Golam Sarower - Academia.edu (original) (raw)

Papers by Golam Sarower

Research paper thumbnail of Growth and Survival of Goldfish (Carassius auratus) Juveniles Fed Tubifex, Custard Meal and Commercial Feeds

Basic and Applied Sciences - Scientific Journal of King Faisal University, 2022

An experiment was conducted to investigate suitable feed for Carassius auratus juveniles. 30-day-... more An experiment was conducted to investigate suitable feed for Carassius auratus juveniles. 30-day-old juveniles (3.31±0.14 cm and 0.84±0.3 g) were reared in glass aquaria (50×30×30 cm 3) at stocking density of 10 juveniles/aquaria in triplicates. The juveniles were fed four different feeds, Tubifex, egg custard, commercial feed, and aquarium feed, twice daily until satiation. After 28 days of trial, fish fed custard and Tubifex had significantly higher survival rates (91% and 88%) than those fed aquarium feed (81%, P < 0.05) and commercial fish feed (75%, P < 0.05). The final length, weight, absolute growth rate and metabolic growth rate were significantly varied among the treatments (P < 0.05). However, no significant differences were evident in weight gain and SGR (P > 0.05). The fish fed custard and Tubifex were comparable in terms of growth performance (P > 0.05) while a significantly lower growth rate was found in fish fed commercial feed (P < 0.05). Fish fed Tubifex and custard had significantly higher protein content (15.28% and 14.86%, respectively) than those fed commercial fish feed (13.41%) and aquarium feed (12.21%). The overall findings suggest that the formulated egg custard can promote growth and survival in goldfish.

Research paper thumbnail of Genetic population structure of mud crab (Scylla olivacea) in the Bay of Bengal inferred from mitochondrial DNA and microsatellite markers

The increasing exploitation of Scylla olivacea, one of the most commercially consumed mud crabs i... more The increasing exploitation of Scylla olivacea, one of the most commercially consumed mud crabs inhabiting mangroves and estuaries of the Bay of Bengal has been seriously experiencing a constant decline of its population. Information on its genetic population structure is crucial for the conservation of S. olivacea. Here, partial sequences of the mitochondrial cytochrome c oxidase subunit I (COI) gene and ten microsatellite loci were used to estimate population genetics from four localities in the Bay of Bangladesh. Genotypes of mitochondrial DNA (mtDNA) were identified with an 848 bp fragment sequence of COI gene, defining 32 different haplotypes from H1 to H32. H4 was the most frequently observed haplotype, existing in all four localities, while the majority of haplotypes were rare ones, existing in only one individual. Neutrality tests and mismatch analysis conferred a potential population expansion event for this crab species. Diploid genotypes were determined with microsatellit...

Research paper thumbnail of Author response for "Profiling of bioactive compounds and antioxidant activity of aquatic weed Ipomoea aquatica

Research paper thumbnail of 無脊椎動物における遊離D-アミノ酸の分布,代謝および生理機能(第56回大会シンポジウム(I))

Research paper thumbnail of Growth and Survival of Goldfish (Carassius auratus) Juveniles Fed Tubifex, Custard Meal and Commercial Feeds

Basic and Applied Sciences - Scientific Journal of King Faisal University, 2022

An experiment was conducted to investigate suitable feed for Carassius auratus juveniles. 30-day-... more An experiment was conducted to investigate suitable feed for Carassius auratus juveniles. 30-day-old juveniles (3.31±0.14 cm and 0.84±0.3 g) were reared in glass aquaria (50×30×30 cm3) at stocking density of 10 juveniles/aquaria in triplicates. The juveniles were fed four different feeds, Tubifex, egg custard, commercial feed, and aquarium feed, twice daily until satiation. After 28 days of trial, fish fed custard and Tubifex had significantly higher survival rates (91% and 88%) than those fed aquarium feed (81%, P < 0.05) and commercial fish feed (75%, P < 0.05). The final length, weight, absolute growth rate and metabolic growth rate were significantly varied among the treatments (P < 0.05). However, no significant differences were evident in weight gain and SGR (P > 0.05). The fish fed custard and Tubifex were comparable in terms of growth performance (P > 0.05) while a significantly lower growth rate was found in fish fed commercial feed (P < 0.05). Fish fed Tu...

Research paper thumbnail of 無脊椎動物における遊離D-アミノ酸の分布,代謝および生理機能(第56回大会シンポジウム(I))

Research paper thumbnail of Effect of Environmental Stresses on Lipid Production by Tetraselmis Sp. And Modified Extraction Methods for Lipid Recovery in Aspect of Prospective Biofuel Production

Microalgae are known to be the most potential biomass for production of biofuel. Different enviro... more Microalgae are known to be the most potential biomass for production of biofuel. Different environmental parameters like salinity, nitrogen deprivation and phosphorus deprivation were imposed on Tetraselmis sp. for examining their effects on algal growth and lipid accumulation. Additionally, various pretreatments namely thermal, ultrasound and ultraviolet were checked to enhance the lipid recovery from algal cells. Highest biomass was achieved from 35‰ salinity condition. Highest lipid content was found in thermal pretreated nitrogen deprived algal stock yielding 69.73% of dry weight. Lipid accumulation was highest in nitrogen deprivation yielding 42.83% of dry weight. Highest amount of lipid was recovered from thermal pretreatment yielding45.67%of dry weight. Thus this work has aided an optimized way to enhance biofuel production in terms of optimum biomass, lipid induction as well as lipid recovery from microalgal cell.

Research paper thumbnail of Monitoring of genetic variation in mono-sex tilapia stocks using randomly amplified polymorphic DNA markers in some private hatcheries of Bangladesh

Egyptian journal of aquatic biology and fisheries, 2021

Bangladesh is sel-sufficient in fish production since 2018 with a total production of 4.27 millio... more Bangladesh is sel-sufficient in fish production since 2018 with a total production of 4.27 million metric ton (DoF, 2018). Of the total fish, tilapia (Oreochromis niloticus) production of more than 3.8 lakh metric ton has brought the country as the 4 th largest tilapia producer in the world (DoF, 2018). In fact, rapid growth rate, stress tolerance ARTICLE INFO

Research paper thumbnail of Polymerase Chain Reaction Technique for Rapid Check of Virulency of Escherichia Coli from Shrimp Farms

Polymerase Chain Reaction (PCR) technique allows the detection of the most enterovirulent Escheri... more Polymerase Chain Reaction (PCR) technique allows the detection of the most enterovirulent Escherichia coli strains: enterotoxigenic (ETEC), enteroinvasive (EIEC), enterohaemorrhagic (EHEC) and enteropathogenic (EPEC). However, PCR efficiency depends on the primer design, target samples and environmental factors. The aim of the present study was therefore to detect enterovirulent strains of E. coli in the four different shrimp farms of Dumuria, Khulna, Bangladesh. Water, sediment and shrimp were examined from each farm. Six pairs of oligonucleotide specific primers were used for the amplification and thereby detection of target genes viz. the alkaline phophatase (phoA), the heat-labile toxin1 (hlt1), heat-labile toxin2 (hlt2), heat-stable toxin1(hst1), verotoxin (vt) and attachment and effacement (eae) virulence genes. Finally, PCR products were analyzed and detection of different strains was confirmed by agarose gel electrophoresis using DNA markers. The results revealed the presenc...

Research paper thumbnail of Study on the Diversity and Seasonal Variation of Zooplankton in a Brood Pond, Bangladesh

Roy, U., Shaha,B.K., Mazhabuddin,Kh., Haque, M.F., Sarower, M.G. 2010. Study on the diversity and... more Roy, U., Shaha,B.K., Mazhabuddin,Kh., Haque, M.F., Sarower, M.G. 2010. Study on the diversity and seasonal variation of zooplankton in a brood pond, Bangladesh. marine.res.aqua. 1(1):30-37. The present study was designed to estimate zooplankton abundance qualitatively and quantitatively in carp brood fish culture systems. Plankton samples were collected from Gallamari fisheries extension & training centre farm, Batiaghata, Khulna, one time in a month. A total 6 groups of zooplankton were found from the study ponds. The identified zooplanktons were under six orders namely copepoda, rotifera, cladocera, ostracoda, crustacean larvae and bryozoa. Numbers of zooplankton groups were recorded to be the highest in December. In the brood pond copepod was the dominant order. Among various orders of zooplankton, the percentages of copepoda, rotifera, cladocera, ostracoda and crustacean larvae brysozoa in brood fish culture system were 55%, 8%, 25%, 9%, 2% and 1% respectively. Copepoda was domi...

Research paper thumbnail of The Effects of Water Quality Additives on Bacterial Load and Production in Thai Climbing Perch (Anabas testudineus Bloch, 1792) Culture Pond

International Journal of Fisheries and Aquatic Studies, 2015

This research was carried out to investigate the effects of commercial water quality additives na... more This research was carried out to investigate the effects of commercial water quality additives named aqua cleaner and deotop on bacterial load in Thai climbing perch (Anabas testudineus) culture pond as well as to observe the effects of the additives on the production of climbing perch. The study was conducted under four treatments that were designated as T1(5ml/decimal aqua cleaner and 61g/decimal deotop,T2(10ml/decimal aqua cleaner and 122g/decimal deotop),T3(20ml/decimal aqua cleaner and 244gm/decimal deotop),T4(control without any additives) and each of them with three replicates.T4([(4.99±0.03)×10 4 cfu/ml water], [(5.49±0.09)×10 6 cfu/g soil]) and T3([(3.80±0.58) ×104cfu/ml water], [(4.99±0.23) ×106cfu/g soil]) revealed the highest and lowest average bacterial load in both water and soil of the culture pond respectively whereas the highest production of perch was found in T2(10.23 kg/decimal) followed by T1(8.44 kg/decimal), T3(8.39 kg/decimal), and T4(7.71 kg/decimal).The res...

Research paper thumbnail of Sexual variation in fillet yield and phenotypic relationship between some economic traits of bata (Labeo bata, Hamilton, 1822)

Relationships between different traits of Bata (Labeo bata) were estimated from a sample of 300 i... more Relationships between different traits of Bata (Labeo bata) were estimated from a sample of 300 individuals (164 male and 136 female). The mean standard length, body width and body depth of the samples were: for male 17.69±3.88 cm, 4.27±1.09 cm and 2.57±0.75 cm and for female 16.78±4.06 cm, 4.09±1.06cm and 2.48±0.81cm respectively. The average total weight for male was 120.75±69.52g and for female was102.21±69.70. Data were analyzed for total weight (TW), trunk weight (TrW) standard length (L), body depth (BD), dress-out weight (DW) and fillet weight (FW).The study was conducted from April to October in 2011. The average fillet % and dress-out % for male were 59.49±7.03 and 79.86±3.72 and for female were 58.89±8.10 and 76.41±5.20 respectively. All the fishes had normal body shape having mean condition factor as 0.99±0.067 for male (range 0.84-1.17) and 0.98±0.08 for female (range 0.78-1.26).There was no significant difference (p>0.05) in fillet %, dress-out % and condition factor between male and female. All the true traits were strongly and positively correlated (r>0.8) with one another. The result show that the relation between length and weight with condition factor is insignificant, (p= 0.61). Multivariate

Research paper thumbnail of Level of Shrimp Farming Knowledge of Farmers in Bagerhat

Ali, M.A.,Islam, M.R., Barman, S.K., Rahman, S.M.B and Sarower, M.G. 2009. Level of shrimp farmin... more Ali, M.A.,Islam, M.R., Barman, S.K., Rahman, S.M.B and Sarower, M.G. 2009. Level of shrimp farming knowledge of farmers in Bagerhat. j. innov.dev.strategy 3(3):35-42. Asurvey was conducted at six Upazila under Bagerhat district to determine ,the level of shrimp ,farming knowledge from July to September 2008. For which 120 farmers from the selected area were selected randomly. The knowledge status

Research paper thumbnail of Physiological Function and Metabolism of Free D-Alanine in Aquatic Animals

Biological and Pharmaceutical Bulletin, 2005

Aquatic crustaceans and some bivalve mollusks contain a large amount of free D-alanine (up to 100... more Aquatic crustaceans and some bivalve mollusks contain a large amount of free D-alanine (up to 100 m mmol/g wet wt.) in their tissues. Under high salinity stress, crustaceans and bivalve mollusks largely accumulate D-and Lalanine irrespective of species examined, together with L-glutamine, L-proline, and glycine of which increases are species dependent. These data indicate that D-alanine is one of the major compatible osmolytes responsible for the intracellular isosmotic regulation in the tissues of crustaceans and bivalves. Alanine racemase has been proven to catalyze the interconversion of D-and L-alanine in these invertebrates. The enzyme has been isolated to homogeneity from the muscle of black tiger prawn Penaeus monodon and its cDNA has been cloned from the muscle and hepatopancreas of kuruma prawn Penaeus japonicus for the first time in eukaryotes other than yeast. Several fish species fed on crustaceans and mollusks contain D-amino acid and D-aspartate oxidases that catalyze the decomposition of D-amino acids. A cDNA of D-amino acid oxidase has been cloned from the hepatopancreas of omnivorous common carp Cyprinus carpio. During oral administration of free D-alanine to carp, the activity and mRNA of D-amino acid oxidase increased rapidly in hepatopancreas and the increases were highest in intestine followed by hepatopancreas and kidney. These data suggest that D-amino acid oxidase is inducible in carp and an important enzyme responsible for the efficient utilization of carbon skeleton of D-alanine in their feeds.

Research paper thumbnail of Molecular characterization of d-amino acid oxidase from common carp Cyprinus carpio and its induction with exogenous free d-alanine

Archives of Biochemistry and Biophysics, 2003

A full-length cDNA encoding D D-amino acid oxidase (DAO, EC 1.4.3.3) was cloned and sequenced fro... more A full-length cDNA encoding D D-amino acid oxidase (DAO, EC 1.4.3.3) was cloned and sequenced from the hepatopancreas of carp fed a diet supplemented with D D-alanine. This clone contained an open reading frame encoding 347 amino acid residues. The deduced amino acid sequence exhibited about 60 and 19-29% identity to mammalian and microbial DAOs, respectively. The expression of full-length carp DAO cDNA in Escherichia coli resulted in a significant level of protein with DAO activity. In carp fed the diet with D D-alanine for 14 days, DAO mRNA was strongly expressed in intestine followed by hepatopancreas and kidney, but not in muscle. During D D-alanine administration, DAO gene was expressed quickly in hepatopancreas with the increase of DAO activity. The inducible nature of carp DAO indicates that it plays an important physiological role in metabolizing exogenous D D-alanine that is abundant in their prey invertebrates, crustaceans, and mollusks.

Research paper thumbnail of Evaluation of the Efficacies of Selected Medicinal Plants on Pathogenic Escherichia Coli Strains

International Journal of Research Studies in Biosciences, 2016

The medicinal plants are used in traditional treatments to cure variety of diseases for thousands... more The medicinal plants are used in traditional treatments to cure variety of diseases for thousands of year. The aim of this study was to identify such plants with antimicrobial efficacy for controlling some pathogenic Escherichia coli strains. The locally available plants viz., garlic (Allium sativum), turmeric (Curcuma longa), crown flower (Calotropis gigentica), and Indian lilac (Azadirachta indica) were selected for the study. Ethanol extracts of these plants at two different doses (500-and 1000µg/disc) were examined against pathogenic E. coli strains using disc diffusion method as compared with standard kanamycin (30µg/disc.) Antibacterial activity was determined by measuring growth inhibition zones of E. coli strains. E. coli 119-EAE was more sensitive to all experimented extracts of medicinal plants except crown flower at the rate of 1000 µg/disc than other three strains. At the both concentration, the ethanol mixed extracts of crown flower and Indian lilac was most effective towards all E. coli strains. These extracts demonstrated the highest antibacterial activity against E. coli 119-EAE inhibiting its growth (21mm zone of diameter) at the dose of 1000µg/disc compared to standard antibiotic, kanamycin followed by Indian lilac and turmeric. The extracts of garlic exhibited the lowest inhibitory responses to all test organisms apart from E. coli 119-EAE strains. Furthermore, for in vivo challenge test of shrimps (Penaeus monodon), by using ethanolic mixed extracts of crown flower and Indian lilac the lowest average bacterial load of E. coli was observed to be 2.11×10 4 CFU/g at the concentration of 1000µg/µl in muscle of shrimp reducing from 3.09×10 4 CFU/g found without applying extracts as control. These results suggested that there was strong antagonistic activity of ethanolic mixed extract of crown flower and Indian lilac against the strain of E. coli and it can be used in favor of proper fish health management controlling the risk of contamination from pathogenic E. coli in shrimp as important source of drugs.

Research paper thumbnail of Isolation and Identification of Slime Forming Staphylococcus aureus from Raw Cow Milk and their Antibiotic Susceptibility Pattern

South Asian Journal of Agriculture

Staphylococcus aureus is an emerging pathogen from dairy animals that can form biofilms in the ud... more Staphylococcus aureus is an emerging pathogen from dairy animals that can form biofilms in the udder of dairy cows affected by mastitis. The use of antibiotics in dairy farms leads to a decrease in bacteria, finally making healthy food. The aim of this study was to determine the occurrence, antibiotic susceptibility, and biofilm forming ability of S. aureus isolated from cow milk. A total of fifteen raw milk samples were collected from Mohammadpur, Narayangonj, Savar, Bosila, Siddheshwari and Norshingdi dairy farms for isolation of S. aureus. All the samples were highly loaded with Staphylococcus and the total staphylococcal count ranged from 105 to 106 cfu ml-1. Biochemical tests (TSI, catalase, oxidase, motility, MR/VP etc.) were performed for the confirmation of the Staphylococcal isolates. The 100% resistance was observed against oxacillin, erythromycin, cotrimoxazole, amoxicillin, cefixime and vancomycin. In contrast to these results, the isolates showed 100% sensitivity to gen...

Research paper thumbnail of Antioxidant Potential and Nutrient Content of Selected Small Indigenous Species of Fish

The antioxidant level and nutrients in sixteen small indigenous fish species (SIS) of Bangladesh;... more The antioxidant level and nutrients in sixteen small indigenous fish species (SIS) of Bangladesh; eight marine and eight freshwater were measured by Thin Layer Chromatography (TLC) and 2, 2-Diphenyl 1-picrylhydrazil, 95% (DPPH) free radical scavenging method. The IC 50 determined by DPPH varied from 327.04±0.06 µg/ml to 1888.21±0.10 µg/ml of wet weight. The highest antioxidant activity was observed in Heteropneustes fossilis followed by Mystus gulio, Hemirhamphus gaimardi, Mystus vittatus, Megalaspis cordyla, Silonia silondia, Colisa fasciatus, Amblypharyngodon mola, Oxygaster phulo, Gobioides anguillaris, Chela laubuca, Plotosus canius, Channa orientalis, Mugil cephalus, Coilia dussumieri and Tetraodon cutcutia. The protein and lipid contents of the selected SIS ranged between 21.43 to 8.59%, and 7.22 to 1.75%, respectively. The study suggests the presence of potent antioxidant and appreciable amount of nutrients in selected fish samples. The top four potential SIS species consist ...

Research paper thumbnail of Distribution and characteristics of D-amino acid and D-aspartate oxidases in fish tissues

Journal of experimental zoology. Part A, Comparative experimental biology, 2003

The distributions of D-amino acid oxidase (D-AAO, EC 1.4.3.3) and D-aspartate oxidase (D-AspO, EC... more The distributions of D-amino acid oxidase (D-AAO, EC 1.4.3.3) and D-aspartate oxidase (D-AspO, EC 1.4.3.1) activities were examined on several tissues of various fish species. Both enzyme activities were commonly high in kidney and liver and low in intestine with some exceptions. After oral administration of D-alanine at 5 micromol /g body weight(-1)day(-1) to carp for 30 days, D-AAO activity increased by about 8-, 3-, and 1.5-fold in intestine, hepatopancreas, and kidney, respectively, whereas no increase was found in brain. In contrast, oral administration of D-glutamate or D-aspartate did not show any increase of D-AspO activity in any tissues. D-AAO and D-AspO of common carp kidney and hepatopancreas were subcellularly localized in peroxisomes, as clarified in mammals. D-proline was the best substrate for D-AAO in rainbow trout kidney, common carp kidney, and hepatopancreas, followed by D-alanine and D-phenylalanine. N-methyl-D-aspartate was the best substrate for D-AspO in rain...

Research paper thumbnail of Antioxidant Activity of Ethanol and Lipophilic Extracts of Common Fruity Vegetables in Bangladesh

International Journal of Food Properties, 2014

The amount of phenolics, flavonoids, and anthocyanins in ethanol extracts and antioxidant activit... more The amount of phenolics, flavonoids, and anthocyanins in ethanol extracts and antioxidant activity of both ethanol and lipophilic extracts of common fruity vegetables in Bangladesh were studied. Among the ethanol extracts of 15 fruity vegetables, M. oleifera had the highest total polyphenol content (85.05 mg gallic acid equivalent/g extract) followed by L. acutangula (61.74 mg gallic acid equivalent/g extract) and A. esculentus (48.92 mg gallic acid equivalent/g extract). L. acutangula had high a content of flavonoids (14.46 mg (+)catechin equivalent/g extract), which was almost similar to L. siceraria (13.67 mg catechin equivalent/g extract) followed by A. esculentus (11.95 mg catechin equivalent/g extract) and S. melongena (11.42 mg catechin equivalent/g extract). Highest anthocyanins content was in F. hispida (2.22 µmol/g extract) followed by S. melongena (1.04 µmol/g extract). Ethanol extracts of A. esculentus, F. hispida, L. acutangula, L. siceraria, and S. melongena exhibited high DPPH free radical scavenging activity with IC 50 of 70.4, 64.9, 70.4, 64.9, and 94.3 µg/mL respectively, whereas for the same lipophilic extracts of F. hispida and S. melongena showed lowest (37 µg/mL) IC 50 followed by M. oleifera (47.6 µg/mL), L. siceraria (57.5 µg/mL), and A. esculentus (63.3 µg/mL). These vegetables also showed high reducing powers, NO scavenging and total antioxidant capacity. Therefore, the top five potential fruity vegetables consist of both hydrophilic and lipophilic antioxidant(s), the order being F. hispida > M. oleifera > A. esculentus, L. acutangula > L. siceraria > and S. melongena.

Research paper thumbnail of Growth and Survival of Goldfish (Carassius auratus) Juveniles Fed Tubifex, Custard Meal and Commercial Feeds

Basic and Applied Sciences - Scientific Journal of King Faisal University, 2022

An experiment was conducted to investigate suitable feed for Carassius auratus juveniles. 30-day-... more An experiment was conducted to investigate suitable feed for Carassius auratus juveniles. 30-day-old juveniles (3.31±0.14 cm and 0.84±0.3 g) were reared in glass aquaria (50×30×30 cm 3) at stocking density of 10 juveniles/aquaria in triplicates. The juveniles were fed four different feeds, Tubifex, egg custard, commercial feed, and aquarium feed, twice daily until satiation. After 28 days of trial, fish fed custard and Tubifex had significantly higher survival rates (91% and 88%) than those fed aquarium feed (81%, P < 0.05) and commercial fish feed (75%, P < 0.05). The final length, weight, absolute growth rate and metabolic growth rate were significantly varied among the treatments (P < 0.05). However, no significant differences were evident in weight gain and SGR (P > 0.05). The fish fed custard and Tubifex were comparable in terms of growth performance (P > 0.05) while a significantly lower growth rate was found in fish fed commercial feed (P < 0.05). Fish fed Tubifex and custard had significantly higher protein content (15.28% and 14.86%, respectively) than those fed commercial fish feed (13.41%) and aquarium feed (12.21%). The overall findings suggest that the formulated egg custard can promote growth and survival in goldfish.

Research paper thumbnail of Genetic population structure of mud crab (Scylla olivacea) in the Bay of Bengal inferred from mitochondrial DNA and microsatellite markers

The increasing exploitation of Scylla olivacea, one of the most commercially consumed mud crabs i... more The increasing exploitation of Scylla olivacea, one of the most commercially consumed mud crabs inhabiting mangroves and estuaries of the Bay of Bengal has been seriously experiencing a constant decline of its population. Information on its genetic population structure is crucial for the conservation of S. olivacea. Here, partial sequences of the mitochondrial cytochrome c oxidase subunit I (COI) gene and ten microsatellite loci were used to estimate population genetics from four localities in the Bay of Bangladesh. Genotypes of mitochondrial DNA (mtDNA) were identified with an 848 bp fragment sequence of COI gene, defining 32 different haplotypes from H1 to H32. H4 was the most frequently observed haplotype, existing in all four localities, while the majority of haplotypes were rare ones, existing in only one individual. Neutrality tests and mismatch analysis conferred a potential population expansion event for this crab species. Diploid genotypes were determined with microsatellit...

Research paper thumbnail of Author response for "Profiling of bioactive compounds and antioxidant activity of aquatic weed Ipomoea aquatica

Research paper thumbnail of 無脊椎動物における遊離D-アミノ酸の分布,代謝および生理機能(第56回大会シンポジウム(I))

Research paper thumbnail of Growth and Survival of Goldfish (Carassius auratus) Juveniles Fed Tubifex, Custard Meal and Commercial Feeds

Basic and Applied Sciences - Scientific Journal of King Faisal University, 2022

An experiment was conducted to investigate suitable feed for Carassius auratus juveniles. 30-day-... more An experiment was conducted to investigate suitable feed for Carassius auratus juveniles. 30-day-old juveniles (3.31±0.14 cm and 0.84±0.3 g) were reared in glass aquaria (50×30×30 cm3) at stocking density of 10 juveniles/aquaria in triplicates. The juveniles were fed four different feeds, Tubifex, egg custard, commercial feed, and aquarium feed, twice daily until satiation. After 28 days of trial, fish fed custard and Tubifex had significantly higher survival rates (91% and 88%) than those fed aquarium feed (81%, P < 0.05) and commercial fish feed (75%, P < 0.05). The final length, weight, absolute growth rate and metabolic growth rate were significantly varied among the treatments (P < 0.05). However, no significant differences were evident in weight gain and SGR (P > 0.05). The fish fed custard and Tubifex were comparable in terms of growth performance (P > 0.05) while a significantly lower growth rate was found in fish fed commercial feed (P < 0.05). Fish fed Tu...

Research paper thumbnail of 無脊椎動物における遊離D-アミノ酸の分布,代謝および生理機能(第56回大会シンポジウム(I))

Research paper thumbnail of Effect of Environmental Stresses on Lipid Production by Tetraselmis Sp. And Modified Extraction Methods for Lipid Recovery in Aspect of Prospective Biofuel Production

Microalgae are known to be the most potential biomass for production of biofuel. Different enviro... more Microalgae are known to be the most potential biomass for production of biofuel. Different environmental parameters like salinity, nitrogen deprivation and phosphorus deprivation were imposed on Tetraselmis sp. for examining their effects on algal growth and lipid accumulation. Additionally, various pretreatments namely thermal, ultrasound and ultraviolet were checked to enhance the lipid recovery from algal cells. Highest biomass was achieved from 35‰ salinity condition. Highest lipid content was found in thermal pretreated nitrogen deprived algal stock yielding 69.73% of dry weight. Lipid accumulation was highest in nitrogen deprivation yielding 42.83% of dry weight. Highest amount of lipid was recovered from thermal pretreatment yielding45.67%of dry weight. Thus this work has aided an optimized way to enhance biofuel production in terms of optimum biomass, lipid induction as well as lipid recovery from microalgal cell.

Research paper thumbnail of Monitoring of genetic variation in mono-sex tilapia stocks using randomly amplified polymorphic DNA markers in some private hatcheries of Bangladesh

Egyptian journal of aquatic biology and fisheries, 2021

Bangladesh is sel-sufficient in fish production since 2018 with a total production of 4.27 millio... more Bangladesh is sel-sufficient in fish production since 2018 with a total production of 4.27 million metric ton (DoF, 2018). Of the total fish, tilapia (Oreochromis niloticus) production of more than 3.8 lakh metric ton has brought the country as the 4 th largest tilapia producer in the world (DoF, 2018). In fact, rapid growth rate, stress tolerance ARTICLE INFO

Research paper thumbnail of Polymerase Chain Reaction Technique for Rapid Check of Virulency of Escherichia Coli from Shrimp Farms

Polymerase Chain Reaction (PCR) technique allows the detection of the most enterovirulent Escheri... more Polymerase Chain Reaction (PCR) technique allows the detection of the most enterovirulent Escherichia coli strains: enterotoxigenic (ETEC), enteroinvasive (EIEC), enterohaemorrhagic (EHEC) and enteropathogenic (EPEC). However, PCR efficiency depends on the primer design, target samples and environmental factors. The aim of the present study was therefore to detect enterovirulent strains of E. coli in the four different shrimp farms of Dumuria, Khulna, Bangladesh. Water, sediment and shrimp were examined from each farm. Six pairs of oligonucleotide specific primers were used for the amplification and thereby detection of target genes viz. the alkaline phophatase (phoA), the heat-labile toxin1 (hlt1), heat-labile toxin2 (hlt2), heat-stable toxin1(hst1), verotoxin (vt) and attachment and effacement (eae) virulence genes. Finally, PCR products were analyzed and detection of different strains was confirmed by agarose gel electrophoresis using DNA markers. The results revealed the presenc...

Research paper thumbnail of Study on the Diversity and Seasonal Variation of Zooplankton in a Brood Pond, Bangladesh

Roy, U., Shaha,B.K., Mazhabuddin,Kh., Haque, M.F., Sarower, M.G. 2010. Study on the diversity and... more Roy, U., Shaha,B.K., Mazhabuddin,Kh., Haque, M.F., Sarower, M.G. 2010. Study on the diversity and seasonal variation of zooplankton in a brood pond, Bangladesh. marine.res.aqua. 1(1):30-37. The present study was designed to estimate zooplankton abundance qualitatively and quantitatively in carp brood fish culture systems. Plankton samples were collected from Gallamari fisheries extension & training centre farm, Batiaghata, Khulna, one time in a month. A total 6 groups of zooplankton were found from the study ponds. The identified zooplanktons were under six orders namely copepoda, rotifera, cladocera, ostracoda, crustacean larvae and bryozoa. Numbers of zooplankton groups were recorded to be the highest in December. In the brood pond copepod was the dominant order. Among various orders of zooplankton, the percentages of copepoda, rotifera, cladocera, ostracoda and crustacean larvae brysozoa in brood fish culture system were 55%, 8%, 25%, 9%, 2% and 1% respectively. Copepoda was domi...

Research paper thumbnail of The Effects of Water Quality Additives on Bacterial Load and Production in Thai Climbing Perch (Anabas testudineus Bloch, 1792) Culture Pond

International Journal of Fisheries and Aquatic Studies, 2015

This research was carried out to investigate the effects of commercial water quality additives na... more This research was carried out to investigate the effects of commercial water quality additives named aqua cleaner and deotop on bacterial load in Thai climbing perch (Anabas testudineus) culture pond as well as to observe the effects of the additives on the production of climbing perch. The study was conducted under four treatments that were designated as T1(5ml/decimal aqua cleaner and 61g/decimal deotop,T2(10ml/decimal aqua cleaner and 122g/decimal deotop),T3(20ml/decimal aqua cleaner and 244gm/decimal deotop),T4(control without any additives) and each of them with three replicates.T4([(4.99±0.03)×10 4 cfu/ml water], [(5.49±0.09)×10 6 cfu/g soil]) and T3([(3.80±0.58) ×104cfu/ml water], [(4.99±0.23) ×106cfu/g soil]) revealed the highest and lowest average bacterial load in both water and soil of the culture pond respectively whereas the highest production of perch was found in T2(10.23 kg/decimal) followed by T1(8.44 kg/decimal), T3(8.39 kg/decimal), and T4(7.71 kg/decimal).The res...

Research paper thumbnail of Sexual variation in fillet yield and phenotypic relationship between some economic traits of bata (Labeo bata, Hamilton, 1822)

Relationships between different traits of Bata (Labeo bata) were estimated from a sample of 300 i... more Relationships between different traits of Bata (Labeo bata) were estimated from a sample of 300 individuals (164 male and 136 female). The mean standard length, body width and body depth of the samples were: for male 17.69±3.88 cm, 4.27±1.09 cm and 2.57±0.75 cm and for female 16.78±4.06 cm, 4.09±1.06cm and 2.48±0.81cm respectively. The average total weight for male was 120.75±69.52g and for female was102.21±69.70. Data were analyzed for total weight (TW), trunk weight (TrW) standard length (L), body depth (BD), dress-out weight (DW) and fillet weight (FW).The study was conducted from April to October in 2011. The average fillet % and dress-out % for male were 59.49±7.03 and 79.86±3.72 and for female were 58.89±8.10 and 76.41±5.20 respectively. All the fishes had normal body shape having mean condition factor as 0.99±0.067 for male (range 0.84-1.17) and 0.98±0.08 for female (range 0.78-1.26).There was no significant difference (p>0.05) in fillet %, dress-out % and condition factor between male and female. All the true traits were strongly and positively correlated (r>0.8) with one another. The result show that the relation between length and weight with condition factor is insignificant, (p= 0.61). Multivariate

Research paper thumbnail of Level of Shrimp Farming Knowledge of Farmers in Bagerhat

Ali, M.A.,Islam, M.R., Barman, S.K., Rahman, S.M.B and Sarower, M.G. 2009. Level of shrimp farmin... more Ali, M.A.,Islam, M.R., Barman, S.K., Rahman, S.M.B and Sarower, M.G. 2009. Level of shrimp farming knowledge of farmers in Bagerhat. j. innov.dev.strategy 3(3):35-42. Asurvey was conducted at six Upazila under Bagerhat district to determine ,the level of shrimp ,farming knowledge from July to September 2008. For which 120 farmers from the selected area were selected randomly. The knowledge status

Research paper thumbnail of Physiological Function and Metabolism of Free D-Alanine in Aquatic Animals

Biological and Pharmaceutical Bulletin, 2005

Aquatic crustaceans and some bivalve mollusks contain a large amount of free D-alanine (up to 100... more Aquatic crustaceans and some bivalve mollusks contain a large amount of free D-alanine (up to 100 m mmol/g wet wt.) in their tissues. Under high salinity stress, crustaceans and bivalve mollusks largely accumulate D-and Lalanine irrespective of species examined, together with L-glutamine, L-proline, and glycine of which increases are species dependent. These data indicate that D-alanine is one of the major compatible osmolytes responsible for the intracellular isosmotic regulation in the tissues of crustaceans and bivalves. Alanine racemase has been proven to catalyze the interconversion of D-and L-alanine in these invertebrates. The enzyme has been isolated to homogeneity from the muscle of black tiger prawn Penaeus monodon and its cDNA has been cloned from the muscle and hepatopancreas of kuruma prawn Penaeus japonicus for the first time in eukaryotes other than yeast. Several fish species fed on crustaceans and mollusks contain D-amino acid and D-aspartate oxidases that catalyze the decomposition of D-amino acids. A cDNA of D-amino acid oxidase has been cloned from the hepatopancreas of omnivorous common carp Cyprinus carpio. During oral administration of free D-alanine to carp, the activity and mRNA of D-amino acid oxidase increased rapidly in hepatopancreas and the increases were highest in intestine followed by hepatopancreas and kidney. These data suggest that D-amino acid oxidase is inducible in carp and an important enzyme responsible for the efficient utilization of carbon skeleton of D-alanine in their feeds.

Research paper thumbnail of Molecular characterization of d-amino acid oxidase from common carp Cyprinus carpio and its induction with exogenous free d-alanine

Archives of Biochemistry and Biophysics, 2003

A full-length cDNA encoding D D-amino acid oxidase (DAO, EC 1.4.3.3) was cloned and sequenced fro... more A full-length cDNA encoding D D-amino acid oxidase (DAO, EC 1.4.3.3) was cloned and sequenced from the hepatopancreas of carp fed a diet supplemented with D D-alanine. This clone contained an open reading frame encoding 347 amino acid residues. The deduced amino acid sequence exhibited about 60 and 19-29% identity to mammalian and microbial DAOs, respectively. The expression of full-length carp DAO cDNA in Escherichia coli resulted in a significant level of protein with DAO activity. In carp fed the diet with D D-alanine for 14 days, DAO mRNA was strongly expressed in intestine followed by hepatopancreas and kidney, but not in muscle. During D D-alanine administration, DAO gene was expressed quickly in hepatopancreas with the increase of DAO activity. The inducible nature of carp DAO indicates that it plays an important physiological role in metabolizing exogenous D D-alanine that is abundant in their prey invertebrates, crustaceans, and mollusks.

Research paper thumbnail of Evaluation of the Efficacies of Selected Medicinal Plants on Pathogenic Escherichia Coli Strains

International Journal of Research Studies in Biosciences, 2016

The medicinal plants are used in traditional treatments to cure variety of diseases for thousands... more The medicinal plants are used in traditional treatments to cure variety of diseases for thousands of year. The aim of this study was to identify such plants with antimicrobial efficacy for controlling some pathogenic Escherichia coli strains. The locally available plants viz., garlic (Allium sativum), turmeric (Curcuma longa), crown flower (Calotropis gigentica), and Indian lilac (Azadirachta indica) were selected for the study. Ethanol extracts of these plants at two different doses (500-and 1000µg/disc) were examined against pathogenic E. coli strains using disc diffusion method as compared with standard kanamycin (30µg/disc.) Antibacterial activity was determined by measuring growth inhibition zones of E. coli strains. E. coli 119-EAE was more sensitive to all experimented extracts of medicinal plants except crown flower at the rate of 1000 µg/disc than other three strains. At the both concentration, the ethanol mixed extracts of crown flower and Indian lilac was most effective towards all E. coli strains. These extracts demonstrated the highest antibacterial activity against E. coli 119-EAE inhibiting its growth (21mm zone of diameter) at the dose of 1000µg/disc compared to standard antibiotic, kanamycin followed by Indian lilac and turmeric. The extracts of garlic exhibited the lowest inhibitory responses to all test organisms apart from E. coli 119-EAE strains. Furthermore, for in vivo challenge test of shrimps (Penaeus monodon), by using ethanolic mixed extracts of crown flower and Indian lilac the lowest average bacterial load of E. coli was observed to be 2.11×10 4 CFU/g at the concentration of 1000µg/µl in muscle of shrimp reducing from 3.09×10 4 CFU/g found without applying extracts as control. These results suggested that there was strong antagonistic activity of ethanolic mixed extract of crown flower and Indian lilac against the strain of E. coli and it can be used in favor of proper fish health management controlling the risk of contamination from pathogenic E. coli in shrimp as important source of drugs.

Research paper thumbnail of Isolation and Identification of Slime Forming Staphylococcus aureus from Raw Cow Milk and their Antibiotic Susceptibility Pattern

South Asian Journal of Agriculture

Staphylococcus aureus is an emerging pathogen from dairy animals that can form biofilms in the ud... more Staphylococcus aureus is an emerging pathogen from dairy animals that can form biofilms in the udder of dairy cows affected by mastitis. The use of antibiotics in dairy farms leads to a decrease in bacteria, finally making healthy food. The aim of this study was to determine the occurrence, antibiotic susceptibility, and biofilm forming ability of S. aureus isolated from cow milk. A total of fifteen raw milk samples were collected from Mohammadpur, Narayangonj, Savar, Bosila, Siddheshwari and Norshingdi dairy farms for isolation of S. aureus. All the samples were highly loaded with Staphylococcus and the total staphylococcal count ranged from 105 to 106 cfu ml-1. Biochemical tests (TSI, catalase, oxidase, motility, MR/VP etc.) were performed for the confirmation of the Staphylococcal isolates. The 100% resistance was observed against oxacillin, erythromycin, cotrimoxazole, amoxicillin, cefixime and vancomycin. In contrast to these results, the isolates showed 100% sensitivity to gen...

Research paper thumbnail of Antioxidant Potential and Nutrient Content of Selected Small Indigenous Species of Fish

The antioxidant level and nutrients in sixteen small indigenous fish species (SIS) of Bangladesh;... more The antioxidant level and nutrients in sixteen small indigenous fish species (SIS) of Bangladesh; eight marine and eight freshwater were measured by Thin Layer Chromatography (TLC) and 2, 2-Diphenyl 1-picrylhydrazil, 95% (DPPH) free radical scavenging method. The IC 50 determined by DPPH varied from 327.04±0.06 µg/ml to 1888.21±0.10 µg/ml of wet weight. The highest antioxidant activity was observed in Heteropneustes fossilis followed by Mystus gulio, Hemirhamphus gaimardi, Mystus vittatus, Megalaspis cordyla, Silonia silondia, Colisa fasciatus, Amblypharyngodon mola, Oxygaster phulo, Gobioides anguillaris, Chela laubuca, Plotosus canius, Channa orientalis, Mugil cephalus, Coilia dussumieri and Tetraodon cutcutia. The protein and lipid contents of the selected SIS ranged between 21.43 to 8.59%, and 7.22 to 1.75%, respectively. The study suggests the presence of potent antioxidant and appreciable amount of nutrients in selected fish samples. The top four potential SIS species consist ...

Research paper thumbnail of Distribution and characteristics of D-amino acid and D-aspartate oxidases in fish tissues

Journal of experimental zoology. Part A, Comparative experimental biology, 2003

The distributions of D-amino acid oxidase (D-AAO, EC 1.4.3.3) and D-aspartate oxidase (D-AspO, EC... more The distributions of D-amino acid oxidase (D-AAO, EC 1.4.3.3) and D-aspartate oxidase (D-AspO, EC 1.4.3.1) activities were examined on several tissues of various fish species. Both enzyme activities were commonly high in kidney and liver and low in intestine with some exceptions. After oral administration of D-alanine at 5 micromol /g body weight(-1)day(-1) to carp for 30 days, D-AAO activity increased by about 8-, 3-, and 1.5-fold in intestine, hepatopancreas, and kidney, respectively, whereas no increase was found in brain. In contrast, oral administration of D-glutamate or D-aspartate did not show any increase of D-AspO activity in any tissues. D-AAO and D-AspO of common carp kidney and hepatopancreas were subcellularly localized in peroxisomes, as clarified in mammals. D-proline was the best substrate for D-AAO in rainbow trout kidney, common carp kidney, and hepatopancreas, followed by D-alanine and D-phenylalanine. N-methyl-D-aspartate was the best substrate for D-AspO in rain...

Research paper thumbnail of Antioxidant Activity of Ethanol and Lipophilic Extracts of Common Fruity Vegetables in Bangladesh

International Journal of Food Properties, 2014

The amount of phenolics, flavonoids, and anthocyanins in ethanol extracts and antioxidant activit... more The amount of phenolics, flavonoids, and anthocyanins in ethanol extracts and antioxidant activity of both ethanol and lipophilic extracts of common fruity vegetables in Bangladesh were studied. Among the ethanol extracts of 15 fruity vegetables, M. oleifera had the highest total polyphenol content (85.05 mg gallic acid equivalent/g extract) followed by L. acutangula (61.74 mg gallic acid equivalent/g extract) and A. esculentus (48.92 mg gallic acid equivalent/g extract). L. acutangula had high a content of flavonoids (14.46 mg (+)catechin equivalent/g extract), which was almost similar to L. siceraria (13.67 mg catechin equivalent/g extract) followed by A. esculentus (11.95 mg catechin equivalent/g extract) and S. melongena (11.42 mg catechin equivalent/g extract). Highest anthocyanins content was in F. hispida (2.22 µmol/g extract) followed by S. melongena (1.04 µmol/g extract). Ethanol extracts of A. esculentus, F. hispida, L. acutangula, L. siceraria, and S. melongena exhibited high DPPH free radical scavenging activity with IC 50 of 70.4, 64.9, 70.4, 64.9, and 94.3 µg/mL respectively, whereas for the same lipophilic extracts of F. hispida and S. melongena showed lowest (37 µg/mL) IC 50 followed by M. oleifera (47.6 µg/mL), L. siceraria (57.5 µg/mL), and A. esculentus (63.3 µg/mL). These vegetables also showed high reducing powers, NO scavenging and total antioxidant capacity. Therefore, the top five potential fruity vegetables consist of both hydrophilic and lipophilic antioxidant(s), the order being F. hispida > M. oleifera > A. esculentus, L. acutangula > L. siceraria > and S. melongena.