H. Sevestre - Academia.edu (original) (raw)
Papers by H. Sevestre
La Revue de Médecine Interne, 2007
Résultats.-Cette étude a porté sur 29 patients (24 femmes, cinq hommes, âge moy = 68,5 ans, CRP m... more Résultats.-Cette étude a porté sur 29 patients (24 femmes, cinq hommes, âge moy = 68,5 ans, CRP moy = 125 mg/l). Un signe clinique d'ischémie des membres supérieurs et des membres inférieurs était retrouvé dans 17 et 20 % des cas. L'ED des vaisseaux cervicaux était considérée comme normale chez 15 / 29 patients alors que huit patients (27,5 %) étaient porteurs de sténose artérielle de 20 à 60 % : carotide interne (n = 8), carotide primitive (n = 2), sous-clavière (n = 1). Il n'était visualisé aucune ectasie ni halo mais un épaississement pariétal de 1,2 à 1,5 mm était noté chez trois patients (10,3 %) : au niveau sous-clavier (n = 1), de la carotide primitive (n = 2), de la carotide interne (n = 3). Les artères des membres inférieurs étaient normales dans 76 % des cas. Seuls trois patients (10,3 %) étaient porteurs d'une sténose artérielle (40 à 60 %) : artère fémorale superficielle (n = 2), fémorale profonde (n = 2), poplitée (n = 1). Un halo hypoéchogène bilatéral de 1,6 à 3 mm était retrouvé dans un cas au niveau du tiers supérieur de l'artère fémorale superficielle. Il n'était pas retrouvé de corrélation entre la présence des signes cliniques ischémiques et les anomalies ED. Conclusion.-Cette étude montre qu'au stade initial de MH, les sténoses artérielles les plus fréquentes sont situées au niveau des carotides internes (27,5 %), associées à des plaques d'athérome d'allure banale. Dans la grande majorité des cas, les artères axillo-sous-clavières, décrites pourtant comme une cible préférentielle de l'inflammation au cours de la MH sont normales en ED. Dans notre expérience, la présence d'un halo hypoéchogène périvasculaire est rare (un seul cas). Si elle semble peu fréquente au stade initial, l'inflammation des artères de gros calibre pourrait davantage survenir comme complication évolutive d'une MH.
La Revue de Médecine Interne, 2009
Progrès en Urologie, 2010
ABSTRACT Prospective evaluation of bladder tumor targeting by Hexvix(®) fluorescence. From Septem... more ABSTRACT Prospective evaluation of bladder tumor targeting by Hexvix(®) fluorescence. From September 2008 to September 2009, 28 patients have been evaluated using Hexvix(®) technique (blue light) for typically papillary bladder tumor or suspected bladder tumor. Clinical and pathological data have been collected and patients have been classified using EORTC score. From the 28 patients, 14 have been identified with bladder cancer (31 tumors) and 90 samples have shown positive fluorescence in blue light. Compared to white light, four additional tumors have been diagnosed by Hexvix(®) for three patients (p=NS): two cis, one LMP, one pTa low grade. The false positive rate for Hexvix(®) was 65.5 versus 58.5% for white light. EORTC score for bladder cancer was associated with better bladder cancer targeting by Hexvix(®) (p<0.05). In our experience, Hexvix(®) targeting was associated with better diagnosis for bladder cancer. Thirteen percent of new positive samples have been found for 10.7% of patients. In our study false positive rate is a critical point (65.5%). EORTC score for recurrence and progression might allow to select patients to be targeted by Hexvix(®).
Gynécologie Obstétrique & Fertilité, 2006
ABSTRACT
Gynécologie Obstétrique & Fertilité, 2003
Morphologie : bulletin de l'Association des anatomistes, 2007
In order to enhance the knowledge of the internal thoracic arteries of pig, sheep, and man, allow... more In order to enhance the knowledge of the internal thoracic arteries of pig, sheep, and man, allowing to constitute a fundamental basis as for the current and later applications in the interventions in surgery of revascularization of the arteries of less than four millimetre of diameter, a biometric and histological comparative study of these arteries was carried out. Forty human corpses, 27 men and 13 women (mean age 75+/-6 years) were dissected, alike with three pigs respectively weighing 80 kg, 80 kg and 84 kg and four sheep weighing 70 kg each. The left and right internal thoracic arteries were harvested: exposure over the entire length and remote dissection before excision. At the same time their internal lengths and gauges were measured. The internal thoracic artery (ITA) of human has an average useful length of 18 cm and an average internal gauge close to 1.5 mm. ITA of the pig has an average length of 27 cm and an average internal gauge close to 2.8 mm. The ITA of the sheep h...
Annales de pathologie, 2000
We report the case of a 14-year-old girl with a testicular feminization syndrome. The inguinal cr... more We report the case of a 14-year-old girl with a testicular feminization syndrome. The inguinal cryptorchid testis contained plurifocal hamartomas ranging from 0.5 to 1 cm. They were composed of tubules lined by cylindrical Sertoli cells immunoreactive for alpha-inhibin and p30/32(MIC2). The stroma contained few Leydig cells. Ultrastructural study showed tubules with immature Sertoli cells. The testicular feminization syndrome is caused by mutations of the androgen receptor gene. Patients with male genotype 46, XY have a female morphotype with external sexual organs without ambiguity. They have neither uterus nor ovary but two cryptorchid testis in which sex-cord stromal tumors can develop. Their malignant transformation is rare but requires preventive bilateral orchidectomy.
Gastroentérologie clinique et biologique, 1994
Revue des Maladies Respiratoires, 2005
Surgical and Radiologic Anatomy, 2007
The biometry and the histology of coronary, radial, ulnar, epigastric and internal thoracic arter... more The biometry and the histology of coronary, radial, ulnar, epigastric and internal thoracic arteries were studied in order to investigate the cause of their occlusions in coronary bypass grafts and to improve the results of these bypass grafts. These various arteries were removed from 40 anatomical specimens (27 males and 13 females). We found a correlation between the internal calibers of the ulnar and coronary arteries in males. Intimal changes and the presence of atheromatous plaque were observed in coronary, radial and ulnar arteries, but never in the internal thoracic artery. Like coronary arteries and their branches, radial, ulnar and epigastric arteries are muscular arteries and ageing results in thickening of the intima, which becomes Wbrotic with migration of myocytes from the media and duplication of the internal elastic lamina. The media becomes Wbrous, hypertrophic or atrophic. In contrast, the internal thoracic artery is an elastic artery, like the aorta. Ageing is characterized by loss, over a variable extent, of one or several elastic laminae of the media and more marked intimal thickening. Although anatomically, the caliber of radial, ulnar, and epigastric arteries remains adapted to that of coronary arteries, the long-term patency of radial, ulnar and epigastric arteries used as grafts is related to their histological characteristics.
Respiratory Medicine, 1998
Journal of Medical Microbiology, 2004
Three specimens from 111 asymptomatic male partners of infertile couples attending the Department... more Three specimens from 111 asymptomatic male partners of infertile couples attending the Department of Urology in Amiens, France, were examined by the PCR COBAS AMPLICOR test (Roche Molecular Diagnostics) for the presence of Chlamydia trachomatis. The specimens analysed were: first void urine (FVU), urine obtained after prostatic massage (UPM) and semen specimens. Serum from each patient was also obtained and analysed for the presence of IgG and IgA chlamydial antibodies by in-house microimmunofluorescence (MIF) and pELISA. C. trachomatis was detected by PCR in 5. 4 % of FVU samples, 2. 7 % of semen specimens and in 0. 9 % of UPM samples. Two treatments for processing the samples (storage at À70 8C and heating to 95 8C) were routinely used before initial testing to reduce the effects of inhibitors of PCR. Despite these precautions, the PCR method revealed the presence of inhibitors in 7. 3 % of semen specimens and 3. 6 % of FVU samples. C. trachomatis was detected by PCR COBAS AMPLICOR in seven of 111 patients (6. 3 %) and by serology in five of 111 patients (4. 5 %). The detection of C. trachomatis in FVU, UPM and semen specimens can serve as a marker for the presence of this organism in the genital tract, and can be used as a reliable way of detecting asymptomatic carriers of infection.
Journal of Clinical Virology, 2008
Background: Epidemiological data on human papillomavirus (HPV) are needed to estimate potential c... more Background: Epidemiological data on human papillomavirus (HPV) are needed to estimate potential changes in type distribution induced by recent HPV vaccination strategies. Objectives and study design: The epidemiological distribution of HPV in 669 cervical specimens from French women with and without cytological abnormalities was evaluated using type-specific PCR or sequencing. The results were compared with those obtained using the Digene ® high-risk Hybrid Capture 2 (HR-HC2) assay. Results: The overall prevalence of HPV was high (45.3%) in our study population. 285 of the 291 HPV-positive samples were typed. The distribution frequency concerned 34 different genotypes, with HPV16 being the most prevalent (32.6%). Other genotypes present were HPV31 (7.4%), HPV18, HPV 52 (both 6.0%), HPV6 (5.3%) and HPV66 (4.2%). The respective frequencies of all other genotypes were below 4%. The agreement with HR-HC2 was 78.8%. The distribution frequency data were also analyzed relatively to cytological and histological results. Our method enables the diagnosis of HPV infections with the additional advantage of genotyping. Conclusion: HPV infections in the area of France studied here involve numerous HPV types, but the high cumulative prevalences of types 16, 18, 6 and 11 (44.6% in total) would suggest a major impact of vaccination on these genotypes.
Gastroentérologie Clinique et Biologique, 2008
Disponible sur Internet le 18 mars 2008 Résumé Nous rapportons une observation rare de polype fib... more Disponible sur Internet le 18 mars 2008 Résumé Nous rapportons une observation rare de polype fibroïde inflammatoire de l'appendice. Une femme de 33 ans consultait pour des douleurs abdominales accompagnées d'un fébricule et d'une défense de l'hypochondre droit à la palpation. Le scanner abdominal révélait la présence d'une tumeur de 8 cm de l'appendice. Une appendicectomie sous coelioscopie était réalisée. À l'examen histologique, la tumeur était composée de cellules fusiformes et étoilées siégeant au sein d'un stroma fibromyxoïde richement vascularisé et comportant un infiltrat inflammatoire notable riche en polynucléaires éosinophiles. À l'étude immunohistochimique les cellules tumorales exprimaient la vimentine, la fascine et focalement le CD34 et le CD35. Elles n'exprimaient pas l'actine, la desmine, le CD21, le CD23, le CD117 et la protéine S100. Le polype fibroïde inflammatoire est une tumeur mésenchymateuse bénigne du tractus gastro-intestinal, rarement rapporté au niveau de l'appendice. Il partage des caractéristiques morphologiques communes avec la tumeur myofibroblastique inflammatoire, mais correspond à une entité distincte. L'histogenèse de cette tumeur reste mal connue, mais l'expression de la fascine et du CD35 par les cellules tumorales suggère qu'elle pourrait dériver des cellules dendritiques.
Annales de Pathologie, 2006
Annales de Pathologie, 2006
American Journal of Physiology-Cell Physiology, 2009
Because transient receptor potential (TRP) channels have been implicated in tumor progression, we... more Because transient receptor potential (TRP) channels have been implicated in tumor progression, we have investigated the potential role of TRPM7 channel in breast cancer cell proliferation. Under whole cell patch clamp, a Mg2+-inhibited cationic (MIC) current was observed in MCF-7 cells. This current was characterized by an inward current and a strong outward rectifying current that were both inhibited in a concentration-dependent manner by the presence of intracellular Mg2+or Mg2+-ATP. The inward current was reduced by La3+, and the outward current was sensitive to 2-aminoethoxydiphenyl borate (2-APB), spermine, La3+, and flufenamic acid. Importantly, a similar MIC current was also recorded in the primary culture of human breast cancerous epithelial cells (hBCE). Moreover, TRPM7 transcripts were found in both hBCE and MCF-7 cells. In MCF-7 cells, the MIC current was inhibited by TRPM7 small interfering RNA. Interestingly, we found that cell proliferation and intracellular Ca2+concen...
Advances in hematology, 2009
Background. To better describe the clinical, biological, and the outcome of non-Hodgkin's lym... more Background. To better describe the clinical, biological, and the outcome of non-Hodgkin's lymphoma (NHL) with, at the initial presentation, bone marrow fibrosis (MF). Patients and Methods. From January 2001 to January 2007, 16 eligible patients with NHL and MF were retrieved from the Pathology Department of the University hospital of Amiens. Median age of patients was 62 years (range 16-74) with a sex ratio male/female of 3. Results. MF is associated with all types of lymphoma predominantly with B-cell phenotype and it seems to be more associated with low-grade NHL. B-symptoms are more frequent at diagnosis and more patients presented with an elevated LDH level. JAK-2 was negative in the 10 patients analysed. Two patients presented with features of primary MF with no evidence of lymphoma. Overall response rate was 94% after the first line of therapy with regression or improvement of MF. Relapse occurred in 8 patients (47%) with recurrence of MF in all of them. After a median fol...
Circulation, 2008
Background— Chronic renal failure (CRF) is associated with cardiac dysfunction and increased aort... more Background— Chronic renal failure (CRF) is associated with cardiac dysfunction and increased aortic stiffness. The mechanisms involved are not clearly understood. We examined changes over time in cardiac and aortic function in a murine CRF model. Methods and Results— Eight-week-old mice were randomly assigned to 1 of 4 groups: wild-type non-CRF, wild-type CRF, apolipoprotein E knockout non-CRF, and apolipoprotein E knockout CRF. Echocardiography was performed and blood samples were taken at baseline and after 6 and 10 weeks of CRF. Vascular reactivity and adhesion molecule expression were studied after 6 and 10 weeks of CRF. Left ventricular hypertrophy, altered left ventricular relaxation, and increased aortic stiffness were observed after 6 weeks of CRF and persisted after 10 weeks. The 4 groups of mice did not significantly differ in terms of arterial blood pressure and aortic structure. The degree of vascular calcification and serum total cholesterol concentration were higher in...
Acta Ophthalmologica, 2012
ABSTRACT Purpose Optic pathway gliomas are rare tumors accounting for 3-5% of brain tumors in chi... more ABSTRACT Purpose Optic pathway gliomas are rare tumors accounting for 3-5% of brain tumors in children. Our case aims to understand surgical options. Methods We report the case of a 4-year-old child with a single optic pathway glioma revealed by a non esthetical proptosis and blindness. Association with NF1 was not found. The MRI showed a tortuous enlargement of the left optic nerve, the glioma extended into the optic canal with a scleral invasion. Considering those results, a treatment consisting of a double team surgery, ophthalmologists and neurosurgeons was performed with enucleation and complete section of the nerve at its optic chiasm root. Results Histology confirmed the diagnosis of glioma, as a pilocytic astrocytoma, with safety margins. Cytogenetic analysis showed an unspecific chromosome 8 deletion. Chemotherapy was avoided because of systemic toxicity and tumor localization. Conclusion Association with NF1 is classical and the incidence of optic nerve glioma is estimated at 15%. In young children the glioma can be the first manifestation of NF1 and the disease should be kept in mind. Cytogenetic mutation must be searched with surgical sample in culture. In children, surgical treatment can be preferred to chemotherapy when excision of the total tumor removal can be performed.
La Revue de Médecine Interne, 2007
Résultats.-Cette étude a porté sur 29 patients (24 femmes, cinq hommes, âge moy = 68,5 ans, CRP m... more Résultats.-Cette étude a porté sur 29 patients (24 femmes, cinq hommes, âge moy = 68,5 ans, CRP moy = 125 mg/l). Un signe clinique d'ischémie des membres supérieurs et des membres inférieurs était retrouvé dans 17 et 20 % des cas. L'ED des vaisseaux cervicaux était considérée comme normale chez 15 / 29 patients alors que huit patients (27,5 %) étaient porteurs de sténose artérielle de 20 à 60 % : carotide interne (n = 8), carotide primitive (n = 2), sous-clavière (n = 1). Il n'était visualisé aucune ectasie ni halo mais un épaississement pariétal de 1,2 à 1,5 mm était noté chez trois patients (10,3 %) : au niveau sous-clavier (n = 1), de la carotide primitive (n = 2), de la carotide interne (n = 3). Les artères des membres inférieurs étaient normales dans 76 % des cas. Seuls trois patients (10,3 %) étaient porteurs d'une sténose artérielle (40 à 60 %) : artère fémorale superficielle (n = 2), fémorale profonde (n = 2), poplitée (n = 1). Un halo hypoéchogène bilatéral de 1,6 à 3 mm était retrouvé dans un cas au niveau du tiers supérieur de l'artère fémorale superficielle. Il n'était pas retrouvé de corrélation entre la présence des signes cliniques ischémiques et les anomalies ED. Conclusion.-Cette étude montre qu'au stade initial de MH, les sténoses artérielles les plus fréquentes sont situées au niveau des carotides internes (27,5 %), associées à des plaques d'athérome d'allure banale. Dans la grande majorité des cas, les artères axillo-sous-clavières, décrites pourtant comme une cible préférentielle de l'inflammation au cours de la MH sont normales en ED. Dans notre expérience, la présence d'un halo hypoéchogène périvasculaire est rare (un seul cas). Si elle semble peu fréquente au stade initial, l'inflammation des artères de gros calibre pourrait davantage survenir comme complication évolutive d'une MH.
La Revue de Médecine Interne, 2009
Progrès en Urologie, 2010
ABSTRACT Prospective evaluation of bladder tumor targeting by Hexvix(®) fluorescence. From Septem... more ABSTRACT Prospective evaluation of bladder tumor targeting by Hexvix(®) fluorescence. From September 2008 to September 2009, 28 patients have been evaluated using Hexvix(®) technique (blue light) for typically papillary bladder tumor or suspected bladder tumor. Clinical and pathological data have been collected and patients have been classified using EORTC score. From the 28 patients, 14 have been identified with bladder cancer (31 tumors) and 90 samples have shown positive fluorescence in blue light. Compared to white light, four additional tumors have been diagnosed by Hexvix(®) for three patients (p=NS): two cis, one LMP, one pTa low grade. The false positive rate for Hexvix(®) was 65.5 versus 58.5% for white light. EORTC score for bladder cancer was associated with better bladder cancer targeting by Hexvix(®) (p<0.05). In our experience, Hexvix(®) targeting was associated with better diagnosis for bladder cancer. Thirteen percent of new positive samples have been found for 10.7% of patients. In our study false positive rate is a critical point (65.5%). EORTC score for recurrence and progression might allow to select patients to be targeted by Hexvix(®).
Gynécologie Obstétrique & Fertilité, 2006
ABSTRACT
Gynécologie Obstétrique & Fertilité, 2003
Morphologie : bulletin de l'Association des anatomistes, 2007
In order to enhance the knowledge of the internal thoracic arteries of pig, sheep, and man, allow... more In order to enhance the knowledge of the internal thoracic arteries of pig, sheep, and man, allowing to constitute a fundamental basis as for the current and later applications in the interventions in surgery of revascularization of the arteries of less than four millimetre of diameter, a biometric and histological comparative study of these arteries was carried out. Forty human corpses, 27 men and 13 women (mean age 75+/-6 years) were dissected, alike with three pigs respectively weighing 80 kg, 80 kg and 84 kg and four sheep weighing 70 kg each. The left and right internal thoracic arteries were harvested: exposure over the entire length and remote dissection before excision. At the same time their internal lengths and gauges were measured. The internal thoracic artery (ITA) of human has an average useful length of 18 cm and an average internal gauge close to 1.5 mm. ITA of the pig has an average length of 27 cm and an average internal gauge close to 2.8 mm. The ITA of the sheep h...
Annales de pathologie, 2000
We report the case of a 14-year-old girl with a testicular feminization syndrome. The inguinal cr... more We report the case of a 14-year-old girl with a testicular feminization syndrome. The inguinal cryptorchid testis contained plurifocal hamartomas ranging from 0.5 to 1 cm. They were composed of tubules lined by cylindrical Sertoli cells immunoreactive for alpha-inhibin and p30/32(MIC2). The stroma contained few Leydig cells. Ultrastructural study showed tubules with immature Sertoli cells. The testicular feminization syndrome is caused by mutations of the androgen receptor gene. Patients with male genotype 46, XY have a female morphotype with external sexual organs without ambiguity. They have neither uterus nor ovary but two cryptorchid testis in which sex-cord stromal tumors can develop. Their malignant transformation is rare but requires preventive bilateral orchidectomy.
Gastroentérologie clinique et biologique, 1994
Revue des Maladies Respiratoires, 2005
Surgical and Radiologic Anatomy, 2007
The biometry and the histology of coronary, radial, ulnar, epigastric and internal thoracic arter... more The biometry and the histology of coronary, radial, ulnar, epigastric and internal thoracic arteries were studied in order to investigate the cause of their occlusions in coronary bypass grafts and to improve the results of these bypass grafts. These various arteries were removed from 40 anatomical specimens (27 males and 13 females). We found a correlation between the internal calibers of the ulnar and coronary arteries in males. Intimal changes and the presence of atheromatous plaque were observed in coronary, radial and ulnar arteries, but never in the internal thoracic artery. Like coronary arteries and their branches, radial, ulnar and epigastric arteries are muscular arteries and ageing results in thickening of the intima, which becomes Wbrotic with migration of myocytes from the media and duplication of the internal elastic lamina. The media becomes Wbrous, hypertrophic or atrophic. In contrast, the internal thoracic artery is an elastic artery, like the aorta. Ageing is characterized by loss, over a variable extent, of one or several elastic laminae of the media and more marked intimal thickening. Although anatomically, the caliber of radial, ulnar, and epigastric arteries remains adapted to that of coronary arteries, the long-term patency of radial, ulnar and epigastric arteries used as grafts is related to their histological characteristics.
Respiratory Medicine, 1998
Journal of Medical Microbiology, 2004
Three specimens from 111 asymptomatic male partners of infertile couples attending the Department... more Three specimens from 111 asymptomatic male partners of infertile couples attending the Department of Urology in Amiens, France, were examined by the PCR COBAS AMPLICOR test (Roche Molecular Diagnostics) for the presence of Chlamydia trachomatis. The specimens analysed were: first void urine (FVU), urine obtained after prostatic massage (UPM) and semen specimens. Serum from each patient was also obtained and analysed for the presence of IgG and IgA chlamydial antibodies by in-house microimmunofluorescence (MIF) and pELISA. C. trachomatis was detected by PCR in 5. 4 % of FVU samples, 2. 7 % of semen specimens and in 0. 9 % of UPM samples. Two treatments for processing the samples (storage at À70 8C and heating to 95 8C) were routinely used before initial testing to reduce the effects of inhibitors of PCR. Despite these precautions, the PCR method revealed the presence of inhibitors in 7. 3 % of semen specimens and 3. 6 % of FVU samples. C. trachomatis was detected by PCR COBAS AMPLICOR in seven of 111 patients (6. 3 %) and by serology in five of 111 patients (4. 5 %). The detection of C. trachomatis in FVU, UPM and semen specimens can serve as a marker for the presence of this organism in the genital tract, and can be used as a reliable way of detecting asymptomatic carriers of infection.
Journal of Clinical Virology, 2008
Background: Epidemiological data on human papillomavirus (HPV) are needed to estimate potential c... more Background: Epidemiological data on human papillomavirus (HPV) are needed to estimate potential changes in type distribution induced by recent HPV vaccination strategies. Objectives and study design: The epidemiological distribution of HPV in 669 cervical specimens from French women with and without cytological abnormalities was evaluated using type-specific PCR or sequencing. The results were compared with those obtained using the Digene ® high-risk Hybrid Capture 2 (HR-HC2) assay. Results: The overall prevalence of HPV was high (45.3%) in our study population. 285 of the 291 HPV-positive samples were typed. The distribution frequency concerned 34 different genotypes, with HPV16 being the most prevalent (32.6%). Other genotypes present were HPV31 (7.4%), HPV18, HPV 52 (both 6.0%), HPV6 (5.3%) and HPV66 (4.2%). The respective frequencies of all other genotypes were below 4%. The agreement with HR-HC2 was 78.8%. The distribution frequency data were also analyzed relatively to cytological and histological results. Our method enables the diagnosis of HPV infections with the additional advantage of genotyping. Conclusion: HPV infections in the area of France studied here involve numerous HPV types, but the high cumulative prevalences of types 16, 18, 6 and 11 (44.6% in total) would suggest a major impact of vaccination on these genotypes.
Gastroentérologie Clinique et Biologique, 2008
Disponible sur Internet le 18 mars 2008 Résumé Nous rapportons une observation rare de polype fib... more Disponible sur Internet le 18 mars 2008 Résumé Nous rapportons une observation rare de polype fibroïde inflammatoire de l'appendice. Une femme de 33 ans consultait pour des douleurs abdominales accompagnées d'un fébricule et d'une défense de l'hypochondre droit à la palpation. Le scanner abdominal révélait la présence d'une tumeur de 8 cm de l'appendice. Une appendicectomie sous coelioscopie était réalisée. À l'examen histologique, la tumeur était composée de cellules fusiformes et étoilées siégeant au sein d'un stroma fibromyxoïde richement vascularisé et comportant un infiltrat inflammatoire notable riche en polynucléaires éosinophiles. À l'étude immunohistochimique les cellules tumorales exprimaient la vimentine, la fascine et focalement le CD34 et le CD35. Elles n'exprimaient pas l'actine, la desmine, le CD21, le CD23, le CD117 et la protéine S100. Le polype fibroïde inflammatoire est une tumeur mésenchymateuse bénigne du tractus gastro-intestinal, rarement rapporté au niveau de l'appendice. Il partage des caractéristiques morphologiques communes avec la tumeur myofibroblastique inflammatoire, mais correspond à une entité distincte. L'histogenèse de cette tumeur reste mal connue, mais l'expression de la fascine et du CD35 par les cellules tumorales suggère qu'elle pourrait dériver des cellules dendritiques.
Annales de Pathologie, 2006
Annales de Pathologie, 2006
American Journal of Physiology-Cell Physiology, 2009
Because transient receptor potential (TRP) channels have been implicated in tumor progression, we... more Because transient receptor potential (TRP) channels have been implicated in tumor progression, we have investigated the potential role of TRPM7 channel in breast cancer cell proliferation. Under whole cell patch clamp, a Mg2+-inhibited cationic (MIC) current was observed in MCF-7 cells. This current was characterized by an inward current and a strong outward rectifying current that were both inhibited in a concentration-dependent manner by the presence of intracellular Mg2+or Mg2+-ATP. The inward current was reduced by La3+, and the outward current was sensitive to 2-aminoethoxydiphenyl borate (2-APB), spermine, La3+, and flufenamic acid. Importantly, a similar MIC current was also recorded in the primary culture of human breast cancerous epithelial cells (hBCE). Moreover, TRPM7 transcripts were found in both hBCE and MCF-7 cells. In MCF-7 cells, the MIC current was inhibited by TRPM7 small interfering RNA. Interestingly, we found that cell proliferation and intracellular Ca2+concen...
Advances in hematology, 2009
Background. To better describe the clinical, biological, and the outcome of non-Hodgkin's lym... more Background. To better describe the clinical, biological, and the outcome of non-Hodgkin's lymphoma (NHL) with, at the initial presentation, bone marrow fibrosis (MF). Patients and Methods. From January 2001 to January 2007, 16 eligible patients with NHL and MF were retrieved from the Pathology Department of the University hospital of Amiens. Median age of patients was 62 years (range 16-74) with a sex ratio male/female of 3. Results. MF is associated with all types of lymphoma predominantly with B-cell phenotype and it seems to be more associated with low-grade NHL. B-symptoms are more frequent at diagnosis and more patients presented with an elevated LDH level. JAK-2 was negative in the 10 patients analysed. Two patients presented with features of primary MF with no evidence of lymphoma. Overall response rate was 94% after the first line of therapy with regression or improvement of MF. Relapse occurred in 8 patients (47%) with recurrence of MF in all of them. After a median fol...
Circulation, 2008
Background— Chronic renal failure (CRF) is associated with cardiac dysfunction and increased aort... more Background— Chronic renal failure (CRF) is associated with cardiac dysfunction and increased aortic stiffness. The mechanisms involved are not clearly understood. We examined changes over time in cardiac and aortic function in a murine CRF model. Methods and Results— Eight-week-old mice were randomly assigned to 1 of 4 groups: wild-type non-CRF, wild-type CRF, apolipoprotein E knockout non-CRF, and apolipoprotein E knockout CRF. Echocardiography was performed and blood samples were taken at baseline and after 6 and 10 weeks of CRF. Vascular reactivity and adhesion molecule expression were studied after 6 and 10 weeks of CRF. Left ventricular hypertrophy, altered left ventricular relaxation, and increased aortic stiffness were observed after 6 weeks of CRF and persisted after 10 weeks. The 4 groups of mice did not significantly differ in terms of arterial blood pressure and aortic structure. The degree of vascular calcification and serum total cholesterol concentration were higher in...
Acta Ophthalmologica, 2012
ABSTRACT Purpose Optic pathway gliomas are rare tumors accounting for 3-5% of brain tumors in chi... more ABSTRACT Purpose Optic pathway gliomas are rare tumors accounting for 3-5% of brain tumors in children. Our case aims to understand surgical options. Methods We report the case of a 4-year-old child with a single optic pathway glioma revealed by a non esthetical proptosis and blindness. Association with NF1 was not found. The MRI showed a tortuous enlargement of the left optic nerve, the glioma extended into the optic canal with a scleral invasion. Considering those results, a treatment consisting of a double team surgery, ophthalmologists and neurosurgeons was performed with enucleation and complete section of the nerve at its optic chiasm root. Results Histology confirmed the diagnosis of glioma, as a pilocytic astrocytoma, with safety margins. Cytogenetic analysis showed an unspecific chromosome 8 deletion. Chemotherapy was avoided because of systemic toxicity and tumor localization. Conclusion Association with NF1 is classical and the incidence of optic nerve glioma is estimated at 15%. In young children the glioma can be the first manifestation of NF1 and the disease should be kept in mind. Cytogenetic mutation must be searched with surgical sample in culture. In children, surgical treatment can be preferred to chemotherapy when excision of the total tumor removal can be performed.