Hassan Abol-Enein - Academia.edu (original) (raw)

Papers by Hassan Abol-Enein

International Urology and Nephrology, Jan 13, 2007

Background A high serum level of female protein (FP), found to be a constituent of Syrian hamster... more Background A high serum level of female protein (FP), found to be a constituent of Syrian hamster amyloid was associated with enhanced amyloidosis. In this work, we studied the sex-limited factors in the induction of amyloidosis in Syrian hamsters infected with either Schistosoma mansoni or S. hematobium cercariae. Methods Hamsters were infected with different species of schistosome cercariae and sacrificed after different time periods of infection. Kidney and liver specimens were processed in paraffin, stained with Congo-red and examined by ordinary light and polarized light microscopy. Results Statistical analysis showed a significant difference in intensity of kidney and liver amyloid deposits (P < 0.002 and < 0.007, respectively) between females and male hamsters with extensive deposits in the former. Amyloid deposits were correlated significantly to the duration of infection (P < 0.001) than the worm load recovered. Conclusion We conclude that, in a hamster model, Schistosoma-induced amyloidosis is enhanced in female compared to male hamsters. This may be due to the high serum level of FP that is normally detected in females. As an experimental model for schistosomal nephropathy, we recommend to use male hamsters instead of females to minimize the effect of amyloid deposits, which may mask other pathological changes associated with schistosomal infection.

Urolithiasis, Mar 13, 2023

To investigate the association between metabolic urinary abnormalities and urinary tract infectio... more To investigate the association between metabolic urinary abnormalities and urinary tract infection (UTI) and the stone recurrence status in patients undergoing percutaneous nephrolithotomy (PCNL). A prospective evaluation was performed for patients who underwent PCNL between November 2019 and November 2021 and met the inclusion criteria. Patients with previous stone interventions were classified as recurrent stone formers. Before PCNL, a 24 h metabolic stone workup and midstream urine culture (MSU-C) were done. Renal pelvis (RP-C) and stones (S-C) cultures were collected during the procedure. The association between the metabolic workup and UTI results with stone recurrence was evaluated using univariate and multivariate analyses. The study included 210 patients. UTI factors that showed significant association with stone recurrence included positive S-C [51 (60.7%) vs 23 (18.2%), p < 0.001], positive MSU-C [37 (44.1%) vs 30 (23.8%), p = 0.002], and positive RP-C [17 (20.2%) vs 12 (9.5%), p = 0.03]. Other factors were mean ± SD GFR (ml/min) (65 ± 13.1 vs 59.5 ± 13.1, p = 0.003), calcium-containing stones [47 (55.9%) vs 48 (38.1%), p = 0.01], median (IQR) urinary citrate levels (mg/day) [333 (123-512.5) vs 221.5 (120.3-412), p = 0.04], and mean ± SD urinary pH (6.1 ± 1 vs 5.6 ± 0.7, p < 0.001). On multivariate analysis, only positive S-C was the significant predictor of stone recurrence (odds ratio: 9.9, 95% confidence interval [CI] (3.8-28.6), p < 0.001). Positive S-C, and not metabolic abnormalities, was the only independent factor associated with stone recurrence. A focus on preventing UTI might prevent further stone recurrence.

The Journal of Urology, Apr 1, 2018

BJUI, Sep 10, 2007

All patients presented with gross haematuria. There was non-muscle-invasive disease in two patien... more All patients presented with gross haematuria. There was non-muscle-invasive disease in two patients who were treated by transurethral resection and adjuvant intravesical bacille Calmette-Guérin immunotherapy. One patient died 24 months later due to complications of end-stage renal disease. To date the second patient is alive and free of the recurrence. Five recipients with muscle-invasive disease had a radical cystectomy and orthotopic bladder substitution. The mean (SD) time to the last follow-up or death was 14.6 (3.1) months. Three patients died with stable graft function; two from distant metastasis and one from a cerebrovascular stroke. The remaining two patients are still alive, free of disease and with good graft function. CONCLUSIONS Urothelial bladder tumours are generally uncommon. The presence of haematuria in renal allograft recipients should be thoroughly investigated. Early diagnosis and prompt treatment are required for managing such tumours, because they are aggressive. Orthotopic bladder substitution is feasible with a good functional outcome for patients in whom cystectomy is indicated.

European Urology Supplements, Mar 1, 2013

PubMed, 2004

Background: Transitional cell carcinoma (TCC) is the fifth most common solid malignancy in the U.... more Background: Transitional cell carcinoma (TCC) is the fifth most common solid malignancy in the U.S. Unfortunately, recurrence, invasion and metastasis are characteristic of bladder cancer. Additional studies to define factors involved in bladder cancer progression would facilitate the design of molecularly-based diagnostic and therapeutic approaches. Materials and methods: Genomic alterations contribute to bladder cancer tumorigenesis. To identify gains or losses of chromosomal regions involved in invasive bladder cancer, we performed representational difference analysis (RDA) using DNA from transitional cell carcinoma (TCC) vs. DNA from adjacent non-tumor tissue. Results: Our genome-wide analysis allowed the identification of 61 loci that show loss or gain in bladder cancer. Many of the identified loci are present within large chromosomal regions previously found to undergo alterations in bladder cancer, as well as defining regions previously not associated with bladder cancer. Genomic changes identified by RDA are found in multiple tumors. Conclusion: Identification of gene losses and gains provides a means to uncover novel candidate tumor suppressor genes and oncogenes. RDA is a simple, inexpensive and efficient approach for the detection of specific gene changes in bladder cancer.

European Urology Supplements, Apr 1, 2010

The Journal of Urology, Apr 1, 2006

European urology open science, Jul 1, 2020

European Urology Supplements, Mar 1, 2009

Introduction & Objectives: Traditionally, intraperitoneal bladder perforations by trauma or iatro... more Introduction & Objectives: Traditionally, intraperitoneal bladder perforations by trauma or iatrogenic have been treated by open or laparoscopic surgery. Additionally, transvesical access to the peritoneal cavity has been reported to be feasible and useful for natural orifice translumenal endoscopic surgery (NOTES) but would be enhanced by a reliable method to close the vesicotomy. We assessed the feasibility and safety of endoscopic closure method of vesical perforations using an endoscopic suturing kit. Material & Methods: Endoscopic closure of vesical perforations was performed in six anesthetized female pigs. Under control of cystoscopy, a full-thickness longitudinal incision was created on the bladder dome. Then, the endoscopic closure of the perforation was performed with the endoscopic suturing kit. The acute quality of sealing was immediately tested by distending the bladder with methylene-blue dye under laparoscopic control (in two animals). Without a bladder catheter, the animals were monitored daily for 2 weeks. Results: Endoscopic closure of bladder perforation was carried out easily and quickly in all animals. The laparoscopic view revealed no acute leak of methylene-blue dye after distension of the bladder. After recovery of anesthesia, the pigs began to void normally and no adverse event occurred. Postmortem examination revealed complete healing of vesical incision. conclusions: This study demonstrates the feasibility and the safety of endoscopic closure of vesical perforations with an endoscopic suturing kit in a survival porcine model. This study provides support for clinical studies using endoscopic closure of the bladder to design a new era in management of bladder rupture and transvesical port in NOTES procedures.

The Journal of Urology, Apr 1, 2016

detected pulmonary embolism) and timing of TEE (before or after (< or > 30 days) RC) and Khorana ... more detected pulmonary embolism) and timing of TEE (before or after (< or > 30 days) RC) and Khorana score (established for cancer patients treated with chemotherapy, based on baseline hemoglobin, platelet and leukocyte counts, BMI and tumor site) was determined for all patients. All patients received TEE prophylaxis during the early post-RC period. Multivariate analysis was performed on 827 patients. Kaplan Meier survival curves and log rank test were used to compare survival between patients who developed TEE and those who did not. RESULTS: The Khorana criteria indicated intermediate TEE risk in most patients. Khorana risk score was 1 or 2 in 88% of patients. Overall, the incidence of TEE in patients was 15%. 59 TEE were detected pre-operatively (7.1%), 21 early within 30 days of RC (2.5%) and 36 late post-operatively (4.3%). 32% of the TEE events were detected incidentally by imaging, 68% were detected clinically. Median overall survival of patients who developed TEE was 28 months compared to 71 months for those who did not develop TEE (p¼0.012). CONCLUSIONS: This multi-centre retrospective study suggests that TEE are very common in bladder cancer patients undergoing NAC before and after RC and they associate with poorer survival. Further investigation with a prospective prevention trial for the period of NAC is warranted.

European Urology Supplements, Feb 1, 2012

Objectives: To present treatments for kidney preservation in the management of emphysematous pyel... more Objectives: To present treatments for kidney preservation in the management of emphysematous pyelonephritis (EPN), and to evaluate the functional outcome of preserved kidneys during the follow-up. Patients and methods: The computerized files of patients with EPN from 2000 to 2010 were reviewed. After initial resuscitation, ultrasonography-guided percutaneous tubes were placed for drainage of infected fluid and gas. A radio-isotopic renal scan was done after stabilization of the patients' condition. Preservation of the affected kidney was attempted when the differential function was >10%. A renal isotopic scan was taken during the follow-up to evaluate renographic changes in preserved kidneys. Results: The study included 33 kidneys in 30 consecutive patients (mean age 51.7 years, SD 10.9). Kidney preservation was applicable for 23 kidneys (20 patients). Preservation methods included percutaneous nephrostomy for 12, percutaneous tube drain for two and conservative treatment for nine kidneys (six patients). Nephrectomy was performed for 10 kidneys (emergency in three and delayed in seven). The frequency of post-treatment septic shock after kidney preservation (10%) was significantly lower than after nephrectomy (20%, P = 0.005). The overall mortality rate

Genes, Sep 10, 2022

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Clinical Genitourinary Cancer, Apr 1, 2022

In this study we aim to compare clinicopathological characteristics and cancer specific survival ... more In this study we aim to compare clinicopathological characteristics and cancer specific survival between patients treated with radical cystectomy for pure squamous cell carcinoma (SCC) and urothelial carcinoma with squamous differentiation (SqD). We reviewed data of 1737 consecutive patients treated with radical cystectomy and urinary diversion between January 2004 and February 2014. Only patients with pure SCC or SqD were included in the analysis. Squamous differentiation was defined as intercellular bridges or keratinization in the tumor. Clinicopathological data and recurrence free survival (RFS) were compared between patients diagnosed with SCC and SqD. SCC and SqD were found in 318 and 223 patients, respectively. Mean age was 57 ± 8.3 years in SCC and 58.8 ± 7.8 in SqD (P = .008). A higher proportion of female patients was observed in SCC group compared to SqD (31.8% vs. 22% P < .0001). Patients with SqD were more likely to have extravesical (58.3% vs. 46.2%: P = .006) and nodal positive disease (34.5% vs. 14.5%: P < .0001) than pure SCC patients. Bilharzial eggs were found in 61% of SCC vs. 46% of SqD (P = .001).; The median (IQR) follow up period for SCC and SqD was 63 (12-112) months and 23 months (9-74.7), respectively. The 5-year RFS for SCC and SqD were 77% and 59.8 %, respectively (P < .0001).; Multivariate cox regression analysis identified advanced pT stage (OR: 1.9, 95% CI: 1.3-2.86, P = .0001), nodal positive disease (OR: 1.6, 95% CI: 1.1-2.48, P = .01) and SqD histology (OR: 1.6, 95% CI: 1.14-2.31, P = .007 as independent predictors of 5-year RFS. Patient with SCC had significantly higher 5-year RFS in comparison to SqD. The higher rate of extravesical disease and lymph node metastasis in SqD patients is indicative of aggressive behavior of this histologic type.

Clinical Genitourinary Cancer, Jun 1, 2022

BACKGROUND Till now, no definite clinical or laboratory marker can predict the recurrence or prog... more BACKGROUND Till now, no definite clinical or laboratory marker can predict the recurrence or progression of T1 G3 urothelial carcinoma (UC). Genetic aberrations of the chromatin remodeling genes and sister chromatid cohesion and segregation (SCCS) were identified in UC. Here we investigated the impact of novel miRNAs and their targeted expressed SCCS and chromatin remodeling genes on T1G3 UC response to Bacillus Calmette-Guérin (BCG) therapy. METHODS One hundred tissue samples were obtained from NMIBC patients. Gene expression and immunohistochemical assay of STAG2, ARID1A, NCOR1and UTX were assessed. MiRNA analysis for their targeting miRNAs (miR-21, miR-31, Let7a and miR-199a) was carried out. Assessed genes were compared between responders and no responders to BCG. Univariate and multivariate analysis of predictors of disease recurrence and progression were performed using cox regression analysis. RESULTS Thirty-two and 22 patients developed recurrence and progression to MIBC (BCG non-responders). BCG non-responders showed statistically significant higher expression of miR-21 and their targeted STAG2, miR-199a and NCOR1 gene (P < .001), and lower expression of miR-31, Let7a, ARID1A and UTX genes (P < .001). Higher miR-199a (P = .006) and lower miR-31 (P = .01), ARID1A (P = .008) and UTX (P = .03) were independent predictor of higher tumor recurrence. Recurrent disease (P = .003), higher expression of STAG2 (P = .01), NCOR1 (P = .01) and miR-21 (P = .03) genes and lower expression of miR-31 (P = .02), Let7a (P = .04) and ARID1A (P = .04) genes were the independent predictor of disease progression. CONCLUSION Upregulation of STAG2 and NCOR1 and down regulation of ARID1A and UTX genes and their targeting miRNAs were associated with UC non-response to BCG.

African Journal of Urology, 2003

Scandinavian journal of urology, Oct 16, 2020

Purpose: To evaluate the predictors of post-ileal conduit (IC) parastomal hernia (PSH) based on a... more Purpose: To evaluate the predictors of post-ileal conduit (IC) parastomal hernia (PSH) based on a standard grading methodology and according to the patients reported outcome measures (PROM). Methods: A prospective evaluation for patients with IC attending their scheduled follow-up was conducted between December 2013 and October 2015. The hernia stage was determined according to the European Hernia Society (EHS) classification as types I and II included defect size < 5 cm without and with a concomitant incisional hernia, respectively. Types III and IV included defect size > 5 cm without and with a concomitant incisional hernia (high-grade hernia). The evaluation was performed by a non-contrast CT scan. PROM were defined as symptomatic if there were hernia-related abdominal discomfort, appliance problems, and/or bowel complications. Perioperative parameters were modeled for prediction of high-grade and PROM outcomes. Results: PSH was diagnosed in 138 (39.9%) patients, symptomatic in 119 (34.4%) and high-grade in 59 (17%). Independent predictors of radiologically diagnosed PSH were hypoalbuminemia (odds ratio [OR]: 1.

African Journal of Urology, 2002

Journal of Urology, 2021

INTRODUCTION AND OBJECTIVE:To assess the predictive performance of different immunological marker... more INTRODUCTION AND OBJECTIVE:To assess the predictive performance of different immunological markers on BCG-response in high risk NMIBC BCG-naive patients.METHODS:Patients who underwent TURBT for NMI...

Journal of Urology, 2020

INTRODUCTION AND OBJECTIVE:In modern exstrophy practice, urinary diversion is typically reserved ... more INTRODUCTION AND OBJECTIVE:In modern exstrophy practice, urinary diversion is typically reserved for patients with multiple failed attempts of reconstruction. Colonic diversion is associated with a...