Hanifah Yusuf - Academia.edu (original) (raw)
Papers by Hanifah Yusuf
Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences
The purpose of this study was to analyze how the effect of each increase in Body Mass Index (BMI)... more The purpose of this study was to analyze how the effect of each increase in Body Mass Index (BMI) on the increase in blood pressure (BP). The research design was a laboratory experiment with 52 non-obese and obese rats as subjects (non-obese, n=14 and obese, n=38). Rats were induced obesity using a high-fat diet for four weeks. BMI used is based on the Lee Index with obesity indicators if 300 g/cm. Data analysis using independent sample t-test, simple linear regression test, and Pearson correlation test; (p0.05). The results: BP in non-obese rats was lower than obese rats (89.00±7.38 vs 190.11±4.42; p=0.001*), BMI was positively associated with BP (p=0.001*); an increase in BMI will increase BP (p=0.001*), there is a strong correlation between BMI and BP (r=0.977; p=0.001*). The regression equation (Y = 707.42 + 2.75 X) indicates that every 1 g/cm increase in BMI will be followed by an increase in BP of 2.75 mmHg. The analysis of R-square = 0.942 shows that the accuracy of the linea...
Open Access Macedonian Journal of Medical Sciences, 2022
BACKGROUND: The effect of green coffee (GC) on blood pressure (BP) is still debated, but GC is th... more BACKGROUND: The effect of green coffee (GC) on blood pressure (BP) is still debated, but GC is thought to improve liver and kidney function. AIM: This study aimed to analyze the effect of the GC intervention on BP, liver, and kidney functions in obese model rats. METHODS: The research was a pre-clinical trial of pretest-posttest with control group design. Animals were divided into four groups: obese rats (G1), obese rats and GC (G2), obese rats and physical exercise (PE) (G3), and a combination of interventions (PE+GC) (G4). Data analysis used an independent sample t-test and analysis of variance; (p < 0.05). RESULTS: There was a different effect of the GC, PE, and PE+GC intervention on BP (186.50 ± 3.45 vs. 91.33 ± 1.96 p = 0.001*; 189.17 ± 2.93 vs. 119.50 ± 3.73 p = 0.001*; 191.8 3 ± 2.64 vs. 98.83 ± 3.76 p = 0.001*) in obese rats. There was a significant difference in Serum Glutamic Oxaloacetic Transaminase (SGOT) (p=0.001*), Serum Glutamic Pyruvic Transaminase (SGPT) (p = 0.0...
Pharmacia
This research was aimed at analyzing the acute effects of Arabica black coffee consumption on blo... more This research was aimed at analyzing the acute effects of Arabica black coffee consumption on blood glucose, insulin, and serum cortisol levels, as well as determining the pharmacological effects of black coffee as an antihyperglycemic. A randomized control trial with healthy female subjects was used in this study. There were 20 volunteers in total: 9 as the control group and 11 as the trial group. The treatment included brewing 10 grams of Gayo Arabica black coffee powder with 150 ml of boiling water. Blood glucose, insulin, and cortisol levels were measured twice, before and after 60 minutes of coffee consumption. An independent sample t-test (p < 0.05), Pearson correlation test (p < 0.05), and simple linear correlation test (p < 0.05) were used to analyze the data. Blood glucose levels and serum cortisol levels decreased significantly after coffee consumption in the trial group (p = 0.002* and p = 0.001*). There was no significant negative correlation between glucose and...
Budapest International Research and Critics Institute (BIRCI-Journal): Humanities and Social Sciences, 2020
The pattern of disease events at this time has experienced a change marked by changes in disease ... more The pattern of disease events at this time has experienced a change marked by changes in disease and death that were originally dominated by infectious diseases switch to non-communicable diseases. In the 2018 Riskesdas results the prevalence of hypertension in Aceh increased compared to 2013. Purpose: to find out the factors associated with the incidence of hypertension in women at the Baiturrahman Health Center in Banda Aceh City. Research Methods: This research is a type of quantitative research with cross-sectional study design conducted on 124 samples. Sampling was carried out using the Accidental Sampling technique. Data analysis uses univariate, bivariate and multivariate. Statistical analysis using chi-square and logistic regression. Results: The results showed that there was a significant relationship between family history (p-value = 0.007, OR = 2.8), food consumption patterns (p-value = 0.025, OR = 2.5), obesity status (p-value = 0.046, OR = 2.2), Physical activity (p-val...
Open Access Macedonian Journal of Medical Sciences, 2021
BACKGROUND: Diabetes mellitus (DM) continues to increase with currently more than 463 million peo... more BACKGROUND: Diabetes mellitus (DM) continues to increase with currently more than 463 million people in the world live with DM. One of the causes of Type 2 DM (T2DM) is insulin resistance. Determining laboratory-based tests for early identification of T2DM is important. One of the tests developed was the detection of homeostatic model assessment – insulin resistance (HOMA-IR) levels. AIM: Using chlorogenic acid found in coffee leaves as antidiabetic agent for alternative treatments in DM, this research is conducted to understand the effect of Coffee Arabica Gayo Leaf Extract (Coffea arabica L.) in decreasing HOMA-IR levels in T2DM rats. MATERIALS AND METHODS: Thirty-five male Wistar rats (Rattus norvegicus) had T2DM induced using a combination of feeding a high-fat diet for 5 weeks followed by multiple intraperitoneal injections of low-dose streptozotocin (30 mg/kgBW), divided into 7 groups (2 groups that did not receive treatment [K1 and K3] and 5 groups that received treatment [K2...
Journal of Research in Pharmacy, 2022
Mangiferin is a bioactive compound which has antioxidant effect, but its effect on liver protecti... more Mangiferin is a bioactive compound which has antioxidant effect, but its effect on liver protection during chemotherapy with doxorubicin is understudied. The objective of this study was to assess the protective effect of mangiferin on liver injury induced by doxorubicin. The doxorubicin was injected intraperitoneally at a total dose of 15 mg/kg b.w, and then the animals were orally supplemented with mangiferin (either 50 and 100 mg/kg b.w) for five weeks. The activity of alanine aminotransferase (ALT), aspartate aminotransferase (AST), superoxide dismutase (SOD), the level of malondialdehyde (MDA) and glutathione, and liver histopathology were examined to evaluate the liver damage. The results showed that doxorubicin could cause liver cell damage to the rats. In addition, the study revealed that the supplementation of mangiferin (50 and 100 mg/kg b.w) decreased ALT and AST activities, as well as lipid peroxidation. It also increased SOD and GSH levels of liver cells (p <0.05). Our study proved that the improvement in liver function and oxidative stress parameters caused by doxorubicin, indicates that the mangiferin has a protective effect against liver damage induced by doxorubicin.
Asian Pacific Journal of Cancer Prevention, 2022
Background: Cervical cancer is the leading cause of cancer death in women. Chemotherapy is one of... more Background: Cervical cancer is the leading cause of cancer death in women. Chemotherapy is one of the treatment modalities with many side effects. This study aimed to evaluate the anticancer activity of soluble ethyl acetate partition of Chromolaena odorata (C. odorata) leaves on HeLa cells of cervical cancer. Material and methods: The cytotoxicity activity of the soluble ethyl acetate extract of the C. odorata leaves was analyzed by MTT colorimetry assay. The apoptosis activity was determined by double staining and flow cytometry techniques. Doubling time assay was used to observed HeLa cells proliferation. Results: The IC 50 of soluble ethyl acetate partition of this plant was 82.41± 6.73 µg/ml against HeLa cells. The apoptosis activity showed that HeLa cells underwent morphological changes in dosedependent manner while the highest number of dead cells was observed after treatment with 500 µg/ml of the partition. Flow cytometry analysis showed that treatment with IC 50 and 2x IC 50 resulted in death of more than 97% cells (p-value <0.05 in both comparisons). Proliferation of HeLa cells was also inhibited following treatment with ½ IC50, IC50, and 2xIC 50 in the first 24 hours (p-value <0.05 in all comparison). Conclusion: The findings of this study suggested that the soluble ethyl acetate partition from ethanol extract of C. odorata leaves had cytotoxic and antiproliferative properties against HeLa cells.
Buku ini membahas malaria dalam kaitannya dengan tantangan dan peluang untuk menemukan dan mengem... more Buku ini membahas malaria dalam kaitannya dengan tantangan dan peluang untuk menemukan dan mengembangkan senyawa baru dari bahan alam sebagai antimalaria, sehingga dapat membantu mengatasi permasalahan resistensi Plasmodium yang semakin meluas. Buku ini untuk pengembangan pengetahuan tentang upaya preventif malaria berbasis bahan alam yang menggambarkan secara molekuler peran Eurikumanon yang terlibat pada pathogenesis pengobatan malaria. Hadirnya buku ini mengajak pembaca akan meningkatkan inovasi dan motivasi untuk mencari pola baru dalam menuntaskan malaria berbasis bahan alam.
![Research paper thumbnail of The anticancer activity of ethanol extract of Chromolaena odorata leaves in 7,12-Dimethylbenz[a]anthracene in (DMBA) induced breast cancer Wistar rats (Rattus novergicus)](https://attachments.academia-assets.com/113367571/thumbnails/1.jpg)
](Pharmacia, 2021
Background: Breast cancer chemotherapy with standard drugs such as doxorubicin will induce cardio... more Background: Breast cancer chemotherapy with standard drugs such as doxorubicin will induce cardiotoxicity. Therefore, this study aims to evaluate the anticancer activity ofC. odorataleaves extract in DMBA induced breast cancer on rats.Methods: Seven groups ofRattus novergicuswere used: Four treatment groups ofC. odorataextract (500, 1000, 2000, and 4000 mg/kg BW), normal control, breast cancer control, and doxorubicin treatment group. The number, volume, and weight of the nodule and the rats’ body weight were compared among groups. Data was analyzed using paired t-test or one-way ANOVA with post hoc analysis as appropriate.Results: Significant decline of the number, volume, and weight of cancer nodules was observed in the treatment group (p< 0.001). The weight of the cancer nodule at week 16thwas also significantly reduced in GCo2000compared to Gdoxo(p< 0.0001). A significant increase in body weight was also dose-dependent, especially at week 11th(p< 0.05 in all comparisons...
Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition), 2020
Background: Coffee contains caffeine. Caffeine is the main component that influences the response... more Background: Coffee contains caffeine. Caffeine is the main component that influences the response of the cardiovascular system and blood pressure (BP). Acute response of coffee to increased BP is related to caffeine.Objectives: This study aims to analyze the acute response of coffee consumption on BP in healthy females therefore coffee is an alternative therapy for hypotension.Methods: The research design was a clinical trial. Treatment: black coffee, Gayo’s Arabica, 10 mg, coffee brewed with 150 ml of boiling water, without sugar. BP was examined using a mercury sphygmomanometer and stethoscope. BP each subject was examined twice and averaged. BP was checked 3 times: before, 30, and 60 minutes after coffee consumption. All subjects were non-coffee drinkers. A total of 20 healthy female, 18-20 years old were divided into two groups: the non-intervention (n=9) and intervention (n=11). Data was analyzed by independent and paired sample t-test.Results: Coffee increased systolic 10-20 m...
Narra J, 2021
This study was conducted to determine the prevalence of prolonged neuromuscular symptoms, includi... more This study was conducted to determine the prevalence of prolonged neuromuscular symptoms, including fatigue, anosmia, headache, myalgia, and joint pain in COVID-19 survivors hospitalized with mild, moderate, or severe infections worldwide. The search was conducted up to January 30th, 2021 using three databases (PubMed, Scopus, and Web of Science) to identify potentially eligible studies. Data on study characteristics, follow-up characteristics, and severity of COVID-19 during hospitalization were collected in accordance with PRISMA guidelines. The Newcastle-Ottawa scale was used to assess the quality of relevant articles. The estimated prevalence of specific prolonged neuromuscular symptoms and the association between COVID-19 severity and occurrence of prolonged neuromuscular symptoms was analyzed wherever appropriate. Database search yielded 4,050 articles and 22 articles were included for meta-analysis. The estimated prevalence of prolonged fatigue was recorded in 21.2% (95%CI: 1...
Doxorubicin (DOXO) is an anticancer that is often used clinically which induces cardiotoxicity in... more Doxorubicin (DOXO) is an anticancer that is often used clinically which induces cardiotoxicity in the patient. Therefore, finding a new compound that acts as co-chemotherapy is a current challenge. Eurycomanone (EURY) isolated from Eurycoma longifolia Jack have been shown to have anticancer activity in several studies. This study aimed to observe the effect of EURY, DOXO, and its combination on the cell cycle of T47D and MCF-7 breast cancer cell lines. Anticancer activities of EURY and DOXO were examined by MTT colorimetry assay. The effect of combination was analyzed by the Combination Index (CI) method using Compusyn software. The apoptotic activity was analyzed using flow cytometry. To determine CI, a fixed concentration ratio between EURY and DOXO was used from the IC50 value at concentrations (1/2; 1/4; 1/8; 1/16 of IC50). We found that EURY had IC50 values at 0.377μg/mL on T47D and 4.7μg/mL on MCF-7 cells, while DOXO had IC50 values 1.845μg/mL on T47D and 5.074μg/mL on MCF-7ce...
Diabetes poses a public health burden range from the inadequacy of current therapies to complicat... more Diabetes poses a public health burden range from the inadequacy of current therapies to complications. A new diabetic therapy derived from plants with less or no adverse effects is needed. Chromolaena odorata is commonly used as a traditional antidiabetic agent in some countries. The objective of this study was to evaluate the antidiabetic activity of C. odorata leaves extract in diabetic-induced rats. Six groups of Rattus novergicus were used: three concentrations of C. odorata leave extracts (100mg/kg body weight (BW), 200mg/kg BW, and 400mg/kg BW), normal control, diabetic control, and glibenclamide treatment group. C. odorata extract was administrated orally each day for five weeks. The animal body weight, blood glucose level, insulin level, and pathological changes of the pancreas were compared among groups. Data were analyzed using paired t-test or one-way ANOVA with post hoc analysis as appropriate. Our data indicated a significant difference in body weight between the diabet...
Open Access Macedonian Journal of Medical Sciences, 2021
BACKGROUND: Diabetes mellitus (DM) is a chronic and progressive metabolic disease characterized b... more BACKGROUND: Diabetes mellitus (DM) is a chronic and progressive metabolic disease characterized by hyperglycemia due to impaired insulin secretion associated with a lack of phosphoinositide 3-kinase (PI3K) and glucose transporter-4 (GLUT-4) expression in the skeletal muscle membrane. AIM: The aim of the study is to understand the effect of coffee Arabica Gayo leaf extract (Coffea arabica L.) in increasing PI3K expression and GLUT-4 expression in the skeletal muscle membrane. METHODS: Thirty-five male Wistar rats with Type 2 DM (T2DM) induced using a combination of a high-fat diet for 5 weeks followed by multiple intraperitoneal injections of low-dose streptozotocin (30 mg/kg). Divided into seven groups as such two groups that did not receive treatment and five groups that received treatment. The dosage administered was 150, 200, and 250 mg/kg/day through the nasogastric tube for 30 days. PI3K and GLUT-4 expression in the skeletal muscle membrane was evaluated by Immunohistochemistry...
Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences
The purpose of this study was to analyze how the effect of each increase in Body Mass Index (BMI)... more The purpose of this study was to analyze how the effect of each increase in Body Mass Index (BMI) on the increase in blood pressure (BP). The research design was a laboratory experiment with 52 non-obese and obese rats as subjects (non-obese, n=14 and obese, n=38). Rats were induced obesity using a high-fat diet for four weeks. BMI used is based on the Lee Index with obesity indicators if 300 g/cm. Data analysis using independent sample t-test, simple linear regression test, and Pearson correlation test; (p0.05). The results: BP in non-obese rats was lower than obese rats (89.00±7.38 vs 190.11±4.42; p=0.001*), BMI was positively associated with BP (p=0.001*); an increase in BMI will increase BP (p=0.001*), there is a strong correlation between BMI and BP (r=0.977; p=0.001*). The regression equation (Y = 707.42 + 2.75 X) indicates that every 1 g/cm increase in BMI will be followed by an increase in BP of 2.75 mmHg. The analysis of R-square = 0.942 shows that the accuracy of the linea...
Open Access Macedonian Journal of Medical Sciences, 2022
BACKGROUND: The effect of green coffee (GC) on blood pressure (BP) is still debated, but GC is th... more BACKGROUND: The effect of green coffee (GC) on blood pressure (BP) is still debated, but GC is thought to improve liver and kidney function. AIM: This study aimed to analyze the effect of the GC intervention on BP, liver, and kidney functions in obese model rats. METHODS: The research was a pre-clinical trial of pretest-posttest with control group design. Animals were divided into four groups: obese rats (G1), obese rats and GC (G2), obese rats and physical exercise (PE) (G3), and a combination of interventions (PE+GC) (G4). Data analysis used an independent sample t-test and analysis of variance; (p < 0.05). RESULTS: There was a different effect of the GC, PE, and PE+GC intervention on BP (186.50 ± 3.45 vs. 91.33 ± 1.96 p = 0.001*; 189.17 ± 2.93 vs. 119.50 ± 3.73 p = 0.001*; 191.8 3 ± 2.64 vs. 98.83 ± 3.76 p = 0.001*) in obese rats. There was a significant difference in Serum Glutamic Oxaloacetic Transaminase (SGOT) (p=0.001*), Serum Glutamic Pyruvic Transaminase (SGPT) (p = 0.0...
Pharmacia
This research was aimed at analyzing the acute effects of Arabica black coffee consumption on blo... more This research was aimed at analyzing the acute effects of Arabica black coffee consumption on blood glucose, insulin, and serum cortisol levels, as well as determining the pharmacological effects of black coffee as an antihyperglycemic. A randomized control trial with healthy female subjects was used in this study. There were 20 volunteers in total: 9 as the control group and 11 as the trial group. The treatment included brewing 10 grams of Gayo Arabica black coffee powder with 150 ml of boiling water. Blood glucose, insulin, and cortisol levels were measured twice, before and after 60 minutes of coffee consumption. An independent sample t-test (p < 0.05), Pearson correlation test (p < 0.05), and simple linear correlation test (p < 0.05) were used to analyze the data. Blood glucose levels and serum cortisol levels decreased significantly after coffee consumption in the trial group (p = 0.002* and p = 0.001*). There was no significant negative correlation between glucose and...
Budapest International Research and Critics Institute (BIRCI-Journal): Humanities and Social Sciences, 2020
The pattern of disease events at this time has experienced a change marked by changes in disease ... more The pattern of disease events at this time has experienced a change marked by changes in disease and death that were originally dominated by infectious diseases switch to non-communicable diseases. In the 2018 Riskesdas results the prevalence of hypertension in Aceh increased compared to 2013. Purpose: to find out the factors associated with the incidence of hypertension in women at the Baiturrahman Health Center in Banda Aceh City. Research Methods: This research is a type of quantitative research with cross-sectional study design conducted on 124 samples. Sampling was carried out using the Accidental Sampling technique. Data analysis uses univariate, bivariate and multivariate. Statistical analysis using chi-square and logistic regression. Results: The results showed that there was a significant relationship between family history (p-value = 0.007, OR = 2.8), food consumption patterns (p-value = 0.025, OR = 2.5), obesity status (p-value = 0.046, OR = 2.2), Physical activity (p-val...
Open Access Macedonian Journal of Medical Sciences, 2021
BACKGROUND: Diabetes mellitus (DM) continues to increase with currently more than 463 million peo... more BACKGROUND: Diabetes mellitus (DM) continues to increase with currently more than 463 million people in the world live with DM. One of the causes of Type 2 DM (T2DM) is insulin resistance. Determining laboratory-based tests for early identification of T2DM is important. One of the tests developed was the detection of homeostatic model assessment – insulin resistance (HOMA-IR) levels. AIM: Using chlorogenic acid found in coffee leaves as antidiabetic agent for alternative treatments in DM, this research is conducted to understand the effect of Coffee Arabica Gayo Leaf Extract (Coffea arabica L.) in decreasing HOMA-IR levels in T2DM rats. MATERIALS AND METHODS: Thirty-five male Wistar rats (Rattus norvegicus) had T2DM induced using a combination of feeding a high-fat diet for 5 weeks followed by multiple intraperitoneal injections of low-dose streptozotocin (30 mg/kgBW), divided into 7 groups (2 groups that did not receive treatment [K1 and K3] and 5 groups that received treatment [K2...
Journal of Research in Pharmacy, 2022
Mangiferin is a bioactive compound which has antioxidant effect, but its effect on liver protecti... more Mangiferin is a bioactive compound which has antioxidant effect, but its effect on liver protection during chemotherapy with doxorubicin is understudied. The objective of this study was to assess the protective effect of mangiferin on liver injury induced by doxorubicin. The doxorubicin was injected intraperitoneally at a total dose of 15 mg/kg b.w, and then the animals were orally supplemented with mangiferin (either 50 and 100 mg/kg b.w) for five weeks. The activity of alanine aminotransferase (ALT), aspartate aminotransferase (AST), superoxide dismutase (SOD), the level of malondialdehyde (MDA) and glutathione, and liver histopathology were examined to evaluate the liver damage. The results showed that doxorubicin could cause liver cell damage to the rats. In addition, the study revealed that the supplementation of mangiferin (50 and 100 mg/kg b.w) decreased ALT and AST activities, as well as lipid peroxidation. It also increased SOD and GSH levels of liver cells (p <0.05). Our study proved that the improvement in liver function and oxidative stress parameters caused by doxorubicin, indicates that the mangiferin has a protective effect against liver damage induced by doxorubicin.
Asian Pacific Journal of Cancer Prevention, 2022
Background: Cervical cancer is the leading cause of cancer death in women. Chemotherapy is one of... more Background: Cervical cancer is the leading cause of cancer death in women. Chemotherapy is one of the treatment modalities with many side effects. This study aimed to evaluate the anticancer activity of soluble ethyl acetate partition of Chromolaena odorata (C. odorata) leaves on HeLa cells of cervical cancer. Material and methods: The cytotoxicity activity of the soluble ethyl acetate extract of the C. odorata leaves was analyzed by MTT colorimetry assay. The apoptosis activity was determined by double staining and flow cytometry techniques. Doubling time assay was used to observed HeLa cells proliferation. Results: The IC 50 of soluble ethyl acetate partition of this plant was 82.41± 6.73 µg/ml against HeLa cells. The apoptosis activity showed that HeLa cells underwent morphological changes in dosedependent manner while the highest number of dead cells was observed after treatment with 500 µg/ml of the partition. Flow cytometry analysis showed that treatment with IC 50 and 2x IC 50 resulted in death of more than 97% cells (p-value <0.05 in both comparisons). Proliferation of HeLa cells was also inhibited following treatment with ½ IC50, IC50, and 2xIC 50 in the first 24 hours (p-value <0.05 in all comparison). Conclusion: The findings of this study suggested that the soluble ethyl acetate partition from ethanol extract of C. odorata leaves had cytotoxic and antiproliferative properties against HeLa cells.
Buku ini membahas malaria dalam kaitannya dengan tantangan dan peluang untuk menemukan dan mengem... more Buku ini membahas malaria dalam kaitannya dengan tantangan dan peluang untuk menemukan dan mengembangkan senyawa baru dari bahan alam sebagai antimalaria, sehingga dapat membantu mengatasi permasalahan resistensi Plasmodium yang semakin meluas. Buku ini untuk pengembangan pengetahuan tentang upaya preventif malaria berbasis bahan alam yang menggambarkan secara molekuler peran Eurikumanon yang terlibat pada pathogenesis pengobatan malaria. Hadirnya buku ini mengajak pembaca akan meningkatkan inovasi dan motivasi untuk mencari pola baru dalam menuntaskan malaria berbasis bahan alam.
Pharmacia, 2021
Background: Breast cancer chemotherapy with standard drugs such as doxorubicin will induce cardio... more Background: Breast cancer chemotherapy with standard drugs such as doxorubicin will induce cardiotoxicity. Therefore, this study aims to evaluate the anticancer activity ofC. odorataleaves extract in DMBA induced breast cancer on rats.Methods: Seven groups ofRattus novergicuswere used: Four treatment groups ofC. odorataextract (500, 1000, 2000, and 4000 mg/kg BW), normal control, breast cancer control, and doxorubicin treatment group. The number, volume, and weight of the nodule and the rats’ body weight were compared among groups. Data was analyzed using paired t-test or one-way ANOVA with post hoc analysis as appropriate.Results: Significant decline of the number, volume, and weight of cancer nodules was observed in the treatment group (p< 0.001). The weight of the cancer nodule at week 16thwas also significantly reduced in GCo2000compared to Gdoxo(p< 0.0001). A significant increase in body weight was also dose-dependent, especially at week 11th(p< 0.05 in all comparisons...
Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition), 2020
Background: Coffee contains caffeine. Caffeine is the main component that influences the response... more Background: Coffee contains caffeine. Caffeine is the main component that influences the response of the cardiovascular system and blood pressure (BP). Acute response of coffee to increased BP is related to caffeine.Objectives: This study aims to analyze the acute response of coffee consumption on BP in healthy females therefore coffee is an alternative therapy for hypotension.Methods: The research design was a clinical trial. Treatment: black coffee, Gayo’s Arabica, 10 mg, coffee brewed with 150 ml of boiling water, without sugar. BP was examined using a mercury sphygmomanometer and stethoscope. BP each subject was examined twice and averaged. BP was checked 3 times: before, 30, and 60 minutes after coffee consumption. All subjects were non-coffee drinkers. A total of 20 healthy female, 18-20 years old were divided into two groups: the non-intervention (n=9) and intervention (n=11). Data was analyzed by independent and paired sample t-test.Results: Coffee increased systolic 10-20 m...
Narra J, 2021
This study was conducted to determine the prevalence of prolonged neuromuscular symptoms, includi... more This study was conducted to determine the prevalence of prolonged neuromuscular symptoms, including fatigue, anosmia, headache, myalgia, and joint pain in COVID-19 survivors hospitalized with mild, moderate, or severe infections worldwide. The search was conducted up to January 30th, 2021 using three databases (PubMed, Scopus, and Web of Science) to identify potentially eligible studies. Data on study characteristics, follow-up characteristics, and severity of COVID-19 during hospitalization were collected in accordance with PRISMA guidelines. The Newcastle-Ottawa scale was used to assess the quality of relevant articles. The estimated prevalence of specific prolonged neuromuscular symptoms and the association between COVID-19 severity and occurrence of prolonged neuromuscular symptoms was analyzed wherever appropriate. Database search yielded 4,050 articles and 22 articles were included for meta-analysis. The estimated prevalence of prolonged fatigue was recorded in 21.2% (95%CI: 1...
Doxorubicin (DOXO) is an anticancer that is often used clinically which induces cardiotoxicity in... more Doxorubicin (DOXO) is an anticancer that is often used clinically which induces cardiotoxicity in the patient. Therefore, finding a new compound that acts as co-chemotherapy is a current challenge. Eurycomanone (EURY) isolated from Eurycoma longifolia Jack have been shown to have anticancer activity in several studies. This study aimed to observe the effect of EURY, DOXO, and its combination on the cell cycle of T47D and MCF-7 breast cancer cell lines. Anticancer activities of EURY and DOXO were examined by MTT colorimetry assay. The effect of combination was analyzed by the Combination Index (CI) method using Compusyn software. The apoptotic activity was analyzed using flow cytometry. To determine CI, a fixed concentration ratio between EURY and DOXO was used from the IC50 value at concentrations (1/2; 1/4; 1/8; 1/16 of IC50). We found that EURY had IC50 values at 0.377μg/mL on T47D and 4.7μg/mL on MCF-7 cells, while DOXO had IC50 values 1.845μg/mL on T47D and 5.074μg/mL on MCF-7ce...
Diabetes poses a public health burden range from the inadequacy of current therapies to complicat... more Diabetes poses a public health burden range from the inadequacy of current therapies to complications. A new diabetic therapy derived from plants with less or no adverse effects is needed. Chromolaena odorata is commonly used as a traditional antidiabetic agent in some countries. The objective of this study was to evaluate the antidiabetic activity of C. odorata leaves extract in diabetic-induced rats. Six groups of Rattus novergicus were used: three concentrations of C. odorata leave extracts (100mg/kg body weight (BW), 200mg/kg BW, and 400mg/kg BW), normal control, diabetic control, and glibenclamide treatment group. C. odorata extract was administrated orally each day for five weeks. The animal body weight, blood glucose level, insulin level, and pathological changes of the pancreas were compared among groups. Data were analyzed using paired t-test or one-way ANOVA with post hoc analysis as appropriate. Our data indicated a significant difference in body weight between the diabet...
Open Access Macedonian Journal of Medical Sciences, 2021
BACKGROUND: Diabetes mellitus (DM) is a chronic and progressive metabolic disease characterized b... more BACKGROUND: Diabetes mellitus (DM) is a chronic and progressive metabolic disease characterized by hyperglycemia due to impaired insulin secretion associated with a lack of phosphoinositide 3-kinase (PI3K) and glucose transporter-4 (GLUT-4) expression in the skeletal muscle membrane. AIM: The aim of the study is to understand the effect of coffee Arabica Gayo leaf extract (Coffea arabica L.) in increasing PI3K expression and GLUT-4 expression in the skeletal muscle membrane. METHODS: Thirty-five male Wistar rats with Type 2 DM (T2DM) induced using a combination of a high-fat diet for 5 weeks followed by multiple intraperitoneal injections of low-dose streptozotocin (30 mg/kg). Divided into seven groups as such two groups that did not receive treatment and five groups that received treatment. The dosage administered was 150, 200, and 250 mg/kg/day through the nasogastric tube for 30 days. PI3K and GLUT-4 expression in the skeletal muscle membrane was evaluated by Immunohistochemistry...