mpro inhibition assays hints at a second target. Indeed, we show that Calpeptin is an extremely potent cysteine cathepsin inhibitor, a nding additionally supported by X-ray crystallography. Cell infection assays proved Calpeptin's e cacy against SARS-CoV-2. Treatment of SARS-CoV-2-infected Golden Syrian hamsters with sulfonated Calpeptin at a dose of 1 mg/kg body weight signi cantly reduces the viral load in the trachea. Our results show that the inhibition of cathepsins, a protein family of the host organism, is a promising approach for the treatment of SARS-CoV-2 and potentially other viral infections. An intrinsic advantage in targeting host proteins is their mutational stability in contrast to highly mutable viral targets. the collaborative network between the Universities São Paulo (USP) and Hamburg (UHH) via the UHH-USP-FAPESP Sprint Project 2019 (FAPESP 2019/00899-0). We also acknowledge the Butantan Institute (M.V.A fellowship) and the Brazilian Ministry of Education, CAPES (88887.508739/2020-00, 000).

Open Biology, 2021

Gap junctions mediate communication between adjacent cells and are fundamental to the development... more Gap junctions mediate communication between adjacent cells and are fundamental to the development and homeostasis in multicellular organisms. In invertebrates, gap junctions are formed by transmembrane proteins called innexins. Gap junctions allow the passage of small molecules through an intercellular channel, between a cell and another adjacent cell. The dipteran Rhynchosciara americana has contributed to studying the biology of invertebrates and the study of the interaction and regulation of genes during biological development. Therefore, this paper aimed to study the R. americana innexin-2 by molecular characterization, analysis of the expression profile and cellular localization. The molecular characterization results confirm that the message is from a gap junction protein and analysis of the expression and cellular localization profile shows that innexin-2 can participate in many physiological processes during the development of R. americana .

This work presents the first study showing how photobiomodulation (PBM) significantly increases c... more This work presents the first study showing how photobiomodulation (PBM) significantly increases cellular and tissue repair and elucidating the role of PBM with low-level laser as a possible new therapy in pathologies in COVID-19-associated cytokine storm syndrome from a zebrafish model. Our results demonstrate new strategies for treating SARS-COV-2 using PBM to modulate the expression of the genes and metabolites involved in inflammatory processes. These metabolic alterations show that the r-Spike led to disturbance in the energetic and inflammatory system, corroborating with the severe clinical conditions of human patients. Furthermore, PBM decreased the gene expression levels of pro-inflammatory cytokines such as il1b, il6, tnfa, and nfkbiab, and of factors involved in oxidative stress (romo1) and energy metabolism (slc2a1a, coa1), in various tissues, promoting an anti-inflammatory response. In summary, our study suggests that PBM may have a positive role in treating cytokine stor...

Brazilian Journal of Medical and Biological Research, 2012

Hepatocellular carcinoma (HCC) is the third highest cause of cancer death worldwide. In general, ... more Hepatocellular carcinoma (HCC) is the third highest cause of cancer death worldwide. In general, the disease is diagnosed at an advanced stage when potentially curative therapies are no longer feasible. For this reason, it is very important to develop new therapeutic approaches. Retinoic acid (RA) is a natural derivative of vitamin A that regulates important biological processes including cell proliferation and differentiation. In vitro studies have shown that RA is effective in inhibiting growth of HCC cells; however, responsiveness to treatment varies among different HCC cell lines. The objective of the present study was to determine if the combined use of RA (0.1 µM) and cAMP (1 mM), an important second messenger, improves the responsiveness of HCC cells to RA treatment. We evaluated the proliferative behavior of an HCC cell line (HTC) and the expression profile of genes related to cancer signaling pathway (ERK and GSK-3β) and liver differentiation [E-cadherin, connexin 26 (Cx26), and connexin 32 (Cx32)]. RA and cAMP were effective in inhibiting the proliferation of HTC cells independently of combined use. However, when a mixture of RA and cAMP was used, the signals concerning the degree of cell differentiation were increased. As demonstrated by Western blot, the treatment increased E-cadherin, Cx26, Cx32 and Ser9-GSK-3β (inactive form) expression while the expression of Cx43, Tyr216-GSK-3β (active form) and phosphorylated ERK decreased. Furthermore, telomerase activity was inhibited along treatment. Taken together, the results showed that the combined use of RA and cAMP is more effective in inducing differentiation of HTC cells.

BMC Cell Biology, 2002

BACKGROUND: Down syndrome is the most frequent genetic disorder in humans. Rare cases involving p... more BACKGROUND: Down syndrome is the most frequent genetic disorder in humans. Rare cases involving partial trisomy of chromosome 21 allowed a small chromosomal region common to all carriers, called Down Syndrome Critical Region (DSCR), to be determined. The DSCR1 gene was identified in this region and is expressed preferentially in the brain, heart and skeletal muscle. Recent studies have shown

Due to their general cytotoxic effects, anticancer drugs pose a great risk to aquatic species, ye... more Due to their general cytotoxic effects, anticancer drugs pose a great risk to aquatic species, yet they have been among the least studied groups of drugs regarding their environmental impact. The present study aimed to identify the effects of the antileukemia drug cytarabine, also known as cytosine arabinoside (ara-C), on the development and neurobehavior of zebrafish larvae. Zebrafish eggs were exposed to cytarabine to assess survival, embryonic malformations, locomotor activity, DNA damage, and biochemical alterations. No mortality, teratogenicity, or genotoxicity was detected in zebrafish larvae up to 100 mg L-1. However, cytarabine decreased the total distance traveled by zebrafish under dark conditions, suggesting depression-like behavior in treated larvae. Furthermore, it was demonstrated that cytarabine exposure increased lipid peroxidation (LPO) and acetylcholinesterase (AChE) activity, while it inhibited catalase (CAT) activity. Multivariate analysis suggested that both ACh...

Journal Biomedical and Biopharmaceutical Research, 2015

The aim of this study was the development, rheological behaviour determination, and sensory analy... more The aim of this study was the development, rheological behaviour determination, and sensory analysis of cosmetic formulations containing glucan biopolymer (Manihot esculenta), a tensor agent that was proposed to produce an immediate lifting and smoothing effect. For this purpose, formulations were developed and supplemented or not with 4 % of hydrolysed Manihot esculenta tuber extract and submitted to preliminary stability tests. These formulations were evaluated in terms of rheological behaviour over 90 days. Sensory analysis was carried out through a research with 20 cosmetic consumers who answered a questionnaire regarding their perception to the cosmetic qualities. The formulations presented pseudoplastic behavior and were considered stable in the physical stability studies, with the exception of the gel formulation based on Ammonium Acryloyldimethyltaurate/VP Copolymer. The formulations were well evaluated in the sensory parameters. The gel formulations based on Polyacrylamide, C13-14 Isoparaffin, and Laureth-7 were stable and presented the best sensory profile in some evaluated parameters, such as spreadability, smoothness and skin moisturizing, and can be considered an appropriate vehicle for formulations containing hydrolysed Manihot esculenta tuber extract.

Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 1996

In the present study we analyzed the induction of micronuclei (MN) by colchicine, at different tr... more In the present study we analyzed the induction of micronuclei (MN) by colchicine, at different treatment times, in four histogenetically different cell cultures: human skin fibroblasts (HSF), bovine skin fibroblasts (BSF), bovine bladder fibroblasts (BBF) and human skin epithelial cells (HK), developed and characterized in our laboratory. The frequencies of dead cells, nuclear budding and mitotic index were also evaluated. The HSF and BSF cell lines showed similar frequencies of micronucleated cells (4,7% and 4,9%, respectively) at 96 h of treatment time. The BBF cell line showed the lowest frequency of micronucleated cells (2,6%) and HK did not show any MN. The studied cell lines differed in their responses to colchicine. The data revealed the relevance of utilization of other end-points as growth curves, dead cells, mitotic index and other nuclear alterations for accurate analysis of the effect of agents that disturb cell cycle or are cytotoxic.