Helena Grotto - Academia.edu (original) (raw)

Papers by Helena Grotto

Research paper thumbnail of O hemograma: importância para a interpretação da biópsia

Revista Brasileira De Hematologia E Hemoterapia, 2009

Research paper thumbnail of Hereditary Hemoglobinopathies in a Population from so

Research paper thumbnail of Diagnóstico laboratorial da deficiência de ferro

Revista Brasileira De Hematologia E Hemoterapia, Jun 1, 2010

Research paper thumbnail of Laboratory diagnosis of iron deficiency anemia

Revista Brasileira De Hematologia E Hemoterapia, Jan 6, 2010

Research paper thumbnail of Reticulocyte evaluation in α+-thalassemia

American Journal of Hematology, 2005

Although it is almost certain that α+‐thalassemia protects against malaria, the mechanisms for th... more Although it is almost certain that α+‐thalassemia protects against malaria, the mechanisms for that are still unknown. It has been suggested that an increased number of young circulating red blood cells in α+‐thalassemic children, as a result of some degree of ineffective erythropoiesis, could be related to the high frequencies of the α+‐thalassemic allele in malaria endemic areas. Reticulocyte evaluation in this condition, however, has been poorly performed so far. Our objective was to determine the reticulocyte number and maturation degree, in addition to the soluble transferrin receptor and serum erythropoietin levels, in α+‐thalassemia heterozygotes, comparing them with normal α‐genotype controls. One hundred twenty‐one α+‐thalassemia carriers (−α3.7/αα) and 249 controls (αα/αα), all of them with normal serum ferritin levels, were subclassified according to age (1–5, 6–10, 11–15, 16–20, and over 20 years old). Reticulocyte analyzes were carried out by flow cytometry and sTfR and s‐Epo levels determined by immunonephelometry and chemiluminescence, respectively. The comparisons did not show any significant difference between thalassemics and controls regarding the reticulocyte parameters [percentages and absolute values, P = 0.2643 and 0.5421; high, medium, and low maturation degree, P = 0.2579, 0.2196, and 0.4192; RET maturity index (RMI), P = 0.2471, respectively], as well as the s‐Epo levels (P = 0.5711). The sTfR concentrations were higher in the thalassemic group (P = 0.0001), but statistical significance was due only to the 1–5 and over 20 subgroups (P = 0.0082 and 0.0436, respectively). The results found here are compatible with a compensated erythropoiesis and do not confirm the hypothesis mentioned above. Am. J. Hematol. 81:68–70, 2006. © 2005 Wiley‐Liss, Inc.

Research paper thumbnail of Metabolismo do ferro: uma revisão sobre os principais mecanismos envolvidos em sua homeostase

Revista Brasileira De Hematologia E Hemoterapia, Oct 1, 2008

Research paper thumbnail of Iron metabolism: an overview on the main mechanisms involved in its homeostasis

Revista Brasileira De Hematologia E Hemoterapia, Oct 1, 2008

Research paper thumbnail of Blood cell analysis: the importance for biopsy interpretation

Revista Brasileira De Hematologia E Hemoterapia, 2009

Research paper thumbnail of HbH Disease Associated with the (--<sup>MED</sup>) Deletion in a Brazilian Black Woman

Acta Haematologica, 1992

Clinical, laboratory features and restriction enzyme DNA analysis are reported for a black Brazil... more Clinical, laboratory features and restriction enzyme DNA analysis are reported for a black Brazilian woman with HbH disease. The patient presented a thalassemic blood picture with HbH and Hb Bart’s. Gene mapping demonstrated the compound heterozygous state for the --MED and -α3.7 deletions.Copyright © 1992 S. Karger AG, Basel

Research paper thumbnail of High levels of neopterin and interleukin-3 in sickle cell disease patients

Journal of Clinical Laboratory Analysis, 2006

Research paper thumbnail of Alterações hematologicas e diturbios do metabolismo do ferro em pacientes infectados pelo HIV

Research paper thumbnail of Platelet and reticulocyte new parameters: why and how to use them?

Revista Brasileira de Hematologia e Hemoterapia, 2016

Research paper thumbnail of O hemograma: importância para a interpretação da biópsia

Revista Brasileira de Hematologia e Hemoterapia, 2009

Research paper thumbnail of Metabolismo do ferro: uma revisão sobre os principais mecanismos envolvidos em sua homeostase

Revista Brasileira de Hematologia e Hemoterapia, 2008

Research paper thumbnail of Laboratory assessment of iron status and reticulocyte parameters in differential diagnosis of iron deficiency anemia and heterozygous beta-thalassemia

Jornal Brasileiro de Patologia e Medicina Laboratorial, 2002

Research paper thumbnail of Hematological and Biochemical Markers of Iron Status in a Male, Young, Physically Active Population

BioMed Research International, 2014

The aim of this study was to establish reference intervals (RIs) for the hemogram and iron status... more The aim of this study was to establish reference intervals (RIs) for the hemogram and iron status biomarkers in a physically active population. The study population included male volunteers (n=150) with an average age of 19 ± 1 years who had participated in a regular and controlled exercise program for four months. Blood samples were collected to determine hematological parameters using a Sysmex XE-5000 analyzer (Sysmex, Kobe, Japan). Iron, total iron-binding capacity (TIBC), transferrin saturation and ferritin, and high-sensitivity C-reactive protein (CRP) concentrations in serum samples were measured using commercial kits (Roche Diagnostics, GmbH, Mannheim, Germany) and a Roche/Hitachi 902 analyzer. The RIs were established using the RefVal program 4.1b. The leucocyte count, TIBC, and CRP and ferritin concentrations exhibited higher RIs compared with those in a nonphysically active population. Thirty volunteers (outliers) were removed from the reference population due to blood abn...

Research paper thumbnail of Clinical aspects and complete blood counts in children exposed to HIV-1: comparison between infected patients and seroreverters

Jornal de Pediatria, 2001

Research paper thumbnail of Aspectos clínicos e o hemograma em crianças expostas ao HIV-1: comparação entre pacientes infectados e soro-reversores

Jornal de Pediatria, 2001

Research paper thumbnail of Reticulocyte parameters in hemoglobinopathies and iron deficiency anemia

Revista Brasileira de Hematologia e Hemoterapia, 2003

Research paper thumbnail of Fisiologia e metabolismo do ferro

Revista Brasileira de Hematologia e Hemoterapia, 2010

Research paper thumbnail of O hemograma: importância para a interpretação da biópsia

Revista Brasileira De Hematologia E Hemoterapia, 2009

Research paper thumbnail of Hereditary Hemoglobinopathies in a Population from so

Research paper thumbnail of Diagnóstico laboratorial da deficiência de ferro

Revista Brasileira De Hematologia E Hemoterapia, Jun 1, 2010

Research paper thumbnail of Laboratory diagnosis of iron deficiency anemia

Revista Brasileira De Hematologia E Hemoterapia, Jan 6, 2010

Research paper thumbnail of Reticulocyte evaluation in α+-thalassemia

American Journal of Hematology, 2005

Although it is almost certain that α+‐thalassemia protects against malaria, the mechanisms for th... more Although it is almost certain that α+‐thalassemia protects against malaria, the mechanisms for that are still unknown. It has been suggested that an increased number of young circulating red blood cells in α+‐thalassemic children, as a result of some degree of ineffective erythropoiesis, could be related to the high frequencies of the α+‐thalassemic allele in malaria endemic areas. Reticulocyte evaluation in this condition, however, has been poorly performed so far. Our objective was to determine the reticulocyte number and maturation degree, in addition to the soluble transferrin receptor and serum erythropoietin levels, in α+‐thalassemia heterozygotes, comparing them with normal α‐genotype controls. One hundred twenty‐one α+‐thalassemia carriers (−α3.7/αα) and 249 controls (αα/αα), all of them with normal serum ferritin levels, were subclassified according to age (1–5, 6–10, 11–15, 16–20, and over 20 years old). Reticulocyte analyzes were carried out by flow cytometry and sTfR and s‐Epo levels determined by immunonephelometry and chemiluminescence, respectively. The comparisons did not show any significant difference between thalassemics and controls regarding the reticulocyte parameters [percentages and absolute values, P = 0.2643 and 0.5421; high, medium, and low maturation degree, P = 0.2579, 0.2196, and 0.4192; RET maturity index (RMI), P = 0.2471, respectively], as well as the s‐Epo levels (P = 0.5711). The sTfR concentrations were higher in the thalassemic group (P = 0.0001), but statistical significance was due only to the 1–5 and over 20 subgroups (P = 0.0082 and 0.0436, respectively). The results found here are compatible with a compensated erythropoiesis and do not confirm the hypothesis mentioned above. Am. J. Hematol. 81:68–70, 2006. © 2005 Wiley‐Liss, Inc.

Research paper thumbnail of Metabolismo do ferro: uma revisão sobre os principais mecanismos envolvidos em sua homeostase

Revista Brasileira De Hematologia E Hemoterapia, Oct 1, 2008

Research paper thumbnail of Iron metabolism: an overview on the main mechanisms involved in its homeostasis

Revista Brasileira De Hematologia E Hemoterapia, Oct 1, 2008

Research paper thumbnail of Blood cell analysis: the importance for biopsy interpretation

Revista Brasileira De Hematologia E Hemoterapia, 2009

Research paper thumbnail of HbH Disease Associated with the (--<sup>MED</sup>) Deletion in a Brazilian Black Woman

Acta Haematologica, 1992

Clinical, laboratory features and restriction enzyme DNA analysis are reported for a black Brazil... more Clinical, laboratory features and restriction enzyme DNA analysis are reported for a black Brazilian woman with HbH disease. The patient presented a thalassemic blood picture with HbH and Hb Bart’s. Gene mapping demonstrated the compound heterozygous state for the --MED and -α3.7 deletions.Copyright © 1992 S. Karger AG, Basel

Research paper thumbnail of High levels of neopterin and interleukin-3 in sickle cell disease patients

Journal of Clinical Laboratory Analysis, 2006

Research paper thumbnail of Alterações hematologicas e diturbios do metabolismo do ferro em pacientes infectados pelo HIV

Research paper thumbnail of Platelet and reticulocyte new parameters: why and how to use them?

Revista Brasileira de Hematologia e Hemoterapia, 2016

Research paper thumbnail of O hemograma: importância para a interpretação da biópsia

Revista Brasileira de Hematologia e Hemoterapia, 2009

Research paper thumbnail of Metabolismo do ferro: uma revisão sobre os principais mecanismos envolvidos em sua homeostase

Revista Brasileira de Hematologia e Hemoterapia, 2008

Research paper thumbnail of Laboratory assessment of iron status and reticulocyte parameters in differential diagnosis of iron deficiency anemia and heterozygous beta-thalassemia

Jornal Brasileiro de Patologia e Medicina Laboratorial, 2002

Research paper thumbnail of Hematological and Biochemical Markers of Iron Status in a Male, Young, Physically Active Population

BioMed Research International, 2014

The aim of this study was to establish reference intervals (RIs) for the hemogram and iron status... more The aim of this study was to establish reference intervals (RIs) for the hemogram and iron status biomarkers in a physically active population. The study population included male volunteers (n=150) with an average age of 19 ± 1 years who had participated in a regular and controlled exercise program for four months. Blood samples were collected to determine hematological parameters using a Sysmex XE-5000 analyzer (Sysmex, Kobe, Japan). Iron, total iron-binding capacity (TIBC), transferrin saturation and ferritin, and high-sensitivity C-reactive protein (CRP) concentrations in serum samples were measured using commercial kits (Roche Diagnostics, GmbH, Mannheim, Germany) and a Roche/Hitachi 902 analyzer. The RIs were established using the RefVal program 4.1b. The leucocyte count, TIBC, and CRP and ferritin concentrations exhibited higher RIs compared with those in a nonphysically active population. Thirty volunteers (outliers) were removed from the reference population due to blood abn...

Research paper thumbnail of Clinical aspects and complete blood counts in children exposed to HIV-1: comparison between infected patients and seroreverters

Jornal de Pediatria, 2001

Research paper thumbnail of Aspectos clínicos e o hemograma em crianças expostas ao HIV-1: comparação entre pacientes infectados e soro-reversores

Jornal de Pediatria, 2001

Research paper thumbnail of Reticulocyte parameters in hemoglobinopathies and iron deficiency anemia

Revista Brasileira de Hematologia e Hemoterapia, 2003

Research paper thumbnail of Fisiologia e metabolismo do ferro

Revista Brasileira de Hematologia e Hemoterapia, 2010