Guilherme Oliveira - Academia.edu (original) (raw)

Papers by Guilherme Oliveira

Abstract Schistosoma mansoni is responsible for the neglected tropical disease schistosomiasis th... more Abstract Schistosoma mansoni is responsible for the neglected tropical disease schistosomiasis that affects 210 million people in 76 countries. Here we present analysis of the 363 megabase nuclear genome of the blood fluke. It encodes at least 11,809 genes, with an unusual intron size distribution, and new families of micro-exon genes that undergo frequent alternative splicing.

The identification and description of signal transduction molecules and mechanisms are essential ... more The identification and description of signal transduction molecules and mechanisms are essential to elucidate Schistosoma mansoni host-parasite interactions and parasite biology. This mini review focuses on recent advancements in the study of signalling molecules and transduction mechanisms in S. mansoni, drawing special attention to the recently identified and characterised protein tyrosine kinases of S. mansoni.

Biomphalaria tenagophila is very important for schistosomiasis transmission in Brazil. However it... more Biomphalaria tenagophila is very important for schistosomiasis transmission in Brazil. However its mechanisms of interaction with Schistosoma mansoni are still scantly studied. Since this snail displays strains highly susceptible or completely resistant to the parasite infection, the knowledge of that would be a useful tool to understand the mechanism of snail resistance. Particularly, the Taim strain consistently shows absolute resistance against the trematode, and this resistance is a dominant character.

Page 1. Chapter 1 Multiple Regression for the Schistosomiasis Positivity Index Estimates in the M... more Page 1. Chapter 1 Multiple Regression for the Schistosomiasis Positivity Index Estimates in the Minas Gerais State - Brazil at Small Communities and Cities Levels Ricardo JPS Guimarães, Corina C. Freitas, Luciano V. Dutra, Guilherme Oliveira and Omar S. Carvalho Additional information is available at the end of the chapter http://dx.doi.org/10.5772/53500 1.

Background Schistosoma mansoni is one of the causative agents of schistosomiasis, a neglected tro... more Background Schistosoma mansoni is one of the causative agents of schistosomiasis, a neglected tropical disease that affects about 237 million people worldwide. Despite recent efforts, we still lack a general understanding of the relevant host-parasite interactions, and the possible treatments are limited by the emergence of resistant strains and the absence of a vaccine. The S. mansoni genome was completely sequenced and still under continuous annotation.

Schistosomiasis is a prevalent and chronic helmintic disease in tropical regions. Treatment and c... more Schistosomiasis is a prevalent and chronic helmintic disease in tropical regions. Treatment and control relies on chemotherapy with just one drug, praziquantel and this reliance is of concern should clinically relevant drug resistance emerge and spread.

SUMMARY Schistosome parasites exhibit separate sexes and with the evolution of sex they have deve... more SUMMARY Schistosome parasites exhibit separate sexes and with the evolution of sex they have developed an intricate relationship between the male and female worms such that signals between the male and female that are initiated at the time of mating, regulate female reproductive development and subsequent egg production.

To study characteristics of neurological disorders in HIV/AIDS patients and their relationship to... more To study characteristics of neurological disorders in HIV/AIDS patients and their relationship to highly active antiretroviral treatment, a cross-sectional study was conducted in an infectious disease public hospital in Belo Horizonte, Brazil, between February 1999 and March 2000. Of the 417 patients enrolled, neurological disease was observed in 194 (46.5%) and a new AIDS-defining neurological event developed in 23.7% of individuals.

Background MicroRNAs (miRNAs) constitute a class of single-stranded RNAs which play a crucial rol... more Background MicroRNAs (miRNAs) constitute a class of single-stranded RNAs which play a crucial role in regulating development and controlling gene expression by targeting mRNAs and triggering either translation repression or messenger RNA (mRNA) degradation. miRNAs are widespread in eukaryotes and to date over 14,000 miRNAs have been identified by computational and experimental approaches. Several miRNAs are highly conserved across species.

Abstract The saliva of the sand fly Lutzomyia longipalpis, a major vector of Leishmania, exhibits... more Abstract The saliva of the sand fly Lutzomyia longipalpis, a major vector of Leishmania, exhibits pharmacological and immunomodulatory activities that may facilitate entry and establishment of parasites into the vertebrate host. Salivary gland components of the sand fly are, therefore, potential candidates in the development of a vaccine against human leishmaniasis.

Sequence variation among different hepatitis C virus (HCV) isolates has adaptive significance and... more Sequence variation among different hepatitis C virus (HCV) isolates has adaptive significance and reflects the modes and intensities of selection mechanisms operating on the virus. In this work, we sought to investigate using classical population genetics parameters, the genetic variability of HCV genotype 1 using the 5'UTR and NS5A regions from treatment non-responding and responding groups of patients. Both regions showed low genetic varia-bility and the 5'UTR showed neutral deviation.

The parasite Schistosoma is known to exhibit variations among species, strains and genera, such a... more The parasite Schistosoma is known to exhibit variations among species, strains and genera, such as, the levels of infectivity, pathogenicity and immunogenicity. These factors may differ among parasite populations according to the local epidemiological conditions. Diversity observed in Schistosoma mansoni from different geographical regions or within individuals of the same region can be determined by differences in the genotype of each parasite strain.

Identification of major schistosome antigens recognized by patients from endemic areas have long ... more Identification of major schistosome antigens recognized by patients from endemic areas have long been a goal in the studies of the immune response to the schistosome infection. The objectives are several, including identification of major antigens to be used in imunodiagnosis (Mott & Dixon, 1982; Mott, 1984; Tsanget al., 1984; Ruppel et al., 1985; Ruppel et al., 1987) and vaccines (Horowitz et al., 1982; Smit & Clegg, 1985; James, 1984; James et al., 1985).

Background Corynebacterium pseudotuberculosis, a Gram-positive, facultative intracellular pathoge... more Background Corynebacterium pseudotuberculosis, a Gram-positive, facultative intracellular pathogen, is the etiologic agent of the disease known as caseous lymphadenitis (CL). CL mainly affects small ruminants, such as goats and sheep; it also causes infections in humans, though rarely. This species is distributed worldwide, but it has the most serious economic impact in Oceania, Africa and South America. Although C.

In this paper we review the impact that the availability of the Schistosoma mansoni genome sequen... more In this paper we review the impact that the availability of the Schistosoma mansoni genome sequence and annotation has had on schistosomiasis research. Easy access to the genomic information is important and several types of data are currently being integrated, such as proteomics, microarray and polymorphic loci. Access to the genome annotation and powerful means of extracting information are major resources to the research community.

Equipment–The funds requested for equipment are for acquisition of microcentrifuge, pipettors, PC... more Equipment–The funds requested for equipment are for acquisition of microcentrifuge, pipettors, PCR machine, electrophoresis apparatus, photo documentation equipment, fridge and freezer for the laboratory in Nigeria. Equipment purchase will be spread over the two years of the project. In Nigeria the available equipment is currently shared with the malaria research team.

CA88 is the first long nuclear repetitive DNA sequence identified in the blood fluke, Schistosoma... more CA88 is the first long nuclear repetitive DNA sequence identified in the blood fluke, Schistosoma mansoni. The assembled S. mansoni sequence, which contains the CA88 repeat, has 8,887 nucleotides and at least three repeat units of approximately 360 bp. In addition, CA88 also possesses an internal CA microsatellite, identified as SmBr18. Both PCR and BLAST analysis have been used to analyse and confirm the CA88 sequence in other S. mansoni sequences in the public database.

RESUMO As hemofilias são doenças hemorrágicas resultantes da deficiência de fator VIII (hemofilia... more RESUMO As hemofilias são doenças hemorrágicas resultantes da deficiência de fator VIII (hemofilia A) ou de fator IX (hemofilia B) da coagulação, decorrentes de mutações nos genes que codificam os fatores VIII ou IX, respectivamente. A hemofilia A é mais frequente que a hemofilia B e acomete aproximadamente 1: 10.000 nascimentos masculinos.

Protein Tyrosine Kinases (PTKs) are important molecules in intra-and inter-cellular communication... more Protein Tyrosine Kinases (PTKs) are important molecules in intra-and inter-cellular communication, playing a major role in signal transduction processes. We have previously identified and characterized the molecular structure of a new PTK in Schistosoma mansoni, SmFes. SmFes exhibits the characteristic features of Fes/Fps protein tyrosine kinase subfamily of which it is the first member described in helminths.

Background Singular value decomposition (SVD) is a powerful technique for information retrieval; ... more Background Singular value decomposition (SVD) is a powerful technique for information retrieval; it helps uncover relationships between elements that are not prima facie related. SVD was initially developed to reduce the time needed for information retrieval and analysis of very large data sets in the complex internet environment. Since information retrieval from large-scale genome and proteome data sets has a similar level of complexity, SVD-based methods could also facilitate data analysis in this research area.

Abstract Schistosoma mansoni is responsible for the neglected tropical disease schistosomiasis th... more Abstract Schistosoma mansoni is responsible for the neglected tropical disease schistosomiasis that affects 210 million people in 76 countries. Here we present analysis of the 363 megabase nuclear genome of the blood fluke. It encodes at least 11,809 genes, with an unusual intron size distribution, and new families of micro-exon genes that undergo frequent alternative splicing.

The identification and description of signal transduction molecules and mechanisms are essential ... more The identification and description of signal transduction molecules and mechanisms are essential to elucidate Schistosoma mansoni host-parasite interactions and parasite biology. This mini review focuses on recent advancements in the study of signalling molecules and transduction mechanisms in S. mansoni, drawing special attention to the recently identified and characterised protein tyrosine kinases of S. mansoni.

Biomphalaria tenagophila is very important for schistosomiasis transmission in Brazil. However it... more Biomphalaria tenagophila is very important for schistosomiasis transmission in Brazil. However its mechanisms of interaction with Schistosoma mansoni are still scantly studied. Since this snail displays strains highly susceptible or completely resistant to the parasite infection, the knowledge of that would be a useful tool to understand the mechanism of snail resistance. Particularly, the Taim strain consistently shows absolute resistance against the trematode, and this resistance is a dominant character.

Page 1. Chapter 1 Multiple Regression for the Schistosomiasis Positivity Index Estimates in the M... more Page 1. Chapter 1 Multiple Regression for the Schistosomiasis Positivity Index Estimates in the Minas Gerais State - Brazil at Small Communities and Cities Levels Ricardo JPS Guimarães, Corina C. Freitas, Luciano V. Dutra, Guilherme Oliveira and Omar S. Carvalho Additional information is available at the end of the chapter http://dx.doi.org/10.5772/53500 1.

Background Schistosoma mansoni is one of the causative agents of schistosomiasis, a neglected tro... more Background Schistosoma mansoni is one of the causative agents of schistosomiasis, a neglected tropical disease that affects about 237 million people worldwide. Despite recent efforts, we still lack a general understanding of the relevant host-parasite interactions, and the possible treatments are limited by the emergence of resistant strains and the absence of a vaccine. The S. mansoni genome was completely sequenced and still under continuous annotation.

Schistosomiasis is a prevalent and chronic helmintic disease in tropical regions. Treatment and c... more Schistosomiasis is a prevalent and chronic helmintic disease in tropical regions. Treatment and control relies on chemotherapy with just one drug, praziquantel and this reliance is of concern should clinically relevant drug resistance emerge and spread.

SUMMARY Schistosome parasites exhibit separate sexes and with the evolution of sex they have deve... more SUMMARY Schistosome parasites exhibit separate sexes and with the evolution of sex they have developed an intricate relationship between the male and female worms such that signals between the male and female that are initiated at the time of mating, regulate female reproductive development and subsequent egg production.

To study characteristics of neurological disorders in HIV/AIDS patients and their relationship to... more To study characteristics of neurological disorders in HIV/AIDS patients and their relationship to highly active antiretroviral treatment, a cross-sectional study was conducted in an infectious disease public hospital in Belo Horizonte, Brazil, between February 1999 and March 2000. Of the 417 patients enrolled, neurological disease was observed in 194 (46.5%) and a new AIDS-defining neurological event developed in 23.7% of individuals.

Background MicroRNAs (miRNAs) constitute a class of single-stranded RNAs which play a crucial rol... more Background MicroRNAs (miRNAs) constitute a class of single-stranded RNAs which play a crucial role in regulating development and controlling gene expression by targeting mRNAs and triggering either translation repression or messenger RNA (mRNA) degradation. miRNAs are widespread in eukaryotes and to date over 14,000 miRNAs have been identified by computational and experimental approaches. Several miRNAs are highly conserved across species.

Abstract The saliva of the sand fly Lutzomyia longipalpis, a major vector of Leishmania, exhibits... more Abstract The saliva of the sand fly Lutzomyia longipalpis, a major vector of Leishmania, exhibits pharmacological and immunomodulatory activities that may facilitate entry and establishment of parasites into the vertebrate host. Salivary gland components of the sand fly are, therefore, potential candidates in the development of a vaccine against human leishmaniasis.

Sequence variation among different hepatitis C virus (HCV) isolates has adaptive significance and... more Sequence variation among different hepatitis C virus (HCV) isolates has adaptive significance and reflects the modes and intensities of selection mechanisms operating on the virus. In this work, we sought to investigate using classical population genetics parameters, the genetic variability of HCV genotype 1 using the 5'UTR and NS5A regions from treatment non-responding and responding groups of patients. Both regions showed low genetic varia-bility and the 5'UTR showed neutral deviation.

The parasite Schistosoma is known to exhibit variations among species, strains and genera, such a... more The parasite Schistosoma is known to exhibit variations among species, strains and genera, such as, the levels of infectivity, pathogenicity and immunogenicity. These factors may differ among parasite populations according to the local epidemiological conditions. Diversity observed in Schistosoma mansoni from different geographical regions or within individuals of the same region can be determined by differences in the genotype of each parasite strain.

Identification of major schistosome antigens recognized by patients from endemic areas have long ... more Identification of major schistosome antigens recognized by patients from endemic areas have long been a goal in the studies of the immune response to the schistosome infection. The objectives are several, including identification of major antigens to be used in imunodiagnosis (Mott & Dixon, 1982; Mott, 1984; Tsanget al., 1984; Ruppel et al., 1985; Ruppel et al., 1987) and vaccines (Horowitz et al., 1982; Smit & Clegg, 1985; James, 1984; James et al., 1985).

Background Corynebacterium pseudotuberculosis, a Gram-positive, facultative intracellular pathoge... more Background Corynebacterium pseudotuberculosis, a Gram-positive, facultative intracellular pathogen, is the etiologic agent of the disease known as caseous lymphadenitis (CL). CL mainly affects small ruminants, such as goats and sheep; it also causes infections in humans, though rarely. This species is distributed worldwide, but it has the most serious economic impact in Oceania, Africa and South America. Although C.

In this paper we review the impact that the availability of the Schistosoma mansoni genome sequen... more In this paper we review the impact that the availability of the Schistosoma mansoni genome sequence and annotation has had on schistosomiasis research. Easy access to the genomic information is important and several types of data are currently being integrated, such as proteomics, microarray and polymorphic loci. Access to the genome annotation and powerful means of extracting information are major resources to the research community.

Equipment–The funds requested for equipment are for acquisition of microcentrifuge, pipettors, PC... more Equipment–The funds requested for equipment are for acquisition of microcentrifuge, pipettors, PCR machine, electrophoresis apparatus, photo documentation equipment, fridge and freezer for the laboratory in Nigeria. Equipment purchase will be spread over the two years of the project. In Nigeria the available equipment is currently shared with the malaria research team.

CA88 is the first long nuclear repetitive DNA sequence identified in the blood fluke, Schistosoma... more CA88 is the first long nuclear repetitive DNA sequence identified in the blood fluke, Schistosoma mansoni. The assembled S. mansoni sequence, which contains the CA88 repeat, has 8,887 nucleotides and at least three repeat units of approximately 360 bp. In addition, CA88 also possesses an internal CA microsatellite, identified as SmBr18. Both PCR and BLAST analysis have been used to analyse and confirm the CA88 sequence in other S. mansoni sequences in the public database.

RESUMO As hemofilias são doenças hemorrágicas resultantes da deficiência de fator VIII (hemofilia... more RESUMO As hemofilias são doenças hemorrágicas resultantes da deficiência de fator VIII (hemofilia A) ou de fator IX (hemofilia B) da coagulação, decorrentes de mutações nos genes que codificam os fatores VIII ou IX, respectivamente. A hemofilia A é mais frequente que a hemofilia B e acomete aproximadamente 1: 10.000 nascimentos masculinos.

Protein Tyrosine Kinases (PTKs) are important molecules in intra-and inter-cellular communication... more Protein Tyrosine Kinases (PTKs) are important molecules in intra-and inter-cellular communication, playing a major role in signal transduction processes. We have previously identified and characterized the molecular structure of a new PTK in Schistosoma mansoni, SmFes. SmFes exhibits the characteristic features of Fes/Fps protein tyrosine kinase subfamily of which it is the first member described in helminths.

Background Singular value decomposition (SVD) is a powerful technique for information retrieval; ... more Background Singular value decomposition (SVD) is a powerful technique for information retrieval; it helps uncover relationships between elements that are not prima facie related. SVD was initially developed to reduce the time needed for information retrieval and analysis of very large data sets in the complex internet environment. Since information retrieval from large-scale genome and proteome data sets has a similar level of complexity, SVD-based methods could also facilitate data analysis in this research area.