H. Oudadesse - Academia.edu (original) (raw)

Papers by H. Oudadesse

Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 1998

The PIXE method is very suitable for applications in the biological field. We use it to study the... more The PIXE method is very suitable for applications in the biological field. We use it to study the kinetic resorption of bioceramics. The aim of this work is to compare the concentrations of mineral elements in bone to those in bioceramics. The samples are embedded in a polymethylmethacrylate resin and are considered as infinitely thick targets. The correction method is

Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 2002

Abstract Bioactive glasses are used as coating biomaterials to facilitate anchorage of metallic p... more Abstract Bioactive glasses are used as coating biomaterials to facilitate anchorage of metallic prostheses implanted into the body. The aim of this work is to study the behavior of gel formed in contact with alloys and BVA and BVH bioactive glasses implanted. Cylinders of metallic implants composed by Ti, Al and V, are coated with bioactive glass. Three sheep were implanted for different time length: 3, 6 and 12 months in the femoral epiphysis. Results obtained with particle induced X-ray emission and scanning electron microscopy show that BVA coating induces a better contact between the metallic implant and bone. On the other hand, BVH coating prevents corrosion from the metallic implant.

Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 1994

ABSTRACT Biomaterials are frequently implanted in bones. This implantation is followed by a pheno... more ABSTRACT Biomaterials are frequently implanted in bones. This implantation is followed by a phenomenon of ossification. The purpose of this work was to study the time evolution of the gradient of characteristic atomic element's concentrations in the bone, the implant and the bone-implant interface. We have studied two types of neutral biomaterials: pure synthetic hydroxyapatite and porite's asteroid coral. The animal implantations have been made on sheep of the same age and sex having received the same basic diet. The implantations have been made in the cortical femur. On both sides of the implant, at the same distance, two screws were placed to allow further determination of the position of the implant. The PIXE method is particularly suitable here because of the possibility to analyze directly the samples without any preparation and to choose easily the dimensions of beam used for the gradient study. The X-rays have been detected with an ultra LEGe instead of the usual Si(Li) device to avoid the Si escape peak associated with the Kalpha X-ray of calcium, the major constituent of bone. This peak is particularly disturbing here because its energy corresponds to the Kalpha line of phosphorus, an important constituent of bone. The results of these determinations are presented and discussed.

Journal of Radioanalytical and Nuclear Chemistry Articles, 1993

Coral has ben used for the last ten years as bone substitution in the body because of its mechani... more Coral has ben used for the last ten years as bone substitution in the body because of its mechanical an osteoconductor properties. Our primary studies have shown, for the first time, the quantitative behaviorofthe atomic components. We study by some physicalmethod of analysis a biocoral implanted "in vivo". The natural biocorals used in this work are the calcium carbonated exoskeletons built by Madrepian coral polyps. Neutron activation analysis showed that initial coral, essentially CaCO3, becomes a new material which has a mineral composition close to that of bone. In this work, we have studied the calcification mechanism of this implant by using radioactive tracers. We have established the transfer kinetics of calcium biomaterial in the blood circuit and showed its use by the organism for skeleton mineralization. Coral may be used as a substitution biomaterial in bone grafts. Its physico-chemical and architectural properties seem to be adapted to the biological requirements of the receiver bone. The use of physical analysis methods, such as neutron radioactivation and X-ray diffraction, show that the coral, after its implantation "in vivo", reaches a mineral composition and crystalline structure comparable to those of a bone. 1,2,5 In this work, we have studied the calcification mechanism of this implant by using radioactive tracers. The originality of this method consists in analyzing the atomic elements contained in the initial coral as a function of time spent in the body, by marking the calcium and the strontium contained in its structure.

Biomaterials, 1996

Coral and hydroxyapatite may be used as substitution biomaterials for bone grafts. In this work, ... more Coral and hydroxyapatite may be used as substitution biomaterials for bone grafts. In this work, we extracted the implants from the femora to study the kinetics of elementary mineral transformation of the osseous substitutes. The use of physical analysis methods such as PIXE (particle-induced X-ray emission) shows that coral and hydroxyapatite, after their implantation in vivo, reach a mineral composition comparable with that of bone. For the first time we have measured the concentration of mineral elements, at different time intervals after implantation, along a cross-section. The distribution according to mineral elements (Ca, P, Sr, Zn, Fe) in the implant, in the receiver site and also at the interface, showed that the kinetics of coral resorption was faster than that of hydroxyapatite; in the same way, the osseous attack was not global but, rather, centripetal.

Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 2004

Proton induced X-ray emission was used for a multi-element analysis of three biomaterials implant... more Proton induced X-ray emission was used for a multi-element analysis of three biomaterials implanted in ovine thighbone: one tri-tricalcic phosphate and two calcium carbonates with different percentages of porosity. The proton PIXE analysis method allowed the simultaneous quantification of several elements to get profiles of concentrations versus implantation delay. We focused on calcium and phosphorous which were major elements of the bony matrix and strontium, a trace element characteristic of the mineralization of the implants. Concentrations of calcium (constitutive of CaCO 3 and TCP) and phosphorous (constitutive of TCP) were known and differed from the bony matrix concentrations. The analysis of each sample for various times after implantation (1, 3, 6 and 12 months) by PIXE permitted to observe the evolution of the concentration of Ca, P and Sr in the implant area and at the interface implant/bone and to compare the different biomaterials implanted.

Ceramics International, 2014

Scaffolds are implants used to deliver cells, drugs, and genes into the body in a local controlle... more Scaffolds are implants used to deliver cells, drugs, and genes into the body in a local controlled release pattern which offers many advantages over systematic drug delivery. Composite scaffolds of polyvinyl alcohol (PVA) and quaternary bioactive glass (46S6 system) with different ratios of glass contents were prepared by lyophilisation technique. The broad spectrum antibiotic ciprofloxacin (Cip) was impregnated to the scaffold during the fabrication in a concentration of 5, 10 and 20%. Biodegradation rate and in-vitro mineralization of the prepared scaffolds were performed by soaking the scaffolds in simulated body fluid (SBF). Phase identification, microstructure, porosity, bioactivity, mechanical properties and drug release pattern in PBS were characterized by XRD, SEM coupled with EDS, Hg-porosimeter, inductively coupled plasma-optical emission spectroscopy (ICP-OES), universal testing machine, fourier transform infrared (FTIR) and UV-spectrophotometer, respectively. A porous scaffold has been obtained with porosity up to 85%. By increasing the glass contents in the prepared scaffold the porosity and the degradation rate decrease however, the compressive strength was enhanced. A sustained drug release pattern was observed with a quasi-Fickian diffusion mechanism. The formulated ciprofloxacin loaded porous polyvinyl alcohol scaffold gave an acceptable physicochemical properties and was able to deliver the drug in a prolonged release pattern which offers a distinguish treatment for osteomylitis as well as local antibacterial effect.

Bioceramics Development and Applications, 2011

Many types of bone substitutes exist to fill a bony defect. They must be compatible and bond with... more Many types of bone substitutes exist to fill a bony defect. They must be compatible and bond with the host tissue without any formation of fibrous capsule. The direct apposition of bone to bioactive materials, including bioactive glasses and calcium phosphates, has already been demonstrated. In this work, different compositions of bioactive glasses elaborated by melting and rapid quenching were studied. They were based on SiO 2-CaO-Na 2 O and P 2 O 5. Several physicochemical methods like XRD, FTIR, SEM, and ICP-OES were employed to characterize all bioactive glasses. They were investigated as bony biomaterials using "in vitro" assays. The aim was to evaluate their chemical reactivity and their bioactivity after soaking in simulated body fluid (SBF).

Biological Trace Element Research, 1999

Prostheses can release some metallic elements to the surrounding tissues, particularly when they ... more Prostheses can release some metallic elements to the surrounding tissues, particularly when they are not covered with a biomaterial layer and when an unsealing process happens. We try to measure major and trace elements in these tissues with an experimentally sensitive method. Proton-induced X-ray emission is used to detect about 10 elements in tissue. Tissues are calcinated and deposited in a thin layer before irradiation. Results are obtained in a standard and samples from three patients. We observe contamination by Ti, Cr, Ni, and Zn in the tissues. Correlations are to be studied between these atomic transfers and prosthesis in the patient.

Annales Pharmaceutiques Françaises, 2014

Recently, the therapeutic approaches of the detoxification against the metals (nickel) in the bod... more Recently, the therapeutic approaches of the detoxification against the metals (nickel) in the body are the use of biomaterials such as carbonated hydroxyapatite. The aim of this study is therefore to analyze the physiological and physicochemical parameters of strain white rats "Wistar" receiving nickel chloride and to study the protective associative of apatite against adverse effects of this metal, and this in comparison with control rats. Our results showed that the nickel induced in rats an oxidative stress objectified by elevated levels of thiobarbituric acid-reactive substances and conjugated dienes associated with inhibition of the activity of the antioxidant defense system such as glutathione peroxidase, superoxide dismutase and catalase in the liver, kidney, spleen and erythrocyte. Disorders balances of ferric, phosphocalcic, a renal failure and a liver toxicity were observed in rats exposed to nickel. As well as a significant increase in the rate of nickel in the bones and microcytic anemia was revealed. However, the implantation of carbonated hydroxyapatite in capsule form protects rats intoxicated by the nickel against the toxic effects of this metal by lowering the levels of markers of lipid peroxidation and improving the activities of defense enzymes. Our implantation technique is effective to correct ferric balance and phosphocalcic equilibrium, to protect liver and kidney function, to reduce the rate of bone nickel and to correct anemia. They clearly explain the beneficial and protective of our biomaterial which aims the detoxification of rats receiving nickel by substituting cationic (Ca(2+) by Ni(2+)) and anionic (OH(-) by Cl(-)) confirmed by physicochemical characterization like the IR spectroscopy and X-ray diffraction. These techniques have shown on the one hand a duplication of OH(-) bands (IR) and on the other hand the increase of the volume of the apatite cell after these substitutions (X-ray diffraction).

Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 2002

Some metallic prostheses inserted in human hip undergo physico-chemical modification, a few years... more Some metallic prostheses inserted in human hip undergo physico-chemical modification, a few years after their implantation. Tissues surrounding these prostheses are damaged by metallic element transfer. Surgeons in Clermont-Ferrand Hospital (France) recover tissues of abnormal coloration that were in contact with metallic implants. PIXE technique (particles induced X-ray emission) with a 400 lm proton beam and 3 MeV of energy is an efficient technique to analyze these tissues and to detect elements, which are transferred from prosthesis to tissues. PIXE analyses were carried at the CERI-CNRS Laboratory. We have applied this method to determine qualitatively and quantitatively trace elements migration from metallic implants to surrounding tissues and organs, like kidney, spleen, liver, lymphatic gland and lung. Ó

Journal of Thermal Analysis and Calorimetry, 2000

A new type of aluminosilicate matrix calcium phosphate crystallites composites (ACPC) was synthes... more A new type of aluminosilicate matrix calcium phosphate crystallites composites (ACPC) was synthesized and studied for osseous bone applications. The room temperature synthesis of the aluminosilicate matrix and composites was described. Thermal treatments of compounds allowed the adaptability of some parameters (pH, porosity and mechanical properties). Structure of heat treated composites were characterized by XRD and FTIR. The influence of thermal treatment on the mechanical properties, the porosity and the pH was studied for two temperatures (250 and 500°C). Results evidenced the ability to control the pH, the high level of porosity (≈70%) and the good mechanical properties, allowing to consider that ACPC are potential biomaterials for osseous bone application.

Journal of Biomaterials Science, Polymer Edition, 1996

Substituting the tissue of human organs with biomaterials is problematic. However, its importance... more Substituting the tissue of human organs with biomaterials is problematic. However, its importance and relevance justify all the efforts made. An interdisciplinary approach is required. We report on our study of a product for bone substitution. Coral is a natural product, the interest of which we have already demonstrated in our previous work. Following sterilization, natural coral was implanted in sheep femurs. We regularly extracted the implants from the femurs to study the kinetics of elemental mineral transformation of the bone substitutes. For the first time ever, and thanks to the PIXE method (particles induced X-ray emission), we were able to measure the concentration of mineral elements at different time intervals after implantation over a whole cross-section. We found a discontinuity of the mineral elements (Ca, P, Sr, Zn, Fe) at the interface between the implant and the receiver. This shows that the osseous attack is not global but, on the contrary, centripetal. Moreover, the fit of the concentration time course indicates that the kinetics of ossification are different for each atomic element and characterize a distinct biological phenomenon. Our analyses confirm the biocompatibility and the ossification of the implanted coral.

Journal of Applied Physics, 2001

The European Physical Journal Applied Physics, 2007

The purpose of this work is to study the formation of hydroxyapatite (Ca{10} (PO{4})6(OH){2}) on ... more The purpose of this work is to study the formation of hydroxyapatite (Ca{10} (PO{4})6(OH){2}) on the surface of glass 48S4 with chemical composition: SiO{2}: 48%, CaO: 30%, Na{2}O: 18% and P{2}O{5}: 4% in weight ratio. This selected composition presents phosphorus contributions lower than that in BioglasscircledR [Hench et al. J. Biomed. Mater. 36, 117 (1971)] developed by L. Hench. Comparison

Bioceramics Development and Applications

Different bioactive glass systems have been prepared by sol-gel. However, the production of Na2O-... more Different bioactive glass systems have been prepared by sol-gel. However, the production of Na2O-containing bioactive glasses by sol–gel methods has proved to be difficult as the sodium nitrate used in the preparation could be lost from the glass structure during filtration and washing. The aim of this study was to prepare the quaternary system 46S6 of bioactive glass by modified sol-gel techniques with a decrease in the time of gelation. In addition, compare the behaviour of the prepared sol-gel bioactive glass system by its corresponding prepared by melting. The obtained glasses were characterized by using several physicochemical techniques; XRD, FTIR, TEM and SEM beside the effect of the glass particles on the viability of osteoblast like cells (Saos-2). Results show that nanopowders 40-60 nm of 46S6 glass system had been prepared by modified sol-gel (acid-base reaction) method at 600°C in just three days at 600°C. Cell viability by MTT assay confirmed the effectiveness of the pr...

Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 1998

The PIXE method is very suitable for applications in the biological field. We use it to study the... more The PIXE method is very suitable for applications in the biological field. We use it to study the kinetic resorption of bioceramics. The aim of this work is to compare the concentrations of mineral elements in bone to those in bioceramics. The samples are embedded in a polymethylmethacrylate resin and are considered as infinitely thick targets. The correction method is

Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 2002

Abstract Bioactive glasses are used as coating biomaterials to facilitate anchorage of metallic p... more Abstract Bioactive glasses are used as coating biomaterials to facilitate anchorage of metallic prostheses implanted into the body. The aim of this work is to study the behavior of gel formed in contact with alloys and BVA and BVH bioactive glasses implanted. Cylinders of metallic implants composed by Ti, Al and V, are coated with bioactive glass. Three sheep were implanted for different time length: 3, 6 and 12 months in the femoral epiphysis. Results obtained with particle induced X-ray emission and scanning electron microscopy show that BVA coating induces a better contact between the metallic implant and bone. On the other hand, BVH coating prevents corrosion from the metallic implant.

Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 1994

ABSTRACT Biomaterials are frequently implanted in bones. This implantation is followed by a pheno... more ABSTRACT Biomaterials are frequently implanted in bones. This implantation is followed by a phenomenon of ossification. The purpose of this work was to study the time evolution of the gradient of characteristic atomic element's concentrations in the bone, the implant and the bone-implant interface. We have studied two types of neutral biomaterials: pure synthetic hydroxyapatite and porite's asteroid coral. The animal implantations have been made on sheep of the same age and sex having received the same basic diet. The implantations have been made in the cortical femur. On both sides of the implant, at the same distance, two screws were placed to allow further determination of the position of the implant. The PIXE method is particularly suitable here because of the possibility to analyze directly the samples without any preparation and to choose easily the dimensions of beam used for the gradient study. The X-rays have been detected with an ultra LEGe instead of the usual Si(Li) device to avoid the Si escape peak associated with the Kalpha X-ray of calcium, the major constituent of bone. This peak is particularly disturbing here because its energy corresponds to the Kalpha line of phosphorus, an important constituent of bone. The results of these determinations are presented and discussed.

Journal of Radioanalytical and Nuclear Chemistry Articles, 1993

Coral has ben used for the last ten years as bone substitution in the body because of its mechani... more Coral has ben used for the last ten years as bone substitution in the body because of its mechanical an osteoconductor properties. Our primary studies have shown, for the first time, the quantitative behaviorofthe atomic components. We study by some physicalmethod of analysis a biocoral implanted "in vivo". The natural biocorals used in this work are the calcium carbonated exoskeletons built by Madrepian coral polyps. Neutron activation analysis showed that initial coral, essentially CaCO3, becomes a new material which has a mineral composition close to that of bone. In this work, we have studied the calcification mechanism of this implant by using radioactive tracers. We have established the transfer kinetics of calcium biomaterial in the blood circuit and showed its use by the organism for skeleton mineralization. Coral may be used as a substitution biomaterial in bone grafts. Its physico-chemical and architectural properties seem to be adapted to the biological requirements of the receiver bone. The use of physical analysis methods, such as neutron radioactivation and X-ray diffraction, show that the coral, after its implantation "in vivo", reaches a mineral composition and crystalline structure comparable to those of a bone. 1,2,5 In this work, we have studied the calcification mechanism of this implant by using radioactive tracers. The originality of this method consists in analyzing the atomic elements contained in the initial coral as a function of time spent in the body, by marking the calcium and the strontium contained in its structure.

Biomaterials, 1996

Coral and hydroxyapatite may be used as substitution biomaterials for bone grafts. In this work, ... more Coral and hydroxyapatite may be used as substitution biomaterials for bone grafts. In this work, we extracted the implants from the femora to study the kinetics of elementary mineral transformation of the osseous substitutes. The use of physical analysis methods such as PIXE (particle-induced X-ray emission) shows that coral and hydroxyapatite, after their implantation in vivo, reach a mineral composition comparable with that of bone. For the first time we have measured the concentration of mineral elements, at different time intervals after implantation, along a cross-section. The distribution according to mineral elements (Ca, P, Sr, Zn, Fe) in the implant, in the receiver site and also at the interface, showed that the kinetics of coral resorption was faster than that of hydroxyapatite; in the same way, the osseous attack was not global but, rather, centripetal.

Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 2004

Proton induced X-ray emission was used for a multi-element analysis of three biomaterials implant... more Proton induced X-ray emission was used for a multi-element analysis of three biomaterials implanted in ovine thighbone: one tri-tricalcic phosphate and two calcium carbonates with different percentages of porosity. The proton PIXE analysis method allowed the simultaneous quantification of several elements to get profiles of concentrations versus implantation delay. We focused on calcium and phosphorous which were major elements of the bony matrix and strontium, a trace element characteristic of the mineralization of the implants. Concentrations of calcium (constitutive of CaCO 3 and TCP) and phosphorous (constitutive of TCP) were known and differed from the bony matrix concentrations. The analysis of each sample for various times after implantation (1, 3, 6 and 12 months) by PIXE permitted to observe the evolution of the concentration of Ca, P and Sr in the implant area and at the interface implant/bone and to compare the different biomaterials implanted.

Ceramics International, 2014

Scaffolds are implants used to deliver cells, drugs, and genes into the body in a local controlle... more Scaffolds are implants used to deliver cells, drugs, and genes into the body in a local controlled release pattern which offers many advantages over systematic drug delivery. Composite scaffolds of polyvinyl alcohol (PVA) and quaternary bioactive glass (46S6 system) with different ratios of glass contents were prepared by lyophilisation technique. The broad spectrum antibiotic ciprofloxacin (Cip) was impregnated to the scaffold during the fabrication in a concentration of 5, 10 and 20%. Biodegradation rate and in-vitro mineralization of the prepared scaffolds were performed by soaking the scaffolds in simulated body fluid (SBF). Phase identification, microstructure, porosity, bioactivity, mechanical properties and drug release pattern in PBS were characterized by XRD, SEM coupled with EDS, Hg-porosimeter, inductively coupled plasma-optical emission spectroscopy (ICP-OES), universal testing machine, fourier transform infrared (FTIR) and UV-spectrophotometer, respectively. A porous scaffold has been obtained with porosity up to 85%. By increasing the glass contents in the prepared scaffold the porosity and the degradation rate decrease however, the compressive strength was enhanced. A sustained drug release pattern was observed with a quasi-Fickian diffusion mechanism. The formulated ciprofloxacin loaded porous polyvinyl alcohol scaffold gave an acceptable physicochemical properties and was able to deliver the drug in a prolonged release pattern which offers a distinguish treatment for osteomylitis as well as local antibacterial effect.

Bioceramics Development and Applications, 2011

Many types of bone substitutes exist to fill a bony defect. They must be compatible and bond with... more Many types of bone substitutes exist to fill a bony defect. They must be compatible and bond with the host tissue without any formation of fibrous capsule. The direct apposition of bone to bioactive materials, including bioactive glasses and calcium phosphates, has already been demonstrated. In this work, different compositions of bioactive glasses elaborated by melting and rapid quenching were studied. They were based on SiO 2-CaO-Na 2 O and P 2 O 5. Several physicochemical methods like XRD, FTIR, SEM, and ICP-OES were employed to characterize all bioactive glasses. They were investigated as bony biomaterials using "in vitro" assays. The aim was to evaluate their chemical reactivity and their bioactivity after soaking in simulated body fluid (SBF).

Biological Trace Element Research, 1999

Prostheses can release some metallic elements to the surrounding tissues, particularly when they ... more Prostheses can release some metallic elements to the surrounding tissues, particularly when they are not covered with a biomaterial layer and when an unsealing process happens. We try to measure major and trace elements in these tissues with an experimentally sensitive method. Proton-induced X-ray emission is used to detect about 10 elements in tissue. Tissues are calcinated and deposited in a thin layer before irradiation. Results are obtained in a standard and samples from three patients. We observe contamination by Ti, Cr, Ni, and Zn in the tissues. Correlations are to be studied between these atomic transfers and prosthesis in the patient.

Annales Pharmaceutiques Françaises, 2014

Recently, the therapeutic approaches of the detoxification against the metals (nickel) in the bod... more Recently, the therapeutic approaches of the detoxification against the metals (nickel) in the body are the use of biomaterials such as carbonated hydroxyapatite. The aim of this study is therefore to analyze the physiological and physicochemical parameters of strain white rats "Wistar" receiving nickel chloride and to study the protective associative of apatite against adverse effects of this metal, and this in comparison with control rats. Our results showed that the nickel induced in rats an oxidative stress objectified by elevated levels of thiobarbituric acid-reactive substances and conjugated dienes associated with inhibition of the activity of the antioxidant defense system such as glutathione peroxidase, superoxide dismutase and catalase in the liver, kidney, spleen and erythrocyte. Disorders balances of ferric, phosphocalcic, a renal failure and a liver toxicity were observed in rats exposed to nickel. As well as a significant increase in the rate of nickel in the bones and microcytic anemia was revealed. However, the implantation of carbonated hydroxyapatite in capsule form protects rats intoxicated by the nickel against the toxic effects of this metal by lowering the levels of markers of lipid peroxidation and improving the activities of defense enzymes. Our implantation technique is effective to correct ferric balance and phosphocalcic equilibrium, to protect liver and kidney function, to reduce the rate of bone nickel and to correct anemia. They clearly explain the beneficial and protective of our biomaterial which aims the detoxification of rats receiving nickel by substituting cationic (Ca(2+) by Ni(2+)) and anionic (OH(-) by Cl(-)) confirmed by physicochemical characterization like the IR spectroscopy and X-ray diffraction. These techniques have shown on the one hand a duplication of OH(-) bands (IR) and on the other hand the increase of the volume of the apatite cell after these substitutions (X-ray diffraction).

Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 2002

Some metallic prostheses inserted in human hip undergo physico-chemical modification, a few years... more Some metallic prostheses inserted in human hip undergo physico-chemical modification, a few years after their implantation. Tissues surrounding these prostheses are damaged by metallic element transfer. Surgeons in Clermont-Ferrand Hospital (France) recover tissues of abnormal coloration that were in contact with metallic implants. PIXE technique (particles induced X-ray emission) with a 400 lm proton beam and 3 MeV of energy is an efficient technique to analyze these tissues and to detect elements, which are transferred from prosthesis to tissues. PIXE analyses were carried at the CERI-CNRS Laboratory. We have applied this method to determine qualitatively and quantitatively trace elements migration from metallic implants to surrounding tissues and organs, like kidney, spleen, liver, lymphatic gland and lung. Ó

Journal of Thermal Analysis and Calorimetry, 2000

A new type of aluminosilicate matrix calcium phosphate crystallites composites (ACPC) was synthes... more A new type of aluminosilicate matrix calcium phosphate crystallites composites (ACPC) was synthesized and studied for osseous bone applications. The room temperature synthesis of the aluminosilicate matrix and composites was described. Thermal treatments of compounds allowed the adaptability of some parameters (pH, porosity and mechanical properties). Structure of heat treated composites were characterized by XRD and FTIR. The influence of thermal treatment on the mechanical properties, the porosity and the pH was studied for two temperatures (250 and 500°C). Results evidenced the ability to control the pH, the high level of porosity (≈70%) and the good mechanical properties, allowing to consider that ACPC are potential biomaterials for osseous bone application.

Journal of Biomaterials Science, Polymer Edition, 1996

Substituting the tissue of human organs with biomaterials is problematic. However, its importance... more Substituting the tissue of human organs with biomaterials is problematic. However, its importance and relevance justify all the efforts made. An interdisciplinary approach is required. We report on our study of a product for bone substitution. Coral is a natural product, the interest of which we have already demonstrated in our previous work. Following sterilization, natural coral was implanted in sheep femurs. We regularly extracted the implants from the femurs to study the kinetics of elemental mineral transformation of the bone substitutes. For the first time ever, and thanks to the PIXE method (particles induced X-ray emission), we were able to measure the concentration of mineral elements at different time intervals after implantation over a whole cross-section. We found a discontinuity of the mineral elements (Ca, P, Sr, Zn, Fe) at the interface between the implant and the receiver. This shows that the osseous attack is not global but, on the contrary, centripetal. Moreover, the fit of the concentration time course indicates that the kinetics of ossification are different for each atomic element and characterize a distinct biological phenomenon. Our analyses confirm the biocompatibility and the ossification of the implanted coral.

Journal of Applied Physics, 2001

The European Physical Journal Applied Physics, 2007

The purpose of this work is to study the formation of hydroxyapatite (Ca{10} (PO{4})6(OH){2}) on ... more The purpose of this work is to study the formation of hydroxyapatite (Ca{10} (PO{4})6(OH){2}) on the surface of glass 48S4 with chemical composition: SiO{2}: 48%, CaO: 30%, Na{2}O: 18% and P{2}O{5}: 4% in weight ratio. This selected composition presents phosphorus contributions lower than that in BioglasscircledR [Hench et al. J. Biomed. Mater. 36, 117 (1971)] developed by L. Hench. Comparison

Bioceramics Development and Applications

Different bioactive glass systems have been prepared by sol-gel. However, the production of Na2O-... more Different bioactive glass systems have been prepared by sol-gel. However, the production of Na2O-containing bioactive glasses by sol–gel methods has proved to be difficult as the sodium nitrate used in the preparation could be lost from the glass structure during filtration and washing. The aim of this study was to prepare the quaternary system 46S6 of bioactive glass by modified sol-gel techniques with a decrease in the time of gelation. In addition, compare the behaviour of the prepared sol-gel bioactive glass system by its corresponding prepared by melting. The obtained glasses were characterized by using several physicochemical techniques; XRD, FTIR, TEM and SEM beside the effect of the glass particles on the viability of osteoblast like cells (Saos-2). Results show that nanopowders 40-60 nm of 46S6 glass system had been prepared by modified sol-gel (acid-base reaction) method at 600°C in just three days at 600°C. Cell viability by MTT assay confirmed the effectiveness of the pr...