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Papers by Helene Golembiovska

The composition of volatile components in the spikes, leaves, stems and roots of Prunella vulgari... more The composition of volatile components in the spikes, leaves, stems and roots of Prunella vulgaris L. (Lamiaceae) grown in Ukraine was studied using gas chromatography-mass spectrometry. The main compound of volatile components was found to be squalene, myristic acid, spathulenol, viridiflorol, germacrone. Different anatomical parts also showed altered volatile profiles.

The polyphenolic composition of Prunella vulgaris L. from Ukraine was analyzed by HPLC with photo... more The polyphenolic composition of Prunella vulgaris L. from Ukraine was analyzed by HPLC with photodiodearray detection system. A total of 14 phenolic compounds (rosmarinic acid, chlorogenic acid, gallic acid, ellagic acid, ferulic acid, caffeic acid, rutin, quercetin, hyperoside, apigenin-7-glucoside, apigenin, luteolin, quercitrin, and isoquercitrin) were identified and quantified.

Ключові слова: мінеральні речовини, суховершки звичайні. Ключевые слова: минеральные вещества, че... more Ключові слова: мінеральні речовини, суховершки звичайні. Ключевые слова: минеральные вещества, черноголовка обыкновенная.

Common self-heal (Prunella vulgaris L.) is a pervasive weed with undervalued potentialof pharmaco... more Common self-heal (Prunella vulgaris L.) is a pervasive weed with undervalued potentialof pharmacological activity. Traditional medicine of China uses this herb as a hepatoprotective, anti-inflammatory and immunomodulatory agent. Sulfated polysaccharide previously isolated from the common self-heal herb, has shown antiviral properties (inhibition of viral replication of herpes 1st and 2nd types, HIV, etc.). [2]. However, despite the complicated flow chart of its receipt, the study of the structure and physical and chemical properties of the polysaccharide was carried out.

By the method of microscopy, thin-layer chromatography, UV/VIS spectrophotometry and HPLC the qua... more By the method of microscopy, thin-layer chromatography, UV/VIS spectrophotometry and HPLC the quality composition and quantitative maintenance of anthocyanins of above-ground and underground organs of common self-heal (Prunella vulgaris L.) were established. The highest amount of anthocyanins was found in prunella inflorescences (spikes) and the lowest one -in leaves. The histochemical studies of Prunella vulgaris L. fresh inflorescences cup and bracts transverse sections found that cups accumulate anthocyanins and permeated by vascular passages. The results of spectrophotometric pH differential assay showed the highest monomeric anthocyanin pigment concentration, as paeonidin-3-glucoside, color density and percent of polymeric color for selfheal inflorescences. Chromatographic analysis has shown a large difference between qualitative and quantitative composition in different parts of self-heal. Paeonidin and cyanidin glycosides were the major anthocyanin derivatives identified in spikes and stems of self-heal, especially the 3-O-glucosides and 3-O-rutinoside. The most representative anthocyanin in prunella roots were delphinidin-3-O-galactoside. This is the first study regarding anthocyanin composition in Prunella vulgaris L.

The composition of volatile components in the spikes, leaves, stems and roots of Prunella vulgari... more The composition of volatile components in the spikes, leaves, stems and roots of Prunella vulgaris L. (Lamiaceae) grown in Ukraine was studied using gas chromatography-mass spectrometry. The main compound of volatile components was found to be squalene, myristic acid, spathulenol, viridiflorol, germacrone. Different anatomical parts also showed altered volatile profiles.

The polyphenolic composition of Prunella vulgaris L. from Ukraine was analyzed by HPLC with photo... more The polyphenolic composition of Prunella vulgaris L. from Ukraine was analyzed by HPLC with photodiodearray detection system. A total of 14 phenolic compounds (rosmarinic acid, chlorogenic acid, gallic acid, ellagic acid, ferulic acid, caffeic acid, rutin, quercetin, hyperoside, apigenin-7-glucoside, apigenin, luteolin, quercitrin, and isoquercitrin) were identified and quantified.

Ключові слова: мінеральні речовини, суховершки звичайні. Ключевые слова: минеральные вещества, че... more Ключові слова: мінеральні речовини, суховершки звичайні. Ключевые слова: минеральные вещества, черноголовка обыкновенная.

Common self-heal (Prunella vulgaris L.) is a pervasive weed with undervalued potentialof pharmaco... more Common self-heal (Prunella vulgaris L.) is a pervasive weed with undervalued potentialof pharmacological activity. Traditional medicine of China uses this herb as a hepatoprotective, anti-inflammatory and immunomodulatory agent. Sulfated polysaccharide previously isolated from the common self-heal herb, has shown antiviral properties (inhibition of viral replication of herpes 1st and 2nd types, HIV, etc.). [2]. However, despite the complicated flow chart of its receipt, the study of the structure and physical and chemical properties of the polysaccharide was carried out.

By the method of microscopy, thin-layer chromatography, UV/VIS spectrophotometry and HPLC the qua... more By the method of microscopy, thin-layer chromatography, UV/VIS spectrophotometry and HPLC the quality composition and quantitative maintenance of anthocyanins of above-ground and underground organs of common self-heal (Prunella vulgaris L.) were established. The highest amount of anthocyanins was found in prunella inflorescences (spikes) and the lowest one -in leaves. The histochemical studies of Prunella vulgaris L. fresh inflorescences cup and bracts transverse sections found that cups accumulate anthocyanins and permeated by vascular passages. The results of spectrophotometric pH differential assay showed the highest monomeric anthocyanin pigment concentration, as paeonidin-3-glucoside, color density and percent of polymeric color for selfheal inflorescences. Chromatographic analysis has shown a large difference between qualitative and quantitative composition in different parts of self-heal. Paeonidin and cyanidin glycosides were the major anthocyanin derivatives identified in spikes and stems of self-heal, especially the 3-O-glucosides and 3-O-rutinoside. The most representative anthocyanin in prunella roots were delphinidin-3-O-galactoside. This is the first study regarding anthocyanin composition in Prunella vulgaris L.