Gubio Campos - Academia.edu (original) (raw)

Papers by Gubio Campos

Brazilian Journal of Development

This study aimed to describe the neurodevelopmental of children born to mothers exposed to Zika v... more This study aimed to describe the neurodevelopmental of children born to mothers exposed to Zika virus (ZIKV) without birth defects. Nine children aged 20 to 46 months were assessed by the Bayley III Scales. Of these, one child had tested positive ZIKV-IgG, whilst the others had negative results. All children underwent neuropediatric evaluation and some had magnetic resonance imaging. Six children had developmental changes (language delay was observed in all of them); four with Autism Spectrum Disorder and two motor and language delay. Three children obtained satisfactory results in Bayley-III. The results shed light on the hypothesis that cognitive changes observed in five children maybe related to exposure to the effects of ZIKV infection on the maternal environment.

Arquivos De Gastroenterologia, Sep 1, 2018

Background-Norovirus (NoV) is an important etiologic agent of acute gastroenteritis and infects i... more Background-Norovirus (NoV) is an important etiologic agent of acute gastroenteritis and infects individuals of all ages, especially children in Brazil and worldwide. NoV GII.4 was the most prevalent genotype worldwide because of your extensive genetic diversity. In Brazil, especially in the Northeast, few studies have been developed for identify and molecularly characterize NoV. Objective-The present study aimed to detect and describe the molecular epidemiology of NoV associated with acute gastroenteritis. Methods-The viral RNA extracted from stool samples were subjected to Nested RT-PCR and the genotypes were determined by nucleotide sequences analysis. In total, 278 stool samples assisted at Aliança Hospital in the city of Salvador, with acute gastroenteritis were examined, between March 2009 and July 2012. Results-A high NoV rate (54.2%) was identified in children under 5 years of age. We detected the circulation of different NoV GII.4 variants in Salvador, during the study period as Den Haag 2006b, New Orleans 2009 and Sydney 2012. Conclusion-These findings reinforce the need to study the molecular epidemiology of NoV infections in acute gastroenteritis. HEADINGS-Norovirus. Gastroenteritis. Phylogeny.

Brazilian Journal of Infectious Diseases, Feb 1, 2007

Rotavirus is a major cause of infectious diarrhea in infants and young children. The objective of... more Rotavirus is a major cause of infectious diarrhea in infants and young children. The objective of this study was to characterize the genotypes of Human Rotavirus found in children hospitalized with acute diarrhea in the Pediatric Hospital Prof. Hosannah de Oliveira of the UFBA in Salvador, Bahia, Brazil, during the years of 1999, 2000 and 2002. Fecal samples were analyzed (n=358) by methods EIARA and SDS-PAGE for detection of Rotavirus. Positive samples of one or two of these methods (n=168) were submitted to RT-PCR and Multiplex-Nested PCR to determine genotypes G and P. A hundred sixty-eight (46.9%) samples were positive and 190 (53.1%) negative. Only 17 (4.7%) samples had divergent results. The distribution of genotypes G during the first year, showed that the genotype G9 was present in 96,8% of the analyzed samples, in the second year, it was responsible for 96% and in the third year, 88,1%. The characterization of genotypes P demonstrated that the genotype P1A[8] was the most outstanding in all years. In this study we discuss the benefit to control the genotypes of Rotavirus through the molecular characterization for the development of potential vaccines.

Journal of Clinical Microbiology, Aug 1, 2005

Two hundred eight of 648 (32%) diarrheal stool samples collected from hospitalized children under... more Two hundred eight of 648 (32%) diarrheal stool samples collected from hospitalized children under 5 years of age during a 3-year period (1999, 2000, and 2002) in the city of Salvador, in the state of Bahia, Brazil, were rotavirus positive. One hundred sixty-four of 208 (78.8%) rotavirus-positive samples had genotype G9 specificity, predominantly in association with P[8]. Other specificities detected were G1 (12.0%) and G4 (1.4%). Viruses with G2, G3, or P[4] specificity were not detected. Rotavirus genotype G9 predominated during each of the three seasons studied; it represented 89.2% of rotavirus strains detected in 1999, 85.3% in 2000, and 74.5% in 2002. G1 viruses (the globally most common G type) have a unique epidemiological characteristic of maintaining predominance during multiple consecutive rotavirus seasons. We have shown in this study for the first time that the G9 viruses also have a similar epidemiological characteristic, albeit for a shorter period of surveillance. The next generation of rotavirus vaccines will need to provide adequate protection against disease caused by G9 viruses.

Archives of Virology, Apr 8, 2008

The molecular epidemiology of a recent norovirus (NoV) outbreak in Brazil performed by comparativ... more The molecular epidemiology of a recent norovirus (NoV) outbreak in Brazil performed by comparative analysis with Genebank NoV sequences showed that the GII.4 strain was responsible for 72.5% of all NoV-positive cases (58/80). Other detected NoV strains included GII.3 (7/80; 8.8%) and GII.9 (8/80; 10%). This is the first outbreak reported in Bahia state, Brazil, during June-July of 2006, where NoV was identified as the principal etiologic agent in hospitalized young adults with acute gastroenteritis symptoms. These findings suggest that GII.4 is a predominant circulating genotype in NoV outbreaks in Brazil.

PLOS ONE, Mar 6, 2019

Background Rapid diagnosis tests (RDTs) are easy to carry out, provide fast results, and could po... more Background Rapid diagnosis tests (RDTs) are easy to carry out, provide fast results, and could potentially guide medical treatment decisions. We investigated the performance of a commercially available RDT, which simultaneously detects the non-structural 1 (NS1) dengue virus (DENV) antigen, and IgM and IgG DENV antibodies, using representative serum samples from individuals in a dengue endemic area in Salvador, Brazil. Methodology/Principal findings We evaluated the accuracy of the SD BIOLINE Dengue Duo RDT (Abbott, Santa Clara, USA; former Alere Inc, Waltham, USA) in a random collection of sera. Samples included acute-phase sera from 246 laboratory-confirmed dengue cases and 108 non-dengue febrile patients enrolled in a surveillance study for dengue detection, 73 healthy controls living in the same surveillance community, and 73 blood donors. RDT accuracy was blindly assessed based on the combined results for the NS1 and the IgM test components. The RDT sensitivity was 46.8% (38.6% for the NS1 component and 13.8% for the IgM component). Sensitivity was greater for samples obtained from patients with secondary DENV infections (49.8%) compared to primary infections (31.1%) (P: 0.02) and was also influenced by the result in the confirmatory dengue diagnostic test, ranging from 39.7% for samples of cases confirmed by IgM-ELISA seroconversion between paired samples to 90.4% for samples of cases confirmed by a positive NS1-ELISA. The RDT specificity was 94.4% for nondengue febrile patients, 87.7% for the community healthy controls, and 95.9% for the blood donors.

IDCases, 2016

To report the first case of Chikungunya encephalitis acquired in the perinatal period during the ... more To report the first case of Chikungunya encephalitis acquired in the perinatal period during the current outbreak in Brazil Methods: Case report. Results: A male neonate with 3500 g developed macular erythematous rash, hypoactivity and fever progressing to generalized seizures. His mother had experienced a disseminated rash and fever before delivery. EEG showed diffuse slowing and cranial NMR was suggestive of encephalitis. Rt-PCR for Chikungunya virus (CHIKV) was positive in cerebrospinal fluid (CSF), blood, urine and saliva. The newborn was discharged home with neurological improvement. Conclusion: We report the first case of a perinatal CHIKV infection associated with a rapidly evolving encephalitis and an extensive dissemination of the virus as documented by positive rt-PCR results in CSF, blood, urine and saliva in the present outbreak in Brazil. In countries experiencing outbreaks of CHIKV infections, clinicians and neonatologists must be familiar with the possibility of the occurrence of neurologic complications and its possible consequences.

Emerging Infectious Diseases, Dec 1, 2015

treated with doxycycline (100 mg 2×/d) for 10 days; they clinically recovered, and laboratory abn... more treated with doxycycline (100 mg 2×/d) for 10 days; they clinically recovered, and laboratory abnormalities returned to reference ranges at discharge 12 days after admission. The remaining 22 persons did not report any malaise and were examined 1 month after tick bite. They appeared healthy at that time, and PCR and ELISA gave negative results, arguing against possible asymptomatic B. miyamotoi infection. We estimated the probability of B. miyamotoi transmission to humans to be 8.3% (95% CI 4%-18% using a Bayesian approach [8] or 95% CI 0%-21% using an SPSS bootstrapping procedure [SPSS Inc., Chicago, IL, USA]). For comparison, among 68 persons bitten by B. burgdorferi sensu lato-infected ticks in the Netherlands, erythema migrans developed in 4.4% (95% C.I. 2.1%-8.3%) persons; 3 (4.4%) others seroconverted without clinical symptoms (9). This pilot study has several limitations. We did not follow up persons bitten by B. burgdorferi sensu lato-or TBEV-infected ticks because they received either antimicrobial drugs or anti-TBE immunoglobulin as a preventive measure. Because of labor constrains, we did not study persons bitten by "PCR-uninfected" ticks; however, they were not hospitalized at RHID, the only hospital in the region for patients with evident tick-borne diseases. We did not use any serologic techniques specific for relapsing fever Borreli (e.g., GlpQ ELISA). Although we did not test for Rickettsia or Babesia spp., we did not find TBEV RNA, B. burgdorferi sensu lato 16S RNA, pathogenic Ehrlichia 16S RNA, or pathogenic Anaplasma DNA in the 2 B. miyamotoi-positive patients' blood samples. We demonstrated that the transmission rate of B. miyamotoi appears to be equal to, or higher, than that of B. burgdorferi sensu lato (1,9,10). Our data indicate that, annually, clinical B. miyamotoi infection might develop in at least 0.005% of persons living in regions to which Ixodes spp. ticks and B. miyamotoi are endemic. This estimate corresponds to ≈33 cases annually in Izhevsk, which is similar to the previously published results of hospital-based surveillance for B. miyamotoi (3,6).

Revista de Ciências Médicas e Biológicas, Jan 18, 2006

Resumo O vírus da Artrite encefalite caprina é o agente causal de uma doença progressiva e debili... more Resumo O vírus da Artrite encefalite caprina é o agente causal de uma doença progressiva e debilitante em caprinos, em que as células da linhagem monócito/macrófago são as principais hospedeiras do vírus in vivo. Essas células estão presentes no colostro, no leite ou no sangue. Estudos demonstram que a replicação viral é dependente do nível de maturação ou diferenciação da célula monocítica, influenciando na sensibilidade de detecção do vírus. Neste estudo, utilizamos o cocultivo de monócitos/macrófagos com células de membrana sinovial de cabras, para o isolamento do vírus circulante no campo; a técnica de double nested PCR (dn PCR) dos co-cultivos e sangue, viabilizou a confirmação do isolamento viral, e a detecção direta do vírus no sangue. Através dessa técnica, detectou-se a presença do DNA proviral em animais soronegativos por Imunodifusão em Gel de Agarose (IDGA); esses achados confirmam que o tempo entre o inicio da infecção e o aparecimento de anticorpos no sangue é variável, facilitando a permanência de animais falsos-negativos no rebanho. Nas amostras processadas, tivemos uma divergência de resultados entre amostras sorologicamente positivas, entretanto, negativas por dn PCR, quando se utilizou a amplificação direta do sangue. Quanto ao cultivo de monócitos/ macrófagos in vitro e posterior co-cultivo com células de MSC obteve-se êxito pelo isolamento do vírus em quatro animais, havendo sido um deles soronegativo. O presente estudo demonstra o primeiro isolamento do vírus nos rebanhos do estado da Bahia, além da implementação da técnica de dn PCR, em co-cultivo de células.

Japanese Journal of Infectious Diseases, 2020

Journal of Clinical Microbiology, Sep 1, 2016

Metagenomic next-generation sequencing (mNGS) of samples from 15 patients with documented Zika vi... more Metagenomic next-generation sequencing (mNGS) of samples from 15 patients with documented Zika virus (ZIKV) infection in Bahia, Brazil, from April 2015 to January 2016 identified coinfections with chikungunya virus (CHIKV) in 2 of 15 ZIKV-positive cases by PCR (13.3%). While generally nonspecific, the clinical presentation corresponding to these two CHIKV/ZIKV coinfections reflected infection by the virus present at a higher titer. Aside from CHIKV and ZIKV, coinfections of other viral pathogens were not detected. The mNGS approach is promising for differential diagnosis of acute febrile illness and identification of coinfections, although targeted arbovirus screening may be sufficient in the current ZIKV outbreak setting.

Revista de Ciências Médicas e Biológicas, Sep 24, 2012

Detecção precoce de caprinos infectados com Lentivirus de pequenos ruminantes através do ensaio E... more Detecção precoce de caprinos infectados com Lentivirus de pequenos ruminantes através do ensaio ELISA.

Epidemiology and Infection, Feb 23, 2015

Dengue virus, commonly transmitted by mosquitoes, causes a human disease of significant social im... more Dengue virus, commonly transmitted by mosquitoes, causes a human disease of significant social impact and presents a serious public health problem in Brazil. This report describes the unusual emergence of DENV-4 in northern Brazil after a nearly 30-year-long absence. DENV-4 genotype I is of Asian origin and was identified in the serum of patients receiving treatment at a hospital serving the Salvador area (Brazilian state of Bahia). The identification of dengue virus serotypes through molecular and phylogenetic analysis is essential for predicting disease severity or fatal illness, principally in endemic countries such as Brazil.

Pubvet, Jul 1, 2011

Amostras de sangue foram coletadas de reprodutoras vacinadas ou sem vacinar para determinar os ní... more Amostras de sangue foram coletadas de reprodutoras vacinadas ou sem vacinar para determinar os níveis de anticorpos contra o PPV através da técnica de inibição da hemaglutinação (HI). Os resultados mostraram que 94,12% (208/221) do total das reprodutoras em estudo tinham títulos protetores de anticorpos (≥ 1:80). Nas fêmeas de rebanhos sem vacinar os títulos HI foram desde 1:195 até 1: 48.488. Nas fazendas que vacinam foi encontrado que 56,11% das fêmeas tinham títulos HI ≥ 1: 2560. O analise da resposta imune depois de varias doses de vacina nestes rebanhos demonstra que múltiplas vacinações pós-parto podem bloquear a resposta imunológica. Destes resultados surge que PPV está circulando nos rebanhos suínos da Bahia e que segundo os títulos HI detectados, as reprodutoras destes rebanhos vacinados ou não, estariam protegidas contra a infecção viral.

Revista de Ciências Médicas e Biológicas, 2009

Brazilian Journal of Veterinary Research and Animal Science, 2003

Revista da Associação Médica Brasileira

OBJECTIVE: Genome sequencing has been proved to be an excellent tool to monitor the molecular epi... more OBJECTIVE: Genome sequencing has been proved to be an excellent tool to monitor the molecular epidemiology of the disease caused by severe acute respiratory syndrome coronavirus 2, i.e., coronavirus disease 2019. Some reports of infected, vaccinated individuals have aroused great interest because they are primarily being infected with circulating variants of concern. To investigate the cases of infected, vaccinated individuals in Salvador, Bahia, Brazil, we performed genomic monitoring to estimate the magnitude of the different variants of concern in these cases. METHODS: Nasopharyngeal swabs from infected (symptomatic and asymptomatic), vaccinated or unvaccinated individuals (n=29), and quantitative reverse transcription polymerase chain reaction cycle threshold value (Ct values) of ≤30 were subjected to viral sequencing using nanopore technology. RESULTS: Our analysis revealed that the Omicron variant was found in 99% of cases and the Delta variant was found in only one case. Infected, fully vaccinated patients have a favorable clinical prognosis; however, within the community, they become viral carriers with the aggravating factor of viral dissemination of variants of concern not neutralized by the currently available vaccines. CONCLUSION: It is important to acknowledge the limitations of these vaccines and to develop new vaccines to emergent variants of concern, as is the case of influenza vaccine; going through new doses of the same coronavirus vaccines is "more of the same. "

PLOS Neglected Tropical Diseases, 2021

Background Chikungunya is an arbovirus, transmitted by Aedes mosquitoes, which emerged in the Ame... more Background Chikungunya is an arbovirus, transmitted by Aedes mosquitoes, which emerged in the Americas in 2013 and spread rapidly to almost every country on this continent. In Brazil, where the first cases were detected in 2014, it currently has reached all regions of this country and more than 900,000 cases were reported. The clinical spectrum of chikungunya ranges from an acute self-limiting form to disabling chronic forms. The purpose of this study was to estimate the seroprevalence of chikungunya infection in a large Brazilian city and investigate the association between viral circulation and living condition. Methodology/principal findings We conducted a population-based ecological study in selected Sentinel Areas (SA) through household interviews and a serologic survey in 2016/2017. The sample was of 1,981 individuals randomly selected. The CHIKV seroprevalence was 22.1% (17.1 IgG, 2.3 IgM, and 1.4 IgG and IgM) and varied between SA from 2.0% to 70.5%. The seroprevalence was s...

The contagious ecthyma is a disease caused by Orf virus, which induces acute skin pustules lesion... more The contagious ecthyma is a disease caused by Orf virus, which induces acute skin pustules lesions in sheep and goats. In Brazil the frequency of Orf virus infection seems to be higher than that officially reported. This study is the first report on the Orf virus identification and laboratory confirmation in herds of goats in Bahia. The State Agency of Agriculture Defense of Bahia reported the occurrence of an epithelial disease in goats of Caninde and British Alpine breeds in the Barreira and Pedra alta localities. The lesions were characterized by the occurrence of scabs around the labial fold or being restricted to the udders. The samples from lesions scab were collected from goats labial fold and teats . DNA samples were extracted from these materials, and analyzed by polymerase chain reaction (PCR), and the genes ORFV 011 (B2L, 1022 base pairs [bp]) and ORFV 059 (F1L, 1062 bp) were amplified. All of the samples showed positive PCR reaction, confirming the Orf virus infection in...