Hanan Al-Ashaal - Academia.edu (original) (raw)

Papers by Hanan Al-Ashaal

Saponin rich fraction of Balanites fruits (SRF) was administered orally at a dose 250 mg/kg to sc... more Saponin rich fraction of Balanites fruits (SRF) was administered orally at a dose 250 mg/kg to schistosoma infected mice. Treatment with SRF showed amelioration signs in all biomarkers that confirmed by significant reduction in oogram, ova count and worm burden. Histopathological examination showed extensive reduction in granuloma sizes six weeks post treatment. Our results showed time dependant inhibition in the DNA damage induced in infected mice after treatment. Saponins were also successfully synthesized by callus cultures using Murashige and Skoog media. NMR analysis illustrated the presence of four major saponins of furostanol type from both fruits of natural plant and calli. Thus, SRF of B.aegyptiaca fruits possesses antischistomiasis activity. Antioxidant and antimutagenic activities could be considered as a possible mechanisms of action. Additionally, Balanites aegyptiaca saponins could be produced in continuous manner using in vitro cultures as future vision for drug produ...

Nickel is an essential trace element. Nickel and its compounds have many industrial applications.... more Nickel is an essential trace element. Nickel and its compounds have many industrial applications. Concern about nickel compounds is due to potential occupational and environmental health hazards. In the present study the mutagenic potential of nickel chloride (NiCl ) was tested to evaluate somatic and heritable 2 disorders which may extend to the next generations and associated with increased risk of cancer. The protective role of dietary soybean seeds and its extracts was also evaluated. The study examined the induction of chromosomal aberrations in bone marrow cells and mouse spermatocytes and the effect on morphological sperm abnormalities. The results showed that NiCl induced an increase in the percentage of chromosomal aberrations 2 in somatic and germ cells with dose and time relationship. A significant (P<0.01) increase in the percentage of abnormal sperm was observed after treatment with the tested doses. The results also demonstrated that soybean seeds and its extracts h...

European Scientific Journal, ESJ, 2015

Saponin rich fraction of Balanites fruits (SRF) was administered orally at a dose of 250 mg/kg to... more Saponin rich fraction of Balanites fruits (SRF) was administered orally at a dose of 250 mg/kg to schistosoma infected mice. Treatment with SRF showed amelioration signs in all biomarkers that confirmed by significant reduction in oogram, ova count and worm burden. Histopathological examination showed extensive reduction in granuloma sizes after 6 weeks treatment. Our results showed time dependant inhibition in the DNA damage induced in infected mice after treatment. Saponins were also successfully synthesized by callus cultures using Murashige and Skoog media. NMR analysis illustrated the presence of 4 major saponins of furostanol type from both fruits of natural plant and calli. Thus, SRF of B.aegyptiaca fruits possesses antischistomiasis activity. Antioxidant and antimutagenic activities could be considered as possible mechanisms of action. Additionally, Balanites aegyptiaca saponins could be produced in continuous manner using in vitro cultures as future vision for drug producti...

This review deals with microorganisms that are associated with the human body, their types and di... more This review deals with microorganisms that are associated with the human body, their types and diversity. The survey also covers the symbiotic and adverse effects of the located flora and how could friendly microbial communities change to vigorous pathogenic enemies. Besides, the study includes special oversight on the natural compounds that may help to overcome serious diseases that are caused by or that result from microbiome alteration or dysbiosis.

Asian Pacific Journal of Tropical Disease, Nov 27, 2017

To investigate the biological activities of polar and non-polar extracts of Balanites aegyptiaca ... more To investigate the biological activities of polar and non-polar extracts of Balanites aegyptiaca fruits. Methods: Antihyperglycemic activity using alloxan induced diabetic rats was evaluated. In vitro cytotoxicity against human carcinoma cell lines activity in addition to antioxidant and antitrematodal effects were investigated. Phytochemicals were determined using chromatographic and spectral analyses including TLC, GC and LC/MS/MS methods. Results: The reduction in blood glucose level reached 64.13%, 69.07% and 77.01% for hexane, chloroform and methanol extract, respectively. Isolated organs and histopathological examination illustrated improvement in treated animal's pancreas which is the master gland in controlling glucose level. The highest in vitro free radical scavenging capacity was achieved by chloroform extract (75.72%). Meanwhile, hexane and methanol extracts exhibited 44.01% and 41.77% scavenging capacity, respectively. Cytotoxic activity against human carcinoma cell lines illustrated efficacious influence against brain, liver, lung, breast and lymphoblastic leukemia cell lines. Brain cell line was the most susceptible cell line by both chloroform and methanol extracts. In vitro antitrematodal effectiveness showed that 100% mortality of Schistosoma worms was induced at the 3rd and 5th days for methanol and chloroform extracts. Meanwhile, both extracts exhibited antifasciolosis activity with LC 50 of 63.19 and 55.15 mg/L, respectively. Conclusions: The current results illustrated that Balanites aegyptiaca phytochemicals induced potent hypoglycemic activity and potent to moderate antioxidant and cytotoxic effects. Chloroform and methanol extracts were found to have antitrematodal efficacy against Schistosoma mansoni and Fasciola gigantica hepatic worms.

Comunicata Scientiae, 2019

The target of this study is production of glycoalkaloids from cultures of Solanum nigrum var. jud... more The target of this study is production of glycoalkaloids from cultures of Solanum nigrum var. judaicum Besser. Further, to evaluate their therapeutic effects. S. nigrum var. judaicum leaves were implanted in MS media containing growth regulators for in vitro study. HPLC analyses were applied for qualitative and quantitative determination of glycoalkaloids. Cytotoxic effects against human carcinoma cell lines were evaluated. In addition, antiviral, antioxidant, anti-inflammatory and antiparasitic activities of the formed glycoalkaloids were estimated. HPLC data indicated the success of in vitro solasodine and solanidine glycosides production. Solasonine represented the highest concentration. Biological assays illustrated that obtained glycoalkaloids exhibited cytotoxic activity against human carcinoma cell lines that may be attributed to free radical scavenging activity (69.98%). Strong antiherps performance was observed (94%). In addition, the glycoalkaloids showed in vitro schisto...

The Natural Products Journal, 2017

Egyptian Pharmaceutical Journal, 2013

Objective The aim of the present work was to study the production of tropane alkaloids by in vitr... more Objective The aim of the present work was to study the production of tropane alkaloids by in vitro cultures of Atropa belladonna L. and to evaluate the anticonvulsant, antinociceptive, motor incoordination, and antioxidant activities of both in vitro and original plant extracts. Background A. belladonna is a very important medicinal plant with multipurpose therapeutic effects. The yield of its alkaloid content is very low, which makes it difficult for industrial application. Materials and methods Murashige and Skoog media were used for callus and plant differentiation induction from leaf explants of A. belladonna L. Qualitative and quantitative analysis of alkaloids was carried out using high-performance liquid chromatography. The anticonvulsant activity was screened by the pentylenetetrazole seizure test. The antinociceptive activity was evaluated by adopting the writhing test, whereas motor incoordination was evaluated using the rotarod test. In addition, antioxidant activity was estimated using the 2,2'-diphenyl-1-picrylhydrazyl radical-scavenging test. Results Callus and differentiated plants were successfully induced in Murashige and Skoog media supplemented with growth regulators. High-performance liquid chromatography analysis revealed the production of higher concentrations of tropane alkaloids in differentiated plants than in the original plant. Anticonvulsant and antinociceptive activities, motor incoordination, and the antioxidant effect of callus extracts were much higher than those of the original plant leaf extract. Conclusion Plant tissue culture could be considered as an efficient and alternative source of continuous supply of tropane alkaloids with potent anticonvulsant, antinociceptive, motor incoordination, and antioxidant activities. It is also a powerful tool for producing A. belladonna strain with a high tropane alkaloid content.

Toxicological & Environmental Chemistry, 2012

Aim of study: Evaluation of the protective role of Glycine max L. (soybean) against aluminium sul... more Aim of study: Evaluation of the protective role of Glycine max L. (soybean) against aluminium sulfate -induced genotoxicityA diet containing 30% soybean and extracts obtained with various organic solvents were administered orally to male white Swiss mice prior to intraperitoneal injection of Al2(SO4)3. Light microscopy was used for examination of genotoxicity involving somatic cells, germ cells, and sperms. The main

Journal of Ethnopharmacology, 2010

Ethnopharmacological relevance: Balanites aegyptiaca (Balantiaceae), mainly the fruit, is used by... more Ethnopharmacological relevance: Balanites aegyptiaca (Balantiaceae), mainly the fruit, is used by traditional healers and herbalists for treating many diseases in Africa and Asia. Aim of the study: Investigation of fixed oil composition of fruits and evaluation of its biological activity. Materials and methods: Oil content was identified using GC and GC/MS. In vitro examination of the oil biological activity (including cytotoxicity, antimutagenicity, antiparasitic, antiviral and antimicrobial activities) was performed. Results: The oil contained 54.53% unsaturated fatty acids and 1.14% sterols. The oil exhibited anticancer activity against lung, liver and brain human carcinoma cell lines. It also had antimutagenic activity against Fasciola gigantica induced mutagenicity besides anthelmintic activity against hepatic worms (Schistosoma mansoni and Fasciola gigantica). Preliminary screening showed that the oil had antiviral activity against Herpes simplex virus. It also had antimicrobial activity against selected strains of Gram-positive bacteria, Gram-negative bacteria and Candida. Conclusion: The results showed remarkable biological activity of Balanites aegyptiaca fixed oil and proved its importance as natural bioactive source.

Pharmaceutical biology, 2011

Hesperidin is a flavonoid that has various pharmacological activities including anti-inflammatory... more Hesperidin is a flavonoid that has various pharmacological activities including anti-inflammatory, antimicrobial and antiviral activities.

Asian Pacific Journal of Tropical Disease, 2017

All experimental procedures involving animals were conducted in accordance to the approved guidel... more All experimental procedures involving animals were conducted in accordance to the approved guidelines of the Ethical Committee of the National Research Center for use and care of experimental animals. The journal implements double-blind peer review practiced by specially invited international editorial board members. Objective: To evaluate the anti schistosomiasis activity of the bioactive formed glycoalkaloids from Solanum nigrum L. (family: Solanaceae) (S. nigrum) to control one of the most prevalent parasitic tropical diseases. Methods: Murashige and Skoog media containing growth regulators were used for callus and regeneration establishment. High performance liquid chromatography analysis was used for identification and quantitation of the glycoalkaloids. Mice infected with cercariae were used for biological studies. Hepatic marker enzymes, urea cycle enzymes and antioxidant biomarkers as well as chromosomal aberrations of mice were measured before and after treatment. Histopathological examination for infected and treated mice was also carried out. Results: High performance liquid chromatography analysis proved that S. nigrum cultures had the power to produce glycoalkaloids from calli and regenerate plants in higher concentrations than original plant. Treatment of infected mice with the separated glycoalkaloids induced significant improvement of all tested biomarkers. In addition, glycoalkaloids administration resulted in significant elaboration of somatic and germ cell mutation caused by bilharzia worms. Histopathological study illustrated improvement signs regarding inflammation and egg disintegrations. The refinement of biological signs was dose dependent. Conclusions: The outcomes of this study indicated potential effect of in vitro cultures of S. nigrum for glycoalkaloids formation. The data proved the potent effects of the glycoalkaloids against the hazards of bilharzias' infection including liver, renal and chromosomal disorders. The data of the present study could be a tool for development of plant originated antibilharziasis medicine to dispose the danger of ultimate debilitating helminthes.

Solanum tuberosum L. on MS media containing BA, IAA, and Kin. Glycoalkaloids are produced in call... more Solanum tuberosum L. on MS media containing BA, IAA, and Kin. Glycoalkaloids are produced in callus and shoots in concentrations higher than original tubers using HPLC. Callus methanolic extract had promising anticancer activity with low IC 50 values against human carcinoma cell lines of breast, lymphoplastic leukemia, larynx, liver, cervix, colon, and brain, IC 50 (µg/mL) were 2.