Eleni Gomes - Academia.edu (original) (raw)

Papers by Eleni Gomes

Research paper thumbnail of Chlamydia trachomatis in gynaecological infections in Luanda, Angola

Sexually Transmitted Infections, 1995

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Research paper thumbnail of 15 Environmental data requirements of maritime operations in the Benguela coastal ocean

Large Marine Ecosystems, 2006

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Research paper thumbnail of D-glyceraldehyde-3-phosphate dehydrogenase from HeLa cells--1. Purification and properties of the enzyme

Comparative biochemistry and physiology. B, Comparative biochemistry, 1992

1. D-GPDH from HeLa cells was isolated and purified. 2. Some basic kinetic constants are reported... more 1. D-GPDH from HeLa cells was isolated and purified. 2. Some basic kinetic constants are reported. 3. Sodium dodecyl polyacrylamide gel electrophoresis gave a single band with a molecular weight of approximately 36 K. 4. ATP and NADH inhibit competitively enzyme activity. 5. Comparative catalytic properties of GPDH from normal and tumor cells were effectuated.

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Research paper thumbnail of Evaluation of Solid and Submerged Fermentations for the Production of Cyclodextrin Glycosyltransferase by Paenibacillus campinasensis H69-3 and Characterization of Crude Enzyme

Twenty-Seventh Symposium on Biotechnology for Fuels and Chemicals, 2006

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Research paper thumbnail of Optimization of cyclodextrin glucanotransferase production from Bacillus clausii E16 in submerged fermentation using response surface methodology

Cyclodextrin glucanotransferase production from Bacillus clausii E16, a new bacteria isolated fro... more Cyclodextrin glucanotransferase production from Bacillus clausii E16, a new bacteria isolated from Brazilian soil samples was optimized in shake-flask cultures. A 2(4) full-factorial central composite design was performed to optimize the culture conditions, using a response surface methodology. The combined effect among the soluble starch concentration, the peptone concentration, the yeast extract concentration, and the initial pH value of the culture medium was investigated. The optimum concentrations of the components, determined by a 2(4) full-factorial central composite design, were 13.4 g/L soluble starch, 4.9 g/L peptone, 5.9 g/L yeast extract, and initial pH 10.1. Under these optimized conditions, the maximum cyclodextrin glucanotransferase activity was 5.9 U/mL after a 48-h fermentation. This yield was 68% higher than that obtained when the microorganism was cultivated in basal culture medium.

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Research paper thumbnail of Purification and characterization of an exo-polygalacturonase produced by Penicillium viridicatum RFC3 in solid-state fermentation

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Research paper thumbnail of Identification of genetic alterations in the glucoamylase Aspergillus awamori gene

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Research paper thumbnail of PRODUÇÃO DE GELÉIA DE JAMBOLÃO (Syzygium cumini Lamarck): PROCESSAMENTO, PARÂMETROS FÍSICO - QUÍMICOS E AVALIAÇÃO SENSORIAL1

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Research paper thumbnail of Purification and Characterization of Two Xylanases From Alkalophilic and Thermophilic Bacillus licheniformis 77-2

The alkalophilic bacteria Bacillus licheniformis 77-2 produces significant quantities of thermost... more The alkalophilic bacteria Bacillus licheniformis 77-2 produces significant quantities of thermostable cellulase-free xylanases. The crude xylanase was purified to apparent homogeneity by gel filtration (G-75) and ionic exchange chromatography (carboxymethyl sephadex, Q sepharose, and Mono Q), resulting in the isolation of two xylanases. The molecular masses of the enzymes were estimated to be 17 kDa (X-I) and 40 kDa (X-II),

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Research paper thumbnail of Utilização do resíduo líquido de indústria de processamento de suco de laranja como meio de cultura de Penicillium citrinum: depuração biológica do resíduo e produção de enzima

Quimica Nova, 1998

UTILIZATION OF RESIDUAL LIQUID ORANGE FROM JUICE PROCESSING AS CULTIVA-TION MEDIUM OF PENICILLIUM... more UTILIZATION OF RESIDUAL LIQUID ORANGE FROM JUICE PROCESSING AS CULTIVA-TION MEDIUM OF PENICILLIUM CITRINUM: BIOLOGICAL DEPURATION OF THE RESI-DUE AND ENZYME PRODUCTION. Penicillium citrinum grown in orange juice processing wastes ...

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Research paper thumbnail of Production and partial characterization of polygalacturonases produced by thermophilic Monascus sp N8 and by thermotolerant Aspergillus sp N12 on solid-state fermentation

Brazilian Journal of Microbiology, 2006

Polygalacturonases production by newly isolated Monascus sp N8 and Aspergillus sp N12 strains was... more Polygalacturonases production by newly isolated Monascus sp N8 and Aspergillus sp N12 strains was carried out in solid-state fermentation using mixtures of wheat bran, sugar cane bagasse and orange bagasse as carbon sources. The maximal activity values of exo-polygalacturonases (exo-Pg) from Monascus sp and Aspergillus sp were obtained using wheat bran/sugar cane bagasse/orange bagasse mixture (6.6 U/mL) and wheat bran/orange

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Research paper thumbnail of Enzymatic production by thermophilic fungi using agricultural wastes and ruminant diet as substrates

Journal of Biotechnology, 2007

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Research paper thumbnail of Production and evaluation of exogenous fibrolytic enzymes in presence of the rumen fluid and in vitro digestibility of tropical forage using enzymatic complex

Journal of Biotechnology, 2007

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Research paper thumbnail of Ligninolytic activity from newly isolated basidiomycete strains and effect of these enzymes on the azo dye orange II decolourisation

Annals of Microbiology, 2008

Wood-rotting fungi have the ability to degrade lignin by secreting ligninases, a promising enzyme... more Wood-rotting fungi have the ability to degrade lignin by secreting ligninases, a promising enzyme for degradation of environmental pollutants. Nine basidiomycete strains collected just outside the city of São José do Rio Preto, upstate São Paulo, Brazil, were evaluated for their manganese peroxidase (MnP), lignin peroxidase (LiP) and laccase production by solid-state fermentation on wheat bran.Datronia caperata SP381992,Polyporus tenuiculus SP381977

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Research paper thumbnail of Pectinolytic enzyme production by Bacillus species and their potential application on juice extraction

World Journal of Microbiology & Biotechnology, 2001

Five Bacillus strains isolated from decaying vegetable material were cultivated on wheat bran and... more Five Bacillus strains isolated from decaying vegetable material were cultivated on wheat bran and endo-polygalacturonases, exo-polygalacturonase and pectin lyase activities in the crude enzymatic solution obtained were determined. Highest activity was observed for all enzymes when fermentation was carried out at 28 °C, the highest activity values were obtained after 120 h of cultivation for exo-PG and after 48 h

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Research paper thumbnail of APLICAÇÃO DE XILANASE TERMOESTÁVEL DE Bacillus sp 1 O BRANQUEAMENTO DA POLPA KRAFT DE EUCALIPTUS APPLICATION OF THERMOSTABLE XYLANASE FROM Bacillus sp1 TO THE BLEACHING OF EUCALIPTUS KRAFT PULP

Xylanases are enzymes of great industrial interest, mainly that it concerns to pulp and paper ind... more Xylanases are enzymes of great industrial interest, mainly that it concerns to pulp and paper industries, where they can be used as aid in bleaching process causing the reducing of added chemical. Xylanases degrade xylan and improve the access of chemicals to the lignin. In this work the xylanase produced by one newly selected bacterium, Bacillus sp called strain N.

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Research paper thumbnail of Sugarcane bagasse saccharification by Myceliophthora sp F2.1.4 and Aspergillus fumigatus M7.3 enzymatic extracts

Journal of Biotechnology, 2010

ABSTRACT [P-B.108] Sugarcane bagasse saccharification by Myceliophthora sp F2.1.4and Aspergillus ... more ABSTRACT [P-B.108] Sugarcane bagasse saccharification by Myceliophthora sp F2.1.4and Aspergillus fumigatus M7.3 enzymatic extracts D.A. Bocchini-Martins∗, M.M.S. Moretti, M. Boscolo, R. Da Silva, E. Gomes IBILCE/UNESP, Brazil Keywords: Sugarcane bagasse; Saccharification; Myceliophthora; Aspergillus; Cellulases; Xylanases Cellulose and hemicellulose from sugarcane bagasse are a source of fermentable sugars. However, the complexity of lignocellulose represents an obstacle to sugarcane enzymatic degradation, so that pretreatments should be used to facilitate the enzymes access, improving hydrolysis. In this work, sugarcane bagasse saccharification by enzymatic extracts of two thermophilic fungi was evaluated. The enzymatic extracts from Myceliophthora sp F2.1.4 and Aspergillus fumigatus M7.3, obtained by solid state fermentation on lignocellulosic residues, were used and saccharification was performed at 50 ◦C, under 150 rpm, up to 72 h. Regarding “in natura” sugarcane bagasse, using the enzymatic extract from Myceliophthora sp F 2.1.4 (580 CMCase U/g of dry bagasse) or from A. fumigatus M7.3 (119,4 U/g of dry bagasse), 45,51 and 6,77mg of reducing sugar/g of bagasse were obtained after 48 h of hydrolysis, respectively. However, when bagasse pretreated with steam explosion was used, 80,78 and 31,8mg of reducing sugar/g of bagasse were obtained, under the same conditions, representing an increasing of 77,5 and 370% on sugar releasing, respectively. The increasing on hydrolysis time and on enzyme amount did not significantly improve saccharification. When CMCase and time of hydrolysis were fixed in 250 U/g of dry bagasse and 24 h, 57,5 and 22,8mg of reducing sugar/g of bagasse were obtained using A. fumigatus M7.3 or Myceliophthora sp F2.1.4 enzymatic extracts, respectively. Fromthese datawecan infer that the steam explosion pretreatment facilitated the enzymatic hydrolysis and that the enzymatic extract of A. fumigatus seems to be better than that from Myceliophthora on sugarcane bagasse hydrolysis. doi:10.1016/j.jbiotec.2010.08.461

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Research paper thumbnail of Purification and Characterization of a Cyclomaltodextrin Glucanotransferase From Paenibacillus campinasensis Strain H69-3

Applied Biochemistry and Biotecnology, 2007

A cyclomaltodextrin glucanotransferase (E.C. 2.4.1.19) from a newly isolated alkalophilic and mod... more A cyclomaltodextrin glucanotransferase (E.C. 2.4.1.19) from a newly isolated alkalophilic and moderately thermophilic Paenibacillus campinasensis strain H69-3 was purified as a homogeneous protein from culture supernatant. Cyclomaltodextrin glucanotransferase was produced during submerged fermentation at 45 degrees C and purified by gel filtration on Sephadex G50 ion exchange using a Q-Sepharose column and ion exchange using a Mono-Q column. The molecular weight of the purified enzyme was 70 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and the pI was 5.3. The optimum pH for enzyme activity was 6.5, and it was stable in the pH range 6.0-11.5. The optimum temperature was 65 degrees C at pH 6.5, and it was thermally stable up to 60 degrees C without substrate during 1 h in the presence of 10 mM CaCl(2). The enzyme activity increased in the presence of Co(2+), Ba(2+), and Mn(2+). Using maltodextrin as substrate, the K(m) and K(cat) were 1.65 mg/mL and 347.9 micromol/mg x min, respectively.

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Research paper thumbnail of Studies on the application of Myceliophthora thermophila JCP1-4 cellulases cocktail on sugarcane bagasse pretreated by different methods

Journal of Biotechnology, 2014

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Research paper thumbnail of Optimization of xylanase production by Bacillus circulans D1 in submerged fermentation using response surface methodology

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Research paper thumbnail of Chlamydia trachomatis in gynaecological infections in Luanda, Angola

Sexually Transmitted Infections, 1995

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Research paper thumbnail of 15 Environmental data requirements of maritime operations in the Benguela coastal ocean

Large Marine Ecosystems, 2006

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Research paper thumbnail of D-glyceraldehyde-3-phosphate dehydrogenase from HeLa cells--1. Purification and properties of the enzyme

Comparative biochemistry and physiology. B, Comparative biochemistry, 1992

1. D-GPDH from HeLa cells was isolated and purified. 2. Some basic kinetic constants are reported... more 1. D-GPDH from HeLa cells was isolated and purified. 2. Some basic kinetic constants are reported. 3. Sodium dodecyl polyacrylamide gel electrophoresis gave a single band with a molecular weight of approximately 36 K. 4. ATP and NADH inhibit competitively enzyme activity. 5. Comparative catalytic properties of GPDH from normal and tumor cells were effectuated.

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Research paper thumbnail of Evaluation of Solid and Submerged Fermentations for the Production of Cyclodextrin Glycosyltransferase by Paenibacillus campinasensis H69-3 and Characterization of Crude Enzyme

Twenty-Seventh Symposium on Biotechnology for Fuels and Chemicals, 2006

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Research paper thumbnail of Optimization of cyclodextrin glucanotransferase production from Bacillus clausii E16 in submerged fermentation using response surface methodology

Cyclodextrin glucanotransferase production from Bacillus clausii E16, a new bacteria isolated fro... more Cyclodextrin glucanotransferase production from Bacillus clausii E16, a new bacteria isolated from Brazilian soil samples was optimized in shake-flask cultures. A 2(4) full-factorial central composite design was performed to optimize the culture conditions, using a response surface methodology. The combined effect among the soluble starch concentration, the peptone concentration, the yeast extract concentration, and the initial pH value of the culture medium was investigated. The optimum concentrations of the components, determined by a 2(4) full-factorial central composite design, were 13.4 g/L soluble starch, 4.9 g/L peptone, 5.9 g/L yeast extract, and initial pH 10.1. Under these optimized conditions, the maximum cyclodextrin glucanotransferase activity was 5.9 U/mL after a 48-h fermentation. This yield was 68% higher than that obtained when the microorganism was cultivated in basal culture medium.

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Research paper thumbnail of Purification and characterization of an exo-polygalacturonase produced by Penicillium viridicatum RFC3 in solid-state fermentation

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Research paper thumbnail of Identification of genetic alterations in the glucoamylase Aspergillus awamori gene

Bookmarks Related papers MentionsView impact

Research paper thumbnail of PRODUÇÃO DE GELÉIA DE JAMBOLÃO (Syzygium cumini Lamarck): PROCESSAMENTO, PARÂMETROS FÍSICO - QUÍMICOS E AVALIAÇÃO SENSORIAL1

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Research paper thumbnail of Purification and Characterization of Two Xylanases From Alkalophilic and Thermophilic Bacillus licheniformis 77-2

The alkalophilic bacteria Bacillus licheniformis 77-2 produces significant quantities of thermost... more The alkalophilic bacteria Bacillus licheniformis 77-2 produces significant quantities of thermostable cellulase-free xylanases. The crude xylanase was purified to apparent homogeneity by gel filtration (G-75) and ionic exchange chromatography (carboxymethyl sephadex, Q sepharose, and Mono Q), resulting in the isolation of two xylanases. The molecular masses of the enzymes were estimated to be 17 kDa (X-I) and 40 kDa (X-II),

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Research paper thumbnail of Utilização do resíduo líquido de indústria de processamento de suco de laranja como meio de cultura de Penicillium citrinum: depuração biológica do resíduo e produção de enzima

Quimica Nova, 1998

UTILIZATION OF RESIDUAL LIQUID ORANGE FROM JUICE PROCESSING AS CULTIVA-TION MEDIUM OF PENICILLIUM... more UTILIZATION OF RESIDUAL LIQUID ORANGE FROM JUICE PROCESSING AS CULTIVA-TION MEDIUM OF PENICILLIUM CITRINUM: BIOLOGICAL DEPURATION OF THE RESI-DUE AND ENZYME PRODUCTION. Penicillium citrinum grown in orange juice processing wastes ...

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Research paper thumbnail of Production and partial characterization of polygalacturonases produced by thermophilic Monascus sp N8 and by thermotolerant Aspergillus sp N12 on solid-state fermentation

Brazilian Journal of Microbiology, 2006

Polygalacturonases production by newly isolated Monascus sp N8 and Aspergillus sp N12 strains was... more Polygalacturonases production by newly isolated Monascus sp N8 and Aspergillus sp N12 strains was carried out in solid-state fermentation using mixtures of wheat bran, sugar cane bagasse and orange bagasse as carbon sources. The maximal activity values of exo-polygalacturonases (exo-Pg) from Monascus sp and Aspergillus sp were obtained using wheat bran/sugar cane bagasse/orange bagasse mixture (6.6 U/mL) and wheat bran/orange

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Research paper thumbnail of Enzymatic production by thermophilic fungi using agricultural wastes and ruminant diet as substrates

Journal of Biotechnology, 2007

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Research paper thumbnail of Production and evaluation of exogenous fibrolytic enzymes in presence of the rumen fluid and in vitro digestibility of tropical forage using enzymatic complex

Journal of Biotechnology, 2007

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Research paper thumbnail of Ligninolytic activity from newly isolated basidiomycete strains and effect of these enzymes on the azo dye orange II decolourisation

Annals of Microbiology, 2008

Wood-rotting fungi have the ability to degrade lignin by secreting ligninases, a promising enzyme... more Wood-rotting fungi have the ability to degrade lignin by secreting ligninases, a promising enzyme for degradation of environmental pollutants. Nine basidiomycete strains collected just outside the city of São José do Rio Preto, upstate São Paulo, Brazil, were evaluated for their manganese peroxidase (MnP), lignin peroxidase (LiP) and laccase production by solid-state fermentation on wheat bran.Datronia caperata SP381992,Polyporus tenuiculus SP381977

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Research paper thumbnail of Pectinolytic enzyme production by Bacillus species and their potential application on juice extraction

World Journal of Microbiology & Biotechnology, 2001

Five Bacillus strains isolated from decaying vegetable material were cultivated on wheat bran and... more Five Bacillus strains isolated from decaying vegetable material were cultivated on wheat bran and endo-polygalacturonases, exo-polygalacturonase and pectin lyase activities in the crude enzymatic solution obtained were determined. Highest activity was observed for all enzymes when fermentation was carried out at 28 °C, the highest activity values were obtained after 120 h of cultivation for exo-PG and after 48 h

Bookmarks Related papers MentionsView impact

Research paper thumbnail of APLICAÇÃO DE XILANASE TERMOESTÁVEL DE Bacillus sp 1 O BRANQUEAMENTO DA POLPA KRAFT DE EUCALIPTUS APPLICATION OF THERMOSTABLE XYLANASE FROM Bacillus sp1 TO THE BLEACHING OF EUCALIPTUS KRAFT PULP

Xylanases are enzymes of great industrial interest, mainly that it concerns to pulp and paper ind... more Xylanases are enzymes of great industrial interest, mainly that it concerns to pulp and paper industries, where they can be used as aid in bleaching process causing the reducing of added chemical. Xylanases degrade xylan and improve the access of chemicals to the lignin. In this work the xylanase produced by one newly selected bacterium, Bacillus sp called strain N.

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Research paper thumbnail of Sugarcane bagasse saccharification by Myceliophthora sp F2.1.4 and Aspergillus fumigatus M7.3 enzymatic extracts

Journal of Biotechnology, 2010

ABSTRACT [P-B.108] Sugarcane bagasse saccharification by Myceliophthora sp F2.1.4and Aspergillus ... more ABSTRACT [P-B.108] Sugarcane bagasse saccharification by Myceliophthora sp F2.1.4and Aspergillus fumigatus M7.3 enzymatic extracts D.A. Bocchini-Martins∗, M.M.S. Moretti, M. Boscolo, R. Da Silva, E. Gomes IBILCE/UNESP, Brazil Keywords: Sugarcane bagasse; Saccharification; Myceliophthora; Aspergillus; Cellulases; Xylanases Cellulose and hemicellulose from sugarcane bagasse are a source of fermentable sugars. However, the complexity of lignocellulose represents an obstacle to sugarcane enzymatic degradation, so that pretreatments should be used to facilitate the enzymes access, improving hydrolysis. In this work, sugarcane bagasse saccharification by enzymatic extracts of two thermophilic fungi was evaluated. The enzymatic extracts from Myceliophthora sp F2.1.4 and Aspergillus fumigatus M7.3, obtained by solid state fermentation on lignocellulosic residues, were used and saccharification was performed at 50 ◦C, under 150 rpm, up to 72 h. Regarding “in natura” sugarcane bagasse, using the enzymatic extract from Myceliophthora sp F 2.1.4 (580 CMCase U/g of dry bagasse) or from A. fumigatus M7.3 (119,4 U/g of dry bagasse), 45,51 and 6,77mg of reducing sugar/g of bagasse were obtained after 48 h of hydrolysis, respectively. However, when bagasse pretreated with steam explosion was used, 80,78 and 31,8mg of reducing sugar/g of bagasse were obtained, under the same conditions, representing an increasing of 77,5 and 370% on sugar releasing, respectively. The increasing on hydrolysis time and on enzyme amount did not significantly improve saccharification. When CMCase and time of hydrolysis were fixed in 250 U/g of dry bagasse and 24 h, 57,5 and 22,8mg of reducing sugar/g of bagasse were obtained using A. fumigatus M7.3 or Myceliophthora sp F2.1.4 enzymatic extracts, respectively. Fromthese datawecan infer that the steam explosion pretreatment facilitated the enzymatic hydrolysis and that the enzymatic extract of A. fumigatus seems to be better than that from Myceliophthora on sugarcane bagasse hydrolysis. doi:10.1016/j.jbiotec.2010.08.461

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Research paper thumbnail of Purification and Characterization of a Cyclomaltodextrin Glucanotransferase From Paenibacillus campinasensis Strain H69-3

Applied Biochemistry and Biotecnology, 2007

A cyclomaltodextrin glucanotransferase (E.C. 2.4.1.19) from a newly isolated alkalophilic and mod... more A cyclomaltodextrin glucanotransferase (E.C. 2.4.1.19) from a newly isolated alkalophilic and moderately thermophilic Paenibacillus campinasensis strain H69-3 was purified as a homogeneous protein from culture supernatant. Cyclomaltodextrin glucanotransferase was produced during submerged fermentation at 45 degrees C and purified by gel filtration on Sephadex G50 ion exchange using a Q-Sepharose column and ion exchange using a Mono-Q column. The molecular weight of the purified enzyme was 70 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and the pI was 5.3. The optimum pH for enzyme activity was 6.5, and it was stable in the pH range 6.0-11.5. The optimum temperature was 65 degrees C at pH 6.5, and it was thermally stable up to 60 degrees C without substrate during 1 h in the presence of 10 mM CaCl(2). The enzyme activity increased in the presence of Co(2+), Ba(2+), and Mn(2+). Using maltodextrin as substrate, the K(m) and K(cat) were 1.65 mg/mL and 347.9 micromol/mg x min, respectively.

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Research paper thumbnail of Studies on the application of Myceliophthora thermophila JCP1-4 cellulases cocktail on sugarcane bagasse pretreated by different methods

Journal of Biotechnology, 2014

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Research paper thumbnail of Optimization of xylanase production by Bacillus circulans D1 in submerged fermentation using response surface methodology

Bookmarks Related papers MentionsView impact