Gary Hein - Academia.edu (original) (raw)

Papers by Gary Hein

Research paper thumbnail of EC03-1568 Grasshopper Identification Guide for Rangeland and Pasture Summer Feeding Species

Research paper thumbnail of EC03-1569 Grasshopper Identification Guide for Cropland Grasshoppers: Summer Feeding Species

Research paper thumbnail of EC03-1567 Grasshopper Identification Guide for Rangeland Grasshoppers: Fall/Spring Feeding Species

Research paper thumbnail of NF95-217 Sugarbeet Root Maggot Management

Research paper thumbnail of Wheat transcriptomic responses to extended feeding by wheat curl mites

Scientific Reports

The economic importance of wheat and its contribution to human and livestock diets has been alrea... more The economic importance of wheat and its contribution to human and livestock diets has been already demonstrated. However, wheat production is impacted by pests that induce yield reductions. Among these pests, wheat curl mite (WCM, Aceria tosichella Keifer) impacts wheat all around the world. WCM are tiny pests that feed within the whorl of developing leaves, and their feeding causes leaf curling by preventing them from unfurling. The curling of the leaves provides a protective niche for the WCM. Additionally, WCM are also the vector of serious viruses in wheat. Little is known regarding the impact of the WCM on wheat transcriptome, and to date, only one article has been published describing the wheat transcriptomic changes after 1 day of WCM feeding. To better understand the wheat transcriptome variation after extended feeding by WCM [10 days post infestation (dpi)], we used an RNA-seq approach. We collected WCM-infested and uninfested leaves from two wheat cultivars: Byrd (WCM res...

Research paper thumbnail of Farm Management Practices Used by Wheat Producers in the Western Great Plains: Estimating Their Productivity and Profitability

Journal of Applied Farm Economics, 2020

for additional information. This is an Open Access journal. This means that it uses a funding mod... more for additional information. This is an Open Access journal. This means that it uses a funding model that does not charge readers or their institutions for access. Readers may freely read, download, copy, distribute, print, search, or link to the full texts of articles. This journal is covered under the CC BY-NC-ND license.

Research paper thumbnail of Hitchhiking or hang gliding? Dispersal strategies of two cereal-feeding eriophyoid mite species

Experimental and Applied Acarology, 2021

Dispersal shapes the dynamics of populations, their genetic structure and species distribution; t... more Dispersal shapes the dynamics of populations, their genetic structure and species distribution; therefore, knowledge of an organisms’ dispersal abilities is crucial, especially in economically important and invasive species. In this study, we investigated dispersal strategies of two phytophagous eriophyoid mite species: Aceria tosichella (wheat curl mite, WCM) and Abacarus hystrix (cereal rust mite, CRM). Both species are obligatory plant parasites that infest cereals and are of economic significance. We investigated their dispersal success using different dispersal agents: wind and vectors. We hypothesised that in both mite species the main mode of dispersal is moving via wind, whereas phoretic dispersal is rather accidental, as the majority of eriophyoid mite species do not possess clear morphological or behavioural adaptations for phoresy. Results confirmed our predictions that both species dispersed mainly with wind currents. Additionally, WCM was found to have a higher dispersa...

Research paper thumbnail of Registration of ‘NE10589’ (Husker Genetics Brand Ruth) hard red winter wheat

Journal of Plant Registrations, 2020

With climate variation common in the U.S. Great Plains and particularly in Nebraska, wheat grower... more With climate variation common in the U.S. Great Plains and particularly in Nebraska, wheat growers prefer broadly adapted cultivars. 'NE10589' (Reg. no. CV-1165, PI 675998) hard red winter wheat (Triticum aestivum L.) was developed cooperatively by the Nebraska Agricultural Experiment Station and the USDA-ARS and released in January 2015 by the developing institutions. NE10589 was released primarily as a broadly adapted semi-dwarf cultivar for its superior performance under rainfed conditions throughout Nebraska and adjacent areas of the Great Plains. Its broad adaptation ensures that it will perform well under the typical environmental fluctuations that occur in Nebraska. NE10589 was selected from the cross 'OK98697'/'Jagalene'//'Camelot', where the pedigree of OK98697 is 'TAM 200'/'HBB313E'//'2158'. The F 2 to F 3 generations were advanced using the bulk breeding method at the Eastern Nebraska Research and Extension Center near Mead, NE, in 2006-2007. In 2007-2008, single F 3:4 head rows were grown for selection. There was no further selection within the line thereafter. The F 3:5 was evaluated as a single four-row plot at Lincoln, NE, and a single row at Mead, NE, in 2009. In 2010, it was assigned the experimental line number NE10589.

Research paper thumbnail of Octapartite negative-sense RNA genome of High Plains wheat mosaic virus encodes two suppressors of RNA silencing

Virology, May 1, 2018

High Plains wheat mosaic virus (HPWMoV, genus Emaravirus; family Fimoviridae), transmitted by the... more High Plains wheat mosaic virus (HPWMoV, genus Emaravirus; family Fimoviridae), transmitted by the wheat curl mite (Aceria tosichella Keifer), harbors a monocistronic octapartite single-stranded negative-sense RNA genome. In this study, putative proteins encoded by HPWMoV genomic RNAs 2-8 were screened for potential RNA silencing suppression activity by using a green fluorescent protein-based reporter agroinfiltration assay. We found that proteins encoded by RNAs 7 (P7) and 8 (P8) suppressed silencing induced by single- or double-stranded RNAs and efficiently suppressed the transitive pathway of RNA silencing. Additionally, a Wheat streak mosaic virus (WSMV, genus Tritimovirus; family Potyviridae) mutant lacking the suppressor of RNA silencing (ΔP1) but having either P7 or P8 from HPWMoV restored cell-to-cell and long-distance movement in wheat, thus indicating that P7 or P8 rescued silencing suppressor-deficient WSMV. Furthermore, HPWMoV P7 and P8 substantially enhanced the pathogen...

Research paper thumbnail of NF328 A Guide to Grasshopper Control in Cropland

Grasshoppers have been a major concern to farmers and ranchers since Nebraska was first settled. ... more Grasshoppers have been a major concern to farmers and ranchers since Nebraska was first settled. The potential for devastation, while still serious, is not as great as it was 100 years ago because many of the prime grasshopper breeding areas along the eastern slopes of the Rocky Mountains are now under tillage. Statewide, grasshopper populations fluctuate in cycles with large numbers occurring for two to four years, followed by moderate numbers for several years. While grasshoppers can be found across the state, most damage occurs in areas with less than 25 inches of annual rainfall. In most years, the western half of Nebraska falls into this higher risk category and is most susceptible to severe grasshopper outbreaks.

Research paper thumbnail of EC94-1559-D Pesticide Use on Specialty Crops in Nebraska 1992

Research paper thumbnail of NF97-328 A Guide to Grasshopper Control in Cropland (Revised May 2004)

Grasshoppers have been a major concern to farmers and ranchers since Nebraska was first settled. ... more Grasshoppers have been a major concern to farmers and ranchers since Nebraska was first settled. The potential for devastation, while still serious, is not as great as it was 100 years ago because many of the prime grasshopper breeding areas along the eastern slopes of the Rocky Mountains are now under tillage. Statewide, grasshopper populations fluctuate in cycles with large numbers occurring for two to four years, followed by moderate numbers for several years. While grasshoppers can be found across the state, most damage occurs in areas with less than 25 inches of annual rainfall. In most years, the western half of Nebraska falls into this higher risk category and is most susceptible to severe grasshopper outbreaks.

Research paper thumbnail of G92-1108 Evaluating Corn Rootworm Soil Insecticide Performance

Research paper thumbnail of EC04-1571 Pesticide Use on Specialty Crops in Nebraska - 1999

Research paper thumbnail of G08-1823 Barley Yellow Dwarf of Wheat, Barley, and Oats

Research paper thumbnail of EC08-1871 Managing Wheat Streak Mosaic

Research paper thumbnail of High Plains Ag Lab Research Update 2003

Research paper thumbnail of Genetically Engineered Bt Corn and Range Expansion of the Western Bean Cutworm (Lepidoptera: Noctuidae) in the United States: A Response to Greenpeace Germany

Journal of Integrated Pest Management, 2011

Mention of a proprietary product does not constitute an endorsement or a recommendation for its u... more Mention of a proprietary product does not constitute an endorsement or a recommendation for its use by the universities associated with this research.

Research paper thumbnail of EC04-183A Brown Mustard Production

Research paper thumbnail of Biological properties of isolates ofTriticum mosaic virusfrom the Great Plains states of the USA

Canadian Journal of Plant Pathology, 2014

ABSTRACT Triticum mosaic virus (TriMV) is a recently discovered virus infecting wheat. A total of... more ABSTRACT Triticum mosaic virus (TriMV) is a recently discovered virus infecting wheat. A total of 170 isolates of TriMV, collected in 2010 and 2011 from wheat (Triticum aestivum L) or jointed goatgrass (Aegilops cylindrica Host) from Colorado, Kansas, Nebraska and South Dakota, were compared with the 06-123 Kansas isolate. These isolates were compared for the percentage of infected plants in N28Ht maize, ‘Gallatin’ barley and ‘Mace’ wheat (with temperature-sensitive resistance to Wheat streak mosaic virus (WSMV). The isolates were also compared for the effect of inoculum virus titre on the percentage of infected plants in WSMV-susceptible ‘Tomahawk’ wheat by mechanically inoculating the cultivar using 1:10 w/v, 1:300 v/v or 1:600 v/v dilutions of extracts. None of the isolates infected N28Ht maize but all isolates infected ‘Gallatin’ barley, ‘Mace’ and ‘Tomahawk’ wheat. Some isolates from Colorado, Kansas and Nebraska had low relative titre in wheat compared with the 06-123 isolate. This information is important in critical selection of TriMV isolates for use in greenhouse and field studies and in resistance screening protocols.

Research paper thumbnail of EC03-1568 Grasshopper Identification Guide for Rangeland and Pasture Summer Feeding Species

Research paper thumbnail of EC03-1569 Grasshopper Identification Guide for Cropland Grasshoppers: Summer Feeding Species

Research paper thumbnail of EC03-1567 Grasshopper Identification Guide for Rangeland Grasshoppers: Fall/Spring Feeding Species

Research paper thumbnail of NF95-217 Sugarbeet Root Maggot Management

Research paper thumbnail of Wheat transcriptomic responses to extended feeding by wheat curl mites

Scientific Reports

The economic importance of wheat and its contribution to human and livestock diets has been alrea... more The economic importance of wheat and its contribution to human and livestock diets has been already demonstrated. However, wheat production is impacted by pests that induce yield reductions. Among these pests, wheat curl mite (WCM, Aceria tosichella Keifer) impacts wheat all around the world. WCM are tiny pests that feed within the whorl of developing leaves, and their feeding causes leaf curling by preventing them from unfurling. The curling of the leaves provides a protective niche for the WCM. Additionally, WCM are also the vector of serious viruses in wheat. Little is known regarding the impact of the WCM on wheat transcriptome, and to date, only one article has been published describing the wheat transcriptomic changes after 1 day of WCM feeding. To better understand the wheat transcriptome variation after extended feeding by WCM [10 days post infestation (dpi)], we used an RNA-seq approach. We collected WCM-infested and uninfested leaves from two wheat cultivars: Byrd (WCM res...

Research paper thumbnail of Farm Management Practices Used by Wheat Producers in the Western Great Plains: Estimating Their Productivity and Profitability

Journal of Applied Farm Economics, 2020

for additional information. This is an Open Access journal. This means that it uses a funding mod... more for additional information. This is an Open Access journal. This means that it uses a funding model that does not charge readers or their institutions for access. Readers may freely read, download, copy, distribute, print, search, or link to the full texts of articles. This journal is covered under the CC BY-NC-ND license.

Research paper thumbnail of Hitchhiking or hang gliding? Dispersal strategies of two cereal-feeding eriophyoid mite species

Experimental and Applied Acarology, 2021

Dispersal shapes the dynamics of populations, their genetic structure and species distribution; t... more Dispersal shapes the dynamics of populations, their genetic structure and species distribution; therefore, knowledge of an organisms’ dispersal abilities is crucial, especially in economically important and invasive species. In this study, we investigated dispersal strategies of two phytophagous eriophyoid mite species: Aceria tosichella (wheat curl mite, WCM) and Abacarus hystrix (cereal rust mite, CRM). Both species are obligatory plant parasites that infest cereals and are of economic significance. We investigated their dispersal success using different dispersal agents: wind and vectors. We hypothesised that in both mite species the main mode of dispersal is moving via wind, whereas phoretic dispersal is rather accidental, as the majority of eriophyoid mite species do not possess clear morphological or behavioural adaptations for phoresy. Results confirmed our predictions that both species dispersed mainly with wind currents. Additionally, WCM was found to have a higher dispersa...

Research paper thumbnail of Registration of ‘NE10589’ (Husker Genetics Brand Ruth) hard red winter wheat

Journal of Plant Registrations, 2020

With climate variation common in the U.S. Great Plains and particularly in Nebraska, wheat grower... more With climate variation common in the U.S. Great Plains and particularly in Nebraska, wheat growers prefer broadly adapted cultivars. 'NE10589' (Reg. no. CV-1165, PI 675998) hard red winter wheat (Triticum aestivum L.) was developed cooperatively by the Nebraska Agricultural Experiment Station and the USDA-ARS and released in January 2015 by the developing institutions. NE10589 was released primarily as a broadly adapted semi-dwarf cultivar for its superior performance under rainfed conditions throughout Nebraska and adjacent areas of the Great Plains. Its broad adaptation ensures that it will perform well under the typical environmental fluctuations that occur in Nebraska. NE10589 was selected from the cross 'OK98697'/'Jagalene'//'Camelot', where the pedigree of OK98697 is 'TAM 200'/'HBB313E'//'2158'. The F 2 to F 3 generations were advanced using the bulk breeding method at the Eastern Nebraska Research and Extension Center near Mead, NE, in 2006-2007. In 2007-2008, single F 3:4 head rows were grown for selection. There was no further selection within the line thereafter. The F 3:5 was evaluated as a single four-row plot at Lincoln, NE, and a single row at Mead, NE, in 2009. In 2010, it was assigned the experimental line number NE10589.

Research paper thumbnail of Octapartite negative-sense RNA genome of High Plains wheat mosaic virus encodes two suppressors of RNA silencing

Virology, May 1, 2018

High Plains wheat mosaic virus (HPWMoV, genus Emaravirus; family Fimoviridae), transmitted by the... more High Plains wheat mosaic virus (HPWMoV, genus Emaravirus; family Fimoviridae), transmitted by the wheat curl mite (Aceria tosichella Keifer), harbors a monocistronic octapartite single-stranded negative-sense RNA genome. In this study, putative proteins encoded by HPWMoV genomic RNAs 2-8 were screened for potential RNA silencing suppression activity by using a green fluorescent protein-based reporter agroinfiltration assay. We found that proteins encoded by RNAs 7 (P7) and 8 (P8) suppressed silencing induced by single- or double-stranded RNAs and efficiently suppressed the transitive pathway of RNA silencing. Additionally, a Wheat streak mosaic virus (WSMV, genus Tritimovirus; family Potyviridae) mutant lacking the suppressor of RNA silencing (ΔP1) but having either P7 or P8 from HPWMoV restored cell-to-cell and long-distance movement in wheat, thus indicating that P7 or P8 rescued silencing suppressor-deficient WSMV. Furthermore, HPWMoV P7 and P8 substantially enhanced the pathogen...

Research paper thumbnail of NF328 A Guide to Grasshopper Control in Cropland

Grasshoppers have been a major concern to farmers and ranchers since Nebraska was first settled. ... more Grasshoppers have been a major concern to farmers and ranchers since Nebraska was first settled. The potential for devastation, while still serious, is not as great as it was 100 years ago because many of the prime grasshopper breeding areas along the eastern slopes of the Rocky Mountains are now under tillage. Statewide, grasshopper populations fluctuate in cycles with large numbers occurring for two to four years, followed by moderate numbers for several years. While grasshoppers can be found across the state, most damage occurs in areas with less than 25 inches of annual rainfall. In most years, the western half of Nebraska falls into this higher risk category and is most susceptible to severe grasshopper outbreaks.

Research paper thumbnail of EC94-1559-D Pesticide Use on Specialty Crops in Nebraska 1992

Research paper thumbnail of NF97-328 A Guide to Grasshopper Control in Cropland (Revised May 2004)

Grasshoppers have been a major concern to farmers and ranchers since Nebraska was first settled. ... more Grasshoppers have been a major concern to farmers and ranchers since Nebraska was first settled. The potential for devastation, while still serious, is not as great as it was 100 years ago because many of the prime grasshopper breeding areas along the eastern slopes of the Rocky Mountains are now under tillage. Statewide, grasshopper populations fluctuate in cycles with large numbers occurring for two to four years, followed by moderate numbers for several years. While grasshoppers can be found across the state, most damage occurs in areas with less than 25 inches of annual rainfall. In most years, the western half of Nebraska falls into this higher risk category and is most susceptible to severe grasshopper outbreaks.

Research paper thumbnail of G92-1108 Evaluating Corn Rootworm Soil Insecticide Performance

Research paper thumbnail of EC04-1571 Pesticide Use on Specialty Crops in Nebraska - 1999

Research paper thumbnail of G08-1823 Barley Yellow Dwarf of Wheat, Barley, and Oats

Research paper thumbnail of EC08-1871 Managing Wheat Streak Mosaic

Research paper thumbnail of High Plains Ag Lab Research Update 2003

Research paper thumbnail of Genetically Engineered Bt Corn and Range Expansion of the Western Bean Cutworm (Lepidoptera: Noctuidae) in the United States: A Response to Greenpeace Germany

Journal of Integrated Pest Management, 2011

Mention of a proprietary product does not constitute an endorsement or a recommendation for its u... more Mention of a proprietary product does not constitute an endorsement or a recommendation for its use by the universities associated with this research.

Research paper thumbnail of EC04-183A Brown Mustard Production

Research paper thumbnail of Biological properties of isolates ofTriticum mosaic virusfrom the Great Plains states of the USA

Canadian Journal of Plant Pathology, 2014

ABSTRACT Triticum mosaic virus (TriMV) is a recently discovered virus infecting wheat. A total of... more ABSTRACT Triticum mosaic virus (TriMV) is a recently discovered virus infecting wheat. A total of 170 isolates of TriMV, collected in 2010 and 2011 from wheat (Triticum aestivum L) or jointed goatgrass (Aegilops cylindrica Host) from Colorado, Kansas, Nebraska and South Dakota, were compared with the 06-123 Kansas isolate. These isolates were compared for the percentage of infected plants in N28Ht maize, ‘Gallatin’ barley and ‘Mace’ wheat (with temperature-sensitive resistance to Wheat streak mosaic virus (WSMV). The isolates were also compared for the effect of inoculum virus titre on the percentage of infected plants in WSMV-susceptible ‘Tomahawk’ wheat by mechanically inoculating the cultivar using 1:10 w/v, 1:300 v/v or 1:600 v/v dilutions of extracts. None of the isolates infected N28Ht maize but all isolates infected ‘Gallatin’ barley, ‘Mace’ and ‘Tomahawk’ wheat. Some isolates from Colorado, Kansas and Nebraska had low relative titre in wheat compared with the 06-123 isolate. This information is important in critical selection of TriMV isolates for use in greenhouse and field studies and in resistance screening protocols.