H. Bauwe - Academia.edu (original) (raw)

Papers by H. Bauwe

Research paper thumbnail of Arabidopsis Mutants with Strongly Reduced Levels of the T-Protein Subunit of Glycine Decarboxylase

Photosynthesis. Energy from the Sun, 2008

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Research paper thumbnail of Oxalate Decarboxylase is Involved in Turnover of 2-Phosphoglycolate in Synechocystis sp. Strain PCC 6803

Photosynthesis. Energy from the Sun, 2008

Despite the presence of a carbon concentrating mechanism, cyanobacteria synthesize 2- phosphoglyc... more Despite the presence of a carbon concentrating mechanism, cyanobacteria synthesize 2- phosphoglycolate (2PG) by the oxygenase reaction of Rubisco. Using DNA-microarray analyses, we examined the regulation of genes involved in 2PG metabolism in the cyanobacterium Synechocystis PCC 6803. While genes for the plant-like C2 cycle were barely influenced by low Ci conditions, genes of the bacterial-like glycerate pathway were upregulated.

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Research paper thumbnail of Lipoate-Protein Ligase and Octanoyltransferase Are Essential for Protein Lipoylation in Mitochondria of Arabidopsis

Plant physiology, Jan 28, 2014

Prosthetic lipoyl groups are required for the function of several essential multienzyme complexes... more Prosthetic lipoyl groups are required for the function of several essential multienzyme complexes, such as pyruvate dehydrogenase (PDH), α-ketoglutarate dehydrogenase (KGDH), and the glycine cleavage system (glycine decarboxylase [GDC]). How these proteins are lipoylated has been extensively studied in prokaryotes and yeast (Saccharomyces cerevisiae), but little is known for plants. We earlier reported that mitochondrial fatty acid synthesis by ketoacyl-acyl carrier protein synthase is not vital for protein lipoylation in Arabidopsis (Arabidopsis thaliana) and does not play a significant role in roots. Here, we identify Arabidopsis lipoate-protein ligase (AtLPLA) as an essential mitochondrial enzyme that uses octanoyl-nucleoside monophosphate and possibly other donor substrates for the octanoylation of mitochondrial PDH-E2 and GDC H-protein; it shows no reactivity with bacterial and possibly plant KGDH-E2. The octanoate-activating enzyme is unknown, but we assume that it uses octano...

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Research paper thumbnail of C2 photosynthesis generates about 3-fold elevated leaf CO2 levels in the C3-C4 intermediate species Flaveria pubescens

Journal of experimental botany, 2014

Formation of a photorespiration-based CO2-concentrating mechanism in C3-C4 intermediate plants is... more Formation of a photorespiration-based CO2-concentrating mechanism in C3-C4 intermediate plants is seen as a prerequisite for the evolution of C4 photosynthesis, but it is not known how efficient this mechanism is. Here, using in vivo Rubisco carboxylation-to-oxygenation ratios as a proxy to assess relative intraplastidial CO2 levels is suggested. Such ratios were determined for the C3-C4 intermediate species Flaveria pubescens compared with the closely related C3 plant F. cronquistii and the C4 plant F. trinervia. To this end, a model was developed to describe the major carbon fluxes and metabolite pools involved in photosynthetic-photorespiratory carbon metabolism and used quantitatively to evaluate the labelling kinetics during short-term (14)CO2 incorporation. Our data suggest that the photorespiratory CO2 pump elevates the intraplastidial CO2 concentration about 3-fold in leaves of the C3-C4 intermediate species F. pubescens relative to the C3 species F. cronquistii.

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Research paper thumbnail of Expression profiling of the bloom-forming cyanobacterium Nodularia CCY9414 under light and oxidative stress conditions

The ISME Journal, 2015

Massive blooms of toxic cyanobacteria frequently occur in the central Baltic Sea during the summe... more Massive blooms of toxic cyanobacteria frequently occur in the central Baltic Sea during the summer. In the surface scum, cyanobacterial cells are exposed to high light (HL) intensity, high oxygen partial pressure and other stresses. To mimic these conditions, cultures of Nodularia spumigena CCY9414, which is a strain isolated from a cyanobacterial summer bloom in the Baltic Sea, were incubated at a HL intensity of 1200 μmol photons m(-2) s(-1) or a combination of HL and increased oxygen partial pressure. Using differential RNA sequencing, we compared the global primary transcriptomes of control and stressed cells. The combination of oxidative and light stresses induced the expression of twofold more genes compared with HL stress alone. In addition to the induction of known stress-responsive genes, such as psbA, ocp and sodB, Nodularia cells activated the expression of genes coding for many previously unknown light- and oxidative stress-related proteins. In addition, the expression of non-protein-coding RNAs was found to be stimulated by these stresses. Among them was an antisense RNA to the phycocyanin-encoding mRNA cpcBAC and the trans-encoded regulator of photosystem I, PsrR1. The large genome capacity allowed Nodularia to harbor more copies of stress-relevant genes such as psbA and small chlorophyll-binding protein genes, combined with the coordinated induction of these and many additional genes for stress acclimation. Our data provide a first insight on how N. spumigena became adapted to conditions relevant for a cyanobacterial bloom in the Baltic Sea.The ISME Journal advance online publication, 17 February 2015; doi:10.1038/ismej.2015.16.

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Research paper thumbnail of 1: Pan Y, Ni R, Deng Q, Huang X, Zhang Y, Lu C, Li F, Huang D, He S, Chen B. Glyoxylate reductase/hydroxypyruvate reductase: a novel prognostic marker for hepatocellular carcinoma patients after curative resection. Pathobiology. 2013; 80 (3): 155-62. doi: 10.1159/000346476. Epub 2013 Mar 7. PubMe...

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Research paper thumbnail of Photorespiration - a driver for evolutionary innovations and key to better crops

Plant biology (Stuttgart, Germany), 2013

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Research paper thumbnail of Does the Cyanophora paradoxa genome revise our view on the evolution of photorespiratory enzymes?

Plant biology (Stuttgart, Germany), 2013

In the present-day O2 -rich atmosphere, the photorespiratory pathway is essential for organisms p... more In the present-day O2 -rich atmosphere, the photorespiratory pathway is essential for organisms performing oxygenic photosynthesis; i.e. cyanobacteria, algae and land plants. The presence of enzymes for the plant-like 2-phosphoglycolate cycle in cyanobacteria indicates that, together with oxygenic photosynthesis, genes for photorespiratory enzymes were endosymbiotically conveyed from ancient cyanobacteria to photosynthetic eukaryotes. The genome information for Cyanophora paradoxa, a member of the Glaucophyta representing the first branching group of primary endosymbionts, and for many other eukaryotic algae was used to shed light on the evolutionary relationship of photorespiratory enzymes among oxygenic phototrophs. For example, it became possible to analyse the phylogenies of 2-phosphoglycolate phosphatase, serine:glyoxylate aminotransferase and hydroxypyruvate reductase. Analysis of the Cyanophora genome provided clear evidence that some photorespiratory enzymes originally acquired from cyanobacteria were lost, e.g. glycerate 3-kinase, while others were replaced by the corresponding enzymes from the α-proteobacterial endosymbiont, e.g. serine:glyoxylate aminotransferase. Generally, our analysis supports the view that many C2 cycle enzymes in eukaryotic phototrophs were obtained from the cyanobacterial endosymbiont, but during the subsequent evolution of algae and land plants multiple losses and replacements occurred, which resulted in a reticulate provenance of photorespiratory enzymes with different origins in different cellular compartments.

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Research paper thumbnail of Nitric oxide, nitrate reductase and UV-B tolerance

Tree Physiology, 2011

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Research paper thumbnail of D-GLYCERATE 3-KINASE, the Last Unknown Enzyme in the Photorespiratory Cycle in Arabidopsis, Belongs to a Novel Kinase Family

THE PLANT CELL ONLINE, 2005

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Research paper thumbnail of Evolution of C4 Photosynthesis in the Genus Flaveria: How Many and Which Genes Does It Take to Make C4?

The Plant Cell, 2011

Selective pressure exerted by a massive decline in atmospheric CO(2) levels 55 to 40 million year... more Selective pressure exerted by a massive decline in atmospheric CO(2) levels 55 to 40 million years ago promoted the evolution of a novel, highly efficient mode of photosynthetic carbon assimilation known as C(4) photosynthesis. C(4) species have concurrently evolved multiple times in a broad range of plant families, and this multiple and parallel evolution of the complex C(4) trait indicates a common underlying evolutionary mechanism that might be elucidated by comparative analyses of related C(3) and C(4) species. Here, we use mRNA-Seq analysis of five species within the genus Flaveria, ranging from C(3) to C(3)-C(4) intermediate to C(4) species, to quantify the differences in the transcriptomes of closely related plant species with varying degrees of C(4)-associated characteristics. Single gene analysis defines the C(4) cycle enzymes and transporters more precisely and provides new candidates for yet unknown functions as well as identifies C(4) associated pathways. Molecular evidence for a photorespiratory CO(2) pump prior to the establishment of the C(4) cycle-based CO(2) pump is provided. Cluster analysis defines the upper limit of C(4)-related gene expression changes in mature leaves of Flaveria as 3582 alterations.

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Research paper thumbnail of Cyanobacterial Lactate Oxidases Serve as Essential Partners in N2 Fixation and Evolved into Photorespiratory Glycolate Oxidases in Plants

The Plant Cell, 2011

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Research paper thumbnail of The photorespiratory glycolate metabolism is essential for cyanobacteria and might have been conveyed endosymbiontically to plants

Proceedings of the National Academy of Sciences, 2008

Photorespiratory 2-phosphoglycolate (2PG) metabolism is essential for photosynthesis in higher pl... more Photorespiratory 2-phosphoglycolate (2PG) metabolism is essential for photosynthesis in higher plants but thought to be superfluous in cyanobacteria because of their ability to concentrate CO(2) internally and thereby inhibit photorespiration. Here, we show that 3 routes for 2PG metabolism are present in the model cyanobacterium Synechocystis sp. strain PCC 6803. In addition to the photorespiratory C2 cycle characterized in plants, this cyanobacterium also possesses the bacterial glycerate pathway and is able to completely decarboxylate glyoxylate via oxalate. A triple mutant with defects in all 3 routes of 2PG metabolism exhibited a high-CO(2)-requiring (HCR) phenotype. All these catabolic routes start with glyoxylate, which can be synthesized by 2 different forms of glycolate dehydrogenase (GlcD). Mutants defective in one or both GlcD proteins accumulated glycolate under high CO(2) level and the double mutant DeltaglcD1/DeltaglcD2 was unable to grow under low CO(2). The HCR phenotype of both the double and the triple mutant could not be attributed to a significantly reduced affinity to CO(2), such as in other cyanobacterial HCR mutants defective in the CO(2)-concentrating mechanism (CCM). These unexpected findings of an HCR phenotype in the presence of an active CCM indicate that 2PG metabolism is essential for the viability of all organisms that perform oxygenic photosynthesis, including cyanobacteria and C3 plants, at ambient CO(2) conditions. These data and phylogenetic analyses suggest cyanobacteria as the evolutionary origin not only of oxygenic photosynthesis but also of an ancient photorespiratory 2PG metabolism.

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Research paper thumbnail of Reassimilation of carbon dioxide by Flaveria (Asteraceae) species representing different types of photosynthesis

Planta, 1987

The capability to reassimilate CO2 originating from intracellular decarboxylating processes conne... more The capability to reassimilate CO2 originating from intracellular decarboxylating processes connected with the photorespiratory glycolate pathway and-or decarboxylation of C4 acids during C4 photosynthesis has been investigated with four species of the genus Flaveria (Asteraceae). The C3-C4 intermediate species F. pubescens and F. anomala reassimilated CO2 much more efficiently than the C3 species F. cronquistii and, with respect to this feature, behaved similarly to the C4 species F. trinervia. Therefore, under atmospheric conditions the intermediate species photorespired with rates only between 10-20% of that measured with F. cronquistii. At low oxygen concentrations (1,5%) the reassimilation potential of F. anomala approached that of F. trinervia and was distinct from that found with F. pubescens. The data are discussed with respect to a possible sequence of events during evolution of C4 photosynthesis. If compared with related data for C3-C4 intermediate species from other genera they support the hypothesis that, during evolution of C4 photosynthesis, an efficient capacity for CO2 reassimilation evolved prior to a CO2-concentrating mechanism.

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Research paper thumbnail of Photorespiratory 2-phosphoglycolate metabolism and photoreduction of O2 cooperate in high-light acclimation of Synechocystis sp. strain PCC 6803

Planta, 2009

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Research paper thumbnail of Metabolic response of potato plants to an antisense reduction of the P-protein of glycine decarboxylase

Planta, 2001

Potato (Solanum tuberosum L. cv. Desiré) plants with reduced amounts of P-protein, one of the sub... more Potato (Solanum tuberosum L. cv. Desiré) plants with reduced amounts of P-protein, one of the subunits of glycine decarboxylase (GDC), have been generated by introduction of an antisense transgene. Two transgenic lines, containing about 60-70% less P-protein in the leaves compared to wild-type potato, were analysed in more detail. The reduction in P-protein amount led to a decrease in the ability of leaf mitochondria to decarboxylate glycine. Photosynthetic and growth rates were reduced but the plants were viable under ambient air and produced tubers. Glycine concentrations within the leaves were elevated up to about 100-fold during illumination. Effects on other amino acids and on sucrose and hexoses were minor. Nearly all of the glycine accumulated during the day was metabolised during the following night. The data suggest that the GDC operates far below substrate saturation under normal conditions thus allowing a flexible and fast response to changes in the environment.

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Research paper thumbnail of The emerging roles of nitric oxide (NO) in plant mitochondria

Plant Science, 2011

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Research paper thumbnail of Metabolome Phenotyping of Inorganic Carbon Limitation in Cells of the Wild Type and Photorespiratory Mutants of the Cyanobacterium Synechocystis sp. Strain PCC 6803

PLANT PHYSIOLOGY, 2008

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Research paper thumbnail of The Gene for the P-Subunit of Glycine Decarboxylase from the C4 Species Flaveria trinervia: Analysis of Transcriptional Control in Transgenic Flaveria bidentis (C4) and Arabidopsis (C3)

PLANT PHYSIOLOGY, 2008

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Research paper thumbnail of Long-Term Response toward Inorganic Carbon Limitation in Wild Type and Glycolate Turnover Mutants of the Cyanobacterium Synechocystis sp. Strain PCC 6803

PLANT PHYSIOLOGY, 2007

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Research paper thumbnail of Arabidopsis Mutants with Strongly Reduced Levels of the T-Protein Subunit of Glycine Decarboxylase

Photosynthesis. Energy from the Sun, 2008

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Research paper thumbnail of Oxalate Decarboxylase is Involved in Turnover of 2-Phosphoglycolate in Synechocystis sp. Strain PCC 6803

Photosynthesis. Energy from the Sun, 2008

Despite the presence of a carbon concentrating mechanism, cyanobacteria synthesize 2- phosphoglyc... more Despite the presence of a carbon concentrating mechanism, cyanobacteria synthesize 2- phosphoglycolate (2PG) by the oxygenase reaction of Rubisco. Using DNA-microarray analyses, we examined the regulation of genes involved in 2PG metabolism in the cyanobacterium Synechocystis PCC 6803. While genes for the plant-like C2 cycle were barely influenced by low Ci conditions, genes of the bacterial-like glycerate pathway were upregulated.

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Research paper thumbnail of Lipoate-Protein Ligase and Octanoyltransferase Are Essential for Protein Lipoylation in Mitochondria of Arabidopsis

Plant physiology, Jan 28, 2014

Prosthetic lipoyl groups are required for the function of several essential multienzyme complexes... more Prosthetic lipoyl groups are required for the function of several essential multienzyme complexes, such as pyruvate dehydrogenase (PDH), α-ketoglutarate dehydrogenase (KGDH), and the glycine cleavage system (glycine decarboxylase [GDC]). How these proteins are lipoylated has been extensively studied in prokaryotes and yeast (Saccharomyces cerevisiae), but little is known for plants. We earlier reported that mitochondrial fatty acid synthesis by ketoacyl-acyl carrier protein synthase is not vital for protein lipoylation in Arabidopsis (Arabidopsis thaliana) and does not play a significant role in roots. Here, we identify Arabidopsis lipoate-protein ligase (AtLPLA) as an essential mitochondrial enzyme that uses octanoyl-nucleoside monophosphate and possibly other donor substrates for the octanoylation of mitochondrial PDH-E2 and GDC H-protein; it shows no reactivity with bacterial and possibly plant KGDH-E2. The octanoate-activating enzyme is unknown, but we assume that it uses octano...

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Research paper thumbnail of C2 photosynthesis generates about 3-fold elevated leaf CO2 levels in the C3-C4 intermediate species Flaveria pubescens

Journal of experimental botany, 2014

Formation of a photorespiration-based CO2-concentrating mechanism in C3-C4 intermediate plants is... more Formation of a photorespiration-based CO2-concentrating mechanism in C3-C4 intermediate plants is seen as a prerequisite for the evolution of C4 photosynthesis, but it is not known how efficient this mechanism is. Here, using in vivo Rubisco carboxylation-to-oxygenation ratios as a proxy to assess relative intraplastidial CO2 levels is suggested. Such ratios were determined for the C3-C4 intermediate species Flaveria pubescens compared with the closely related C3 plant F. cronquistii and the C4 plant F. trinervia. To this end, a model was developed to describe the major carbon fluxes and metabolite pools involved in photosynthetic-photorespiratory carbon metabolism and used quantitatively to evaluate the labelling kinetics during short-term (14)CO2 incorporation. Our data suggest that the photorespiratory CO2 pump elevates the intraplastidial CO2 concentration about 3-fold in leaves of the C3-C4 intermediate species F. pubescens relative to the C3 species F. cronquistii.

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Expression profiling of the bloom-forming cyanobacterium Nodularia CCY9414 under light and oxidative stress conditions

The ISME Journal, 2015

Massive blooms of toxic cyanobacteria frequently occur in the central Baltic Sea during the summe... more Massive blooms of toxic cyanobacteria frequently occur in the central Baltic Sea during the summer. In the surface scum, cyanobacterial cells are exposed to high light (HL) intensity, high oxygen partial pressure and other stresses. To mimic these conditions, cultures of Nodularia spumigena CCY9414, which is a strain isolated from a cyanobacterial summer bloom in the Baltic Sea, were incubated at a HL intensity of 1200 μmol photons m(-2) s(-1) or a combination of HL and increased oxygen partial pressure. Using differential RNA sequencing, we compared the global primary transcriptomes of control and stressed cells. The combination of oxidative and light stresses induced the expression of twofold more genes compared with HL stress alone. In addition to the induction of known stress-responsive genes, such as psbA, ocp and sodB, Nodularia cells activated the expression of genes coding for many previously unknown light- and oxidative stress-related proteins. In addition, the expression of non-protein-coding RNAs was found to be stimulated by these stresses. Among them was an antisense RNA to the phycocyanin-encoding mRNA cpcBAC and the trans-encoded regulator of photosystem I, PsrR1. The large genome capacity allowed Nodularia to harbor more copies of stress-relevant genes such as psbA and small chlorophyll-binding protein genes, combined with the coordinated induction of these and many additional genes for stress acclimation. Our data provide a first insight on how N. spumigena became adapted to conditions relevant for a cyanobacterial bloom in the Baltic Sea.The ISME Journal advance online publication, 17 February 2015; doi:10.1038/ismej.2015.16.

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Research paper thumbnail of 1: Pan Y, Ni R, Deng Q, Huang X, Zhang Y, Lu C, Li F, Huang D, He S, Chen B. Glyoxylate reductase/hydroxypyruvate reductase: a novel prognostic marker for hepatocellular carcinoma patients after curative resection. Pathobiology. 2013; 80 (3): 155-62. doi: 10.1159/000346476. Epub 2013 Mar 7. PubMe...

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Research paper thumbnail of Photorespiration - a driver for evolutionary innovations and key to better crops

Plant biology (Stuttgart, Germany), 2013

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Does the Cyanophora paradoxa genome revise our view on the evolution of photorespiratory enzymes?

Plant biology (Stuttgart, Germany), 2013

In the present-day O2 -rich atmosphere, the photorespiratory pathway is essential for organisms p... more In the present-day O2 -rich atmosphere, the photorespiratory pathway is essential for organisms performing oxygenic photosynthesis; i.e. cyanobacteria, algae and land plants. The presence of enzymes for the plant-like 2-phosphoglycolate cycle in cyanobacteria indicates that, together with oxygenic photosynthesis, genes for photorespiratory enzymes were endosymbiotically conveyed from ancient cyanobacteria to photosynthetic eukaryotes. The genome information for Cyanophora paradoxa, a member of the Glaucophyta representing the first branching group of primary endosymbionts, and for many other eukaryotic algae was used to shed light on the evolutionary relationship of photorespiratory enzymes among oxygenic phototrophs. For example, it became possible to analyse the phylogenies of 2-phosphoglycolate phosphatase, serine:glyoxylate aminotransferase and hydroxypyruvate reductase. Analysis of the Cyanophora genome provided clear evidence that some photorespiratory enzymes originally acquired from cyanobacteria were lost, e.g. glycerate 3-kinase, while others were replaced by the corresponding enzymes from the α-proteobacterial endosymbiont, e.g. serine:glyoxylate aminotransferase. Generally, our analysis supports the view that many C2 cycle enzymes in eukaryotic phototrophs were obtained from the cyanobacterial endosymbiont, but during the subsequent evolution of algae and land plants multiple losses and replacements occurred, which resulted in a reticulate provenance of photorespiratory enzymes with different origins in different cellular compartments.

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Research paper thumbnail of Nitric oxide, nitrate reductase and UV-B tolerance

Tree Physiology, 2011

Bookmarks Related papers MentionsView impact

Research paper thumbnail of D-GLYCERATE 3-KINASE, the Last Unknown Enzyme in the Photorespiratory Cycle in Arabidopsis, Belongs to a Novel Kinase Family

THE PLANT CELL ONLINE, 2005

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Evolution of C4 Photosynthesis in the Genus Flaveria: How Many and Which Genes Does It Take to Make C4?

The Plant Cell, 2011

Selective pressure exerted by a massive decline in atmospheric CO(2) levels 55 to 40 million year... more Selective pressure exerted by a massive decline in atmospheric CO(2) levels 55 to 40 million years ago promoted the evolution of a novel, highly efficient mode of photosynthetic carbon assimilation known as C(4) photosynthesis. C(4) species have concurrently evolved multiple times in a broad range of plant families, and this multiple and parallel evolution of the complex C(4) trait indicates a common underlying evolutionary mechanism that might be elucidated by comparative analyses of related C(3) and C(4) species. Here, we use mRNA-Seq analysis of five species within the genus Flaveria, ranging from C(3) to C(3)-C(4) intermediate to C(4) species, to quantify the differences in the transcriptomes of closely related plant species with varying degrees of C(4)-associated characteristics. Single gene analysis defines the C(4) cycle enzymes and transporters more precisely and provides new candidates for yet unknown functions as well as identifies C(4) associated pathways. Molecular evidence for a photorespiratory CO(2) pump prior to the establishment of the C(4) cycle-based CO(2) pump is provided. Cluster analysis defines the upper limit of C(4)-related gene expression changes in mature leaves of Flaveria as 3582 alterations.

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Research paper thumbnail of Cyanobacterial Lactate Oxidases Serve as Essential Partners in N2 Fixation and Evolved into Photorespiratory Glycolate Oxidases in Plants

The Plant Cell, 2011

Bookmarks Related papers MentionsView impact

Research paper thumbnail of The photorespiratory glycolate metabolism is essential for cyanobacteria and might have been conveyed endosymbiontically to plants

Proceedings of the National Academy of Sciences, 2008

Photorespiratory 2-phosphoglycolate (2PG) metabolism is essential for photosynthesis in higher pl... more Photorespiratory 2-phosphoglycolate (2PG) metabolism is essential for photosynthesis in higher plants but thought to be superfluous in cyanobacteria because of their ability to concentrate CO(2) internally and thereby inhibit photorespiration. Here, we show that 3 routes for 2PG metabolism are present in the model cyanobacterium Synechocystis sp. strain PCC 6803. In addition to the photorespiratory C2 cycle characterized in plants, this cyanobacterium also possesses the bacterial glycerate pathway and is able to completely decarboxylate glyoxylate via oxalate. A triple mutant with defects in all 3 routes of 2PG metabolism exhibited a high-CO(2)-requiring (HCR) phenotype. All these catabolic routes start with glyoxylate, which can be synthesized by 2 different forms of glycolate dehydrogenase (GlcD). Mutants defective in one or both GlcD proteins accumulated glycolate under high CO(2) level and the double mutant DeltaglcD1/DeltaglcD2 was unable to grow under low CO(2). The HCR phenotype of both the double and the triple mutant could not be attributed to a significantly reduced affinity to CO(2), such as in other cyanobacterial HCR mutants defective in the CO(2)-concentrating mechanism (CCM). These unexpected findings of an HCR phenotype in the presence of an active CCM indicate that 2PG metabolism is essential for the viability of all organisms that perform oxygenic photosynthesis, including cyanobacteria and C3 plants, at ambient CO(2) conditions. These data and phylogenetic analyses suggest cyanobacteria as the evolutionary origin not only of oxygenic photosynthesis but also of an ancient photorespiratory 2PG metabolism.

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Reassimilation of carbon dioxide by Flaveria (Asteraceae) species representing different types of photosynthesis

Planta, 1987

The capability to reassimilate CO2 originating from intracellular decarboxylating processes conne... more The capability to reassimilate CO2 originating from intracellular decarboxylating processes connected with the photorespiratory glycolate pathway and-or decarboxylation of C4 acids during C4 photosynthesis has been investigated with four species of the genus Flaveria (Asteraceae). The C3-C4 intermediate species F. pubescens and F. anomala reassimilated CO2 much more efficiently than the C3 species F. cronquistii and, with respect to this feature, behaved similarly to the C4 species F. trinervia. Therefore, under atmospheric conditions the intermediate species photorespired with rates only between 10-20% of that measured with F. cronquistii. At low oxygen concentrations (1,5%) the reassimilation potential of F. anomala approached that of F. trinervia and was distinct from that found with F. pubescens. The data are discussed with respect to a possible sequence of events during evolution of C4 photosynthesis. If compared with related data for C3-C4 intermediate species from other genera they support the hypothesis that, during evolution of C4 photosynthesis, an efficient capacity for CO2 reassimilation evolved prior to a CO2-concentrating mechanism.

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Photorespiratory 2-phosphoglycolate metabolism and photoreduction of O2 cooperate in high-light acclimation of Synechocystis sp. strain PCC 6803

Planta, 2009

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Metabolic response of potato plants to an antisense reduction of the P-protein of glycine decarboxylase

Planta, 2001

Potato (Solanum tuberosum L. cv. Desiré) plants with reduced amounts of P-protein, one of the sub... more Potato (Solanum tuberosum L. cv. Desiré) plants with reduced amounts of P-protein, one of the subunits of glycine decarboxylase (GDC), have been generated by introduction of an antisense transgene. Two transgenic lines, containing about 60-70% less P-protein in the leaves compared to wild-type potato, were analysed in more detail. The reduction in P-protein amount led to a decrease in the ability of leaf mitochondria to decarboxylate glycine. Photosynthetic and growth rates were reduced but the plants were viable under ambient air and produced tubers. Glycine concentrations within the leaves were elevated up to about 100-fold during illumination. Effects on other amino acids and on sucrose and hexoses were minor. Nearly all of the glycine accumulated during the day was metabolised during the following night. The data suggest that the GDC operates far below substrate saturation under normal conditions thus allowing a flexible and fast response to changes in the environment.

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Research paper thumbnail of The emerging roles of nitric oxide (NO) in plant mitochondria

Plant Science, 2011

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Research paper thumbnail of Metabolome Phenotyping of Inorganic Carbon Limitation in Cells of the Wild Type and Photorespiratory Mutants of the Cyanobacterium Synechocystis sp. Strain PCC 6803

PLANT PHYSIOLOGY, 2008

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Research paper thumbnail of The Gene for the P-Subunit of Glycine Decarboxylase from the C4 Species Flaveria trinervia: Analysis of Transcriptional Control in Transgenic Flaveria bidentis (C4) and Arabidopsis (C3)

PLANT PHYSIOLOGY, 2008

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Research paper thumbnail of Long-Term Response toward Inorganic Carbon Limitation in Wild Type and Glycolate Turnover Mutants of the Cyanobacterium Synechocystis sp. Strain PCC 6803

PLANT PHYSIOLOGY, 2007

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