Anders Grubb - Academia.edu (original) (raw)

Papers by Anders Grubb

Research paper thumbnail of The complexity of kidney disease and diagnosing it – cystatin C, selective glomerular hypofiltration syndromes and proteome regulation

Journal of Internal Medicine

Estimation of kidney function is often part of daily clinical practice, mostly done by using the ... more Estimation of kidney function is often part of daily clinical practice, mostly done by using the endogenous glomerular filtration rate (GFR)‐markers creatinine or cystatin C. A recommendation to use both markers in parallel in 2010 has resulted in new knowledge concerning the pathophysiology of kidney disorders by the identification of a new set of kidney disorders, selective glomerular hypofiltration syndromes. These syndromes, connected to strong increases in mortality and morbidity, are characterized by a selective reduction in the glomerular filtration of 5–30 kDa molecules, such as cystatin C, compared to the filtration of small molecules <1 kDa dominating the glomerular filtrate, for example water, urea and creatinine. At least two types of such disorders, shrunken or elongated pore syndrome, are possible according to the pore model for glomerular filtration. Selective glomerular hypofiltration syndromes are prevalent in investigated populations, and patients with these syn...

Research paper thumbnail of Immunoassay with test strip having antibodies bound thereto

Research paper thumbnail of Taupin P, Jasodhara R, Fischer WH, Suhr ST, Hakansson K, Grubb A, Gage FH: FGF-2-responsive neural stem cell proliferation requires CCg (glycosylated cystatin C), a novel autocrine/paracrine cofactor. (2000) Neuron 28: 385-397

Research paper thumbnail of Janowski R, Abrahamson M, Grubb A, Jaskolski M: Domain swapping in N-truncated human cystatin C. (2004) J Mol Biol 341: 151-160

Journal of Molecular Biology

Research paper thumbnail of Brand H, Lerner UH, Grubb A, Beertsen W, Nieuw Amerongen AV, Everts V: Family 2 cystatins inhibit osteoclast-mediated bone resorption in calvarial bone explants (2004) Bone 35: 689-696

Research paper thumbnail of Kaeser SA, Herzig MC, Coomaraswamy J, Kilger E, Selenica ML, Winkler DT, Staufenbiel M, Levy E, Grubb A, Jucker M: Cystatin C modulates cerebral beta-amyloidosis. (2007) Nat Genet 39: 1437-1439

Research paper thumbnail of Kaur G, Mohan P, Pawlik M, DeRosa S, Fajiculay J, Che S, Grubb A, Ginsberg SD, Nixon RA, Levy E: Cystatin C rescues degenerating neurons in a cystatin B-knockout mouse model of progressive myoclonus epilepsy. (2010) Am J Pathol 177: 2256-2267

American Journal Of Pathology

Research paper thumbnail of Tizon B, Sahoo S, Yu H, Gauthier S, Kumar AR, Mohan P, Pawlik M, Figliola M, Pawlik P, Grubb A, Uchiyama Y, Bandyopadhyay U, Cuervo AM, Nixon RA, Levy E: Induction of autophagy by cystatin C: A mechanism that protects murine primary cortical neurons and neuronal cell lines. (2010) PLoS One 5(3): ...

Tizon B, Sahoo S, Yu H, Gauthier S, Kumar AR, Mohan P, Pawlik M, Figliola M, Pawlik P, Grubb A, Uchiyama Y, Bandyopadhyay U, Cuervo AM, Nixon RA, Levy E: Induction of autophagy by cystatin C: A mechanism that protects murine primary cortical neurons and neuronal cell lines. (2010) PLoS One 5(3): ...

PLoS ONE

Research paper thumbnail of Grubb A, Christensson A. U-Albumin-Kreatinin-kvot och estimerat GFR (eGFR), baserat på medelvärdet av eGFRcystatin C och eGFRkreatinin, (eGFRmedel), är de bästa måtten för att diagnosticera och följa njursjukdom. (2013) Läkartidningen 110: 1021-1024

Research paper thumbnail of Immunofluorescent Demonstration of the Presence of Protein HC on the Surface of Human Lymphocytes

Acta Medica Scandinavica, 2009

Indirect immunofluorescence was used to demonstrate the presence of protein HC on the surface of ... more Indirect immunofluorescence was used to demonstrate the presence of protein HC on the surface of a large percentage of normal human peripheral blood lymphocytes. Protein HC is a recently described charge heterogeneous, complex‐forming glycoprotein normally present in human plasma, urine and cerebrospinal fluid. The synthesis of protein HC by lymphocytes was indicated by the removal of the glycoprotein from the cell surfaces on trypsinization of the cells followed by the reappearance of the protein on continued cultivation of the cells.

Research paper thumbnail of Quantitation of J Chain in Human Biological Fluids by a Simple Immunochemical Procedure

Acta Medica Scandinavica, 2009

ABSTRACT. The molecular form and immunochemical properties of the J chain populations released on... more ABSTRACT. The molecular form and immunochemical properties of the J chain populations released on reduction and carboxymethylation of normal human plasma, milk, saliva and of plasma containing IgA or IgM M‐components were investigated. A procedure was devised to release the entire J chain population from these various sources and to produce immunochemically identical J chain populations containing only J chain monomers. AH identical standard J chain population was purified and quantitated by physiochemical means. A specific rabbit anti‐J chain antiserum was raised against this pure J chain population. A simple and rapid immunochemical method for J chain quantitation in complex biological fluids as well as in solutions of pure polymeric immunoglobulins was constructed on these grounds. The J chain concentration was found to be (mean +S.D.) 1.74+0.65 μM in normal human plasma, 1.94+1.21 μM. in human milk and 0.48+0.26 +M in human saliva. The J chain/IgA molar ratio was found to be (me...

Research paper thumbnail of Estimating GFR prior to contrast medium examinations-what the radiologist needs to know!

European radiology, Jan 28, 2015

Creatinine-based equations to estimate glomerular filtration rate (GFR) are increasingly used in ... more Creatinine-based equations to estimate glomerular filtration rate (GFR) are increasingly used in radiological practice and in studies on contrast medium-induced acute kidney injury (CIAKI). Their use is recommended in guidelines and contrast medium textbooks to identify patients at risk of CIAKI or nephrogenic systemic fibrosis. There is also an increased interest in cystatin C-based equations. Adopting GFR equations requires local creatinine and cystatin C assay calibrations to equal those used in developing the equations to avoid overestimation or underestimation of renal function. Methods should preferably be traceable to international standards, and assay traceability should be defined in CIAKI studies. Absolute GFR (mL/min) should be used when dosing contrast media and relating the dose to CIAKI instead of commonly used relative GFR (mL/min/1.73 m(2)) estimates. Accuracy of creatinine and cystatin C equations (percentage of GFR estimates within 30 % of measured GFR) ranges betw...

Research paper thumbnail of Serum cystatin C: a replacement for creatinine as a biochemical marker of GFR

Kidney international. Supplement, 1994

Search by Subject Search using Medical Subject Headings (< b> MeSH</b>), a controlled... more Search by Subject Search using Medical Subject Headings (< b> MeSH</b>), a controlled vocabulary for indexing life sciences content.< br/> Note that some records do not have MeSH. These include Patents and the latest PubMed and PubMed Central records.

Research paper thumbnail of AA-amyloidosis. Tissue component-specific association of various protein AA subspecies and evidence of a fourth SAA gene product

The American journal of pathology, 1990

Protein AA, the major repetitive protein subunit present in fibrils deposited in AA-amyloidosis, ... more Protein AA, the major repetitive protein subunit present in fibrils deposited in AA-amyloidosis, is an N-terminal cleavage product of a 104-amino acid precursor, serum amyloid A (SAA). Protein AA subspecies varying between 45 and 94 amino acids in length have been described. In this study it is shown that the different protein AA subspecies are not evenly distributed in amyloid deposits and that in single patients, certain subspecies of protein AA are deposited in specific tissue component sites. Thus larger protein AA subspecies occur in lower concentration in amyloid in the glomeruli compared to other sites and are especially found in amyloid in vessel walls. Three different SAA forms have been predicted from genomic and complementary DNA studies. The existence of a fourth type has been suspected from amino acid sequence studies of purified SAA. Protein AA derived from this fourth type of SAA is now shown to be present in amyloid fibrils in one of the patients studied in this paper.

Research paper thumbnail of Low Resolution Structure in Solution of Native Human Cystamin C

Research paper thumbnail of Renal Tubular Dysfunction After Urinary Diversion

Scandinavian Journal of Urology and Nephrology, 1995

ABSTRACT

Research paper thumbnail of Long-term Stability of Albumin, Protein HC, Immunoglobulin G, κ- and λ-chain-immunoreactivity, Orosomucoid and α1-antitrypsin in Urine Stored at -20°C

Scandinavian Journal of Urology and Nephrology, 1997

The stability of albumin, protein HC, immunoglobulin G, kappa- and lambda-chain immunoreactivity,... more The stability of albumin, protein HC, immunoglobulin G, kappa- and lambda-chain immunoreactivity, orosomucoid and alpha 1-antitrypsin in urine stored at -20 degrees C for up to 24 months was investigated. Significant decreases of the median concentration values for protein HC, IgG and alpha 1-antitrypsin were observed for native urine. Addition to urine of a preservative solution containing benzamidinium chloride, EDTA, tris(hydroxymethyl)-aminomethane and azide prevented the decreases of the concentration values for protein HC and IgG but not for alpha 1-antitrypsin. The median concentration values for albumin, orosomucoid and kappa- and lambda-chain immunoreactivity did not change significantly upon storage of native urine, nor for urine with the preservative solution.

Research paper thumbnail of Different equations to combine creatinine and cystatin C to predict GFR. Arithmetic mean of existing equations performs as well as complex combinations

Scandinavian Journal of Clinical and Laboratory Investigation, 2009

To test various ways of combining creatinine and cystatin C in equations to predict glomerular fi... more To test various ways of combining creatinine and cystatin C in equations to predict glomerular filtration rate (GFR). Performance of the following expressions to predict GFR was compared with measured GFR (iohexol clearance, mL/min/1.73 m(2)) in 857 patients: (i) Lund-Malmö creatinine equation, (ii) Grubb cystatin C equation, (iii) arithmetic mean of (1) and (2), (iv) geometric mean of (1) and (2), (v) linear regression on (1) and (2), (vi) regression on (1) and cystatin C, and (vii) regression on creatinine, cystatin C, age and gender. For the entire cohort median percent error (bias) was &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;5% for all expressions, though all expressions tended to underestimate (-8.3 to 15.8%) GFR at levels &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt; or =90 mL/min/1.73 m(2). The five expressions combining creatinine and cystatin C significantly improved correlation and accuracy (p&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.001) within 15 and 30% of measured GFR compared with the equations based on the separate analytes and with no significant difference between the five expressions. In a subgroup of patients with neurological disease and muscle atrophy the cystatin C equation performed better than the expressions combining creatinine and cystatin C. Simply calculating the arithmetic mean of predicted GFR based on separate creatinine and cystatin C equations performs equally well as more complex equations. Reporting GFR based on separate creatinine and cystatin C equations, and their arithmetic mean also has the definite advantage that the physician can choose the estimated GFR, most appropriate depending on the clinical setting and patient characteristics.

Research paper thumbnail of Analysis of the Immunochemical Relationship between Antigens by Electrophoresis in Agarose Gels Containing Antibodies

Scandinavian Journal of Clinical and Laboratory Investigation, 1972

... 6. 7. 8. Grubb, A. Quantitative determination of the distribution of the specific antibodies ... more ... 6. 7. 8. Grubb, A. Quantitative determination of the distribution of the specific antibodies of anti-sera subjected to gel electrophoresis. Accepted for publication in Immunochemistry. ... Invest. 24, 55, 1969. Laurel], C.-B. Purification and properties of different haptoglobins. Clin. Chim. ...

Research paper thumbnail of Quantitation of Immunoglobulin G by Electrophoresis in Agarose Gel Containing Antibodies

Scandinavian Journal of Clinical and Laboratory Investigation, 1970

... Grubb, A. Quantitation of Immunoglobulin G by Electrophoresis in Agarose Gel Containing Antib... more ... Grubb, A. Quantitation of Immunoglobulin G by Electrophoresis in Agarose Gel Containing Antibodies. Scand. J. clin. Lab. Invest. 26, 249-255, 1970. It is shown that the method of protein quantitation by electrophoresis in antibody-containing agarose introduced by Laurell in ...

Research paper thumbnail of The complexity of kidney disease and diagnosing it – cystatin C, selective glomerular hypofiltration syndromes and proteome regulation

Journal of Internal Medicine

Estimation of kidney function is often part of daily clinical practice, mostly done by using the ... more Estimation of kidney function is often part of daily clinical practice, mostly done by using the endogenous glomerular filtration rate (GFR)‐markers creatinine or cystatin C. A recommendation to use both markers in parallel in 2010 has resulted in new knowledge concerning the pathophysiology of kidney disorders by the identification of a new set of kidney disorders, selective glomerular hypofiltration syndromes. These syndromes, connected to strong increases in mortality and morbidity, are characterized by a selective reduction in the glomerular filtration of 5–30 kDa molecules, such as cystatin C, compared to the filtration of small molecules <1 kDa dominating the glomerular filtrate, for example water, urea and creatinine. At least two types of such disorders, shrunken or elongated pore syndrome, are possible according to the pore model for glomerular filtration. Selective glomerular hypofiltration syndromes are prevalent in investigated populations, and patients with these syn...

Research paper thumbnail of Immunoassay with test strip having antibodies bound thereto

Research paper thumbnail of Taupin P, Jasodhara R, Fischer WH, Suhr ST, Hakansson K, Grubb A, Gage FH: FGF-2-responsive neural stem cell proliferation requires CCg (glycosylated cystatin C), a novel autocrine/paracrine cofactor. (2000) Neuron 28: 385-397

Research paper thumbnail of Janowski R, Abrahamson M, Grubb A, Jaskolski M: Domain swapping in N-truncated human cystatin C. (2004) J Mol Biol 341: 151-160

Journal of Molecular Biology

Research paper thumbnail of Brand H, Lerner UH, Grubb A, Beertsen W, Nieuw Amerongen AV, Everts V: Family 2 cystatins inhibit osteoclast-mediated bone resorption in calvarial bone explants (2004) Bone 35: 689-696

Research paper thumbnail of Kaeser SA, Herzig MC, Coomaraswamy J, Kilger E, Selenica ML, Winkler DT, Staufenbiel M, Levy E, Grubb A, Jucker M: Cystatin C modulates cerebral beta-amyloidosis. (2007) Nat Genet 39: 1437-1439

Research paper thumbnail of Kaur G, Mohan P, Pawlik M, DeRosa S, Fajiculay J, Che S, Grubb A, Ginsberg SD, Nixon RA, Levy E: Cystatin C rescues degenerating neurons in a cystatin B-knockout mouse model of progressive myoclonus epilepsy. (2010) Am J Pathol 177: 2256-2267

American Journal Of Pathology

Research paper thumbnail of Tizon B, Sahoo S, Yu H, Gauthier S, Kumar AR, Mohan P, Pawlik M, Figliola M, Pawlik P, Grubb A, Uchiyama Y, Bandyopadhyay U, Cuervo AM, Nixon RA, Levy E: Induction of autophagy by cystatin C: A mechanism that protects murine primary cortical neurons and neuronal cell lines. (2010) PLoS One 5(3): ...

Tizon B, Sahoo S, Yu H, Gauthier S, Kumar AR, Mohan P, Pawlik M, Figliola M, Pawlik P, Grubb A, Uchiyama Y, Bandyopadhyay U, Cuervo AM, Nixon RA, Levy E: Induction of autophagy by cystatin C: A mechanism that protects murine primary cortical neurons and neuronal cell lines. (2010) PLoS One 5(3): ...

PLoS ONE

Research paper thumbnail of Grubb A, Christensson A. U-Albumin-Kreatinin-kvot och estimerat GFR (eGFR), baserat på medelvärdet av eGFRcystatin C och eGFRkreatinin, (eGFRmedel), är de bästa måtten för att diagnosticera och följa njursjukdom. (2013) Läkartidningen 110: 1021-1024

Research paper thumbnail of Immunofluorescent Demonstration of the Presence of Protein HC on the Surface of Human Lymphocytes

Acta Medica Scandinavica, 2009

Indirect immunofluorescence was used to demonstrate the presence of protein HC on the surface of ... more Indirect immunofluorescence was used to demonstrate the presence of protein HC on the surface of a large percentage of normal human peripheral blood lymphocytes. Protein HC is a recently described charge heterogeneous, complex‐forming glycoprotein normally present in human plasma, urine and cerebrospinal fluid. The synthesis of protein HC by lymphocytes was indicated by the removal of the glycoprotein from the cell surfaces on trypsinization of the cells followed by the reappearance of the protein on continued cultivation of the cells.

Research paper thumbnail of Quantitation of J Chain in Human Biological Fluids by a Simple Immunochemical Procedure

Acta Medica Scandinavica, 2009

ABSTRACT. The molecular form and immunochemical properties of the J chain populations released on... more ABSTRACT. The molecular form and immunochemical properties of the J chain populations released on reduction and carboxymethylation of normal human plasma, milk, saliva and of plasma containing IgA or IgM M‐components were investigated. A procedure was devised to release the entire J chain population from these various sources and to produce immunochemically identical J chain populations containing only J chain monomers. AH identical standard J chain population was purified and quantitated by physiochemical means. A specific rabbit anti‐J chain antiserum was raised against this pure J chain population. A simple and rapid immunochemical method for J chain quantitation in complex biological fluids as well as in solutions of pure polymeric immunoglobulins was constructed on these grounds. The J chain concentration was found to be (mean +S.D.) 1.74+0.65 μM in normal human plasma, 1.94+1.21 μM. in human milk and 0.48+0.26 +M in human saliva. The J chain/IgA molar ratio was found to be (me...

Research paper thumbnail of Estimating GFR prior to contrast medium examinations-what the radiologist needs to know!

European radiology, Jan 28, 2015

Creatinine-based equations to estimate glomerular filtration rate (GFR) are increasingly used in ... more Creatinine-based equations to estimate glomerular filtration rate (GFR) are increasingly used in radiological practice and in studies on contrast medium-induced acute kidney injury (CIAKI). Their use is recommended in guidelines and contrast medium textbooks to identify patients at risk of CIAKI or nephrogenic systemic fibrosis. There is also an increased interest in cystatin C-based equations. Adopting GFR equations requires local creatinine and cystatin C assay calibrations to equal those used in developing the equations to avoid overestimation or underestimation of renal function. Methods should preferably be traceable to international standards, and assay traceability should be defined in CIAKI studies. Absolute GFR (mL/min) should be used when dosing contrast media and relating the dose to CIAKI instead of commonly used relative GFR (mL/min/1.73 m(2)) estimates. Accuracy of creatinine and cystatin C equations (percentage of GFR estimates within 30 % of measured GFR) ranges betw...

Research paper thumbnail of Serum cystatin C: a replacement for creatinine as a biochemical marker of GFR

Kidney international. Supplement, 1994

Search by Subject Search using Medical Subject Headings (< b> MeSH</b>), a controlled... more Search by Subject Search using Medical Subject Headings (< b> MeSH</b>), a controlled vocabulary for indexing life sciences content.< br/> Note that some records do not have MeSH. These include Patents and the latest PubMed and PubMed Central records.

Research paper thumbnail of AA-amyloidosis. Tissue component-specific association of various protein AA subspecies and evidence of a fourth SAA gene product

The American journal of pathology, 1990

Protein AA, the major repetitive protein subunit present in fibrils deposited in AA-amyloidosis, ... more Protein AA, the major repetitive protein subunit present in fibrils deposited in AA-amyloidosis, is an N-terminal cleavage product of a 104-amino acid precursor, serum amyloid A (SAA). Protein AA subspecies varying between 45 and 94 amino acids in length have been described. In this study it is shown that the different protein AA subspecies are not evenly distributed in amyloid deposits and that in single patients, certain subspecies of protein AA are deposited in specific tissue component sites. Thus larger protein AA subspecies occur in lower concentration in amyloid in the glomeruli compared to other sites and are especially found in amyloid in vessel walls. Three different SAA forms have been predicted from genomic and complementary DNA studies. The existence of a fourth type has been suspected from amino acid sequence studies of purified SAA. Protein AA derived from this fourth type of SAA is now shown to be present in amyloid fibrils in one of the patients studied in this paper.

Research paper thumbnail of Low Resolution Structure in Solution of Native Human Cystamin C

Research paper thumbnail of Renal Tubular Dysfunction After Urinary Diversion

Scandinavian Journal of Urology and Nephrology, 1995

ABSTRACT

Research paper thumbnail of Long-term Stability of Albumin, Protein HC, Immunoglobulin G, κ- and λ-chain-immunoreactivity, Orosomucoid and α1-antitrypsin in Urine Stored at -20°C

Scandinavian Journal of Urology and Nephrology, 1997

The stability of albumin, protein HC, immunoglobulin G, kappa- and lambda-chain immunoreactivity,... more The stability of albumin, protein HC, immunoglobulin G, kappa- and lambda-chain immunoreactivity, orosomucoid and alpha 1-antitrypsin in urine stored at -20 degrees C for up to 24 months was investigated. Significant decreases of the median concentration values for protein HC, IgG and alpha 1-antitrypsin were observed for native urine. Addition to urine of a preservative solution containing benzamidinium chloride, EDTA, tris(hydroxymethyl)-aminomethane and azide prevented the decreases of the concentration values for protein HC and IgG but not for alpha 1-antitrypsin. The median concentration values for albumin, orosomucoid and kappa- and lambda-chain immunoreactivity did not change significantly upon storage of native urine, nor for urine with the preservative solution.

Research paper thumbnail of Different equations to combine creatinine and cystatin C to predict GFR. Arithmetic mean of existing equations performs as well as complex combinations

Scandinavian Journal of Clinical and Laboratory Investigation, 2009

To test various ways of combining creatinine and cystatin C in equations to predict glomerular fi... more To test various ways of combining creatinine and cystatin C in equations to predict glomerular filtration rate (GFR). Performance of the following expressions to predict GFR was compared with measured GFR (iohexol clearance, mL/min/1.73 m(2)) in 857 patients: (i) Lund-Malmö creatinine equation, (ii) Grubb cystatin C equation, (iii) arithmetic mean of (1) and (2), (iv) geometric mean of (1) and (2), (v) linear regression on (1) and (2), (vi) regression on (1) and cystatin C, and (vii) regression on creatinine, cystatin C, age and gender. For the entire cohort median percent error (bias) was &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;5% for all expressions, though all expressions tended to underestimate (-8.3 to 15.8%) GFR at levels &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt; or =90 mL/min/1.73 m(2). The five expressions combining creatinine and cystatin C significantly improved correlation and accuracy (p&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.001) within 15 and 30% of measured GFR compared with the equations based on the separate analytes and with no significant difference between the five expressions. In a subgroup of patients with neurological disease and muscle atrophy the cystatin C equation performed better than the expressions combining creatinine and cystatin C. Simply calculating the arithmetic mean of predicted GFR based on separate creatinine and cystatin C equations performs equally well as more complex equations. Reporting GFR based on separate creatinine and cystatin C equations, and their arithmetic mean also has the definite advantage that the physician can choose the estimated GFR, most appropriate depending on the clinical setting and patient characteristics.

Research paper thumbnail of Analysis of the Immunochemical Relationship between Antigens by Electrophoresis in Agarose Gels Containing Antibodies

Scandinavian Journal of Clinical and Laboratory Investigation, 1972

... 6. 7. 8. Grubb, A. Quantitative determination of the distribution of the specific antibodies ... more ... 6. 7. 8. Grubb, A. Quantitative determination of the distribution of the specific antibodies of anti-sera subjected to gel electrophoresis. Accepted for publication in Immunochemistry. ... Invest. 24, 55, 1969. Laurel], C.-B. Purification and properties of different haptoglobins. Clin. Chim. ...

Research paper thumbnail of Quantitation of Immunoglobulin G by Electrophoresis in Agarose Gel Containing Antibodies

Scandinavian Journal of Clinical and Laboratory Investigation, 1970

... Grubb, A. Quantitation of Immunoglobulin G by Electrophoresis in Agarose Gel Containing Antib... more ... Grubb, A. Quantitation of Immunoglobulin G by Electrophoresis in Agarose Gel Containing Antibodies. Scand. J. clin. Lab. Invest. 26, 249-255, 1970. It is shown that the method of protein quantitation by electrophoresis in antibody-containing agarose introduced by Laurell in ...