Hemant Dhongade - Academia.edu (original) (raw)

Papers by Hemant Dhongade

Steroids, 2019

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

The medicinal values of plants lie in the bioactive phytochemical constituents that produce defin... more The medicinal values of plants lie in the bioactive phytochemical constituents that produce definite physiological effects on the human body. Though the traditional Indian system of medicine has a long history of use, they lack adequate scientific documentation, particularly in light of modern scientific knowledge1. These natural compounds formed the base of modern drugs as we are using today2,3,4. “Phyto ” is the Greek word for plant. There are many families of phytochemicals

antioxidant, wound healing, anti- inflammatory Correspondence to author:

Research Journal of Pharmacology and Pharmacodynamics, 2010

The present study deals with evaluation of antioxidant, wound healing and anti-inflammatory activ... more The present study deals with evaluation of antioxidant, wound healing and anti-inflammatory activity of ethanolic extract of Curcuma longa Linn rhizomes. The ethanolic extract prepared by maceration technique was subjected to screen for antioxidant activity using DPPH radical scavenging method and wound healing activity using incision, excision, histopathological and dead space wound model and the study was supported with evaluation of granuloma tissue to estimate hydroxyproline content and histopathological evaluation. The anti-inflammatory study was carried out by using carageenan induced rat paw odema method. The tested extract of different dilutions in range 200μg/ml to 1000 μg/ml shows activity in range of 9.34% to 18.55%. Significant increase in wound closure rate, skin breaking strength, granuloma breaking strength was observed. The hydroxyproline content was also increased with decrease in scar area. The initial healing action might be due to increased collagen deposition an...

The plant Achyranthes aspera is widely used in Ayurvedic system of medicine as Anti-diabetic, Ant... more The plant Achyranthes aspera is widely used in Ayurvedic system of medicine as Anti-diabetic, Anti-asthmatic, diuretic, Antiviral, Anti-carcinogenic, tooth brush, appetizer and cure gastric disorders. Roots are used in treatment of snakebites. Through literature survey it has been revealed that the paste of the roots of the plant is beneficial in treatment of ringworm infection. Thus, the study is carried out in order to determine the antifungal (ringworm) activity of the roots of the plant. The roots of the plant are subjected to extraction with water and ethanol. The antifungal activity was carried out using agar well diffusion method. All the plant extracts were compared with standard Clotrimazole (1%w/w) as positive control and distilled water, ethanol used as negative control. Accordingly ethanolic extract of the roots was found to more effective against Trichophyton rubrum as compared to aqueous extract.

Oriental Journal of Chemistry, 2016

Nardostachys jatamansi DC. is an endangered, primitive and therapeutic herbal agent belonging to ... more Nardostachys jatamansi DC. is an endangered, primitive and therapeutic herbal agent belonging to family Valerianaceae. The rhizomes of this hairy, perennial, dwarf and herbaceous plant are used for therapeutic effect in ayurvedic and unani system of medicine. Nardostachys jatamansi has been reported to have many therapeutic activities like antifungal, antimicrobial, antioxidant, hepatoprotective and cardio protective properties. It is used in the treatment of insomnia and CNS disorders. The vasodilator, bronchodilator, spasmolytic and platelet aggregation inhibition activities of the plant have also been reported. Jatamansone, nardostachone and actinidine are the major secondary metabolites present in the plant. This review article is summary of the potential benefits of this medicinal plant as reported in literature. The review also highlights the need for the use of this plant in Ayurvedic system of medicine and future prospects for further research.

International Journal of Biomedical and Advance Research, 2011

Objective: Hypolipidemic activity of ethanolic extract of Daucus carota seeds in Normal rats. Met... more Objective: Hypolipidemic activity of ethanolic extract of Daucus carota seeds in Normal rats. Method: Rats were divided in to 4 groups. First control group, treated with vehicle only for 7 days. Second group was standard, treated with lovastatin (7.2mg/kg) for 7 days. Third and fourth group were test, treated with Daucus carota seeds extract (200mg/kg and 400mg/kg) for 7 days. 8 th day blood sample was collected by retro orbital route and lipid profiles were estimated in serum. Result and Conclusions: Standard and test (400mg/kg DCSE) groups were showed significant (P <0.001) reduction in the total cholesterol, triglyceride HDL and VLDL as compared control group. Test group (200mg/kg) was showed significant (P <0.05) reduction as compared control group. The antioxidant potential of Daucus carota seeds has contributed to the reduction of oxidative stress and Lipid levels in experimental rats.

Int J Pharm Sci Res, 2010

... R, Latha PG, Arafat MM, Shyamal S, Shine VJ, Anuja GI, Suja SR and Rajasekharan S: Anti-infla... more ... R, Latha PG, Arafat MM, Shyamal S, Shine VJ, Anuja GI, Suja SR and Rajasekharan S: Anti-inflamatory, analgesic and anti-lipid peroxidation studies on stem bark of Ficus religiosa Linn. Natural Product Radiance 2007; 6(5), 377-81. 3. Warrier PK, Nambiar VPK, Ramankutty C ...

Journal of Pharmacopuncture

Moringa oleifera Lam. or munga is one of the most important plant widely cultivated in India. It ... more Moringa oleifera Lam. or munga is one of the most important plant widely cultivated in India. It belongs to family Moringaceae. This plant is widely used as nutritional herb and contains valuable pharmacological action like anti-asthmatic, anti-diabetic, hepatoprotective, anti-inflammatory, anti-fertility, anti-cancer, antimicrobial, anti-oxidant, cardiovascular, anti-ulcer, CNS activity, anti-allergic, wound healing, analgesic, and antipyretic activity, Moringa oleifera Lam. The plant is also known as Horseradish tree, Drumstick tree. Every part of this plant contains a valuable medicinal feature. It contain rich source of the vitamin A, vitamin C and milk protein. Different types of active phytoconstituents like alkaloids, protein, quinine, saponins, flavonoids, tannin, steroids, glycosides, fixed oil and fats are present. This plant is also found in the tropical regions. Some other constituents are niazinin A, niazinin B and niazimicin A, niaziminin B. The present review discusses the phytochemical composition, medicinal uses & pharmacological activity of this plant.

Indian Journal of Pharmaceutical Education and Research

Objective: Evaluation of the cytotoxic effect of different extracts of Orthosiphon pallidus Royle... more Objective: Evaluation of the cytotoxic effect of different extracts of Orthosiphon pallidus Royle on MCF-7 cell lines, comparatively. Materials and Methods: The breast cell line MCF-7 was cultured in DMEM medium containing fetal bovine serum & antibiotics. The cells were exposed to different doses of all the ethanolic, aqueous and hydroalcoholic extracts and incubated for 24, 48 and 72 hrs respectively and further studied for MTT colorimetric test, SRB test, glutathione assay and cellular uptake assay. Results: The results showed hydroalcoholic extract had a dose and time-dependent anticancer effect on MCF-7 cancer cells after 72 hours (P<0.05). The study revealed that Orthosiphon pallidus Royle could inhibit the growth of cancerous cells. Conclusion: The ethanolic, aqueous and hydroalcoholic extracts of Orthosiphon pallidus Royle exhibited anticancer activity in a wide range in dose-dependent manner. The study concluded that Orthosiphon pallidus Royle shows cellular uptake and anti-proliferative activities.

Journal of pharmacopuncture, 2017

The current investigation was carried out to determine the cytotoxic and the antimicrobial activi... more The current investigation was carried out to determine the cytotoxic and the antimicrobial activities of methanolic extracts of Plumbago zeylanica. The stems, leaves, and whole plants were air dried and extracted with methanol by using a Soxhlet extractor for 72 hours at 55 - 60°C. The antimicrobial activities were determined from the zones of inhibition, which were measured by using the agar well diffusion method, and the cytotoxicity assays were performed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay method. The methanolic extracts of the stem and the leaves of Plumbago zeylanica were tested against six bacterial species and nine fungal species, and both extracts showed antimicrobial activity in a dose-dependent manner. The leaf extract of Plumbago zeylanica showed maximum antimicrobial activity against both Staphylococcus aureus sub sp aureus and Fusarium oxysporum. The stem extract was found to be more antimicrobial against the Pseudomonas ae...

International journal of biological macromolecules, 2017

The effect of Ca(2+) ion cross-linker on acryalamide grafted carboxymethyl xanthan gum (CMXG-g-PA... more The effect of Ca(2+) ion cross-linker on acryalamide grafted carboxymethyl xanthan gum (CMXG-g-PAAm) on the drug release was investigated. Previously, CMXG was synthesized from XG and further grafted to CMXG-g-PAAm to retard the drug release. Once the CaCl2 solution is added to CMXG-g-PAAm, Ca(2+) considerably affected the drug release mechanism mainly by diffusion and erosion. In order to validate the grafted polymer, tablets were prepared using wet granulation and dry granulation methods It has been noticed that the tablets prepared by wet granulation successfully controls the release of the drug over an extended period of time. Moreover, the release profile was aligned to Korsmeyer-Peppas equation and exhibited the drug transport mechanism via diffusion and erosion.

Indian Journal of Pharmaceutical Education and Research, 2016

Background: Leaf juice as well as leaves of Barleria prionitis Linn and Barleria grandiflora Dalz... more Background: Leaf juice as well as leaves of Barleria prionitis Linn and Barleria grandiflora Dalz (Acanthaceae) is used by rural people across various regions of India in treatment of oral ailments such as dental troubles, gum ailments, pyorrhoea, dental carries and mouth ulcers. Methods: An antimicrobial activity was carried on aqueous and ethanolic extracts of both herbs and compared with standard chlorhexidine. Cell line study was carried using Human Gingival Fibroblast on ethanolic extracts of leaves of both the herbs and compared with standard marketed preparation Zyrtee. Cytotoxicity and anti gingivitis activity were evaluated using MTT assay. Results of cytokine secretion assay and cytokine measurement assay were analyzed using ANOVA followed by Dunnett multiple comparison test. P values less than 0.05 were considered as significant. Results: Results of antimicrobial activity suggest significant potency for ethanolic extracts of both the herbs. CTC50 values for ethanolic extracts was found to be 848 µg/ml and more than 1000 µg/ml respectively for Barleria prionitis and Barleria grandiflora. The cytokine secretion significantly increased by S. mutans control, whereas the cytokine level was reduced in a dose-dependent manner by ethanolic extracts in Streotococcus mutans stimulated HGF cells. The ethanolic extracts EBG and EBP showed 65.31383 ± 1.087%, 75.0425 ± 1.268% inhibition of TNF-α at tested concentration respectively. Conclusion: Thus based on findings of this study, we conclude the usefulness of Barleria prionitis and Barleria grandiflora in treatment of oral ailments such as gingivitis.

Medicinal Chemistry Research, 2016

International Journal of Biomedical and Advance Research, Jul 1, 2013

The aim of present work was to investigate the pharmacognostical and phytochemical properties of ... more The aim of present work was to investigate the pharmacognostical and phytochemical properties of Phyllanthus amarus leaves. The Ethno medicine involves the use of different plant extracts or the bioactive constituents of vital importance in the health application at an affordable cost and study of such ethanomedicine keenly represents one of the best avenues in searching new economic plants for medicine. Keeping this view in mind the present investigation was carried in Phyllanthus amarus leaves. Morphological study and pharmacognostical properties like transverse section, palisade ratio, stomatal number, stomatal index, vein islet number, and vein termination number were studied. Physical constants like ash value, extractive value, moisture content, swelling index was also studied. Qualitative phytochemical analysis of the plants confirms the presence of various phytochemicals like carbohydrates, alkaloids, glycosides, steroids, saponins, tannins, phenols and flavanoids.

International Journal of Biological Macromolecules, 2016

In the present work, an unreported graft copolymer of carboxymethyl xanthan gum and acrylamide ha... more In the present work, an unreported graft copolymer of carboxymethyl xanthan gum and acrylamide has been synthesised by free radical polymerisation in a nitrogen atmosphere using ammonium persulphate as an initiator. The optimum reaction conditions adopted for affording maximum percentage of grafting including its grafting efficiency were obtained by varying the concentration of carboxymethyl xanthan gum from 4 to 24gdm(-3); ammonium persulphate from 5×10(-4) to 30×10(-4)moldm(-3); acrylamide from 0.4 to 1.2moldm(-3); reaction temperature from 55 to 75°C and reaction time from 30 to 90min. The synthesised graft copolymer has been characterised by (1)H NMR, FTIR spectroscopy, X-ray diffraction measurement, thermal analysis, viscosity measurement and scanning electron microscopy. However, grafting of acrylamide onto carboxymethyl xanthan gum backbone enhanced its thermal stability. This graft copolymer might be well exploited globally as a potential carrier for drug delivery system.

... With the limitations of oral drug delivery and the pain and needle phobias associated ... PE ... more ... With the limitations of oral drug delivery and the pain and needle phobias associated ... PE Daddona, Macroflux microprojection array patch technology: a new and efficient approach for intracutaneous ... He further focuses his research activity in the other novel drug deliver system. ...