Guy COSTA - Academia.edu (original) (raw)
Papers by Guy COSTA
Mycorrhiza Manual, 1998
Identification of individual genets (clones) is essential for a better understanding of the diver... more Identification of individual genets (clones) is essential for a better understanding of the diversity, structure, and dynamic of populations of ectomycorrhizal fungi. Genets have been identified on the basis of their somatic incompatibility (Dahlberg 1991). Unfortunately, this approach is time-consuming and can be inconclusive (Jacobson et al. 1993). Random amplification of polymorphic DNA (RAPD) (Welsh and McClelland 1990; Williams et al. 1990) has therefore been used for the analysis of populations of Suillus granulatus (Jacobson et al. 1993) and Laccaria bicolor (Buschena et al. 1992; De la Bastide et al. 1993). However, this technique has been reported to be very sensitive to experimental variables (Devos and Gale 1992), and RAPD assay conditions described for one species may not be suitable with another (Klein-Lankhorst et al. 1991). These difficulties are mainly related to the low stringency necessary for successful RAPD amplification (Bachman 1993).
BMC Genomic Data
Objectives Molecular cues linked to heartwood formation open new (complementary) perspectives to ... more Objectives Molecular cues linked to heartwood formation open new (complementary) perspectives to genetic breeding programs of Douglas-fir, a tree species largely cultivated in Europe for the natural durability and civil engineering properties of its wood. Data description RNAs from a single genotype of Douglas-fir, extracted from three distinct wood zones (outer sapwood, inner sapwood and transition zone) at four vegetative seasons to generate an extensive RNA-seq dataset used to apprehend the in-wood dynamic and seasonality of heartwood formation in this hardwood model species. Previously published data collected on somatic embryos of the same genotype could be merged with the present dataset to upgrade grade the Douglas-fir reference transcriptome.
HAL (Le Centre pour la Communication Scientifique Directe), Jul 9, 2021
HAL (Le Centre pour la Communication Scientifique Directe), Sep 19, 2016
HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific re... more HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.
Biointerface Research in Applied Chemistry, 2020
This work aims to extrapolate the energy conversion of Raw Loquat Kernels (RLK) by pyrolysis unde... more This work aims to extrapolate the energy conversion of Raw Loquat Kernels (RLK) by pyrolysis under the inert atmosphere. For this purpose, the main physicochemical composition of the RLK was first investigated. The proximate analysis shows that RLK has a relatively low moisture content, an absence of heavy metals, and the presence of an important amount of organic matter. In order to evaluate polysaccharides composition, RLK was dissolved in 1-ethyl-3-methylimidazolium bromide [Emim]Br ionic liquid; the resulting solutions are then immuno-labeled with monoclonal Antibodies (mAbs) against plant cell wall polysaccharides epitopes. Polysaccharides have been then analyzed using the ELISA technique with a set of 14 mAbs against hemicellulose and pectin. The LM19 immuno-labeling was more intense after 3 days of treatment at 80 °C, followed by 2 hours at 100 °C, indicating the important amount of un-esterified Homogalacturonans among RLK polysaccharides. X-ray diffraction pattern indicates...
International audienceDouglas-fir (Pseudotsuga menziesii (Mirb) Franco) is a native conifer from ... more International audienceDouglas-fir (Pseudotsuga menziesii (Mirb) Franco) is a native conifer from the Pacific North-West of the US and Canada, and is one of the most important timber species used in the world with both high productivity in a range of climatic conditions and highly valuable wood properties (quality) as well as strong tolerance to diseases and insects. In Europe, Douglas-fir is a major species for reforestation with increasing demand for its wood. Therefore, adaptation of new varieties to climate change and associated stresses is one challenging question for ongoing breeding programs. Efficient selection and vegetative propagation of improved varieties appeared to be key issues to maintain productivity in plantation forestry (Lelu-Walter et al. 2013). However, as in many other conifers, early maturation is preventing clonal forestry through conventional multiplication methods in Douglas-fir (Bastien et al. 2013). Somatic embryogenesis from immature seeds, coupled with ...
The purpose of the present study was to observe the interference genotypes-energy release rate. T... more The purpose of the present study was to observe the interference genotypes-energy release rate. The tests were performed using the wedge splitting samples manufactured from the Douglas genotypes. Four Douglas genotypes were selected in order to observe the influence of genetic background on the mechanical behavior. In order to evaluate the fracture parameters the experimental measurements were realized by using the optical methods such as mark tracking method and digital image correlation. Using the optical measurements the fracture parameters were calculated by using the energetic approaches coupled with the numerical simulations. The moisture content influence was also studied. Then, the experimental tests were performed for different moisture contents.
The Science of the total environment, Jan 8, 2015
In the present study, the residual toxicity and impact of aged nZVI after a leaching experiment o... more In the present study, the residual toxicity and impact of aged nZVI after a leaching experiment on heavy metal (Pb, Zn) polluted soils was evaluated. No negative effects on physico-chemical soil properties were observed after aged nZVI exposure. The application of nZVI to soil produced a significant increase in Fe availability. The impact on soil biodiversity was assessed by fluorescence in situ hybridization (FISH). A significant effect of nZVI application on microbial structure has been recorded in the Pb-polluted soil nZVI-treated. Soil bacteria molecular response, evaluated by RT-qPCR using exposure biomarkers (pykA, katB) showed a decrease in the cellular activity (pykA) due to enhanced intracellular oxidative stress (katB). Moreover, ecotoxicological standardised test on Caenorhabditis elegans (C. elegans) showed a decrease in the growth endpoint in the Pb-polluted soil, and particularly in the nZVI-treated. A different pattern has been observed in Zn-polluted soils: no change...
Science of The Total Environment, 2014
In vitro analyses were conducted to assess the impact of Al2O3 and Ag nanoparticles on two common... more In vitro analyses were conducted to assess the impact of Al2O3 and Ag nanoparticles on two common soil bacteria, Bacillus cereus and Pseudomonas stutzeri. Al2O3 nanoparticles did not show significant toxicity at any dose or time assayed, whereas exposure to 5 mg L(-1) Ag nanoparticles for 48 h caused bactericidal effects. Moreover, alterations at the morphological level were observed by transmission electron microscopy (TEM); Ag but not Al2O3 nanoparticles evoked the entrance of B. cereus cells in an early sporulation stage and both nanoparticles penetrated P. stutzeri cells. At the molecular level, a dramatic increase (8.2-fold) in katB gene expression was found in P. stutzeri following Al2O3 nanoparticles exposure, indicative of an oxidative stress-defence system enhancement in this bacterium. In the microcosm experiment, using two different natural soils, Al2O3 or Ag nanoparticles did not affect the Caenorhabditis elegans toxicity endpoints growth, survival, or reproduction. However, differences in microbial phylogenetic compositions were detected by fluorescence in situ hybridization (FISH). The use of katB- and pykA-based sequences showed that the microbial transcriptional response to nanoparticle exposure decreased, suggesting a decrease in cellular activity. These changes were attributable to both the nanoparticles treatment and soil characteristics, highlighting the importance of considering the soil matrix on a case by case basis.
Chemosphere, 2014
The effects of nZVI on autochthonous organisms of two standard soils were assessed. Soil ecotoxic... more The effects of nZVI on autochthonous organisms of two standard soils were assessed. Soil ecotoxicity tests ruled out a toxic impact of nZVI on Caenorhabditis elegans. nZVI treatment evoked significant changes in the expression of soil microorganisms biomarkers. A molecular approach could anticipate adverse effects on environmental biota.
Chemosphere, 2013
h i g h l i g h t s Effects of NZVI on B. cereus were assessed at a phenotypic and molecular leve... more h i g h l i g h t s Effects of NZVI on B. cereus were assessed at a phenotypic and molecular level. The entrance of the cells in an early sporulation stage was observed by TEM. Proteins involved in oxidative stress-response and TCA cycle were overexpressed. Proteins involved in motility and wall biosynthesis were repressed.
Dans le cadre de cette étude, nous présentons une approche originale de la durabilité en proposan... more Dans le cadre de cette étude, nous présentons une approche originale de la durabilité en proposant une transversalité entre patrimoine génétique de différentes familles de Douglas et durabilité en structure en employant, comme 'marqueur', le comportement hygroscopique du matériau. Cette transversalité entre biologie et mécanique du bois a pour but d'accroitre ce degré de durabilité afin de limiter le recours systématique à ces traitements de préservation souvent agressifs. Ce travail se présente sous forme d'une étude statistique avec ambition de mettre en exergue des génotypes en lien avec un faible coefficient de diffusion et des isothermes de sorption à humidité d'équilibre basse.
Table S3. Total protein content (mean ± SD, n = 4) in proliferating 2ry and 3ry embryogenic lines... more Table S3. Total protein content (mean ± SD, n = 4) in proliferating 2ry and 3ry embryogenic lines of Douglas-fir. (DOCX 45 kb)
Figure S3. Histology of non-embryogenic cells (NECs) clusters from 1ry and/or 2ry lines of genoty... more Figure S3. Histology of non-embryogenic cells (NECs) clusters from 1ry and/or 2ry lines of genotypes TD17 and SD4. A / Histology of TD17 groups of loosely arranged NECs (arrow) in the vicinity of small somatic embryos (arrowhead); B / histology of TD17 compact NEC cluster accumulating phenolics (blue and brown cells, arrowheads) and starch (arrow); C / Lugol staining of TD17 NEC cluster showing starch (arrows) and phenolic compounds (small dark granules marked with arrowhead); D / TD17–1 NEC cluster with dividing cells (arrowheads); E /SD4–2 NEC cluster (arrow) within polyembryogenic center besides well-arranged embryonal heads (EH), note phenolic content (in dark blue-grey) of cells separating NEC from suspensor cells (in light blue); F / SD4–6 meristemoid-like NEC cluster; note phenolic content of cells between two meristemoid-like structures (in blue-grey). Scale bar: A, E = 200 μm; B, F = 100 μm; C, D = 50 μm. (DOCX 417 kb)
Mycorrhiza Manual, 1998
Identification of individual genets (clones) is essential for a better understanding of the diver... more Identification of individual genets (clones) is essential for a better understanding of the diversity, structure, and dynamic of populations of ectomycorrhizal fungi. Genets have been identified on the basis of their somatic incompatibility (Dahlberg 1991). Unfortunately, this approach is time-consuming and can be inconclusive (Jacobson et al. 1993). Random amplification of polymorphic DNA (RAPD) (Welsh and McClelland 1990; Williams et al. 1990) has therefore been used for the analysis of populations of Suillus granulatus (Jacobson et al. 1993) and Laccaria bicolor (Buschena et al. 1992; De la Bastide et al. 1993). However, this technique has been reported to be very sensitive to experimental variables (Devos and Gale 1992), and RAPD assay conditions described for one species may not be suitable with another (Klein-Lankhorst et al. 1991). These difficulties are mainly related to the low stringency necessary for successful RAPD amplification (Bachman 1993).
BMC Genomic Data
Objectives Molecular cues linked to heartwood formation open new (complementary) perspectives to ... more Objectives Molecular cues linked to heartwood formation open new (complementary) perspectives to genetic breeding programs of Douglas-fir, a tree species largely cultivated in Europe for the natural durability and civil engineering properties of its wood. Data description RNAs from a single genotype of Douglas-fir, extracted from three distinct wood zones (outer sapwood, inner sapwood and transition zone) at four vegetative seasons to generate an extensive RNA-seq dataset used to apprehend the in-wood dynamic and seasonality of heartwood formation in this hardwood model species. Previously published data collected on somatic embryos of the same genotype could be merged with the present dataset to upgrade grade the Douglas-fir reference transcriptome.
HAL (Le Centre pour la Communication Scientifique Directe), Jul 9, 2021
HAL (Le Centre pour la Communication Scientifique Directe), Sep 19, 2016
HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific re... more HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.
Biointerface Research in Applied Chemistry, 2020
This work aims to extrapolate the energy conversion of Raw Loquat Kernels (RLK) by pyrolysis unde... more This work aims to extrapolate the energy conversion of Raw Loquat Kernels (RLK) by pyrolysis under the inert atmosphere. For this purpose, the main physicochemical composition of the RLK was first investigated. The proximate analysis shows that RLK has a relatively low moisture content, an absence of heavy metals, and the presence of an important amount of organic matter. In order to evaluate polysaccharides composition, RLK was dissolved in 1-ethyl-3-methylimidazolium bromide [Emim]Br ionic liquid; the resulting solutions are then immuno-labeled with monoclonal Antibodies (mAbs) against plant cell wall polysaccharides epitopes. Polysaccharides have been then analyzed using the ELISA technique with a set of 14 mAbs against hemicellulose and pectin. The LM19 immuno-labeling was more intense after 3 days of treatment at 80 °C, followed by 2 hours at 100 °C, indicating the important amount of un-esterified Homogalacturonans among RLK polysaccharides. X-ray diffraction pattern indicates...
International audienceDouglas-fir (Pseudotsuga menziesii (Mirb) Franco) is a native conifer from ... more International audienceDouglas-fir (Pseudotsuga menziesii (Mirb) Franco) is a native conifer from the Pacific North-West of the US and Canada, and is one of the most important timber species used in the world with both high productivity in a range of climatic conditions and highly valuable wood properties (quality) as well as strong tolerance to diseases and insects. In Europe, Douglas-fir is a major species for reforestation with increasing demand for its wood. Therefore, adaptation of new varieties to climate change and associated stresses is one challenging question for ongoing breeding programs. Efficient selection and vegetative propagation of improved varieties appeared to be key issues to maintain productivity in plantation forestry (Lelu-Walter et al. 2013). However, as in many other conifers, early maturation is preventing clonal forestry through conventional multiplication methods in Douglas-fir (Bastien et al. 2013). Somatic embryogenesis from immature seeds, coupled with ...
The purpose of the present study was to observe the interference genotypes-energy release rate. T... more The purpose of the present study was to observe the interference genotypes-energy release rate. The tests were performed using the wedge splitting samples manufactured from the Douglas genotypes. Four Douglas genotypes were selected in order to observe the influence of genetic background on the mechanical behavior. In order to evaluate the fracture parameters the experimental measurements were realized by using the optical methods such as mark tracking method and digital image correlation. Using the optical measurements the fracture parameters were calculated by using the energetic approaches coupled with the numerical simulations. The moisture content influence was also studied. Then, the experimental tests were performed for different moisture contents.
The Science of the total environment, Jan 8, 2015
In the present study, the residual toxicity and impact of aged nZVI after a leaching experiment o... more In the present study, the residual toxicity and impact of aged nZVI after a leaching experiment on heavy metal (Pb, Zn) polluted soils was evaluated. No negative effects on physico-chemical soil properties were observed after aged nZVI exposure. The application of nZVI to soil produced a significant increase in Fe availability. The impact on soil biodiversity was assessed by fluorescence in situ hybridization (FISH). A significant effect of nZVI application on microbial structure has been recorded in the Pb-polluted soil nZVI-treated. Soil bacteria molecular response, evaluated by RT-qPCR using exposure biomarkers (pykA, katB) showed a decrease in the cellular activity (pykA) due to enhanced intracellular oxidative stress (katB). Moreover, ecotoxicological standardised test on Caenorhabditis elegans (C. elegans) showed a decrease in the growth endpoint in the Pb-polluted soil, and particularly in the nZVI-treated. A different pattern has been observed in Zn-polluted soils: no change...
Science of The Total Environment, 2014
In vitro analyses were conducted to assess the impact of Al2O3 and Ag nanoparticles on two common... more In vitro analyses were conducted to assess the impact of Al2O3 and Ag nanoparticles on two common soil bacteria, Bacillus cereus and Pseudomonas stutzeri. Al2O3 nanoparticles did not show significant toxicity at any dose or time assayed, whereas exposure to 5 mg L(-1) Ag nanoparticles for 48 h caused bactericidal effects. Moreover, alterations at the morphological level were observed by transmission electron microscopy (TEM); Ag but not Al2O3 nanoparticles evoked the entrance of B. cereus cells in an early sporulation stage and both nanoparticles penetrated P. stutzeri cells. At the molecular level, a dramatic increase (8.2-fold) in katB gene expression was found in P. stutzeri following Al2O3 nanoparticles exposure, indicative of an oxidative stress-defence system enhancement in this bacterium. In the microcosm experiment, using two different natural soils, Al2O3 or Ag nanoparticles did not affect the Caenorhabditis elegans toxicity endpoints growth, survival, or reproduction. However, differences in microbial phylogenetic compositions were detected by fluorescence in situ hybridization (FISH). The use of katB- and pykA-based sequences showed that the microbial transcriptional response to nanoparticle exposure decreased, suggesting a decrease in cellular activity. These changes were attributable to both the nanoparticles treatment and soil characteristics, highlighting the importance of considering the soil matrix on a case by case basis.
Chemosphere, 2014
The effects of nZVI on autochthonous organisms of two standard soils were assessed. Soil ecotoxic... more The effects of nZVI on autochthonous organisms of two standard soils were assessed. Soil ecotoxicity tests ruled out a toxic impact of nZVI on Caenorhabditis elegans. nZVI treatment evoked significant changes in the expression of soil microorganisms biomarkers. A molecular approach could anticipate adverse effects on environmental biota.
Chemosphere, 2013
h i g h l i g h t s Effects of NZVI on B. cereus were assessed at a phenotypic and molecular leve... more h i g h l i g h t s Effects of NZVI on B. cereus were assessed at a phenotypic and molecular level. The entrance of the cells in an early sporulation stage was observed by TEM. Proteins involved in oxidative stress-response and TCA cycle were overexpressed. Proteins involved in motility and wall biosynthesis were repressed.
Dans le cadre de cette étude, nous présentons une approche originale de la durabilité en proposan... more Dans le cadre de cette étude, nous présentons une approche originale de la durabilité en proposant une transversalité entre patrimoine génétique de différentes familles de Douglas et durabilité en structure en employant, comme 'marqueur', le comportement hygroscopique du matériau. Cette transversalité entre biologie et mécanique du bois a pour but d'accroitre ce degré de durabilité afin de limiter le recours systématique à ces traitements de préservation souvent agressifs. Ce travail se présente sous forme d'une étude statistique avec ambition de mettre en exergue des génotypes en lien avec un faible coefficient de diffusion et des isothermes de sorption à humidité d'équilibre basse.
Table S3. Total protein content (mean ± SD, n = 4) in proliferating 2ry and 3ry embryogenic lines... more Table S3. Total protein content (mean ± SD, n = 4) in proliferating 2ry and 3ry embryogenic lines of Douglas-fir. (DOCX 45 kb)
Figure S3. Histology of non-embryogenic cells (NECs) clusters from 1ry and/or 2ry lines of genoty... more Figure S3. Histology of non-embryogenic cells (NECs) clusters from 1ry and/or 2ry lines of genotypes TD17 and SD4. A / Histology of TD17 groups of loosely arranged NECs (arrow) in the vicinity of small somatic embryos (arrowhead); B / histology of TD17 compact NEC cluster accumulating phenolics (blue and brown cells, arrowheads) and starch (arrow); C / Lugol staining of TD17 NEC cluster showing starch (arrows) and phenolic compounds (small dark granules marked with arrowhead); D / TD17–1 NEC cluster with dividing cells (arrowheads); E /SD4–2 NEC cluster (arrow) within polyembryogenic center besides well-arranged embryonal heads (EH), note phenolic content (in dark blue-grey) of cells separating NEC from suspensor cells (in light blue); F / SD4–6 meristemoid-like NEC cluster; note phenolic content of cells between two meristemoid-like structures (in blue-grey). Scale bar: A, E = 200 μm; B, F = 100 μm; C, D = 50 μm. (DOCX 417 kb)