Hema Chandran - Academia.edu (original) (raw)

Papers by Hema Chandran

Research paper thumbnail of Plant Growth-Promoting Rhizobacteria as a Green Alternative for Sustainable Agriculture

Sustainability, 2021

Environmental stress is a major challenge for sustainable food production as it reduces yield by ... more Environmental stress is a major challenge for sustainable food production as it reduces yield by generating reactive oxygen species (ROS) which pose a threat to cell organelles and biomolecules such as proteins, DNA, enzymes, and others, leading to apoptosis. Plant growth-promoting rhizobacteria (PGPR) offers an eco-friendly and green alternative to synthetic agrochemicals and conventional agricultural practices in accomplishing sustainable agriculture by boosting growth and stress tolerance in plants. PGPR inhabit the rhizosphere of soil and exhibit positive interaction with plant roots. These organisms render multifaceted benefits to plants by several mechanisms such as the release of phytohormones, nitrogen fixation, solubilization of mineral phosphates, siderophore production for iron sequestration, protection against various pathogens, and stress. PGPR has the potential to curb the adverse effects of various stresses such as salinity, drought, heavy metals, floods, and other st...

Research paper thumbnail of Microbial Biodiversity and Bioremediation Assessment Through Omics Approaches

Frontiers in Environmental Chemistry, 2020

Industrialization, population burst, and changing lifestyles have resulted in the genesis of non-... more Industrialization, population burst, and changing lifestyles have resulted in the genesis of non-degradable pollutants languishing the environment and human health. Biological approaches using microorganisms are gaining importance as an eco-friendly and cost-effective substitute to mitigate the pollution load. Microorganisms can survive in a divergent environment and produce metabolites that can degrade and transform pollutants making it possible to revive contaminated sites naturally. Modern omics technologies like metagenomics, transcriptomics, proteomics, etc. have been used nowadays design strategies to study ecology and diversity of microorganisms and their application in environmental monitoring and bioremediation. The present article will focus on the omics techniques reportedly used in environmental monitoring to tackle the pollution load.

Research paper thumbnail of Plant tissue culture as a perpetual source for production of industrially important bioactive compounds

Biotechnology Reports, 2020

Plants have been used throughout the world for its medicinal powers since ancient time. The pharm... more Plants have been used throughout the world for its medicinal powers since ancient time. The pharmacological properties of plants are based on their phytochemical components especially the secondary metabolites which are outstanding sources of value added bioactive compounds. Secondary metabolites have complex chemical composition and are produced in response to various forms of stress to perform different physiological tasks in plants. They are used in pharmaceutical industries, cosmetics, dietary supplements, fragrances, flavors, dyes, etc. Extended use of these metabolites in various industrial sectors has initiated a need to focus research on increasing the production by employing plant tissue culture (PTC) techniques and optimizing their large scale production using bioreactors. PTC techniques being independent of climatic and geographical conditions will provide an incessant, sustainable, economical and viable production of secondary metabolites. This review article intends to assess the advantages of using plant tissue culture, distribution of important secondary metabolites in plant families, strategies involved for optimal metabolite production and the industrial importance of selected secondary metabolites.

Research paper thumbnail of Exopolysaccharide (EPS)-producing bacteria: an ideal source of biopolymers

The handbook of microbial bioresources

Research paper thumbnail of Bioprospecting of Traditional Sweet Manufacturing Effluent for Exopolysachharide Producing Bacteria and Their Biotechnological Applications

International Journal of Applied Sciences and Biotechnology, 2015

This work was aimed to isolate, purify, characterize and to study the biological applications of ... more This work was aimed to isolate, purify, characterize and to study the biological applications of extracellular polysaccharide (EPS) produced by Agrobacterium fabrum strain C 58 isolated from effluent of a traditional sweet manufacturing unit. The isolated bacterium Agrobacterium fabrum strain C 58 was found to produce 16.21 g/L crude EPS in terms dry weight at 28 0 C when brown sugar was supplemented as the source of carbon. The polysachharide was further purified by anion exchange chromatography on a column of DEAE Cellulose-52 yielding one fraction which eluted at 0.2M NaCl. The monosachharide composition of EPS by TLC indicated it to be a hetero polysachharide composed of glucose, galactose, mannose and rhamnose. The FT-IR analysis proves the presence of biologically important functional groups and alpha glycosidic linkage between individual glycosyl residues. The biopolymer at a concentration of 1 % exhibited significant lipid emulsifying capacity against various vegetable oils. The effectiveness of polysaccharide in inhibiting free radicals evaluated by DPPH radical scavenging appeared to be significant. This is the first report about isolation of potent EPS producers from a traditional sweet manufacturing unit effluent which confirms that these samples can be used as a potential habitat for bioprospecting extracellular polymer producing bacteria. The diversity offered by microorganisms in these diverse habitats thus renders a hope for screening new habitats for isolating and developing new polysaccharides with properties superior to those of the existing polymers.

Research paper thumbnail of Plant Growth-Promoting Rhizobacteria as a Green Alternative for Sustainable Agriculture

Sustainability, 2021

Environmental stress is a major challenge for sustainable food production as it reduces yield by ... more Environmental stress is a major challenge for sustainable food production as it reduces yield by generating reactive oxygen species (ROS) which pose a threat to cell organelles and biomolecules such as proteins, DNA, enzymes, and others, leading to apoptosis. Plant growth-promoting rhizobacteria (PGPR) offers an eco-friendly and green alternative to synthetic agrochemicals and conventional agricultural practices in accomplishing sustainable agriculture by boosting growth and stress tolerance in plants. PGPR inhabit the rhizosphere of soil and exhibit positive interaction with plant roots. These organisms render multifaceted benefits to plants by several mechanisms such as the release of phytohormones, nitrogen fixation, solubilization of mineral phosphates, siderophore production for iron sequestration, protection against various pathogens, and stress. PGPR has the potential to curb the adverse effects of various stresses such as salinity, drought, heavy metals, floods, and other st...

Research paper thumbnail of Microbial Biodiversity and Bioremediation Assessment Through Omics Approaches

Frontiers in Environmental Chemistry, 2020

Industrialization, population burst, and changing lifestyles have resulted in the genesis of non-... more Industrialization, population burst, and changing lifestyles have resulted in the genesis of non-degradable pollutants languishing the environment and human health. Biological approaches using microorganisms are gaining importance as an eco-friendly and cost-effective substitute to mitigate the pollution load. Microorganisms can survive in a divergent environment and produce metabolites that can degrade and transform pollutants making it possible to revive contaminated sites naturally. Modern omics technologies like metagenomics, transcriptomics, proteomics, etc. have been used nowadays design strategies to study ecology and diversity of microorganisms and their application in environmental monitoring and bioremediation. The present article will focus on the omics techniques reportedly used in environmental monitoring to tackle the pollution load.

Research paper thumbnail of Plant tissue culture as a perpetual source for production of industrially important bioactive compounds

Biotechnology Reports, 2020

Plants have been used throughout the world for its medicinal powers since ancient time. The pharm... more Plants have been used throughout the world for its medicinal powers since ancient time. The pharmacological properties of plants are based on their phytochemical components especially the secondary metabolites which are outstanding sources of value added bioactive compounds. Secondary metabolites have complex chemical composition and are produced in response to various forms of stress to perform different physiological tasks in plants. They are used in pharmaceutical industries, cosmetics, dietary supplements, fragrances, flavors, dyes, etc. Extended use of these metabolites in various industrial sectors has initiated a need to focus research on increasing the production by employing plant tissue culture (PTC) techniques and optimizing their large scale production using bioreactors. PTC techniques being independent of climatic and geographical conditions will provide an incessant, sustainable, economical and viable production of secondary metabolites. This review article intends to assess the advantages of using plant tissue culture, distribution of important secondary metabolites in plant families, strategies involved for optimal metabolite production and the industrial importance of selected secondary metabolites.

Research paper thumbnail of Exopolysaccharide (EPS)-producing bacteria: an ideal source of biopolymers

The handbook of microbial bioresources

Research paper thumbnail of Bioprospecting of Traditional Sweet Manufacturing Effluent for Exopolysachharide Producing Bacteria and Their Biotechnological Applications

International Journal of Applied Sciences and Biotechnology, 2015

This work was aimed to isolate, purify, characterize and to study the biological applications of ... more This work was aimed to isolate, purify, characterize and to study the biological applications of extracellular polysaccharide (EPS) produced by Agrobacterium fabrum strain C 58 isolated from effluent of a traditional sweet manufacturing unit. The isolated bacterium Agrobacterium fabrum strain C 58 was found to produce 16.21 g/L crude EPS in terms dry weight at 28 0 C when brown sugar was supplemented as the source of carbon. The polysachharide was further purified by anion exchange chromatography on a column of DEAE Cellulose-52 yielding one fraction which eluted at 0.2M NaCl. The monosachharide composition of EPS by TLC indicated it to be a hetero polysachharide composed of glucose, galactose, mannose and rhamnose. The FT-IR analysis proves the presence of biologically important functional groups and alpha glycosidic linkage between individual glycosyl residues. The biopolymer at a concentration of 1 % exhibited significant lipid emulsifying capacity against various vegetable oils. The effectiveness of polysaccharide in inhibiting free radicals evaluated by DPPH radical scavenging appeared to be significant. This is the first report about isolation of potent EPS producers from a traditional sweet manufacturing unit effluent which confirms that these samples can be used as a potential habitat for bioprospecting extracellular polymer producing bacteria. The diversity offered by microorganisms in these diverse habitats thus renders a hope for screening new habitats for isolating and developing new polysaccharides with properties superior to those of the existing polymers.