Harshita Singh - Academia.edu (original) (raw)

Papers by Harshita Singh

Research paper thumbnail of Optimization of medium composition for improving biomass production of Lactobacillus plantarum Pi06 using the Taguchi array design and the Box-Behnken method

Biotechnology and Bioprocess Engineering, 2012

h i g h l i g h t s g r a p h i c a l a b s t r a c t Simultaneous hydrogen and butanol productio... more h i g h l i g h t s g r a p h i c a l a b s t r a c t Simultaneous hydrogen and butanol production from single process. Effective utilization of C5 and C6 sugar for H 2 and Butanol production. Di-ammonium phosphate used as suitable alternative of tryptone as nitrogen source. Energy recovery of 75.22% and 85.61% from xylan and starch achieved, respectively.

Research paper thumbnail of Stress Alleviation in Plants Using SAR and ISR: Current Views on Stress Signaling Network

Microbes and Signaling Biomolecules Against Plant Stress

Research paper thumbnail of A status report on biohydrogen production from algae

Invertis Journal of Science & Technology

Research paper thumbnail of Benign fibrous histiocytoma of the maxilla: A rare case report and literature review

National Journal of Maxillofacial Surgery

Research paper thumbnail of A Study on Role of Micro Finance in Rural Development Ayodhya Region

International Journal for Research in Applied Science and Engineering Technology

India is a country which has its majority of population residing in rural areas hence rural devel... more India is a country which has its majority of population residing in rural areas hence rural development becomes an important aspect on which limelight should be bought. Micro-finance therefore becomes an anti-poverty vaccine. Small funds injected into rural areas will stimulate rural activities which is necessary to boost village economy. Micro-financing empowers bottom of the pyramid group for self-employment opportunities and to lift their standard of living. The study is conducted to examine the current scenario of outreach of micro-finance in said areas and whether it is helping in alleviating poverty. The data collection of the research would be based on primary and secondary data sources.

Research paper thumbnail of Tissue-specific expression pattern of calcium-dependent protein kinases-related kinases (CRKs) in rice

Plant Signaling & Behavior

Research paper thumbnail of Tongue thickness in health vs cirrhosis of the liver: Prospective observational study

World Journal of Gastrointestinal Pharmacology and Therapeutics

Research paper thumbnail of Hot Water Seed Treatment: A Review

Capsicum [Working Title]

In present day agriculture, use of chemicals for crop production is discouraged. Hence, other alt... more In present day agriculture, use of chemicals for crop production is discouraged. Hence, other alternative treatments for disease control must be developed, and hot water treatment is one of them. It is a feasible practice, both financially and time wise. Hot water soaking is a very age-old practice, efficient in destroying pathogens borne both outside the testa and inside the seed testa by using temperature hot enough to kill the organism but not quite hot enough to kill the seed. Extensive research work has been reported on hot water treatment in vegetables. Therefore, an attempt has been made to review the information available regarding the effect of hot water treatment on growth, disease incidence and yield of vegetables.

Research paper thumbnail of Volume change following valsalva: A Valuable tool to be incorporated in the stepwise technique for central venous catheter placement under ultrasound guidance: An opinion

Annals of Cardiac Anaesthesia

Research paper thumbnail of Process optimization for enhanced biodiesel production by Neochloris oleoabundans UTEX 1185 with concomitant CO2 sequestration

Industrial & Engineering Chemistry Research

Research paper thumbnail of Isolation, Screening, and Identification of Novel Isolates of Actinomycetes from India for Antimicrobial Applications

Frontiers in Microbiology, 2016

The search for novel bioactive compounds from the natural environment has rapidly been gaining mo... more The search for novel bioactive compounds from the natural environment has rapidly been gaining momentum with the increase in multi-drug resistant (MDR) pathogens. In the present study, the antimicrobial potential of novel actinomycetes has been evaluated by initial screening of six soil samples. Primary and secondary screening was performed against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Candida albicans, Candida tropicalis, Trichophyton rubrum, and other MDR bacterial and fungal test strains, thirteen active isolates were selected for further study. Microbial strains were identified on the basis of growth conditions and other biochemical characters. Five most active microbial strains were identified using 16S rRNA sequence homology and designated as Streptomyces xanthophaeus MTCC 11938, Streptomyces variabilis MTCC 12266, Streptomyces xanthochromogenes MTCC 11937, Streptomyces levis EU 124569, and Streptomyces sp. NCIM 5500. Four antibacterial and three antifungal compounds isolated from the above five isolates were purified and partially characterized using UV absorption and IR spectra. Two antibacterial metabolites, belong to chromone and peptide antibiotic, respectively. The antifungal compounds were found to be of non-polyene nature. In conclusion, we study the isolation of novel bacterial strains of actinomycetes for producing novel compounds having antibacterial and antifungal activities from the unexplored agro-ecological niches of India. Also, this study paves the way for further characterization of these isolates of Streptomyces sp. for their optimum utilization for antimicrobial purposes.

Research paper thumbnail of Changing Pattern of Employee Relations in India - a Review of Relevant Academic Literature and Methodologies

Review of Professional Management- A Journal of New Delhi Institute of Management, 2016

Research paper thumbnail of Industrial Unrest at Tesco Cranes

Research paper thumbnail of Effect of cervical mobilization and ischemic compression therapy on contralateral cervical side flexion and pressure pain threshold in latent upper trapezius trigger points

Journal of Bodywork and Movement Therapies, 2015

Studies have shown a clinical relationship between trigger points and joint impairments. However ... more Studies have shown a clinical relationship between trigger points and joint impairments. However the cause-and effect relationship between muscle and joint dysfunctions in trigger points could not be established. The purpose of this study was to investigate effects of mobilization and ischemic compression therapy on cervical range of motion and pressure pain sensitivity in participants with latent trigger point in the upper trapezius muscle. Ninety asymptomatic participants with upper trapezius latent trigger point were randomized in to 3 groups: mobilization, ischemic compression and a control. The outcomes were measured over a 2 week period. Repeated measures ANOVA showed statistically and clinically significant pre to post improvement in both the interventional groups compared to control (p < 0.05). However the effect sizes between the intervention groups were small (<0.3) revealing minimal clinical detectable difference.

Research paper thumbnail of Conformational analysis and vibrational study of daidzein by using FT-IR and FT-Raman spectroscopies and DFT calculations

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2014

Daidzein (C15H10O4) is a type of isoflavone. It was isolated from Butea monosperma that belongs t... more Daidzein (C15H10O4) is a type of isoflavone. It was isolated from Butea monosperma that belongs to the Fabaceae family. Soybeans and soy products are the abundant source of daidzein. It is the subject of investigation for many reasons, as it has got wide applications, such as anti-tumor, anti-estrogen, weak pro-estrogen and anti-cancer activities. In the present study, a complete vibrational assignment is provided for the observed IR and Raman spectra of daidzein. Electronic properties have been analyzed using TD-DFT method for both gaseous and solvent phase. The optimized geometry, total energy, potential energy surface and vibrational wavenumbers of daidzein have been determined using density functional theory (DFT/B3LYP) method with 6-311++G(d,p) basis set and a good correlation was found between observed and calculated values. The double well potential energy curve of the molecule about three bonds, has been plotted, as obtained from DFT/6-31G basis. The HOMO-LUMO energy gap of possible conformers has been calculated for comparing their chemical activity. Global reactivity descriptors have been calculated for predicting the chemical reactivity and the stability of chemical systems. Electrostatic potential surface has been plotted for predicting the structure activity relationship. NBO analysis has also been performed to study the stability of the molecule. NLO study reveals the nonlinear properties of the molecule. 1H and 13C NMR spectra have also been studied. Finally, the calculated results were used to simulate infrared and Raman spectra of the title compound which showed a good agreement with the observed spectra.

Research paper thumbnail of Optimization of medium composition for improving biomass production of Lactobacillus plantarum Pi06 using the Taguchi array design and the Box-Behnken method

Biotechnology and Bioprocess Engineering, 2012

h i g h l i g h t s g r a p h i c a l a b s t r a c t Simultaneous hydrogen and butanol productio... more h i g h l i g h t s g r a p h i c a l a b s t r a c t Simultaneous hydrogen and butanol production from single process. Effective utilization of C5 and C6 sugar for H 2 and Butanol production. Di-ammonium phosphate used as suitable alternative of tryptone as nitrogen source. Energy recovery of 75.22% and 85.61% from xylan and starch achieved, respectively.

Research paper thumbnail of Stress Alleviation in Plants Using SAR and ISR: Current Views on Stress Signaling Network

Microbes and Signaling Biomolecules Against Plant Stress

Research paper thumbnail of A status report on biohydrogen production from algae

Invertis Journal of Science & Technology

Research paper thumbnail of Benign fibrous histiocytoma of the maxilla: A rare case report and literature review

National Journal of Maxillofacial Surgery

Research paper thumbnail of A Study on Role of Micro Finance in Rural Development Ayodhya Region

International Journal for Research in Applied Science and Engineering Technology

India is a country which has its majority of population residing in rural areas hence rural devel... more India is a country which has its majority of population residing in rural areas hence rural development becomes an important aspect on which limelight should be bought. Micro-finance therefore becomes an anti-poverty vaccine. Small funds injected into rural areas will stimulate rural activities which is necessary to boost village economy. Micro-financing empowers bottom of the pyramid group for self-employment opportunities and to lift their standard of living. The study is conducted to examine the current scenario of outreach of micro-finance in said areas and whether it is helping in alleviating poverty. The data collection of the research would be based on primary and secondary data sources.

Research paper thumbnail of Tissue-specific expression pattern of calcium-dependent protein kinases-related kinases (CRKs) in rice

Plant Signaling & Behavior

Research paper thumbnail of Tongue thickness in health vs cirrhosis of the liver: Prospective observational study

World Journal of Gastrointestinal Pharmacology and Therapeutics

Research paper thumbnail of Hot Water Seed Treatment: A Review

Capsicum [Working Title]

In present day agriculture, use of chemicals for crop production is discouraged. Hence, other alt... more In present day agriculture, use of chemicals for crop production is discouraged. Hence, other alternative treatments for disease control must be developed, and hot water treatment is one of them. It is a feasible practice, both financially and time wise. Hot water soaking is a very age-old practice, efficient in destroying pathogens borne both outside the testa and inside the seed testa by using temperature hot enough to kill the organism but not quite hot enough to kill the seed. Extensive research work has been reported on hot water treatment in vegetables. Therefore, an attempt has been made to review the information available regarding the effect of hot water treatment on growth, disease incidence and yield of vegetables.

Research paper thumbnail of Volume change following valsalva: A Valuable tool to be incorporated in the stepwise technique for central venous catheter placement under ultrasound guidance: An opinion

Annals of Cardiac Anaesthesia

Research paper thumbnail of Process optimization for enhanced biodiesel production by Neochloris oleoabundans UTEX 1185 with concomitant CO2 sequestration

Industrial & Engineering Chemistry Research

Research paper thumbnail of Isolation, Screening, and Identification of Novel Isolates of Actinomycetes from India for Antimicrobial Applications

Frontiers in Microbiology, 2016

The search for novel bioactive compounds from the natural environment has rapidly been gaining mo... more The search for novel bioactive compounds from the natural environment has rapidly been gaining momentum with the increase in multi-drug resistant (MDR) pathogens. In the present study, the antimicrobial potential of novel actinomycetes has been evaluated by initial screening of six soil samples. Primary and secondary screening was performed against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Candida albicans, Candida tropicalis, Trichophyton rubrum, and other MDR bacterial and fungal test strains, thirteen active isolates were selected for further study. Microbial strains were identified on the basis of growth conditions and other biochemical characters. Five most active microbial strains were identified using 16S rRNA sequence homology and designated as Streptomyces xanthophaeus MTCC 11938, Streptomyces variabilis MTCC 12266, Streptomyces xanthochromogenes MTCC 11937, Streptomyces levis EU 124569, and Streptomyces sp. NCIM 5500. Four antibacterial and three antifungal compounds isolated from the above five isolates were purified and partially characterized using UV absorption and IR spectra. Two antibacterial metabolites, belong to chromone and peptide antibiotic, respectively. The antifungal compounds were found to be of non-polyene nature. In conclusion, we study the isolation of novel bacterial strains of actinomycetes for producing novel compounds having antibacterial and antifungal activities from the unexplored agro-ecological niches of India. Also, this study paves the way for further characterization of these isolates of Streptomyces sp. for their optimum utilization for antimicrobial purposes.

Research paper thumbnail of Changing Pattern of Employee Relations in India - a Review of Relevant Academic Literature and Methodologies

Review of Professional Management- A Journal of New Delhi Institute of Management, 2016

Research paper thumbnail of Industrial Unrest at Tesco Cranes

Research paper thumbnail of Effect of cervical mobilization and ischemic compression therapy on contralateral cervical side flexion and pressure pain threshold in latent upper trapezius trigger points

Journal of Bodywork and Movement Therapies, 2015

Studies have shown a clinical relationship between trigger points and joint impairments. However ... more Studies have shown a clinical relationship between trigger points and joint impairments. However the cause-and effect relationship between muscle and joint dysfunctions in trigger points could not be established. The purpose of this study was to investigate effects of mobilization and ischemic compression therapy on cervical range of motion and pressure pain sensitivity in participants with latent trigger point in the upper trapezius muscle. Ninety asymptomatic participants with upper trapezius latent trigger point were randomized in to 3 groups: mobilization, ischemic compression and a control. The outcomes were measured over a 2 week period. Repeated measures ANOVA showed statistically and clinically significant pre to post improvement in both the interventional groups compared to control (p < 0.05). However the effect sizes between the intervention groups were small (<0.3) revealing minimal clinical detectable difference.

Research paper thumbnail of Conformational analysis and vibrational study of daidzein by using FT-IR and FT-Raman spectroscopies and DFT calculations

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2014

Daidzein (C15H10O4) is a type of isoflavone. It was isolated from Butea monosperma that belongs t... more Daidzein (C15H10O4) is a type of isoflavone. It was isolated from Butea monosperma that belongs to the Fabaceae family. Soybeans and soy products are the abundant source of daidzein. It is the subject of investigation for many reasons, as it has got wide applications, such as anti-tumor, anti-estrogen, weak pro-estrogen and anti-cancer activities. In the present study, a complete vibrational assignment is provided for the observed IR and Raman spectra of daidzein. Electronic properties have been analyzed using TD-DFT method for both gaseous and solvent phase. The optimized geometry, total energy, potential energy surface and vibrational wavenumbers of daidzein have been determined using density functional theory (DFT/B3LYP) method with 6-311++G(d,p) basis set and a good correlation was found between observed and calculated values. The double well potential energy curve of the molecule about three bonds, has been plotted, as obtained from DFT/6-31G basis. The HOMO-LUMO energy gap of possible conformers has been calculated for comparing their chemical activity. Global reactivity descriptors have been calculated for predicting the chemical reactivity and the stability of chemical systems. Electrostatic potential surface has been plotted for predicting the structure activity relationship. NBO analysis has also been performed to study the stability of the molecule. NLO study reveals the nonlinear properties of the molecule. 1H and 13C NMR spectra have also been studied. Finally, the calculated results were used to simulate infrared and Raman spectra of the title compound which showed a good agreement with the observed spectra.