H. Rotaru - Academia.edu (original) (raw)
Papers by H. Rotaru
Lasers in Medical Science
International Journal of Oral and Maxillofacial Surgery, 2009
fibula in reconstruction of mandibular defects. Methods: Twenty patients complaining of massive m... more fibula in reconstruction of mandibular defects. Methods: Twenty patients complaining of massive mandibular lesions were enrolled in this study. Group I (ten patients) were reconstructed by non-vascularised iliac crest and rib. Group II (ten patients) were reconstructed by vascularised fibula. Follow-up has extended up to the third postoperative year. Results and Conclusion: Microvascular fibula transfer proved to be a treatment of choice for reconstruction of long mandibular defects with simultaneous soft tissue covering and irradiated bone. Nonvascularised bone grafts are suitable for short defects, non-irradiated bone with a good vascular bed.
International Journal of Oral and Maxillofacial Surgery, 2007
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie, 2011
Osteonecrosis of the jaws is increasing worldwide in patients treated with bisphosphonates. A ret... more Osteonecrosis of the jaws is increasing worldwide in patients treated with bisphosphonates. A retrospective review of 52 patients who were treated during 2007-2010 for bisphosphonate related maxillofacial symptoms of the jaws was conducted. Patient characteristics and other factors that influenced the disease process were studied. Thirteen patients received bisphosphonates for the prevention or treatment of osteoporosis; 39 for preventing bone metastases from malignant tumors. Thirty-six patients were females (age range 32-87 years, median 64 years); 16 were males (age range 30-81 years, median 73.5 years). Bisphosphonate used was ibandronic acid in four cases, alendronate sodium in 14 cases, and zoledronic acid in 34 cases. Mean bisphosphonate treatment period was 22.44 months (95%CI 19.33-25.55). Thirty patients received intravenous, 22 received oral bisphosphonate. The average period until occurrence of maxillofacial symptoms was 6 months (range 0.5-24 months) in subjects with in...
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie, 2011
Lengthening the mandible by distraction osteogenesis (DO) is nowadays a well-recognized technique... more Lengthening the mandible by distraction osteogenesis (DO) is nowadays a well-recognized technique in maxillofacial surgery. This study compared two different distraction rates and evaluated histological and histomorphometrical properties of the distracted bone in an experimental ovine mandible model with the goal of elaborating a universally accepted distraction protocol. Tissue blocks of regenerated bone were harvested from twelve young adult sheep. DO was performed on the mandibular midline after five days of latency period. The sheep were divided into two groups. The first group underwent activation of 0.8 mm÷day during 12 days resulting in 9.6 mm of new bone while the second group followed a geometric rate pattern of 0.2 mm - three days, 0.4 mm - three days, 0.8 mm - three days and 1.6 mm - three days resulting in 9 mm of new bone. The regenerated bone was histologically and histomorphometrically analyzed after 30, 45 and 60 days of consolidation. The relative osteoid volume (OV...
Journal of Cranio-Maxillofacial Surgery, 2008
Objectives: To evaluate in preclinical models (in vitro and in animal model) all requests of Good... more Objectives: To evaluate in preclinical models (in vitro and in animal model) all requests of Good Manufacturing Practice's rules before the clinical application of mesenchymal stem cells (MSC), in order to elaborate the protocols for using human autologous MSC in oral regenerative and maxillofacial medicine. Methods: MSC lies in bone marrow an other tissues; they are autologous cells that have not immunological reaction and are expandable in vitro. MSC are taken from bone marrow sample from healthy donor. After medium procedures, an omogeneous SMC colony is reached and its osteoblast differenziating capacity was tested with or without bone morphogenetic protein-7 (BMP-7). Four medium were used (standard medium, growth medium, osteogenic medium, osteogenic medium with BMP-7). Results: The expression of osteogenic genes are analized by polimerase chain reaction (PCR): alkaline phosphatase (ALP), osteopontin (SPP1), osteocalcin (BGLAP). In the osteogenic medium with BMP-7, it was observed an increase of ALP, involved in the early phase of the osteogenetic differenziation, as well as an increase of SPP1 and BGLAP, involved in the late phase of osteogenetic differenziation and mineralization. Conclusions: Results suggest that BMP-7 amplifies the osteogenic differenziation process. These results are present in the quality, efficency and safety protocol: "maxillo-facial surgery bone defects by using autologous stem cells expansed in vitro.
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie, 2013
The objectives of this study were to test the biocompatibility and to evaluate the osseointegrati... more The objectives of this study were to test the biocompatibility and to evaluate the osseointegration of Titanium-Aluminum-Niobium (Ti6Al7Nb) alloy used in the manufacturing of personalized implants with selective laser melting (SLM) technology and to compare the growth viability of osteoblastic-like cells on different Ti6Al7Nb alloy samples (plain, coated with hydroxyapatite or SiO2-TiO2) implanted into the cranial bone of Wistar rats. In terms of biocompatibility, the cone-beam computer-tomography head scans taken at the moment of sacrifice of each group (one, two and three months) showed no implant displacement, no osteolysis and no liquid collection around the implants. At one month, around all types of implants new bone formation was noticed, although around the plain Ti6Al7Nb implant a large amount of powder debris was present. Still, no inflammatory reaction was seen. At two months, the distance between the implants and the calvarial bone margins diminished. A thin layer of fib...
Particulate Science and Technology
International Journal of Oral and Maxillofacial Surgery, 2005
Revista medico-chirurgicală̆ a Societă̆ţ̜ii de Medici ş̧i Naturaliş̧ti din Iaş̧i
Life quality has a major importance in the actual social context. The evaluation af the pain sens... more Life quality has a major importance in the actual social context. The evaluation af the pain sensation as a stress major factor is difficult regarding the variety of the methods, their relevance and their compliance. We've made an evaluation concerning the trigeminal essential neuralgia pain using different tipe of parameters. The results obtained proved a scientific and clinical value of the method that we've initiated and applied in these research.
Revista medico-chirurgicală̆ a Societă̆ţ̜ii de Medici ş̧i Naturaliş̧ti din Iaş̧i
Arterio-venous vascular malformations (AVM) rarely occur in craniofacial bones. The most frequent... more Arterio-venous vascular malformations (AVM) rarely occur in craniofacial bones. The most frequent location has been reported in the mandible. The first clinical evidence of the lesions can be a fatal hemorrhage, occurring spontaneously or subsequent to dental therapy. Lack of knowledge about these lesions among specialists dealing with the oral and maxillofacial regions can lead to fatal complications. The study presents the successful multidisciplinary management of two cases of mandibular AVM using embolization, sclerotherapy and bone reconstruction, a major challenge owing to the substantial risk of morbidity, recurrence by recanalization and revascularization, neurologic deficits and mortality. There were no complications over a follow-up period of 12 years.
Revista medico-chirurgicală̆ a Societă̆ţ̜ii de Medici ş̧i Naturaliş̧ti din Iaş̧i
This case report highlights the possibility of a non extraction orthodontic treatment, surgically... more This case report highlights the possibility of a non extraction orthodontic treatment, surgically assisted, in a Class II Division 1 malocclusions in order to reduce maxillary protrusion prior to the surgical advancement of the mandible for the correction of the skeletal deficiency. The combined orthodontic-surgical approach optimizes the profile while allowing the settling of a Angle Class I occlusion at the end of the entire treatment sequence.
Revista medico-chirurgicală̆ a Societă̆ţ̜ii de Medici ş̧i Naturaliş̧ti din Iaş̧i
The interdisciplinary, complex therapeutic protocol of the cleft lip and palate patients, applied... more The interdisciplinary, complex therapeutic protocol of the cleft lip and palate patients, applied in the Clinic of Cranio-Maxillo-Facial Surgery of "I. Haţieganu" University of Medicine and Pharmacy Cluj-Napoca, involves the morphologic reconstruction as well as the functional rehabilitation. Functional rehabilitation is the aspect, which gives the esthetics, social and familial integration of the patient, offering good quality of life. In the current study, a new method and concept of improving the phonetic function in the primary and secondary surgical steps, with the effect on muscle and bone, is presented. The new surgical techniques used comprise of the surgery of the levator veli palatini using the method designed by Sader, and bone distraction, during the same surgical procedure. The assessment of the phonetic results was performed using the NARSOM test. Following up the results of the techniques mentioned above, we consider that they improve extremely well the morp...
International Journal of Oral and Maxillofacial Surgery, 2009
Journal of Oral and Maxillofacial Surgery, 2012
Journal of Oral and Maxillofacial Surgery, 2003
Cranial vault defects result from trauma, infection, tumor ablation or cerebral decompression pro... more Cranial vault defects result from trauma, infection, tumor ablation or cerebral decompression procedures. Cranial defects determine not only aesthetic but also functional alterations. The so-called syndrome of the trephined can be encountered in such patients. 1,2 The ...
Journal of Cranio-Maxillofacial Surgery, 2006
Tumor Biology, 2013
The present study was undertaken to examine the effects of cisplatin plus 4-hexylresorcinol (4-HR... more The present study was undertaken to examine the effects of cisplatin plus 4-hexylresorcinol (4-HR) combination therapy on oral mucosal melanoma (OMM) using cultured primary OMM cells in a tumour xenograft model. Cultured primary OMM cells were used for the MTT assay and DNA microarray. OMM cells were implanted into the submandibular glands of nude mice. The mice were then treated with cisplatin only or cisplatin plus 4-HR. Tumour size changes, survival rate and tumour metastasis were compared between the two groups by observation, micropositron emission tomography (PET) and histological examination. In the MTT assay, the cisplatin plus 4-HR group showed significantly higher inhibition of OMM cell growth compared to the other groups (p<0.05). DNA microarray results showed significant inhibition of matrix metalloproteinase (MMP)-2 gene expression upon 4-HR application. The necropsy and micro-PET results showed that the mice from the cisplatin-only group had more distant metastases than the mice from the cisplatin plus 4-HR combination group (p=0.002). MMP-2 expression was lower in the primary tumours in the cisplatin plus 4-HR combination group than in the cisplatin-only group (p<0.001). Overall survival was longer in mice from the cisplatin plus 4-HR combination group than in the cisplatin-only group (p=0.049). In conclusion, the combined effect of cisplatin and 4-HR resulted in fewer metastases and longer survival than cisplatin-only treatment in the OMM xenograft model.
Lasers in Medical Science
International Journal of Oral and Maxillofacial Surgery, 2009
fibula in reconstruction of mandibular defects. Methods: Twenty patients complaining of massive m... more fibula in reconstruction of mandibular defects. Methods: Twenty patients complaining of massive mandibular lesions were enrolled in this study. Group I (ten patients) were reconstructed by non-vascularised iliac crest and rib. Group II (ten patients) were reconstructed by vascularised fibula. Follow-up has extended up to the third postoperative year. Results and Conclusion: Microvascular fibula transfer proved to be a treatment of choice for reconstruction of long mandibular defects with simultaneous soft tissue covering and irradiated bone. Nonvascularised bone grafts are suitable for short defects, non-irradiated bone with a good vascular bed.
International Journal of Oral and Maxillofacial Surgery, 2007
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie, 2011
Osteonecrosis of the jaws is increasing worldwide in patients treated with bisphosphonates. A ret... more Osteonecrosis of the jaws is increasing worldwide in patients treated with bisphosphonates. A retrospective review of 52 patients who were treated during 2007-2010 for bisphosphonate related maxillofacial symptoms of the jaws was conducted. Patient characteristics and other factors that influenced the disease process were studied. Thirteen patients received bisphosphonates for the prevention or treatment of osteoporosis; 39 for preventing bone metastases from malignant tumors. Thirty-six patients were females (age range 32-87 years, median 64 years); 16 were males (age range 30-81 years, median 73.5 years). Bisphosphonate used was ibandronic acid in four cases, alendronate sodium in 14 cases, and zoledronic acid in 34 cases. Mean bisphosphonate treatment period was 22.44 months (95%CI 19.33-25.55). Thirty patients received intravenous, 22 received oral bisphosphonate. The average period until occurrence of maxillofacial symptoms was 6 months (range 0.5-24 months) in subjects with in...
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie, 2011
Lengthening the mandible by distraction osteogenesis (DO) is nowadays a well-recognized technique... more Lengthening the mandible by distraction osteogenesis (DO) is nowadays a well-recognized technique in maxillofacial surgery. This study compared two different distraction rates and evaluated histological and histomorphometrical properties of the distracted bone in an experimental ovine mandible model with the goal of elaborating a universally accepted distraction protocol. Tissue blocks of regenerated bone were harvested from twelve young adult sheep. DO was performed on the mandibular midline after five days of latency period. The sheep were divided into two groups. The first group underwent activation of 0.8 mm÷day during 12 days resulting in 9.6 mm of new bone while the second group followed a geometric rate pattern of 0.2 mm - three days, 0.4 mm - three days, 0.8 mm - three days and 1.6 mm - three days resulting in 9 mm of new bone. The regenerated bone was histologically and histomorphometrically analyzed after 30, 45 and 60 days of consolidation. The relative osteoid volume (OV...
Journal of Cranio-Maxillofacial Surgery, 2008
Objectives: To evaluate in preclinical models (in vitro and in animal model) all requests of Good... more Objectives: To evaluate in preclinical models (in vitro and in animal model) all requests of Good Manufacturing Practice's rules before the clinical application of mesenchymal stem cells (MSC), in order to elaborate the protocols for using human autologous MSC in oral regenerative and maxillofacial medicine. Methods: MSC lies in bone marrow an other tissues; they are autologous cells that have not immunological reaction and are expandable in vitro. MSC are taken from bone marrow sample from healthy donor. After medium procedures, an omogeneous SMC colony is reached and its osteoblast differenziating capacity was tested with or without bone morphogenetic protein-7 (BMP-7). Four medium were used (standard medium, growth medium, osteogenic medium, osteogenic medium with BMP-7). Results: The expression of osteogenic genes are analized by polimerase chain reaction (PCR): alkaline phosphatase (ALP), osteopontin (SPP1), osteocalcin (BGLAP). In the osteogenic medium with BMP-7, it was observed an increase of ALP, involved in the early phase of the osteogenetic differenziation, as well as an increase of SPP1 and BGLAP, involved in the late phase of osteogenetic differenziation and mineralization. Conclusions: Results suggest that BMP-7 amplifies the osteogenic differenziation process. These results are present in the quality, efficency and safety protocol: "maxillo-facial surgery bone defects by using autologous stem cells expansed in vitro.
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie, 2013
The objectives of this study were to test the biocompatibility and to evaluate the osseointegrati... more The objectives of this study were to test the biocompatibility and to evaluate the osseointegration of Titanium-Aluminum-Niobium (Ti6Al7Nb) alloy used in the manufacturing of personalized implants with selective laser melting (SLM) technology and to compare the growth viability of osteoblastic-like cells on different Ti6Al7Nb alloy samples (plain, coated with hydroxyapatite or SiO2-TiO2) implanted into the cranial bone of Wistar rats. In terms of biocompatibility, the cone-beam computer-tomography head scans taken at the moment of sacrifice of each group (one, two and three months) showed no implant displacement, no osteolysis and no liquid collection around the implants. At one month, around all types of implants new bone formation was noticed, although around the plain Ti6Al7Nb implant a large amount of powder debris was present. Still, no inflammatory reaction was seen. At two months, the distance between the implants and the calvarial bone margins diminished. A thin layer of fib...
Particulate Science and Technology
International Journal of Oral and Maxillofacial Surgery, 2005
Revista medico-chirurgicală̆ a Societă̆ţ̜ii de Medici ş̧i Naturaliş̧ti din Iaş̧i
Life quality has a major importance in the actual social context. The evaluation af the pain sens... more Life quality has a major importance in the actual social context. The evaluation af the pain sensation as a stress major factor is difficult regarding the variety of the methods, their relevance and their compliance. We've made an evaluation concerning the trigeminal essential neuralgia pain using different tipe of parameters. The results obtained proved a scientific and clinical value of the method that we've initiated and applied in these research.
Revista medico-chirurgicală̆ a Societă̆ţ̜ii de Medici ş̧i Naturaliş̧ti din Iaş̧i
Arterio-venous vascular malformations (AVM) rarely occur in craniofacial bones. The most frequent... more Arterio-venous vascular malformations (AVM) rarely occur in craniofacial bones. The most frequent location has been reported in the mandible. The first clinical evidence of the lesions can be a fatal hemorrhage, occurring spontaneously or subsequent to dental therapy. Lack of knowledge about these lesions among specialists dealing with the oral and maxillofacial regions can lead to fatal complications. The study presents the successful multidisciplinary management of two cases of mandibular AVM using embolization, sclerotherapy and bone reconstruction, a major challenge owing to the substantial risk of morbidity, recurrence by recanalization and revascularization, neurologic deficits and mortality. There were no complications over a follow-up period of 12 years.
Revista medico-chirurgicală̆ a Societă̆ţ̜ii de Medici ş̧i Naturaliş̧ti din Iaş̧i
This case report highlights the possibility of a non extraction orthodontic treatment, surgically... more This case report highlights the possibility of a non extraction orthodontic treatment, surgically assisted, in a Class II Division 1 malocclusions in order to reduce maxillary protrusion prior to the surgical advancement of the mandible for the correction of the skeletal deficiency. The combined orthodontic-surgical approach optimizes the profile while allowing the settling of a Angle Class I occlusion at the end of the entire treatment sequence.
Revista medico-chirurgicală̆ a Societă̆ţ̜ii de Medici ş̧i Naturaliş̧ti din Iaş̧i
The interdisciplinary, complex therapeutic protocol of the cleft lip and palate patients, applied... more The interdisciplinary, complex therapeutic protocol of the cleft lip and palate patients, applied in the Clinic of Cranio-Maxillo-Facial Surgery of "I. Haţieganu" University of Medicine and Pharmacy Cluj-Napoca, involves the morphologic reconstruction as well as the functional rehabilitation. Functional rehabilitation is the aspect, which gives the esthetics, social and familial integration of the patient, offering good quality of life. In the current study, a new method and concept of improving the phonetic function in the primary and secondary surgical steps, with the effect on muscle and bone, is presented. The new surgical techniques used comprise of the surgery of the levator veli palatini using the method designed by Sader, and bone distraction, during the same surgical procedure. The assessment of the phonetic results was performed using the NARSOM test. Following up the results of the techniques mentioned above, we consider that they improve extremely well the morp...
International Journal of Oral and Maxillofacial Surgery, 2009
Journal of Oral and Maxillofacial Surgery, 2012
Journal of Oral and Maxillofacial Surgery, 2003
Cranial vault defects result from trauma, infection, tumor ablation or cerebral decompression pro... more Cranial vault defects result from trauma, infection, tumor ablation or cerebral decompression procedures. Cranial defects determine not only aesthetic but also functional alterations. The so-called syndrome of the trephined can be encountered in such patients. 1,2 The ...
Journal of Cranio-Maxillofacial Surgery, 2006
Tumor Biology, 2013
The present study was undertaken to examine the effects of cisplatin plus 4-hexylresorcinol (4-HR... more The present study was undertaken to examine the effects of cisplatin plus 4-hexylresorcinol (4-HR) combination therapy on oral mucosal melanoma (OMM) using cultured primary OMM cells in a tumour xenograft model. Cultured primary OMM cells were used for the MTT assay and DNA microarray. OMM cells were implanted into the submandibular glands of nude mice. The mice were then treated with cisplatin only or cisplatin plus 4-HR. Tumour size changes, survival rate and tumour metastasis were compared between the two groups by observation, micropositron emission tomography (PET) and histological examination. In the MTT assay, the cisplatin plus 4-HR group showed significantly higher inhibition of OMM cell growth compared to the other groups (p<0.05). DNA microarray results showed significant inhibition of matrix metalloproteinase (MMP)-2 gene expression upon 4-HR application. The necropsy and micro-PET results showed that the mice from the cisplatin-only group had more distant metastases than the mice from the cisplatin plus 4-HR combination group (p=0.002). MMP-2 expression was lower in the primary tumours in the cisplatin plus 4-HR combination group than in the cisplatin-only group (p<0.001). Overall survival was longer in mice from the cisplatin plus 4-HR combination group than in the cisplatin-only group (p=0.049). In conclusion, the combined effect of cisplatin and 4-HR resulted in fewer metastases and longer survival than cisplatin-only treatment in the OMM xenograft model.