Haithm Hasan A H M E D Mousa (original) (raw)

Papers by Haithm Hasan A H M E D Mousa

Research paper thumbnail of Prediction of Overall Survival for Early-stage Non-small Cell Lung Cancer via a Novel 7-gene Prognostic Signature and Allele Frequency Deviation (AFD)

Research Square (Research Square), Sep 23, 2020

Background: Due to the late and poor prognosis of non-small lung cancer(NSCLC), the mortality of ... more Background: Due to the late and poor prognosis of non-small lung cancer(NSCLC), the mortality of patients is high, underlines the need to identify a credible prognostic marker for NSCLC patients. The aim of our study is to examine the association of allele frequency deviation (AFD) with the patient's survival, as well as identification and validation of a new prognostic signature to predict NSCLC overall survival(OS). Methods: First, we developed a new algorithm to calculate AFD from whole-exome sequencing(WES) data, then we compared the predictability of the patient's survival between AFD, tumor mutation burden (TMB) and change of variants allele frequency (dVAF). Second, we overlapped the differentially expressed genes (DEGs) from our data with the genes associated with the survival of The Cancer Genome Atlas (TCGA) database to confirm all genes significantly related to the survival of lung cancer. We identified 149 genes, 31 of which are new genes and have not been reported for lung cancer, that was used to develop a new prognostic model. Lung cancer adenocarcinoma (LUAD) data from the TCGA database was used to validate the gene-signature model. The prognostic model relating to the genes was established and validated in training and LUAD validation groups. Results: There was a significant association found between the high AFD value and poor survival among non-small cell lung cancer (NSCLC) patients. A novel seven genes (UCN2, RIMS2, CAVIN2, GRIA1, PKHD1L1, PGM5, CLIC6) were obtained through multivariate Cox regression analysis and significantly associated with NSCLC patients survival. Cox regression analysis confirmed that AFD and 7-gene signature are an independent prognostic marker in NSCLC patients. The AUC for 5-year survival in AFD and the AUC for 3-year survival in both training and validation groups were greater than 0.7. Conclusion: As a result, AFD and 7-gene signatures were identified as new independent predictive factors used for predicting the survival among NSCLC patients.

Research paper thumbnail of Rapid Progress in Diagnosis of ANCA-associated Vasculitis

Biomedical Journal of Scientific & Technical Research, 2018

Diagnosis of ANCA associated vasculitides (AAVs) represents a big burden in the medical field bec... more Diagnosis of ANCA associated vasculitides (AAVs) represents a big burden in the medical field because of clinical features diversity. Wegener's granulomatosis or granulomatosis with polyangiitis (GPA), Churg-Strauss syndrome or eosinophilic granulomatosis with polyangiitis (EGPA), and microscopic polyangiitis (MPA) are the main small-vessel vasculitis. GPA presents in the head and neck, and the Sinonasal cavity is among the most common areas affected. EPGA is occurring within patients with a history of asthma, whereas MPA is known by poor immune complexes markers in histopathology section. In addition, kidney and lung commonly involved in patients with MPA, this may sequentially involve the nervous and musculoskeletal systems. Moreover, skin, heart, eyes, and intestine can be involved. Due to of those varieties and overlapping in signs and symptoms the proper diagnosis is still an urgent need in AAVs management. Although detection of ANCA autoantibodies and histopathology reporting still essential tools for AAVs investigation, it has been reported that they are not enough for definitive diagnosis.

Research paper thumbnail of Prediction of Overall Survival for Early-stage Non-small Cell Lung Cancer via a Novel 7-gene Prognostic Signature and Allele Frequency Deviation (AFD)

Background Due to the late and poor prognosis of non-small lung cancer(NSCLC), the mortality of p... more Background Due to the late and poor prognosis of non-small lung cancer(NSCLC), the mortality of patients is high, underlines the need to identify a credible prognostic marker for NSCLC patients. The aim of our study is to examine the association of allele frequency deviation (AFD) with the patient's survival, as well as identification and validation of a new prognostic signature to predict NSCLC overall survival(OS).Methods First, we developed a new algorithm to calculate AFD from whole-exome sequencing(WES) data, then we compared the predictability of the patient's survival between AFD, tumor mutation burden (TMB) and change of variants allele frequency (dVAF). Second, we overlapped the differentially expressed genes (DEGs) from our data with the genes associated with the survival of The Cancer Genome Atlas (TCGA) database to confirm all genes significantly related to the survival of lung cancer. We identified 149 genes, 31 of which are new genes and have not been reported ...

Research paper thumbnail of Additional file 1 of Allele frequency deviation (AFD) as a new prognostic model to predict overall survival in lung adenocarcinoma (LUAD)

Additional file 1: Table S1. Source data of clinical information for patients with lung adenocarc... more Additional file 1: Table S1. Source data of clinical information for patients with lung adenocarcinoma (LUAD).

Research paper thumbnail of Additional file 2 of A seven-gene prognostic signature predicts overall survival of patients with lung adenocarcinoma (LUAD)

Additional file 2: Table S2: The 31 unreported prognostic genes associated with lung adenocarcino... more Additional file 2: Table S2: The 31 unreported prognostic genes associated with lung adenocarcinoma patients survival.

Research paper thumbnail of Additional file 1 of A seven-gene prognostic signature predicts overall survival of patients with lung adenocarcinoma (LUAD)

Additional file 1: Table S1: Source data underlying all figures in the analysis.

Research paper thumbnail of Additional file 4 of A seven-gene prognostic signature predicts overall survival of patients with lung adenocarcinoma (LUAD)

Additional file 4: Figure S2. The relationship between the gene expression of the seven genes in ... more Additional file 4: Figure S2. The relationship between the gene expression of the seven genes in the prognostic model and the KRAS mutation in LUAD patients.

Research paper thumbnail of Additional file 3 of A seven-gene prognostic signature predicts overall survival of patients with lung adenocarcinoma (LUAD)

Additional file 3: Figure S1. The relationship between the gene expression of the seven genes in ... more Additional file 3: Figure S1. The relationship between the gene expression of the seven genes in the prognostic model and the EGFR mutation in LUAD patients.

Research paper thumbnail of Additional file 5 of A seven-gene prognostic signature predicts overall survival of patients with lung adenocarcinoma (LUAD)

Additional file 5: Figure S3. Functional enrichment analysis of the seven prognostic genes associ... more Additional file 5: Figure S3. Functional enrichment analysis of the seven prognostic genes associated with overall survival in LUAD patients. (A) Biological process, (B) Cell component, (C) Molecular function, (D) KEGG pathway enrichment analysis. Dotplot indicates the counts of genes.

Research paper thumbnail of A seven-gene prognostic signature predicts overall survival of patients with lung adenocarcinoma (LUAD)

Cancer Cell International, 2021

Background Lung adenocarcinoma (LUAD) is one of the most common types in the world with a high mo... more Background Lung adenocarcinoma (LUAD) is one of the most common types in the world with a high mortality rate. Despite advances in treatment strategies, the overall survival (OS) remains short. Our study aims to establish a reliable prognostic signature closely related to the survival of LUAD patients that can better predict prognosis and possibly help with individual monitoring of LUAD patients. Methods Raw RNA-sequencing data were obtained from Fudan University and used as a training group. Differentially expressed genes (DEGs) for the training group were screened. The univariate, least absolute shrinkage and selection operator (LASSO), and multivariate cox regression analysis were conducted to identify the candidate prognostic genes and construct the risk score model. Kaplan–Meier analysis, time-dependent receiver operating characteristic (ROC) curve were used to evaluate the prognostic power and performance of the signature. Moreover, The Cancer Genome Atlas (TCGA-LUAD) dataset ...

Research paper thumbnail of Protective Effects of Cinnamaldehyde against Mesenteric Ischemia-Reperfusion-Induced Lung and Liver Injuries in Rats

Oxidative Medicine and Cellular Longevity, 2020

The aim of this study was to characterize and reveal the protective effects of cinnamaldehyde (CA... more The aim of this study was to characterize and reveal the protective effects of cinnamaldehyde (CA) against mesenteric ischemia-reperfusion- (I/R-) induced lung and liver injuries and the related mechanisms. Sprague-Dawley (SPD) rats were pretreated for three days with 10 or 40 mg/kg/d, ig of CA, and then induced with mesenteric ischemia for 1 h and reperfusion for 2 h. The results indicated that pretreatment with 10 or 40 mg/kg of CA attenuated morphological damage in both lung and liver tissues of mesenteric I/R-injured rats. CA pretreatment significantly restored the levels of aspartate transaminase (AST) and alanine transaminase (ALT) in mesenteric I/R-injured liver tissues, indicating the improvement of hepatic function. CA also significantly attenuated the inflammation via reducing myeloperoxidase (MOP) activity and downregulating the expression of inflammation-related proteins, including interleukin-6 (IL-6), interleukin-1β (IL-1β), cyclooxygenase-2 (Cox-2), and tumor necrosis...

Research paper thumbnail of Allele frequency deviation (AFD) as a new prognostic model to predict overall survival in lung adenocarcinoma (LUAD)

Cancer Cell International, 2021

Background Lung adenocarcinoma (LUAD) remains one of the world’s most known aggressive malignanci... more Background Lung adenocarcinoma (LUAD) remains one of the world’s most known aggressive malignancies with a high mortality rate. Molecular biological analysis and bioinformatics are of great importance as they have recently occupied a large area in the studies related to the identification of various biomarkers to predict survival for LUAD patients. In our study, we attempted to identify a new prognostic model by developing a new algorithm to calculate the allele frequency deviation (AFD), which in turn may assist in the early diagnosis and prediction of clinical outcomes in LUAD. Method First, a new algorithm was developed to calculate AFD using the whole-exome sequencing (WES) dataset. Then, AFD was measured for 102 patients, and the predictive power of AFD was assessed using Kaplan–Meier analysis, receiver operating characteristic (ROC) curves, and area under the curve (AUC). Finally, multivariable cox regression analyses were conducted to evaluate the independence of AFD as an in...

Research paper thumbnail of miR-421 up-regulation by the oleanolic acid derivative K73-03 regulates epigenetically SPINK1 transcription in pancreatic cancer cells leading to metabolic changes and enhanced apoptosis

Pharmacological Research, 2020

miR-421 up-regulation by the oleanolic acid derivative K73-03 regulates epigenetically SPINK1 tra... more miR-421 up-regulation by the oleanolic acid derivative K73-03 regulates epigenetically SPINK1 transcription in pancreatic cancer cells leading to metabolic changes and enhanced apoptosis

Research paper thumbnail of DC-SIGN mediates gastric cancer progression by regulating the JAK2/STAT3 signaling pathway and affecting LncRNA RP11-181G12.2 expression

Biomedicine & Pharmacotherapy, 2020

Background: The molecular mechanisms of gastric cancer (GC) development are very complicated. Rec... more Background: The molecular mechanisms of gastric cancer (GC) development are very complicated. Recent studies revealed that DC-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN)-related protein (DC-SIGNR) is involved in colon cancer and GC biological processes. However, the exact roles of DC-SIGN in GC remain unrevealed. Methods: DC-SIGN overexpression and knockdown experiments were performed by using DC-SIGN shRNA or DC-SIGN plasmid to investigate the biological roles of DC-SIGN in proliferation, cell cycle progression, migration and invasion of GC cells in vitro. Furthermore, the lncRNA profiles of SGC-7901 cells with control shRNA and DC-SIGN shRNA were generated by using microarray analysis. Mechanistically, the relationship between DC-SIGN, RP11-181G12.2 and the JAK2/STAT3 signaling pathway was then investigated using qRT-PCR and western blot assays. Additionally, we analyzed DC-SIGN and RP11-181G12.2 expression levels in GC specimens based on the Cancer Genome Atlas database. Results: In this study, the results showed that DC-SIGN was highly expressed in GC cells and significantly correlated with advanced clinical stage and lymphatic metastasis. Downregulation of DC-SIGN significantly inhibited the proliferation, cell cycle progression, migration and invasion of GC cells in vitro. The reverse results could partly be seen with the upregulation of DC-SIGN. Mechanistically, knockdown of DC-SIGN inactivated the JAK2/STAT3 signaling pathway, and overexpression of DC-SIGN activated the JAK2/STAT3 signaling pathway. In addition, through LncPath microarray analysis, we identified a lncRNA, RP11-181G12.2, that was significantly upregulated after knockdown of DC-SIGN; this was also confirmed by qRT-PCR. Furthermore, RP11-181G12.2 knockdown enhanced DC-SIGN expression in GC cells, further activating the JAK2/STAT3 signaling pathway. In contrast, DC-SIGN overexpression suppressed RP11-181G12.2 expression. Conclusions: Our study suggests that DC-SIGN might be involved in the progression of GC by regulating the JAK2/STAT3 signaling pathway and affecting lncRNA RP11-181G12.2 expression.

Research paper thumbnail of The immune-modulation and gut microbiome structure modification associated with long-term dietary supplementation of Lactobacillus rhamnosus using 16S rRNA sequencing analysis

Journal of Functional Foods, 2019

Probiotic dietary supplementation plays a crucial role in shaping gut microbiota and boost immune... more Probiotic dietary supplementation plays a crucial role in shaping gut microbiota and boost immune-responses. We herein investigated the potential effect of long-term dietary-supplementation of Lactobacillus rhamnosus (LBR) on gut microbiome structure, metabolome functions, and serum-profile. Our results revealed, LBR longterm supplementation reduces the body weight gain, ameliorates serum cytokines and attenuates serum lipoproteins profile. Moreover, LBR enhances biofilms-aerobics. Gut microbiome 16S rRNA analysis revealed LBR intervention significantly increased the relative abundance of Limnobacter, Turicibacterales, Enterococcus, and Vagococcus. On the contrary, the abundance of Helicobacteraceae, Rikenellaceae, Roseburia, Dorea, Anaerostipes, Coprococcus, Lachnospira, Oscillospira, Ruminococcaceae, Clotridiales, S24_7, Bacteroidia and Bacteroidetes found decreased in LBR treated group. Functional comparison revealed LBR exhibited higher level of energy production and conversion, amino acid transport and metabolism functions. Our findings demonstrate that the LBR dietary supplementation found to be beneficially modulating the immune-response, enhances lipoprotein metabolism, and ameliorates the microbial composition structure and functions.

Research paper thumbnail of MicroRNA-4316 inhibits gastric cancer proliferation and migration via directly targeting VEGF-A

Cancer Cell International, 2020

Background and aims microRNAs (miRNAs) have been reported to regulate proliferation and migration... more Background and aims microRNAs (miRNAs) have been reported to regulate proliferation and migration by down-regulating the expression of target genes. The aims of this study were to investigate whether miR-4316 inhibited proliferation and migration by downregulating vascular endothelial growth factor A (VEGF-A) and its clinical significance in gastric cancer (GC). Methods The clinical tissues of the GC patients for miR-4316 and VEGF-A were detected by qRT-PCR. The protein levels of VEGF-A and c-Met were determined by western blotting. Cell Proliferation, migration, and colony forming assays were conducted to show whether miR-4316 affects proliferation by CCK-8, migration by transwell, wound healing and colony formation assays. The bioinformatic methods and luciferase reporter assay were applied to detect the relationship between miRNA and VEGF-A on its targeting 3-untranslated regions (3-UTRs). CCK-8, colony formation, wound healing, and transwell assay were performed to explore the f...

Research paper thumbnail of Prediction of Overall Survival for Early-stage Non-small Cell Lung Cancer via a Novel 7-gene Prognostic Signature and Allele Frequency Deviation (AFD)

Research Square (Research Square), Sep 23, 2020

Background: Due to the late and poor prognosis of non-small lung cancer(NSCLC), the mortality of ... more Background: Due to the late and poor prognosis of non-small lung cancer(NSCLC), the mortality of patients is high, underlines the need to identify a credible prognostic marker for NSCLC patients. The aim of our study is to examine the association of allele frequency deviation (AFD) with the patient's survival, as well as identification and validation of a new prognostic signature to predict NSCLC overall survival(OS). Methods: First, we developed a new algorithm to calculate AFD from whole-exome sequencing(WES) data, then we compared the predictability of the patient's survival between AFD, tumor mutation burden (TMB) and change of variants allele frequency (dVAF). Second, we overlapped the differentially expressed genes (DEGs) from our data with the genes associated with the survival of The Cancer Genome Atlas (TCGA) database to confirm all genes significantly related to the survival of lung cancer. We identified 149 genes, 31 of which are new genes and have not been reported for lung cancer, that was used to develop a new prognostic model. Lung cancer adenocarcinoma (LUAD) data from the TCGA database was used to validate the gene-signature model. The prognostic model relating to the genes was established and validated in training and LUAD validation groups. Results: There was a significant association found between the high AFD value and poor survival among non-small cell lung cancer (NSCLC) patients. A novel seven genes (UCN2, RIMS2, CAVIN2, GRIA1, PKHD1L1, PGM5, CLIC6) were obtained through multivariate Cox regression analysis and significantly associated with NSCLC patients survival. Cox regression analysis confirmed that AFD and 7-gene signature are an independent prognostic marker in NSCLC patients. The AUC for 5-year survival in AFD and the AUC for 3-year survival in both training and validation groups were greater than 0.7. Conclusion: As a result, AFD and 7-gene signatures were identified as new independent predictive factors used for predicting the survival among NSCLC patients.

Research paper thumbnail of Rapid Progress in Diagnosis of ANCA-associated Vasculitis

Biomedical Journal of Scientific & Technical Research, 2018

Diagnosis of ANCA associated vasculitides (AAVs) represents a big burden in the medical field bec... more Diagnosis of ANCA associated vasculitides (AAVs) represents a big burden in the medical field because of clinical features diversity. Wegener's granulomatosis or granulomatosis with polyangiitis (GPA), Churg-Strauss syndrome or eosinophilic granulomatosis with polyangiitis (EGPA), and microscopic polyangiitis (MPA) are the main small-vessel vasculitis. GPA presents in the head and neck, and the Sinonasal cavity is among the most common areas affected. EPGA is occurring within patients with a history of asthma, whereas MPA is known by poor immune complexes markers in histopathology section. In addition, kidney and lung commonly involved in patients with MPA, this may sequentially involve the nervous and musculoskeletal systems. Moreover, skin, heart, eyes, and intestine can be involved. Due to of those varieties and overlapping in signs and symptoms the proper diagnosis is still an urgent need in AAVs management. Although detection of ANCA autoantibodies and histopathology reporting still essential tools for AAVs investigation, it has been reported that they are not enough for definitive diagnosis.

Research paper thumbnail of Prediction of Overall Survival for Early-stage Non-small Cell Lung Cancer via a Novel 7-gene Prognostic Signature and Allele Frequency Deviation (AFD)

Background Due to the late and poor prognosis of non-small lung cancer(NSCLC), the mortality of p... more Background Due to the late and poor prognosis of non-small lung cancer(NSCLC), the mortality of patients is high, underlines the need to identify a credible prognostic marker for NSCLC patients. The aim of our study is to examine the association of allele frequency deviation (AFD) with the patient's survival, as well as identification and validation of a new prognostic signature to predict NSCLC overall survival(OS).Methods First, we developed a new algorithm to calculate AFD from whole-exome sequencing(WES) data, then we compared the predictability of the patient's survival between AFD, tumor mutation burden (TMB) and change of variants allele frequency (dVAF). Second, we overlapped the differentially expressed genes (DEGs) from our data with the genes associated with the survival of The Cancer Genome Atlas (TCGA) database to confirm all genes significantly related to the survival of lung cancer. We identified 149 genes, 31 of which are new genes and have not been reported ...

Research paper thumbnail of Additional file 1 of Allele frequency deviation (AFD) as a new prognostic model to predict overall survival in lung adenocarcinoma (LUAD)

Additional file 1: Table S1. Source data of clinical information for patients with lung adenocarc... more Additional file 1: Table S1. Source data of clinical information for patients with lung adenocarcinoma (LUAD).

Research paper thumbnail of Additional file 2 of A seven-gene prognostic signature predicts overall survival of patients with lung adenocarcinoma (LUAD)

Additional file 2: Table S2: The 31 unreported prognostic genes associated with lung adenocarcino... more Additional file 2: Table S2: The 31 unreported prognostic genes associated with lung adenocarcinoma patients survival.

Research paper thumbnail of Additional file 1 of A seven-gene prognostic signature predicts overall survival of patients with lung adenocarcinoma (LUAD)

Additional file 1: Table S1: Source data underlying all figures in the analysis.

Research paper thumbnail of Additional file 4 of A seven-gene prognostic signature predicts overall survival of patients with lung adenocarcinoma (LUAD)

Additional file 4: Figure S2. The relationship between the gene expression of the seven genes in ... more Additional file 4: Figure S2. The relationship between the gene expression of the seven genes in the prognostic model and the KRAS mutation in LUAD patients.

Research paper thumbnail of Additional file 3 of A seven-gene prognostic signature predicts overall survival of patients with lung adenocarcinoma (LUAD)

Additional file 3: Figure S1. The relationship between the gene expression of the seven genes in ... more Additional file 3: Figure S1. The relationship between the gene expression of the seven genes in the prognostic model and the EGFR mutation in LUAD patients.

Research paper thumbnail of Additional file 5 of A seven-gene prognostic signature predicts overall survival of patients with lung adenocarcinoma (LUAD)

Additional file 5: Figure S3. Functional enrichment analysis of the seven prognostic genes associ... more Additional file 5: Figure S3. Functional enrichment analysis of the seven prognostic genes associated with overall survival in LUAD patients. (A) Biological process, (B) Cell component, (C) Molecular function, (D) KEGG pathway enrichment analysis. Dotplot indicates the counts of genes.

Research paper thumbnail of A seven-gene prognostic signature predicts overall survival of patients with lung adenocarcinoma (LUAD)

Cancer Cell International, 2021

Background Lung adenocarcinoma (LUAD) is one of the most common types in the world with a high mo... more Background Lung adenocarcinoma (LUAD) is one of the most common types in the world with a high mortality rate. Despite advances in treatment strategies, the overall survival (OS) remains short. Our study aims to establish a reliable prognostic signature closely related to the survival of LUAD patients that can better predict prognosis and possibly help with individual monitoring of LUAD patients. Methods Raw RNA-sequencing data were obtained from Fudan University and used as a training group. Differentially expressed genes (DEGs) for the training group were screened. The univariate, least absolute shrinkage and selection operator (LASSO), and multivariate cox regression analysis were conducted to identify the candidate prognostic genes and construct the risk score model. Kaplan–Meier analysis, time-dependent receiver operating characteristic (ROC) curve were used to evaluate the prognostic power and performance of the signature. Moreover, The Cancer Genome Atlas (TCGA-LUAD) dataset ...

Research paper thumbnail of Protective Effects of Cinnamaldehyde against Mesenteric Ischemia-Reperfusion-Induced Lung and Liver Injuries in Rats

Oxidative Medicine and Cellular Longevity, 2020

The aim of this study was to characterize and reveal the protective effects of cinnamaldehyde (CA... more The aim of this study was to characterize and reveal the protective effects of cinnamaldehyde (CA) against mesenteric ischemia-reperfusion- (I/R-) induced lung and liver injuries and the related mechanisms. Sprague-Dawley (SPD) rats were pretreated for three days with 10 or 40 mg/kg/d, ig of CA, and then induced with mesenteric ischemia for 1 h and reperfusion for 2 h. The results indicated that pretreatment with 10 or 40 mg/kg of CA attenuated morphological damage in both lung and liver tissues of mesenteric I/R-injured rats. CA pretreatment significantly restored the levels of aspartate transaminase (AST) and alanine transaminase (ALT) in mesenteric I/R-injured liver tissues, indicating the improvement of hepatic function. CA also significantly attenuated the inflammation via reducing myeloperoxidase (MOP) activity and downregulating the expression of inflammation-related proteins, including interleukin-6 (IL-6), interleukin-1β (IL-1β), cyclooxygenase-2 (Cox-2), and tumor necrosis...

Research paper thumbnail of Allele frequency deviation (AFD) as a new prognostic model to predict overall survival in lung adenocarcinoma (LUAD)

Cancer Cell International, 2021

Background Lung adenocarcinoma (LUAD) remains one of the world’s most known aggressive malignanci... more Background Lung adenocarcinoma (LUAD) remains one of the world’s most known aggressive malignancies with a high mortality rate. Molecular biological analysis and bioinformatics are of great importance as they have recently occupied a large area in the studies related to the identification of various biomarkers to predict survival for LUAD patients. In our study, we attempted to identify a new prognostic model by developing a new algorithm to calculate the allele frequency deviation (AFD), which in turn may assist in the early diagnosis and prediction of clinical outcomes in LUAD. Method First, a new algorithm was developed to calculate AFD using the whole-exome sequencing (WES) dataset. Then, AFD was measured for 102 patients, and the predictive power of AFD was assessed using Kaplan–Meier analysis, receiver operating characteristic (ROC) curves, and area under the curve (AUC). Finally, multivariable cox regression analyses were conducted to evaluate the independence of AFD as an in...

Research paper thumbnail of miR-421 up-regulation by the oleanolic acid derivative K73-03 regulates epigenetically SPINK1 transcription in pancreatic cancer cells leading to metabolic changes and enhanced apoptosis

Pharmacological Research, 2020

miR-421 up-regulation by the oleanolic acid derivative K73-03 regulates epigenetically SPINK1 tra... more miR-421 up-regulation by the oleanolic acid derivative K73-03 regulates epigenetically SPINK1 transcription in pancreatic cancer cells leading to metabolic changes and enhanced apoptosis

Research paper thumbnail of DC-SIGN mediates gastric cancer progression by regulating the JAK2/STAT3 signaling pathway and affecting LncRNA RP11-181G12.2 expression

Biomedicine & Pharmacotherapy, 2020

Background: The molecular mechanisms of gastric cancer (GC) development are very complicated. Rec... more Background: The molecular mechanisms of gastric cancer (GC) development are very complicated. Recent studies revealed that DC-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN)-related protein (DC-SIGNR) is involved in colon cancer and GC biological processes. However, the exact roles of DC-SIGN in GC remain unrevealed. Methods: DC-SIGN overexpression and knockdown experiments were performed by using DC-SIGN shRNA or DC-SIGN plasmid to investigate the biological roles of DC-SIGN in proliferation, cell cycle progression, migration and invasion of GC cells in vitro. Furthermore, the lncRNA profiles of SGC-7901 cells with control shRNA and DC-SIGN shRNA were generated by using microarray analysis. Mechanistically, the relationship between DC-SIGN, RP11-181G12.2 and the JAK2/STAT3 signaling pathway was then investigated using qRT-PCR and western blot assays. Additionally, we analyzed DC-SIGN and RP11-181G12.2 expression levels in GC specimens based on the Cancer Genome Atlas database. Results: In this study, the results showed that DC-SIGN was highly expressed in GC cells and significantly correlated with advanced clinical stage and lymphatic metastasis. Downregulation of DC-SIGN significantly inhibited the proliferation, cell cycle progression, migration and invasion of GC cells in vitro. The reverse results could partly be seen with the upregulation of DC-SIGN. Mechanistically, knockdown of DC-SIGN inactivated the JAK2/STAT3 signaling pathway, and overexpression of DC-SIGN activated the JAK2/STAT3 signaling pathway. In addition, through LncPath microarray analysis, we identified a lncRNA, RP11-181G12.2, that was significantly upregulated after knockdown of DC-SIGN; this was also confirmed by qRT-PCR. Furthermore, RP11-181G12.2 knockdown enhanced DC-SIGN expression in GC cells, further activating the JAK2/STAT3 signaling pathway. In contrast, DC-SIGN overexpression suppressed RP11-181G12.2 expression. Conclusions: Our study suggests that DC-SIGN might be involved in the progression of GC by regulating the JAK2/STAT3 signaling pathway and affecting lncRNA RP11-181G12.2 expression.

Research paper thumbnail of The immune-modulation and gut microbiome structure modification associated with long-term dietary supplementation of Lactobacillus rhamnosus using 16S rRNA sequencing analysis

Journal of Functional Foods, 2019

Probiotic dietary supplementation plays a crucial role in shaping gut microbiota and boost immune... more Probiotic dietary supplementation plays a crucial role in shaping gut microbiota and boost immune-responses. We herein investigated the potential effect of long-term dietary-supplementation of Lactobacillus rhamnosus (LBR) on gut microbiome structure, metabolome functions, and serum-profile. Our results revealed, LBR longterm supplementation reduces the body weight gain, ameliorates serum cytokines and attenuates serum lipoproteins profile. Moreover, LBR enhances biofilms-aerobics. Gut microbiome 16S rRNA analysis revealed LBR intervention significantly increased the relative abundance of Limnobacter, Turicibacterales, Enterococcus, and Vagococcus. On the contrary, the abundance of Helicobacteraceae, Rikenellaceae, Roseburia, Dorea, Anaerostipes, Coprococcus, Lachnospira, Oscillospira, Ruminococcaceae, Clotridiales, S24_7, Bacteroidia and Bacteroidetes found decreased in LBR treated group. Functional comparison revealed LBR exhibited higher level of energy production and conversion, amino acid transport and metabolism functions. Our findings demonstrate that the LBR dietary supplementation found to be beneficially modulating the immune-response, enhances lipoprotein metabolism, and ameliorates the microbial composition structure and functions.

Research paper thumbnail of MicroRNA-4316 inhibits gastric cancer proliferation and migration via directly targeting VEGF-A

Cancer Cell International, 2020

Background and aims microRNAs (miRNAs) have been reported to regulate proliferation and migration... more Background and aims microRNAs (miRNAs) have been reported to regulate proliferation and migration by down-regulating the expression of target genes. The aims of this study were to investigate whether miR-4316 inhibited proliferation and migration by downregulating vascular endothelial growth factor A (VEGF-A) and its clinical significance in gastric cancer (GC). Methods The clinical tissues of the GC patients for miR-4316 and VEGF-A were detected by qRT-PCR. The protein levels of VEGF-A and c-Met were determined by western blotting. Cell Proliferation, migration, and colony forming assays were conducted to show whether miR-4316 affects proliferation by CCK-8, migration by transwell, wound healing and colony formation assays. The bioinformatic methods and luciferase reporter assay were applied to detect the relationship between miRNA and VEGF-A on its targeting 3-untranslated regions (3-UTRs). CCK-8, colony formation, wound healing, and transwell assay were performed to explore the f...