Hamna qureshi - Academia.edu (original) (raw)

Hamna qureshi

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Papers by Hamna qureshi

Murine models of abdominal aortic aneurysms (AAA) are commonly used to study the pathogenesis of ... more Murine models of abdominal aortic aneurysms (AAA) are commonly used to study the pathogenesis of this disease. Rats are often used in laboratory studies because of their size and because they are more physiologically similar to humans than mice are. In this study, we subcutaneously implanted 9-week-old apolipoprotein E-deficient (apoE KO) rats with angiotensin II-filled pumps to study the effects of this vasoconstricting hormone on aneurysm development. Deletion of the apoE gene in mice causes excess lipid accumulation in the blood vessels, thereby increasing the likelihood of atherosclerotic lesions in the aorta. However, atherosclerotic plaque buildup was not evident when the rats consumed either normal chow or high fat diets, and no dissecting aneurysms were identified via ultrasound. We observed an average of 32.5 +/28.8 mmHg increase in systolic blood pressures as early as 4 days post-pump implantation and an increase of 54.2 +/25.9 mmHg from baseline 6 weeks after angiotensin ...

Journal of Vascular Research, 2017

The apolipoprotein E-deficient (apoE-/-) mouse model has advanced our understanding of cardiovasc... more The apolipoprotein E-deficient (apoE-/-) mouse model has advanced our understanding of cardiovascular disease mechanisms and experimental therapeutics. This spontaneous model recapitulates aspects of human atherosclerosis, and allows for the development of dissecting abdominal aortic aneurysms (AAAs) when combined with angiotensin II. We characterized apoE-/- rats and hypothesized that, similar to mice, they would develop dissecting AAAs. We created rats with a 16-bp deletion of the apoE gene using transcription activator-like effector nucleases. We imaged the suprarenal aorta for 28 days after implantation of miniosmotic pumps that infuse angiotensin II (AngII, 200 ng/kg/min). Blood pressure (BP), serum lipids and lipoproteins, and histology were also analyzed. These rats did not develop pathological aortic dissection, but we did observe a decrease in circumferential cyclic strain, a rise in BP, and microstructural changes in the aortic medial layer. We also measured increased seru...

Journal of Engineering and Science in Medical Diagnostics and Therapy, 2019

Preeclampsia leads to increased risk of morbidity and mortality for both mother and fetus. Most p... more Preeclampsia leads to increased risk of morbidity and mortality for both mother and fetus. Most previous studies have largely neglected mechanical compression of the left renal vein by the gravid uterus as a potential mechanism. In this study, we first used a murine model to investigate the pathophysiology of left renal vein constriction. The results indicate that prolonged renal vein stenosis after 14 days can cause renal necrosis and an increase in blood pressure (BP) of roughly 30 mmHg. The second part of this study aimed to automate a diagnostic tool, known as the supine pressor test (SPT), to enable pregnant women to assess their preeclampsia development risk. A positive SPT has been previously defined as an increase of at least 20 mmHg in diastolic BP when switching between left lateral recumbent and supine positions. The results from this study established a baseline BP increase between the two body positions in nonpregnant women and demonstrated the feasibility of an autonom...