Hadir Ahmed - Academia.edu (original) (raw)
Uploads
Papers by Hadir Ahmed
Minia Journal of Medical Research, 2019
Mohamed et al., 150 Platelet to Lymphocyte ratio and Neutrophil to Lymphocyte ratio as new diagno... more Mohamed et al., 150 Platelet to Lymphocyte ratio and Neutrophil to Lymphocyte ratio as new diagnostic markers for detection of Early-onset
Background: Carbapenem resistant extended spectrum β-lactamase (ESBL) producing Klebsiella pneumo... more Background: Carbapenem resistant extended spectrum β-lactamase (ESBL) producing Klebsiella pneumoniae (K. pneumoniae) is increasing worldwide. Carbapenem resistance (CR) has been attributed not only to production of carbapenemases but also to permeability barriers due to outer membrane proteins (OmpK35 and OmpK36) disruption. Objective: Phenotypic detection of CR among ESBL producing K. pneumoniae isolates, followed by the evaluation of the role of ompK35 and ompK36 gene expression among carbapenem resistant K. pneumoniae (CR-KP) isolates. Methods: 100 ESBL producing K. pneumoniae isolates were included in this study. Minimum inhibitory concentration (MIC) of imipenem was performed for all isolates by broth microdilution method. For CR-KP isolates, phenotypic detection of K. pneumoniae carbapenemase (KPC), metallo-β-lactamase (MBL) and AmpC enzymes was performed followed by Realtime qRT-PCR to detect and quantify ompK35 and ompK36 gene expression. Results: 42% of our isolates were c...
Minia Journal of Medical Research, 2019
Mohamed et al., 150 Platelet to Lymphocyte ratio and Neutrophil to Lymphocyte ratio as new diagno... more Mohamed et al., 150 Platelet to Lymphocyte ratio and Neutrophil to Lymphocyte ratio as new diagnostic markers for detection of Early-onset
Background: Carbapenem resistant extended spectrum β-lactamase (ESBL) producing Klebsiella pneumo... more Background: Carbapenem resistant extended spectrum β-lactamase (ESBL) producing Klebsiella pneumoniae (K. pneumoniae) is increasing worldwide. Carbapenem resistance (CR) has been attributed not only to production of carbapenemases but also to permeability barriers due to outer membrane proteins (OmpK35 and OmpK36) disruption. Objective: Phenotypic detection of CR among ESBL producing K. pneumoniae isolates, followed by the evaluation of the role of ompK35 and ompK36 gene expression among carbapenem resistant K. pneumoniae (CR-KP) isolates. Methods: 100 ESBL producing K. pneumoniae isolates were included in this study. Minimum inhibitory concentration (MIC) of imipenem was performed for all isolates by broth microdilution method. For CR-KP isolates, phenotypic detection of K. pneumoniae carbapenemase (KPC), metallo-β-lactamase (MBL) and AmpC enzymes was performed followed by Realtime qRT-PCR to detect and quantify ompK35 and ompK36 gene expression. Results: 42% of our isolates were c...