Gregory Kintz - Academia.edu (original) (raw)
Papers by Gregory Kintz
IEEE Journal of Quantum Electronics, 1992
The performance of diode bar pumped Nd:YAG and Nd:YLF lasers in a novel tightly folded resonator ... more The performance of diode bar pumped Nd:YAG and Nd:YLF lasers in a novel tightly folded resonator configuration is described. For Nd:YLF at a pump level of 9.2 W a TEM00 output power of 3.1 W with a slope efficiency of 47% was observed. In Q-switched operation a pulse energy of 0.7 mJ and a pulsewidth of 5 ns were obtained.
IEEE Journal of Quantum Electronics, 1990
Single-frequency output power of 35 mW has been observed in 3.0% doped Nd:YVO4 pumped with a 200-... more Single-frequency output power of 35 mW has been observed in 3.0% doped Nd:YVO4 pumped with a 200-mW laser diode. The single-frequency operation is due to the spatial overlap of longitudinal modes at the surface of a mirror in the laser cavity where the material is pumped. The strong absorption coefficient at 808.7 nm and narrow bandwidth of the Nd:YVO4 only
Diode Pumping of Average-Power Solid State Lasers, 1993
Advances of 3-to 10-W average-power diode-pumped lasers. [Proceedings of SPIE 1865, 61 (1993)]. S... more Advances of 3-to 10-W average-power diode-pumped lasers. [Proceedings of SPIE 1865, 61 (1993)]. Sheldon B. Hutchison, Thomas M. Baer, Kelly A. Cox, Phillip H. Gooding, David F. Head, John W. Hobbs, Mark S. Keirstead, Gregory J. Kintz. Abstract. ...
Stereoscopic Displays and Virtual Reality Systems VI, 1999
SID Symposium Digest of Technical Papers, 1999
Cockpit Displays VI: Displays for Defense Applications, 1999
The growing importance of analyzing the human genome to detect hereditary and infectious diseases... more The growing importance of analyzing the human genome to detect hereditary and infectious diseases associated with specific DNA sequences has motivated us to develop automated devices to integrate sample preparation, real-time PCR, and microchannel electrophoresis (MCE). In this report, we present results from an optimized compact system capable of processing a raw sample of blood, extracting the DNA, and performing a multiplexed PCR reaction. Finally, an innovative electrophoretic separation was performed on the post-PCR products using a unique MCE system. The sample preparation system extracted and lysed white blood cells (WBC) from whole blood, producing DNA of sufficient quantity and quality for a polymerase chain reaction (PCR). Separation of multiple amplicons was achieved in a microfabricated channel 30 lm 6 100 lm in cross section and 85 mm in length filled with a replaceable methyl cellulose matrix operated under denaturing conditions at 508C. By incorporating fluorescent-labeled primers in the PCR, the amplicons were identified by a two-color (multiplexed) fluorescence detection system. Two base-pair resolution of single-stranded DNA (PCR products) was achieved. We believe that this integrated system provides a unique solution for DNA analysis.
Journal of Separation Science, 2004
The growing importance of analyzing the human genome to detect hereditary and infectious diseases... more The growing importance of analyzing the human genome to detect hereditary and infectious diseases associated with specific DNA sequences has motivated us to develop automated devices to integrate sample preparation, real-time PCR, and microchannel electrophoresis (MCE). In this report, we present results from an optimized compact system capable of processing a raw sample of blood, extracting the DNA, and performing a multiplexed PCR reaction. Finally, an innovative electrophoretic separation was performed on the post-PCR products using a unique MCE system. The sample preparation system extracted and lysed white blood cells (WBC) from whole blood, producing DNA of sufficient quantity and quality for a polymerase chain reaction (PCR). Separation of multiple amplicons was achieved in a microfabricated channel 30 lm 6 100 lm in cross section and 85 mm in length filled with a replaceable methyl cellulose matrix operated under denaturing conditions at 508C. By incorporating fluorescent-labeled primers in the PCR, the amplicons were identified by a two-color (multiplexed) fluorescence detection system. Two base-pair resolution of single-stranded DNA (PCR products) was achieved. We believe that this integrated system provides a unique solution for DNA analysis.
ELECTROPHORESIS, 2001
A miniature scanning fluorescent detector has been developed for plastic microchannel isoelectric... more A miniature scanning fluorescent detector has been developed for plastic microchannel isoelectric focusing (mIEF) analysis. The detector, comprised of a lamp and photomultiplier tube (PMT) on a moving stage, measured the real-time distribution of fluorescently labeled peptides subjected to gel-free mIEF. During the run, the effective length of the 6-cm channel was scanned every 9 s. Analysis was completed within 5 min while still obtaining high resolution and sensitivity. In addition, the scanning detector was used to characterize peptide migration properties within the channel by providing simultaneous temporal and spatial measurements.
IEEE Journal of Quantum Electronics, 1992
The performance of diode bar pumped Nd:YAG and Nd:YLF lasers in a novel tightly folded resonator ... more The performance of diode bar pumped Nd:YAG and Nd:YLF lasers in a novel tightly folded resonator configuration is described. For Nd:YLF at a pump level of 9.2 W a TEM00 output power of 3.1 W with a slope efficiency of 47% was observed. In Q-switched operation a pulse energy of 0.7 mJ and a pulsewidth of 5 ns were obtained.
IEEE Journal of Quantum Electronics, 1990
Single-frequency output power of 35 mW has been observed in 3.0% doped Nd:YVO4 pumped with a 200-... more Single-frequency output power of 35 mW has been observed in 3.0% doped Nd:YVO4 pumped with a 200-mW laser diode. The single-frequency operation is due to the spatial overlap of longitudinal modes at the surface of a mirror in the laser cavity where the material is pumped. The strong absorption coefficient at 808.7 nm and narrow bandwidth of the Nd:YVO4 only
Diode Pumping of Average-Power Solid State Lasers, 1993
Advances of 3-to 10-W average-power diode-pumped lasers. [Proceedings of SPIE 1865, 61 (1993)]. S... more Advances of 3-to 10-W average-power diode-pumped lasers. [Proceedings of SPIE 1865, 61 (1993)]. Sheldon B. Hutchison, Thomas M. Baer, Kelly A. Cox, Phillip H. Gooding, David F. Head, John W. Hobbs, Mark S. Keirstead, Gregory J. Kintz. Abstract. ...
Stereoscopic Displays and Virtual Reality Systems VI, 1999
SID Symposium Digest of Technical Papers, 1999
Cockpit Displays VI: Displays for Defense Applications, 1999
The growing importance of analyzing the human genome to detect hereditary and infectious diseases... more The growing importance of analyzing the human genome to detect hereditary and infectious diseases associated with specific DNA sequences has motivated us to develop automated devices to integrate sample preparation, real-time PCR, and microchannel electrophoresis (MCE). In this report, we present results from an optimized compact system capable of processing a raw sample of blood, extracting the DNA, and performing a multiplexed PCR reaction. Finally, an innovative electrophoretic separation was performed on the post-PCR products using a unique MCE system. The sample preparation system extracted and lysed white blood cells (WBC) from whole blood, producing DNA of sufficient quantity and quality for a polymerase chain reaction (PCR). Separation of multiple amplicons was achieved in a microfabricated channel 30 lm 6 100 lm in cross section and 85 mm in length filled with a replaceable methyl cellulose matrix operated under denaturing conditions at 508C. By incorporating fluorescent-labeled primers in the PCR, the amplicons were identified by a two-color (multiplexed) fluorescence detection system. Two base-pair resolution of single-stranded DNA (PCR products) was achieved. We believe that this integrated system provides a unique solution for DNA analysis.
Journal of Separation Science, 2004
The growing importance of analyzing the human genome to detect hereditary and infectious diseases... more The growing importance of analyzing the human genome to detect hereditary and infectious diseases associated with specific DNA sequences has motivated us to develop automated devices to integrate sample preparation, real-time PCR, and microchannel electrophoresis (MCE). In this report, we present results from an optimized compact system capable of processing a raw sample of blood, extracting the DNA, and performing a multiplexed PCR reaction. Finally, an innovative electrophoretic separation was performed on the post-PCR products using a unique MCE system. The sample preparation system extracted and lysed white blood cells (WBC) from whole blood, producing DNA of sufficient quantity and quality for a polymerase chain reaction (PCR). Separation of multiple amplicons was achieved in a microfabricated channel 30 lm 6 100 lm in cross section and 85 mm in length filled with a replaceable methyl cellulose matrix operated under denaturing conditions at 508C. By incorporating fluorescent-labeled primers in the PCR, the amplicons were identified by a two-color (multiplexed) fluorescence detection system. Two base-pair resolution of single-stranded DNA (PCR products) was achieved. We believe that this integrated system provides a unique solution for DNA analysis.
ELECTROPHORESIS, 2001
A miniature scanning fluorescent detector has been developed for plastic microchannel isoelectric... more A miniature scanning fluorescent detector has been developed for plastic microchannel isoelectric focusing (mIEF) analysis. The detector, comprised of a lamp and photomultiplier tube (PMT) on a moving stage, measured the real-time distribution of fluorescently labeled peptides subjected to gel-free mIEF. During the run, the effective length of the 6-cm channel was scanned every 9 s. Analysis was completed within 5 min while still obtaining high resolution and sensitivity. In addition, the scanning detector was used to characterize peptide migration properties within the channel by providing simultaneous temporal and spatial measurements.