Hano Ali - Academia.edu (original) (raw)

Hano Ali

Phone: 009647711135601
Address: Iraq , Suliamania university

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Research paper thumbnail of Molecular Identification of CandidaSpecies Isolated from Ears of

The goal of this study was to determine internal transcribed spacer/5.8S ribosomal DNA (rDNA) to ... more The goal of this study was to determine internal transcribed spacer/5.8S ribosomal DNA (rDNA) to detect Candida spp. in dogs with Otitis externa. PCR-based detection of internal transcribed spacer 2 (ITS2) regions of
the rRNA genes was evaluated as a means of fungal identification by using Internal transcribed spacers 1 (ITS1) and
Internal transcribed spacers 4 (ITS4). These were amplified by PCR to detect fungal DNA. Sixty swab ear samples
from domestic dogs with and without O. externawere examined for clinical signs including head shaking, scratching
the external ear flaps, bad odor, obstraction of the ear canal, anorexia, emaciation and auricular discharge (unilateral
in 20 and bilateral in 10 dogs). The isolation of Candidaspecies was performed from the external otitic canal in all
animals. Yeast species isolated in 30 cases involving mainly Candida spp.(20 cases) and more often Aspergillus niger(5
cases), Penicellium spp. (3 cases) and Aspergillus fumigatus(2 cases). Twenty isolates of Candidaused to test the selected
primers and conditions of the PCR. Molecular fungal identification is successful in most isolates; with a band size
600-800 bp. This study concluded that size of PCR-amplified ITS2 region DNA is a rapid and reliable method to
identify clinically significant yeasts.

Research paper thumbnail of Molecular Identification of CandidaSpecies Isolated from Ears of

The goal of this study was to determine internal transcribed spacer/5.8S ribosomal DNA (rDNA) to ... more The goal of this study was to determine internal transcribed spacer/5.8S ribosomal DNA (rDNA) to detect Candida spp. in dogs with Otitis externa. PCR-based detection of internal transcribed spacer 2 (ITS2) regions of
the rRNA genes was evaluated as a means of fungal identification by using Internal transcribed spacers 1 (ITS1) and
Internal transcribed spacers 4 (ITS4). These were amplified by PCR to detect fungal DNA. Sixty swab ear samples
from domestic dogs with and without O. externawere examined for clinical signs including head shaking, scratching
the external ear flaps, bad odor, obstraction of the ear canal, anorexia, emaciation and auricular discharge (unilateral
in 20 and bilateral in 10 dogs). The isolation of Candidaspecies was performed from the external otitic canal in all
animals. Yeast species isolated in 30 cases involving mainly Candida spp.(20 cases) and more often Aspergillus niger(5
cases), Penicellium spp. (3 cases) and Aspergillus fumigatus(2 cases). Twenty isolates of Candidaused to test the selected
primers and conditions of the PCR. Molecular fungal identification is successful in most isolates; with a band size
600-800 bp. This study concluded that size of PCR-amplified ITS2 region DNA is a rapid and reliable method to
identify clinically significant yeasts.

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