Hasan Bilgili - Academia.edu (original) (raw)

Papers by Hasan Bilgili

Research paper thumbnail of Thermal necrosis induced by electrocauterization as a local adjuvant therapy in local aggressive bone tumors, what is the safe limit for surgical margins? An experimental study

Archives of Orthopaedic and Trauma Surgery, 2015

In the current study, it was aimed to investigate the temperature change in the cavity wall and p... more In the current study, it was aimed to investigate the temperature change in the cavity wall and pathologic necrosis occurred during cauterization, which was applied at different voltages and time intervals. The right tibias of 32 male rabbits were used. Three 2-mm-diameter holes were created on the cortical surface of the tibia using a hand-held drill. Using an electrocautery device, 55 mV was applied for 3 and 5 s and 65 mV was applied for 3 and 5 s. Maximum temperatures at 3 and 6 mm distance from the application site were measured. Biopsy specimens obtained at 3 and 6 mm distance from the application site were evaluated microscopically for bone cell viability and periosteal necrosis. Thirty-two rabbits were divided into four groups. In all groups, periosteal bone cells located at the region, extending from the application site to 3 mm distance, died. In this region, application of 55 mV for 3 s caused peripheral necrosis. There were significant differences between the four groups in terms of maximum temperatures measured at 3 mm distance from the application site (p = 0.027). On the other hand, no significant differences were noted between the four groups in terms of maximum temperatures measured at 6 mm distance from the application site (p > 0.05). Cauterization of the cavity wall in the spray mode at 55 mV for 3 s after tumor resection caused necrosis in the cavity wall, extending from the application site to 3 mm distance. Experimental animal study, Level II.

Research paper thumbnail of Bilateral carpal hyperextension cases in dogs and operative treatment methods

Turkish Journal of Veterinary and Animal Sciences, Jan 15, 1999

Research paper thumbnail of Bir Köpekte Bilateral Carpal Hyperextension Olgusunun Carpal Eklem'İn Panarthrodesis'İ Yöntemi İle Sağaltimi

Ankara Üniversitesi Veteriner Fakültesi Dergisi, 1995

Research paper thumbnail of BjR KÖPEKTE BjLATERAL CARPALHYPEREXTENS/ON OLGUSUNUN CARPAL EKLEM'jN PANARTHRODESjS'j YÖNTEMj JLE SAGALT/M

Treatment of a bilateral carpal hyperextension case in a dog, by panarthrodesis method of carpal ... more Treatment of a bilateral carpal hyperextension case in a dog, by panarthrodesis method of carpal joint.

Research paper thumbnail of Thermal necrosis induced by electrocauterization as a local adjuvant therapy in local aggressive bone tumors, what is the safe limit for surgical margins? An experimental study

Archives of Orthopaedic and Trauma Surgery, 2015

In the current study, it was aimed to investigate the temperature change in the cavity wall and p... more In the current study, it was aimed to investigate the temperature change in the cavity wall and pathologic necrosis occurred during cauterization, which was applied at different voltages and time intervals. The right tibias of 32 male rabbits were used. Three 2-mm-diameter holes were created on the cortical surface of the tibia using a hand-held drill. Using an electrocautery device, 55 mV was applied for 3 and 5 s and 65 mV was applied for 3 and 5 s. Maximum temperatures at 3 and 6 mm distance from the application site were measured. Biopsy specimens obtained at 3 and 6 mm distance from the application site were evaluated microscopically for bone cell viability and periosteal necrosis. Thirty-two rabbits were divided into four groups. In all groups, periosteal bone cells located at the region, extending from the application site to 3 mm distance, died. In this region, application of 55 mV for 3 s caused peripheral necrosis. There were significant differences between the four groups in terms of maximum temperatures measured at 3 mm distance from the application site (p = 0.027). On the other hand, no significant differences were noted between the four groups in terms of maximum temperatures measured at 6 mm distance from the application site (p > 0.05). Cauterization of the cavity wall in the spray mode at 55 mV for 3 s after tumor resection caused necrosis in the cavity wall, extending from the application site to 3 mm distance. Experimental animal study, Level II.

Research paper thumbnail of Buzağilarda Uzun Kemik Kiriklarinin Ilizarov Tekniği İle Sağaltim Olanaklarinin Araştirilmasi

Ankara Üniversitesi Veteriner Fakültesi Dergisi, 1999

Research paper thumbnail of Bir Kedide II. Tip Monteggia Lezyonu Ve Operatif Sağaltimi

Ankara Üniversitesi Veteriner Fakültesi Dergisi, 1997

Research paper thumbnail of The effect of extracorporeal shock wave treatment (ESWT) on bone defects. An experimental study

Bulletin (Hospital for Joint Diseases (New York, N.Y.)), 1999

The aim of this study was to investigate the effects of extracorporeal shock wave therapy (ESWT) ... more The aim of this study was to investigate the effects of extracorporeal shock wave therapy (ESWT) on the formation of callus in bone defects created in rabbit radii. this study searches for an answer to whether ESWT may have a therapeutic effect on bone defects. A bone defect with a radius of 1 cm was created in both forelimbs of 20 rabbits. At the 7th, 14th, and 21st days ESWT treatment was applied to the forming callus in the right radius under fluoroscopic control. At the 6th and 12th weeks, the animals were sacrificed and callus analysis was performed by computerized scan, dual energy x-ray absorptiometer. Histological analyses were also performed. The results revealed that the average callus area in the right (ESWT applied) radial defect was greater in both groups and statistically significant at the 12th week (p < 0.05). There was no difference in bone density between defects. Histologically the callus area was greater on the right side (ESWT applied side) in both groups. Ho...

Research paper thumbnail of Ortopedik Problemli 15 Köpek ve 7 Kedinin Manuflex® Eksternal Fikzatör İle Sağaltımı

Kafkas Universitesi Veteriner Fakultesi Dergisi, 2014

Research paper thumbnail of Treatment Techniques of Femoral Quadriceps Muscle Contracture in Ten Dogs and Two Cats

Kafkas Universitesi Veteriner Fakultesi Dergisi, 2009

This study was performed on 10 dogs and 2 cats, were shown quadriceps contracture (QC). The etiol... more This study was performed on 10 dogs and 2 cats, were shown quadriceps contracture (QC). The etiological history was indicated that QC occurred as a result of improper bandage application or operations due to femoral fractures in 11 cases or congenital in one case. Long-term ...

Research paper thumbnail of In vitro/in vivo evaluation of the efficiency of teicoplanin-loaded biodegradable microparticles formulated for implantation to infected bone defects

Journal of Microencapsulation, 2003

Research paper thumbnail of Correction of saddle nose deformities by coral implantation

European Archives of Oto-Rhino-Laryngology, 1997

The coral or Madrepororia is a sea invertebrate with a skeleton morphologically and chemically ve... more The coral or Madrepororia is a sea invertebrate with a skeleton morphologically and chemically very similar to mineral bone. In 1990 we implanted coral blocks, sterilized by gamma irradiation, into the nasal dorsa of ten dogs. Resorption and substitution with bone or fibrous tissue were observed within 6 months. Between 1991 and 1994 biocoral was used in 20 patients with saddle nose deformities in order to augment the nasal pyramid and dome. Two extrusions were observed in the early postoperative period during follow-up. One of these extrusions was related to trauma and necessitated removal of the implant. Our findings show that coral is a suitable alloplastic material that can be used in the correction of saddle nose deformities.

Research paper thumbnail of Successful Treatment of Insulin Allergy in a 1-Year-Old Infant With Neonatal Diabetes by Lispro and Glargine Insulin

Diabetes Care, 2005

A neonate girl, the first child of consanguineous parents, was born at full term after uneventful... more A neonate girl, the first child of consanguineous parents, was born at full term after uneventful pregnancy. She was small for gestational age with a birth weight of 1,430 g. Although the infant had been fed properly from birth, she was presented with complaint of poor weight gain at ...

Research paper thumbnail of Chitosan and alginate scaffolds for bone tissue regeneration

Pharmazie, 2007

... Three dimensional and porous structure of gels or sponges can provide a scaffold for bone cel... more ... Three dimensional and porous structure of gels or sponges can provide a scaffold for bone cells to grow in and expansion of cells regularly to form the new bone tissue and its biodegrad-ability allow the drugs to be released to implantation sites (Drury and Mooney, 2003; Vats ...

Research paper thumbnail of Use of a circular external skeletal fixator to treat comminuted metacarpal and tibial fractures in six calves

The Veterinary Record, Jun 12, 2008

A circular external skeletal fixator system was used to fix long-bone fractures in six calves, th... more A circular external skeletal fixator system was used to fix long-bone fractures in six calves, three with tibial fractures and three with metacarpal fractures. An Ilizarov apparatus of appropriate diameter, depending on the calves&#39; bodyweight, was used. In the postoperative period, clinical and radiological examinations were carried out to assess fracture healing, the stability of the fixator and when the calves could make full use of the limb. The fixator was tolerated well, the fractures healed and the fixators maintained rigid stability. The calves were able to use their limbs fully after between 15 and 27 days.

Research paper thumbnail of Effect of intraoperative PEEP application on colonic anastomoses healing: An experimental animal study

International Surgery, 2015

This study aimed to assess the effect of intraoperative PEEP intervention on the healing of colon... more This study aimed to assess the effect of intraoperative PEEP intervention on the healing of colonic anastomoses in rabbits. Thirty-two New Zealand type male rabbits were divided into two groups of sixteen animals each. Following ventilation with tracheostomy, colonic resection and anastomosis were performed in both groups. While 10 cm H2O PEEP level was applied in Group I (PEEP), Group II (ZEEP) was ventilated without PEEP throughout the surgery. Half of the both PEEP and ZEEP group animals were killed on the third postoperative day, while the remaining half on the seventh. Anastomotic bursting pressures, the tissue concentrations in hydroxyproline, and histological assessments were performed. Besides, intraoperative oxygen saturation and postoperative arterial blood gas parameters were also compared. On the first postoperative day, both arterial oxygen tension (PO2) and oxygen saturation (SO2) in the PEEP group were significantly higher than in the ZEEP group. On the seventh postoperative day, the bursting pressures of the anastomoses were significantly higher in the PEEP group, however the hydroxyproline content was significantly lower in the PEEP group than that in the ZEEP group. At day 7, PEEP group was significantly associated with increased neoangiogenesis compared with the ZEEP group. The anastomotic healing process is positively influenced by the intraoperative PEEP application.

Research paper thumbnail of Use of a circular external skeletal fixator to treat comminuted metacarpal and tibial fractures in six calves

The Veterinary record, Jan 6, 2008

A circular external skeletal fixator system was used to fix long-bone fractures in six calves, th... more A circular external skeletal fixator system was used to fix long-bone fractures in six calves, three with tibial fractures and three with metacarpal fractures. An Ilizarov apparatus of appropriate diameter, depending on the calves' bodyweight, was used. In the postoperative period, clinical and radiological examinations were carried out to assess fracture healing, the stability of the fixator and when the calves could make full use of the limb. The fixator was tolerated well, the fractures healed and the fixators maintained rigid stability. The calves were able to use their limbs fully after between 15 and 27 days.

Research paper thumbnail of Effects of Vascular Bundle Implantation on Autograft, Fresh-Frozen Allograft, and Xenograft Incorporation in a Rabbit Model

Orthopedics, 2008

This study examined the effects of vascular bundle implantation into a bone graft. Vascularized a... more This study examined the effects of vascular bundle implantation into a bone graft. Vascularized and nonvascularized autografts, allografts, and xenografts were placed inside defects in the proximal tibia in rabbits. Evaluation using radiographs, magnetic resonance imaging, bone scintigraphy, and microscopy showed autografts fused more rapidly than allografts and xenografts, and the majority of the vascularized grafts were incorporated completely. Autografts emerged as the gold standard. These findings indicate vessel implantation enhances and accelerates vascularization, new bone formation, and incorporation in autografts, allografts, and xenografts.

Research paper thumbnail of Scintigraphic imaging of radiolabelled drug delivery systems in rabbits with arthritis

International Journal of Pharmaceutics, 2005

This paper describes a novel approach for designing drug delivery systems for intra-articular (i.... more This paper describes a novel approach for designing drug delivery systems for intra-articular (i.a.) treatment of rheumatoid arthritis. Retention of these systems was evaluated by radiolabeling with Tc-99m and gamma scintigraphy in arthritic rabbits. Liposome, niosome, lipogelosome and niogelosome formulations of Diclofenac Sodium (DFNa) have been prepared and drug release properties and in vitro characterisation studies have been carried out. According to characterisation results L1 (DMPC: CHOL: DCP (7:1:2)), L1J1 (DMPC: CHOL: DCP (7:1:2) in C-940 1:1 (w/w)), N (SUR I: CHOL: DCP (7:1:2)) and NJ1 (SUR I: CHOL: DCP (7:1:2) in C-940 1:1 (w/w)) formulations were chosen for the further studies. Retention time of these formulations was evaluated by gamma scintigraphic imaging studies. Rabbits with antigen-induced arthritis were injected intra-articularly with Tc-99m labelled drug delivery systems. Serial scintigraphic images were obtained to investigate the retentions of labelled drug delivery systems in the arthritic joints and choose a suitable formulation for the treatment protocol of arthritis.

Research paper thumbnail of The effect of gamma radiation sterilization on dental biomaterials

Tissue Engineering and Regenerative Medicine, 2014

Biomaterials are used in the field of bone and tissue engineering, orthopaedics and dentistry. De... more Biomaterials are used in the field of bone and tissue engineering, orthopaedics and dentistry. Dental biomaterials including commercially available biodegradable materials act as physical barriers to help quicker healing while stimulating the regeneration of periodontal tissues, which is defined as Guided Tissue Regeneration (GTR). Amongst natural and synthetic biomaterials, collagen and aliphatic polyesters, such as polylactic acid (PLA) and poly (lactic-co-glycolic) acid (PLGA) are the most frequently used biomaterials for regenerative therapies due to their excellent biocompatibility and biodegradability. Due to their resorption in the body and interaction with biological systems, the GTR membranes must be sterile and pyrogen free. The sterility and apyrogenicity of the GTR membranes before human use is a regulatory requirement, however the sterilization of biomaterials is challenging due to the physicochemical changes and toxic residues with the commonly used sterilization techniques. The purpose of the present study was to evaluate the effect of gamma radiation and ethylene oxide sterilization on dental biomaterials with analytical, microbiological and histological examinations. PLGA-based GTR dental biomaterial is selected as the most gamma stable membrane according to the FTIR, DSC, TGA, and SEM results. This dental membrane was sterilized with ethylene oxide (EtO) and the effect of sterilization method on PLGA-based membrane was also investigated. Animal experiments were carried out to evaluate the regenerative properties and inflammatory responses of gamma and EtO sterilized PLGA-based GTR membrane after implantation. Histological examinations showed that resorption and bone formation of gamma sterilized PLGA-based GTR membrane was completed in 12 weeks without any inflammatory response; while only 60.095 ± 2.019% of new bone formation was observed with EtO sterilized one. Gamma sterilized PLGA membrane had significantly faster (P < 0.05) resorption and bone formation in comparison with EtO sterilization. In conclusion, the PLGA-based biomaterials could be sterilized safely and time-and cost-effectively with validated radiation doses for the tissue engineering applications.

Research paper thumbnail of Thermal necrosis induced by electrocauterization as a local adjuvant therapy in local aggressive bone tumors, what is the safe limit for surgical margins? An experimental study

Archives of Orthopaedic and Trauma Surgery, 2015

In the current study, it was aimed to investigate the temperature change in the cavity wall and p... more In the current study, it was aimed to investigate the temperature change in the cavity wall and pathologic necrosis occurred during cauterization, which was applied at different voltages and time intervals. The right tibias of 32 male rabbits were used. Three 2-mm-diameter holes were created on the cortical surface of the tibia using a hand-held drill. Using an electrocautery device, 55 mV was applied for 3 and 5 s and 65 mV was applied for 3 and 5 s. Maximum temperatures at 3 and 6 mm distance from the application site were measured. Biopsy specimens obtained at 3 and 6 mm distance from the application site were evaluated microscopically for bone cell viability and periosteal necrosis. Thirty-two rabbits were divided into four groups. In all groups, periosteal bone cells located at the region, extending from the application site to 3 mm distance, died. In this region, application of 55 mV for 3 s caused peripheral necrosis. There were significant differences between the four groups in terms of maximum temperatures measured at 3 mm distance from the application site (p = 0.027). On the other hand, no significant differences were noted between the four groups in terms of maximum temperatures measured at 6 mm distance from the application site (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt; 0.05). Cauterization of the cavity wall in the spray mode at 55 mV for 3 s after tumor resection caused necrosis in the cavity wall, extending from the application site to 3 mm distance. Experimental animal study, Level II.

Research paper thumbnail of Bilateral carpal hyperextension cases in dogs and operative treatment methods

Turkish Journal of Veterinary and Animal Sciences, Jan 15, 1999

Research paper thumbnail of Bir Köpekte Bilateral Carpal Hyperextension Olgusunun Carpal Eklem'İn Panarthrodesis'İ Yöntemi İle Sağaltimi

Ankara Üniversitesi Veteriner Fakültesi Dergisi, 1995

Research paper thumbnail of BjR KÖPEKTE BjLATERAL CARPALHYPEREXTENS/ON OLGUSUNUN CARPAL EKLEM'jN PANARTHRODESjS'j YÖNTEMj JLE SAGALT/M

Treatment of a bilateral carpal hyperextension case in a dog, by panarthrodesis method of carpal ... more Treatment of a bilateral carpal hyperextension case in a dog, by panarthrodesis method of carpal joint.

Research paper thumbnail of Thermal necrosis induced by electrocauterization as a local adjuvant therapy in local aggressive bone tumors, what is the safe limit for surgical margins? An experimental study

Archives of Orthopaedic and Trauma Surgery, 2015

In the current study, it was aimed to investigate the temperature change in the cavity wall and p... more In the current study, it was aimed to investigate the temperature change in the cavity wall and pathologic necrosis occurred during cauterization, which was applied at different voltages and time intervals. The right tibias of 32 male rabbits were used. Three 2-mm-diameter holes were created on the cortical surface of the tibia using a hand-held drill. Using an electrocautery device, 55 mV was applied for 3 and 5 s and 65 mV was applied for 3 and 5 s. Maximum temperatures at 3 and 6 mm distance from the application site were measured. Biopsy specimens obtained at 3 and 6 mm distance from the application site were evaluated microscopically for bone cell viability and periosteal necrosis. Thirty-two rabbits were divided into four groups. In all groups, periosteal bone cells located at the region, extending from the application site to 3 mm distance, died. In this region, application of 55 mV for 3 s caused peripheral necrosis. There were significant differences between the four groups in terms of maximum temperatures measured at 3 mm distance from the application site (p = 0.027). On the other hand, no significant differences were noted between the four groups in terms of maximum temperatures measured at 6 mm distance from the application site (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt; 0.05). Cauterization of the cavity wall in the spray mode at 55 mV for 3 s after tumor resection caused necrosis in the cavity wall, extending from the application site to 3 mm distance. Experimental animal study, Level II.

Research paper thumbnail of Buzağilarda Uzun Kemik Kiriklarinin Ilizarov Tekniği İle Sağaltim Olanaklarinin Araştirilmasi

Ankara Üniversitesi Veteriner Fakültesi Dergisi, 1999

Research paper thumbnail of Bir Kedide II. Tip Monteggia Lezyonu Ve Operatif Sağaltimi

Ankara Üniversitesi Veteriner Fakültesi Dergisi, 1997

Research paper thumbnail of The effect of extracorporeal shock wave treatment (ESWT) on bone defects. An experimental study

Bulletin (Hospital for Joint Diseases (New York, N.Y.)), 1999

The aim of this study was to investigate the effects of extracorporeal shock wave therapy (ESWT) ... more The aim of this study was to investigate the effects of extracorporeal shock wave therapy (ESWT) on the formation of callus in bone defects created in rabbit radii. this study searches for an answer to whether ESWT may have a therapeutic effect on bone defects. A bone defect with a radius of 1 cm was created in both forelimbs of 20 rabbits. At the 7th, 14th, and 21st days ESWT treatment was applied to the forming callus in the right radius under fluoroscopic control. At the 6th and 12th weeks, the animals were sacrificed and callus analysis was performed by computerized scan, dual energy x-ray absorptiometer. Histological analyses were also performed. The results revealed that the average callus area in the right (ESWT applied) radial defect was greater in both groups and statistically significant at the 12th week (p < 0.05). There was no difference in bone density between defects. Histologically the callus area was greater on the right side (ESWT applied side) in both groups. Ho...

Research paper thumbnail of Ortopedik Problemli 15 Köpek ve 7 Kedinin Manuflex® Eksternal Fikzatör İle Sağaltımı

Kafkas Universitesi Veteriner Fakultesi Dergisi, 2014

Research paper thumbnail of Treatment Techniques of Femoral Quadriceps Muscle Contracture in Ten Dogs and Two Cats

Kafkas Universitesi Veteriner Fakultesi Dergisi, 2009

This study was performed on 10 dogs and 2 cats, were shown quadriceps contracture (QC). The etiol... more This study was performed on 10 dogs and 2 cats, were shown quadriceps contracture (QC). The etiological history was indicated that QC occurred as a result of improper bandage application or operations due to femoral fractures in 11 cases or congenital in one case. Long-term ...

Research paper thumbnail of In vitro/in vivo evaluation of the efficiency of teicoplanin-loaded biodegradable microparticles formulated for implantation to infected bone defects

Journal of Microencapsulation, 2003

Research paper thumbnail of Correction of saddle nose deformities by coral implantation

European Archives of Oto-Rhino-Laryngology, 1997

The coral or Madrepororia is a sea invertebrate with a skeleton morphologically and chemically ve... more The coral or Madrepororia is a sea invertebrate with a skeleton morphologically and chemically very similar to mineral bone. In 1990 we implanted coral blocks, sterilized by gamma irradiation, into the nasal dorsa of ten dogs. Resorption and substitution with bone or fibrous tissue were observed within 6 months. Between 1991 and 1994 biocoral was used in 20 patients with saddle nose deformities in order to augment the nasal pyramid and dome. Two extrusions were observed in the early postoperative period during follow-up. One of these extrusions was related to trauma and necessitated removal of the implant. Our findings show that coral is a suitable alloplastic material that can be used in the correction of saddle nose deformities.

Research paper thumbnail of Successful Treatment of Insulin Allergy in a 1-Year-Old Infant With Neonatal Diabetes by Lispro and Glargine Insulin

Diabetes Care, 2005

A neonate girl, the first child of consanguineous parents, was born at full term after uneventful... more A neonate girl, the first child of consanguineous parents, was born at full term after uneventful pregnancy. She was small for gestational age with a birth weight of 1,430 g. Although the infant had been fed properly from birth, she was presented with complaint of poor weight gain at ...

Research paper thumbnail of Chitosan and alginate scaffolds for bone tissue regeneration

Pharmazie, 2007

... Three dimensional and porous structure of gels or sponges can provide a scaffold for bone cel... more ... Three dimensional and porous structure of gels or sponges can provide a scaffold for bone cells to grow in and expansion of cells regularly to form the new bone tissue and its biodegrad-ability allow the drugs to be released to implantation sites (Drury and Mooney, 2003; Vats ...

Research paper thumbnail of Use of a circular external skeletal fixator to treat comminuted metacarpal and tibial fractures in six calves

The Veterinary Record, Jun 12, 2008

A circular external skeletal fixator system was used to fix long-bone fractures in six calves, th... more A circular external skeletal fixator system was used to fix long-bone fractures in six calves, three with tibial fractures and three with metacarpal fractures. An Ilizarov apparatus of appropriate diameter, depending on the calves&#39; bodyweight, was used. In the postoperative period, clinical and radiological examinations were carried out to assess fracture healing, the stability of the fixator and when the calves could make full use of the limb. The fixator was tolerated well, the fractures healed and the fixators maintained rigid stability. The calves were able to use their limbs fully after between 15 and 27 days.

Research paper thumbnail of Effect of intraoperative PEEP application on colonic anastomoses healing: An experimental animal study

International Surgery, 2015

This study aimed to assess the effect of intraoperative PEEP intervention on the healing of colon... more This study aimed to assess the effect of intraoperative PEEP intervention on the healing of colonic anastomoses in rabbits. Thirty-two New Zealand type male rabbits were divided into two groups of sixteen animals each. Following ventilation with tracheostomy, colonic resection and anastomosis were performed in both groups. While 10 cm H2O PEEP level was applied in Group I (PEEP), Group II (ZEEP) was ventilated without PEEP throughout the surgery. Half of the both PEEP and ZEEP group animals were killed on the third postoperative day, while the remaining half on the seventh. Anastomotic bursting pressures, the tissue concentrations in hydroxyproline, and histological assessments were performed. Besides, intraoperative oxygen saturation and postoperative arterial blood gas parameters were also compared. On the first postoperative day, both arterial oxygen tension (PO2) and oxygen saturation (SO2) in the PEEP group were significantly higher than in the ZEEP group. On the seventh postoperative day, the bursting pressures of the anastomoses were significantly higher in the PEEP group, however the hydroxyproline content was significantly lower in the PEEP group than that in the ZEEP group. At day 7, PEEP group was significantly associated with increased neoangiogenesis compared with the ZEEP group. The anastomotic healing process is positively influenced by the intraoperative PEEP application.

Research paper thumbnail of Use of a circular external skeletal fixator to treat comminuted metacarpal and tibial fractures in six calves

The Veterinary record, Jan 6, 2008

A circular external skeletal fixator system was used to fix long-bone fractures in six calves, th... more A circular external skeletal fixator system was used to fix long-bone fractures in six calves, three with tibial fractures and three with metacarpal fractures. An Ilizarov apparatus of appropriate diameter, depending on the calves' bodyweight, was used. In the postoperative period, clinical and radiological examinations were carried out to assess fracture healing, the stability of the fixator and when the calves could make full use of the limb. The fixator was tolerated well, the fractures healed and the fixators maintained rigid stability. The calves were able to use their limbs fully after between 15 and 27 days.

Research paper thumbnail of Effects of Vascular Bundle Implantation on Autograft, Fresh-Frozen Allograft, and Xenograft Incorporation in a Rabbit Model

Orthopedics, 2008

This study examined the effects of vascular bundle implantation into a bone graft. Vascularized a... more This study examined the effects of vascular bundle implantation into a bone graft. Vascularized and nonvascularized autografts, allografts, and xenografts were placed inside defects in the proximal tibia in rabbits. Evaluation using radiographs, magnetic resonance imaging, bone scintigraphy, and microscopy showed autografts fused more rapidly than allografts and xenografts, and the majority of the vascularized grafts were incorporated completely. Autografts emerged as the gold standard. These findings indicate vessel implantation enhances and accelerates vascularization, new bone formation, and incorporation in autografts, allografts, and xenografts.

Research paper thumbnail of Scintigraphic imaging of radiolabelled drug delivery systems in rabbits with arthritis

International Journal of Pharmaceutics, 2005

This paper describes a novel approach for designing drug delivery systems for intra-articular (i.... more This paper describes a novel approach for designing drug delivery systems for intra-articular (i.a.) treatment of rheumatoid arthritis. Retention of these systems was evaluated by radiolabeling with Tc-99m and gamma scintigraphy in arthritic rabbits. Liposome, niosome, lipogelosome and niogelosome formulations of Diclofenac Sodium (DFNa) have been prepared and drug release properties and in vitro characterisation studies have been carried out. According to characterisation results L1 (DMPC: CHOL: DCP (7:1:2)), L1J1 (DMPC: CHOL: DCP (7:1:2) in C-940 1:1 (w/w)), N (SUR I: CHOL: DCP (7:1:2)) and NJ1 (SUR I: CHOL: DCP (7:1:2) in C-940 1:1 (w/w)) formulations were chosen for the further studies. Retention time of these formulations was evaluated by gamma scintigraphic imaging studies. Rabbits with antigen-induced arthritis were injected intra-articularly with Tc-99m labelled drug delivery systems. Serial scintigraphic images were obtained to investigate the retentions of labelled drug delivery systems in the arthritic joints and choose a suitable formulation for the treatment protocol of arthritis.

Research paper thumbnail of The effect of gamma radiation sterilization on dental biomaterials

Tissue Engineering and Regenerative Medicine, 2014

Biomaterials are used in the field of bone and tissue engineering, orthopaedics and dentistry. De... more Biomaterials are used in the field of bone and tissue engineering, orthopaedics and dentistry. Dental biomaterials including commercially available biodegradable materials act as physical barriers to help quicker healing while stimulating the regeneration of periodontal tissues, which is defined as Guided Tissue Regeneration (GTR). Amongst natural and synthetic biomaterials, collagen and aliphatic polyesters, such as polylactic acid (PLA) and poly (lactic-co-glycolic) acid (PLGA) are the most frequently used biomaterials for regenerative therapies due to their excellent biocompatibility and biodegradability. Due to their resorption in the body and interaction with biological systems, the GTR membranes must be sterile and pyrogen free. The sterility and apyrogenicity of the GTR membranes before human use is a regulatory requirement, however the sterilization of biomaterials is challenging due to the physicochemical changes and toxic residues with the commonly used sterilization techniques. The purpose of the present study was to evaluate the effect of gamma radiation and ethylene oxide sterilization on dental biomaterials with analytical, microbiological and histological examinations. PLGA-based GTR dental biomaterial is selected as the most gamma stable membrane according to the FTIR, DSC, TGA, and SEM results. This dental membrane was sterilized with ethylene oxide (EtO) and the effect of sterilization method on PLGA-based membrane was also investigated. Animal experiments were carried out to evaluate the regenerative properties and inflammatory responses of gamma and EtO sterilized PLGA-based GTR membrane after implantation. Histological examinations showed that resorption and bone formation of gamma sterilized PLGA-based GTR membrane was completed in 12 weeks without any inflammatory response; while only 60.095 ± 2.019% of new bone formation was observed with EtO sterilized one. Gamma sterilized PLGA membrane had significantly faster (P < 0.05) resorption and bone formation in comparison with EtO sterilization. In conclusion, the PLGA-based biomaterials could be sterilized safely and time-and cost-effectively with validated radiation doses for the tissue engineering applications.