Harry Parathon - Academia.edu (original) (raw)

Papers by Harry Parathon

Research paper thumbnail of An Accumulation of Cesium Radionuclides in Forest Food Production

Research paper thumbnail of Effect of Amniotic Membrane-Derived Mesenchymal Stem Cells on TNF-? Expression and Inflammatory Cells Infiltration during Vesicovaginal Fistule Repair Healing Process

Indian Journal of Forensic Medicine & Toxicology, 2020

Background: Vesicovaginal fistula (VVF) causes high morbidity in women, affecting both physical a... more Background: Vesicovaginal fistula (VVF) causes high morbidity in women, affecting both physical and psychological condition. Until now, surgery is the mainstay treatment for this condition. However, prolonged exposure during inflammatory phase after surgery is still a problem in the healing process. TNF-α as potent pro-inflammatory cytokine plays an important role by attracting inflammatory cells to wound tissue. Amniotic membrane is the source for mesenchymal stem cells that had anti-inflammatory and imunomodulatory effect. This study aims to evaluate the effect of Amniotic Membrane-Derived Mesenchymal Stem Cells (AMMSC) on TNF-α expression and inflammatory cell infiltration during VVF repair healing process in New Zealand White (NZW) rabbit model. Method: This study was an experimental study with randomized posttest only control group design. Twentyseven NZW rabbit as VVF model was used in this study, randomly divided into 3 different treatment groups after underwent surgical trea...

Research paper thumbnail of Prospective identification by neonatal screening of patients with guanidinoacetate methyltransferase deficiency

Molecular Genetics and Metabolism, 2021

INTRODUCTION Guanidinoacetate methyltransferase (GAMT) deficiency is an inherited metabolic disor... more INTRODUCTION Guanidinoacetate methyltransferase (GAMT) deficiency is an inherited metabolic disorder that impairs the synthesis of creatine (CRE). Lack of CRE in the brain can cause intellectual disability, autistic-like behavior, seizures, and movement disorders. Identification at birth and immediate therapy can prevent intellectual disability and seizures. Here we report the first two cases of GAMT deficiency identified at birth by newborn screening (NBS) in Utah and New York. METHODS NBS dried blood spots were analyzed by tandem mass spectrometry (MS/MS) using either derivatized or non-derivatized assays to detect guanidinoacetate (GUAC) and CRE. For any positive samples, a second-tier test using a more selective method, ultra-performance liquid chromatography (UPLC) combined with MS/MS, was performed to separate GUAC from potential isobaric interferences. RESULTS NBS for GAMT deficiency began in Utah on June 1, 2015 using a derivatized method for the detection of GUAC and CRE. In May 2019, the laboratory and method transitioned to a non-derivatized method. GAMT screening was added to the New York State NBS panel on October 1, 2018 using a derivatized method. In New York, a total of 537,408 babies were screened, 23 infants were referred and one newborn was identified with GAMT deficiency. In Utah, a total of 273,902 infants were screened (195,425 with the derivatized method, 78,477 with the non-derivatized method), three infants referred and one was identified with GAMT deficiency. Mean levels of GUAC and CRE were similar between methods (Utah derivatized: GUAC = 1.20 ± 0.43 μmol/L, CRE = 238 ± 96 μmol/L; Utah non-derivatized: GUAC = 1.23 ± 0.61 μmol/L, CRE = 344 ± 150 μmol/L, New York derivatized: GUAC = 1.34 ± 0.57 μmol/L, CRE = 569 ± 155 μmol/L). With either Utah method, similar concentrations of GUAC are observed in first (collected around 1 day of age) and the second NBS specimens (routinely collected at 7-16 days of age), while CRE concentrations decreased in the second NBS specimens. Both infants identified with GAMT deficiency started therapy by 2 weeks of age and are growing and developing normally at 7 (Utah) and 4 (New York) months of age. CONCLUSIONS Newborn screening allows for the prospective identification of GAMT deficiency utilizing elevated GUAC concentration as a marker. First-tier screening may be incorporated into existing methods for amino acids and acylcarnitines without the need for new equipment or staff. Newborn screening performed by either derivatized or non-derivatized methods and coupled with second-tier testing, has a very low false positive rate and can prospectively identify affected children. SummaryCerebral creatine deficiency syndromes caused by defects in creatine synthesis can result in intellectual disability, and are preventable if therapy is initiated early in life. This manuscript reports the identification of two infants with GAMT deficiency (one of the cerebral creatine deficiency syndromes) by newborn screening and demonstrates NBS feasibility using a variety of methods.

Research paper thumbnail of Incidence of Bacterial Vagniosis and Aerobic Vaginitis before and after Pessary Insertion in Indonesia

Indian Journal of Public Health Research & Development, 2019

Research paper thumbnail of Urogynaecology

Journal of Obstetrics and Gynaecology Research, 2017

Introduction: Female genital congenital anomalies tend to increase. These anomalies may lead to a... more Introduction: Female genital congenital anomalies tend to increase. These anomalies may lead to abnormalities of menstrual and reproductive function even ambiguous social identity. Prevalence of female genital congenital anomalies in population was 4-7 %. Yet, there is still lacking of data in our center about these anomalies. Objective: Our study aimed to determine incidence, socio-demographic characteristics, type and therapy of female congenital genitalia anomaly patients in gynecology outpatient clinic Dr Soetomo General Hospital Surabaya Indonesia. Methods: This cross-sectional study was conducted to 80 cases of female congenital genitalia anomaly in Dr Soetomo General Hospital Surabaya Indonesia from January 2013 to December 2016. Data was showed descriptively. Results: We found 80 cases of female congenital genitalia anomaly from 3082 gynecology outpatient clinic within 4 years. The mean age of patients was 19.73 ± 9.37 year old. The mean age of menarche was 14.28 ± 2.02 year old. The mean marital age was 23.72 ± 4.75 years old. Sixty-two percent of patients were not yet married. The most cases were vaginal agenesis (30 %), followed by the vaginal septum (25 %), hymen imperforate (8.75 %), clitoromegaly (8.75 %) and others (27.5 %). Most of the cases were managed by surgical therapy (82.5 %) majority transvaginal surgery (87.8 %) with the most commonly used was neovagina with 2 technique amnion and sigmoid (25 %). Two cases suffered recurrent after surgical treatment then we did second surgical therapy, hysterectomy. Bleeding as the complication of therapy was only in 3 % of patients. Conclusions: Female congenital genitalia anomalies at Dr Soetomo General Hospital were dominated by vaginal agenesis with the most therapy used was neovagina with 2 technique amnion and sigmoid. There were two recurrent cases and three percent minimal bleeding complication.

Research paper thumbnail of Progress towards antimicrobial resistance containment and control in Indonesia

Research paper thumbnail of Third Grade Uterine Prolapse in Young Age after Second Surgical Reconstruction

Obstetrics & Gynecology International Journal, 2015

Research paper thumbnail of Prevalence and determinants of inappropriate antibiotic dispensing at private drug retail outlets in urban and rural areas of Indonesia: a mixed methods study

BMJ Global Health

IntroductionThe aim of this mixed-method study was to determine the extent and determinants of in... more IntroductionThe aim of this mixed-method study was to determine the extent and determinants of inappropriate dispensing of antibiotics by licensed private drug retail outlets in Indonesia.MethodsStandardised patients (SPs) made a total of 495 visits to 166 drug outlets (community pharmacies and drug stores) between July and August 2019. The SPs presented three clinical cases to drug outlet staff: parent of a child at home with diarrhoea; an adult with presumptive tuberculosis (TB); and an adult with upper respiratory tract infection (URTI). The primary outcome was the dispensing of an antibiotic without prescription, with or without the client requesting it. We used multivariable random effects logistic regression to assess factors associated with the primary outcome and conducted 31 interviews with drug outlet staff to explore these factors in greater depth.ResultsAntibiotic dispensing without prescription occurred in 69% of SP visits. Dispensing antibiotics without a prescription ...

Research paper thumbnail of An Accumulation of Cesium Radionuclides in Forest Food Production

Research paper thumbnail of Effect of Amniotic Membrane-Derived Mesenchymal Stem Cells on TNF-? Expression and Inflammatory Cells Infiltration during Vesicovaginal Fistule Repair Healing Process

Indian Journal of Forensic Medicine & Toxicology, 2020

Background: Vesicovaginal fistula (VVF) causes high morbidity in women, affecting both physical a... more Background: Vesicovaginal fistula (VVF) causes high morbidity in women, affecting both physical and psychological condition. Until now, surgery is the mainstay treatment for this condition. However, prolonged exposure during inflammatory phase after surgery is still a problem in the healing process. TNF-α as potent pro-inflammatory cytokine plays an important role by attracting inflammatory cells to wound tissue. Amniotic membrane is the source for mesenchymal stem cells that had anti-inflammatory and imunomodulatory effect. This study aims to evaluate the effect of Amniotic Membrane-Derived Mesenchymal Stem Cells (AMMSC) on TNF-α expression and inflammatory cell infiltration during VVF repair healing process in New Zealand White (NZW) rabbit model. Method: This study was an experimental study with randomized posttest only control group design. Twentyseven NZW rabbit as VVF model was used in this study, randomly divided into 3 different treatment groups after underwent surgical trea...

Research paper thumbnail of Prospective identification by neonatal screening of patients with guanidinoacetate methyltransferase deficiency

Molecular Genetics and Metabolism, 2021

INTRODUCTION Guanidinoacetate methyltransferase (GAMT) deficiency is an inherited metabolic disor... more INTRODUCTION Guanidinoacetate methyltransferase (GAMT) deficiency is an inherited metabolic disorder that impairs the synthesis of creatine (CRE). Lack of CRE in the brain can cause intellectual disability, autistic-like behavior, seizures, and movement disorders. Identification at birth and immediate therapy can prevent intellectual disability and seizures. Here we report the first two cases of GAMT deficiency identified at birth by newborn screening (NBS) in Utah and New York. METHODS NBS dried blood spots were analyzed by tandem mass spectrometry (MS/MS) using either derivatized or non-derivatized assays to detect guanidinoacetate (GUAC) and CRE. For any positive samples, a second-tier test using a more selective method, ultra-performance liquid chromatography (UPLC) combined with MS/MS, was performed to separate GUAC from potential isobaric interferences. RESULTS NBS for GAMT deficiency began in Utah on June 1, 2015 using a derivatized method for the detection of GUAC and CRE. In May 2019, the laboratory and method transitioned to a non-derivatized method. GAMT screening was added to the New York State NBS panel on October 1, 2018 using a derivatized method. In New York, a total of 537,408 babies were screened, 23 infants were referred and one newborn was identified with GAMT deficiency. In Utah, a total of 273,902 infants were screened (195,425 with the derivatized method, 78,477 with the non-derivatized method), three infants referred and one was identified with GAMT deficiency. Mean levels of GUAC and CRE were similar between methods (Utah derivatized: GUAC = 1.20 ± 0.43 μmol/L, CRE = 238 ± 96 μmol/L; Utah non-derivatized: GUAC = 1.23 ± 0.61 μmol/L, CRE = 344 ± 150 μmol/L, New York derivatized: GUAC = 1.34 ± 0.57 μmol/L, CRE = 569 ± 155 μmol/L). With either Utah method, similar concentrations of GUAC are observed in first (collected around 1 day of age) and the second NBS specimens (routinely collected at 7-16 days of age), while CRE concentrations decreased in the second NBS specimens. Both infants identified with GAMT deficiency started therapy by 2 weeks of age and are growing and developing normally at 7 (Utah) and 4 (New York) months of age. CONCLUSIONS Newborn screening allows for the prospective identification of GAMT deficiency utilizing elevated GUAC concentration as a marker. First-tier screening may be incorporated into existing methods for amino acids and acylcarnitines without the need for new equipment or staff. Newborn screening performed by either derivatized or non-derivatized methods and coupled with second-tier testing, has a very low false positive rate and can prospectively identify affected children. SummaryCerebral creatine deficiency syndromes caused by defects in creatine synthesis can result in intellectual disability, and are preventable if therapy is initiated early in life. This manuscript reports the identification of two infants with GAMT deficiency (one of the cerebral creatine deficiency syndromes) by newborn screening and demonstrates NBS feasibility using a variety of methods.

Research paper thumbnail of Incidence of Bacterial Vagniosis and Aerobic Vaginitis before and after Pessary Insertion in Indonesia

Indian Journal of Public Health Research & Development, 2019

Research paper thumbnail of Urogynaecology

Journal of Obstetrics and Gynaecology Research, 2017

Introduction: Female genital congenital anomalies tend to increase. These anomalies may lead to a... more Introduction: Female genital congenital anomalies tend to increase. These anomalies may lead to abnormalities of menstrual and reproductive function even ambiguous social identity. Prevalence of female genital congenital anomalies in population was 4-7 %. Yet, there is still lacking of data in our center about these anomalies. Objective: Our study aimed to determine incidence, socio-demographic characteristics, type and therapy of female congenital genitalia anomaly patients in gynecology outpatient clinic Dr Soetomo General Hospital Surabaya Indonesia. Methods: This cross-sectional study was conducted to 80 cases of female congenital genitalia anomaly in Dr Soetomo General Hospital Surabaya Indonesia from January 2013 to December 2016. Data was showed descriptively. Results: We found 80 cases of female congenital genitalia anomaly from 3082 gynecology outpatient clinic within 4 years. The mean age of patients was 19.73 ± 9.37 year old. The mean age of menarche was 14.28 ± 2.02 year old. The mean marital age was 23.72 ± 4.75 years old. Sixty-two percent of patients were not yet married. The most cases were vaginal agenesis (30 %), followed by the vaginal septum (25 %), hymen imperforate (8.75 %), clitoromegaly (8.75 %) and others (27.5 %). Most of the cases were managed by surgical therapy (82.5 %) majority transvaginal surgery (87.8 %) with the most commonly used was neovagina with 2 technique amnion and sigmoid (25 %). Two cases suffered recurrent after surgical treatment then we did second surgical therapy, hysterectomy. Bleeding as the complication of therapy was only in 3 % of patients. Conclusions: Female congenital genitalia anomalies at Dr Soetomo General Hospital were dominated by vaginal agenesis with the most therapy used was neovagina with 2 technique amnion and sigmoid. There were two recurrent cases and three percent minimal bleeding complication.

Research paper thumbnail of Progress towards antimicrobial resistance containment and control in Indonesia

Research paper thumbnail of Third Grade Uterine Prolapse in Young Age after Second Surgical Reconstruction

Obstetrics & Gynecology International Journal, 2015

Research paper thumbnail of Prevalence and determinants of inappropriate antibiotic dispensing at private drug retail outlets in urban and rural areas of Indonesia: a mixed methods study

BMJ Global Health

IntroductionThe aim of this mixed-method study was to determine the extent and determinants of in... more IntroductionThe aim of this mixed-method study was to determine the extent and determinants of inappropriate dispensing of antibiotics by licensed private drug retail outlets in Indonesia.MethodsStandardised patients (SPs) made a total of 495 visits to 166 drug outlets (community pharmacies and drug stores) between July and August 2019. The SPs presented three clinical cases to drug outlet staff: parent of a child at home with diarrhoea; an adult with presumptive tuberculosis (TB); and an adult with upper respiratory tract infection (URTI). The primary outcome was the dispensing of an antibiotic without prescription, with or without the client requesting it. We used multivariable random effects logistic regression to assess factors associated with the primary outcome and conducted 31 interviews with drug outlet staff to explore these factors in greater depth.ResultsAntibiotic dispensing without prescription occurred in 69% of SP visits. Dispensing antibiotics without a prescription ...