Himanshu Misra - Academia.edu (original) (raw)

Papers by Himanshu Misra

High-performance thin-layer chromatography (HPTLC) has been used for normal-phase separation of t... more High-performance thin-layer chromatography (HPTLC) has been used for normal-phase separation of the components of hexane, chloroform, methanol, and water extracts of Rauvolfia serpentina roots. Computerized densitometry was used for two-dimensional spectrographic image ...

An attempt has been made to develop a method by which to determine the chemical fingerprint of An... more An attempt has been made to develop a method by which to determine the chemical fingerprint of Andrographis paniculata (Acanthaceae). High-performance thin layer chromatography (HPTLC) was used to analyse hexane, chloroform, methanol and water extracts of leaves of A. paniculata. A computerised densitometer was applied to the two-dimensional spectrographic image analysis of the HPTLC plates. An HPLC equipped with a photodiode array detector was used for the analyses of these different extracts. The analyses showed that andrographolide and neoandrographolide are absent in the hexane extract but are present in greater amounts in the methanol extract as compared with the other extracts. These chromatograms may serve as a chemical fingerprint of the drug A. paniculata for quality control purposes and in the preparation of formulations based on the drug.

Records of Natural Products

A simple, precise and accurate high-performance thin-layer chromatographic method has been establ... more A simple, precise and accurate high-performance thin-layer chromatographic method has been established for quantitative determination of quinine. Conditions were also optimized for best possible extraction of quinine via varying concentrations of diethyl amine in different solvents (n-hexane, chloroform, ethyl acetate and methanol) for maximum recovery of quinine. Methanol modified with 20 % DEA found to be best for highest possible recovery of target analyte quinine. Chromatographic separation of quinine was performed on silica gel 60 F 254 HPTLC plates with ethyl acetate : diethyl amine in the proportion 88 : 12 (v/v), as mobile phase. The determination was carried out using the densitometric absorbance mode at 236 nm. Quinine response was found to be linear over the range 4-24 µg spot −1 . The HPTLC method was evaluated in terms of specificity, precision, reproducibility, LOD -LOQ and robustness. Beside these parameters, number of theoretical plates and flow constant were also included as a part of validation.

Records of Natural Products

A simple, rapid and precise (%RSD<2.5) high-performance thin-layer chromatographic method was dev... more A simple, rapid and precise (%RSD<2.5) high-performance thin-layer chromatographic method was developed for quantitative estimation of a hepatoprotective diterpenoid andrographolide. Separation was performed on 60 F254 HPTLC plates using chloroform : methanol :: 27 : 3 v/v as mobile phase for elution of extract components. The determination was carried out in ultra-violet mode using densitometric absorbance-reflection mode at 232 nm. The concentration of andrographolide in the whole plant powder was 0.178 (±0.003) %, dry weight basis. Andrographolide response was found to be linear over a broad range 100 -2500 ng spot -1 . Limit of detection and quantification were 40ng and 100ng spot -1 . The developed method is capable of quantifying smaller as well as higher amounts of andrographolide in its plant raw-material. The HPTLC method was evaluated in terms of precision, accuracy, sensitivity and robustness.

Journal of Saudi Chemical Society, 2014

Column chromatography of purified butanol extract obtained after fractionation from aqueous metha... more Column chromatography of purified butanol extract obtained after fractionation from aqueous methanolic extract of aerial parts of Swertia paniculata afforded one xanthone glycoside (1,5-dihydroxy-3-methoxyxanthone-8-O-b-D-glucopyranoside). Structure of this compound was elucidated using spectroscopic techniques. This is the first report of isolation of this compound from S. paniculata. ª 2011 Production and hosting by Elsevier B.V. on behalf of King Saud University. * Corresponding author. Address: 15, Senior MIG, Vikram Nagar, Ratlam 457 001,

Chromatography Research International, 2014

A simple, rapid, precise, and accurate high-performance thin-layer chromatographic method coupled... more A simple, rapid, precise, and accurate high-performance thin-layer chromatographic method coupled with visible densitometric detection of artemisinin is developed and validated. Samples of the dried Artemisia annua leaves were extracted via microwaves using different solvents. This method shows the advantage of shorter extraction time of artemisinin from leaves under the influence of electromagnetic radiations. Results obtained from microwave-assisted extraction (MAE) were compared with hot soxhlet extraction. Chromatographic separation of artemisinin from plant extract was performed over silica gel 60 F 254 HPTLC plate using n-hexane : ethyl acetate as mobile phase in the ratio of 75 : 25, v/v. The plate was developed at room temperature 25 ± 2.0 ∘ C. Artemisinin separation over thin-layer plate was visualized after postchromatographic derivatization with anisaldehyde-sulphuric acid reagent. HPTLC plate was scanned in a CAMAG's TLC scanner 3 at 540 nm. Artemisinin responses were found to be linear over a range of 400-2800 ng spot −1 with a correlation coefficient 0.99754. Limits of detection and quantification were 40 and 80 ng spot −1 , respectively. The HPTLC method was validated in terms of system suitability, precision, accuracy, sensitivity (LOD and LOQ), and robustness. Additionally, calculation of plate efficiency and flow constant were included as components of validation. Extracts prepared from different parts of the plant (leaves, branches, main stem, and roots) were analyzed for artemisinin content, in which, artemisinin content was found higher in the leaf extract with respect to branches and main stem extracts; however, no artemisinin was detected in root extract. The developed HPTLC-visible method of artemisinin determination will be very useful for pharmaceutical industries, which are involved in monitoring of artemisinin content during different growth stages (in vitro and in vivo) of A. annua for qualitative and quantitative assessment of final produce prior to commercial-scale processing for assessment of cost-benefit ratio. chloroquine, mefloquine, and pyrimethamine, but malaria parasite developed resistance to these drugs [1-3]. Artemisinin ) is a natural product obtained from an aromatic annual herb of Asian and East European origin Artemisia annua L., which belongs to the family Asteraceae (Compositae). Due to the problem of resistance, artemisinin and its semisynthetic derivatives artemether, arteether, and artesunate are considered to be the most effective for the

Arabian Journal of Chemistry, 2012

Novel lipids were isolated from the unsaponifiable matter extracted from leaves of Artabotrys odo... more Novel lipids were isolated from the unsaponifiable matter extracted from leaves of Artabotrys odoratissimus (R.Br) by using n-hexane. The two known compounds, one new carboxyester and two monoesters were the new lipids identified by spectral (IR, 1 H NMR, 13 C NMR spectra, mass spectrum, elemental analysis) and chemical analysis. The carboxyester was identified as 1-carboxy-heneicosane pentadecanoate and two monoesters were identified as hexyl pentaicosanote and pentyl pentaicosanoate. Antimicrobial activities were assessed for all extracts and isolated compounds and showed variable activity. These are novel compounds and being reported first time by us.

Organic Chemistry International, 2013

Journal of Pharmacy and Bioallied Sciences, 2011

International Journal of Green Pharmacy, 2009

Arabian Journal of Chemistry

Three phytochemicals, curcumin 1, demethoxycurcumin 2 and b-sitosterol-3-O-b-Dglucopyranoside 3 h... more Three phytochemicals, curcumin 1, demethoxycurcumin 2 and b-sitosterol-3-O-b-Dglucopyranoside 3 have been isolated from the ethyl acetate extract of rhizomes of Curcuma aromatica. Chemical structures of all the three isolates were determined using spectroscopic and chemical analyses. b-Sitosterol-3-O-b-D-glucopyranoside has been isolated for the first time from this plant.

Jpc-journal of Planar Chromatography-modern Tlc, 2006

High-performance thin-layer chromatography (HPTLC) has been used for normal-phase separation of t... more High-performance thin-layer chromatography (HPTLC) has been used for normal-phase separation of the components of hexane, chloroform, methanol, and water extracts of Rauvolfia serpentina roots. Computerized densitometry was used for two-dimensional spectrographic image ...

Phytochemical Analysis, 2004

An attempt has been made to develop a method by which to determine the chemical fingerprint of And... more An attempt has been made to develop a method by which to determine the chemical fingerprint of Andrographis paniculata (Acanthaceae). High-performance thin layer chromatography (HPTLC) was used to analyse hexane, chloroform, methanol and water extracts of leaves of A. paniculata. A computerised densitometer was applied to the two-dimensional spectrographic image analysis of the HPTLC plates. An HPLC equipped with a photodiode array detector was used for the analyses of these different extracts. The analyses showed that andrographolide and neoandrographolide are absent in the hexane extract but are present in greater amounts in the methanol extract as compared with the other extracts. These chromatograms may serve as a chemical fingerprint of the drug A. paniculata for quality control purposes and in the preparation of formulations based on the drug. Copyright © 2004 John Wiley & Sons, Ltd.

High-performance thin-layer chromatography (HPTLC) has been used for normal-phase separation of t... more High-performance thin-layer chromatography (HPTLC) has been used for normal-phase separation of the components of hexane, chloroform, methanol, and water extracts of Rauvolfia serpentina roots. Computerized densitometry was used for two-dimensional spectrographic image ...

An attempt has been made to develop a method by which to determine the chemical fingerprint of An... more An attempt has been made to develop a method by which to determine the chemical fingerprint of Andrographis paniculata (Acanthaceae). High-performance thin layer chromatography (HPTLC) was used to analyse hexane, chloroform, methanol and water extracts of leaves of A. paniculata. A computerised densitometer was applied to the two-dimensional spectrographic image analysis of the HPTLC plates. An HPLC equipped with a photodiode array detector was used for the analyses of these different extracts. The analyses showed that andrographolide and neoandrographolide are absent in the hexane extract but are present in greater amounts in the methanol extract as compared with the other extracts. These chromatograms may serve as a chemical fingerprint of the drug A. paniculata for quality control purposes and in the preparation of formulations based on the drug.

Records of Natural Products

A simple, precise and accurate high-performance thin-layer chromatographic method has been establ... more A simple, precise and accurate high-performance thin-layer chromatographic method has been established for quantitative determination of quinine. Conditions were also optimized for best possible extraction of quinine via varying concentrations of diethyl amine in different solvents (n-hexane, chloroform, ethyl acetate and methanol) for maximum recovery of quinine. Methanol modified with 20 % DEA found to be best for highest possible recovery of target analyte quinine. Chromatographic separation of quinine was performed on silica gel 60 F 254 HPTLC plates with ethyl acetate : diethyl amine in the proportion 88 : 12 (v/v), as mobile phase. The determination was carried out using the densitometric absorbance mode at 236 nm. Quinine response was found to be linear over the range 4-24 µg spot −1 . The HPTLC method was evaluated in terms of specificity, precision, reproducibility, LOD -LOQ and robustness. Beside these parameters, number of theoretical plates and flow constant were also included as a part of validation.

Records of Natural Products

A simple, rapid and precise (%RSD<2.5) high-performance thin-layer chromatographic method was dev... more A simple, rapid and precise (%RSD<2.5) high-performance thin-layer chromatographic method was developed for quantitative estimation of a hepatoprotective diterpenoid andrographolide. Separation was performed on 60 F254 HPTLC plates using chloroform : methanol :: 27 : 3 v/v as mobile phase for elution of extract components. The determination was carried out in ultra-violet mode using densitometric absorbance-reflection mode at 232 nm. The concentration of andrographolide in the whole plant powder was 0.178 (±0.003) %, dry weight basis. Andrographolide response was found to be linear over a broad range 100 -2500 ng spot -1 . Limit of detection and quantification were 40ng and 100ng spot -1 . The developed method is capable of quantifying smaller as well as higher amounts of andrographolide in its plant raw-material. The HPTLC method was evaluated in terms of precision, accuracy, sensitivity and robustness.

Journal of Saudi Chemical Society, 2014

Column chromatography of purified butanol extract obtained after fractionation from aqueous metha... more Column chromatography of purified butanol extract obtained after fractionation from aqueous methanolic extract of aerial parts of Swertia paniculata afforded one xanthone glycoside (1,5-dihydroxy-3-methoxyxanthone-8-O-b-D-glucopyranoside). Structure of this compound was elucidated using spectroscopic techniques. This is the first report of isolation of this compound from S. paniculata. ª 2011 Production and hosting by Elsevier B.V. on behalf of King Saud University. * Corresponding author. Address: 15, Senior MIG, Vikram Nagar, Ratlam 457 001,

Chromatography Research International, 2014

A simple, rapid, precise, and accurate high-performance thin-layer chromatographic method coupled... more A simple, rapid, precise, and accurate high-performance thin-layer chromatographic method coupled with visible densitometric detection of artemisinin is developed and validated. Samples of the dried Artemisia annua leaves were extracted via microwaves using different solvents. This method shows the advantage of shorter extraction time of artemisinin from leaves under the influence of electromagnetic radiations. Results obtained from microwave-assisted extraction (MAE) were compared with hot soxhlet extraction. Chromatographic separation of artemisinin from plant extract was performed over silica gel 60 F 254 HPTLC plate using n-hexane : ethyl acetate as mobile phase in the ratio of 75 : 25, v/v. The plate was developed at room temperature 25 ± 2.0 ∘ C. Artemisinin separation over thin-layer plate was visualized after postchromatographic derivatization with anisaldehyde-sulphuric acid reagent. HPTLC plate was scanned in a CAMAG's TLC scanner 3 at 540 nm. Artemisinin responses were found to be linear over a range of 400-2800 ng spot −1 with a correlation coefficient 0.99754. Limits of detection and quantification were 40 and 80 ng spot −1 , respectively. The HPTLC method was validated in terms of system suitability, precision, accuracy, sensitivity (LOD and LOQ), and robustness. Additionally, calculation of plate efficiency and flow constant were included as components of validation. Extracts prepared from different parts of the plant (leaves, branches, main stem, and roots) were analyzed for artemisinin content, in which, artemisinin content was found higher in the leaf extract with respect to branches and main stem extracts; however, no artemisinin was detected in root extract. The developed HPTLC-visible method of artemisinin determination will be very useful for pharmaceutical industries, which are involved in monitoring of artemisinin content during different growth stages (in vitro and in vivo) of A. annua for qualitative and quantitative assessment of final produce prior to commercial-scale processing for assessment of cost-benefit ratio. chloroquine, mefloquine, and pyrimethamine, but malaria parasite developed resistance to these drugs [1-3]. Artemisinin ) is a natural product obtained from an aromatic annual herb of Asian and East European origin Artemisia annua L., which belongs to the family Asteraceae (Compositae). Due to the problem of resistance, artemisinin and its semisynthetic derivatives artemether, arteether, and artesunate are considered to be the most effective for the

Arabian Journal of Chemistry, 2012

Novel lipids were isolated from the unsaponifiable matter extracted from leaves of Artabotrys odo... more Novel lipids were isolated from the unsaponifiable matter extracted from leaves of Artabotrys odoratissimus (R.Br) by using n-hexane. The two known compounds, one new carboxyester and two monoesters were the new lipids identified by spectral (IR, 1 H NMR, 13 C NMR spectra, mass spectrum, elemental analysis) and chemical analysis. The carboxyester was identified as 1-carboxy-heneicosane pentadecanoate and two monoesters were identified as hexyl pentaicosanote and pentyl pentaicosanoate. Antimicrobial activities were assessed for all extracts and isolated compounds and showed variable activity. These are novel compounds and being reported first time by us.

Organic Chemistry International, 2013

Journal of Pharmacy and Bioallied Sciences, 2011

International Journal of Green Pharmacy, 2009

Arabian Journal of Chemistry

Three phytochemicals, curcumin 1, demethoxycurcumin 2 and b-sitosterol-3-O-b-Dglucopyranoside 3 h... more Three phytochemicals, curcumin 1, demethoxycurcumin 2 and b-sitosterol-3-O-b-Dglucopyranoside 3 have been isolated from the ethyl acetate extract of rhizomes of Curcuma aromatica. Chemical structures of all the three isolates were determined using spectroscopic and chemical analyses. b-Sitosterol-3-O-b-D-glucopyranoside has been isolated for the first time from this plant.

Jpc-journal of Planar Chromatography-modern Tlc, 2006

High-performance thin-layer chromatography (HPTLC) has been used for normal-phase separation of t... more High-performance thin-layer chromatography (HPTLC) has been used for normal-phase separation of the components of hexane, chloroform, methanol, and water extracts of Rauvolfia serpentina roots. Computerized densitometry was used for two-dimensional spectrographic image ...

Phytochemical Analysis, 2004

An attempt has been made to develop a method by which to determine the chemical fingerprint of And... more An attempt has been made to develop a method by which to determine the chemical fingerprint of Andrographis paniculata (Acanthaceae). High-performance thin layer chromatography (HPTLC) was used to analyse hexane, chloroform, methanol and water extracts of leaves of A. paniculata. A computerised densitometer was applied to the two-dimensional spectrographic image analysis of the HPTLC plates. An HPLC equipped with a photodiode array detector was used for the analyses of these different extracts. The analyses showed that andrographolide and neoandrographolide are absent in the hexane extract but are present in greater amounts in the methanol extract as compared with the other extracts. These chromatograms may serve as a chemical fingerprint of the drug A. paniculata for quality control purposes and in the preparation of formulations based on the drug. Copyright © 2004 John Wiley & Sons, Ltd.