Nikolai Gor - Academia.edu (original) (raw)

Papers by Nikolai Gor

†Both authors contributed equally to the presented work. ††The opinions or assertions contained h... more †Both authors contributed equally to the presented work. ††The opinions or assertions contained herein are the authors’ private views and are not to be construed as official or reflecting the views of the Uniformed Services University of the Health Sciences or the US Department of defence. No conflicts of interest were declared.

Current Topics on Military Medicine, 2021

American Journal of Physiology-Lung Cellular and Molecular Physiology, 1997

Blast overpressure (BOP) is a phenomenon that describes the instantaneous rise in atmospheric pre... more Blast overpressure (BOP) is a phenomenon that describes the instantaneous rise in atmospheric pressure above ambient, resulting from the firing of large caliber weapons or from military or civilian explosions. Exposure to BOP results in injury to the gas-filled organs, such as the lungs, which exhibit a contusion-type injury. We examined the effects of BOP in rats at 5 and 60 min after exposure to a low-level BOP (62 +/- 3 kPa). The exposure was found to cause oxidative stress in the lung that was characterized by 1) a 3.5-fold decrease in total antioxidant reserves, 2) a depletion of the major water-soluble antioxidants ascorbate and glutathione (GSH) by 50 and 75%, respectively, 3) a depletion of lipid-soluble antioxidant vitamin E by 30%, 4) a 2.5-fold increase of fluorescent end products of lipid peroxidation, and 5) an increased methemoglobin (metHb) content at 60 min after exposure. To elucidate the role of released hemoglobin (Hb) in blast-induced oxidative stress, we studied...

International Journal of Molecular Sciences, 2017

Mediators of Inflammation, 2017

Ionizing radiation combined with trauma tissue injury (combined injury, CI) results in greater mo... more Ionizing radiation combined with trauma tissue injury (combined injury, CI) results in greater mortality and H-ARS than radiation alone (radiation injury, RI), which includes thrombocytopenia. The aim of this study was to determine whether increases in numbers of thrombocytes would improve survival and mitigate H-ARS after CI. We observed in mice that WBC and platelets remained very low in surviving RI animals that were given 9.5 Gy60Co-γ-photon radiation, whereas only lymphocytes and basophils remained low in surviving CI mice that were irradiated and then given skin wounds. Numbers of RBC and platelets, hemoglobin concentrations, and hematocrit values remained low in surviving RI and CI mice. CI induced 30-day mortality higher than RI. Radiation delayed wound healing by approximately 14 days. Treatment with a thrombopoietin receptor agonist, Alxn4100TPO, after CI improved survival, mitigated body-weight loss, and reduced water consumption. Though this therapy delayed wound-healing...

International Journal of Immunopathology and Pharmacology, 2010

Ionizing irradiation causes damage and functional failure of irradiation-sensitive systems and ti... more Ionizing irradiation causes damage and functional failure of irradiation-sensitive systems and tissues such as small intestine. The molecular mechanisms underlying inflammatory and adaptive responses to acute irradiation damage are poorly understood. Using a mouse model of total-body γ-irradiation, we assessed the irradiation response of crypt host-defense Paneth cells by measuring α-defensin 4 (AD4) expression and correlated the gathered data with activation of the caspase-1/IL-1β inflammatory signaling cascade. The irradiation injury was produced in CD2F1 mice exposed to 9.25 Gy γ-radiation. This dose resulted in 85–100% mortality at the 15th day post-irradiation. Small intestine tissue samples were collected at the 7th day post-irradiation. Assessment of irradiation-associated pro-inflammatory alterations in small intestine tissue and expression of AD4 in Paneth cells was conducted using confocal immunofluorescence imaging, transmission electron microscopy (TEM), light microscopy...

Autophagy in Current Trends in Cellular Physiology and Pathology, 2016

Circulation, Oct 31, 2007

Journal of Chromatography B: Biomedical Sciences and Applications, 1993

A rapid and simple technique using reversed-phase high-performance liquid chromatography with ele... more A rapid and simple technique using reversed-phase high-performance liquid chromatography with electrochemical detection (HPLC-ED) was developed for the determination of nitrite. Standard solutions of sodium nitrite produced a linear current response over the concentration range 1.5-30 microM. The chromatographic peaks of sodium nitrite were superimposable with peaks obtained after injection into the chromatograph of supernatants from rat cerebellar synaptosomes. The concentrations of nitrite in synaptosomes measured with HPLC-ED were similar to those found with a widely used colorimetric procedure. Therefore, this new method may represent a useful means of measuring nitrite in biological samples.

Free Radical and Antioxidant Protocols

ABSTRACT

Comparative Biochemistry and Physiology Part C: Comparative Pharmacology, 1986

Macroautophagy (mATG) is a major lysosomal mechanism for the bulk degradation of cytoplasmic cons... more Macroautophagy (mATG) is a major lysosomal mechanism for the bulk degradation of cytoplasmic constituents, including proteins, damaged organelles, and penetrated pathogens. It is characterized by sequestration of entire portions of the cytoplasm by a double-membrane bounded vacuole called autophagosome. Despite its action as a self-digestion process, autophagy is mainly considered to be a well-regulated pathway to provide cell survival and remodeling. A line of evidence indicates that mATG plays a crucial role in both innate and adaptive immunity. It has been shown that mATG can suppress viral infections, inactivate intracellular bacteria and parasites, and deliver cytoplasmic antigens for MHC class II presentation to the adaptive immune system. Thus, the immune system utilizes mATG for degradation of cytoplasmic materials, to both restrict intracellular pathogens and regulate adaptive immunity. Exposure to cytotoxic agents often interferes with this cross-talk, impairs immune syste...

Autophagy - A Double-Edged Sword - Cell Survival or Death?, 2013